Rw5G045150

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003692
Physical Location & Seq
Forward (+)
78988825 .. 78996923
8099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045150.1

Sequence Viewer

Length: 2820 bp
ATGGCGGGAGATCGAGGTTGCCCTTTTACAGAGCGTCTCAACGGCAGAGCAACCAGAAACCTCAAAATGCAGTCTATGCAGGCCATTCTCAAGCAATATTTTGGGTTTTCTTCATTTCGGCCATACCAAGAGGAAGTGATTGAGAAAATCATAGCAGGAAGGGACTCTTTGATTGTCATGGCCACTGGAAGTGGCAAGTCTTTGTGCTATCAAGTGCCTCCTTTGGTTGTTGGAAAGACAGGTGTGGTTGTGAGTCCTCTTATATCCCTAATGCAAGATCAGGTAATGTCTTTGAAACAAAGAGGGATCAGAGCCGAGTATATGGGTAGTAGTCAATCGGATAACACTGTCCAGAGCCGAGCCGAGAGTGGTCAATTTGATATCTTGTACATGACCCCTGAAAAGGCATGTGTGATTCCTGTCAGCTTCTGGTCAAAATTACTAAGGGCTGGGATCTGCTTGTTTGCTGTTGATGAAGCACACTGCATATCGGAGTGGGGCCATGATTTCAGGGTGGAATACAAGCAATTGGACAAGTTACGTGCCATTCTTGTTGGTGTCCCATTTATTGGCTTAACTGCAACTGCTACTGAAAAGGTTCGAATGGACATTGTTAATTCCTTGAAGATGAGAAACCCATATGTCAAAATAGGTTCATTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTCATGAGCTGGTTCAAGAAGTTTCCAAATTTGTACGCACAGATGGTTCAACGATAATATACTGCACGACAATTAAAGACGTTGATCAGGTATTCAATTCACTGAAGGAGGTGGGTATTAAAGCTGGAATATATCATGGTCAAATGGATAATAAAGCTCGTGCCGAATCCCATAGATTATTTATAAGAGATGAACTAGATGTCATGGTTGCTACTATTGCTTTTGGAATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCACTATGGCTGCCCGAAGAGTTTGGAGTCTTATTACCAGGAAAGTGGACGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACTTCACAAAAGCTGACTTTTATGCTGGAGAGTGTCATACAGAAAGTCAACGAAGAGCTGTTGTAGAGTCATTGATGGCAGCACAACAGTATTGCTTATCAACAACTTGCAGAAGGAAGTTGTTGCTTGGTCACTTTGGGGAAATCTTTCCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCCTCAAAGAAGGAGAGGGACATGTCTAGAGAAGCATTTCTTCTCATGGCTTGCATTCAATCATGTCGGGGTAAATGGGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCACGGGCCAAAAAGATTCTTGATGCCCAATATGACAAGCTTCCACTGCATGGACTCGGGAAAGACTATTCAGCAAATTGGTGGAAAGCACTTTCTTACCAATTAATCTCTTCTGGTTATTTGATGGAGACGGTATCTGATATATACAGAACTGTAAGTGTCAGTCGGAAAGGGGAGCAATTTCTGAGTTCTGCTGGACCTGATCATCAACCGCCTTTAGTTTTGCCAGTGACCAGTGAAATGGTAGATGATGAGGACAATAAAAGTACATTAGGTGAAGTTGGAGAAATTAAAAGTTTGTCTACTCTGGAATGCGAAGGATTTTCGGAGGCTGAGAAGCAACTGTATCACTCACTTCTTGAAGAGAGAAGGAAGCTTGCAAGAAGCCTTGGAACTGCTCCATATGCCATTTGTGGTGATCAAGCAATCAAAAAAATTGCTTTAACAAGACCATCTACCAGAGCAAGGTTAGCTAACATCGATGGCGTCAACCAGCACCTTGTAGTGGCACACGGTAATCATTTTCTTCAAATCATTCGGCATCTATCACAAGGACTAAACCTTTCATTGGATGGAGAGGCACCCATACAAACTGCTATTATGAAGAAAGTATATCCAGTACCTAACCACCAACAGAGAAAGTTAACACCGGCCAAGTTTGAGGCTTGGAGAATGTGGCATGAAGACGGCCTCTCAATTCAGAAAATTGCTAATTTCCCTGGTAGAGCTGCTCCTATTAAAGAGCAGACTGTTCAAGAATATCTGATGGAAGCTGCTCAAGAAGGATTTGCAATTGATTGGGTCAGATTATGCGATGAGGTTGGACTGACACAAAAAGTTATCTCAGATATTCAGTGTGCCATTTCAAAGGTTGGCTCTAGAGAAAAGTTAAAGCCTATCAAAGATGAATTACCAGAAGATATAAGTTATGGACGCATCAAGACTTTCCTGACAATGGAAAAGTGTGGGATCTCGCTGGATGGGCCTTTACCTAACCACCACAATGCAGGAAAAGATGATCAACTCCTCAAGAAGGAAACTGAATTGTCACCGAGTTCTATTCATACATCCCCTATGGAAGAACCTCATGAAGTTAAAACCTCAGGCCGAGATTCAGATGCTTACAGCTTGGATAAAAATAAAGAAACAGCTTCCCTTCCATTCAATAGAGAACAAGGTTTAAAGCTACCTGAAGTACATTTTGAGGATTTACTCTCAAAGAAACGCCAAAAGCTTGGTAGTCCCAAGGAGGAGAGTAAAACTGCACTGAAGGCAACCGAGAATTCCATATTGGATTGGCTTAGTACCAAAAATGGAGATACTCTCTCTGCGATTCTGGAGCACTTCAATGGATCTGAAGAAGACTGTGTCATCGAACTCCTCACTGGCCTTGAAAGTGACTTTATGATATTCAGAAAGAACAATATGTATTTCGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

939

Amino Acids

105.51

Weight (kDa)

7.71

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 38 - 196 2.6e-06 Type III restriction enzyme, res subunit
DEAD PF00270 40 - 201 2.2e-24 DEAD/DEAH box helicase
Helicase_C PF00271 249 - 348 1.3e-21 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 360 - 421 6.4e-14 RecQ zinc-binding
RQC PF09382 425 - 522 4.7e-24 RQC domain
HRDC PF00570 572 - 638 1.2e-13 HRDC domain
HTH_40 PF14493 680 - 775 2.9e-18 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 1957
AccB7I CCANNNNNTGG 2 cut(s) 569, 1427
AccI GTMKAC 1 cut(s) 1679
AciI CCGC 2 cut(s) 5, 1589
AclWI GGATC 4 cut(s) 314, 461, 2354, 2737
AcoI YGGCCR 3 cut(s) 119, 180, 2028
AcsI RAATTY 2 cut(s) 743, 2659
AcuI CTGAAG 4 cut(s) 839, 2589, 2666, 2754
AcyI GRCGYC 1 cut(s) 1863
AfaI GTAC 6 cut(s) 389, 750, 1645, 1998, 2574, 2683
AfiI CCNNNNNNNGG 8 cut(s) 403, 569, 1427, 1842, 1882, 1945, 2332, 2410
AflIII ACRYGT 1 cut(s) 1287
AjnI CCWGG 2 cut(s) 1014, 2095
AjuI GAANNNNNNNTTGG 2 cut(s) 2651, 2683
Alw21I GWGCWC 1 cut(s) 2721
Alw26I GTCTC 2 cut(s) 41, 1499
AlwI GGATC 4 cut(s) 314, 461, 2354, 2737
AlwNI CAGNNNCTG 1 cut(s) 429
Ama87I CYCGRG 1 cut(s) 1433
ApeKI GCWGC 4 cut(s) 987, 1160, 2105, 2150
ApoI RAATTY 2 cut(s) 743, 2659
AseI ATTAAT 1 cut(s) 1481
Asp700I GAANNNNTTC 3 cut(s) 597, 1227, 1302
AspS9I GGNCC 4 cut(s) 499, 1383, 1574, 2360
AsuHPI GGTGA 3 cut(s) 1664, 1805, 2418
AsuII TTCGAA 1 cut(s) 601
AvaI CYCGRG 1 cut(s) 1433
AvaII GGWCC 1 cut(s) 1574
AxyI CCTNAGG 1 cut(s) 2479
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 1957
BarI GAAGNNNNNNTAC 2 cut(s) 1353, 1385
BauI CACGAG 1 cut(s) 873
BbsI GAAGAC 2 cut(s) 2067, 2745
Bbv12I GWGCWC 1 cut(s) 2721
BbvI GCAGC 4 cut(s) 974, 1172, 2092, 2137
BceAI ACGGC 2 cut(s) 58, 2080
BciT130I CCWGG 2 cut(s) 1016, 2097
BclI TGATCA 4 cut(s) 799, 1579, 1795, 2395
BcoDI GTCTC 2 cut(s) 41, 1499
BfaI CTAG 3 cut(s) 911, 1293, 2256
BfmI CTRYAG 1 cut(s) 1359
BisI GCNGC 4 cut(s) 988, 1161, 2106, 2151
BlsI GCNGC 4 cut(s) 989, 1162, 2107, 2152
Bme1390I CCNGG 2 cut(s) 1016, 2097
Bme18I GGWCC 1 cut(s) 1574
BmeT110I CYCGRG 1 cut(s) 1433
BmgT120I GGNCC 4 cut(s) 499, 1383, 1574, 2360
BmiI GGNNCC 2 cut(s) 500, 1959
BmrFI CCNGG 2 cut(s) 1016, 2097
BmsI GCATC 4 cut(s) 1390, 1927, 2322, 2485
BpiI GAAGAC 2 cut(s) 2067, 2745
BplI GAGNNNNNCTC 2 cut(s) 2685, 2717
BpmI CTGGAG 2 cut(s) 1128, 2735
Bpu14I TTCGAA 1 cut(s) 601
BpuEI CTTGAG 3 cut(s) 74, 2139, 2390
Bsa29I ATCGAT 1 cut(s) 1857
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 1863
BsaJI CCNNGG 3 cut(s) 1765, 2095, 2622
Bsc4I CCNNNNNNNGG 8 cut(s) 403, 569, 1427, 1842, 1882, 1945, 2332, 2410
Bse118I RCCGGY 1 cut(s) 2026
Bse1I ACTGG 5 cut(s) 190, 1604, 1611, 1994, 2767
Bse21I CCTNAGG 1 cut(s) 2479
BseBI CCWGG 2 cut(s) 1016, 2097
BseCI ATCGAT 1 cut(s) 1857
BseDI CCNNGG 3 cut(s) 1765, 2095, 2622
BseGI GGATG 3 cut(s) 1954, 2362, 2444
BseLI CCNNNNNNNGG 8 cut(s) 403, 569, 1427, 1842, 1882, 1945, 2332, 2410
BseMII CTCAG 4 cut(s) 1553, 1701, 2235, 2493
BseNI ACTGG 5 cut(s) 190, 1604, 1611, 1994, 2767
BseRI GAGGAG 3 cut(s) 2393, 2642, 2747
BseXI GCAGC 4 cut(s) 974, 1172, 2092, 2137
BseYI CCCAGC 1 cut(s) 449
BsgI GTGCAG 2 cut(s) 763, 2625
BshNI GGYRCC 1 cut(s) 1957
BshVI ATCGAT 1 cut(s) 1857
BsiHKAI GWGCWC 1 cut(s) 2721
BsiHKCI CYCGRG 1 cut(s) 1433
BsiSI CCGG 1 cut(s) 2027
BslFI GGGAC 4 cut(s) 176, 545, 1298, 2604
BslI CCNNNNNNNGG 8 cut(s) 403, 569, 1427, 1842, 1882, 1945, 2332, 2410
BsmAI GTCTC 2 cut(s) 41, 1499
BsmBI CGTCTC 2 cut(s) 41, 1499
BsmFI GGGAC 4 cut(s) 176, 545, 1298, 2604
BsmI GAATGC 2 cut(s) 1320, 1694
BsoBI CYCGRG 1 cut(s) 1433
Bsp119I TTCGAA 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 2721
Bsp1407I TGTACA 1 cut(s) 387
BspACI CCGC 2 cut(s) 5, 1589
BspCNI CTCAG 4 cut(s) 1554, 1702, 2234, 2492
BspDI ATCGAT 1 cut(s) 1857
BspHI TCATGA 2 cut(s) 718, 2464
BspLI GGNNCC 2 cut(s) 500, 1959
BspPI GGATC 4 cut(s) 314, 461, 2354, 2737
BspQI GCTCTTC 1 cut(s) 1129
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 1957
BsrFI RCCGGY 1 cut(s) 2026
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 5 cut(s) 190, 1604, 1611, 1994, 2767
BssAI RCCGGY 1 cut(s) 2026
BssECI CCNNGG 3 cut(s) 1765, 2095, 2622
BssNI GRCGYC 1 cut(s) 1863
BssSI CACGAG 1 cut(s) 873
BssT1I CCWWGG 2 cut(s) 1765, 2622
Bst2BI CACGAG 1 cut(s) 873
Bst2UI CCWGG 2 cut(s) 1016, 2097
Bst4CI ACNGT 8 cut(s) 349, 1170, 1510, 1531, 1722, 1892, 2128, 2744
Bst6I CTCTTC 4 cut(s) 989, 1129, 1492, 1734
BstACI GRCGYC 1 cut(s) 1863
BstAPI GCANNNNNTGC 1 cut(s) 76
BstAUI TGTACA 1 cut(s) 387
BstBAI YACGTR 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 601
BstC8I GCNNGC 4 cut(s) 81, 1061, 1318, 1755
BstDEI CTNAG 6 cut(s) 443, 1562, 1710, 2221, 2479, 2678
BstENI CCTNNNNNAGG 1 cut(s) 2408
BstF5I GGATG 3 cut(s) 1954, 2362, 2444
BstMAI GTCTC 2 cut(s) 41, 1499
BstMWI GCNNNNNNNGC 7 cut(s) 76, 932, 1423, 1781, 1847, 2359, 2648
BstNI CCWGG 2 cut(s) 1016, 2097
BstNSI RCATGY 2 cut(s) 411, 1291
BstSCI CCNGG 2 cut(s) 1014, 2095
BstSFI CTRYAG 1 cut(s) 1359
BstV1I GCAGC 4 cut(s) 974, 1172, 2092, 2137
BstV2I GAAGAC 2 cut(s) 2067, 2745
BstX2I RGATCY 3 cut(s) 453, 2346, 2729
BstXI CCANNNNNNTGG 3 cut(s) 1022, 1618, 2612
BstYI RGATCY 3 cut(s) 453, 2346, 2729
Bsu15I ATCGAT 1 cut(s) 1857
Bsu36I CCTNAGG 1 cut(s) 2479
BsuTUI ATCGAT 1 cut(s) 1857
BtgZI GCGATG 1 cut(s) 2205
BtsCI GGATG 3 cut(s) 1954, 2362, 2444
BtsI GCAGTG 2 cut(s) 481, 1421
Cac8I GCNNGC 4 cut(s) 81, 1061, 1318, 1755
CaiI CAGNNNCTG 1 cut(s) 429
CciI TCATGA 2 cut(s) 718, 2464
Cfr10I RCCGGY 1 cut(s) 2026
Cfr13I GGNCC 4 cut(s) 499, 1383, 1574, 2360
ClaI ATCGAT 1 cut(s) 1857
CseI GACGC 3 cut(s) 23, 1852, 2319
Csp6I GTAC 6 cut(s) 388, 749, 1644, 1997, 2573, 2682
CviQI GTAC 6 cut(s) 388, 749, 1644, 1997, 2573, 2682
DdeI CTNAG 6 cut(s) 443, 1562, 1710, 2221, 2479, 2678
DraI TTTAAA 1 cut(s) 2559
EaeI YGGCCR 3 cut(s) 119, 180, 2028
Eam1104I CTCTTC 4 cut(s) 989, 1129, 1492, 1734
EarI CTCTTC 4 cut(s) 989, 1129, 1492, 1734
Eco130I CCWWGG 2 cut(s) 1765, 2622
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 1574
Eco57I CTGAAG 4 cut(s) 839, 2589, 2666, 2754
Eco81I CCTNAGG 1 cut(s) 2479
Eco88I CYCGRG 1 cut(s) 1433
EcoNI CCTNNNNNAGG 1 cut(s) 2408
EcoRI GAATTC 1 cut(s) 2659
EcoRII CCWGG 2 cut(s) 1014, 2095
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 2 cut(s) 1765, 2622
ErhI CCWWGG 2 cut(s) 1765, 2622
Esp3I CGTCTC 2 cut(s) 41, 1499
FalI AAGNNNNNCTT 4 cut(s) 151, 183, 1290, 1322
FaqI GGGAC 4 cut(s) 176, 545, 1298, 2604
FauNDI CATATG 2 cut(s) 640, 1780
FbaI TGATCA 4 cut(s) 799, 1579, 1795, 2395
FblI GTMKAC 1 cut(s) 1679
Fnu4HI GCNGC 4 cut(s) 988, 1161, 2106, 2151
FokI GGATG 3 cut(s) 1961, 2369, 2431
Fsp4HI GCNGC 4 cut(s) 988, 1161, 2106, 2151
FspBI CTAG 3 cut(s) 911, 1293, 2256
GluI GCNGC 4 cut(s) 988, 1161, 2106, 2151
GsaI CCCAGC 1 cut(s) 453
GsuI CTGGAG 2 cut(s) 1128, 2735
HapII CCGG 1 cut(s) 2027
HgaI GACGC 3 cut(s) 23, 1852, 2319
Hin1I GRCGYC 1 cut(s) 1863
HincII GTYRAC 3 cut(s) 1130, 1867, 2022
HindII GTYRAC 3 cut(s) 1130, 1867, 2022
HindIII AAGCTT 3 cut(s) 1415, 1751, 2609
HpaI GTTAAC 1 cut(s) 2022
HpaII CCGG 1 cut(s) 2027
HphI GGTGA 3 cut(s) 1664, 1805, 2418
Hpy166II GTNNAC 5 cut(s) 1025, 1130, 1680, 1867, 2022
Hpy8I GTNNAC 5 cut(s) 1025, 1130, 1680, 1867, 2022
HpyCH4III ACNGT 8 cut(s) 349, 1170, 1510, 1531, 1722, 1892, 2128, 2744
HpyCH4IV ACGT 2 cut(s) 541, 795
HpyF10VI GCNNNNNNNGC 7 cut(s) 76, 932, 1423, 1781, 1847, 2359, 2648
HpyF3I CTNAG 6 cut(s) 443, 1562, 1710, 2221, 2479, 2678
HpySE526I ACGT 2 cut(s) 541, 795
Hsp92I GRCGYC 1 cut(s) 1863
Ksp22I TGATCA 4 cut(s) 799, 1579, 1795, 2395
KspAI GTTAAC 1 cut(s) 2022
LguI GCTCTTC 1 cut(s) 1129
LmnI GCTCC 4 cut(s) 1552, 1780, 2113, 2716
Lsp1109I GCAGC 4 cut(s) 974, 1172, 2092, 2137
LweI GCATC 4 cut(s) 1390, 1927, 2322, 2485
MaeI CTAG 3 cut(s) 911, 1293, 2256
MaeII ACGT 2 cut(s) 541, 795
MaeIII GTNAC 5 cut(s) 537, 1211, 1606, 2424, 2774
MfeI CAATTG 2 cut(s) 527, 2169
MflI RGATCY 3 cut(s) 453, 2346, 2729
MhlI GDGCHC 1 cut(s) 2721
MlsI TGGCCA 1 cut(s) 182
MluNI TGGCCA 1 cut(s) 182
MlyI GAGTC 6 cut(s) 158, 262, 697, 1013, 1157, 1425
MmeI TCCRAC 4 cut(s) 211, 1523, 1639, 2179
Mox20I TGGCCA 1 cut(s) 182
MroXI GAANNNNTTC 3 cut(s) 597, 1227, 1302
MscI TGGCCA 1 cut(s) 182
MslI CAYNNNNRTG 2 cut(s) 2379, 2724
Msp20I TGGCCA 1 cut(s) 182
MspI CCGG 1 cut(s) 2027
MspR9I CCNGG 2 cut(s) 1016, 2097
MunI CAATTG 2 cut(s) 527, 2169
Mva1269I GAATGC 2 cut(s) 1320, 1694
MvaI CCWGG 2 cut(s) 1016, 2097
MwoI GCNNNNNNNGC 7 cut(s) 76, 932, 1423, 1781, 1847, 2359, 2648
NdeI CATATG 2 cut(s) 640, 1780
NlaIV GGNNCC 2 cut(s) 500, 1959
NmeAIII GCCGAG 4 cut(s) 340, 383, 388, 2510
NmuCI GTSAC 4 cut(s) 1211, 1606, 2424, 2774
NspI RCATGY 2 cut(s) 411, 1291
NspV TTCGAA 1 cut(s) 601
PagI TCATGA 2 cut(s) 718, 2464
PciI ACATGT 1 cut(s) 1287
PciSI GCTCTTC 1 cut(s) 1129
PctI GAATGC 2 cut(s) 1320, 1694
PdmI GAANNNNTTC 3 cut(s) 597, 1227, 1302
PfeI GAWTC 5 cut(s) 415, 881, 1393, 2489, 2710
PflFI GACNNNGTC 1 cut(s) 2744
PflMI CCANNNNNTGG 2 cut(s) 569, 1427
PkrI GCNGC 4 cut(s) 989, 1162, 2107, 2152
PleI GAGTC 6 cut(s) 158, 261, 697, 1012, 1156, 1425
PpsI GAGTC 6 cut(s) 158, 261, 697, 1012, 1156, 1425
Ppu21I YACGTR 1 cut(s) 542
PscI ACATGT 1 cut(s) 1287
PshBI ATTAAT 1 cut(s) 1481
PsiI TTATAA 1 cut(s) 899
Psp6I CCWGG 2 cut(s) 1014, 2095
PspFI CCCAGC 1 cut(s) 449
PspGI CCWGG 2 cut(s) 1014, 2095
PspN4I GGNNCC 2 cut(s) 500, 1959
PspPI GGNCC 4 cut(s) 499, 1383, 1574, 2360
PstNI CAGNNNCTG 1 cut(s) 429
PsuI RGATCY 3 cut(s) 453, 2346, 2729
PsyI GACNNNGTC 1 cut(s) 2744
RsaI GTAC 6 cut(s) 389, 750, 1645, 1998, 2574, 2683
RsaNI GTAC 6 cut(s) 388, 749, 1644, 1997, 2573, 2682
RseI CAYNNNNRTG 2 cut(s) 2379, 2724
SapI GCTCTTC 1 cut(s) 1129
SatI GCNGC 4 cut(s) 988, 1161, 2106, 2151
Sau96I GGNCC 4 cut(s) 499, 1383, 1574, 2360
SchI GAGTC 6 cut(s) 158, 262, 697, 1013, 1157, 1425
ScrFI CCNGG 2 cut(s) 1016, 2097
SduI GDGCHC 1 cut(s) 2721
SfaNI GCATC 4 cut(s) 1390, 1927, 2322, 2485
SfcI CTRYAG 1 cut(s) 1359
SfuI TTCGAA 1 cut(s) 601
SinI GGWCC 1 cut(s) 1574
SmiMI CAYNNNNRTG 2 cut(s) 2379, 2724
SmlI CTYRAG 3 cut(s) 89, 2154, 2405
SmoI CTYRAG 3 cut(s) 89, 2154, 2405
SsiI CCGC 2 cut(s) 5, 1589
SspI AATATT 1 cut(s) 98
SspMI CTAG 3 cut(s) 911, 1293, 2256
StyD4I CCNGG 2 cut(s) 1014, 2095
StyI CCWWGG 2 cut(s) 1765, 2622
TaaI ACNGT 8 cut(s) 349, 1170, 1510, 1531, 1722, 1892, 2128, 2744
TaiI ACGT 2 cut(s) 544, 798
TaqI TCGA 4 cut(s) 13, 601, 1857, 2751
TatI WGTACW 3 cut(s) 387, 1643, 2572
TfiI GAWTC 5 cut(s) 415, 881, 1393, 2489, 2710
TseFI GTSAC 4 cut(s) 1211, 1606, 2424, 2774
TseI GCWGC 4 cut(s) 987, 1160, 2105, 2150
Tsp45I GTSAC 4 cut(s) 1211, 1606, 2424, 2774
Tth111I GACNNNGTC 1 cut(s) 2744
Van91I CCANNNNNTGG 2 cut(s) 569, 1427
VpaK11BI GGWCC 1 cut(s) 1574
VspI ATTAAT 1 cut(s) 1481
XagI CCTNNNNNAGG 1 cut(s) 2408
XapI RAATTY 2 cut(s) 743, 2659
XbaI TCTAGA 2 cut(s) 1292, 2255
XceI RCATGY 2 cut(s) 411, 1291
XmiI GTMKAC 1 cut(s) 1679
XmnI GAANNNNTTC 3 cut(s) 597, 1227, 1302
XspI CTAG 3 cut(s) 911, 1293, 2256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.