RLG00000036442

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003692
Physical Location & Seq
Forward (+)
80685203 .. 80689903
4701 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036442

Sequence Viewer

Length: 1377 bp
ATGTCGTCTATGCAGGCCATTCTCAAGAAATATGGGTTTCCTTCATTTCGGCCATACCAAAAGAAAGTGATTGAGAAAATCATAGCAGGAAGGGACTCTTTGATTGTCATGGCCACTGGAAGTGGCAAGTCTTTGTGCTATCAAGTGCCTCCTTTGGTTGTTGGAAAGACTGGTGTGGTTGTGAGCCCTCTTTTATCCTTGATGCAAGATCAGGTAATGGGTTTGAAACAAAGAGGGATCAGAGCTGAGTATATGGGTAGTAGTCAAACTGATAGCACTGTCCAGAGCCGAGCCGAGAGTGGTCAATTTGATATCTTGTACATGACCCCTGAAAAGGCATGTAAGATTCCTGACAGCTTCTGGTCAAAATTACGAAGGACTGGAATCTGCCTGTTTGCTGTTGATGAAGCACACTGCATATCGGAGTGGGGCCATGATTTCAGGGTTCAAATGGACATTGTTAATTCCTTGAAGATGAAAAACCCATATTACAGAATAGGCTTCTTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTCATGAACTAGCTCAAGAAGTTTCCAAATTTGTATTCATTTCACTGAAGGAGGTGGGCATTAAGGCTGGAATCTACCATAGTGAGATGGATAATAAAGCTCGTGCCGAATCCCATAGATTATTTATAAGAGATGAAGTAGATGTCATGGTTGCTACTATTGCTTTCGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCATTATGGCTGCCCGAAGAATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACCTCATAAAAGCTGAATTTTATGCTGGAGAGTGTCAAACAGAGGAAGGGCCTAGTTGGCTCTTTCCTAGTGGCCAAGTAGTTGAAAGTCGACGAAGAGCTGTTATAAAATCTTTGAGGGCAGTACAACAATATTGCTTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTTACTTTGTGGAAATCTTTCCATCTGATAAATGTGGTAATTGTGATAATTGTATTTCCCCAAAGAAGGAGATGGACATGTCTAGAGAAGCCTATCTTCTAATGGCTTGCATTCAATCATGTCGGGGTACATGCGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTGTCGGGCCAAAAAGATTCTTGCTGCCGAGTATGACAAGCTTCCACTGCATGGACTTGGGAAAGACTATTCTGCAAATTGGTGGAAAACACTTGCTTACCAATTAATCTCTTATGGTATAGTAGGGAAGCATTTTGAATATGTGGAAGTAGGAGATTGGGAGATTGATGGTAACTGGTGTGTTCCCGCTGTGTATTTTAGCAATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

459

Amino Acids

51.97

Weight (kDa)

8.87

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 15 - 143 6.7e-06 Type III restriction enzyme, res subunit
DEAD PF00270 17 - 158 1e-21 DEAD/DEAH box helicase
Helicase_C PF00271 197 - 263 1.7e-10 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 303 - 351 3.4e-14 RecQ zinc-binding
RQC PF09382 354 - 435 1e-17 RQC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 689, 935
AccB7I CCANNNNNTGG 1 cut(s) 1217
AccI GTMKAC 1 cut(s) 919
AciI CCGC 2 cut(s) 1134, 1353
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 3 cut(s) 50, 111, 901
AcsI RAATTY 2 cut(s) 590, 845
AcuI CTGAAG 1 cut(s) 629
AfaI GTAC 3 cut(s) 320, 954, 1129
AfiI CCNNNNNNNGG 4 cut(s) 334, 788, 1066, 1217
AflIII ACRYGT 1 cut(s) 1077
AgsI TTSAA 7 cut(s) 226, 449, 472, 544, 914, 1115, 1304
AjuI GAANNNNNNNTTGG 2 cut(s) 581, 613
AluBI AGCT 7 cut(s) 245, 357, 575, 662, 842, 929, 1207
AluI AGCT 7 cut(s) 245, 357, 575, 662, 842, 929, 1207
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 360
AoxI GGCC 7 cut(s) 15, 50, 111, 430, 878, 901, 1173
ApeKI GCWGC 2 cut(s) 777, 1190
ApoI RAATTY 2 cut(s) 590, 845
AseI ATTAAT 1 cut(s) 1271
Asp700I GAANNNNTTC 1 cut(s) 1017
AspS9I GGNCC 3 cut(s) 430, 878, 1173
BalI TGGCCA 2 cut(s) 113, 903
BanII GRGCYC 1 cut(s) 188
BarI GAAGNNNNNNTAC 2 cut(s) 1143, 1175
BauI CACGAG 1 cut(s) 663
BbvI GCAGC 2 cut(s) 764, 1177
BccI CCATC 4 cut(s) 643, 1030, 1066, 1328
BfaI CTAG 5 cut(s) 572, 882, 897, 1083, 1375
BfmI CTRYAG 1 cut(s) 1149
BglI GCCNNNNNGGC 1 cut(s) 886
BisI GCNGC 2 cut(s) 778, 1191
BlsI GCNGC 2 cut(s) 779, 1192
BmgT120I GGNCC 3 cut(s) 430, 878, 1173
BmiI GGNNCC 1 cut(s) 431
BmsI GCATC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 876
BpuEI CTTGAG 2 cut(s) 8, 561
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 788, 1066, 1217
Bse1I ACTGG 4 cut(s) 121, 175, 385, 1346
BseLI CCNNNNNNNGG 4 cut(s) 334, 788, 1066, 1217
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 4 cut(s) 121, 175, 385, 1346
BseXI GCAGC 2 cut(s) 764, 1177
BshFI GGCC 7 cut(s) 17, 52, 113, 432, 880, 903, 1175
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 4 cut(s) 334, 788, 1066, 1217
BsmFI GGGAC 1 cut(s) 107
BsmI GAATGC 1 cut(s) 1110
BsnI GGCC 7 cut(s) 17, 52, 113, 432, 880, 903, 1175
Bsp1286I GDGCHC 1 cut(s) 188
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 3 cut(s) 208, 237, 508
BspACI CCGC 2 cut(s) 1134, 1353
BspANI GGCC 7 cut(s) 17, 52, 113, 432, 880, 903, 1175
BspCNI CTCAG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 431
BspPI GGATC 1 cut(s) 245
BspQI GCTCTTC 1 cut(s) 919
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 4 cut(s) 121, 175, 385, 1346
BssMI GATC 3 cut(s) 208, 237, 508
BssSI CACGAG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 663
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 919
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 3 cut(s) 15, 809, 1108
BstDEI CTNAG 1 cut(s) 246
BstKTI GATC 3 cut(s) 211, 240, 511
BstMBI GATC 3 cut(s) 208, 237, 508
BstMWI GCNNNNNNNGC 3 cut(s) 722, 886, 1213
BstNSI RCATGY 3 cut(s) 342, 1081, 1134
BstSFI CTRYAG 1 cut(s) 1149
BstV1I GCAGC 2 cut(s) 764, 1177
BsuRI GGCC 7 cut(s) 17, 52, 113, 432, 880, 903, 1175
BtsI GCAGTG 2 cut(s) 412, 1211
BtsIMutI CAGTG 5 cut(s) 114, 276, 412, 605, 1211
Cac8I GCNNGC 3 cut(s) 15, 809, 1108
CaiI CAGNNNCTG 1 cut(s) 360
CciI TCATGA 1 cut(s) 565
Cfr13I GGNCC 3 cut(s) 430, 878, 1173
Csp6I GTAC 3 cut(s) 319, 953, 1128
CviQI GTAC 3 cut(s) 319, 953, 1128
DdeI CTNAG 1 cut(s) 246
DpnI GATC 3 cut(s) 210, 239, 510
DpnII GATC 3 cut(s) 208, 237, 508
EaeI YGGCCR 3 cut(s) 50, 111, 901
Eam1104I CTCTTC 1 cut(s) 919
EarI CTCTTC 1 cut(s) 919
Eco24I GRGCYC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 878
EcoRV GATATC 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 188
FalI AAGNNNNNCTT 4 cut(s) 82, 114, 1080, 1112
FaqI GGGAC 1 cut(s) 107
FauI CCCGC 1 cut(s) 1360
FblI GTMKAC 1 cut(s) 919
Fnu4HI GCNGC 2 cut(s) 778, 1191
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 2 cut(s) 778, 1191
FspBI CTAG 5 cut(s) 572, 882, 897, 1083, 1375
GluI GCNGC 2 cut(s) 778, 1191
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 7 cut(s) 17, 52, 113, 432, 880, 903, 1175
HincII GTYRAC 1 cut(s) 920
HindII GTYRAC 1 cut(s) 920
HindIII AAGCTT 1 cut(s) 1205
HinfI GANTC 7 cut(s) 95, 346, 384, 550, 633, 671, 1183
Hpy166II GTNNAC 1 cut(s) 920
Hpy188I TCNGA 5 cut(s) 242, 424, 549, 1027, 1140
Hpy188III TCNNGA 6 cut(s) 25, 283, 350, 566, 578, 1083
Hpy8I GTNNAC 1 cut(s) 920
Hpy99I CGWCG 1 cut(s) 924
HpyAV CCTTC 7 cut(s) 51, 84, 369, 604, 869, 978, 1060
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 7 cut(s) 13, 205, 417, 981, 1110, 1216, 1241
HpyF10VI GCNNNNNNNGC 3 cut(s) 722, 886, 1213
HpyF3I CTNAG 1 cut(s) 246
Kzo9I GATC 3 cut(s) 208, 237, 508
LguI GCTCTTC 1 cut(s) 919
Lsp1109I GCAGC 2 cut(s) 764, 1177
LweI GCATC 1 cut(s) 192
MaeI CTAG 5 cut(s) 572, 882, 897, 1083, 1375
MaeIII GTNAC 2 cut(s) 1001, 1337
MalI GATC 3 cut(s) 210, 239, 510
MboI GATC 3 cut(s) 208, 237, 508
MboII GAAGA 5 cut(s) 484, 796, 936, 1088, 1151
MhlI GDGCHC 1 cut(s) 188
MlsI TGGCCA 2 cut(s) 113, 903
MluCI AATT 9 cut(s) 305, 368, 463, 590, 845, 1039, 1048, 1243, 1268
MluNI TGGCCA 2 cut(s) 113, 903
MlyI GAGTC 2 cut(s) 89, 544
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 7 cut(s) 159, 198, 227, 607, 842, 865, 939
Mox20I TGGCCA 2 cut(s) 113, 903
MroXI GAANNNNTTC 1 cut(s) 1017
MscI TGGCCA 2 cut(s) 113, 903
MseI TTAA 3 cut(s) 462, 624, 1271
Msp20I TGGCCA 2 cut(s) 113, 903
MspA1I CMGCKG 1 cut(s) 1355
Mva1269I GAATGC 1 cut(s) 1110
MwoI GCNNNNNNNGC 3 cut(s) 722, 886, 1213
NdeII GATC 3 cut(s) 208, 237, 508
NlaIV GGNNCC 1 cut(s) 431
NmeAIII GCCGAG 3 cut(s) 314, 319, 1219
NspI RCATGY 3 cut(s) 342, 1081, 1134
PagI TCATGA 1 cut(s) 565
PciI ACATGT 1 cut(s) 1077
PciSI GCTCTTC 1 cut(s) 919
PctI GAATGC 1 cut(s) 1110
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 5 cut(s) 346, 384, 633, 671, 1183
PflMI CCANNNNNTGG 1 cut(s) 1217
PkrI GCNGC 2 cut(s) 779, 1192
PleI GAGTC 2 cut(s) 89, 544
PpsI GAGTC 2 cut(s) 89, 544
PscI ACATGT 1 cut(s) 1077
PshBI ATTAAT 1 cut(s) 1271
PsiI TTATAA 2 cut(s) 689, 935
PspN4I GGNNCC 1 cut(s) 431
PspPI GGNCC 3 cut(s) 430, 878, 1173
PstNI CAGNNNCTG 1 cut(s) 360
RsaI GTAC 3 cut(s) 320, 954, 1129
RsaNI GTAC 3 cut(s) 319, 953, 1128
SalI GTCGAC 1 cut(s) 918
SapI GCTCTTC 1 cut(s) 919
SaqAI TTAA 3 cut(s) 462, 624, 1271
SatI GCNGC 2 cut(s) 778, 1191
Sau3AI GATC 3 cut(s) 208, 237, 508
Sau96I GGNCC 3 cut(s) 430, 878, 1173
SchI GAGTC 2 cut(s) 89, 544
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 192
SfcI CTRYAG 1 cut(s) 1149
SmlI CTYRAG 2 cut(s) 23, 576
SmoI CTYRAG 2 cut(s) 23, 576
Sse9I AATT 9 cut(s) 305, 368, 463, 590, 845, 1039, 1048, 1243, 1268
SsiI CCGC 2 cut(s) 1134, 1353
SspI AATATT 1 cut(s) 962
SspMI CTAG 5 cut(s) 572, 882, 897, 1083, 1375
TaaI ACNGT 1 cut(s) 280
TaqI TCGA 1 cut(s) 919
TasI AATT 9 cut(s) 305, 368, 463, 590, 845, 1039, 1048, 1243, 1268
TatI WGTACW 2 cut(s) 318, 952
TfiI GAWTC 5 cut(s) 346, 384, 633, 671, 1183
Tru1I TTAA 3 cut(s) 462, 624, 1271
Tru9I TTAA 3 cut(s) 462, 624, 1271
TscAI CASTG 5 cut(s) 121, 283, 419, 612, 1218
TseI GCWGC 2 cut(s) 777, 1190
TspDTI ATGAA 7 cut(s) 33, 420, 491, 554, 582, 589, 711
TspRI CASTG 5 cut(s) 121, 283, 419, 612, 1218
Van91I CCANNNNNTGG 1 cut(s) 1217
VspI ATTAAT 1 cut(s) 1271
XapI RAATTY 2 cut(s) 590, 845
XbaI TCTAGA 1 cut(s) 1082
XceI RCATGY 3 cut(s) 342, 1081, 1134
XmiI GTMKAC 1 cut(s) 919
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 5 cut(s) 572, 882, 897, 1083, 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.