Rh5AG485900

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003692
Physical Location & Seq
Forward (+)
82985384 .. 82991684
6301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG485900.1

Sequence Viewer

Length: 1641 bp
ATGTCGTCTATGCAGGCCATTCTCAAGAAATATGGGTTTTCTTCATTTCGGCCATACCAAAAGAAAGTGATTGAGAAAATCATAGCAGGAAGGGACTCTTTGATTGTCATGGCCACTGGAAGTGGCAAGTCTTTGTGCTATCAAGTGCCTCCTTTGGTTGTTGGAAAGACTGGTGTGGTTGTGAGCCCTCTTTTATCCTTGATGCAAGATCAGGTAATGGCTTTGAAACAAAGAGGGATCAGAGCTGAGTATATGGGTACTAGTCAAACTGATAGCACTGTCCAGAGCCGAGCCGAGAGTGGTCAATTTGATATCTTGTACATGACCCCTGAAAAGGCATGTAAGATTCCTGACAGCTTCTGGTCAAAATTACTAAGGGCTGGAATCTGCCTGTTTGCTGTTGATGAAGCACACTGCATATCAGAGTGGGGCCATGATTTCAGGATGGACTACAAGCGATTGGACAAGTTACGTGGCATTCTTGTTGGTGTCCCATTTATTGGCTTAACTGCAACTGCTACTAAAAAGGTTCAAAGGGACATTGTTAATTCCTTGAAGATGAAAAACCCATATTACAGAATAGGCTCCTTTGATCGTGGAAATGTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTCATGAGGTAGCTCAAGAAGTTTCCAAATTTGTACGCACAGATGGTTCAACTATAATTTACTGCACGACAATTAAAGACGTTTATCAGGTATTCATTGCACTGAAGGAGGTGGGCATTAAGGCTGGAATCTACCATAGTGAGATGGATAATAAAGCTCGTGCCGAATCCCATAGATTATTTATAAGAGATGAAGTAGATGTCATGGTTGCTACTATTGCTTTCGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCATTATGGCTGCCCGAAGAGTTTGGAGTCTTATTACCAGGAAAGTGGTCGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACCTCACAAAAGCTGAATTTTATGCTGGAGAGTGTCAAACAGAAAGTCGACGAAGAGCTGTTATAAAGTCGATGAGGGCAGTACAACAATATTGCTTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTTACTTTGGGGAAATCTTTCCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCCCCAAAGAAGGAGATGGACATGTCTAGAGAAGCATATCTTCTGATGGCTTGCATTCAATCATGTCGGGGTGCATGCGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCTCGGGCCAAAAAGATTATTGCTGCCGAATATGACAAGCTTCCACTGCATGGACTTGGGAAAGACTATTCTGCAAATTGGTGGAAAACACTTGCTTACCAATTAATCTCTTATGATGAAATCAAGAACCTTTGTTTTTCATGCCCCAGTGTCAGTCCGAAAGGGGAGCAATTTCTAAGTTCTGCTGGACCTGATCATCAACAGCCTCTAGTGTTGCCGGTGACCAGTGAAATGGTAGATGATGAGGACAATGAAAGTACATCAGGAGAGAAAGAGATGAAAATGTATGGAGTAGAAAGGTTGAATCTTCGGAAGAGTAGGTCTCGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

546

Amino Acids

61.66

Weight (kDa)

9.02

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 15 - 172 1.7e-06 Type III restriction enzyme, res subunit
DEAD PF00270 17 - 178 3.9e-25 DEAD/DEAH box helicase
Helicase_C PF00271 227 - 325 2e-20 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 337 - 398 1.2e-15 RecQ zinc-binding
RQC PF09382 401 - 495 1.1e-17 RQC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 830, 1076
AccB7I CCANNNNNTGG 2 cut(s) 500, 1358
AccI GTMKAC 1 cut(s) 1060
AciI CCGC 1 cut(s) 1275
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 2 cut(s) 50, 111
AcsI RAATTY 2 cut(s) 674, 1028
AcuI CTGAAG 1 cut(s) 770
AfaI GTAC 5 cut(s) 259, 320, 681, 1095, 1567
AfiI CCNNNNNNNGG 4 cut(s) 334, 500, 1207, 1358
AflIII ACRYGT 1 cut(s) 1218
AgsI TTSAA 7 cut(s) 226, 533, 556, 628, 696, 1256, 1612
AhlI ACTAGT 1 cut(s) 260
AjnI CCWGG 1 cut(s) 945
AluBI AGCT 7 cut(s) 245, 357, 659, 803, 1025, 1070, 1348
AluI AGCT 7 cut(s) 245, 357, 659, 803, 1025, 1070, 1348
Alw26I GTCTC 1 cut(s) 1635
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 360
Ama87I CYCGRG 1 cut(s) 1311
AoxI GGCC 5 cut(s) 15, 50, 111, 430, 1314
ApeKI GCWGC 2 cut(s) 918, 1331
ApoI RAATTY 2 cut(s) 674, 1028
ArsI GACNNNNNNTTYG 2 cut(s) 1042, 1074
AseI ATTAAT 1 cut(s) 1412
Asp700I GAANNNNTTC 1 cut(s) 1158
AspS9I GGNCC 3 cut(s) 430, 1314, 1496
AsuHPI GGTGA 1 cut(s) 1540
AvaI CYCGRG 1 cut(s) 1311
AvaII GGWCC 1 cut(s) 1496
BalI TGGCCA 1 cut(s) 113
BanII GRGCYC 1 cut(s) 188
BarI GAAGNNNNNNTAC 2 cut(s) 1284, 1316
BauI CACGAG 1 cut(s) 804
BbvI GCAGC 2 cut(s) 905, 1318
BccI CCATC 7 cut(s) 439, 683, 784, 965, 1171, 1207, 1237
BciT130I CCWGG 1 cut(s) 947
BclI TGATCA 1 cut(s) 1501
BcoDI GTCTC 1 cut(s) 1635
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 3 cut(s) 261, 1224, 1517
BfmI CTRYAG 1 cut(s) 1290
BisI GCNGC 2 cut(s) 919, 1332
BlsI GCNGC 2 cut(s) 920, 1333
Bme1390I CCNGG 1 cut(s) 947
Bme18I GGWCC 1 cut(s) 1496
BmeT110I CYCGRG 1 cut(s) 1311
BmgT120I GGNCC 3 cut(s) 430, 1314, 1496
BmiI GGNNCC 2 cut(s) 431, 586
BmrFI CCNGG 1 cut(s) 947
BmrI ACTGGG 1 cut(s) 1449
BmsI GCATC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 1449
BplI GAGNNNNNCTC 1 cut(s) 1615
BpmI CTGGAG 1 cut(s) 1059
BpuEI CTTGAG 2 cut(s) 8, 645
BsaAI YACGTR 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 1635
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 500, 1207, 1358
Bse118I RCCGGY 1 cut(s) 1525
Bse1I ACTGG 4 cut(s) 121, 175, 1455, 1533
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 1 cut(s) 947
BseGI GGATG 1 cut(s) 450
BseLI CCNNNNNNNGG 4 cut(s) 334, 500, 1207, 1358
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 4 cut(s) 121, 175, 1455, 1533
BseXI GCAGC 2 cut(s) 905, 1318
BsgI GTGCAG 1 cut(s) 694
BshFI GGCC 5 cut(s) 17, 52, 113, 432, 1316
BsiHKCI CYCGRG 1 cut(s) 1311
BsiSI CCGG 1 cut(s) 1526
BslFI GGGAC 3 cut(s) 107, 476, 551
BslI CCNNNNNNNGG 4 cut(s) 334, 500, 1207, 1358
BsmAI GTCTC 1 cut(s) 1635
BsmFI GGGAC 3 cut(s) 107, 476, 551
BsmI GAATGC 2 cut(s) 477, 1251
BsnI GGCC 5 cut(s) 17, 52, 113, 432, 1316
Bso31I GGTCTC 1 cut(s) 1635
BsoBI CYCGRG 1 cut(s) 1311
Bsp1286I GDGCHC 1 cut(s) 188
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 4 cut(s) 208, 237, 592, 1501
BspACI CCGC 1 cut(s) 1275
BspANI GGCC 5 cut(s) 17, 52, 113, 432, 1316
BspCNI CTCAG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 431, 586
BspPI GGATC 1 cut(s) 245
BspQI GCTCTTC 1 cut(s) 1060
BspTNI GGTCTC 1 cut(s) 1635
BsrDI GCAATG 1 cut(s) 741
BsrFI RCCGGY 1 cut(s) 1525
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 4 cut(s) 121, 175, 1455, 1533
BssAI RCCGGY 1 cut(s) 1525
BssMI GATC 4 cut(s) 208, 237, 592, 1501
BssSI CACGAG 1 cut(s) 804
Bst2BI CACGAG 1 cut(s) 804
Bst2UI CCWGG 1 cut(s) 947
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 3 cut(s) 920, 1060, 1616
BstAUI TGTACA 1 cut(s) 318
BstBAI YACGTR 1 cut(s) 473
BstC8I GCNNGC 4 cut(s) 15, 992, 1249, 1273
BstDEI CTNAG 3 cut(s) 246, 374, 1484
BstEII GGTNACC 1 cut(s) 1528
BstF5I GGATG 1 cut(s) 450
BstKTI GATC 4 cut(s) 211, 240, 595, 1504
BstMAI GTCTC 1 cut(s) 1635
BstMBI GATC 4 cut(s) 208, 237, 592, 1501
BstMWI GCNNNNNNNGC 2 cut(s) 863, 1354
BstNI CCWGG 1 cut(s) 947
BstNSI RCATGY 3 cut(s) 342, 1222, 1275
BstPI GGTNACC 1 cut(s) 1528
BstSCI CCNGG 1 cut(s) 945
BstSFI CTRYAG 1 cut(s) 1290
BstV1I GCAGC 2 cut(s) 905, 1318
BstXI CCANNNNNNTGG 2 cut(s) 953, 1540
BsuRI GGCC 5 cut(s) 17, 52, 113, 432, 1316
BtsCI GGATG 1 cut(s) 450
BtsI GCAGTG 2 cut(s) 412, 1352
BtsIMutI CAGTG 7 cut(s) 114, 276, 412, 746, 1352, 1462, 1540
Cac8I GCNNGC 4 cut(s) 15, 992, 1249, 1273
CaiI CAGNNNCTG 1 cut(s) 360
CciI TCATGA 1 cut(s) 649
Cfr10I RCCGGY 1 cut(s) 1525
Cfr13I GGNCC 3 cut(s) 430, 1314, 1496
Csp6I GTAC 5 cut(s) 258, 319, 680, 1094, 1566
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviQI GTAC 5 cut(s) 258, 319, 680, 1094, 1566
DdeI CTNAG 3 cut(s) 246, 374, 1484
DpnI GATC 4 cut(s) 210, 239, 594, 1503
DpnII GATC 4 cut(s) 208, 237, 592, 1501
EaeI YGGCCR 2 cut(s) 50, 111
Eam1104I CTCTTC 3 cut(s) 920, 1060, 1616
EarI CTCTTC 3 cut(s) 920, 1060, 1616
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 1635
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 1496
Eco57I CTGAAG 1 cut(s) 770
Eco88I CYCGRG 1 cut(s) 1311
Eco91I GGTNACC 1 cut(s) 1528
EcoO65I GGTNACC 1 cut(s) 1528
EcoRII CCWGG 1 cut(s) 945
EcoRV GATATC 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 188
FalI AAGNNNNNCTT 4 cut(s) 82, 114, 1221, 1253
FaqI GGGAC 3 cut(s) 107, 476, 551
FbaI TGATCA 1 cut(s) 1501
FblI GTMKAC 1 cut(s) 1060
Fnu4HI GCNGC 2 cut(s) 919, 1332
FokI GGATG 1 cut(s) 457
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 2 cut(s) 919, 1332
FspBI CTAG 3 cut(s) 261, 1224, 1517
GluI GCNGC 2 cut(s) 919, 1332
GsuI CTGGAG 1 cut(s) 1059
HaeIII GGCC 5 cut(s) 17, 52, 113, 432, 1316
HapII CCGG 1 cut(s) 1526
HincII GTYRAC 1 cut(s) 1061
HindII GTYRAC 1 cut(s) 1061
HindIII AAGCTT 1 cut(s) 1346
HinfI GANTC 8 cut(s) 95, 346, 384, 634, 774, 812, 935, 1612
HpaII CCGG 1 cut(s) 1526
HphI GGTGA 1 cut(s) 1540
Hpy166II GTNNAC 1 cut(s) 1061
Hpy188I TCNGA 8 cut(s) 242, 424, 633, 1168, 1242, 1281, 1467, 1620
Hpy188III TCNNGA 9 cut(s) 25, 283, 350, 442, 650, 662, 1224, 1432, 1572
Hpy8I GTNNAC 1 cut(s) 1061
Hpy99I CGWCG 1 cut(s) 1065
HpyAV CCTTC 4 cut(s) 84, 745, 1119, 1201
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 2 cut(s) 472, 726
HpyF10VI GCNNNNNNNGC 2 cut(s) 863, 1354
HpyF3I CTNAG 3 cut(s) 246, 374, 1484
HpySE526I ACGT 2 cut(s) 472, 726
Ksp22I TGATCA 1 cut(s) 1501
Kzo9I GATC 4 cut(s) 208, 237, 592, 1501
LguI GCTCTTC 1 cut(s) 1060
LmnI GCTCC 2 cut(s) 590, 1474
Lsp1109I GCAGC 2 cut(s) 905, 1318
LweI GCATC 1 cut(s) 192
MaeI CTAG 3 cut(s) 261, 1224, 1517
MaeII ACGT 2 cut(s) 472, 726
MaeIII GTNAC 3 cut(s) 468, 1142, 1528
MalI GATC 4 cut(s) 210, 239, 594, 1503
MboI GATC 4 cut(s) 208, 237, 592, 1501
MboII GAAGA 8 cut(s) 33, 568, 937, 1077, 1229, 1292, 1607, 1633
MhlI GDGCHC 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 113
MluNI TGGCCA 1 cut(s) 113
MlyI GAGTC 3 cut(s) 89, 628, 944
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 9 cut(s) 159, 198, 227, 646, 748, 1025, 1080, 1524, 1546
Mox20I TGGCCA 1 cut(s) 113
MroXI GAANNNNTTC 1 cut(s) 1158
MscI TGGCCA 1 cut(s) 113
MseI TTAA 5 cut(s) 506, 546, 720, 765, 1412
Msp20I TGGCCA 1 cut(s) 113
MspI CCGG 1 cut(s) 1526
MspR9I CCNGG 1 cut(s) 947
Mva1269I GAATGC 2 cut(s) 477, 1251
MvaI CCWGG 1 cut(s) 947
MwoI GCNNNNNNNGC 2 cut(s) 863, 1354
NdeII GATC 4 cut(s) 208, 237, 592, 1501
NlaIV GGNNCC 2 cut(s) 431, 586
NmeAIII GCCGAG 2 cut(s) 314, 319
NmuCI GTSAC 1 cut(s) 1528
NspI RCATGY 3 cut(s) 342, 1222, 1275
PaeI GCATGC 1 cut(s) 1275
PagI TCATGA 1 cut(s) 649
PciI ACATGT 1 cut(s) 1218
PciSI GCTCTTC 1 cut(s) 1060
PctI GAATGC 2 cut(s) 477, 1251
PdmI GAANNNNTTC 1 cut(s) 1158
PfeI GAWTC 5 cut(s) 346, 384, 774, 812, 1612
PflMI CCANNNNNTGG 2 cut(s) 500, 1358
PkrI GCNGC 2 cut(s) 920, 1333
PleI GAGTC 3 cut(s) 89, 628, 943
PpsI GAGTC 3 cut(s) 89, 628, 943
Ppu21I YACGTR 1 cut(s) 473
PscI ACATGT 1 cut(s) 1218
PshBI ATTAAT 1 cut(s) 1412
PsiI TTATAA 2 cut(s) 830, 1076
Psp6I CCWGG 1 cut(s) 945
PspEI GGTNACC 1 cut(s) 1528
PspGI CCWGG 1 cut(s) 945
PspN4I GGNNCC 2 cut(s) 431, 586
PspPI GGNCC 3 cut(s) 430, 1314, 1496
PstNI CAGNNNCTG 1 cut(s) 360
RsaI GTAC 5 cut(s) 259, 320, 681, 1095, 1567
RsaNI GTAC 5 cut(s) 258, 319, 680, 1094, 1566
SalI GTCGAC 1 cut(s) 1059
SapI GCTCTTC 1 cut(s) 1060
SaqAI TTAA 5 cut(s) 506, 546, 720, 765, 1412
SatI GCNGC 2 cut(s) 919, 1332
Sau3AI GATC 4 cut(s) 208, 237, 592, 1501
Sau96I GGNCC 3 cut(s) 430, 1314, 1496
SchI GAGTC 3 cut(s) 89, 628, 944
ScrFI CCNGG 1 cut(s) 947
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 192
SfcI CTRYAG 1 cut(s) 1290
SinI GGWCC 1 cut(s) 1496
SmlI CTYRAG 2 cut(s) 23, 660
SmoI CTYRAG 2 cut(s) 23, 660
SpeI ACTAGT 1 cut(s) 260
SphI GCATGC 1 cut(s) 1275
SsiI CCGC 1 cut(s) 1275
SspI AATATT 1 cut(s) 1103
SspMI CTAG 3 cut(s) 261, 1224, 1517
StyD4I CCNGG 1 cut(s) 945
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 2 cut(s) 475, 729
TaqI TCGA 3 cut(s) 958, 1060, 1082
TatI WGTACW 3 cut(s) 318, 1093, 1565
TfiI GAWTC 5 cut(s) 346, 384, 774, 812, 1612
Tru1I TTAA 5 cut(s) 506, 546, 720, 765, 1412
Tru9I TTAA 5 cut(s) 506, 546, 720, 765, 1412
TscAI CASTG 7 cut(s) 121, 283, 419, 753, 1359, 1462, 1540
TseFI GTSAC 1 cut(s) 1528
TseI GCWGC 2 cut(s) 918, 1331
Tsp45I GTSAC 1 cut(s) 1528
TspRI CASTG 7 cut(s) 121, 283, 419, 753, 1359, 1462, 1540
Van91I CCANNNNNTGG 2 cut(s) 500, 1358
VpaK11BI GGWCC 1 cut(s) 1496
VspI ATTAAT 1 cut(s) 1412
XapI RAATTY 2 cut(s) 674, 1028
XbaI TCTAGA 1 cut(s) 1223
XceI RCATGY 3 cut(s) 342, 1222, 1275
XmiI GTMKAC 1 cut(s) 1060
XmnI GAANNNNTTC 1 cut(s) 1158
XspI CTAG 3 cut(s) 261, 1224, 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.