Rh5DG518700

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003566
Physical Location & Seq
Forward (+)
80580680 .. 80590086
9407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG518700.1

Sequence Viewer

Length: 1938 bp
ATGGTTCCTCACTATGACCCTGAAATGCGTGAAGTGAAGCGCTTTGCTGATCTCACTGTTGATGAGACCAGTGATCTTTGGATCACAGCACAAAAGGTTGGCAGTCAGCTTGAGAGCTACCACAAAGCAACATCTCTAACATTTGGGATTCAAGATGGTCCCCAAGCAGGGCAGACTGTACCCCATGTTCATATCCATATCCTTCCGCGCAAAGATGATGCACTTGATGAAAAGGAGAAGAAGAAGAAGCTTGACTTGGAAAAGGAGAGGAAGGACAGAACCCTAGAGGAAATGACACAAGAAGCAGAGGAATACAGAAAGCTTTTCTTTGTGCATGGATGGTTAACTATCATTCGTTTAGTTCCCCTACCAATAAAAACACTTCAGTCTAAAAATGGCGGGAGAAGTTTCCCTTTCACAGGTAATGTCTTGATCAAAAAAGACTCGAACCGGTTCTTCTCCAAAGTCCCAAACTCTACAGACCGTCTCAACAGCAGAGCAACCAGAAACGTCAAAATGTCGTCTATGCAGGCCATTCTCAAGGTAATGGCTTTGAAACAAAGAGGGATCAGAGCTGAGTATATGGGTAGTAGTCAAACTGATAGCACTGTCCAGAGCCGAGCCGAGAGTGGTCAATTTGATATCTTGTACATGACCCCTGAAAAGGCATGTAAGATTCCTGACAGCTTCTGGTCAAAATTACTAAGGGCTGGAATCTGCCTGTTTGCTGTTGATGAAGCACACTGCATATCGGAGTGGGGCCATGATTTCAGGATGGACTACAAGCGATTGGACAAGTTACGTGGCATTCTCGTTGGTGTCCCATTTATTGGCTTAACTGCAACTGCTACTAAAAAGGTTCAAATGGACATTGTTAATTCCTTGAAGATGAAAAACCCATATTACAGAATAGGCTCCTTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTCATGAACTAGCTCAAGAAGTTTCCAAATTTGTATGCACAGATGGTTCAACTATAATTTACTGCACGACAATTAAAGACGTTTATCAGGTATTCATTTCACTGAAGGAGGTGGCCATTAAGGCTGGAATCTACCATAGTGAGATGGATAATAAAGCTCGTGCCGAATCCCATAGATTATTTATAAGAGATGAAGTAGATGTCATGGTTGCTACTATTGCTTTCGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCATTATGGCTGCCCAAAGAGTTTGGAGTCTTATTACCAGGAAAGTGGACGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACCTCACAAAAGCTGAATTTTATGCTGGAGAGTGTCAAACAGAAAGTCGACGAAGAGCTGTTATAAAGTCGTTGAGGGCAGTACAACAATATTGCGTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTTACTTTGGGGAAATCTTTCCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCCCCAAAGAAGGAGATGGACATGTCTAGAGAAGCATATCTTCTAATGGCTTGCATTCAATCATGTCGGGGTACATGCGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCTCGGGCCAAAAAGATTCTTGCTGCCGAATATGACAAGCTTCCACTGCATGGACTTGGGAAAGACTATTCTGCAAATTGGTGGAAAACACTTGCTTACCAATTAATCTCTTATGGTTATTTGATGGAGATATACAGAAATGTAAGTGTCAGTCGGAAAGGGGAGCAATTTCTAAGTTCTGCTGGACCTGATCATCAACCGCCTCTAGTGTTGCCGGTGACCAGTGAAATGGTAGATGATGAGGACAATAAAAGTACATCAGGAGAGAAAGAGATGAAAATGTATGGAGTAGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

645

Amino Acids

73.5

Weight (kDa)

8.88

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HIT PF01230 10 - 72 4.4e-14 HIT domain
DEAD PF00270 188 - 288 1e-07 DEAD/DEAH box helicase
Helicase_C PF00271 339 - 435 2.6e-19 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 447 - 508 3.6e-16 RecQ zinc-binding
RQC PF09382 511 - 605 1.5e-20 RQC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1160, 1406
AccB7I CCANNNNNTGG 2 cut(s) 830, 1688
AccI GTMKAC 1 cut(s) 1390
AccII CGCG 1 cut(s) 208
AciI CCGC 4 cut(s) 206, 399, 1605, 1838
AclWI GGATC 2 cut(s) 89, 575
AcoI YGGCCR 1 cut(s) 1089
AcsI RAATTY 2 cut(s) 1004, 1358
AcuI CTGAAG 2 cut(s) 368, 1100
AfaI GTAC 5 cut(s) 180, 650, 1425, 1600, 1894
AfeI AGCGCT 1 cut(s) 41
AfiI CCNNNNNNNGG 7 cut(s) 167, 168, 419, 664, 830, 1537, 1688
AflIII ACRYGT 1 cut(s) 1548
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 7 cut(s) 152, 556, 863, 886, 958, 1026, 1586
AjnI CCWGG 1 cut(s) 1275
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
Alw26I GTCTC 2 cut(s) 59, 491
AlwI GGATC 2 cut(s) 89, 575
AlwNI CAGNNNCTG 1 cut(s) 690
Ama87I CYCGRG 1 cut(s) 1641
Aor51HI AGCGCT 1 cut(s) 41
AoxI GGCC 4 cut(s) 531, 760, 1089, 1644
ApeKI GCWGC 2 cut(s) 1248, 1661
ApoI RAATTY 2 cut(s) 1004, 1358
ArsI GACNNNNNNTTYG 2 cut(s) 1372, 1404
AseI ATTAAT 1 cut(s) 1742
AsiGI ACCGGT 1 cut(s) 450
Asp700I GAANNNNTTC 2 cut(s) 452, 1488
AspLEI GCGC 2 cut(s) 42, 210
AspS9I GGNCC 4 cut(s) 158, 760, 1644, 1823
AsuHPI GGTGA 1 cut(s) 1867
AvaI CYCGRG 1 cut(s) 1641
AvaII GGWCC 2 cut(s) 158, 1823
BalI TGGCCA 1 cut(s) 1091
BarI GAAGNNNNNNTAC 2 cut(s) 1614, 1646
BauI CACGAG 1 cut(s) 1134
BbvI GCAGC 2 cut(s) 1235, 1648
BccI CCATC 9 cut(s) 149, 333, 769, 1013, 1114, 1295, 1501, 1537, 1756
BciT130I CCWGG 1 cut(s) 1277
BclI TGATCA 2 cut(s) 432, 1828
BcoDI GTCTC 2 cut(s) 59, 491
BfaI CTAG 4 cut(s) 284, 986, 1554, 1844
BfmI CTRYAG 2 cut(s) 477, 1620
BfoI RGCGCY 1 cut(s) 43
BglI GCCNNNNNGGC 1 cut(s) 1097
BisI GCNGC 2 cut(s) 1249, 1662
BlsI GCNGC 2 cut(s) 1250, 1663
Bme1390I CCNGG 1 cut(s) 1277
Bme18I GGWCC 2 cut(s) 158, 1823
BmeT110I CYCGRG 1 cut(s) 1641
BmgT120I GGNCC 4 cut(s) 158, 760, 1644, 1823
BmiI GGNNCC 4 cut(s) 6, 160, 761, 916
BmrFI CCNGG 1 cut(s) 1277
BmsI GCATC 1 cut(s) 208
BpmI CTGGAG 1 cut(s) 1389
BpuEI CTTGAG 3 cut(s) 131, 524, 975
BsaAI YACGTR 1 cut(s) 803
BsaI GGTCTC 1 cut(s) 59
BsaWI WCCGGW 1 cut(s) 450
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 168, 419, 664, 830, 1537, 1688
Bse118I RCCGGY 2 cut(s) 450, 1852
Bse1I ACTGG 2 cut(s) 69, 1860
BseBI CCWGG 1 cut(s) 1277
BseGI GGATG 2 cut(s) 344, 780
BseLI CCNNNNNNNGG 7 cut(s) 167, 168, 419, 664, 830, 1537, 1688
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 2 cut(s) 69, 1860
BseXI GCAGC 2 cut(s) 1235, 1648
BsgI GTGCAG 1 cut(s) 1024
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 4 cut(s) 533, 762, 1091, 1646
BshTI ACCGGT 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 1641
BsiSI CCGG 2 cut(s) 451, 1853
BslFI GGGAC 3 cut(s) 144, 452, 806
BslI CCNNNNNNNGG 7 cut(s) 167, 168, 419, 664, 830, 1537, 1688
BsmAI GTCTC 2 cut(s) 59, 491
BsmBI CGTCTC 1 cut(s) 491
BsmFI GGGAC 3 cut(s) 144, 452, 806
BsmI GAATGC 2 cut(s) 807, 1581
BsnI GGCC 4 cut(s) 533, 762, 1091, 1646
Bso31I GGTCTC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 1641
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
BspACI CCGC 4 cut(s) 206, 399, 1605, 1838
BspANI GGCC 4 cut(s) 533, 762, 1091, 1646
BspCNI CTCAG 1 cut(s) 568
BspFNI CGCG 1 cut(s) 208
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 4 cut(s) 6, 160, 761, 916
BspPI GGATC 2 cut(s) 89, 575
BspQI GCTCTTC 1 cut(s) 1390
BspTNI GGTCTC 1 cut(s) 59
BsrFI RCCGGY 2 cut(s) 450, 1852
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 2 cut(s) 69, 1860
BssAI RCCGGY 2 cut(s) 450, 1852
BssMI GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
BssSI CACGAG 1 cut(s) 1134
Bst2BI CACGAG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 1277
Bst4CI ACNGT 4 cut(s) 58, 178, 485, 610
Bst6I CTCTTC 1 cut(s) 1390
BstAUI TGTACA 1 cut(s) 648
BstBAI YACGTR 1 cut(s) 803
BstC8I GCNNGC 3 cut(s) 531, 1322, 1579
BstDEI CTNAG 3 cut(s) 576, 704, 1811
BstEII GGTNACC 1 cut(s) 1855
BstENI CCTNNNNNAGG 1 cut(s) 417
BstF5I GGATG 2 cut(s) 344, 780
BstFNI CGCG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 2 cut(s) 42, 210
BstKTI GATC 7 cut(s) 52, 76, 84, 435, 570, 925, 1831
BstMAI GTCTC 2 cut(s) 59, 491
BstMBI GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
BstMWI GCNNNNNNNGC 3 cut(s) 1097, 1193, 1684
BstNI CCWGG 1 cut(s) 1277
BstNSI RCATGY 3 cut(s) 672, 1552, 1605
BstPI GGTNACC 1 cut(s) 1855
BstSCI CCNGG 1 cut(s) 1275
BstSFI CTRYAG 2 cut(s) 477, 1620
BstUI CGCG 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 1235, 1648
BstXI CCANNNNNNTGG 2 cut(s) 1283, 1867
BsuRI GGCC 4 cut(s) 533, 762, 1091, 1646
BtsCI GGATG 2 cut(s) 344, 780
BtsI GCAGTG 2 cut(s) 742, 1682
BtsIMutI CAGTG 7 cut(s) 54, 76, 606, 742, 1076, 1682, 1867
Cac8I GCNNGC 3 cut(s) 531, 1322, 1579
CaiI CAGNNNCTG 1 cut(s) 690
CciI TCATGA 1 cut(s) 979
CfoI GCGC 2 cut(s) 42, 210
Cfr10I RCCGGY 2 cut(s) 450, 1852
Cfr13I GGNCC 4 cut(s) 158, 760, 1644, 1823
Csp6I GTAC 5 cut(s) 179, 649, 1424, 1599, 1893
CspAI ACCGGT 1 cut(s) 450
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviQI GTAC 5 cut(s) 179, 649, 1424, 1599, 1893
DdeI CTNAG 3 cut(s) 576, 704, 1811
DpnI GATC 7 cut(s) 51, 75, 83, 434, 569, 924, 1830
DpnII GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
EaeI YGGCCR 1 cut(s) 1089
Eam1104I CTCTTC 1 cut(s) 1390
EarI CTCTTC 1 cut(s) 1390
Eco31I GGTCTC 1 cut(s) 59
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 2 cut(s) 158, 1823
Eco47III AGCGCT 1 cut(s) 41
Eco57I CTGAAG 2 cut(s) 368, 1100
Eco88I CYCGRG 1 cut(s) 1641
Eco91I GGTNACC 1 cut(s) 1855
EcoNI CCTNNNNNAGG 1 cut(s) 417
EcoO65I GGTNACC 1 cut(s) 1855
EcoRII CCWGG 1 cut(s) 1275
EcoRV GATATC 1 cut(s) 643
Esp3I CGTCTC 1 cut(s) 491
FalI AAGNNNNNCTT 8 cut(s) 239, 271, 311, 343, 397, 429, 1551, 1583
FaqI GGGAC 3 cut(s) 144, 452, 806
FauI CCCGC 1 cut(s) 392
FbaI TGATCA 2 cut(s) 432, 1828
FblI GTMKAC 1 cut(s) 1390
Fnu4HI GCNGC 2 cut(s) 1249, 1662
FokI GGATG 2 cut(s) 351, 787
Fsp4HI GCNGC 2 cut(s) 1249, 1662
FspBI CTAG 4 cut(s) 284, 986, 1554, 1844
GlaI GCGC 2 cut(s) 41, 209
GluI GCNGC 2 cut(s) 1249, 1662
GsuI CTGGAG 1 cut(s) 1389
HaeII RGCGCY 1 cut(s) 43
HaeIII GGCC 4 cut(s) 533, 762, 1091, 1646
HapII CCGG 2 cut(s) 451, 1853
HhaI GCGC 2 cut(s) 42, 210
Hin6I GCGC 2 cut(s) 40, 208
HinP1I GCGC 2 cut(s) 40, 208
HincII GTYRAC 2 cut(s) 345, 1391
HindII GTYRAC 2 cut(s) 345, 1391
HindIII AAGCTT 3 cut(s) 248, 320, 1676
HinfI GANTC 9 cut(s) 148, 443, 676, 714, 964, 1104, 1142, 1265, 1654
HpaI GTTAAC 1 cut(s) 345
HpaII CCGG 2 cut(s) 451, 1853
HphI GGTGA 1 cut(s) 1867
Hpy166II GTNNAC 3 cut(s) 345, 1286, 1391
Hpy188I TCNGA 6 cut(s) 572, 754, 963, 1498, 1611, 1794
Hpy188III TCNNGA 9 cut(s) 152, 430, 613, 680, 772, 980, 992, 1554, 1899
Hpy8I GTNNAC 3 cut(s) 345, 1286, 1391
Hpy99I CGWCG 1 cut(s) 1395
HpyAV CCTTC 5 cut(s) 212, 265, 1075, 1449, 1531
HpyCH4III ACNGT 4 cut(s) 58, 178, 485, 610
HpyCH4IV ACGT 3 cut(s) 510, 802, 1056
HpyF10VI GCNNNNNNNGC 3 cut(s) 1097, 1193, 1684
HpyF3I CTNAG 3 cut(s) 576, 704, 1811
HpySE526I ACGT 3 cut(s) 510, 802, 1056
HspAI GCGC 2 cut(s) 40, 208
Ksp22I TGATCA 2 cut(s) 432, 1828
KspAI GTTAAC 1 cut(s) 345
Kzo9I GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
LguI GCTCTTC 1 cut(s) 1390
LmnI GCTCC 2 cut(s) 920, 1801
Lsp1109I GCAGC 2 cut(s) 1235, 1648
LweI GCATC 1 cut(s) 208
MaeI CTAG 4 cut(s) 284, 986, 1554, 1844
MaeII ACGT 3 cut(s) 510, 802, 1056
MaeIII GTNAC 3 cut(s) 798, 1472, 1855
MalI GATC 7 cut(s) 51, 75, 83, 434, 569, 924, 1830
MboI GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
MboII GAAGA 8 cut(s) 250, 253, 256, 448, 898, 1407, 1559, 1622
MlsI TGGCCA 1 cut(s) 1091
MluNI TGGCCA 1 cut(s) 1091
MlyI GAGTC 3 cut(s) 437, 958, 1274
MmeI TCCRAC 1 cut(s) 1772
Mox20I TGGCCA 1 cut(s) 1091
MroXI GAANNNNTTC 2 cut(s) 452, 1488
MscI TGGCCA 1 cut(s) 1091
MseI TTAA 6 cut(s) 344, 836, 876, 1050, 1095, 1742
Msp20I TGGCCA 1 cut(s) 1091
MspI CCGG 2 cut(s) 451, 1853
MspR9I CCNGG 1 cut(s) 1277
Mva1269I GAATGC 2 cut(s) 807, 1581
MvaI CCWGG 1 cut(s) 1277
MvnI CGCG 1 cut(s) 208
MwoI GCNNNNNNNGC 3 cut(s) 1097, 1193, 1684
NdeII GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
NlaIV GGNNCC 4 cut(s) 6, 160, 761, 916
NmeAIII GCCGAG 2 cut(s) 644, 649
NmuCI GTSAC 1 cut(s) 1855
NspI RCATGY 3 cut(s) 672, 1552, 1605
PagI TCATGA 1 cut(s) 979
PciI ACATGT 1 cut(s) 1548
PciSI GCTCTTC 1 cut(s) 1390
PctI GAATGC 2 cut(s) 807, 1581
PdmI GAANNNNTTC 2 cut(s) 452, 1488
PfeI GAWTC 6 cut(s) 148, 676, 714, 1104, 1142, 1654
PflMI CCANNNNNTGG 2 cut(s) 830, 1688
PinAI ACCGGT 1 cut(s) 450
PkrI GCNGC 2 cut(s) 1250, 1663
PleI GAGTC 3 cut(s) 437, 958, 1273
PpsI GAGTC 3 cut(s) 437, 958, 1273
Ppu21I YACGTR 1 cut(s) 803
PscI ACATGT 1 cut(s) 1548
PshBI ATTAAT 1 cut(s) 1742
PsiI TTATAA 2 cut(s) 1160, 1406
Psp6I CCWGG 1 cut(s) 1275
PspEI GGTNACC 1 cut(s) 1855
PspGI CCWGG 1 cut(s) 1275
PspN4I GGNNCC 4 cut(s) 6, 160, 761, 916
PspPI GGNCC 4 cut(s) 158, 760, 1644, 1823
PsrI GAACNNNNNNTAC 2 cut(s) 171, 203
PstNI CAGNNNCTG 1 cut(s) 690
RsaI GTAC 5 cut(s) 180, 650, 1425, 1600, 1894
RsaNI GTAC 5 cut(s) 179, 649, 1424, 1599, 1893
SalI GTCGAC 1 cut(s) 1389
SapI GCTCTTC 1 cut(s) 1390
SaqAI TTAA 6 cut(s) 344, 836, 876, 1050, 1095, 1742
SatI GCNGC 2 cut(s) 1249, 1662
Sau3AI GATC 7 cut(s) 49, 73, 81, 432, 567, 922, 1828
Sau96I GGNCC 4 cut(s) 158, 760, 1644, 1823
SchI GAGTC 3 cut(s) 437, 958, 1274
ScrFI CCNGG 1 cut(s) 1277
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 2 cut(s) 477, 1620
SinI GGWCC 2 cut(s) 158, 1823
SmlI CTYRAG 3 cut(s) 110, 539, 990
SmoI CTYRAG 3 cut(s) 110, 539, 990
SsiI CCGC 4 cut(s) 206, 399, 1605, 1838
SspI AATATT 1 cut(s) 1433
SspMI CTAG 4 cut(s) 284, 986, 1554, 1844
StyD4I CCNGG 1 cut(s) 1275
TaaI ACNGT 4 cut(s) 58, 178, 485, 610
TaiI ACGT 3 cut(s) 513, 805, 1059
TaqI TCGA 2 cut(s) 446, 1390
TatI WGTACW 3 cut(s) 648, 1423, 1892
TfiI GAWTC 6 cut(s) 148, 676, 714, 1104, 1142, 1654
Tru1I TTAA 6 cut(s) 344, 836, 876, 1050, 1095, 1742
Tru9I TTAA 6 cut(s) 344, 836, 876, 1050, 1095, 1742
TscAI CASTG 7 cut(s) 61, 76, 613, 749, 1083, 1689, 1867
TseFI GTSAC 1 cut(s) 1855
TseI GCWGC 2 cut(s) 1248, 1661
Tsp45I GTSAC 1 cut(s) 1855
TspDTI ATGAA 9 cut(s) 179, 243, 750, 905, 968, 996, 1060, 1182, 1928
TspRI CASTG 7 cut(s) 61, 76, 613, 749, 1083, 1689, 1867
Van91I CCANNNNNTGG 2 cut(s) 830, 1688
VpaK11BI GGWCC 2 cut(s) 158, 1823
VspI ATTAAT 1 cut(s) 1742
XagI CCTNNNNNAGG 1 cut(s) 417
XapI RAATTY 2 cut(s) 1004, 1358
XbaI TCTAGA 1 cut(s) 1553
XceI RCATGY 3 cut(s) 672, 1552, 1605
XmiI GTMKAC 1 cut(s) 1390
XmnI GAANNNNTTC 2 cut(s) 452, 1488
XspI CTAG 4 cut(s) 284, 986, 1554, 1844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.