RchiOBHm_Chr5g0074061

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003692
Physical Location & Seq
Forward (+)
80091594 .. 80099709
8116 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34890

Sequence Viewer

Length: 2754 bp
ATGCAGTCTATGCAGGTCATTCTCAAGCAATATTTTGGGTTTTCTTCATTTCGGCCATACCAAGAGGAAGTGATTGAGAAAATCATAGCAGGAAGGGACTCTTTGATTGTCATGGCCACTGGAAGTGGCAAGTCCTTGTGCTATCAAGTGCCTCCTTTGGTTGTTGGAAAGACTGGTGTGGTTGTGAGCCCTCTTATATCCTTAATGCAAGATCAGGTGATGTCTTTGAAACAAAGAGGGATCAGAGCCGAGTATATGGGTAGTAGTCAATCGGATAACACTGTCCAGAGCCGAGCCGAGAGTGGTCAATTTGATATCTTGTACATGACCCCTGAAAAGGCATGTGTGATTCCTGTCAGCTTCTGGTCAAAATTACTAAGGGCTGGGATCTGCTTGTTTGCTGTTGATGAAGCACACTGCATATCGGAGTGGGGCCATGATTTCAGGGTGGAATACAAGCAATTGGACAAGTTACGTGGCGTTCTTGTTGGTGTCCCATTTATTGGCTTAACTGCAACTGCTACTGAAAAGGTTCGAATGGACGTTGTTAATTCCTTGAAGATGGAAAACCCATATGTGAAAATAGGCTCATTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTAATGAGCTGGTTCAAGAAGTTTCCAAATTTGTACGCACAGATGGTTCAACAATAATATACTGCACAACAATTAAAGATGTTGATCAGGTATTCAATTCACTGAAGGAGGTGGGCATTAAAGCTGGAATCTACCATGGTCAAATGGATAATAAAGCTCGTGCCGAATCCCATAGATTGTTTATAAGAGATGAACTGGATGTCATGGTTGCCACTATTGCTTTTGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCACTATGGCTGCCCGAAGAGTTTGGAGTCTTATTACCAGGAAAGTGGACGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACTTCACAAAAGCTGACTTTTATGCTGGAGAGTGTCAAACAGAAAGCCAACGAAGAGCTGTTGTAGAGTCATTGATGGCAGCACAACAGTATTGCTTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTCACTTTGGGGAAATCTTTCCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCTTCAAAGAAGGAGAGGGACATGTCTAGAGAAGCATTTCTTCTAATGGCTTGCATTCAATCATGTCGGGGTAAATGGGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCTCGGGCGAAAAAGATTCTTGATGCCCAATATGACAAGCTTCCACTGCATGGACTTGGGAAAGAGTATTCAGCAAATTGGTGGAAAGCACTTGCTTACCAATTAATCTCTTCTGGTTATTTGACGGAGACGGTATCTGATATATACAGGACTGTAAGTGTCAGTCTGAAAGGGGAGCAATTTCTAAGTTCTGCTGGACCTGATCATCAACCGCCTCTAGTTTTGCCAGTGACCAGTGAAATGGTAGATGATGAGGACAATAAAAGCACATCAGGTGAAGTTGGAGAAATCAAAAGTTTGTCTACTCTGGAATGCGAAGGATTTTCGGAGGATGAGAAGCAACTGTATCACTCACTTCTTGAAGAGAGAAGGAAGCTTGCAAGAAGCCTTGGAACTGCTCCATATGCTATTTGTGGTGATCAAGCAATCAAAAAAATTGCTTTAACAAGACCATCTACCAGAGCAAGGTTAGCTAACATCGATGGCGTCAACCAGCACCTTGTAGTGGCACACGGTAATCATTTTCTTCAAATCATTCGGCATCTATCACAAGGACTAAACCTTTTGTTGGATGGAGAGGCACCCATACAAACTGCTATTACAAAGAAAGTATATCCAGTATCTAACCACCAACAGAGAAAGTTAACGCCGGCAAAGTTTGAAGCTTGGAGAATGTGGCATGAAGACAGCCTCTCAATTCAGAAAATTGCTAATTTCCCTGGTAGAGCTGCTCCTATTAAAGAGCAGACTGTTCAAGAATATCTGATGGAAGCTGCTCAAGAAGGATTTGCAATTGATTGGGTCAGATTATGCGATGAGGTTGGACTGACAGAAAAAGTTATCTCAGATATTCAGTGTGCCATTTCAAAGGTTGGCTCTAGAGAAAAGTTAAAGCCTATCAAGGACGAATTACAAGAAGATATAAGTTATGGACACATCAAGACTTTCCTGACAATGGAAAAGTGTGGGATCTCGCTGGATGGGCCTTTACCTAACCACCACAATGCAGGAAAAGATGATCAACTCCTCAAGAAGGAAACTGAATTGTTACCGAGTTCTATGCATACATCCCCTATGGAAGAACCTCATGAAGTTAAAACCTCAGGCCGAGATTCAGATGCTTACAGCTTGGATAAAAATAAAGAAACAGCTTCCCTTCCATTCAATAGAGAACAAGGTTTAAAGCTACCTGAAGTACATTTTGAGGATTTACTCTCAAAGAAACGCCAAAAGCTTGGTAGTCCCAAGGAGGAGAGTAAAACTACACTGAAGGCAACTGAGAATTCCATATTGGATTGGCTTAGTACCAAAAATGGAGTTACTCTCTCTGCGATTCTGGAGCACTTCAATGGATCTGAAGAAGACTGTGTCATCGAACGCCTCACTGGCCTTGAAAGTGACTTTATGATATACAGAAAGAACAATATGTATTTCGTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

917

Amino Acids

103.09

Weight (kDa)

6.55

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 16 - 173 6.4e-06 Type III restriction enzyme, res subunit
DEAD PF00270 18 - 179 2.2e-24 DEAD/DEAH box helicase
Helicase_C PF00271 224 - 326 1.2e-21 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 338 - 399 4.8e-14 RecQ zinc-binding
RQC PF09382 403 - 500 8.9e-24 RQC domain
HRDC PF00570 550 - 616 1.1e-13 HRDC domain
HTH_40 PF14493 658 - 753 2e-17 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
Acc36I ACCTGC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 1891
AccB7I CCANNNNNTGG 2 cut(s) 503, 1361
AccI GTMKAC 1 cut(s) 1613
AciI CCGC 1 cut(s) 1523
AclWI GGATC 4 cut(s) 248, 395, 2288, 2671
AcoI YGGCCR 2 cut(s) 53, 114
AcsI RAATTY 2 cut(s) 677, 2593
AcuI CTGAAG 4 cut(s) 773, 2523, 2600, 2688
AcyI GRCGYC 1 cut(s) 1797
AfaI GTAC 4 cut(s) 323, 684, 2508, 2617
AfiI CCNNNNNNNGG 8 cut(s) 337, 503, 1361, 1776, 1816, 1879, 2266, 2344
AflIII ACRYGT 1 cut(s) 1221
AjnI CCWGG 2 cut(s) 948, 2029
AjuI GAANNNNNNNTTGG 2 cut(s) 2585, 2617
Alw21I GWGCWC 1 cut(s) 2655
Alw26I GTCTC 1 cut(s) 1433
AlwI GGATC 4 cut(s) 248, 395, 2288, 2671
AlwNI CAGNNNCTG 1 cut(s) 363
Ama87I CYCGRG 1 cut(s) 1314
AoxI GGCC 6 cut(s) 53, 114, 433, 2294, 2416, 2698
ApeKI GCWGC 4 cut(s) 921, 1094, 2039, 2084
ApoI RAATTY 2 cut(s) 677, 2593
AseI ATTAAT 1 cut(s) 1415
Asp700I GAANNNNTTC 3 cut(s) 531, 1161, 1236
AspS9I GGNCC 3 cut(s) 433, 1508, 2294
AsuHPI GGTGA 3 cut(s) 229, 1598, 1739
AsuII TTCGAA 1 cut(s) 535
AvaI CYCGRG 1 cut(s) 1314
AvaII GGWCC 1 cut(s) 1508
AxyI CCTNAGG 1 cut(s) 2413
BalI TGGCCA 1 cut(s) 116
BanI GGYRCC 1 cut(s) 1891
BanII GRGCYC 1 cut(s) 191
BarI GAAGNNNNNNTAC 2 cut(s) 1287, 1319
BauI CACGAG 1 cut(s) 807
BbsI GAAGAC 2 cut(s) 2001, 2679
Bbv12I GWGCWC 1 cut(s) 2655
BbvI GCAGC 4 cut(s) 908, 1106, 2026, 2071
BcgI CGANNNNNNTGC 2 cut(s) 2353, 2387
BciT130I CCWGG 2 cut(s) 950, 2031
BclI TGATCA 4 cut(s) 733, 1513, 1729, 2329
BcoDI GTCTC 1 cut(s) 1433
BfaI CTAG 3 cut(s) 1227, 1529, 2190
BfmI CTRYAG 1 cut(s) 1293
BfuAI ACCTGC 1 cut(s) 4
BglI GCCNNNNNGGC 1 cut(s) 2697
BisI GCNGC 4 cut(s) 922, 1095, 2040, 2085
BlsI GCNGC 4 cut(s) 923, 1096, 2041, 2086
Bme1390I CCNGG 2 cut(s) 950, 2031
Bme18I GGWCC 1 cut(s) 1508
BmeT110I CYCGRG 1 cut(s) 1314
BmgT120I GGNCC 3 cut(s) 433, 1508, 2294
BmiI GGNNCC 2 cut(s) 434, 1893
BmrFI CCNGG 2 cut(s) 950, 2031
BmsI GCATC 3 cut(s) 1324, 1861, 2419
BpiI GAAGAC 2 cut(s) 2001, 2679
BplI GAGNNNNNCTC 2 cut(s) 2619, 2651
BpmI CTGGAG 2 cut(s) 1062, 2669
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 3 cut(s) 8, 2073, 2324
Bsa29I ATCGAT 1 cut(s) 1791
BsaAI YACGTR 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 1797
BsaJI CCNNGG 4 cut(s) 784, 1699, 2029, 2556
Bsc4I CCNNNNNNNGG 8 cut(s) 337, 503, 1361, 1776, 1816, 1879, 2266, 2344
Bse118I RCCGGY 1 cut(s) 1960
Bse1I ACTGG 7 cut(s) 124, 178, 849, 1538, 1545, 1928, 2701
Bse21I CCTNAGG 1 cut(s) 2413
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 950, 2031
BseCI ATCGAT 1 cut(s) 1791
BseDI CCNNGG 4 cut(s) 784, 1699, 2029, 2556
BseGI GGATG 5 cut(s) 853, 1648, 1888, 2296, 2378
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 8 cut(s) 337, 503, 1361, 1776, 1816, 1879, 2266, 2344
BseMII CTCAG 3 cut(s) 2169, 2427, 2580
BseNI ACTGG 7 cut(s) 124, 178, 849, 1538, 1545, 1928, 2701
BseRI GAGGAG 2 cut(s) 2327, 2576
BseXI GCAGC 4 cut(s) 908, 1106, 2026, 2071
BseYI CCCAGC 1 cut(s) 383
BsgI GTGCAG 1 cut(s) 697
BshFI GGCC 6 cut(s) 55, 116, 435, 2296, 2418, 2700
BshNI GGYRCC 1 cut(s) 1891
BshVI ATCGAT 1 cut(s) 1791
BsiHKAI GWGCWC 1 cut(s) 2655
BsiHKCI CYCGRG 1 cut(s) 1314
BsiSI CCGG 1 cut(s) 1961
BslFI GGGAC 4 cut(s) 110, 479, 1232, 2538
BslI CCNNNNNNNGG 8 cut(s) 337, 503, 1361, 1776, 1816, 1879, 2266, 2344
BsmAI GTCTC 1 cut(s) 1433
BsmBI CGTCTC 1 cut(s) 1433
BsmFI GGGAC 4 cut(s) 110, 479, 1232, 2538
BsmI GAATGC 2 cut(s) 1254, 1628
BsnI GGCC 6 cut(s) 55, 116, 435, 2296, 2418, 2700
BsoBI CYCGRG 1 cut(s) 1314
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 2 cut(s) 191, 2655
Bsp1407I TGTACA 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 784
BspACI CCGC 1 cut(s) 1523
BspANI GGCC 6 cut(s) 55, 116, 435, 2296, 2418, 2700
BspCNI CTCAG 3 cut(s) 2168, 2426, 2581
BspDI ATCGAT 1 cut(s) 1791
BspHI TCATGA 1 cut(s) 2398
BspLI GGNNCC 2 cut(s) 434, 1893
BspMI ACCTGC 1 cut(s) 4
BspPI GGATC 4 cut(s) 248, 395, 2288, 2671
BspQI GCTCTTC 1 cut(s) 1063
BspT104I TTCGAA 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 1891
BsrFI RCCGGY 1 cut(s) 1960
BsrGI TGTACA 1 cut(s) 321
BsrI ACTGG 7 cut(s) 124, 178, 849, 1538, 1545, 1928, 2701
BssAI RCCGGY 1 cut(s) 1960
BssECI CCNNGG 4 cut(s) 784, 1699, 2029, 2556
BssNI GRCGYC 1 cut(s) 1797
BssSI CACGAG 1 cut(s) 807
BssT1I CCWWGG 3 cut(s) 784, 1699, 2556
Bst2BI CACGAG 1 cut(s) 807
Bst2UI CCWGG 2 cut(s) 950, 2031
Bst4CI ACNGT 8 cut(s) 283, 1104, 1444, 1465, 1656, 1826, 2062, 2678
Bst6I CTCTTC 4 cut(s) 923, 1063, 1426, 1668
BstACI GRCGYC 1 cut(s) 1797
BstAPI GCANNNNNTGC 1 cut(s) 10
BstAUI TGTACA 1 cut(s) 321
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 4 cut(s) 995, 1252, 1689, 1962
BstDEI CTNAG 6 cut(s) 377, 1496, 2155, 2413, 2589, 2612
BstDSI CCRYGG 1 cut(s) 784
BstENI CCTNNNNNAGG 1 cut(s) 2342
BstF5I GGATG 5 cut(s) 853, 1648, 1888, 2296, 2378
BstMAI GTCTC 1 cut(s) 1433
BstMWI GCNNNNNNNGC 7 cut(s) 10, 866, 1357, 1715, 1781, 2293, 2697
BstNI CCWGG 2 cut(s) 950, 2031
BstNSI RCATGY 2 cut(s) 345, 1225
BstSCI CCNGG 2 cut(s) 948, 2029
BstSFI CTRYAG 1 cut(s) 1293
BstV1I GCAGC 4 cut(s) 908, 1106, 2026, 2071
BstV2I GAAGAC 2 cut(s) 2001, 2679
BstX2I RGATCY 3 cut(s) 387, 2280, 2663
BstXI CCANNNNNNTGG 3 cut(s) 956, 1552, 2546
BstYI RGATCY 3 cut(s) 387, 2280, 2663
Bsu15I ATCGAT 1 cut(s) 1791
Bsu36I CCTNAGG 1 cut(s) 2413
BsuRI GGCC 6 cut(s) 55, 116, 435, 2296, 2418, 2700
BsuTUI ATCGAT 1 cut(s) 1791
BtgI CCRYGG 1 cut(s) 784
BtgZI GCGATG 1 cut(s) 2139
BtsCI GGATG 5 cut(s) 853, 1648, 1888, 2296, 2378
BtsI GCAGTG 2 cut(s) 415, 1355
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 4 cut(s) 995, 1252, 1689, 1962
CaiI CAGNNNCTG 1 cut(s) 363
CciI TCATGA 1 cut(s) 2398
Cfr10I RCCGGY 1 cut(s) 1960
Cfr13I GGNCC 3 cut(s) 433, 1508, 2294
ClaI ATCGAT 1 cut(s) 1791
CseI GACGC 1 cut(s) 1786
Csp6I GTAC 4 cut(s) 322, 683, 2507, 2616
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviQI GTAC 4 cut(s) 322, 683, 2507, 2616
DdeI CTNAG 6 cut(s) 377, 1496, 2155, 2413, 2589, 2612
DraI TTTAAA 1 cut(s) 2493
EaeI YGGCCR 2 cut(s) 53, 114
Eam1104I CTCTTC 4 cut(s) 923, 1063, 1426, 1668
EarI CTCTTC 4 cut(s) 923, 1063, 1426, 1668
Eco130I CCWWGG 3 cut(s) 784, 1699, 2556
Eco24I GRGCYC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 1508
Eco57I CTGAAG 4 cut(s) 773, 2523, 2600, 2688
Eco81I CCTNAGG 1 cut(s) 2413
Eco88I CYCGRG 1 cut(s) 1314
EcoNI CCTNNNNNAGG 1 cut(s) 2342
EcoRI GAATTC 1 cut(s) 2593
EcoRII CCWGG 2 cut(s) 948, 2029
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 784, 1699, 2556
EcoT22I ATGCAT 1 cut(s) 2376
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 784, 1699, 2556
Esp3I CGTCTC 1 cut(s) 1433
FalI AAGNNNNNCTT 4 cut(s) 85, 117, 1224, 1256
FaqI GGGAC 4 cut(s) 110, 479, 1232, 2538
FauNDI CATATG 2 cut(s) 574, 1714
FbaI TGATCA 4 cut(s) 733, 1513, 1729, 2329
FblI GTMKAC 1 cut(s) 1613
Fnu4HI GCNGC 4 cut(s) 922, 1095, 2040, 2085
FokI GGATG 5 cut(s) 860, 1655, 1895, 2303, 2365
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 4 cut(s) 922, 1095, 2040, 2085
FspBI CTAG 3 cut(s) 1227, 1529, 2190
GluI GCNGC 4 cut(s) 922, 1095, 2040, 2085
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 2 cut(s) 1062, 2669
HaeIII GGCC 6 cut(s) 55, 116, 435, 2296, 2418, 2700
HapII CCGG 1 cut(s) 1961
HgaI GACGC 1 cut(s) 1786
Hin1I GRCGYC 1 cut(s) 1797
HincII GTYRAC 2 cut(s) 1801, 1956
HindII GTYRAC 2 cut(s) 1801, 1956
HindIII AAGCTT 4 cut(s) 1349, 1685, 1974, 2543
HpaI GTTAAC 1 cut(s) 1956
HpaII CCGG 1 cut(s) 1961
HphI GGTGA 3 cut(s) 229, 1598, 1739
Hpy166II GTNNAC 4 cut(s) 959, 1614, 1801, 1956
Hpy8I GTNNAC 4 cut(s) 959, 1614, 1801, 1956
HpyCH4III ACNGT 8 cut(s) 283, 1104, 1444, 1465, 1656, 1826, 2062, 2678
HpyCH4IV ACGT 2 cut(s) 475, 543
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 866, 1357, 1715, 1781, 2293, 2697
HpyF3I CTNAG 6 cut(s) 377, 1496, 2155, 2413, 2589, 2612
HpySE526I ACGT 2 cut(s) 475, 543
Hsp92I GRCGYC 1 cut(s) 1797
KroI GCCGGC 1 cut(s) 1960
KroNI GCCGGC 1 cut(s) 1962
Ksp22I TGATCA 4 cut(s) 733, 1513, 1729, 2329
KspAI GTTAAC 1 cut(s) 1956
LguI GCTCTTC 1 cut(s) 1063
LmnI GCTCC 4 cut(s) 1486, 1714, 2047, 2650
Lsp1109I GCAGC 4 cut(s) 908, 1106, 2026, 2071
LweI GCATC 3 cut(s) 1324, 1861, 2419
MaeI CTAG 3 cut(s) 1227, 1529, 2190
MaeII ACGT 2 cut(s) 475, 543
MaeIII GTNAC 6 cut(s) 471, 1145, 1540, 2358, 2629, 2708
MfeI CAATTG 2 cut(s) 461, 2103
MflI RGATCY 3 cut(s) 387, 2280, 2663
MhlI GDGCHC 2 cut(s) 191, 2655
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 4 cut(s) 92, 631, 947, 1091
MmeI TCCRAC 4 cut(s) 145, 1573, 1860, 2113
Mox20I TGGCCA 1 cut(s) 116
Mph1103I ATGCAT 1 cut(s) 2376
MroNI GCCGGC 1 cut(s) 1960
MroXI GAANNNNTTC 3 cut(s) 531, 1161, 1236
MscI TGGCCA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 2313, 2658
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 1 cut(s) 1961
MspR9I CCNGG 2 cut(s) 950, 2031
MunI CAATTG 2 cut(s) 461, 2103
Mva1269I GAATGC 2 cut(s) 1254, 1628
MvaI CCWGG 2 cut(s) 950, 2031
MwoI GCNNNNNNNGC 7 cut(s) 10, 866, 1357, 1715, 1781, 2293, 2697
NaeI GCCGGC 1 cut(s) 1962
NcoI CCATGG 1 cut(s) 784
NdeI CATATG 2 cut(s) 574, 1714
NgoMIV GCCGGC 1 cut(s) 1960
NlaIV GGNNCC 2 cut(s) 434, 1893
NmeAIII GCCGAG 4 cut(s) 274, 317, 322, 2444
NmuCI GTSAC 3 cut(s) 1145, 1540, 2708
NsiI ATGCAT 1 cut(s) 2376
NspI RCATGY 2 cut(s) 345, 1225
NspV TTCGAA 1 cut(s) 535
PagI TCATGA 1 cut(s) 2398
PciI ACATGT 1 cut(s) 1221
PciSI GCTCTTC 1 cut(s) 1063
PctI GAATGC 2 cut(s) 1254, 1628
PdiI GCCGGC 1 cut(s) 1962
PdmI GAANNNNTTC 3 cut(s) 531, 1161, 1236
PfeI GAWTC 6 cut(s) 349, 777, 815, 1327, 2423, 2644
PflFI GACNNNGTC 1 cut(s) 2678
PflMI CCANNNNNTGG 2 cut(s) 503, 1361
PkrI GCNGC 4 cut(s) 923, 1096, 2041, 2086
PleI GAGTC 4 cut(s) 92, 631, 946, 1090
PpsI GAGTC 4 cut(s) 92, 631, 946, 1090
Ppu21I YACGTR 1 cut(s) 476
PscI ACATGT 1 cut(s) 1221
PshBI ATTAAT 1 cut(s) 1415
PsiI TTATAA 1 cut(s) 833
Psp6I CCWGG 2 cut(s) 948, 2029
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 2 cut(s) 948, 2029
PspN4I GGNNCC 2 cut(s) 434, 1893
PspPI GGNCC 3 cut(s) 433, 1508, 2294
PstNI CAGNNNCTG 1 cut(s) 363
PsuI RGATCY 3 cut(s) 387, 2280, 2663
PsyI GACNNNGTC 1 cut(s) 2678
RsaI GTAC 4 cut(s) 323, 684, 2508, 2617
RsaNI GTAC 4 cut(s) 322, 683, 2507, 2616
RseI CAYNNNNRTG 2 cut(s) 2313, 2658
SapI GCTCTTC 1 cut(s) 1063
SatI GCNGC 4 cut(s) 922, 1095, 2040, 2085
Sau96I GGNCC 3 cut(s) 433, 1508, 2294
SchI GAGTC 4 cut(s) 92, 631, 947, 1091
ScrFI CCNGG 2 cut(s) 950, 2031
SduI GDGCHC 2 cut(s) 191, 2655
SfaNI GCATC 3 cut(s) 1324, 1861, 2419
SfcI CTRYAG 1 cut(s) 1293
SfuI TTCGAA 1 cut(s) 535
SinI GGWCC 1 cut(s) 1508
SmiMI CAYNNNNRTG 2 cut(s) 2313, 2658
SmlI CTYRAG 3 cut(s) 23, 2088, 2339
SmoI CTYRAG 3 cut(s) 23, 2088, 2339
SsiI CCGC 1 cut(s) 1523
SspI AATATT 1 cut(s) 32
SspMI CTAG 3 cut(s) 1227, 1529, 2190
StyD4I CCNGG 2 cut(s) 948, 2029
StyI CCWWGG 3 cut(s) 784, 1699, 2556
TaaI ACNGT 8 cut(s) 283, 1104, 1444, 1465, 1656, 1826, 2062, 2678
TaiI ACGT 2 cut(s) 478, 546
TaqI TCGA 3 cut(s) 535, 1791, 2685
TatI WGTACW 2 cut(s) 321, 2506
TfiI GAWTC 6 cut(s) 349, 777, 815, 1327, 2423, 2644
TseFI GTSAC 3 cut(s) 1145, 1540, 2708
TseI GCWGC 4 cut(s) 921, 1094, 2039, 2084
Tsp45I GTSAC 3 cut(s) 1145, 1540, 2708
TspDTI ATGAA 5 cut(s) 36, 423, 855, 2007, 2415
TspGWI ACGGA 1 cut(s) 1451
Tth111I GACNNNGTC 1 cut(s) 2678
Van91I CCANNNNNTGG 2 cut(s) 503, 1361
VpaK11BI GGWCC 1 cut(s) 1508
VspI ATTAAT 1 cut(s) 1415
XagI CCTNNNNNAGG 1 cut(s) 2342
XapI RAATTY 2 cut(s) 677, 2593
XbaI TCTAGA 2 cut(s) 1226, 2189
XceI RCATGY 2 cut(s) 345, 1225
XmiI GTMKAC 1 cut(s) 1613
XmnI GAANNNNTTC 3 cut(s) 531, 1161, 1236
XspI CTAG 3 cut(s) 1227, 1529, 2190
Zsp2I ATGCAT 1 cut(s) 2376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.