MD11G1048500.v1.1

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003692
Physical Location & Seq
Reverse (-)
4219749 .. 4221381
1633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1048500.v1.1.491

Sequence Viewer

Length: 852 bp
ATGGTGTCAGCCAGTAATCATGTCCTTCGGTTGGCAAAGGAGCTTGAATATGTACTGATCAATACGTGGTTCACGATTTTCAAGCACACTTTTTACTTACACCTTGTAGTGACACATGGCAACAATTTTCTTCAGATCATCCGGAATCTATCACGAAGACTAAATCTTCCACTGGATGGAGAGGCAACCGTACAAACTACTGCTATTACGAGAAAAGTCTATCCAGTACCCAACCTACCAAAAAAGTTAATGCCAGCAAGGTTTGAAGCTTGGAAATTGTGGCATTTAGAGGGGATGTCGATGCAGAAAGTTGCCAACTTCCCTAGTAGATCAGCTCCTATTAAAGAGCAGACTGTTCTTGGTTATCTGGTACAAGCAGCTCAAGAAGGATGTGAAATTGATTGGATCAGGCTCTGCAACGAGGTCAGACTAACACATAAAGTTCTCTTGGATATTCAGTGTGCCATTTCAAAGGTTGGCTCTATGGAAAGGCTAAAGCCTATCAAAGACGAATTGCCAGAAGACATAAGTTATGCACACATCAATATTTGCCTAGCAATGCAAAAGCTTGGAGTGTCTCTGGAAGCTGGTCAACTTCCGAATAAGGAAACACAATCGTCACCGTGTTCTGCTCGTGCAACCCCCATGGAAGAACCTCTTGAAGATAAAAATGAAGAAACCGCTTCTCTTCCCTTGACCAGGAGACAAAGAGGAAAGCAACCCAAAGAACATTTAGAGGATTTACTTACTCCCAACGATTGGCTTAAGAACCAAGACGGGGTAAGTAGTGTAGTGAAATGGAAAGTGAAATGGAAAGCTTTATCATCCTCACCTTTCGACCCTGCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

284

Amino Acids

32.19

Weight (kDa)

8.87

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_40 PF14493 89 - 184 5e-17 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 176, 759
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 1 cut(s) 681
AclWI GGATC 1 cut(s) 413
AcuI CTGAAG 1 cut(s) 116
AfaI GTAC 4 cut(s) 54, 192, 228, 372
AfiI CCNNNNNNNGG 5 cut(s) 31, 176, 699, 759, 778
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 5 cut(s) 47, 82, 266, 471, 662
AjnI CCWGG 1 cut(s) 698
AluBI AGCT 8 cut(s) 43, 269, 335, 380, 568, 587, 818, 849
AluI AGCT 8 cut(s) 43, 269, 335, 380, 568, 587, 818, 849
Alw26I GTCTC 2 cut(s) 582, 697
AlwI GGATC 1 cut(s) 413
AlwNI CAGNNNCTG 1 cut(s) 414
Aor13HI TCCGGA 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 377
AsuHPI GGTGA 2 cut(s) 612, 822
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 2 cut(s) 163, 528
BbvI GCAGC 1 cut(s) 389
BccI CCATC 1 cut(s) 170
BciT130I CCWGG 1 cut(s) 700
BclI TGATCA 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 582, 697
BfaI CTAG 2 cut(s) 324, 554
BfrI CTTAAG 1 cut(s) 764
BisI GCNGC 1 cut(s) 378
BlsI GCNGC 1 cut(s) 379
Bme1390I CCNGG 1 cut(s) 700
BmrFI CCNGG 1 cut(s) 700
BmsI GCATC 1 cut(s) 291
BpiI GAAGAC 2 cut(s) 163, 528
BpuEI CTTGAG 1 cut(s) 366
BsaAI YACGTR 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 645
BsaWI WCCGGW 1 cut(s) 141
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 176, 699, 759, 778
Bse1I ACTGG 3 cut(s) 12, 177, 224
Bse3DI GCAATG 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 1 cut(s) 700
BseDI CCNNGG 1 cut(s) 645
BseGI GGATG 5 cut(s) 138, 181, 300, 395, 824
BseLI CCNNNNNNNGG 5 cut(s) 31, 176, 699, 759, 778
BseMI GCAATG 1 cut(s) 564
BseNI ACTGG 3 cut(s) 12, 177, 224
BseXI GCAGC 1 cut(s) 389
BsiSI CCGG 1 cut(s) 142
BslI CCNNNNNNNGG 5 cut(s) 31, 176, 699, 759, 778
BsmAI GTCTC 2 cut(s) 582, 697
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 57, 135, 329, 405
Bsp19I CCATGG 1 cut(s) 645
BspACI CCGC 1 cut(s) 681
BspEI TCCGGA 1 cut(s) 141
BspPI GGATC 1 cut(s) 413
BspTI CTTAAG 1 cut(s) 764
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 3 cut(s) 12, 177, 224
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 4 cut(s) 57, 135, 329, 405
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 1 cut(s) 645
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 1 cut(s) 700
Bst4CI ACNGT 3 cut(s) 190, 355, 624
Bst6I CTCTTC 1 cut(s) 693
BstAFI CTTAAG 1 cut(s) 764
BstBAI YACGTR 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 255, 847
BstDSI CCRYGG 1 cut(s) 645
BstENI CCTNNNNNAGG 1 cut(s) 697
BstF5I GGATG 5 cut(s) 138, 181, 300, 395, 824
BstKTI GATC 4 cut(s) 60, 138, 332, 408
BstMAI GTCTC 2 cut(s) 582, 697
BstMBI GATC 4 cut(s) 57, 135, 329, 405
BstNI CCWGG 1 cut(s) 700
BstSCI CCNGG 1 cut(s) 698
BstV1I GCAGC 1 cut(s) 389
BstV2I GAAGAC 2 cut(s) 163, 528
BtgI CCRYGG 1 cut(s) 645
BtsCI GGATG 5 cut(s) 138, 181, 300, 395, 824
BtsIMutI CAGTG 2 cut(s) 170, 464
Cac8I GCNNGC 2 cut(s) 255, 847
CaiI CAGNNNCTG 1 cut(s) 414
Csp6I GTAC 4 cut(s) 53, 191, 227, 371
CviAII CATG 3 cut(s) 20, 116, 646
CviQI GTAC 4 cut(s) 53, 191, 227, 371
DpnI GATC 4 cut(s) 59, 137, 331, 407
DpnII GATC 4 cut(s) 57, 135, 329, 405
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco130I CCWWGG 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 116
EcoNI CCTNNNNNAGG 1 cut(s) 697
EcoRII CCWGG 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 3 cut(s) 23, 119, 649
FaiI YATR 8 cut(s) 21, 51, 117, 438, 485, 527, 534, 647
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FatI CATG 3 cut(s) 19, 115, 645
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 1 cut(s) 378
FokI GGATG 5 cut(s) 125, 188, 307, 402, 811
Fsp4HI GCNGC 1 cut(s) 378
FspBI CTAG 2 cut(s) 324, 554
GluI GCNGC 1 cut(s) 378
HapII CCGG 1 cut(s) 142
Hin1II CATG 3 cut(s) 23, 119, 649
HincII GTYRAC 1 cut(s) 593
HindII GTYRAC 1 cut(s) 593
HindIII AAGCTT 3 cut(s) 267, 566, 816
HinfI GANTC 1 cut(s) 145
HpaII CCGG 1 cut(s) 142
HphI GGTGA 2 cut(s) 612, 822
Hpy166II GTNNAC 2 cut(s) 72, 593
Hpy188I TCNGA 3 cut(s) 135, 428, 600
Hpy188III TCNNGA 6 cut(s) 73, 142, 153, 383, 581, 659
Hpy8I GTNNAC 2 cut(s) 72, 593
HpyAV CCTTC 2 cut(s) 35, 380
HpyCH4III ACNGT 3 cut(s) 190, 355, 624
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 6 cut(s) 304, 417, 536, 562, 638, 845
HpySE526I ACGT 1 cut(s) 65
Hsp92II CATG 3 cut(s) 23, 119, 649
Kpn2I TCCGGA 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 4 cut(s) 57, 135, 329, 405
LmnI GCTCC 2 cut(s) 40, 340
Lsp1109I GCAGC 1 cut(s) 389
LweI GCATC 1 cut(s) 291
MaeI CTAG 2 cut(s) 324, 554
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 2 cut(s) 109, 618
MalI GATC 4 cut(s) 59, 137, 331, 407
MboI GATC 4 cut(s) 57, 135, 329, 405
MboII GAAGA 8 cut(s) 122, 158, 168, 533, 662, 674, 680, 686
MluCI AATT 4 cut(s) 124, 275, 396, 512
MnlI CCTC 7 cut(s) 175, 283, 415, 666, 704, 730, 838
MroI TCCGGA 1 cut(s) 141
MseI TTAA 3 cut(s) 248, 342, 765
MspCI CTTAAG 1 cut(s) 764
MspI CCGG 1 cut(s) 142
MspR9I CCNGG 1 cut(s) 700
MvaI CCWGG 1 cut(s) 700
NcoI CCATGG 1 cut(s) 645
NdeII GATC 4 cut(s) 57, 135, 329, 405
NlaIII CATG 3 cut(s) 23, 119, 649
NmuCI GTSAC 2 cut(s) 109, 618
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PfeI GAWTC 1 cut(s) 145
PflMI CCANNNNNTGG 2 cut(s) 176, 759
PkrI GCNGC 1 cut(s) 379
Ppu21I YACGTR 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 698
PspGI CCWGG 1 cut(s) 698
PstNI CAGNNNCTG 1 cut(s) 414
RsaI GTAC 4 cut(s) 54, 192, 228, 372
RsaNI GTAC 4 cut(s) 53, 191, 227, 371
SaqAI TTAA 3 cut(s) 248, 342, 765
SatI GCNGC 1 cut(s) 378
Sau3AI GATC 4 cut(s) 57, 135, 329, 405
ScrFI CCNGG 1 cut(s) 700
SfaNI GCATC 1 cut(s) 291
SmlI CTYRAG 2 cut(s) 381, 764
SmoI CTYRAG 2 cut(s) 381, 764
Sse9I AATT 4 cut(s) 124, 275, 396, 512
SsiI CCGC 1 cut(s) 681
SspI AATATT 1 cut(s) 547
SspMI CTAG 2 cut(s) 324, 554
StyD4I CCNGG 1 cut(s) 698
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 3 cut(s) 190, 355, 624
TaiI ACGT 1 cut(s) 68
TaqI TCGA 2 cut(s) 299, 837
TasI AATT 4 cut(s) 124, 275, 396, 512
TatI WGTACW 1 cut(s) 52
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 3 cut(s) 248, 342, 765
Tru9I TTAA 3 cut(s) 248, 342, 765
TscAI CASTG 2 cut(s) 177, 464
TseFI GTSAC 2 cut(s) 109, 618
TseI GCWGC 1 cut(s) 377
Tsp45I GTSAC 2 cut(s) 109, 618
TspDTI ATGAA 1 cut(s) 687
TspRI CASTG 2 cut(s) 177, 464
Van91I CCANNNNNTGG 2 cut(s) 176, 759
Vha464I CTTAAG 1 cut(s) 764
XagI CCTNNNNNAGG 1 cut(s) 697
XspI CTAG 2 cut(s) 324, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.