Rorug07G0271500

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001691
Physical Location & Seq
Reverse (-)
25675083 .. 25676806
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0271500.1

Sequence Viewer

Length: 1095 bp
ATGATGGCGATGACTCGCACGCAAGCGTGTAGGCACATGGCGAAGTCGGCGGCGCACGTAGCAAGTGCGCGCCTCTTCTCCACGGCAGCCAGAGCTGCCACCACTAATGCGCCCACGGCCAAACAATTGTCCTCCATAATCATTCCTCTAAAGGATGCGGCCGCAGGCGCATGGTGGAACAACGGCAGCATCATTTACCTCGTTCAGAGGGTACGCAGTGGTAGCACTCTGGCTCTCGGCCGTCAGGAGAAGCAGCAGGAGGAGGAGGCTGATCATAGCAACTCCGCCGTTTCAGTTGCCGGACACGGTGGTGGTAATAAGGACGATGAGGAGAAGGCCATCACCAGCTACTGGGGGGTGGGACCGAACAAGATTACCAAGGAAGACGGTACTGAATGGAAATGGAATTGCTTCAGACCATGGGAGACGTACAAGGCCGATACCTCGATAGATCTCCAGAAGCATCACGTGCCTACAACTTTGATGGACAAAATGGCCTACTGGACTGTCAAGTCTTTCCGATGGCCTACGGACCTATTCTTCCAGAGGCGATATGGGTGCAGGGCAATGATGCTGGAGACAGTGGCAGCAGTGCCTGGAATGGTAGGAGGGATGCTGCTGCACTGCAAATCGCTTAGGCGATTCGAGCACAGTGGCGGCTGGATCAAAGCTCTTCTAGAAGAAGCCGAGAACGAGCGTATGCACCTCATGACCTTCATGGAAGTCGCCAAGCCCAGGTGGTACGAGCGCGCGCTTGTATTTGCCGTTCAAGGTGTTTTCTTCAATGCGTACTTCTTGGGATATATGATTTCACCCAAGTTTGCTCATAGAATGGTTGGATATCTTGAGGAGGAAGCAATTCACTCATATACCGAGTTCCTCAAGGAGTTAGACAAAGGTAACATTGAGAATGTTCCAGCTCCAGCTATTGCTATCGATTATTGGCAGCTACCACCCAATTCCACCTTGAGAGATGTTGTTATAGTTGTTAGGGCAGACGAGGCTCACCACCGAGATGTCAATCACTTCGCTTCGGACATACATTATAATGGACGTGAACTGAAGGAATCTCCCGCTCCAATTGGTTACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

364

Amino Acids

41.21

Weight (kDa)

7.77

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 153 - 345 7.2e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1047
AasI GACNNNNNNGTC 1 cut(s) 511
AccB7I CCANNNNNTGG 1 cut(s) 351
AccBSI CCGCTC 1 cut(s) 1076
AccII CGCG 3 cut(s) 70, 750, 752
AciI CCGC 6 cut(s) 50, 158, 162, 285, 657, 1074
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 3 cut(s) 117, 159, 238
AcuI CTGAAG 2 cut(s) 397, 1082
AcvI CACGTG 1 cut(s) 469
AfaI GTAC 5 cut(s) 213, 391, 431, 743, 791
AfiI CCNNNNNNNGG 2 cut(s) 351, 735
AgsI TTSAA 2 cut(s) 770, 784
AjiI CACGTC 1 cut(s) 1055
AjnI CCWGG 2 cut(s) 595, 734
AleI CACNNNNGTG 1 cut(s) 309
AluBI AGCT 6 cut(s) 95, 348, 671, 920, 926, 949
AluI AGCT 6 cut(s) 95, 348, 671, 920, 926, 949
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 2 cut(s) 419, 572
AlwI GGATC 1 cut(s) 671
AlwNI CAGNNNCTG 2 cut(s) 351, 596
AoxI GGCC 7 cut(s) 117, 159, 238, 336, 435, 495, 524
ApeKI GCWGC 8 cut(s) 86, 95, 186, 253, 587, 616, 619, 946
Asp700I GAANNNNTTC 2 cut(s) 410, 858
AspLEI GCGC 8 cut(s) 55, 70, 72, 112, 170, 750, 752, 754
AspS9I GGNCC 2 cut(s) 362, 532
AsuHPI GGTGA 3 cut(s) 334, 804, 998
AvaII GGWCC 2 cut(s) 362, 532
BbrPI CACGTG 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 8 cut(s) 82, 98, 198, 265, 599, 603, 606, 958
BccI CCATC 3 cut(s) 347, 478, 516
BceAI ACGGC 6 cut(s) 99, 132, 199, 225, 272, 749
BcgI CGANNNNNNTGC 2 cut(s) 540, 574
BciT130I CCWGG 2 cut(s) 597, 736
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 419, 572
BfaI CTAG 1 cut(s) 677
BglII AGATCT 1 cut(s) 451
Bme1390I CCNGG 2 cut(s) 597, 736
Bme18I GGWCC 2 cut(s) 362, 532
BmgBI CACGTC 1 cut(s) 1055
BmgT120I GGNCC 2 cut(s) 362, 532
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 2 cut(s) 597, 736
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 5 cut(s) 145, 198, 472, 561, 603
BmuI ACTGGG 1 cut(s) 361
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 3 cut(s) 440, 596, 906
Bpu10I CCTNAGC 1 cut(s) 635
BpuEI CTTGAG 3 cut(s) 866, 866, 988
Bsa29I ATCGAT 1 cut(s) 936
BsaAI YACGTR 2 cut(s) 58, 469
BsaBI GATNNNNATC 1 cut(s) 1020
BsaJI CCNNGG 5 cut(s) 81, 114, 378, 419, 734
Bsc4I CCNNNNNNNGG 2 cut(s) 351, 735
Bse1I ACTGG 2 cut(s) 356, 506
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 1020
BseBI CCWGG 2 cut(s) 597, 736
BseCI ATCGAT 1 cut(s) 936
BseDI CCNNGG 5 cut(s) 81, 114, 378, 419, 734
BseGI GGATG 2 cut(s) 160, 618
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 2 cut(s) 351, 735
BseMI GCAATG 1 cut(s) 573
BseNI ACTGG 2 cut(s) 356, 506
BsePI GCGCGC 3 cut(s) 68, 748, 750
BseRI GAGGAG 4 cut(s) 275, 278, 344, 863
BseX3I CGGCCG 2 cut(s) 159, 238
BseXI GCAGC 8 cut(s) 82, 98, 198, 265, 599, 603, 606, 958
BsgI GTGCAG 2 cut(s) 580, 605
Bsh1236I CGCG 3 cut(s) 70, 750, 752
Bsh1285I CGRYCG 2 cut(s) 162, 241
BshFI GGCC 7 cut(s) 119, 161, 240, 338, 437, 497, 526
BshVI ATCGAT 1 cut(s) 936
BsiEI CGRYCG 2 cut(s) 162, 241
BsiHKAI GWGCWC 1 cut(s) 651
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 351, 735
BsmAI GTCTC 2 cut(s) 419, 572
BsmBI CGTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 7 cut(s) 119, 161, 240, 338, 437, 497, 526
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 3 cut(s) 271, 451, 663
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 6 cut(s) 50, 158, 162, 285, 657, 1074
BspANI GGCC 7 cut(s) 119, 161, 240, 338, 437, 497, 526
BspDI ATCGAT 1 cut(s) 936
BspFNI CGCG 3 cut(s) 70, 750, 752
BspHI TCATGA 1 cut(s) 708
BspLI GGNNCC 1 cut(s) 363
BspPI GGATC 1 cut(s) 671
BspQI GCTCTTC 1 cut(s) 678
BsrBI CCGCTC 1 cut(s) 1076
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 2 cut(s) 356, 506
BssECI CCNNGG 5 cut(s) 81, 114, 378, 419, 734
BssHII GCGCGC 3 cut(s) 68, 748, 750
BssMI GATC 3 cut(s) 271, 451, 663
BssT1I CCWWGG 2 cut(s) 378, 419
Bst2UI CCWGG 2 cut(s) 597, 736
Bst4CI ACNGT 5 cut(s) 308, 389, 508, 583, 653
Bst6I CTCTTC 2 cut(s) 80, 678
BstAPI GCANNNNNTGC 1 cut(s) 469
BstBAI YACGTR 2 cut(s) 58, 469
BstC8I GCNNGC 6 cut(s) 20, 24, 70, 166, 750, 752
BstDEI CTNAG 1 cut(s) 635
BstDSI CCRYGG 3 cut(s) 81, 114, 419
BstEII GGTNACC 1 cut(s) 1085
BstF5I GGATG 2 cut(s) 160, 618
BstFNI CGCG 3 cut(s) 70, 750, 752
BstHHI GCGC 8 cut(s) 55, 70, 72, 112, 170, 750, 752, 754
BstKTI GATC 3 cut(s) 274, 454, 666
BstMAI GTCTC 2 cut(s) 419, 572
BstMBI GATC 3 cut(s) 271, 451, 663
BstMCI CGRYCG 2 cut(s) 162, 241
BstNI CCWGG 2 cut(s) 597, 736
BstPI GGTNACC 1 cut(s) 1085
BstSCI CCNGG 2 cut(s) 595, 734
BstUI CGCG 3 cut(s) 70, 750, 752
BstV1I GCAGC 8 cut(s) 82, 98, 198, 265, 599, 603, 606, 958
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BstZI CGGCCG 2 cut(s) 159, 238
Bsu15I ATCGAT 1 cut(s) 936
BsuRI GGCC 7 cut(s) 119, 161, 240, 338, 437, 497, 526
BsuTUI ATCGAT 1 cut(s) 936
BtgI CCRYGG 3 cut(s) 81, 114, 419
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 1055
BtsCI GGATG 2 cut(s) 160, 618
BtsI GCAGTG 3 cut(s) 223, 597, 622
BtsIMutI CAGTG 5 cut(s) 223, 588, 597, 622, 658
Cac8I GCNNGC 6 cut(s) 20, 24, 70, 166, 750, 752
CaiI CAGNNNCTG 2 cut(s) 351, 596
CciI TCATGA 1 cut(s) 708
CciNI GCGGCCGC 1 cut(s) 159
CfoI GCGC 8 cut(s) 55, 70, 72, 112, 170, 750, 752, 754
Cfr13I GGNCC 2 cut(s) 362, 532
ClaI ATCGAT 1 cut(s) 936
Csp6I GTAC 5 cut(s) 212, 390, 430, 742, 790
CviAII CATG 5 cut(s) 37, 171, 420, 709, 718
CviQI GTAC 5 cut(s) 212, 390, 430, 742, 790
DdeI CTNAG 1 cut(s) 635
DpnI GATC 3 cut(s) 273, 453, 665
DpnII GATC 3 cut(s) 271, 451, 663
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 3 cut(s) 117, 159, 238
EagI CGGCCG 2 cut(s) 159, 238
Eam1104I CTCTTC 2 cut(s) 80, 678
EarI CTCTTC 2 cut(s) 80, 678
EciI GGCGGA 1 cut(s) 274
EclXI CGGCCG 2 cut(s) 159, 238
Eco130I CCWWGG 2 cut(s) 378, 419
Eco32I GATATC 1 cut(s) 842
Eco47I GGWCC 2 cut(s) 362, 532
Eco52I CGGCCG 2 cut(s) 159, 238
Eco57I CTGAAG 2 cut(s) 397, 1082
Eco72I CACGTG 1 cut(s) 469
Eco91I GGTNACC 1 cut(s) 1085
EcoO65I GGTNACC 1 cut(s) 1085
EcoRII CCWGG 2 cut(s) 595, 734
EcoRV GATATC 1 cut(s) 842
EcoT14I CCWWGG 2 cut(s) 378, 419
ErhI CCWWGG 2 cut(s) 378, 419
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 5 cut(s) 40, 174, 423, 712, 721
FaqI GGGAC 1 cut(s) 375
FatI CATG 5 cut(s) 36, 170, 419, 708, 717
FauI CCCGC 1 cut(s) 1081
FbaI TGATCA 1 cut(s) 271
FokI GGATG 2 cut(s) 167, 625
FspBI CTAG 1 cut(s) 677
GlaI GCGC 8 cut(s) 54, 69, 71, 111, 169, 749, 751, 753
GsuI CTGGAG 3 cut(s) 440, 596, 906
HaeIII GGCC 7 cut(s) 119, 161, 240, 338, 437, 497, 526
HapII CCGG 1 cut(s) 300
HhaI GCGC 8 cut(s) 55, 70, 72, 112, 170, 750, 752, 754
Hin1II CATG 5 cut(s) 40, 174, 423, 712, 721
Hin6I GCGC 8 cut(s) 53, 68, 70, 110, 168, 748, 750, 752
HinP1I GCGC 8 cut(s) 53, 68, 70, 110, 168, 748, 750, 752
HinfI GANTC 3 cut(s) 13, 642, 1067
HpaII CCGG 1 cut(s) 300
HphI GGTGA 3 cut(s) 334, 804, 998
Hpy166II GTNNAC 1 cut(s) 1058
Hpy188I TCNGA 4 cut(s) 207, 416, 521, 1036
Hpy188III TCNNGA 6 cut(s) 245, 457, 544, 677, 709, 845
Hpy8I GTNNAC 1 cut(s) 1058
HpyAV CCTTC 3 cut(s) 328, 724, 1057
HpyCH4III ACNGT 5 cut(s) 308, 389, 508, 583, 653
HpyCH4IV ACGT 4 cut(s) 57, 428, 468, 1054
HpyCH4V TGCA 4 cut(s) 561, 622, 627, 703
HpyF3I CTNAG 1 cut(s) 635
HpySE526I ACGT 4 cut(s) 57, 428, 468, 1054
Hsp92II CATG 5 cut(s) 40, 174, 423, 712, 721
HspAI GCGC 8 cut(s) 53, 68, 70, 110, 168, 748, 750, 752
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 3 cut(s) 271, 451, 663
LguI GCTCTTC 1 cut(s) 678
LmnI GCTCC 2 cut(s) 925, 1081
Lsp1109I GCAGC 8 cut(s) 82, 98, 198, 265, 599, 603, 606, 958
LweI GCATC 5 cut(s) 145, 198, 472, 561, 603
MaeI CTAG 1 cut(s) 677
MaeII ACGT 4 cut(s) 57, 428, 468, 1054
MaeIII GTNAC 2 cut(s) 899, 1085
MalI GATC 3 cut(s) 273, 453, 665
MbiI CCGCTC 1 cut(s) 1076
MboI GATC 3 cut(s) 271, 451, 663
MboII GAAGA 6 cut(s) 67, 395, 532, 665, 692, 772
MfeI CAATTG 2 cut(s) 125, 1080
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 5 cut(s) 125, 406, 858, 958, 1080
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 817
MroXI GAANNNNTTC 2 cut(s) 410, 858
MslI CAYNNNNRTG 3 cut(s) 309, 1014, 1047
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 597, 736
MunI CAATTG 2 cut(s) 125, 1080
MvaI CCWGG 2 cut(s) 597, 736
MvnI CGCG 3 cut(s) 70, 750, 752
NcoI CCATGG 1 cut(s) 419
NdeII GATC 3 cut(s) 271, 451, 663
NlaIII CATG 5 cut(s) 40, 174, 423, 712, 721
NlaIV GGNNCC 1 cut(s) 363
NmeAIII GCCGAG 2 cut(s) 216, 712
NotI GCGGCCGC 1 cut(s) 159
OliI CACNNNNGTG 1 cut(s) 309
PagI TCATGA 1 cut(s) 708
PauI GCGCGC 3 cut(s) 68, 748, 750
PciSI GCTCTTC 1 cut(s) 678
PdmI GAANNNNTTC 2 cut(s) 410, 858
PfeI GAWTC 2 cut(s) 642, 1067
PflMI CCANNNNNTGG 1 cut(s) 351
PleI GAGTC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 469
PmlI CACGTG 1 cut(s) 469
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 2 cut(s) 58, 469
PsiI TTATAA 1 cut(s) 1047
Psp6I CCWGG 2 cut(s) 595, 734
PspCI CACGTG 1 cut(s) 469
PspEI GGTNACC 1 cut(s) 1085
PspGI CCWGG 2 cut(s) 595, 734
PspN4I GGNNCC 1 cut(s) 363
PspPI GGNCC 2 cut(s) 362, 532
PsrI GAACNNNNNNTAC 2 cut(s) 750, 782
PstNI CAGNNNCTG 2 cut(s) 351, 596
PsuI RGATCY 1 cut(s) 451
PteI GCGCGC 3 cut(s) 68, 748, 750
RsaI GTAC 5 cut(s) 213, 391, 431, 743, 791
RsaNI GTAC 5 cut(s) 212, 390, 430, 742, 790
RseI CAYNNNNRTG 3 cut(s) 309, 1014, 1047
SapI GCTCTTC 1 cut(s) 678
Sau3AI GATC 3 cut(s) 271, 451, 663
Sau96I GGNCC 2 cut(s) 362, 532
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 597, 736
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 5 cut(s) 145, 198, 472, 561, 603
SinI GGWCC 2 cut(s) 362, 532
SmiMI CAYNNNNRTG 3 cut(s) 309, 1014, 1047
SmlI CTYRAG 3 cut(s) 845, 881, 967
SmoI CTYRAG 3 cut(s) 845, 881, 967
Sse9I AATT 5 cut(s) 125, 406, 858, 958, 1080
SsiI CCGC 6 cut(s) 50, 158, 162, 285, 657, 1074
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 2 cut(s) 595, 734
StyI CCWWGG 2 cut(s) 378, 419
TaaI ACNGT 5 cut(s) 308, 389, 508, 583, 653
TaiI ACGT 4 cut(s) 60, 431, 471, 1057
TaqI TCGA 3 cut(s) 446, 645, 936
TaqII GACCGA 1 cut(s) 379
TasI AATT 5 cut(s) 125, 406, 858, 958, 1080
TauI GCSGC 4 cut(s) 53, 161, 164, 660
TfiI GAWTC 2 cut(s) 642, 1067
TscAI CASTG 5 cut(s) 223, 588, 597, 629, 658
TseI GCWGC 8 cut(s) 86, 95, 186, 253, 587, 616, 619, 946
TspDTI ATGAA 1 cut(s) 706
TspGWI ACGGA 1 cut(s) 545
TspRI CASTG 5 cut(s) 223, 588, 597, 629, 658
Van91I CCANNNNNTGG 1 cut(s) 351
VpaK11BI GGWCC 2 cut(s) 362, 532
XbaI TCTAGA 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XmnI GAANNNNTTC 2 cut(s) 410, 858
XspI CTAG 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.