Rroxscaffold_6G00424730
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004852
Physical Location & Seq
Forward (+)
45870606 .. 45876866
6261 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_6G00424730.1

Sequence Viewer

Length: 546 bp
ATGTACATGTCAAGAAACAAGCTTTCTGGGAGAATTCCAGTGACCCTCTCCAATCTTACACAGCTGATACTGTTTGATTCTCGGACACTTAAGCGGCCTGGTAACCTTGTACTTGTCGTACACTCGTACAACCGTTTGGAAGGAAAAATTCCATCTTCTCTTGGCAAGCTTTCGAATTTGCAGAGGTCGTACTTGGGGAACAACAGAATTATTGGGCCAATGCCAGATGACTTAGGGATGATGTCTAACCTTGGTGTACTAGAATTTGGCAACAATTCGATTGGTGGGCCAATTCCGCCTTCACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCTAGAAATCGCCTATCAGAAAAGTTTCCAATTGAGCTTACTCACTGTTCTCTTATGATGCTGCTTGATCTATCTTTCAACAACTTACAAGGCTCTGTTCCTGGAAGTAGAGAGATGTTGGTGGAGCGAGGCTCGCTTAAATACCCCGATCTCAATTACTTTCCTAGACATCATACTTCATTGGGTACGCCACATGAGAAAGAGTAG

Protein Analysis

181

Amino Acids

20.33

Weight (kDa)

9.76

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 59 - 118 9.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 94, 296
AcsI RAATTY 4 cut(s) 33, 147, 175, 263
AfaI GTAC 7 cut(s) 5, 111, 120, 128, 191, 258, 526
AfiI CCNNNNNNNGG 1 cut(s) 305
AflII CTTAAG 1 cut(s) 89
AflIII ACRYGT 1 cut(s) 6
AgsI TTSAA 1 cut(s) 418
AjnI CCWGG 2 cut(s) 97, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 5 cut(s) 22, 64, 169, 322, 376
AluI AGCT 5 cut(s) 22, 64, 169, 322, 376
AoxI GGCC 3 cut(s) 95, 215, 287
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 4 cut(s) 33, 147, 175, 263
Asp700I GAANNNNTTC 1 cut(s) 363
AspS9I GGNCC 2 cut(s) 215, 287
AsuII TTCGAA 1 cut(s) 173
BbvI GCAGC 1 cut(s) 387
BccI CCATC 1 cut(s) 160
BciT130I CCWGG 2 cut(s) 99, 441
BfaI CTAG 3 cut(s) 260, 342, 504
BfrI CTTAAG 1 cut(s) 89
BisI GCNGC 2 cut(s) 95, 401
BlsI GCNGC 2 cut(s) 96, 402
Bme1390I CCNGG 2 cut(s) 99, 441
BmgT120I GGNCC 2 cut(s) 215, 287
BmrFI CCNGG 2 cut(s) 99, 441
BmsI GCATC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 455, 487
Bpu14I TTCGAA 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 38
BseBI CCWGG 2 cut(s) 99, 441
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 38
BseXI GCAGC 1 cut(s) 387
BshFI GGCC 3 cut(s) 97, 217, 289
BslI CCNNNNNNNGG 1 cut(s) 305
BsnI GGCC 3 cut(s) 97, 217, 289
Bsp119I TTCGAA 1 cut(s) 173
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 406, 487
BspACI CCGC 2 cut(s) 94, 296
BspANI GGCC 3 cut(s) 97, 217, 289
BspCNI CTCAG 1 cut(s) 315
BspT104I TTCGAA 1 cut(s) 173
BspTI CTTAAG 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 2 cut(s) 406, 487
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 2 cut(s) 99, 441
Bst4CI ACNGT 3 cut(s) 72, 134, 386
BstAFI CTTAAG 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 167, 473
BstDEI CTNAG 2 cut(s) 232, 323
BstEII GGTNACC 1 cut(s) 101
BstF5I GGATG 1 cut(s) 243
BstKTI GATC 2 cut(s) 409, 490
BstMBI GATC 2 cut(s) 406, 487
BstMWI GCNNNNNNNGC 2 cut(s) 295, 472
BstNI CCWGG 2 cut(s) 99, 441
BstNSI RCATGY 1 cut(s) 10
BstPI GGTNACC 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 97, 439
BstV1I GCAGC 1 cut(s) 387
BsuRI GGCC 3 cut(s) 97, 217, 289
BtsCI GGATG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 45, 382
Cac8I GCNNGC 2 cut(s) 167, 473
Cfr13I GGNCC 2 cut(s) 215, 287
Csp6I GTAC 7 cut(s) 4, 110, 119, 127, 190, 257, 525
CviAII CATG 2 cut(s) 7, 533
CviQI GTAC 7 cut(s) 4, 110, 119, 127, 190, 257, 525
DdeI CTNAG 2 cut(s) 232, 323
DpnI GATC 2 cut(s) 408, 489
DpnII GATC 2 cut(s) 406, 487
EciI GGCGGA 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 250
Eco32I GATATC 1 cut(s) 329
Eco91I GGTNACC 1 cut(s) 101
EcoO65I GGTNACC 1 cut(s) 101
EcoRI GAATTC 1 cut(s) 33
EcoRII CCWGG 2 cut(s) 97, 439
EcoRV GATATC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 2 cut(s) 10, 536
FaiI YATR 4 cut(s) 8, 395, 513, 534
FatI CATG 2 cut(s) 6, 532
Fnu4HI GCNGC 2 cut(s) 95, 401
FokI GGATG 1 cut(s) 250
Fsp4HI GCNGC 2 cut(s) 95, 401
FspBI CTAG 3 cut(s) 260, 342, 504
GluI GCNGC 2 cut(s) 95, 401
HaeIII GGCC 3 cut(s) 97, 217, 289
Hin1II CATG 2 cut(s) 10, 536
HindIII AAGCTT 2 cut(s) 20, 167
HinfI GANTC 1 cut(s) 77
Hpy166II GTNNAC 2 cut(s) 121, 257
Hpy188I TCNGA 2 cut(s) 84, 358
Hpy188III TCNNGA 2 cut(s) 12, 342
Hpy8I GTNNAC 2 cut(s) 121, 257
HpyAV CCTTC 2 cut(s) 134, 309
HpyCH4III ACNGT 3 cut(s) 72, 134, 386
HpyCH4V TGCA 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 2 cut(s) 295, 472
HpyF3I CTNAG 2 cut(s) 232, 323
Hsp92II CATG 2 cut(s) 10, 536
Kzo9I GATC 2 cut(s) 406, 487
LmnI GCTCC 1 cut(s) 463
LpnPI CCDG 8 cut(s) 12, 51, 84, 111, 237, 350, 426, 453
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 1 cut(s) 387
MaeI CTAG 3 cut(s) 260, 342, 504
MaeIII GTNAC 2 cut(s) 40, 101
MalI GATC 2 cut(s) 408, 489
MboI GATC 2 cut(s) 406, 487
MboII GAAGA 1 cut(s) 147
MfeI CAATTG 1 cut(s) 369
MluCI AATT 9 cut(s) 33, 147, 175, 207, 263, 274, 291, 369, 493
MnlI CCTC 3 cut(s) 56, 177, 461
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 2 cut(s) 90, 477
MspA1I CMGCKG 2 cut(s) 64, 322
MspCI CTTAAG 1 cut(s) 89
MspR9I CCNGG 2 cut(s) 99, 441
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 2 cut(s) 99, 441
MwoI GCNNNNNNNGC 2 cut(s) 295, 472
NdeII GATC 2 cut(s) 406, 487
NlaIII CATG 2 cut(s) 10, 536
NmuCI GTSAC 1 cut(s) 40
NspI RCATGY 1 cut(s) 10
NspV TTCGAA 1 cut(s) 173
PciI ACATGT 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 77
PfoI TCCNGGA 1 cut(s) 439
PkrI GCNGC 2 cut(s) 96, 402
PscI ACATGT 1 cut(s) 6
Psp6I CCWGG 2 cut(s) 97, 439
PspEI GGTNACC 1 cut(s) 101
PspGI CCWGG 2 cut(s) 97, 439
PspPI GGNCC 2 cut(s) 215, 287
PvuII CAGCTG 2 cut(s) 64, 322
RsaI GTAC 7 cut(s) 5, 111, 120, 128, 191, 258, 526
RsaNI GTAC 7 cut(s) 4, 110, 119, 127, 190, 257, 525
SaqAI TTAA 2 cut(s) 90, 477
SatI GCNGC 2 cut(s) 95, 401
Sau3AI GATC 2 cut(s) 406, 487
Sau96I GGNCC 2 cut(s) 215, 287
ScrFI CCNGG 2 cut(s) 99, 441
SetI ASST 9 cut(s) 24, 66, 108, 171, 188, 252, 324, 339, 378
SfaNI GCATC 1 cut(s) 387
SfuI TTCGAA 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 9 cut(s) 33, 147, 175, 207, 263, 274, 291, 369, 493
SsiI CCGC 2 cut(s) 94, 296
SspMI CTAG 3 cut(s) 260, 342, 504
StyD4I CCNGG 2 cut(s) 97, 439
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 3 cut(s) 72, 134, 386
TaqI TCGA 2 cut(s) 173, 278
TasI AATT 9 cut(s) 33, 147, 175, 207, 263, 274, 291, 369, 493
TatI WGTACW 3 cut(s) 3, 109, 256
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 2 cut(s) 90, 477
Tru9I TTAA 2 cut(s) 90, 477
TscAI CASTG 2 cut(s) 45, 389
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 507
TspRI CASTG 2 cut(s) 45, 389
Vha464I CTTAAG 1 cut(s) 89
XapI RAATTY 4 cut(s) 33, 147, 175, 263
XbaI TCTAGA 1 cut(s) 341
XceI RCATGY 1 cut(s) 10
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 3 cut(s) 260, 342, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.