Rorug05G0428700

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003692
Physical Location & Seq
Reverse (-)
59607027 .. 59609945
2919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0428700.1

Sequence Viewer

Length: 1710 bp
ATGGAAAACAGAGTCATTAGCCTCCTGGTCATTGCGCTCATTATGTGCTATGGGTTGTCAGCGGCCGTTGGATTTTATCGAGATGAAGACTGGGGGAAGCAGAGAGAAGACCCCGATCAAGAGCAGGAAGAGCAAGAGCAAGAAGCGATGATGGGAGACGAGGATTGGTTCTTGTTGCAGAACTCAAAGCAGGTGGTGAAAACTCGAGCTGGTGAAATGAGAGTGGTGAAAAGCGTAGGTGGGAGGATTGTTGATAAGCCAATGCATATTGGGTTTATTACAATGGAGCCCAAGTCCCTCTTTGTACCTCAGTACATGGACTCCAGCTTGATCCTCTTCGTCCATTCTGGGGAAGCAAAGGTAGGGCTGATATACAGAGATGAACTGGGAGAGAGGAGATTGAGGGCTGGGGATGTGTACAGAATTCCAGCTGGTTCGCCGTTTTATTTGGTGAATACAGGGGAGGGTCAGAGACTTCACATCATCTGTAGCGTTGATACATCTGAGAGTTTGGGAATGGGTACTTTGCAGTCTTTCTACATTGGTGGAGGATATAACCCAGCATCTGTACTTGCTGGATTTGACCAGGAAATACTCGAAAGTGCATTCAATGTTACATCATCAGAACTAAGTGATTTCTTGTCAAGGCAACAAGATGGTCCGATTGTATATGTGACTGATTCACCTCATCCACCAAGCCTTTGGTCAAGATTCCTAGAGCTGAAAGAGCATGACAGACTCCAAAATATGAAGAAAATGGTGGGCTTCACAAAAGAACCTGATCACCAAGAAGAAGACCAATCATTAGCATGGTCATGGAGGAAGCTTCTCAACTCTGTGTTCGGATCAAATGACAAGAGAAGAGACGATCCCGATACTTACAAGGGAAAAGGAAAATCTCATGATGCTTACAACCTCTTCAAGAGATCTGCAGATTTCAAAAACGACTATGGCTGGAGCAAGGCACTTGACGAATCTGATTACTCGCCACTAAAAGACTCTGGCGTTGGTGTCTTCCTTGTCAACCTTTCTGCAGGGTCAATGATGGCACCACATGTGAACCCAATAGCAACAGAGTATGGGATTGTATTGAGAGGCTCTGGCACAATACAGATTGTATTTCCAAACGGAACCTCGGCAATGAATACTAAAGTTCAAGAAGGAGATGTGTTTTGGGTCCCAAGGTACTTCCCATTCTGTCAAATTGCATCAAGAACTGGTCCTCTTGAGTTCTTTGGATTCACCACCTCGGCACGCAAGAACAGGCCACAGTTCTTGGTTGGTGCTAGCTCTGTTCTTAGGGAGCTCAGAGGACCTGAGCTTGCAGCTGCTTTTGGTGTGAATGAGGATCGGTTGAGTCGCATTGTTCATGCTCAGCGTGAGTCTGTGATCTTGCCTTCGGTGCAAGCAGCTCCTCCTTACAAGGGGGAGGAGAGAGAGCCAACCCCGGAGGAGGAGACAGGGAAGCAAACCCCATACAAGGAGGACGAGAGGAGGCAGGAAGACACGAGGAGGCGGGAAGACAAGAGGGAGCAATCAGCTGAGGAAGATGAGAGAAGGCAACCCCCATACAAGGAAGATGAGAGGCAGCAGTCGCAACAGGAGAAGCAGAGAAGGCAACCTCCATACGGGGAAGGGGATGTGATGTTTCAGAGGCTCCCAAAGGTGATCAAGAGTTTCGGGAATGAGATGGTTATGGGTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

569

Amino Acids

64.65

Weight (kDa)

5.29

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 91 - 209 4e-09 Cupin
Cupin_1 PF00190 304 - 449 1.5e-32 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 181
Acc36I ACCTGC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 1048
AciI CCGC 2 cut(s) 62, 1516
AclWI GGATC 4 cut(s) 325, 853, 863, 1356
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 423
AfaI GTAC 6 cut(s) 306, 314, 419, 523, 570, 1187
AfiI CCNNNNNNNGG 6 cut(s) 349, 1424, 1453, 1480, 1573, 1628
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 4 cut(s) 610, 922, 940, 1157
AjnI CCWGG 2 cut(s) 24, 585
Alw21I GWGCWC 1 cut(s) 1308
Alw26I GTCTC 4 cut(s) 150, 466, 858, 1451
AlwI GGATC 4 cut(s) 325, 853, 863, 1356
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 2 cut(s) 63, 1265
ApeKI GCWGC 4 cut(s) 1325, 1328, 1409, 1588
ApoI RAATTY 1 cut(s) 423
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 4 cut(s) 659, 1177, 1220, 1313
AsuC2I CCSGG 1 cut(s) 1448
AsuHPI GGTGA 8 cut(s) 208, 224, 238, 463, 675, 776, 1234, 1678
AsuNHI GCTAGC 1 cut(s) 1286
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 4 cut(s) 659, 1177, 1220, 1313
BanI GGYRCC 1 cut(s) 1048
BanII GRGCYC 2 cut(s) 291, 1308
BauI CACGAG 1 cut(s) 1507
BbsI GAAGAC 6 cut(s) 93, 114, 801, 1006, 1509, 1527
Bbv12I GWGCWC 1 cut(s) 1308
BbvCI CCTCAGC 1 cut(s) 1542
BbvI GCAGC 4 cut(s) 1315, 1337, 1421, 1600
BccI CCATC 4 cut(s) 145, 650, 1039, 1684
BceAI ACGGC 2 cut(s) 50, 424
BciT130I CCWGG 2 cut(s) 26, 587
BclI TGATCA 2 cut(s) 781, 1668
BcnI CCSGG 1 cut(s) 1448
BcoDI GTCTC 4 cut(s) 150, 466, 858, 1451
BfaI CTAG 2 cut(s) 716, 1287
BfmI CTRYAG 3 cut(s) 487, 930, 1032
BfuAI ACCTGC 1 cut(s) 181
BglII AGATCT 1 cut(s) 926
BisI GCNGC 5 cut(s) 63, 1326, 1329, 1410, 1589
BlpI GCTNAGC 1 cut(s) 1374
BlsI GCNGC 5 cut(s) 64, 1327, 1330, 1411, 1590
Bme1390I CCNGG 3 cut(s) 26, 587, 1448
Bme18I GGWCC 4 cut(s) 659, 1177, 1220, 1313
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 4 cut(s) 659, 1177, 1220, 1313
BmiI GGNNCC 6 cut(s) 288, 1050, 1132, 1178, 1179, 1658
BmrFI CCNGG 3 cut(s) 26, 587, 1448
BmrI ACTGGG 2 cut(s) 100, 395
BmsI GCATC 3 cut(s) 572, 895, 1217
BmtI GCTAGC 1 cut(s) 1290
BmuI ACTGGG 2 cut(s) 100, 395
BpiI GAAGAC 6 cut(s) 93, 114, 801, 1006, 1509, 1527
BpmI CTGGAG 2 cut(s) 307, 976
Bpu10I CCTNAGC 2 cut(s) 1317, 1542
Bpu1102I GCTNAGC 1 cut(s) 1374
BpuEI CTTGAG 1 cut(s) 1247
BpuMI CCSGG 1 cut(s) 1448
BsaJI CCNNGG 4 cut(s) 1134, 1181, 1248, 1446
BsaXI ACNNNNNCTCC 4 cut(s) 278, 305, 308, 335
Bsc4I CCNNNNNNNGG 6 cut(s) 349, 1424, 1453, 1480, 1573, 1628
Bse1I ACTGG 3 cut(s) 95, 390, 1222
Bse3DI GCAATG 2 cut(s) 30, 1146
BseBI CCWGG 2 cut(s) 26, 587
BseDI CCNNGG 4 cut(s) 1134, 1181, 1248, 1446
BseGI GGATG 3 cut(s) 418, 688, 1645
BseLI CCNNNNNNNGG 6 cut(s) 349, 1424, 1453, 1480, 1573, 1628
BseMI GCAATG 2 cut(s) 30, 1146
BseMII CTCAG 6 cut(s) 323, 495, 1308, 1321, 1388, 1533
BseNI ACTGG 3 cut(s) 95, 390, 1222
BseRI GAGGAG 7 cut(s) 409, 1404, 1445, 1466, 1469, 1507, 1525
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 1315, 1337, 1421, 1600
BseYI CCCAGC 2 cut(s) 407, 559
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 2 cut(s) 65, 1267
BshNI GGYRCC 1 cut(s) 1048
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 1308
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 1 cut(s) 1448
BslFI GGGAC 2 cut(s) 280, 1163
BslI CCNNNNNNNGG 6 cut(s) 349, 1424, 1453, 1480, 1573, 1628
BsmAI GTCTC 4 cut(s) 150, 466, 858, 1451
BsmBI CGTCTC 2 cut(s) 150, 858
BsmFI GGGAC 2 cut(s) 280, 1163
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 2 cut(s) 65, 1267
BsoBI CYCGRG 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 291, 1308
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
Bsp1720I GCTNAGC 1 cut(s) 1374
BspACI CCGC 2 cut(s) 62, 1516
BspANI GGCC 2 cut(s) 65, 1267
BspCNI CTCAG 6 cut(s) 322, 496, 1309, 1320, 1387, 1534
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 6 cut(s) 288, 1050, 1132, 1178, 1179, 1658
BspMAI CTGCAG 2 cut(s) 934, 1036
BspMI ACCTGC 1 cut(s) 181
BspOI GCTAGC 1 cut(s) 1290
BspPI GGATC 4 cut(s) 325, 853, 863, 1356
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 1048
BsrDI GCAATG 2 cut(s) 30, 1146
BsrGI TGTACA 1 cut(s) 417
BsrI ACTGG 3 cut(s) 95, 390, 1222
BssECI CCNNGG 4 cut(s) 1134, 1181, 1248, 1446
BssMI GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
BssSI CACGAG 1 cut(s) 1507
BssT1I CCWWGG 1 cut(s) 1181
Bst2BI CACGAG 1 cut(s) 1507
Bst2UI CCWGG 2 cut(s) 26, 587
Bst4CI ACNGT 1 cut(s) 1272
Bst6I CTCTTC 4 cut(s) 123, 341, 856, 923
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 4 cut(s) 1255, 1288, 1323, 1407
BstDEI CTNAG 8 cut(s) 309, 504, 629, 1298, 1307, 1317, 1374, 1542
BstF5I GGATG 3 cut(s) 418, 688, 1645
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 9 cut(s) 118, 333, 784, 848, 871, 929, 1351, 1392, 1671
BstMAI GTCTC 4 cut(s) 150, 466, 858, 1451
BstMBI GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 5 cut(s) 130, 727, 1402, 1594, 1615
BstNI CCWGG 2 cut(s) 26, 587
BstNSI RCATGY 1 cut(s) 1058
BstSCI CCNGG 3 cut(s) 24, 585, 1446
BstSFI CTRYAG 3 cut(s) 487, 930, 1032
BstV1I GCAGC 4 cut(s) 1315, 1337, 1421, 1600
BstV2I GAAGAC 6 cut(s) 93, 114, 801, 1006, 1509, 1527
BstX2I RGATCY 1 cut(s) 926
BstXI CCANNNNNNTGG 1 cut(s) 702
BstYI RGATCY 1 cut(s) 926
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 2 cut(s) 65, 1267
BtgZI GCGATG 1 cut(s) 161
BtsCI GGATG 3 cut(s) 418, 688, 1645
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 4 cut(s) 1255, 1288, 1323, 1407
CaiI CAGNNNCTG 1 cut(s) 566
CciI TCATGA 1 cut(s) 901
CfoI GCGC 1 cut(s) 37
Cfr13I GGNCC 4 cut(s) 659, 1177, 1220, 1313
Csp6I GTAC 6 cut(s) 305, 313, 418, 522, 569, 1186
CspCI CAANNNNNGTGG 4 cut(s) 174, 209, 1257, 1292
CviAII CATG 7 cut(s) 316, 731, 810, 816, 902, 1055, 1370
CviQI GTAC 6 cut(s) 305, 313, 418, 522, 569, 1186
DdeI CTNAG 8 cut(s) 309, 504, 629, 1298, 1307, 1317, 1374, 1542
DpnI GATC 9 cut(s) 117, 332, 783, 847, 870, 928, 1350, 1391, 1670
DpnII GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1104I CTCTTC 4 cut(s) 123, 341, 856, 923
EarI CTCTTC 4 cut(s) 123, 341, 856, 923
Ecl136II GAGCTC 1 cut(s) 1306
EclXI CGGCCG 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 1181
Eco24I GRGCYC 2 cut(s) 291, 1308
Eco47I GGWCC 4 cut(s) 659, 1177, 1220, 1313
Eco52I CGGCCG 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 1306
Eco88I CYCGRG 1 cut(s) 204
EcoICRI GAGCTC 1 cut(s) 1306
EcoO109I RGGNCCY 2 cut(s) 1177, 1313
EcoRI GAATTC 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 24, 585
EcoT14I CCWWGG 1 cut(s) 1181
EcoT22I ATGCAT 1 cut(s) 267
EcoT38I GRGCYC 2 cut(s) 291, 1308
ErhI CCWWGG 1 cut(s) 1181
Esp3I CGTCTC 2 cut(s) 150, 858
FaeI CATG 7 cut(s) 319, 734, 813, 819, 905, 1058, 1373
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 2 cut(s) 280, 1163
FatI CATG 7 cut(s) 315, 730, 809, 815, 901, 1054, 1369
FauI CCCGC 1 cut(s) 1509
FbaI TGATCA 2 cut(s) 781, 1668
Fnu4HI GCNGC 5 cut(s) 63, 1326, 1329, 1410, 1589
FokI GGATG 3 cut(s) 425, 675, 1652
FriOI GRGCYC 2 cut(s) 291, 1308
Fsp4HI GCNGC 5 cut(s) 63, 1326, 1329, 1410, 1589
FspBI CTAG 2 cut(s) 716, 1287
GlaI GCGC 1 cut(s) 36
GluI GCNGC 5 cut(s) 63, 1326, 1329, 1410, 1589
GsaI CCCAGC 2 cut(s) 411, 563
GsuI CTGGAG 2 cut(s) 307, 976
HaeIII GGCC 2 cut(s) 65, 1267
HapII CCGG 1 cut(s) 1448
HhaI GCGC 1 cut(s) 37
Hin1II CATG 7 cut(s) 319, 734, 813, 819, 905, 1058, 1373
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 1 cut(s) 824
HpaII CCGG 1 cut(s) 1448
HphI GGTGA 8 cut(s) 208, 224, 238, 463, 675, 776, 1234, 1678
Hpy166II GTNNAC 3 cut(s) 418, 1024, 1060
Hpy188I TCNGA 8 cut(s) 471, 505, 625, 663, 845, 979, 1310, 1653
Hpy8I GTNNAC 3 cut(s) 418, 1024, 1060
HpyAV CCTTC 5 cut(s) 1154, 1407, 1551, 1608, 1628
HpyCH4III ACNGT 1 cut(s) 1272
HpyCH4V TGCA 9 cut(s) 178, 265, 529, 605, 932, 1034, 1208, 1325, 1405
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 727, 1402, 1594, 1615
HpyF3I CTNAG 8 cut(s) 309, 504, 629, 1298, 1307, 1317, 1374, 1542
Hsp92II CATG 7 cut(s) 319, 734, 813, 819, 905, 1058, 1373
HspAI GCGC 1 cut(s) 35
KflI GGGWCCC 1 cut(s) 1177
Ksp22I TGATCA 2 cut(s) 781, 1668
Kzo9I GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 6 cut(s) 286, 957, 1303, 1417, 1531, 1662
Lsp1109I GCAGC 4 cut(s) 1315, 1337, 1421, 1600
LweI GCATC 3 cut(s) 572, 895, 1217
MaeI CTAG 2 cut(s) 716, 1287
MaeIII GTNAC 2 cut(s) 613, 673
MalI GATC 9 cut(s) 117, 332, 783, 847, 870, 928, 1350, 1391, 1670
MboI GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 2 cut(s) 291, 1308
MluCI AATT 2 cut(s) 423, 1203
MlyI GAGTC 6 cut(s) 21, 314, 732, 992, 1366, 1391
MmeI TCCRAC 1 cut(s) 49
Mph1103I ATGCAT 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 808, 814
MspA1I CMGCKG 4 cut(s) 62, 431, 1328, 1541
MspI CCGG 1 cut(s) 1448
MspR9I CCNGG 3 cut(s) 26, 587, 1448
Mva1269I GAATGC 1 cut(s) 605
MvaI CCWGG 2 cut(s) 26, 587
MwoI GCNNNNNNNGC 5 cut(s) 130, 727, 1402, 1594, 1615
NciI CCSGG 1 cut(s) 1448
NdeII GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
NheI GCTAGC 1 cut(s) 1286
NlaIII CATG 7 cut(s) 319, 734, 813, 819, 905, 1058, 1373
NlaIV GGNNCC 6 cut(s) 288, 1050, 1132, 1178, 1179, 1658
NmeAIII GCCGAG 2 cut(s) 1115, 1229
NmuCI GTSAC 1 cut(s) 673
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 1058
PaeR7I CTCGAG 1 cut(s) 204
PagI TCATGA 1 cut(s) 901
PaqCI CACCTGC 1 cut(s) 181
PciI ACATGT 1 cut(s) 1054
PciSI GCTCTTC 1 cut(s) 123
PctI GAATGC 1 cut(s) 605
PfeI GAWTC 4 cut(s) 680, 711, 974, 1239
PkrI GCNGC 5 cut(s) 64, 1327, 1330, 1411, 1590
PleI GAGTC 6 cut(s) 20, 314, 732, 992, 1365, 1390
PpsI GAGTC 6 cut(s) 20, 314, 732, 992, 1365, 1390
PpuMI RGGWCCY 2 cut(s) 1177, 1313
PscI ACATGT 1 cut(s) 1054
Psp124BI GAGCTC 1 cut(s) 1308
Psp5II RGGWCCY 2 cut(s) 1177, 1313
Psp6I CCWGG 2 cut(s) 24, 585
PspFI CCCAGC 2 cut(s) 407, 559
PspGI CCWGG 2 cut(s) 24, 585
PspN4I GGNNCC 6 cut(s) 288, 1050, 1132, 1178, 1179, 1658
PspPI GGNCC 4 cut(s) 659, 1177, 1220, 1313
PspPPI RGGWCCY 2 cut(s) 1177, 1313
PspXI VCTCGAGB 1 cut(s) 204
PstI CTGCAG 2 cut(s) 934, 1036
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 926
PvuII CAGCTG 3 cut(s) 431, 1328, 1541
RsaI GTAC 6 cut(s) 306, 314, 419, 523, 570, 1187
RsaNI GTAC 6 cut(s) 305, 313, 418, 522, 569, 1186
RseI CAYNNNNRTG 2 cut(s) 808, 814
SacI GAGCTC 1 cut(s) 1308
SapI GCTCTTC 1 cut(s) 123
SatI GCNGC 5 cut(s) 63, 1326, 1329, 1410, 1589
Sau3AI GATC 9 cut(s) 115, 330, 781, 845, 868, 926, 1348, 1389, 1668
Sau96I GGNCC 4 cut(s) 659, 1177, 1220, 1313
SchI GAGTC 6 cut(s) 21, 314, 732, 992, 1366, 1391
ScrFI CCNGG 3 cut(s) 26, 587, 1448
SduI GDGCHC 2 cut(s) 291, 1308
SfaNI GCATC 3 cut(s) 572, 895, 1217
SfcI CTRYAG 3 cut(s) 487, 930, 1032
Sfr274I CTCGAG 1 cut(s) 204
SinI GGWCC 4 cut(s) 659, 1177, 1220, 1313
SlaI CTCGAG 1 cut(s) 204
SmiMI CAYNNNNRTG 2 cut(s) 808, 814
SmlI CTYRAG 2 cut(s) 204, 1226
SmoI CTYRAG 2 cut(s) 204, 1226
Sse9I AATT 2 cut(s) 423, 1203
SsiI CCGC 2 cut(s) 62, 1516
SspMI CTAG 2 cut(s) 716, 1287
SstI GAGCTC 1 cut(s) 1308
StyD4I CCNGG 3 cut(s) 24, 585, 1446
StyI CCWWGG 1 cut(s) 1181
TaaI ACNGT 1 cut(s) 1272
TaqI TCGA 3 cut(s) 79, 205, 597
TasI AATT 2 cut(s) 423, 1203
TatI WGTACW 3 cut(s) 312, 417, 568
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 4 cut(s) 680, 711, 974, 1239
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 4 cut(s) 1325, 1328, 1409, 1588
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 5 cut(s) 99, 396, 764, 1157, 1358
TspGWI ACGGA 1 cut(s) 1143
VpaK11BI GGWCC 4 cut(s) 659, 1177, 1220, 1313
XapI RAATTY 1 cut(s) 423
XceI RCATGY 1 cut(s) 1058
XcmI CCANNNNNNNNNTGG 1 cut(s) 699
XhoI CTCGAG 1 cut(s) 204
XspI CTAG 2 cut(s) 716, 1287
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.