Rh5DG518800

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003692
Physical Location & Seq
Forward (+)
80611751 .. 80619944
8194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG518800.1

Sequence Viewer

Length: 2547 bp
ATGGTCTCGGTAATGTCTTTGAAACAAAGAGGGATCAGAGCCGAGTATATGGGTAGTAGTCAATTGGATAACACTGTCCAGAGCCGAGCCGAGAGTGGTCAATTTGATATCTTGTACATGACACCTGAAAAGGCATGTGTGATTCCTGTCAGCTTCTGGTCAAAATTACTAAGGTCTGGGATCTGCTTGTTTGCTGTTGATGAAGCACACTGCATATCGGAGTGGGGCCATGATTTCAGGGTGGAATACAAGCAATTGGACAAGTTACGTGGCGTTCTTGTTGGTGTCCCATTTATTGGCTTAACTGCAACTGCTACTGAAAAGGTTCGAATGGACATTGTTAATTCCTTGAAGATGGAAAACCCATATGTCAAAATAGGTTCATTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTCATGAGCTGGTTCAAGAAGTTTCCAAATTTGTACGCACAGATGGTTCAACAATAATATACTGCACGACAATTAAAGATGTTGATCAGGTATTCAATTCACTGAAGGAGGTGGGCATTAAAGCTGGAATCTACCATGGTCAAATGGATAATAAAGCTCGTGCCGAATCCCATAGATTGTTTATAAGAGATGAACTGGATGTCATGGTTGCCACAATTGCTTTTGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCACTATGGCTGCCCGAAGAGTTTGGAGTCTTATTACCAGGAAAGTGGACGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACTTCGCAAAAGCTGACTTTTATGCTGGAGAGTGTCAAACAGAAAGTCAACGAAGAGCTGTTGTAGAGTCATTGATGGCAGCACAACAGTATTGCTTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTCACTTTGGGGAAATCTTTTCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCTTCAAAGAAGGAGAGGGACATGTCTAGAGAAGCATTTCTTCTAATGGCTTGCATTCAATCATGTCGGGGTAAATGGGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCTCGGGCGAAAAAGATTCTTGATGCCCAATATGACAAGCTTCCACTGCATGGACTTGGGAAAGAGTATTCAGCAAATTGGTGGAAAGCACTTGCTTACCAATTAATCTCTTCTGGTTATTTGACGGAGACGGTATCTGATATATACAGGACTGTAAGTGTCAGCCGGAAAGGGGAGCAATTTCTAAGTTCTGCTGGACCTGATCATCAACCGCCTCTAGTTTTGCCAGTGACCAGTGAAATGGTAGATGATGAGGACAATAAAAGTACATCAGGTGAAGTTGGAGAAATTAAAAGTTTGTCTACTCTGGAATGTGAAGGATTTTCGGAGGCTGAGAAGCAACTGTATCACTCACTTCTTGAAGAGAGAAGGAAGCTTGCAAGAAGCCTTGGAACTGCTCCATATGCTATTTGTGGTGATCAAGCAATCAAAAAAATTGCTTTAACAAGACCATCTACCAGAGCAAGGTTAGCTAACATCGATGGCGTCAACCAGCACCTTGTAGTGGCACACGGTAATCATTTTCTTCAAATCATTCGGCATCTATCACAAGGACTAAACCTTTCGTTGGATGGAGAGGCACCCATACAAGCTGCTATTACGAAGAAAGTATATCCAGTACCTAACCACCAACAGAGAAAGTTAACACCGGCAAAGTTTGAAGCTTGGAGAATGTGGCATGAAGACGGCCTCTCAGTTCAGAAAATTGCTAATTTCCCTGGTAGAGCTGCTCCTATTAAAGAGCAGACTGTTCAAGAATATCTGATGGAAGCTGCTCAAGAAGGATTTGCAATTGATTGGGTCAGATTATGCGATGAGGTTGGACTGACACAAAAAGTTATCTCAGATATTCAGTGTGCCATTTCAAAGGTTGGCTCTAGAGAAAAGTTAAAGCCTATCAAGGACGAATTACCAGAAGATATAAGTTATGGACACATCAAGACTTTCCTGACAATGGAAAAGTGTGGGATCTCGCTGGATGGGCCTTTACCTAACCACCACAATGCAGGAAAAGATGATCAACTCCTCAAGAAGGAAACTGAATTGTCACCGAGTTCTATTCATACATCCCCTATGGAAGAACCTCATGAAGTTAAAACCTCAGGCCGAGATTCAGATTCTTACAGCTTGGATAAAAATAAAGAAACAGCTTCCCTTCCATTCAATAGAGAACAAGGTTTAAAGCTACCTGAAGTACATTTTGAGGATTTACTCTCAAAGAAACGCCAAAAGCTTGGTAGTCCCAAGGAGGAGAGTAAAACTACACTGAAGGCAACCGAGAATTCCATATTGGATTGGCTTAGTACCAAAAATGGAGTTACTCTCTCTGCTATTCTGGAGCACTTCAATGGATCTGAAGATGACTGTGTCATTGAACTCCTCACTGGCCTTGAAAGTGACTTTATGATATACAGAAAGAACAATATGTATTTCGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

848

Amino Acids

95.34

Weight (kDa)

6.66

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 23 - 110 1.3e-06 DEAD/DEAH box helicase
Helicase_C PF00271 158 - 257 1.2e-21 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 269 - 330 8.8e-14 RecQ zinc-binding
RQC PF09382 334 - 431 1.7e-24 RQC domain
HRDC PF00570 481 - 547 1e-13 HRDC domain
HTH_40 PF14493 589 - 684 2.5e-18 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 626
AccB1I GGYRCC 1 cut(s) 1684
AccB7I CCANNNNNTGG 2 cut(s) 296, 1154
AccI GTMKAC 1 cut(s) 1406
AciI CCGC 1 cut(s) 1316
AclWI GGATC 4 cut(s) 41, 188, 2081, 2464
AcsI RAATTY 2 cut(s) 470, 2386
AcuI CTGAAG 4 cut(s) 566, 2316, 2393, 2481
AcyI GRCGYC 1 cut(s) 1590
AfaI GTAC 6 cut(s) 116, 477, 1372, 1725, 2301, 2410
AfiI CCNNNNNNNGG 8 cut(s) 296, 1154, 1276, 1569, 1609, 1672, 2059, 2137
AflIII ACRYGT 1 cut(s) 1014
AjnI CCWGG 2 cut(s) 741, 1822
AjuI GAANNNNNNNTTGG 2 cut(s) 2378, 2410
Alw21I GWGCWC 1 cut(s) 2448
Alw26I GTCTC 2 cut(s) 10, 1226
AlwI GGATC 4 cut(s) 41, 188, 2081, 2464
AlwNI CAGNNNCTG 1 cut(s) 156
Ama87I CYCGRG 1 cut(s) 1107
AoxI GGCC 5 cut(s) 226, 1792, 2087, 2209, 2491
ApeKI GCWGC 5 cut(s) 714, 887, 1697, 1832, 1877
ApoI RAATTY 2 cut(s) 470, 2386
ArsI GACNNNNNNTTYG 2 cut(s) 838, 870
AseI ATTAAT 1 cut(s) 1208
Asp700I GAANNNNTTC 2 cut(s) 324, 1029
AspS9I GGNCC 3 cut(s) 226, 1301, 2087
AsuHPI GGTGA 3 cut(s) 1391, 1532, 2145
AsuII TTCGAA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 1107
AvaII GGWCC 1 cut(s) 1301
AxyI CCTNAGG 1 cut(s) 2206
BanI GGYRCC 1 cut(s) 1684
BarI GAAGNNNNNNTAC 2 cut(s) 1080, 1112
BauI CACGAG 1 cut(s) 600
BbsI GAAGAC 1 cut(s) 1794
Bbv12I GWGCWC 1 cut(s) 2448
BbvI GCAGC 5 cut(s) 701, 899, 1684, 1819, 1864
BccI CCATC 9 cut(s) 349, 479, 761, 877, 1564, 1580, 1670, 1864, 2078
BceAI ACGGC 1 cut(s) 1807
BciT130I CCWGG 2 cut(s) 743, 1824
BclI TGATCA 4 cut(s) 526, 1306, 1522, 2122
BcoDI GTCTC 2 cut(s) 10, 1226
BfaI CTAG 3 cut(s) 1020, 1322, 1983
BfmI CTRYAG 1 cut(s) 1086
BisI GCNGC 5 cut(s) 715, 888, 1698, 1833, 1878
BlsI GCNGC 5 cut(s) 716, 889, 1699, 1834, 1879
Bme1390I CCNGG 2 cut(s) 743, 1824
Bme18I GGWCC 1 cut(s) 1301
BmeT110I CYCGRG 1 cut(s) 1107
BmgT120I GGNCC 3 cut(s) 226, 1301, 2087
BmiI GGNNCC 2 cut(s) 227, 1686
BmrFI CCNGG 2 cut(s) 743, 1824
BmsI GCATC 2 cut(s) 1117, 1654
BpiI GAAGAC 1 cut(s) 1794
BplI GAGNNNNNCTC 2 cut(s) 2412, 2444
BpmI CTGGAG 2 cut(s) 855, 2462
Bpu14I TTCGAA 1 cut(s) 328
BpuEI CTTGAG 2 cut(s) 1866, 2117
Bsa29I ATCGAT 1 cut(s) 1584
BsaAI YACGTR 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 1590
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 4 cut(s) 577, 1492, 1822, 2349
Bsc4I CCNNNNNNNGG 8 cut(s) 296, 1154, 1276, 1569, 1609, 1672, 2059, 2137
Bse118I RCCGGY 1 cut(s) 1753
Bse1I ACTGG 5 cut(s) 642, 1331, 1338, 1721, 2494
Bse21I CCTNAGG 1 cut(s) 2206
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 2 cut(s) 743, 1824
BseCI ATCGAT 1 cut(s) 1584
BseDI CCNNGG 4 cut(s) 577, 1492, 1822, 2349
BseGI GGATG 4 cut(s) 646, 1681, 2089, 2171
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 8 cut(s) 296, 1154, 1276, 1569, 1609, 1672, 2059, 2137
BseMII CTCAG 4 cut(s) 1428, 1812, 1962, 2220
BseNI ACTGG 5 cut(s) 642, 1331, 1338, 1721, 2494
BseRI GAGGAG 3 cut(s) 2120, 2369, 2474
BseXI GCAGC 5 cut(s) 701, 899, 1684, 1819, 1864
BsgI GTGCAG 1 cut(s) 490
BshFI GGCC 5 cut(s) 228, 1794, 2089, 2211, 2493
BshNI GGYRCC 1 cut(s) 1684
BshVI ATCGAT 1 cut(s) 1584
BsiHKAI GWGCWC 1 cut(s) 2448
BsiHKCI CYCGRG 1 cut(s) 1107
BsiSI CCGG 2 cut(s) 1270, 1754
BslFI GGGAC 3 cut(s) 272, 1025, 2331
BslI CCNNNNNNNGG 8 cut(s) 296, 1154, 1276, 1569, 1609, 1672, 2059, 2137
BsmAI GTCTC 2 cut(s) 10, 1226
BsmBI CGTCTC 1 cut(s) 1226
BsmFI GGGAC 3 cut(s) 272, 1025, 2331
BsmI GAATGC 1 cut(s) 1047
BsnI GGCC 5 cut(s) 228, 1794, 2089, 2211, 2493
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 1 cut(s) 1107
Bsp119I TTCGAA 1 cut(s) 328
Bsp1286I GDGCHC 1 cut(s) 2448
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 1 cut(s) 1316
BspANI GGCC 5 cut(s) 228, 1794, 2089, 2211, 2493
BspCNI CTCAG 4 cut(s) 1429, 1811, 1961, 2219
BspDI ATCGAT 1 cut(s) 1584
BspHI TCATGA 2 cut(s) 445, 2191
BspLI GGNNCC 2 cut(s) 227, 1686
BspPI GGATC 4 cut(s) 41, 188, 2081, 2464
BspQI GCTCTTC 1 cut(s) 856
BspT104I TTCGAA 1 cut(s) 328
BspT107I GGYRCC 1 cut(s) 1684
BspTNI GGTCTC 1 cut(s) 10
BsrFI RCCGGY 1 cut(s) 1753
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 5 cut(s) 642, 1331, 1338, 1721, 2494
BssAI RCCGGY 1 cut(s) 1753
BssECI CCNNGG 4 cut(s) 577, 1492, 1822, 2349
BssMI GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
BssNI GRCGYC 1 cut(s) 1590
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 3 cut(s) 577, 1492, 2349
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 743, 1824
Bst4CI ACNGT 8 cut(s) 76, 897, 1237, 1258, 1449, 1619, 1855, 2471
Bst6I CTCTTC 4 cut(s) 716, 856, 1219, 1461
BstACI GRCGYC 1 cut(s) 1590
BstAUI TGTACA 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 269
BstBI TTCGAA 1 cut(s) 328
BstC8I GCNNGC 3 cut(s) 788, 1045, 1482
BstDEI CTNAG 7 cut(s) 170, 1289, 1437, 1798, 1948, 2206, 2405
BstDSI CCRYGG 1 cut(s) 577
BstENI CCTNNNNNAGG 1 cut(s) 2135
BstF5I GGATG 4 cut(s) 646, 1681, 2089, 2171
BstKTI GATC 9 cut(s) 36, 183, 391, 529, 1309, 1525, 2076, 2125, 2459
BstMAI GTCTC 2 cut(s) 10, 1226
BstMBI GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
BstMWI GCNNNNNNNGC 5 cut(s) 659, 1150, 1508, 1574, 2086
BstNI CCWGG 2 cut(s) 743, 1824
BstNSI RCATGY 2 cut(s) 138, 1018
BstSCI CCNGG 2 cut(s) 741, 1822
BstSFI CTRYAG 1 cut(s) 1086
BstV1I GCAGC 5 cut(s) 701, 899, 1684, 1819, 1864
BstV2I GAAGAC 1 cut(s) 1794
BstX2I RGATCY 3 cut(s) 180, 2073, 2456
BstXI CCANNNNNNTGG 3 cut(s) 749, 1345, 2339
BstYI RGATCY 3 cut(s) 180, 2073, 2456
Bsu15I ATCGAT 1 cut(s) 1584
Bsu36I CCTNAGG 1 cut(s) 2206
BsuRI GGCC 5 cut(s) 228, 1794, 2089, 2211, 2493
BsuTUI ATCGAT 1 cut(s) 1584
BtgI CCRYGG 1 cut(s) 577
BtgZI GCGATG 1 cut(s) 1932
BtsCI GGATG 4 cut(s) 646, 1681, 2089, 2171
BtsI GCAGTG 2 cut(s) 208, 1148
BtsIMutI CAGTG 9 cut(s) 72, 208, 542, 1148, 1338, 1345, 1964, 2369, 2487
Cac8I GCNNGC 3 cut(s) 788, 1045, 1482
CaiI CAGNNNCTG 1 cut(s) 156
CciI TCATGA 2 cut(s) 445, 2191
Cfr10I RCCGGY 1 cut(s) 1753
Cfr13I GGNCC 3 cut(s) 226, 1301, 2087
ClaI ATCGAT 1 cut(s) 1584
CseI GACGC 1 cut(s) 1579
Csp6I GTAC 6 cut(s) 115, 476, 1371, 1724, 2300, 2409
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviQI GTAC 6 cut(s) 115, 476, 1371, 1724, 2300, 2409
DdeI CTNAG 7 cut(s) 170, 1289, 1437, 1798, 1948, 2206, 2405
DpnI GATC 9 cut(s) 35, 182, 390, 528, 1308, 1524, 2075, 2124, 2458
DpnII GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
DraI TTTAAA 1 cut(s) 2286
Eam1104I CTCTTC 4 cut(s) 716, 856, 1219, 1461
EarI CTCTTC 4 cut(s) 716, 856, 1219, 1461
Eco130I CCWWGG 3 cut(s) 577, 1492, 2349
Eco31I GGTCTC 1 cut(s) 10
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 1301
Eco57I CTGAAG 4 cut(s) 566, 2316, 2393, 2481
Eco81I CCTNAGG 1 cut(s) 2206
Eco88I CYCGRG 1 cut(s) 1107
EcoNI CCTNNNNNAGG 1 cut(s) 2135
EcoRI GAATTC 1 cut(s) 2386
EcoRII CCWGG 2 cut(s) 741, 1822
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 3 cut(s) 577, 1492, 2349
ErhI CCWWGG 3 cut(s) 577, 1492, 2349
Esp3I CGTCTC 1 cut(s) 1226
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 3 cut(s) 272, 1025, 2331
FauNDI CATATG 2 cut(s) 367, 1507
FbaI TGATCA 4 cut(s) 526, 1306, 1522, 2122
FblI GTMKAC 1 cut(s) 1406
Fnu4HI GCNGC 5 cut(s) 715, 888, 1698, 1833, 1878
FokI GGATG 4 cut(s) 653, 1688, 2096, 2158
Fsp4HI GCNGC 5 cut(s) 715, 888, 1698, 1833, 1878
FspBI CTAG 3 cut(s) 1020, 1322, 1983
GluI GCNGC 5 cut(s) 715, 888, 1698, 1833, 1878
GsuI CTGGAG 2 cut(s) 855, 2462
HaeIII GGCC 5 cut(s) 228, 1794, 2089, 2211, 2493
HapII CCGG 2 cut(s) 1270, 1754
HgaI GACGC 1 cut(s) 1579
Hin1I GRCGYC 1 cut(s) 1590
HincII GTYRAC 3 cut(s) 857, 1594, 1749
HindII GTYRAC 3 cut(s) 857, 1594, 1749
HindIII AAGCTT 4 cut(s) 1142, 1478, 1767, 2336
HinfI GANTC 9 cut(s) 142, 430, 570, 608, 731, 875, 1120, 2216, 2222
HpaI GTTAAC 1 cut(s) 1749
HpaII CCGG 2 cut(s) 1270, 1754
HphI GGTGA 3 cut(s) 1391, 1532, 2145
Hpy166II GTNNAC 5 cut(s) 752, 857, 1407, 1594, 1749
Hpy8I GTNNAC 5 cut(s) 752, 857, 1407, 1594, 1749
HpyAV CCTTC 9 cut(s) 541, 915, 997, 1415, 1467, 1880, 2131, 2270, 2368
HpyCH4III ACNGT 8 cut(s) 76, 897, 1237, 1258, 1449, 1619, 1855, 2471
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 9 cut(s) 213, 308, 507, 918, 1047, 1153, 1484, 1895, 2111
HpyF10VI GCNNNNNNNGC 5 cut(s) 659, 1150, 1508, 1574, 2086
HpyF3I CTNAG 7 cut(s) 170, 1289, 1437, 1798, 1948, 2206, 2405
HpySE526I ACGT 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 1590
Ksp22I TGATCA 4 cut(s) 526, 1306, 1522, 2122
KspAI GTTAAC 1 cut(s) 1749
Kzo9I GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
LguI GCTCTTC 1 cut(s) 856
LmnI GCTCC 4 cut(s) 1279, 1507, 1840, 2443
Lsp1109I GCAGC 5 cut(s) 701, 899, 1684, 1819, 1864
LweI GCATC 2 cut(s) 1117, 1654
MaeI CTAG 3 cut(s) 1020, 1322, 1983
MaeII ACGT 1 cut(s) 268
MaeIII GTNAC 6 cut(s) 264, 938, 1333, 2151, 2422, 2501
MalI GATC 9 cut(s) 35, 182, 390, 528, 1308, 1524, 2075, 2124, 2458
MboI GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
MfeI CAATTG 4 cut(s) 62, 254, 657, 1896
MflI RGATCY 3 cut(s) 180, 2073, 2456
MhlI GDGCHC 1 cut(s) 2448
MlyI GAGTC 3 cut(s) 424, 740, 884
MmeI TCCRAC 3 cut(s) 1366, 1653, 1906
MroXI GAANNNNTTC 2 cut(s) 324, 1029
MslI CAYNNNNRTG 2 cut(s) 2106, 2451
MspI CCGG 2 cut(s) 1270, 1754
MspR9I CCNGG 2 cut(s) 743, 1824
MunI CAATTG 4 cut(s) 62, 254, 657, 1896
Mva1269I GAATGC 1 cut(s) 1047
MvaI CCWGG 2 cut(s) 743, 1824
MwoI GCNNNNNNNGC 5 cut(s) 659, 1150, 1508, 1574, 2086
NcoI CCATGG 1 cut(s) 577
NdeI CATATG 2 cut(s) 367, 1507
NdeII GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
NlaIV GGNNCC 2 cut(s) 227, 1686
NmeAIII GCCGAG 4 cut(s) 67, 110, 115, 2237
NmuCI GTSAC 4 cut(s) 938, 1333, 2151, 2501
NspI RCATGY 2 cut(s) 138, 1018
NspV TTCGAA 1 cut(s) 328
PagI TCATGA 2 cut(s) 445, 2191
PciI ACATGT 1 cut(s) 1014
PciSI GCTCTTC 1 cut(s) 856
PctI GAATGC 1 cut(s) 1047
PdmI GAANNNNTTC 2 cut(s) 324, 1029
PfeI GAWTC 6 cut(s) 142, 570, 608, 1120, 2216, 2222
PflFI GACNNNGTC 1 cut(s) 2471
PflMI CCANNNNNTGG 2 cut(s) 296, 1154
PkrI GCNGC 5 cut(s) 716, 889, 1699, 1834, 1879
PleI GAGTC 3 cut(s) 424, 739, 883
PpsI GAGTC 3 cut(s) 424, 739, 883
Ppu21I YACGTR 1 cut(s) 269
PscI ACATGT 1 cut(s) 1014
PshBI ATTAAT 1 cut(s) 1208
PsiI TTATAA 1 cut(s) 626
Psp6I CCWGG 2 cut(s) 741, 1822
PspGI CCWGG 2 cut(s) 741, 1822
PspN4I GGNNCC 2 cut(s) 227, 1686
PspPI GGNCC 3 cut(s) 226, 1301, 2087
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 3 cut(s) 180, 2073, 2456
PsyI GACNNNGTC 1 cut(s) 2471
RsaI GTAC 6 cut(s) 116, 477, 1372, 1725, 2301, 2410
RsaNI GTAC 6 cut(s) 115, 476, 1371, 1724, 2300, 2409
RseI CAYNNNNRTG 2 cut(s) 2106, 2451
SapI GCTCTTC 1 cut(s) 856
SatI GCNGC 5 cut(s) 715, 888, 1698, 1833, 1878
Sau3AI GATC 9 cut(s) 33, 180, 388, 526, 1306, 1522, 2073, 2122, 2456
Sau96I GGNCC 3 cut(s) 226, 1301, 2087
SchI GAGTC 3 cut(s) 424, 740, 884
ScrFI CCNGG 2 cut(s) 743, 1824
SduI GDGCHC 1 cut(s) 2448
SfaNI GCATC 2 cut(s) 1117, 1654
SfcI CTRYAG 1 cut(s) 1086
SfuI TTCGAA 1 cut(s) 328
SinI GGWCC 1 cut(s) 1301
SmiMI CAYNNNNRTG 2 cut(s) 2106, 2451
SmlI CTYRAG 2 cut(s) 1881, 2132
SmoI CTYRAG 2 cut(s) 1881, 2132
SsiI CCGC 1 cut(s) 1316
SspMI CTAG 3 cut(s) 1020, 1322, 1983
StyD4I CCNGG 2 cut(s) 741, 1822
StyI CCWWGG 3 cut(s) 577, 1492, 2349
TaaI ACNGT 8 cut(s) 76, 897, 1237, 1258, 1449, 1619, 1855, 2471
TaiI ACGT 1 cut(s) 271
TaqI TCGA 2 cut(s) 328, 1584
TatI WGTACW 3 cut(s) 114, 1370, 2299
TfiI GAWTC 6 cut(s) 142, 570, 608, 1120, 2216, 2222
TscAI CASTG 9 cut(s) 79, 215, 549, 1155, 1338, 1345, 1964, 2376, 2494
TseFI GTSAC 4 cut(s) 938, 1333, 2151, 2501
TseI GCWGC 5 cut(s) 714, 887, 1697, 1832, 1877
Tsp45I GTSAC 4 cut(s) 938, 1333, 2151, 2501
TspDTI ATGAA 8 cut(s) 216, 372, 434, 648, 948, 1800, 2156, 2208
TspGWI ACGGA 1 cut(s) 1244
TspRI CASTG 9 cut(s) 79, 215, 549, 1155, 1338, 1345, 1964, 2376, 2494
Tth111I GACNNNGTC 1 cut(s) 2471
Van91I CCANNNNNTGG 2 cut(s) 296, 1154
VpaK11BI GGWCC 1 cut(s) 1301
VspI ATTAAT 1 cut(s) 1208
XagI CCTNNNNNAGG 1 cut(s) 2135
XapI RAATTY 2 cut(s) 470, 2386
XbaI TCTAGA 2 cut(s) 1019, 1982
XceI RCATGY 2 cut(s) 138, 1018
XmiI GTMKAC 1 cut(s) 1406
XmnI GAANNNNTTC 2 cut(s) 324, 1029
XspI CTAG 3 cut(s) 1020, 1322, 1983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.