Rorug05G0480700

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001691
Physical Location & Seq
Forward (+)
66200772 .. 66203276
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0480700.1

Sequence Viewer

Length: 750 bp
ATGGCGATTCGTGCATTTTCAAGATCAAAATTCCCAACTTTCCCTTCAATACTCCTTCAAGGTCTAAAACGAACATCAATCCGAGAGAACCCATTTCCTCCAAAATCCACTTCTCCAGGACTAGAAATCGAACCGTGGAAACCCATTTGTGGGTTTAGGCTATACCATGATGGGAGGCCTAGAGGTCCCCTTTGGAGGGGTAAGAAGCTGATTGGCAAAGAAGCCCTATATGTGATTTCGGGTTTGAAGCGGTTTAAGGATGACGAAGAGAGGCTTGGCAAGTTTATAAAGAACCATGTTTTGAGGCTTCTCAAGATGGATATGATTGCTGTTCTCACTGAGCTTGAGCGTCAGGAAGAGGTATTTAAGGTGACTCGAAAGCAGGATTGGTACAAGCCTGATGTCTATCTTTATAAGGACTTGATCATTTCATTAGCAAAATCTAAGAAAATGGATGATGTTATGCTGCTGTGGGAGTGCATGAAAAAGGAGGATCTGTTTCCTGATTCACAAACATATACAGAAGTTATCAGGGGATTTTTGAGCTCTGGTTCCCCTGCTGATGCAATGAACATATACGAGGACATGAAACAGTCTCCAGATCCACCAGAGGAGCTACCATTCCGGATTTTGTTAAAGGGACTTTTGCCGCATCCCCTTTTGAGAAACAGAGTCAAGCAAGACTTTGAGGAGCTCTTTCCTGAACAACATGTGTATGACCCTCCGCAAGAGATTTTTGGCTCCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

249

Amino Acids

29.15

Weight (kDa)

8.95

Isoelectric Point (pI)

65.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 133 - 180 1.9e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 287, 414
AccIII TCCGGA 1 cut(s) 624
AciI CCGC 3 cut(s) 250, 650, 725
AclWI GGATC 2 cut(s) 501, 596
AcsI RAATTY 1 cut(s) 29
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 4 cut(s) 149, 150, 195, 196
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 4 cut(s) 21, 48, 59, 247
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 8 cut(s) 28, 60, 94, 126, 258, 290, 370, 402
AluBI AGCT 5 cut(s) 208, 343, 546, 616, 694
AluI AGCT 5 cut(s) 208, 343, 546, 616, 694
Alw21I GWGCWC 2 cut(s) 548, 696
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 2 cut(s) 501, 596
Aor13HI TCCGGA 1 cut(s) 624
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 1 cut(s) 466
ApoI RAATTY 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 382
AvaII GGWCC 1 cut(s) 185
BanII GRGCYC 2 cut(s) 548, 696
Bbv12I GWGCWC 2 cut(s) 548, 696
BbvI GCAGC 1 cut(s) 453
BccI CCATC 2 cut(s) 164, 310
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 2 cut(s) 122, 180
BisI GCNGC 2 cut(s) 467, 650
BlsI GCNGC 2 cut(s) 468, 651
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 3 cut(s) 187, 553, 742
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 553, 661
BpmI CTGGAG 2 cut(s) 99, 582
BpuEI CTTGAG 2 cut(s) 296, 365
BsaBI GATNNNNATC 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 134
BsaWI WCCGGW 1 cut(s) 624
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 150, 195, 196
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 405
BseAI TCCGGA 1 cut(s) 624
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 3 cut(s) 265, 460, 652
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 4 cut(s) 149, 150, 195, 196
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 1 cut(s) 330
BseRI GAGGAG 2 cut(s) 626, 704
BseXI GCAGC 1 cut(s) 453
BshFI GGCC 1 cut(s) 178
BsiHKAI GWGCWC 2 cut(s) 548, 696
BsiSI CCGG 1 cut(s) 625
BslFI GGGAC 2 cut(s) 171, 654
BslI CCNNNNNNNGG 4 cut(s) 149, 150, 195, 196
BsmAI GTCTC 1 cut(s) 600
BsmFI GGGAC 2 cut(s) 171, 654
BsnI GGCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 548, 696
Bsp13I TCCGGA 1 cut(s) 624
Bsp143I GATC 4 cut(s) 23, 423, 493, 601
BspACI CCGC 3 cut(s) 250, 650, 725
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 1 cut(s) 331
BspEI TCCGGA 1 cut(s) 624
BspLI GGNNCC 3 cut(s) 187, 553, 742
BspPI GGATC 2 cut(s) 501, 596
BsrDI GCAATG 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 4 cut(s) 23, 423, 493, 601
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 135, 594
Bst6I CTCTTC 2 cut(s) 261, 351
BstDEI CTNAG 2 cut(s) 339, 444
BstDSI CCRYGG 1 cut(s) 134
BstF5I GGATG 3 cut(s) 265, 460, 652
BstKTI GATC 4 cut(s) 26, 426, 496, 604
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 4 cut(s) 23, 423, 493, 601
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 713
BstSCI CCNGG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 453
BstX2I RGATCY 2 cut(s) 493, 601
BstYI RGATCY 2 cut(s) 493, 601
BsuRI GGCC 1 cut(s) 178
BtgI CCRYGG 1 cut(s) 134
BtsCI GGATG 3 cut(s) 265, 460, 652
BtsIMutI CAGTG 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 185
CseI GACGC 1 cut(s) 338
Csp6I GTAC 1 cut(s) 391
CviAII CATG 5 cut(s) 167, 296, 481, 586, 710
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 2 cut(s) 339, 444
DpnI GATC 4 cut(s) 25, 425, 495, 603
DpnII GATC 4 cut(s) 23, 423, 493, 601
Eam1104I CTCTTC 2 cut(s) 261, 351
EarI CTCTTC 2 cut(s) 261, 351
Ecl136II GAGCTC 2 cut(s) 546, 694
Eco147I AGGCCT 1 cut(s) 178
Eco24I GRGCYC 2 cut(s) 548, 696
Eco47I GGWCC 1 cut(s) 185
Eco53kI GAGCTC 2 cut(s) 546, 694
EcoICRI GAGCTC 2 cut(s) 546, 694
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 115
EcoT38I GRGCYC 2 cut(s) 548, 696
FaeI CATG 5 cut(s) 170, 299, 484, 589, 713
FalI AAGNNNNNCTT 4 cut(s) 258, 290, 668, 700
FaqI GGGAC 2 cut(s) 171, 654
FatI CATG 5 cut(s) 166, 295, 480, 585, 709
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 467, 650
FokI GGATG 3 cut(s) 272, 467, 639
FriOI GRGCYC 2 cut(s) 548, 696
Fsp4HI GCNGC 2 cut(s) 467, 650
FspBI CTAG 2 cut(s) 122, 180
GluI GCNGC 2 cut(s) 467, 650
GsuI CTGGAG 2 cut(s) 99, 582
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 625
HgaI GACGC 1 cut(s) 338
Hin1II CATG 5 cut(s) 170, 299, 484, 589, 713
HinfI GANTC 4 cut(s) 7, 373, 506, 672
HpaII CCGG 1 cut(s) 625
HphI GGTGA 1 cut(s) 382
Hpy188I TCNGA 1 cut(s) 83
Hpy188III TCNNGA 7 cut(s) 21, 313, 353, 503, 599, 625, 701
HpyAV CCTTC 2 cut(s) 54, 65
HpyCH4III ACNGT 2 cut(s) 135, 594
HpyCH4V TGCA 3 cut(s) 14, 480, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 339, 444
Hsp92II CATG 5 cut(s) 170, 299, 484, 589, 713
Kpn2I TCCGGA 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 4 cut(s) 23, 423, 493, 601
LmnI GCTCC 3 cut(s) 613, 691, 746
Lsp1109I GCAGC 1 cut(s) 453
LweI GCATC 2 cut(s) 553, 661
MaeI CTAG 2 cut(s) 122, 180
MaeIII GTNAC 1 cut(s) 370
MalI GATC 4 cut(s) 25, 425, 495, 603
MboI GATC 4 cut(s) 23, 423, 493, 601
MboII GAAGA 2 cut(s) 278, 368
MflI RGATCY 2 cut(s) 493, 601
MhlI GDGCHC 2 cut(s) 548, 696
MluCI AATT 1 cut(s) 29
MlyI GAGTC 2 cut(s) 367, 681
MroI TCCGGA 1 cut(s) 624
MseI TTAA 3 cut(s) 255, 366, 635
MslI CAYNNNNRTG 1 cut(s) 714
MspI CCGG 1 cut(s) 625
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 4 cut(s) 23, 423, 493, 601
NlaIII CATG 5 cut(s) 170, 299, 484, 589, 713
NlaIV GGNNCC 3 cut(s) 187, 553, 742
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 1 cut(s) 713
PceI AGGCCT 1 cut(s) 178
PciI ACATGT 1 cut(s) 709
PfeI GAWTC 2 cut(s) 7, 506
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 2 cut(s) 468, 651
PleI GAGTC 2 cut(s) 367, 680
PpsI GAGTC 2 cut(s) 367, 680
PpuMI RGGWCCY 1 cut(s) 185
PscI ACATGT 1 cut(s) 709
PsiI TTATAA 2 cut(s) 287, 414
Psp124BI GAGCTC 2 cut(s) 548, 696
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 187, 553, 742
PspPI GGNCC 1 cut(s) 185
PspPPI RGGWCCY 1 cut(s) 185
PsuI RGATCY 2 cut(s) 493, 601
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 714
SacI GAGCTC 2 cut(s) 548, 696
SaqAI TTAA 3 cut(s) 255, 366, 635
SatI GCNGC 2 cut(s) 467, 650
Sau3AI GATC 4 cut(s) 23, 423, 493, 601
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 2 cut(s) 367, 681
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 2 cut(s) 548, 696
SetI ASST 9 cut(s) 64, 187, 210, 345, 363, 372, 548, 618, 696
SfaNI GCATC 2 cut(s) 553, 661
SinI GGWCC 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 2 cut(s) 311, 344
SmoI CTYRAG 2 cut(s) 311, 344
Sse9I AATT 1 cut(s) 29
SseBI AGGCCT 1 cut(s) 178
SsiI CCGC 3 cut(s) 250, 650, 725
SspMI CTAG 2 cut(s) 122, 180
SstI GAGCTC 2 cut(s) 548, 696
StuI AGGCCT 1 cut(s) 178
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 135, 594
TaqI TCGA 2 cut(s) 129, 376
TasI AATT 1 cut(s) 29
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 2 cut(s) 7, 506
Tru1I TTAA 3 cut(s) 255, 366, 635
Tru9I TTAA 3 cut(s) 255, 366, 635
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 466
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 4 cut(s) 420, 497, 584, 602
TspRI CASTG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 29
XceI RCATGY 1 cut(s) 713
XspI CTAG 2 cut(s) 122, 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.