MD03G1046200.v1.1

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003692
Physical Location & Seq
Reverse (-)
3717542 .. 3725364
7823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1046200.v1.1.491

Sequence Viewer

Length: 2742 bp
ATGCAGTCCATTCTCAAGAAATATTTTGGGTTTTCTTCGTTTCGACCGTACCAGAAGGAAGTGATTGAGAAAATCATAGACGGAAACGACTCATTGATTGTGATGGCCACCGGCAGCGGCAAGTCGTTGTGCTATCAGGTGCCTCCGTTGGTTGTCGGAAAGACTGGTGTTGTTGTGAGCCCTCTTATATCCCTAATGCAAGATCAGGTAATGTCTTTGAAGCAACGAGGAATTAGAGCCGAGTTTATGGGAAGTAGCCAAACTGATTCCACTGTCCAGAGCAGAGCTGAAAGTGGTCAATTTGATATCTTATACATGACCCCGGAAAAGGCATGTTTGGTTCCTTCAAGTTTCTGGTCCAATTTGCTAAGTGTTGGACTCTGTTTGTTTGCTGTTGATGAAGCACATTGCATATCGGAGTGGGGCCATGATTTCAGGGTAGAATACAAGAAACTAGACAAGTTACGTGGCCTTCTTGTTGATGTTCCATTTATTGCCTTAACTGCAACTGCTACTGAAAAGGTTCGGATGGACATTGTTAATTCCTTGAAGATGCAAAACCCATATGTTGCCATAGGCTCATTTGACCGGACAAATCTGTTCTATGGTGTCAAGTCATTCAATCGTGGTCAGTCATTTGTTCAGGAGCTCGTTCAAGAAGTTTCAACATTTGTGCGCTCAGATGGTTCAACTATCATCTACTGCACGACAATTAAAGATGTTGAGCAGGTATTCGAGTCACTCAAGGAGGTAGGTATTAAGGCTGGAATCTATCATGGTCAAATGGACAATAAAGCTCGTGCGGAGTCCCATAGATTGTTTGTACGAGATGAACTCGATGTCATGGTTGCTACAATTGCTTTTGGAATGGGTATTGACAAACCAAACATAAGGCAAGTGATACATTATGGTTGCCCAAAGAGCTTGGAGTCTTATTACCAGGAAAGTGGACGATGCGGTAGAGATGGTATTGCATCTGTCTGCTGGCTTTATTACACTAGAAGTGACTTTGCAAAAGCTGACTTTTACATTGGAGAGCTACATTCTGAAAGTCAACGTAAAGCTGTTGTAGAGTCGTTGATGGCAGCACAACGGTACTGCTTACTGACAACTTGCAGAAGGAAGTTTTTACTTGGTCACTTTGGGGAAAAGTTTCCAGCTGATAAATGCGGTAATTGTGATAACTGCACTTCCACAAAGAGGGAGCGAGACATGTCTAAAGAAGCATTTCTTCTAATGGCTTGCATTCAATCATGTAGGGGTAAATGGGGTCTCAGTATGCCTGTAGACATTCTTCGTGGCTCTCGAGCTAAAAAAATTATTGATGCCCAATATGACAAGCTTCCACTGCATGGGCTTGGGAAAGACTATTCTTCGAATTGGTGGAAAGCCCTTGGTTACCAATTAGTCTCTTCTGGTTATTTGAGGGAGACAGTAAAAGACATATACAGAACTGTAAGTCTTAGTCCAAAAGCTTACCAATTTCTTAGTTCTGCTGGACCGGATCATCAACCACCCTTATTCTTGCCAGTGACCAGTGAAATGGTCGATGATGAGGACAATAAACATGCATCAGGCGAAGTTGGAGAAATTAAGAGTTTGGCTACTCAGGAATGTGAAGGATTCTCAGAGGCAGAGAAACAACTCTACCACATGCTTTTAGAAGAGAGAAGGAAGCTCGCAAGAAGCCTTGGAACCGCTCCATATGCTATTTGTGGTGATCAAACAATTAAAAAAATTGCTTTAGCAAGACCATCTACCAAAGCAAGGCTAGCAAACATCGATGGCGTCAACCAGCACCTTGTAGTGACTCATGGAAACAATTTTCTTCGGATCATTCGGGATCTATCACAAGGACTAAATCTTTCATTGGATGGAGAGGCAACCGTACAAACTACTGCTATTACGAGAAAAGTCTATCCTGTACCCAACCAACCAAGAAAGTTAACGCCAGCAAAGTTTGAAGCCTGGAAATTGTGGCATGTTGAGGGTTTCTCGATTCAGAAAATTGCTAACTTCCCTGGTAGATCTGCACCTATTAAAGAGCAGACTGTTCTTGATTATGTGGTAGAAGCTGCTCAAGAAGGATGCGAAATTGATTGGATCAGGCTATGCAACGAGGTTGGACTAACGCATAAAGTTCTCTCCGATATTCAGTGTGCCATTTCAAAGGTTGGCCCTACGGAAAGGTTAAAGCCTATCAAAGACGAATTGCCAGAAGATATAAGTTATGCACACATCAAGACTTGCCTGGCAATGCAAAAGCTTGGAGTGTCTCTGGAAGGGACTCCATCTAGCCCCCATGATGCACAAGAAGCTGGTCAACTTCCAAGCAAGGAAACAGAATCGTCACCATGTTCTGCTCGTAAATCCCCCACGGAAGAACCTCTTGAAGATAAAGCCTTGGCCCAAGATTCAGTTGCAAGCTCGGGTAAAAATGAAGAAACCACTCCTCTTCCCTTGACCAGGGGACAAGGAGTAAACCAACCTGAAGCACATTTCGAGGACTTACTCCCAACAAAACGCCAAAAACTTGGTAGTCCAGATGATGAAAGTTCCCTTGCATTGAAGGCAACGGAGAGTTCCATATACGATTGGCTTAAGAACCAAGACGGGATCTCTCTCTCTCAGATTTTGGAGCACTTCAGCGGATCTGAAGAACAATCTGTGATCGATGTACTTAGCTCCCTTGAAGTTGACTTTCTAATATATAAAAAGAACAACTTGTATATGATTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

914

Amino Acids

101.8

Weight (kDa)

6.31

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 15 - 176 2.3e-24 DEAD/DEAH box helicase
Helicase_C PF00271 220 - 323 5.5e-22 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 335 - 397 3.6e-15 RecQ zinc-binding
RQC PF09382 400 - 497 1.2e-23 RQC domain
HRDC PF00570 547 - 613 4.7e-14 HRDC domain
HTH_40 PF14493 655 - 750 4e-17 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 718
AccB1I GGYRCC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 1352
AccBSI CCGCTC 1 cut(s) 1700
AccI GTMKAC 1 cut(s) 1287
AciI CCGC 6 cut(s) 117, 803, 957, 1170, 1698, 2649
AclWI GGATC 6 cut(s) 1512, 1841, 1851, 2111, 2624, 2659
AcoI YGGCCR 1 cut(s) 105
AcuI CTGAAG 3 cut(s) 2511, 2629, 2676
AcyI GRCGYC 1 cut(s) 1788
AfaI GTAC 6 cut(s) 50, 825, 1097, 1890, 1926, 2679
AfiI CCNNNNNNNGG 5 cut(s) 328, 1200, 1352, 1767, 2466
AflII CTTAAG 1 cut(s) 2600
AflIII ACRYGT 1 cut(s) 1212
AjnI CCWGG 5 cut(s) 939, 1967, 2020, 2250, 2465
Alw21I GWGCWC 2 cut(s) 651, 2643
Alw26I GTCTC 5 cut(s) 1203, 1277, 1414, 1424, 2280
AlwI GGATC 6 cut(s) 1512, 1841, 1851, 2111, 2624, 2659
Ama87I CYCGRG 2 cut(s) 1305, 2428
AoxI GGCC 5 cut(s) 105, 424, 469, 2176, 2406
ApeKI GCWGC 3 cut(s) 114, 1085, 2075
Asp700I GAANNNNTTC 3 cut(s) 522, 1152, 1227
AspLEI GCGC 1 cut(s) 678
AspS9I GGNCC 5 cut(s) 357, 424, 1499, 2177, 2407
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 1730, 2343
AsuII TTCGAA 1 cut(s) 1376
AsuNHI GCTAGC 1 cut(s) 1771
AvaI CYCGRG 2 cut(s) 1305, 2428
AvaII GGWCC 2 cut(s) 357, 1499
BalI TGGCCA 1 cut(s) 107
BanI GGYRCC 1 cut(s) 139
BanII GRGCYC 2 cut(s) 182, 651
BarI GAAGNNNNNNTAC 2 cut(s) 1278, 1310
BauI CACGAG 1 cut(s) 798
Bbv12I GWGCWC 2 cut(s) 651, 2643
BbvI GCAGC 3 cut(s) 126, 1097, 2062
BccI CCATC 9 cut(s) 97, 523, 677, 959, 1075, 1762, 1778, 1868, 2299
BciT130I CCWGG 5 cut(s) 941, 1969, 2022, 2252, 2467
BclI TGATCA 1 cut(s) 1720
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 5 cut(s) 1203, 1277, 1414, 1424, 2280
BfaI CTAG 4 cut(s) 455, 999, 1772, 2295
BfmI CTRYAG 1 cut(s) 1284
BfrI CTTAAG 1 cut(s) 2600
BfuAI ACCTGC 1 cut(s) 718
BglII AGATCT 1 cut(s) 2027
BisI GCNGC 4 cut(s) 115, 118, 1086, 2076
BlsI GCNGC 4 cut(s) 116, 119, 1087, 2077
Bme1390I CCNGG 6 cut(s) 323, 941, 1969, 2022, 2252, 2467
Bme18I GGWCC 2 cut(s) 357, 1499
BmeT110I CYCGRG 2 cut(s) 1305, 2428
BmgT120I GGNCC 5 cut(s) 357, 424, 1499, 2177, 2407
BmiI GGNNCC 4 cut(s) 141, 342, 425, 1696
BmrFI CCNGG 6 cut(s) 323, 941, 1969, 2022, 2252, 2467
BmsI GCATC 7 cut(s) 543, 944, 983, 1315, 1580, 2078, 2296
BmtI GCTAGC 1 cut(s) 1775
BplI GAGNNNNNCTC 4 cut(s) 819, 851, 1979, 2011
Bpu14I TTCGAA 1 cut(s) 1376
BpuEI CTTGAG 2 cut(s) 728, 2064
BpuMI CCSGG 1 cut(s) 323
Bsa29I ATCGAT 2 cut(s) 1782, 2673
BsaAI YACGTR 1 cut(s) 467
BsaHI GRCGYC 1 cut(s) 1788
BsaI GGTCTC 1 cut(s) 1277
BsaJI CCNNGG 7 cut(s) 321, 1393, 1690, 2020, 2376, 2403, 2466
BsaWI WCCGGW 2 cut(s) 588, 1501
Bsc4I CCNNNNNNNGG 5 cut(s) 328, 1200, 1352, 1767, 2466
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 3 cut(s) 169, 1529, 1536
Bse3DI GCAATG 2 cut(s) 406, 2262
BseBI CCWGG 5 cut(s) 941, 1969, 2022, 2252, 2467
BseCI ATCGAT 2 cut(s) 1782, 2673
BseDI CCNNGG 7 cut(s) 321, 1393, 1690, 2020, 2376, 2403, 2466
BseGI GGATG 3 cut(s) 534, 1879, 2093
BseLI CCNNNNNNNGG 5 cut(s) 328, 1200, 1352, 1767, 2466
BseMI GCAATG 2 cut(s) 406, 2262
BseMII CTCAG 5 cut(s) 693, 1288, 1622, 1641, 2642
BseNI ACTGG 3 cut(s) 169, 1529, 1536
BseRI GAGGAG 1 cut(s) 2442
BseXI GCAGC 3 cut(s) 126, 1097, 2062
BsgI GTGCAG 3 cut(s) 688, 1171, 2016
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 5 cut(s) 107, 426, 471, 2178, 2408
BshNI GGYRCC 1 cut(s) 139
BshVI ATCGAT 2 cut(s) 1782, 2673
BsiEI CGRYCG 1 cut(s) 47
BsiHKAI GWGCWC 2 cut(s) 651, 2643
BsiHKCI CYCGRG 2 cut(s) 1305, 2428
BsiSI CCGG 4 cut(s) 111, 323, 589, 1502
BslFI GGGAC 3 cut(s) 793, 2299, 2484
BslI CCNNNNNNNGG 5 cut(s) 328, 1200, 1352, 1767, 2466
BsmAI GTCTC 5 cut(s) 1203, 1277, 1414, 1424, 2280
BsmFI GGGAC 3 cut(s) 793, 2299, 2484
BsmI GAATGC 1 cut(s) 1245
BsnI GGCC 5 cut(s) 107, 426, 471, 2178, 2408
Bso31I GGTCTC 1 cut(s) 1277
BsoBI CYCGRG 2 cut(s) 1305, 2428
Bsp119I TTCGAA 1 cut(s) 1376
Bsp1286I GDGCHC 3 cut(s) 182, 651, 2643
BspACI CCGC 6 cut(s) 117, 803, 957, 1170, 1698, 2649
BspANI GGCC 5 cut(s) 107, 426, 471, 2178, 2408
BspCNI CTCAG 5 cut(s) 692, 1287, 1621, 1640, 2641
BspDI ATCGAT 2 cut(s) 1782, 2673
BspLI GGNNCC 4 cut(s) 141, 342, 425, 1696
BspMI ACCTGC 1 cut(s) 718
BspOI GCTAGC 1 cut(s) 1775
BspPI GGATC 6 cut(s) 1512, 1841, 1851, 2111, 2624, 2659
BspT104I TTCGAA 1 cut(s) 1376
BspT107I GGYRCC 1 cut(s) 139
BspTI CTTAAG 1 cut(s) 2600
BspTNI GGTCTC 1 cut(s) 1277
BsrBI CCGCTC 1 cut(s) 1700
BsrDI GCAATG 2 cut(s) 406, 2262
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 3 cut(s) 169, 1529, 1536
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 7 cut(s) 321, 1393, 1690, 2020, 2376, 2403, 2466
BssNI GRCGYC 1 cut(s) 1788
BssSI CACGAG 1 cut(s) 798
BssT1I CCWWGG 3 cut(s) 1393, 1690, 2403
Bst2BI CACGAG 1 cut(s) 798
Bst2UI CCWGG 5 cut(s) 941, 1969, 2022, 2252, 2467
Bst4CI ACNGT 7 cut(s) 48, 274, 1095, 1435, 1456, 1888, 2053
Bst6I CTCTTC 3 cut(s) 1417, 1659, 2460
BstACI GRCGYC 1 cut(s) 1788
BstAFI CTTAAG 1 cut(s) 2600
BstBAI YACGTR 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 1376
BstC8I GCNNGC 6 cut(s) 986, 1243, 1680, 1773, 1953, 2425
BstDEI CTNAG 9 cut(s) 368, 679, 1274, 1463, 1487, 1608, 1627, 2628, 2681
BstDSI CCRYGG 1 cut(s) 2376
BstEII GGTNACC 1 cut(s) 1397
BstENI CCTNNNNNAGG 1 cut(s) 2464
BstF5I GGATG 3 cut(s) 534, 1879, 2093
BstHHI GCGC 1 cut(s) 678
BstMAI GTCTC 5 cut(s) 1203, 1277, 1414, 1424, 2280
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 8 cut(s) 503, 857, 921, 1348, 1706, 1772, 2315, 2570
BstNI CCWGG 5 cut(s) 941, 1969, 2022, 2252, 2467
BstNSI RCATGY 5 cut(s) 336, 1216, 1571, 1657, 1985
BstPI GGTNACC 1 cut(s) 1397
BstSCI CCNGG 6 cut(s) 321, 939, 1967, 2020, 2250, 2465
BstSFI CTRYAG 1 cut(s) 1284
BstV1I GCAGC 3 cut(s) 126, 1097, 2062
BstX2I RGATCY 4 cut(s) 1843, 2027, 2616, 2651
BstXI CCANNNNNNTGG 3 cut(s) 947, 1543, 2534
BstYI RGATCY 4 cut(s) 1843, 2027, 2616, 2651
Bsu15I ATCGAT 2 cut(s) 1782, 2673
BsuRI GGCC 5 cut(s) 107, 426, 471, 2178, 2408
BsuTUI ATCGAT 2 cut(s) 1782, 2673
BtgI CCRYGG 1 cut(s) 2376
BtsCI GGATG 3 cut(s) 534, 1879, 2093
BtsI GCAGTG 1 cut(s) 1346
BtsIMutI CAGTG 5 cut(s) 270, 1346, 1536, 1543, 2162
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 6 cut(s) 986, 1243, 1680, 1773, 1953, 2425
CfoI GCGC 1 cut(s) 678
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 5 cut(s) 357, 424, 1499, 2177, 2407
ClaI ATCGAT 2 cut(s) 1782, 2673
CseI GACGC 1 cut(s) 1777
Csp6I GTAC 6 cut(s) 49, 824, 1096, 1889, 1925, 2678
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviQI GTAC 6 cut(s) 49, 824, 1096, 1889, 1925, 2678
DdeI CTNAG 9 cut(s) 368, 679, 1274, 1463, 1487, 1608, 1627, 2628, 2681
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 3 cut(s) 1417, 1659, 2460
EarI CTCTTC 3 cut(s) 1417, 1659, 2460
Ecl136II GAGCTC 1 cut(s) 649
Eco130I CCWWGG 3 cut(s) 1393, 1690, 2403
Eco24I GRGCYC 2 cut(s) 182, 651
Eco31I GGTCTC 1 cut(s) 1277
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 357, 1499
Eco53kI GAGCTC 1 cut(s) 649
Eco57I CTGAAG 3 cut(s) 2511, 2629, 2676
Eco88I CYCGRG 2 cut(s) 1305, 2428
Eco91I GGTNACC 1 cut(s) 1397
EcoICRI GAGCTC 1 cut(s) 649
EcoNI CCTNNNNNAGG 1 cut(s) 2464
EcoO65I GGTNACC 1 cut(s) 1397
EcoRII CCWGG 5 cut(s) 939, 1967, 2020, 2250, 2465
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 3 cut(s) 1393, 1690, 2403
EcoT22I ATGCAT 1 cut(s) 1573
EcoT38I GRGCYC 2 cut(s) 182, 651
ErhI CCWWGG 3 cut(s) 1393, 1690, 2403
FalI AAGNNNNNCTT 6 cut(s) 1215, 1247, 2373, 2405, 2708, 2740
FaqI GGGAC 3 cut(s) 793, 2299, 2484
FauNDI CATATG 2 cut(s) 565, 1705
FbaI TGATCA 1 cut(s) 1720
FblI GTMKAC 1 cut(s) 1287
Fnu4HI GCNGC 4 cut(s) 115, 118, 1086, 2076
FokI GGATG 3 cut(s) 541, 1886, 2100
FriOI GRGCYC 2 cut(s) 182, 651
Fsp4HI GCNGC 4 cut(s) 115, 118, 1086, 2076
FspBI CTAG 4 cut(s) 455, 999, 1772, 2295
GlaI GCGC 1 cut(s) 677
GluI GCNGC 4 cut(s) 115, 118, 1086, 2076
HaeIII GGCC 5 cut(s) 107, 426, 471, 2178, 2408
HapII CCGG 4 cut(s) 111, 323, 589, 1502
HgaI GACGC 1 cut(s) 1777
HhaI GCGC 1 cut(s) 678
Hin1I GRCGYC 1 cut(s) 1788
Hin6I GCGC 1 cut(s) 676
HinP1I GCGC 1 cut(s) 676
HincII GTYRAC 5 cut(s) 1055, 1792, 1947, 2324, 2698
HindII GTYRAC 5 cut(s) 1055, 1792, 1947, 2324, 2698
HindIII AAGCTT 3 cut(s) 1340, 1473, 2264
HpaI GTTAAC 1 cut(s) 1947
HpaII CCGG 4 cut(s) 111, 323, 589, 1502
HphI GGTGA 2 cut(s) 1730, 2343
Hpy166II GTNNAC 8 cut(s) 950, 1055, 1288, 1792, 1947, 2324, 2482, 2698
Hpy8I GTNNAC 8 cut(s) 950, 1055, 1288, 1792, 1947, 2324, 2482, 2698
HpyAV CCTTC 9 cut(s) 49, 354, 482, 1113, 1613, 1665, 2078, 2276, 2563
HpyCH4III ACNGT 7 cut(s) 48, 274, 1095, 1435, 1456, 1888, 2053
HpyCH4IV ACGT 2 cut(s) 466, 1057
HpyF10VI GCNNNNNNNGC 8 cut(s) 503, 857, 921, 1348, 1706, 1772, 2315, 2570
HpyF3I CTNAG 9 cut(s) 368, 679, 1274, 1463, 1487, 1608, 1627, 2628, 2681
HpySE526I ACGT 2 cut(s) 466, 1057
Hsp92I GRCGYC 1 cut(s) 1788
HspAI GCGC 1 cut(s) 676
Ksp22I TGATCA 1 cut(s) 1720
KspAI GTTAAC 1 cut(s) 1947
LmnI GCTCC 5 cut(s) 646, 1204, 1705, 2638, 2690
Lsp1109I GCAGC 3 cut(s) 126, 1097, 2062
LweI GCATC 7 cut(s) 543, 944, 983, 1315, 1580, 2078, 2296
MaeI CTAG 4 cut(s) 455, 999, 1772, 2295
MaeII ACGT 2 cut(s) 466, 1057
MaeIII GTNAC 8 cut(s) 462, 738, 1004, 1136, 1397, 1531, 1807, 2349
MbiI CCGCTC 1 cut(s) 1700
MfeI CAATTG 1 cut(s) 855
MflI RGATCY 4 cut(s) 1843, 2027, 2616, 2651
MhlI GDGCHC 3 cut(s) 182, 651, 2643
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 8 cut(s) 83, 372, 746, 815, 938, 1082, 1804, 2281
MmeI TCCRAC 4 cut(s) 136, 355, 1564, 2104
Mox20I TGGCCA 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 1573
MroXI GAANNNNTTC 3 cut(s) 522, 1152, 1227
MscI TGGCCA 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 3 cut(s) 117, 1160, 2649
MspCI CTTAAG 1 cut(s) 2600
MspI CCGG 4 cut(s) 111, 323, 589, 1502
MspR9I CCNGG 6 cut(s) 323, 941, 1969, 2022, 2252, 2467
MunI CAATTG 1 cut(s) 855
Mva1269I GAATGC 1 cut(s) 1245
MvaI CCWGG 5 cut(s) 941, 1969, 2022, 2252, 2467
MwoI GCNNNNNNNGC 8 cut(s) 503, 857, 921, 1348, 1706, 1772, 2315, 2570
NciI CCSGG 1 cut(s) 323
NdeI CATATG 2 cut(s) 565, 1705
NheI GCTAGC 1 cut(s) 1771
NlaIV GGNNCC 4 cut(s) 141, 342, 425, 1696
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 6 cut(s) 738, 1004, 1136, 1531, 1807, 2349
NsiI ATGCAT 1 cut(s) 1573
NspI RCATGY 5 cut(s) 336, 1216, 1571, 1657, 1985
NspV TTCGAA 1 cut(s) 1376
PaeR7I CTCGAG 1 cut(s) 1305
PciI ACATGT 1 cut(s) 1212
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 1303
PctI GAATGC 1 cut(s) 1245
PdmI GAANNNNTTC 3 cut(s) 522, 1152, 1227
PfeI GAWTC 6 cut(s) 266, 768, 1623, 1999, 2345, 2414
PflMI CCANNNNNTGG 1 cut(s) 1352
PkrI GCNGC 4 cut(s) 116, 119, 1087, 2077
PleI GAGTC 8 cut(s) 83, 372, 745, 814, 937, 1081, 1804, 2281
PpsI GAGTC 8 cut(s) 83, 372, 745, 814, 937, 1081, 1804, 2281
Ppu21I YACGTR 1 cut(s) 467
PscI ACATGT 1 cut(s) 1212
Psp124BI GAGCTC 1 cut(s) 651
Psp6I CCWGG 5 cut(s) 939, 1967, 2020, 2250, 2465
PspEI GGTNACC 1 cut(s) 1397
PspGI CCWGG 5 cut(s) 939, 1967, 2020, 2250, 2465
PspN4I GGNNCC 4 cut(s) 141, 342, 425, 1696
PspPI GGNCC 5 cut(s) 357, 424, 1499, 2177, 2407
PsuI RGATCY 4 cut(s) 1843, 2027, 2616, 2651
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 6 cut(s) 50, 825, 1097, 1890, 1926, 2679
RsaNI GTAC 6 cut(s) 49, 824, 1096, 1889, 1925, 2678
SacI GAGCTC 1 cut(s) 651
SatI GCNGC 4 cut(s) 115, 118, 1086, 2076
Sau96I GGNCC 5 cut(s) 357, 424, 1499, 2177, 2407
SchI GAGTC 8 cut(s) 83, 372, 746, 815, 938, 1082, 1804, 2281
ScrFI CCNGG 6 cut(s) 323, 941, 1969, 2022, 2252, 2467
SduI GDGCHC 3 cut(s) 182, 651, 2643
SfaNI GCATC 7 cut(s) 543, 944, 983, 1315, 1580, 2078, 2296
SfcI CTRYAG 1 cut(s) 1284
Sfr274I CTCGAG 1 cut(s) 1305
SfuI TTCGAA 1 cut(s) 1376
SinI GGWCC 2 cut(s) 357, 1499
SlaI CTCGAG 1 cut(s) 1305
SmlI CTYRAG 5 cut(s) 14, 743, 1305, 2079, 2600
SmoI CTYRAG 5 cut(s) 14, 743, 1305, 2079, 2600
SsiI CCGC 6 cut(s) 117, 803, 957, 1170, 1698, 2649
SspI AATATT 1 cut(s) 23
SspMI CTAG 4 cut(s) 455, 999, 1772, 2295
SstI GAGCTC 1 cut(s) 651
StyD4I CCNGG 6 cut(s) 321, 939, 1967, 2020, 2250, 2465
StyI CCWWGG 3 cut(s) 1393, 1690, 2403
TaaI ACNGT 7 cut(s) 48, 274, 1095, 1435, 1456, 1888, 2053
TaiI ACGT 2 cut(s) 469, 1060
TatI WGTACW 1 cut(s) 2677
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 6 cut(s) 266, 768, 1623, 1999, 2345, 2414
TscAI CASTG 5 cut(s) 277, 1353, 1536, 1543, 2162
TseFI GTSAC 6 cut(s) 738, 1004, 1136, 1531, 1807, 2349
TseI GCWGC 3 cut(s) 114, 1085, 2075
Tsp45I GTSAC 6 cut(s) 738, 1004, 1136, 1531, 1807, 2349
TspDTI ATGAA 5 cut(s) 414, 846, 1857, 2454, 2565
TspGWI ACGGA 5 cut(s) 96, 135, 2198, 2393, 2591
TspRI CASTG 5 cut(s) 277, 1353, 1536, 1543, 2162
Van91I CCANNNNNTGG 1 cut(s) 1352
Vha464I CTTAAG 1 cut(s) 2600
VpaK11BI GGWCC 2 cut(s) 357, 1499
XagI CCTNNNNNAGG 1 cut(s) 2464
XceI RCATGY 5 cut(s) 336, 1216, 1571, 1657, 1985
XhoI CTCGAG 1 cut(s) 1305
XmiI GTMKAC 1 cut(s) 1287
XmnI GAANNNNTTC 3 cut(s) 522, 1152, 1227
XspI CTAG 4 cut(s) 455, 999, 1772, 2295
Zsp2I ATGCAT 1 cut(s) 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.