Rh5AG486500

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003692
Physical Location & Seq
Forward (+)
83047096 .. 83055071
7976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG486500.1

Sequence Viewer

Length: 2763 bp
ATGCAGTCTATGCAGGCCATTCTCAAGCAATATTTTGGGTTTTCTTCATTTCGGCCATACCAAGAGGAAGTGATTGAGAAAATCATAGCAGGAAGGGACTCTTTGATTGTCATGGCCACTGGAAGTGGCAAGTCCTTGTGCTATCAAGTGCCTCCTTTGGTTGTTGGAAAGACTGGTGTGGTTGTGAGCCCTCTTATATCCTTAATGCAAGATCAGGTAATGTCTTTGAAACAAAGAGGGATCAGAGCCGAGTATATGGGTAGTAGTCAATTGGATAACACTGTCCAGAGCCGAGCCGAGAGTGGTCAATTTGATATCTTGTACATGACACCTGAAAAGGCATGTGTGATTCCTGTCAGCTTCTGGTCAAAATTACTAAGGGCTGGGATCTGCTTGTTTGCTGTTGATGAAGCACACTGCATATCGGAGTGGGGCCATGATTTCAGGGTGGAATACAAGCAATTGGACAAGTTACGTGGCGTTCTTGTTGGTGTCCCATTTATTGGCTTAACTGCAACTGCTACTGAAAAAGTTCGAATGGACGTTGTTAATTCCTTGAAGATGGAAAACCCATATGTCAAAATAGGCTCATTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTCATGAGCTGGTTCAAGAAGTTTCCAAATTTGTACGCACTGATGGTTCAACAATAATATACTGCACGACAATTAAAGATGTTGATCAGGTATTCAATTCACTGAAGGAGGTGGGCATTAAAGCTGGAATCTACCATGGTCAAATGGATAATAAAGCTCGTGCCGAATCCCATAGATTGTTTATAAGAGATGAACTGGATGTCATGGTTGCCACTATTGCTTTTGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCACTATGGCTGCCCGAAGAGTTTGGAATCTTATTACCAGGAAAGTGGACGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACTTCACAAAAGCTGACTTTTATGCTGGAGAGTGTCAAACAGAAAGCCAACGAAGAGCTGTTGTAGAGTCATTGATGGCAGCACAACAGTATTGCTTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTCACTTTGGGGAAATCTTTCCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCTTCAAAGAAGGAGAGGGACATGTCTAGAGAAGCATTTCTTCTAATGGCTTGCATTCAATCATGTCGGGGTAAATGGGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCTCGGGCGAAAAAGATTCTTGATGCCCAATATGACAAGCTTCCACTGCATGGACTTGGGAAAGAGTATTCAGCAAATTGGTGGAAAGCACTTGCTTACCAATTAATCTCTTCTGGTTATTTGACGGAGACAGTATCTGATATATACAGGACTGTAAGTGTCAGTCTGAAAGGGGAGCAATTTCTAAGTTCTGCTGGACCTGATCATCAACCGCCTCTAGTTTTGCCAGTGACCAGTGAAATGGTAGATGATGAGGACAATAAAAGTACATCAGGTGAAGTTGGAGAAATCAAAAGTTTGTCTACTCTGGAATGTGAAGGATTTTCGGAGGCTGAGAAGCAAGTCAAGCAACTGTATCACTTACTTCTTGAAGAGAGAAGGAAGCTTGCAAGAAGCCTTGGAACTGCTCCATATGCTATTTGTGGTGATCAAGCAATCAAAAAAATTGCTTTAACAAGACCATCTACCAGAGCAAGGTTAGCTAACATCGATGGCGTCAACCAGCACCTTGTAGTGGCACACGGTAATCATTTTCTTCAAATCATTCGGCATCTATCACAAGGACTAAACCTTTCGTTGGATGGAGAGGCACCCATACAAACTGCTATTACGAAGAAAGTATATCCAGTACCTAACCACCAACAGAGAAAGTTAACGCCGGCAAAGTTTGAAGCTTGGAGAATGTGGCATGAAGACAGCCTCTCAATTCAGAAAATTGCTAATTTCCCTGGTAGAGCTGCTCCTATTAAAGAGCAGACTGTTCAAGAATATCTGATGGAAGCTGCTCAAGAAGGATTTGCAATTGATTGGGTCAGATTATGCGATGAGGTTGGACTGACACAAAAAGTTATCTCAGATATTCAGTGTGCCATTTCAAAGGTTGGCTCTAGAGAAAAGTTAAAGCCTATCAAGGACGAATTACCAGAAGATATAAATTATGGACACATCAAGACTTTCCTGACAATGGAAAAGTGTGGGATCTCGCTGGATGGGCCTTTACCTAACCACCACAATGCAGGAAAAGATGATCAACTCCTCAAGAAGGAAACTGAATTGTTACCGAGTTCTATTCATACATCCCCTATGGAAGAACCTCATGAAGTTAAAACCTCAGGCCGAGATTCAGATGCTTACAGCTTGGATAAAAATAAAGAAACAGCTTCCCTTCCATTCAATAGAGAACAAGGTTTAAAGCTACCTGAAGTACATTTTGAGGATTTACTCTCAAAGAAACGCCAAAAGCTTGGTAGTCCCAAGGAGGAGAGTAAAACTACACTGAAGGCAACCGAGAATTCCATATTGGATTGGCTTAGTACCAAAAATGGAGTTTCTCTCTCTGCGATTCTGGAGCACTTCAATGGATCTGAAGAAGACTGTGTCATCGAACTCCTCACTGGCCTTGAAAGTGACTTTATGATATACAGAAAGAACAATATGTATTTCGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

920

Amino Acids

103.38

Weight (kDa)

6.78

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 18 - 179 5.5e-24 DEAD/DEAH box helicase
Helicase_C PF00271 227 - 326 1.3e-21 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 338 - 399 4.8e-14 RecQ zinc-binding
RQC PF09382 403 - 500 9e-24 RQC domain
HRDC PF00570 554 - 619 2.6e-13 HRDC domain
HTH_40 PF14493 661 - 756 5.1e-17 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 1900
AccB7I CCANNNNNTGG 2 cut(s) 503, 1361
AccI GTMKAC 1 cut(s) 1613
AciI CCGC 1 cut(s) 1523
AclWI GGATC 4 cut(s) 248, 395, 2297, 2680
AcoI YGGCCR 2 cut(s) 53, 114
AcsI RAATTY 2 cut(s) 677, 2602
AcuI CTGAAG 4 cut(s) 773, 2532, 2609, 2697
AcyI GRCGYC 1 cut(s) 1806
AfaI GTAC 6 cut(s) 323, 684, 1579, 1941, 2517, 2626
AfiI CCNNNNNNNGG 7 cut(s) 503, 1361, 1785, 1825, 1888, 2275, 2353
AflIII ACRYGT 1 cut(s) 1221
AjnI CCWGG 2 cut(s) 948, 2038
AjuI GAANNNNNNNTTGG 2 cut(s) 2594, 2626
Alw21I GWGCWC 1 cut(s) 2664
Alw26I GTCTC 1 cut(s) 1433
AlwI GGATC 4 cut(s) 248, 395, 2297, 2680
AlwNI CAGNNNCTG 2 cut(s) 363, 1448
Ama87I CYCGRG 1 cut(s) 1314
AoxI GGCC 7 cut(s) 15, 53, 114, 433, 2303, 2425, 2707
ApeKI GCWGC 4 cut(s) 921, 1094, 2048, 2093
ApoI RAATTY 2 cut(s) 677, 2602
AseI ATTAAT 1 cut(s) 1415
Asp700I GAANNNNTTC 3 cut(s) 531, 1161, 1236
AspS9I GGNCC 3 cut(s) 433, 1508, 2303
AsuHPI GGTGA 2 cut(s) 1598, 1748
AsuII TTCGAA 1 cut(s) 535
AvaI CYCGRG 1 cut(s) 1314
AvaII GGWCC 1 cut(s) 1508
AxyI CCTNAGG 1 cut(s) 2422
BalI TGGCCA 1 cut(s) 116
BanI GGYRCC 1 cut(s) 1900
BanII GRGCYC 1 cut(s) 191
BarI GAAGNNNNNNTAC 2 cut(s) 1287, 1319
BauI CACGAG 1 cut(s) 807
BbsI GAAGAC 2 cut(s) 2010, 2688
Bbv12I GWGCWC 1 cut(s) 2664
BbvI GCAGC 4 cut(s) 908, 1106, 2035, 2080
BciT130I CCWGG 2 cut(s) 950, 2040
BclI TGATCA 4 cut(s) 733, 1513, 1738, 2338
BcoDI GTCTC 1 cut(s) 1433
BfaI CTAG 3 cut(s) 1227, 1529, 2199
BfmI CTRYAG 1 cut(s) 1293
BisI GCNGC 4 cut(s) 922, 1095, 2049, 2094
BlsI GCNGC 4 cut(s) 923, 1096, 2050, 2095
Bme1390I CCNGG 2 cut(s) 950, 2040
Bme18I GGWCC 1 cut(s) 1508
BmeT110I CYCGRG 1 cut(s) 1314
BmgT120I GGNCC 3 cut(s) 433, 1508, 2303
BmiI GGNNCC 2 cut(s) 434, 1902
BmrFI CCNGG 2 cut(s) 950, 2040
BmsI GCATC 3 cut(s) 1324, 1870, 2428
BpiI GAAGAC 2 cut(s) 2010, 2688
BplI GAGNNNNNCTC 2 cut(s) 2628, 2660
BpmI CTGGAG 2 cut(s) 1062, 2678
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 3 cut(s) 8, 2082, 2333
Bsa29I ATCGAT 1 cut(s) 1800
BsaAI YACGTR 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 1806
BsaJI CCNNGG 4 cut(s) 784, 1708, 2038, 2565
Bsc4I CCNNNNNNNGG 7 cut(s) 503, 1361, 1785, 1825, 1888, 2275, 2353
Bse118I RCCGGY 1 cut(s) 1969
Bse1I ACTGG 7 cut(s) 124, 178, 849, 1538, 1545, 1937, 2710
Bse21I CCTNAGG 1 cut(s) 2422
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 950, 2040
BseCI ATCGAT 1 cut(s) 1800
BseDI CCNNGG 4 cut(s) 784, 1708, 2038, 2565
BseGI GGATG 4 cut(s) 853, 1897, 2305, 2387
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 7 cut(s) 503, 1361, 1785, 1825, 1888, 2275, 2353
BseMII CTCAG 3 cut(s) 1635, 2178, 2436
BseNI ACTGG 7 cut(s) 124, 178, 849, 1538, 1545, 1937, 2710
BseRI GAGGAG 3 cut(s) 2336, 2585, 2690
BseXI GCAGC 4 cut(s) 908, 1106, 2035, 2080
BseYI CCCAGC 1 cut(s) 383
BsgI GTGCAG 1 cut(s) 697
BshFI GGCC 7 cut(s) 17, 55, 116, 435, 2305, 2427, 2709
BshNI GGYRCC 1 cut(s) 1900
BshVI ATCGAT 1 cut(s) 1800
BsiHKAI GWGCWC 1 cut(s) 2664
BsiHKCI CYCGRG 1 cut(s) 1314
BsiSI CCGG 1 cut(s) 1970
BslFI GGGAC 4 cut(s) 110, 479, 1232, 2547
BslI CCNNNNNNNGG 7 cut(s) 503, 1361, 1785, 1825, 1888, 2275, 2353
BsmAI GTCTC 1 cut(s) 1433
BsmFI GGGAC 4 cut(s) 110, 479, 1232, 2547
BsmI GAATGC 1 cut(s) 1254
BsnI GGCC 7 cut(s) 17, 55, 116, 435, 2305, 2427, 2709
BsoBI CYCGRG 1 cut(s) 1314
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 2 cut(s) 191, 2664
Bsp1407I TGTACA 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 784
BspACI CCGC 1 cut(s) 1523
BspANI GGCC 7 cut(s) 17, 55, 116, 435, 2305, 2427, 2709
BspCNI CTCAG 3 cut(s) 1636, 2177, 2435
BspDI ATCGAT 1 cut(s) 1800
BspHI TCATGA 2 cut(s) 652, 2407
BspLI GGNNCC 2 cut(s) 434, 1902
BspPI GGATC 4 cut(s) 248, 395, 2297, 2680
BspQI GCTCTTC 1 cut(s) 1063
BspT104I TTCGAA 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 1900
BsrFI RCCGGY 1 cut(s) 1969
BsrGI TGTACA 1 cut(s) 321
BsrI ACTGG 7 cut(s) 124, 178, 849, 1538, 1545, 1937, 2710
BssAI RCCGGY 1 cut(s) 1969
BssECI CCNNGG 4 cut(s) 784, 1708, 2038, 2565
BssNI GRCGYC 1 cut(s) 1806
BssSI CACGAG 1 cut(s) 807
BssT1I CCWWGG 3 cut(s) 784, 1708, 2565
Bst2BI CACGAG 1 cut(s) 807
Bst2UI CCWGG 2 cut(s) 950, 2040
Bst4CI ACNGT 8 cut(s) 283, 1104, 1444, 1465, 1665, 1835, 2071, 2687
Bst6I CTCTTC 4 cut(s) 923, 1063, 1426, 1677
BstACI GRCGYC 1 cut(s) 1806
BstAPI GCANNNNNTGC 1 cut(s) 10
BstAUI TGTACA 1 cut(s) 321
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 5 cut(s) 15, 995, 1252, 1698, 1971
BstDEI CTNAG 6 cut(s) 377, 1496, 1644, 2164, 2422, 2621
BstDSI CCRYGG 1 cut(s) 784
BstENI CCTNNNNNAGG 1 cut(s) 2351
BstF5I GGATG 4 cut(s) 853, 1897, 2305, 2387
BstMAI GTCTC 1 cut(s) 1433
BstMWI GCNNNNNNNGC 7 cut(s) 10, 866, 1357, 1657, 1724, 1790, 2302
BstNI CCWGG 2 cut(s) 950, 2040
BstNSI RCATGY 2 cut(s) 345, 1225
BstSCI CCNGG 2 cut(s) 948, 2038
BstSFI CTRYAG 1 cut(s) 1293
BstV1I GCAGC 4 cut(s) 908, 1106, 2035, 2080
BstV2I GAAGAC 2 cut(s) 2010, 2688
BstX2I RGATCY 3 cut(s) 387, 2289, 2672
BstXI CCANNNNNNTGG 3 cut(s) 956, 1552, 2555
BstYI RGATCY 3 cut(s) 387, 2289, 2672
Bsu15I ATCGAT 1 cut(s) 1800
Bsu36I CCTNAGG 1 cut(s) 2422
BsuRI GGCC 7 cut(s) 17, 55, 116, 435, 2305, 2427, 2709
BsuTUI ATCGAT 1 cut(s) 1800
BtgI CCRYGG 1 cut(s) 784
BtgZI GCGATG 1 cut(s) 2148
BtsCI GGATG 4 cut(s) 853, 1897, 2305, 2387
BtsI GCAGTG 2 cut(s) 415, 1355
Cac8I GCNNGC 5 cut(s) 15, 995, 1252, 1698, 1971
CaiI CAGNNNCTG 2 cut(s) 363, 1448
CciI TCATGA 2 cut(s) 652, 2407
Cfr10I RCCGGY 1 cut(s) 1969
Cfr13I GGNCC 3 cut(s) 433, 1508, 2303
ClaI ATCGAT 1 cut(s) 1800
CseI GACGC 1 cut(s) 1795
Csp6I GTAC 6 cut(s) 322, 683, 1578, 1940, 2516, 2625
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviQI GTAC 6 cut(s) 322, 683, 1578, 1940, 2516, 2625
DdeI CTNAG 6 cut(s) 377, 1496, 1644, 2164, 2422, 2621
DraI TTTAAA 1 cut(s) 2502
EaeI YGGCCR 2 cut(s) 53, 114
Eam1104I CTCTTC 4 cut(s) 923, 1063, 1426, 1677
EarI CTCTTC 4 cut(s) 923, 1063, 1426, 1677
Eco130I CCWWGG 3 cut(s) 784, 1708, 2565
Eco24I GRGCYC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 1508
Eco57I CTGAAG 4 cut(s) 773, 2532, 2609, 2697
Eco81I CCTNAGG 1 cut(s) 2422
Eco88I CYCGRG 1 cut(s) 1314
EcoNI CCTNNNNNAGG 1 cut(s) 2351
EcoRI GAATTC 1 cut(s) 2602
EcoRII CCWGG 2 cut(s) 948, 2038
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 784, 1708, 2565
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 784, 1708, 2565
FalI AAGNNNNNCTT 4 cut(s) 85, 117, 1224, 1256
FaqI GGGAC 4 cut(s) 110, 479, 1232, 2547
FauNDI CATATG 2 cut(s) 574, 1723
FbaI TGATCA 4 cut(s) 733, 1513, 1738, 2338
FblI GTMKAC 1 cut(s) 1613
Fnu4HI GCNGC 4 cut(s) 922, 1095, 2049, 2094
FokI GGATG 4 cut(s) 860, 1904, 2312, 2374
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 4 cut(s) 922, 1095, 2049, 2094
FspBI CTAG 3 cut(s) 1227, 1529, 2199
GluI GCNGC 4 cut(s) 922, 1095, 2049, 2094
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 2 cut(s) 1062, 2678
HaeIII GGCC 7 cut(s) 17, 55, 116, 435, 2305, 2427, 2709
HapII CCGG 1 cut(s) 1970
HgaI GACGC 1 cut(s) 1795
Hin1I GRCGYC 1 cut(s) 1806
HincII GTYRAC 2 cut(s) 1810, 1965
HindII GTYRAC 2 cut(s) 1810, 1965
HindIII AAGCTT 4 cut(s) 1349, 1694, 1983, 2552
HpaI GTTAAC 1 cut(s) 1965
HpaII CCGG 1 cut(s) 1970
HphI GGTGA 2 cut(s) 1598, 1748
Hpy166II GTNNAC 4 cut(s) 959, 1614, 1810, 1965
Hpy8I GTNNAC 4 cut(s) 959, 1614, 1810, 1965
HpyCH4III ACNGT 8 cut(s) 283, 1104, 1444, 1465, 1665, 1835, 2071, 2687
HpyCH4IV ACGT 2 cut(s) 475, 543
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 866, 1357, 1657, 1724, 1790, 2302
HpyF3I CTNAG 6 cut(s) 377, 1496, 1644, 2164, 2422, 2621
HpySE526I ACGT 2 cut(s) 475, 543
Hsp92I GRCGYC 1 cut(s) 1806
KroI GCCGGC 1 cut(s) 1969
KroNI GCCGGC 1 cut(s) 1971
Ksp22I TGATCA 4 cut(s) 733, 1513, 1738, 2338
KspAI GTTAAC 1 cut(s) 1965
LguI GCTCTTC 1 cut(s) 1063
LmnI GCTCC 4 cut(s) 1486, 1723, 2056, 2659
Lsp1109I GCAGC 4 cut(s) 908, 1106, 2035, 2080
LweI GCATC 3 cut(s) 1324, 1870, 2428
MaeI CTAG 3 cut(s) 1227, 1529, 2199
MaeII ACGT 2 cut(s) 475, 543
MaeIII GTNAC 5 cut(s) 471, 1145, 1540, 2367, 2717
MfeI CAATTG 3 cut(s) 269, 461, 2112
MflI RGATCY 3 cut(s) 387, 2289, 2672
MhlI GDGCHC 2 cut(s) 191, 2664
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 3 cut(s) 92, 631, 1091
MmeI TCCRAC 4 cut(s) 145, 1573, 1869, 2122
Mox20I TGGCCA 1 cut(s) 116
MroNI GCCGGC 1 cut(s) 1969
MroXI GAANNNNTTC 3 cut(s) 531, 1161, 1236
MscI TGGCCA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 2322, 2667
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 1 cut(s) 1970
MspR9I CCNGG 2 cut(s) 950, 2040
MunI CAATTG 3 cut(s) 269, 461, 2112
Mva1269I GAATGC 1 cut(s) 1254
MvaI CCWGG 2 cut(s) 950, 2040
MwoI GCNNNNNNNGC 7 cut(s) 10, 866, 1357, 1657, 1724, 1790, 2302
NaeI GCCGGC 1 cut(s) 1971
NcoI CCATGG 1 cut(s) 784
NdeI CATATG 2 cut(s) 574, 1723
NgoMIV GCCGGC 1 cut(s) 1969
NlaIV GGNNCC 2 cut(s) 434, 1902
NmeAIII GCCGAG 4 cut(s) 274, 317, 322, 2453
NmuCI GTSAC 3 cut(s) 1145, 1540, 2717
NspI RCATGY 2 cut(s) 345, 1225
NspV TTCGAA 1 cut(s) 535
PagI TCATGA 2 cut(s) 652, 2407
PciI ACATGT 1 cut(s) 1221
PciSI GCTCTTC 1 cut(s) 1063
PctI GAATGC 1 cut(s) 1254
PdiI GCCGGC 1 cut(s) 1971
PdmI GAANNNNTTC 3 cut(s) 531, 1161, 1236
PfeI GAWTC 7 cut(s) 349, 777, 815, 938, 1327, 2432, 2653
PflFI GACNNNGTC 1 cut(s) 2687
PflMI CCANNNNNTGG 2 cut(s) 503, 1361
PkrI GCNGC 4 cut(s) 923, 1096, 2050, 2095
PleI GAGTC 3 cut(s) 92, 631, 1090
PpsI GAGTC 3 cut(s) 92, 631, 1090
Ppu21I YACGTR 1 cut(s) 476
PscI ACATGT 1 cut(s) 1221
PshBI ATTAAT 1 cut(s) 1415
PsiI TTATAA 1 cut(s) 833
Psp6I CCWGG 2 cut(s) 948, 2038
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 2 cut(s) 948, 2038
PspN4I GGNNCC 2 cut(s) 434, 1902
PspPI GGNCC 3 cut(s) 433, 1508, 2303
PstNI CAGNNNCTG 2 cut(s) 363, 1448
PsuI RGATCY 3 cut(s) 387, 2289, 2672
PsyI GACNNNGTC 1 cut(s) 2687
RsaI GTAC 6 cut(s) 323, 684, 1579, 1941, 2517, 2626
RsaNI GTAC 6 cut(s) 322, 683, 1578, 1940, 2516, 2625
RseI CAYNNNNRTG 2 cut(s) 2322, 2667
SapI GCTCTTC 1 cut(s) 1063
SatI GCNGC 4 cut(s) 922, 1095, 2049, 2094
Sau96I GGNCC 3 cut(s) 433, 1508, 2303
SchI GAGTC 3 cut(s) 92, 631, 1091
ScrFI CCNGG 2 cut(s) 950, 2040
SduI GDGCHC 2 cut(s) 191, 2664
SfaNI GCATC 3 cut(s) 1324, 1870, 2428
SfcI CTRYAG 1 cut(s) 1293
SfuI TTCGAA 1 cut(s) 535
SinI GGWCC 1 cut(s) 1508
SmiMI CAYNNNNRTG 2 cut(s) 2322, 2667
SmlI CTYRAG 3 cut(s) 23, 2097, 2348
SmoI CTYRAG 3 cut(s) 23, 2097, 2348
SsiI CCGC 1 cut(s) 1523
SspI AATATT 1 cut(s) 32
SspMI CTAG 3 cut(s) 1227, 1529, 2199
StyD4I CCNGG 2 cut(s) 948, 2038
StyI CCWWGG 3 cut(s) 784, 1708, 2565
TaaI ACNGT 8 cut(s) 283, 1104, 1444, 1465, 1665, 1835, 2071, 2687
TaiI ACGT 2 cut(s) 478, 546
TaqI TCGA 3 cut(s) 535, 1800, 2694
TatI WGTACW 3 cut(s) 321, 1577, 2515
TfiI GAWTC 7 cut(s) 349, 777, 815, 938, 1327, 2432, 2653
TseFI GTSAC 3 cut(s) 1145, 1540, 2717
TseI GCWGC 4 cut(s) 921, 1094, 2048, 2093
Tsp45I GTSAC 3 cut(s) 1145, 1540, 2717
TspDTI ATGAA 7 cut(s) 36, 423, 641, 855, 2016, 2372, 2424
TspGWI ACGGA 1 cut(s) 1451
Tth111I GACNNNGTC 1 cut(s) 2687
Van91I CCANNNNNTGG 2 cut(s) 503, 1361
VpaK11BI GGWCC 1 cut(s) 1508
VspI ATTAAT 1 cut(s) 1415
XagI CCTNNNNNAGG 1 cut(s) 2351
XapI RAATTY 2 cut(s) 677, 2602
XbaI TCTAGA 2 cut(s) 1226, 2198
XceI RCATGY 2 cut(s) 345, 1225
XmiI GTMKAC 1 cut(s) 1613
XmnI GAANNNNTTC 3 cut(s) 531, 1161, 1236
XspI CTAG 3 cut(s) 1227, 1529, 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.