FvH4_3g45040

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004852
Physical Location & Seq
Forward (+)
37479231 .. 37480678
1448 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45040.t1

Sequence Viewer

Length: 843 bp
ATGTCCAGCACTAGTCAGAGCCAGAGCGAGAGGCGCTTGAGTGTCGTATATTACTGGGCGTCACAGTGGGACCCCGTTTCAGGTATTACACTACCTAACTTCGACAGAAGCAGGGCTCTGTTGAAATACATGTTCTTCAAGGTAACTCGTCCCTTCCGTCGTTGGATTGACCCACGAAGGGCTCACATGATTCCCATCCACCTAAAGCTCATCAAGCAGTCCCAAACCCGTTCCTCCATTCGTTCTCCGGTCGACATTGCTGAACCAAAGCTCTTCGGTGAACGACGGTTTATGGTCCCTATCTCAGAAGCCATAGCTGAATGGGATTTGTACAAGGAGGTCATTGCAGGGTATCTGTCCACCATGATTGCTGTTTCAGCAGATACCGAAAAATTTATCATGCAAATGATATACGAGGCAATTGAAAAGGCCGAGCCCGGTTGGCCTATAGACGTGGTCGTCATGTCCGTGACTGTTGACATTTTGGGCGTCGTTGTTCCAACAACTGAATTGTTCCACCGCCTGGACAATGTGAGACTTGATAGTTTGGAATCTAGTACTAGACAGAAGCCGTGTCGTATTTGCCGCAAGAGCCTTGATCATTTTCACGAGCTCGGAGAAGGGATTACTCGCTTGCCCTGCTCCCACATTTTTCATGTAGGTTGCTTTGTCCAGAGTTTGGAGCTGAGTCCTGTGCAAACCTGCCCCACCTGCGATCACCAACTCCATATTACGGGTGCACCAAGAGCCTCCTGGCCGTGGGATTGGCCTTTGTTTCTCATGGTCTCGGCAGGCTCTATGTTAACTGGTGCGCTTGTTGGCAGACTGTTAAGAAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

281

Amino Acids

32.09

Weight (kDa)

9.05

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 192 - 238 2.6e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 719
AasI GACNNNNNNGTC 1 cut(s) 458
Acc36I ACCTGC 2 cut(s) 710, 719
AccB7I CCANNNNNTGG 2 cut(s) 523, 679
AccI GTMKAC 1 cut(s) 252
AciI CCGC 2 cut(s) 520, 586
AcoI YGGCCR 1 cut(s) 755
AcsI RAATTY 1 cut(s) 392
AcyI GRCGYC 2 cut(s) 59, 489
AfaI GTAC 2 cut(s) 332, 559
AfiI CCNNNNNNNGG 6 cut(s) 80, 178, 523, 679, 733, 759
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 3 cut(s) 124, 139, 425
AhlI ACTAGT 1 cut(s) 11
AjiI CACGTC 1 cut(s) 454
AjnI CCWGG 2 cut(s) 522, 752
AluBI AGCT 5 cut(s) 208, 271, 317, 613, 685
AluI AGCT 5 cut(s) 208, 271, 317, 613, 685
Alw21I GWGCWC 2 cut(s) 615, 742
Alw26I GTCTC 2 cut(s) 529, 790
Alw44I GTGCAC 1 cut(s) 738
AoxI GGCC 4 cut(s) 429, 443, 755, 767
ApaLI GTGCAC 1 cut(s) 738
ApoI RAATTY 1 cut(s) 392
AspLEI GCGC 2 cut(s) 36, 814
AspS9I GGNCC 2 cut(s) 70, 295
AsuC2I CCSGG 1 cut(s) 438
AsuHPI GGTGA 2 cut(s) 290, 710
AvaII GGWCC 2 cut(s) 70, 295
BaeGI GKGCMC 1 cut(s) 742
BanII GRGCYC 4 cut(s) 118, 184, 438, 615
BauI CACGAG 1 cut(s) 608
Bbv12I GWGCWC 2 cut(s) 615, 742
BccI CCATC 1 cut(s) 203
BceAI ACGGC 2 cut(s) 556, 742
BciT130I CCWGG 2 cut(s) 524, 754
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 1 cut(s) 438
BcoDI GTCTC 2 cut(s) 529, 790
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 3 cut(s) 12, 555, 561
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 37
BfuAI ACCTGC 2 cut(s) 710, 719
BglI GCCNNNNNGGC 1 cut(s) 442
BisI GCNGC 1 cut(s) 586
BlsI GCNGC 1 cut(s) 587
BmcAI AGTACT 1 cut(s) 559
Bme1390I CCNGG 3 cut(s) 438, 524, 754
Bme18I GGWCC 2 cut(s) 70, 295
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 2 cut(s) 70, 295
BmiI GGNNCC 3 cut(s) 71, 72, 297
BmrFI CCNGG 3 cut(s) 438, 524, 754
BmrI ACTGGG 1 cut(s) 64
BmuI ACTGGG 1 cut(s) 64
BpuEI CTTGAG 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 438
BsaBI GATNNNNATC 1 cut(s) 194
BsaHI GRCGYC 2 cut(s) 59, 489
BsaI GGTCTC 1 cut(s) 790
BsaJI CCNNGG 1 cut(s) 758
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 178, 523, 679, 733, 759
Bse1I ACTGG 2 cut(s) 59, 811
Bse3DI GCAATG 2 cut(s) 255, 342
Bse8I GATNNNNATC 1 cut(s) 194
BseBI CCWGG 2 cut(s) 524, 754
BseDI CCNNGG 1 cut(s) 758
BseGI GGATG 1 cut(s) 195
BseJI GATNNNNATC 1 cut(s) 194
BseLI CCNNNNNNNGG 6 cut(s) 80, 178, 523, 679, 733, 759
BseMI GCAATG 2 cut(s) 255, 342
BseMII CTCAG 2 cut(s) 318, 677
BseNI ACTGG 2 cut(s) 59, 811
BseSI GKGCMC 1 cut(s) 742
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 4 cut(s) 431, 445, 757, 769
BsiEI CGRYCG 1 cut(s) 252
BsiHKAI GWGCWC 2 cut(s) 615, 742
BsiSI CCGG 2 cut(s) 248, 438
BslFI GGGAC 4 cut(s) 83, 135, 205, 281
BslI CCNNNNNNNGG 6 cut(s) 80, 178, 523, 679, 733, 759
BsmAI GTCTC 2 cut(s) 529, 790
BsmFI GGGAC 4 cut(s) 83, 135, 205, 281
BsnI GGCC 4 cut(s) 431, 445, 757, 769
Bso31I GGTCTC 1 cut(s) 790
Bsp1286I GDGCHC 5 cut(s) 118, 184, 438, 615, 742
Bsp1407I TGTACA 1 cut(s) 330
Bsp143I GATC 2 cut(s) 598, 715
BspACI CCGC 2 cut(s) 520, 586
BspANI GGCC 4 cut(s) 431, 445, 757, 769
BspCNI CTCAG 2 cut(s) 317, 678
BspLI GGNNCC 3 cut(s) 71, 72, 297
BspMI ACCTGC 2 cut(s) 710, 719
BspQI GCTCTTC 1 cut(s) 278
BspTNI GGTCTC 1 cut(s) 790
BsrDI GCAATG 2 cut(s) 255, 342
BsrGI TGTACA 1 cut(s) 330
BsrI ACTGG 2 cut(s) 59, 811
BssECI CCNNGG 1 cut(s) 758
BssMI GATC 2 cut(s) 598, 715
BssNI GRCGYC 2 cut(s) 59, 489
BssSI CACGAG 1 cut(s) 608
Bst2BI CACGAG 1 cut(s) 608
Bst2UI CCWGG 2 cut(s) 524, 754
Bst4CI ACNGT 4 cut(s) 66, 288, 475, 828
Bst6I CTCTTC 1 cut(s) 278
BstACI GRCGYC 2 cut(s) 59, 489
BstAUI TGTACA 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 635, 793
BstDEI CTNAG 2 cut(s) 304, 686
BstDSI CCRYGG 1 cut(s) 758
BstF5I GGATG 1 cut(s) 195
BstH2I RGCGCY 1 cut(s) 37
BstHHI GCGC 2 cut(s) 36, 814
BstKTI GATC 2 cut(s) 601, 718
BstMAI GTCTC 2 cut(s) 529, 790
BstMBI GATC 2 cut(s) 598, 715
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 8 cut(s) 33, 214, 377, 442, 591, 639, 711, 746
BstNI CCWGG 2 cut(s) 524, 754
BstNSI RCATGY 1 cut(s) 133
BstSCI CCNGG 3 cut(s) 436, 522, 752
BstSFI CTRYAG 1 cut(s) 447
BstSLI GKGCMC 1 cut(s) 742
BsuRI GGCC 4 cut(s) 431, 445, 757, 769
BtgI CCRYGG 1 cut(s) 758
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 71
BveI ACCTGC 2 cut(s) 710, 719
Cac8I GCNNGC 2 cut(s) 635, 793
CfoI GCGC 2 cut(s) 36, 814
Cfr13I GGNCC 2 cut(s) 70, 295
CseI GACGC 2 cut(s) 48, 478
Csp6I GTAC 2 cut(s) 331, 558
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 7 cut(s) 130, 187, 364, 400, 463, 656, 781
CviQI GTAC 2 cut(s) 331, 558
DdeI CTNAG 2 cut(s) 304, 686
DpnI GATC 2 cut(s) 600, 717
DpnII GATC 2 cut(s) 598, 715
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 1 cut(s) 755
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Ecl136II GAGCTC 1 cut(s) 613
Eco24I GRGCYC 4 cut(s) 118, 184, 438, 615
Eco31I GGTCTC 1 cut(s) 790
Eco47I GGWCC 2 cut(s) 70, 295
Eco53kI GAGCTC 1 cut(s) 613
EcoICRI GAGCTC 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 522, 752
EcoT38I GRGCYC 4 cut(s) 118, 184, 438, 615
FaeI CATG 7 cut(s) 133, 190, 367, 403, 466, 659, 784
FaqI GGGAC 4 cut(s) 83, 135, 205, 281
FatI CATG 7 cut(s) 129, 186, 363, 399, 462, 655, 780
FbaI TGATCA 1 cut(s) 598
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 1 cut(s) 586
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 4 cut(s) 118, 184, 438, 615
Fsp4HI GCNGC 1 cut(s) 586
FspBI CTAG 3 cut(s) 12, 555, 561
GlaI GCGC 2 cut(s) 35, 813
GluI GCNGC 1 cut(s) 586
HaeII RGCGCY 1 cut(s) 37
HaeIII GGCC 4 cut(s) 431, 445, 757, 769
HapII CCGG 2 cut(s) 248, 438
HgaI GACGC 2 cut(s) 48, 478
HhaI GCGC 2 cut(s) 36, 814
Hin1I GRCGYC 2 cut(s) 59, 489
Hin1II CATG 7 cut(s) 133, 190, 367, 403, 466, 659, 784
Hin6I GCGC 2 cut(s) 34, 812
HinP1I GCGC 2 cut(s) 34, 812
HincII GTYRAC 3 cut(s) 253, 478, 804
HindII GTYRAC 3 cut(s) 253, 478, 804
HinfI GANTC 3 cut(s) 190, 551, 688
HpaI GTTAAC 1 cut(s) 804
HpaII CCGG 2 cut(s) 248, 438
HphI GGTGA 2 cut(s) 290, 710
Hpy166II GTNNAC 6 cut(s) 253, 281, 360, 478, 740, 804
Hpy188I TCNGA 3 cut(s) 18, 307, 617
Hpy188III TCNNGA 2 cut(s) 608, 673
Hpy8I GTNNAC 6 cut(s) 253, 281, 360, 478, 740, 804
Hpy99I CGWCG 3 cut(s) 162, 288, 494
HpyAV CCTTC 3 cut(s) 163, 171, 614
HpyCH4III ACNGT 4 cut(s) 66, 288, 475, 828
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 4 cut(s) 347, 403, 697, 740
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 214, 377, 442, 591, 639, 711, 746
HpyF3I CTNAG 2 cut(s) 304, 686
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 2 cut(s) 59, 489
Hsp92II CATG 7 cut(s) 133, 190, 367, 403, 466, 659, 784
HspAI GCGC 2 cut(s) 34, 812
KflI GGGWCCC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 598
KspAI GTTAAC 1 cut(s) 804
Kzo9I GATC 2 cut(s) 598, 715
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 2 cut(s) 647, 682
MaeI CTAG 3 cut(s) 12, 555, 561
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 60, 142, 469
MalI GATC 2 cut(s) 600, 717
MboI GATC 2 cut(s) 598, 715
MboII GAAGA 2 cut(s) 127, 265
MfeI CAATTG 1 cut(s) 420
MhlI GDGCHC 5 cut(s) 118, 184, 438, 615, 742
MluCI AATT 3 cut(s) 392, 420, 509
MlyI GAGTC 1 cut(s) 697
MmeI TCCRAC 2 cut(s) 143, 524
MnlI CCTC 5 cut(s) 24, 244, 331, 409, 760
MseI TTAA 2 cut(s) 803, 830
MslI CAYNNNNRTG 2 cut(s) 404, 467
MspI CCGG 2 cut(s) 248, 438
MspR9I CCNGG 3 cut(s) 438, 524, 754
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 2 cut(s) 524, 754
MwoI GCNNNNNNNGC 8 cut(s) 33, 214, 377, 442, 591, 639, 711, 746
NciI CCSGG 1 cut(s) 438
NdeII GATC 2 cut(s) 598, 715
NlaIII CATG 7 cut(s) 133, 190, 367, 403, 466, 659, 784
NlaIV GGNNCC 3 cut(s) 71, 72, 297
NmeAIII GCCGAG 2 cut(s) 457, 767
NmuCI GTSAC 2 cut(s) 60, 469
NspI RCATGY 1 cut(s) 133
PaqCI CACCTGC 1 cut(s) 719
PciI ACATGT 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 2 cut(s) 154, 465
PfeI GAWTC 2 cut(s) 190, 551
PflFI GACNNNGTC 1 cut(s) 455
PflMI CCANNNNNTGG 2 cut(s) 523, 679
PkrI GCNGC 1 cut(s) 587
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
PpuMI RGGWCCY 1 cut(s) 70
PscI ACATGT 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 522, 752
PspGI CCWGG 2 cut(s) 522, 752
PspN4I GGNNCC 3 cut(s) 71, 72, 297
PspPI GGNCC 2 cut(s) 70, 295
PspPPI RGGWCCY 1 cut(s) 70
PsyI GACNNNGTC 1 cut(s) 455
RsaI GTAC 2 cut(s) 332, 559
RsaNI GTAC 2 cut(s) 331, 558
RseI CAYNNNNRTG 2 cut(s) 404, 467
SacI GAGCTC 1 cut(s) 615
SalI GTCGAC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 803, 830
SatI GCNGC 1 cut(s) 586
Sau3AI GATC 2 cut(s) 598, 715
Sau96I GGNCC 2 cut(s) 70, 295
ScaI AGTACT 1 cut(s) 559
SchI GAGTC 1 cut(s) 697
ScrFI CCNGG 3 cut(s) 438, 524, 754
SduI GDGCHC 5 cut(s) 118, 184, 438, 615, 742
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 2 cut(s) 70, 295
SmiMI CAYNNNNRTG 2 cut(s) 404, 467
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 3 cut(s) 392, 420, 509
SsiI CCGC 2 cut(s) 520, 586
SspMI CTAG 3 cut(s) 12, 555, 561
SstI GAGCTC 1 cut(s) 615
StyD4I CCNGG 3 cut(s) 436, 522, 752
TaaI ACNGT 4 cut(s) 66, 288, 475, 828
TaiI ACGT 1 cut(s) 456
TaqI TCGA 2 cut(s) 102, 252
TasI AATT 3 cut(s) 392, 420, 509
TatI WGTACW 2 cut(s) 330, 557
TauI GCSGC 1 cut(s) 588
TfiI GAWTC 2 cut(s) 190, 551
Tru1I TTAA 2 cut(s) 803, 830
Tru9I TTAA 2 cut(s) 803, 830
TscAI CASTG 1 cut(s) 71
TseFI GTSAC 2 cut(s) 60, 469
Tsp45I GTSAC 2 cut(s) 60, 469
TspDTI ATGAA 1 cut(s) 644
TspGWI ACGGA 2 cut(s) 146, 457
TspRI CASTG 1 cut(s) 71
Tth111I GACNNNGTC 1 cut(s) 455
Van91I CCANNNNNTGG 2 cut(s) 523, 679
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 2 cut(s) 70, 295
XapI RAATTY 1 cut(s) 392
XceI RCATGY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 1 cut(s) 750
XmiI GTMKAC 1 cut(s) 252
XspI CTAG 3 cut(s) 12, 555, 561
ZrmI AGTACT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.