RLG00000036447

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003692
Physical Location & Seq
Forward (+)
80738282 .. 80746355
8074 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036447

Sequence Viewer

Length: 2655 bp
ATGCAGTCTATGCAGGCCATTCTCAAGCAATATTTTGGGTTTTCTTCATTTCGGCCATACCAAGAAGAAGTGATTGAGAAAATTATAGCAGGAAGGGACTCTTTGATTGTGATGGCCACTGGAAGTGGCAAGTCTTTATGCTATCAAGTGCCTCCTTTGGTTGTTGGAAAGACTGGTGTGGTTGTGAGTCCTCTTATATCCTTAATGCAAGATCAGGTAATGGCTTTGAAACAAAGAGGGATCAGAGCTGAGTATATGGGTAGTAGTCAAACTGATAGCACTGTCCAGAGCCGAGCTGAGAGTGGTCAATTTGATATCTTGTACATGACCCCTGAAAAGGCATGTGTGATTCCTGTCAGCTTCTGGTCAAAATTACTAAGGGCTGGGATCTGCTTGTTTGCTGTTGATGAAGCACACTGCATATCGGAGTGGGGCCATGATTTCAGGGTGGAATACAAGCAATTGGACAAGTTACGTGGCGTTCTTGTTGGTGTCCCATTTATTGGCTTAACTGCAACTGCTACTGAAAAGGTTCGAATGGACGTTGTTAATTCCTTGAAGATGGAAAACCCATATGTCAAAATAGGCTCATTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTTATGAGCTGGTTGAAGAAGTTTCCAAATTTGTACACACAGATGGTTCAACAATAATATACTGTACGACAATTAAAGATGTTGATCAGGTATTCAATTCACTGAAGGAGGTGGGCATTAAAGCTGGAATCTACCATGGTCAAATGGATAATAAAGCTCGTGCCGAATCCCATAGATTGTTTATAAGAGATGAACTGGATGTCATGGTTGCCACTATTGCTTTTGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCACTATGGCTGCCCGAAGAGTTTGGAGTCTTATTACCAGGAAAGTGGACGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACTTCACAAAAGCTGACTTTTATGCTGGAGAGTGTCAAACAGAAAGTCAACGAAGAGCTGTTGTAGAGTCATTGATGGCAGCACAACAGTATTGCTTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTCACTTTGGGGAAATCTTTCCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCTTCAAAGAAGGAGAGGGACATGTCTAGAGAAGCATTTCTTCTAATGGCTTGCATTCAATCATGTCGGGGTAAATGGGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCTCGGGCGAAAAAGATTCTTGATGCCCAATATGACAAGCTTCCACTGCATGGACTTGGGAAAGAGTATTCAGCAAATTGGTGGAAAGCACTGGCTTACCAATTAATCTCTTCTGGTTATTTGACGGAGACGGTATCTGATATATACAGGACTGTAAGTGTCAGTCGGAAAGGGGAGCAATTTCTAAGTTCTGCTGGACCTGATCATCAACCGCCTCTAGTTTTGCCAGTGACCAGTGAAATGGCAGATGATGAGGACAATAAAAGTACATCAGGTGAAGTTGGAGAAATTAAAAGTTTGTCTACTCTGGAATGCGAAGGATTTTCGGAGGCTGAGAAGCAACTGTATCACTCACTTCTTGAAAAGAGAAGGAAGCTTGCAAGAAGCCTTGGAACTCACCTTGTAGTGACACACGGTAATCATTTTCTTCAAATCATTCGGCATCTATCACAAGGACTAAACCTTTCATTGGATGGAGAGGCACCCATACAAACTGCTATTACGAAGAAAGTATATCCAGTACCTAACCACCAACAGAGAAAGTTAACACCGGCAAAGTTTGAAGCTTGGAGAATGTGGCATGAAGACAGCCTCTCAATTCAGAAAATTGCTAATTTCCCTGGTAGAGCTGCTCCTATTAAAGAGCAGACTGTTCAAGAATATCTGATGGAAGCTGCTCAAGAAGGATTTGCAATTGATTGGGTCAGATTATGCAATGAGGTTGGACTGACACAAAAAGCTATCTCAGATATTCAGTGTGCCATTTCAAAGGTTGGCTCTAGAGAAAAGTTAAAACCTATCAAGGACGAATTACCAGAAGATATAAGTTATGGACACATCAAGACTTTCCTGACAATGGAAAAGTGTGGGATCTCGCTGGATGGGCCTCTACCTAACCACCACAATGCAGGAAAAGATGATCAACTCCTCAAGAAGGAAACTGAATTGTCACCGAGTTCTATTCATACATCCCCTATGGAAGAACCTCATGAAGTTAAAACCTCAGGCCGAGATTCAGATGCTTACAGCTTGGATAAAGATAAAGAAACAGCTTCCCTTCCATTCAATAGAGAACAAGGTTTAAAGCTACCTGAAGTACATTTGGAGGATTCACTCTCAAAGAAACGCCAAAAGCTTGGTAGTCCCAAGGAGGAGAGTAAAACTACACTGAAGGCAACCGAGAATTCCATATTGGATTGGCTTAGTACCAAAAATGGAGTTACTCTCTCTGTGATTCTGGAGCACTTCAATGGATCTGAAGAAGACTGTGTCATCGAACTCCTCACTGGCCTTGAAAGTGACTTTATGATATACAGAAAGAACAATATGTATTTCGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

885

Amino Acids

99.38

Weight (kDa)

6.48

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 16 - 173 9.4e-06 Type III restriction enzyme, res subunit
DEAD PF00270 18 - 179 2.2e-24 DEAD/DEAH box helicase
Helicase_C PF00271 224 - 326 7.1e-22 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 338 - 399 4.6e-14 RecQ zinc-binding
RQC PF09382 403 - 500 1.7e-24 RQC domain
HTH_40 PF14493 625 - 720 1.4e-17 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 1792
AccB7I CCANNNNNTGG 2 cut(s) 503, 1361
AccI GTMKAC 1 cut(s) 1613
AciI CCGC 1 cut(s) 1523
AclWI GGATC 4 cut(s) 248, 395, 2189, 2572
AcoI YGGCCR 2 cut(s) 53, 114
AcsI RAATTY 2 cut(s) 677, 2494
AcuI CTGAAG 4 cut(s) 773, 2424, 2501, 2589
AfaI GTAC 7 cut(s) 323, 684, 715, 1579, 1833, 2409, 2518
AfiI CCNNNNNNNGG 6 cut(s) 337, 503, 1361, 1780, 2167, 2245
AflIII ACRYGT 1 cut(s) 1221
AjnI CCWGG 2 cut(s) 948, 1930
AjuI GAANNNNNNNTTGG 2 cut(s) 2486, 2518
Alw21I GWGCWC 1 cut(s) 2556
Alw26I GTCTC 1 cut(s) 1433
AlwI GGATC 4 cut(s) 248, 395, 2189, 2572
AlwNI CAGNNNCTG 1 cut(s) 363
Ama87I CYCGRG 1 cut(s) 1314
AoxI GGCC 7 cut(s) 15, 53, 114, 433, 2195, 2317, 2599
ApeKI GCWGC 4 cut(s) 921, 1094, 1940, 1985
ApoI RAATTY 2 cut(s) 677, 2494
ArsI GACNNNNNNTTYG 2 cut(s) 1045, 1077
AseI ATTAAT 1 cut(s) 1415
Asp700I GAANNNNTTC 3 cut(s) 531, 1161, 1236
AspS9I GGNCC 3 cut(s) 433, 1508, 2195
AsuHPI GGTGA 3 cut(s) 1598, 1700, 2253
AsuII TTCGAA 1 cut(s) 535
AvaI CYCGRG 1 cut(s) 1314
AvaII GGWCC 1 cut(s) 1508
AxyI CCTNAGG 1 cut(s) 2314
BalI TGGCCA 1 cut(s) 116
BanI GGYRCC 1 cut(s) 1792
BarI GAAGNNNNNNTAC 2 cut(s) 1287, 1319
BauI CACGAG 1 cut(s) 807
BbsI GAAGAC 2 cut(s) 1902, 2580
Bbv12I GWGCWC 1 cut(s) 2556
BbvI GCAGC 4 cut(s) 908, 1106, 1927, 1972
BccI CCATC 9 cut(s) 106, 556, 686, 968, 1084, 1174, 1778, 1972, 2186
BciT130I CCWGG 2 cut(s) 950, 1932
BclI TGATCA 3 cut(s) 733, 1513, 2230
BcoDI GTCTC 1 cut(s) 1433
BfaI CTAG 3 cut(s) 1227, 1529, 2091
BfmI CTRYAG 1 cut(s) 1293
BisI GCNGC 4 cut(s) 922, 1095, 1941, 1986
BlsI GCNGC 4 cut(s) 923, 1096, 1942, 1987
Bme1390I CCNGG 2 cut(s) 950, 1932
Bme18I GGWCC 1 cut(s) 1508
BmeT110I CYCGRG 1 cut(s) 1314
BmgT120I GGNCC 3 cut(s) 433, 1508, 2195
BmiI GGNNCC 2 cut(s) 434, 1794
BmrFI CCNGG 2 cut(s) 950, 1932
BmsI GCATC 3 cut(s) 1324, 1762, 2320
BpiI GAAGAC 2 cut(s) 1902, 2580
BplI GAGNNNNNCTC 2 cut(s) 2520, 2552
BpmI CTGGAG 2 cut(s) 1062, 2570
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 3 cut(s) 8, 1974, 2225
BsaAI YACGTR 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 732
BsaJI CCNNGG 4 cut(s) 784, 1699, 1930, 2457
Bsc4I CCNNNNNNNGG 6 cut(s) 337, 503, 1361, 1780, 2167, 2245
Bse118I RCCGGY 1 cut(s) 1861
Bse1I ACTGG 8 cut(s) 124, 178, 849, 1407, 1538, 1545, 1829, 2602
Bse21I CCTNAGG 1 cut(s) 2314
Bse3DI GCAATG 1 cut(s) 2032
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 950, 1932
BseDI CCNNGG 4 cut(s) 784, 1699, 1930, 2457
BseGI GGATG 4 cut(s) 853, 1789, 2197, 2279
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 6 cut(s) 337, 503, 1361, 1780, 2167, 2245
BseMI GCAATG 1 cut(s) 2032
BseMII CTCAG 5 cut(s) 240, 288, 1635, 2070, 2328
BseNI ACTGG 8 cut(s) 124, 178, 849, 1407, 1538, 1545, 1829, 2602
BseRI GAGGAG 3 cut(s) 2228, 2477, 2582
BseXI GCAGC 4 cut(s) 908, 1106, 1927, 1972
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 7 cut(s) 17, 55, 116, 435, 2197, 2319, 2601
BshNI GGYRCC 1 cut(s) 1792
BsiHKAI GWGCWC 1 cut(s) 2556
BsiHKCI CYCGRG 1 cut(s) 1314
BsiSI CCGG 1 cut(s) 1862
BslFI GGGAC 4 cut(s) 110, 479, 1232, 2439
BslI CCNNNNNNNGG 6 cut(s) 337, 503, 1361, 1780, 2167, 2245
BsmAI GTCTC 1 cut(s) 1433
BsmBI CGTCTC 1 cut(s) 1433
BsmFI GGGAC 4 cut(s) 110, 479, 1232, 2439
BsmI GAATGC 2 cut(s) 1254, 1628
BsnI GGCC 7 cut(s) 17, 55, 116, 435, 2197, 2319, 2601
BsoBI CYCGRG 1 cut(s) 1314
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 2556
Bsp1407I TGTACA 2 cut(s) 321, 682
Bsp143I GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
Bsp19I CCATGG 1 cut(s) 784
BspACI CCGC 1 cut(s) 1523
BspANI GGCC 7 cut(s) 17, 55, 116, 435, 2197, 2319, 2601
BspCNI CTCAG 5 cut(s) 241, 289, 1636, 2069, 2327
BspHI TCATGA 1 cut(s) 2299
BspLI GGNNCC 2 cut(s) 434, 1794
BspPI GGATC 4 cut(s) 248, 395, 2189, 2572
BspQI GCTCTTC 1 cut(s) 1063
BspT104I TTCGAA 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 1792
BsrDI GCAATG 1 cut(s) 2032
BsrFI RCCGGY 1 cut(s) 1861
BsrGI TGTACA 2 cut(s) 321, 682
BsrI ACTGG 8 cut(s) 124, 178, 849, 1407, 1538, 1545, 1829, 2602
BssAI RCCGGY 1 cut(s) 1861
BssECI CCNNGG 4 cut(s) 784, 1699, 1930, 2457
BssMI GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
BssSI CACGAG 1 cut(s) 807
BssT1I CCWWGG 3 cut(s) 784, 1699, 2457
Bst2BI CACGAG 1 cut(s) 807
Bst2UI CCWGG 2 cut(s) 950, 1932
Bst4CI ACNGT 9 cut(s) 283, 713, 1104, 1444, 1465, 1656, 1727, 1963, 2579
Bst6I CTCTTC 3 cut(s) 923, 1063, 1426
BstAPI GCANNNNNTGC 1 cut(s) 10
BstAUI TGTACA 2 cut(s) 321, 682
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 4 cut(s) 15, 995, 1252, 1689
BstDEI CTNAG 8 cut(s) 249, 297, 377, 1496, 1644, 2056, 2314, 2513
BstDSI CCRYGG 1 cut(s) 784
BstENI CCTNNNNNAGG 1 cut(s) 2243
BstF5I GGATG 4 cut(s) 853, 1789, 2197, 2279
BstKTI GATC 9 cut(s) 214, 243, 390, 598, 736, 1516, 2184, 2233, 2567
BstMAI GTCTC 1 cut(s) 1433
BstMBI GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
BstMWI GCNNNNNNNGC 4 cut(s) 10, 866, 1357, 2194
BstNI CCWGG 2 cut(s) 950, 1932
BstNSI RCATGY 2 cut(s) 345, 1225
BstSCI CCNGG 2 cut(s) 948, 1930
BstSFI CTRYAG 1 cut(s) 1293
BstV1I GCAGC 4 cut(s) 908, 1106, 1927, 1972
BstV2I GAAGAC 2 cut(s) 1902, 2580
BstX2I RGATCY 3 cut(s) 387, 2181, 2564
BstXI CCANNNNNNTGG 3 cut(s) 956, 1552, 2447
BstYI RGATCY 3 cut(s) 387, 2181, 2564
Bsu36I CCTNAGG 1 cut(s) 2314
BsuRI GGCC 7 cut(s) 17, 55, 116, 435, 2197, 2319, 2601
BtgI CCRYGG 1 cut(s) 784
BtsCI GGATG 4 cut(s) 853, 1789, 2197, 2279
BtsI GCAGTG 2 cut(s) 415, 1355
Cac8I GCNNGC 4 cut(s) 15, 995, 1252, 1689
CaiI CAGNNNCTG 1 cut(s) 363
CciI TCATGA 1 cut(s) 2299
Cfr10I RCCGGY 1 cut(s) 1861
Cfr13I GGNCC 3 cut(s) 433, 1508, 2195
Csp6I GTAC 7 cut(s) 322, 683, 714, 1578, 1832, 2408, 2517
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviQI GTAC 7 cut(s) 322, 683, 714, 1578, 1832, 2408, 2517
DdeI CTNAG 8 cut(s) 249, 297, 377, 1496, 1644, 2056, 2314, 2513
DpnI GATC 9 cut(s) 213, 242, 389, 597, 735, 1515, 2183, 2232, 2566
DpnII GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
DraI TTTAAA 1 cut(s) 2394
EaeI YGGCCR 2 cut(s) 53, 114
Eam1104I CTCTTC 3 cut(s) 923, 1063, 1426
EarI CTCTTC 3 cut(s) 923, 1063, 1426
Eco130I CCWWGG 3 cut(s) 784, 1699, 2457
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 1508
Eco57I CTGAAG 4 cut(s) 773, 2424, 2501, 2589
Eco81I CCTNAGG 1 cut(s) 2314
Eco88I CYCGRG 1 cut(s) 1314
EcoNI CCTNNNNNAGG 1 cut(s) 2243
EcoRI GAATTC 1 cut(s) 2494
EcoRII CCWGG 2 cut(s) 948, 1930
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 784, 1699, 2457
ErhI CCWWGG 3 cut(s) 784, 1699, 2457
Esp3I CGTCTC 1 cut(s) 1433
FalI AAGNNNNNCTT 4 cut(s) 85, 117, 1224, 1256
FaqI GGGAC 4 cut(s) 110, 479, 1232, 2439
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 3 cut(s) 733, 1513, 2230
FblI GTMKAC 1 cut(s) 1613
Fnu4HI GCNGC 4 cut(s) 922, 1095, 1941, 1986
FokI GGATG 4 cut(s) 860, 1796, 2204, 2266
Fsp4HI GCNGC 4 cut(s) 922, 1095, 1941, 1986
FspBI CTAG 3 cut(s) 1227, 1529, 2091
GluI GCNGC 4 cut(s) 922, 1095, 1941, 1986
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 2 cut(s) 1062, 2570
HaeIII GGCC 7 cut(s) 17, 55, 116, 435, 2197, 2319, 2601
HapII CCGG 1 cut(s) 1862
HincII GTYRAC 2 cut(s) 1064, 1857
HindII GTYRAC 2 cut(s) 1064, 1857
HindIII AAGCTT 4 cut(s) 1349, 1685, 1875, 2444
HpaI GTTAAC 1 cut(s) 1857
HpaII CCGG 1 cut(s) 1862
HphI GGTGA 3 cut(s) 1598, 1700, 2253
Hpy166II GTNNAC 5 cut(s) 685, 959, 1064, 1614, 1857
Hpy8I GTNNAC 5 cut(s) 685, 959, 1064, 1614, 1857
HpyCH4III ACNGT 9 cut(s) 283, 713, 1104, 1444, 1465, 1656, 1727, 1963, 2579
HpyCH4IV ACGT 2 cut(s) 475, 543
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 866, 1357, 2194
HpyF3I CTNAG 8 cut(s) 249, 297, 377, 1496, 1644, 2056, 2314, 2513
HpySE526I ACGT 2 cut(s) 475, 543
Ksp22I TGATCA 3 cut(s) 733, 1513, 2230
KspAI GTTAAC 1 cut(s) 1857
Kzo9I GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
LguI GCTCTTC 1 cut(s) 1063
LmnI GCTCC 3 cut(s) 1486, 1948, 2551
Lsp1109I GCAGC 4 cut(s) 908, 1106, 1927, 1972
LweI GCATC 3 cut(s) 1324, 1762, 2320
MaeI CTAG 3 cut(s) 1227, 1529, 2091
MaeII ACGT 2 cut(s) 475, 543
MaeIII GTNAC 7 cut(s) 471, 1145, 1540, 1717, 2259, 2530, 2609
MalI GATC 9 cut(s) 213, 242, 389, 597, 735, 1515, 2183, 2232, 2566
MboI GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
MfeI CAATTG 2 cut(s) 461, 2004
MflI RGATCY 3 cut(s) 387, 2181, 2564
MhlI GDGCHC 1 cut(s) 2556
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 5 cut(s) 92, 196, 631, 947, 1091
MmeI TCCRAC 4 cut(s) 145, 1457, 1573, 2014
Mox20I TGGCCA 1 cut(s) 116
MroXI GAANNNNTTC 3 cut(s) 531, 1161, 1236
MscI TGGCCA 1 cut(s) 116
MslI CAYNNNNRTG 3 cut(s) 690, 2214, 2559
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 1 cut(s) 1862
MspR9I CCNGG 2 cut(s) 950, 1932
MunI CAATTG 2 cut(s) 461, 2004
Mva1269I GAATGC 2 cut(s) 1254, 1628
MvaI CCWGG 2 cut(s) 950, 1932
MwoI GCNNNNNNNGC 4 cut(s) 10, 866, 1357, 2194
NcoI CCATGG 1 cut(s) 784
NdeI CATATG 1 cut(s) 574
NdeII GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
NlaIV GGNNCC 2 cut(s) 434, 1794
NmeAIII GCCGAG 2 cut(s) 317, 2345
NmuCI GTSAC 5 cut(s) 1145, 1540, 1717, 2259, 2609
NspI RCATGY 2 cut(s) 345, 1225
NspV TTCGAA 1 cut(s) 535
PagI TCATGA 1 cut(s) 2299
PciI ACATGT 1 cut(s) 1221
PciSI GCTCTTC 1 cut(s) 1063
PctI GAATGC 2 cut(s) 1254, 1628
PdmI GAANNNNTTC 3 cut(s) 531, 1161, 1236
PfeI GAWTC 7 cut(s) 349, 777, 815, 1327, 2324, 2420, 2545
PflFI GACNNNGTC 1 cut(s) 2579
PflMI CCANNNNNTGG 2 cut(s) 503, 1361
PkrI GCNGC 4 cut(s) 923, 1096, 1942, 1987
PleI GAGTC 5 cut(s) 92, 195, 631, 946, 1090
PpsI GAGTC 5 cut(s) 92, 195, 631, 946, 1090
Ppu21I YACGTR 1 cut(s) 476
PscI ACATGT 1 cut(s) 1221
PshBI ATTAAT 1 cut(s) 1415
PsiI TTATAA 1 cut(s) 833
Psp6I CCWGG 2 cut(s) 948, 1930
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 2 cut(s) 948, 1930
PspN4I GGNNCC 2 cut(s) 434, 1794
PspPI GGNCC 3 cut(s) 433, 1508, 2195
PstNI CAGNNNCTG 1 cut(s) 363
PsuI RGATCY 3 cut(s) 387, 2181, 2564
PsyI GACNNNGTC 1 cut(s) 2579
RsaI GTAC 7 cut(s) 323, 684, 715, 1579, 1833, 2409, 2518
RsaNI GTAC 7 cut(s) 322, 683, 714, 1578, 1832, 2408, 2517
RseI CAYNNNNRTG 3 cut(s) 690, 2214, 2559
SapI GCTCTTC 1 cut(s) 1063
SatI GCNGC 4 cut(s) 922, 1095, 1941, 1986
Sau3AI GATC 9 cut(s) 211, 240, 387, 595, 733, 1513, 2181, 2230, 2564
Sau96I GGNCC 3 cut(s) 433, 1508, 2195
SchI GAGTC 5 cut(s) 92, 196, 631, 947, 1091
ScrFI CCNGG 2 cut(s) 950, 1932
SduI GDGCHC 1 cut(s) 2556
SfaNI GCATC 3 cut(s) 1324, 1762, 2320
SfcI CTRYAG 1 cut(s) 1293
SfuI TTCGAA 1 cut(s) 535
SinI GGWCC 1 cut(s) 1508
SmiMI CAYNNNNRTG 3 cut(s) 690, 2214, 2559
SmlI CTYRAG 3 cut(s) 23, 1989, 2240
SmoI CTYRAG 3 cut(s) 23, 1989, 2240
SsiI CCGC 1 cut(s) 1523
SspI AATATT 1 cut(s) 32
SspMI CTAG 3 cut(s) 1227, 1529, 2091
StyD4I CCNGG 2 cut(s) 948, 1930
StyI CCWWGG 3 cut(s) 784, 1699, 2457
TaaI ACNGT 9 cut(s) 283, 713, 1104, 1444, 1465, 1656, 1727, 1963, 2579
TaiI ACGT 2 cut(s) 478, 546
TaqI TCGA 2 cut(s) 535, 2586
TatI WGTACW 4 cut(s) 321, 682, 1577, 2407
TfiI GAWTC 7 cut(s) 349, 777, 815, 1327, 2324, 2420, 2545
TseFI GTSAC 5 cut(s) 1145, 1540, 1717, 2259, 2609
TseI GCWGC 4 cut(s) 921, 1094, 1940, 1985
Tsp45I GTSAC 5 cut(s) 1145, 1540, 1717, 2259, 2609
TspDTI ATGAA 7 cut(s) 36, 423, 855, 1767, 1908, 2264, 2316
TspGWI ACGGA 1 cut(s) 1451
Tth111I GACNNNGTC 1 cut(s) 2579
Van91I CCANNNNNTGG 2 cut(s) 503, 1361
VpaK11BI GGWCC 1 cut(s) 1508
VspI ATTAAT 1 cut(s) 1415
XagI CCTNNNNNAGG 1 cut(s) 2243
XapI RAATTY 2 cut(s) 677, 2494
XbaI TCTAGA 2 cut(s) 1226, 2090
XceI RCATGY 2 cut(s) 345, 1225
XmiI GTMKAC 1 cut(s) 1613
XmnI GAANNNNTTC 3 cut(s) 531, 1161, 1236
XspI CTAG 3 cut(s) 1227, 1529, 2091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.