AT5G58240

HIT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003566
Physical Location & Seq
Reverse (-)
23558114 .. 23559353
1240 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58240.1

Sequence Viewer

Length: 543 bp
ATGCTGAACCTGCAAGTAACCGGGAAGACGATCTTATCTAGTATTCGTTGTCAGAGAAAGATGTCGTCTACTTGTTCTTCGTACGCATTTGGGCCGTACAAAATCGATCCCCGTGAAGTGTTCTACGCAACGCCTCTCTCTTATGCCATGGTTAATCTCCGTCCGCTTCTTCCTGCACATGTTCTTGTCTGCCCGAGACGCCTTGTGCCACGATTTACTGATCTTACAGCTGATGAGACCAGTGACCTGTGGCTTACTGCTCAGAAGGTTGGCTCTAAGCTTGAGACCTTCCATAATGCGTCTTCTCTCACATTAGCCATCCAAGATGGCCCTCAAGCGGGACAGACTGTTCCCCATGTACACATTCATATCTTGCCTCGTAAAGGTGGTGACTTTGAGAAGAATGATGAAATCTATGATGCTCTTGATGAGAAGGAGAAGGAACTGAAGCAGAAGCTTGATCTAGATAAAGACCGGGTTGATAGGAGCATTCAGGAGATGGCTGACGAGGCTTCCCAATACAGATCTCTTTTCGATTGCTAG

Protein Analysis

180

Amino Acids

20.4

Weight (kDa)

6.2

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HIT PF01230 37 - 128 9.6e-27 HIT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 68
AciI CCGC 2 cut(s) 164, 338
AclWI GGATC 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 199
AfaI GTAC 3 cut(s) 83, 98, 360
AfiI CCNNNNNNNGG 3 cut(s) 337, 338, 383
AflIII ACRYGT 1 cut(s) 178
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 3 cut(s) 230, 280, 457
AluI AGCT 3 cut(s) 230, 280, 457
Alw26I GTCTC 3 cut(s) 190, 230, 278
AlwI GGATC 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 193
AoxI GGCC 2 cut(s) 92, 328
AspS9I GGNCC 2 cut(s) 92, 329
AsuC2I CCSGG 2 cut(s) 22, 476
AsuHPI GGTGA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 193
BbsI GAAGAC 2 cut(s) 32, 294
BccI CCATC 3 cut(s) 320, 326, 493
BceAI ACGGC 1 cut(s) 79
BcnI CCSGG 2 cut(s) 22, 476
BcoDI GTCTC 3 cut(s) 190, 230, 278
BfaI CTAG 3 cut(s) 39, 464, 541
BfuAI ACCTGC 1 cut(s) 18
BglII AGATCT 1 cut(s) 524
Bme1390I CCNGG 2 cut(s) 22, 476
BmeT110I CYCGRG 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 92, 329
BmrFI CCNGG 2 cut(s) 22, 476
BmsI GCATC 1 cut(s) 409
BpiI GAAGAC 2 cut(s) 32, 294
BpuEI CTTGAG 2 cut(s) 302, 318
BpuMI CCSGG 2 cut(s) 22, 476
Bsa29I ATCGAT 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 199
BsaI GGTCTC 2 cut(s) 230, 278
BsaJI CCNNGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 338, 383
Bse1I ACTGG 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 337, 338, 383
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 1 cut(s) 240
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 2 cut(s) 94, 330
BshVI ATCGAT 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 193
BsiSI CCGG 2 cut(s) 21, 475
BsiWI CGTACG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 3 cut(s) 337, 338, 383
BsmAI GTCTC 3 cut(s) 190, 230, 278
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 2 cut(s) 94, 330
Bso31I GGTCTC 2 cut(s) 230, 278
BsoBI CYCGRG 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 358
Bsp143I GATC 5 cut(s) 30, 106, 220, 460, 524
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 2 cut(s) 164, 338
BspANI GGCC 2 cut(s) 94, 330
BspCNI CTCAG 1 cut(s) 274
BspDI ATCGAT 1 cut(s) 105
BspMI ACCTGC 1 cut(s) 18
BspPI GGATC 1 cut(s) 101
BspTNI GGTCTC 2 cut(s) 230, 278
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 5 cut(s) 30, 106, 220, 460, 524
BssNI GRCGYC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 349
BstACI GRCGYC 1 cut(s) 199
BstAUI TGTACA 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 261, 276
BstDSI CCRYGG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 381
BstF5I GGATG 1 cut(s) 318
BstKTI GATC 5 cut(s) 33, 109, 223, 463, 527
BstMAI GTCTC 3 cut(s) 190, 230, 278
BstMBI GATC 5 cut(s) 30, 106, 220, 460, 524
BstMWI GCNNNNNNNGC 3 cut(s) 10, 198, 509
BstNSI RCATGY 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 20, 474
BstV2I GAAGAC 2 cut(s) 32, 294
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
Bsu15I ATCGAT 1 cut(s) 105
BsuRI GGCC 2 cut(s) 94, 330
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 247
BveI ACCTGC 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 92, 329
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 2 cut(s) 207, 288
Csp6I GTAC 3 cut(s) 82, 97, 359
CviAII CATG 3 cut(s) 148, 179, 356
CviQI GTAC 3 cut(s) 82, 97, 359
DdeI CTNAG 2 cut(s) 261, 276
DpnI GATC 5 cut(s) 32, 108, 222, 462, 526
DpnII GATC 5 cut(s) 30, 106, 220, 460, 524
Eco130I CCWWGG 1 cut(s) 147
Eco31I GGTCTC 2 cut(s) 230, 278
Eco57I CTGAAG 1 cut(s) 467
Eco88I CYCGRG 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 3 cut(s) 151, 182, 359
FaiI YATR 7 cut(s) 144, 149, 180, 294, 357, 369, 417
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FaqI GGGAC 1 cut(s) 354
FatI CATG 3 cut(s) 147, 178, 355
FauI CCCGC 1 cut(s) 331
FblI GTMKAC 1 cut(s) 68
FokI GGATG 1 cut(s) 305
FspBI CTAG 3 cut(s) 39, 464, 541
HaeIII GGCC 2 cut(s) 94, 330
HapII CCGG 2 cut(s) 21, 475
HgaI GACGC 2 cut(s) 207, 288
Hin1I GRCGYC 1 cut(s) 199
Hin1II CATG 3 cut(s) 151, 182, 359
HindIII AAGCTT 2 cut(s) 278, 455
HpaII CCGG 2 cut(s) 21, 475
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 69, 361
Hpy188I TCNGA 2 cut(s) 54, 264
Hpy188III TCNNGA 3 cut(s) 425, 464, 494
Hpy8I GTNNAC 2 cut(s) 69, 361
HpyAV CCTTC 4 cut(s) 259, 298, 427, 433
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4V TGCA 2 cut(s) 13, 176
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 198, 509
HpyF3I CTNAG 2 cut(s) 261, 276
Hsp92I GRCGYC 1 cut(s) 199
Hsp92II CATG 3 cut(s) 151, 182, 359
Kzo9I GATC 5 cut(s) 30, 106, 220, 460, 524
LmnI GCTCC 1 cut(s) 486
LpnPI CCDG 7 cut(s) 23, 34, 186, 253, 260, 479, 488
LweI GCATC 1 cut(s) 409
MaeI CTAG 3 cut(s) 39, 464, 541
MaeIII GTNAC 3 cut(s) 16, 242, 389
MalI GATC 5 cut(s) 32, 108, 222, 462, 526
MboI GATC 5 cut(s) 30, 106, 220, 460, 524
MboII GAAGA 5 cut(s) 37, 69, 161, 294, 412
MflI RGATCY 1 cut(s) 524
MnlI CCTC 4 cut(s) 144, 342, 387, 502
MseI TTAA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 21, 475
MspR9I CCNGG 2 cut(s) 22, 476
Mva1269I GAATGC 1 cut(s) 489
MwoI GCNNNNNNNGC 3 cut(s) 10, 198, 509
NciI CCSGG 2 cut(s) 22, 476
NcoI CCATGG 1 cut(s) 147
NdeII GATC 5 cut(s) 30, 106, 220, 460, 524
NlaIII CATG 3 cut(s) 151, 182, 359
NmuCI GTSAC 2 cut(s) 242, 389
NspI RCATGY 1 cut(s) 182
PciI ACATGT 1 cut(s) 178
PctI GAATGC 1 cut(s) 489
Pfl23II CGTACG 1 cut(s) 81
PscI ACATGT 1 cut(s) 178
PspLI CGTACG 1 cut(s) 81
PspPI GGNCC 2 cut(s) 92, 329
PsuI RGATCY 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 3 cut(s) 83, 98, 360
RsaNI GTAC 3 cut(s) 82, 97, 359
SaqAI TTAA 1 cut(s) 153
Sau3AI GATC 5 cut(s) 30, 106, 220, 460, 524
Sau96I GGNCC 2 cut(s) 92, 329
ScrFI CCNGG 2 cut(s) 22, 476
SetI ASST 8 cut(s) 12, 232, 249, 270, 282, 290, 388, 459
SfaNI GCATC 1 cut(s) 409
SmlI CTYRAG 2 cut(s) 281, 333
SmoI CTYRAG 2 cut(s) 281, 333
SsiI CCGC 2 cut(s) 164, 338
SspMI CTAG 3 cut(s) 39, 464, 541
StyD4I CCNGG 2 cut(s) 20, 474
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 1 cut(s) 349
TaqI TCGA 2 cut(s) 105, 534
TatI WGTACW 1 cut(s) 358
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 2 cut(s) 242, 389
Tsp45I GTSAC 2 cut(s) 242, 389
TspDTI ATGAA 2 cut(s) 356, 423
TspGWI ACGGA 1 cut(s) 149
TspRI CASTG 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 381
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 68
XspI CTAG 3 cut(s) 39, 464, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.