Rw5G049760

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004852
Physical Location & Seq
Forward (+)
86317501 .. 86321133
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049760.1

Sequence Viewer

Length: 834 bp
ATGTCCAGTAGCACCCAGAGGCAGAGGCGATTCTGTTACTTTTATATCACCGCGTCACAGTGCGAGCCAATTGACCAACCTTACTTGGGCAATGCTCTGATCAAAAATCTATTCTTCAAGCTAACTCGTCCCTTTCGTCCTTGGATTGACCCACGCGGTGCCAACATGATTCCCATCCATATAATGCTCTTCAACCAACCCGGAACCCGTCTCTCCATTTATTCTCCAATCGTGTTTGGTGAACCAAACCTCGTGACTGAAAGACGGTTTATGCTCCCTCTCTCAGATGTCATAACTGAAATTGGTGTGTATAAGGACATCATTGAGGGGTATCTTTCCACCATGGGAGTCCGACGAAATATGCACCCAGATGCTATTGGAAAAATACATGACGTGATTCAAGTGGCCAACCCCGGGTTTCCTATTGATGTGACAATATATGACGTGACTGTTGATGTATTGGGCAAGTCTGAAGTTGTTCCGCCGACTATATCAGTGTTTCACATTGTGGAACAAGTTGGACTTGATAGTTTGGAAGCTACTATCAGACAGAAGCCATGTGCTATTTGTCGTGAGAGCCTTGATCACGTTTATGACGCAGAAGAAGGGATTATTCGCTTGCCCTGCTTGCACCTTTTTCATGCAGATTGCCTTATCCAGTGTTTGAATGATCATGTGCATGTTTGCCCCCTCTGCAATTACCCACTGCCTACTGTGGAACTTGGTGAACCATCCAAACCCTCGGGGCAACTGGATTGGCGTGTGTTGCTCGTGATGTCGGCATGTGGTATAATAACTGTTAAGCTTTTTTGCAGACTATTAAAGCGACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

277

Amino Acids

31.36

Weight (kDa)

6.49

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 187 - 233 2.5e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 2 cut(s) 53, 156
AciI CCGC 3 cut(s) 51, 156, 482
AcoI YGGCCR 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 492
AdeI CACNNNGTG 2 cut(s) 158, 508
AfiI CCNNNNNNNGG 2 cut(s) 86, 414
AgsI TTSAA 4 cut(s) 118, 193, 401, 667
AjiI CACGTC 2 cut(s) 394, 445
AluBI AGCT 3 cut(s) 121, 539, 805
AluI AGCT 3 cut(s) 121, 539, 805
Alw26I GTCTC 1 cut(s) 215
Ama87I CYCGRG 2 cut(s) 413, 742
AoxI GGCC 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 477
AsuC2I CCSGG 3 cut(s) 201, 414, 415
AsuHPI GGTGA 3 cut(s) 40, 251, 737
AvaI CYCGRG 2 cut(s) 413, 742
BalI TGGCCA 1 cut(s) 407
BanI GGYRCC 1 cut(s) 158
BauI CACGAG 2 cut(s) 251, 770
BccI CCATC 2 cut(s) 182, 739
BclI TGATCA 3 cut(s) 99, 583, 670
BcnI CCSGG 3 cut(s) 201, 414, 415
BcoDI GTCTC 1 cut(s) 215
Bme1390I CCNGG 3 cut(s) 201, 414, 415
BmeT110I CYCGRG 2 cut(s) 413, 742
BmgBI CACGTC 2 cut(s) 394, 445
BmiI GGNNCC 2 cut(s) 160, 205
BmrFI CCNGG 3 cut(s) 201, 414, 415
BmsI GCATC 1 cut(s) 361
BpuMI CCSGG 3 cut(s) 201, 414, 415
BsaBI GATNNNNATC 1 cut(s) 173
BsaJI CCNNGG 5 cut(s) 140, 342, 412, 413, 741
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 414
Bse1I ACTGG 3 cut(s) 6, 658, 756
Bse3DI GCAATG 1 cut(s) 97
Bse8I GATNNNNATC 1 cut(s) 173
BseDI CCNNGG 5 cut(s) 140, 342, 412, 413, 741
BseGI GGATG 2 cut(s) 174, 731
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 2 cut(s) 86, 414
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 3 cut(s) 6, 658, 756
Bsh1236I CGCG 2 cut(s) 53, 156
BshFI GGCC 1 cut(s) 407
BshNI GGYRCC 1 cut(s) 158
BsiHKCI CYCGRG 2 cut(s) 413, 742
BsiSI CCGG 2 cut(s) 201, 414
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 2 cut(s) 86, 414
BsmAI GTCTC 1 cut(s) 215
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 407
BsoBI CYCGRG 2 cut(s) 413, 742
Bsp143I GATC 3 cut(s) 99, 583, 670
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 3 cut(s) 51, 156, 482
BspANI GGCC 1 cut(s) 407
BspCNI CTCAG 1 cut(s) 296
BspFNI CGCG 2 cut(s) 53, 156
BspLI GGNNCC 2 cut(s) 160, 205
BspQI GCTCTTC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 3 cut(s) 6, 658, 756
BssECI CCNNGG 5 cut(s) 140, 342, 412, 413, 741
BssMI GATC 3 cut(s) 99, 583, 670
BssSI CACGAG 2 cut(s) 251, 770
BssT1I CCWWGG 2 cut(s) 140, 342
Bst2BI CACGAG 2 cut(s) 251, 770
Bst4CI ACNGT 5 cut(s) 60, 267, 451, 715, 799
Bst6I CTCTTC 1 cut(s) 194
BstC8I GCNNGC 3 cut(s) 65, 620, 629
BstDEI CTNAG 1 cut(s) 283
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 2 cut(s) 174, 731
BstFNI CGCG 2 cut(s) 53, 156
BstKTI GATC 3 cut(s) 102, 586, 673
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 3 cut(s) 99, 583, 670
BstMWI GCNNNNNNNGC 4 cut(s) 624, 628, 693, 766
BstNSI RCATGY 2 cut(s) 683, 786
BstSCI CCNGG 3 cut(s) 199, 412, 413
BstUI CGCG 2 cut(s) 53, 156
BsuRI GGCC 1 cut(s) 407
BtgI CCRYGG 1 cut(s) 342
BtrI CACGTC 2 cut(s) 394, 445
BtsCI GGATG 2 cut(s) 174, 731
BtsI GCAGTG 1 cut(s) 704
BtsIMutI CAGTG 4 cut(s) 65, 501, 665, 704
Cac8I GCNNGC 3 cut(s) 65, 620, 629
Cfr9I CCCGGG 1 cut(s) 413
CseI GACGC 2 cut(s) 42, 605
CviAII CATG 8 cut(s) 166, 343, 389, 558, 641, 674, 680, 783
CviJI RGCY 7 cut(s) 67, 121, 407, 539, 556, 579, 805
CviKI_1 RGCY 7 cut(s) 67, 121, 407, 539, 556, 579, 805
DdeI CTNAG 1 cut(s) 283
DpnI GATC 3 cut(s) 101, 585, 672
DpnII GATC 3 cut(s) 99, 583, 670
DraIII CACNNNGTG 2 cut(s) 158, 508
EaeI YGGCCR 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 1 cut(s) 471
Eco130I CCWWGG 2 cut(s) 140, 342
Eco57I CTGAAG 1 cut(s) 492
Eco88I CYCGRG 2 cut(s) 413, 742
EcoT14I CCWWGG 2 cut(s) 140, 342
ErhI CCWWGG 2 cut(s) 140, 342
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 8 cut(s) 169, 346, 392, 561, 644, 677, 683, 786
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 1 cut(s) 114
FatI CATG 8 cut(s) 165, 342, 388, 557, 640, 673, 679, 782
FbaI TGATCA 3 cut(s) 99, 583, 670
FokI GGATG 2 cut(s) 161, 718
HaeIII GGCC 1 cut(s) 407
HapII CCGG 2 cut(s) 201, 414
HgaI GACGC 2 cut(s) 42, 605
Hin1II CATG 8 cut(s) 169, 346, 392, 561, 644, 677, 683, 786
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 4 cut(s) 30, 169, 348, 397
HpaII CCGG 2 cut(s) 201, 414
HphI GGTGA 3 cut(s) 40, 251, 737
Hpy166II GTNNAC 2 cut(s) 242, 728
Hpy188I TCNGA 5 cut(s) 99, 286, 353, 472, 548
Hpy188III TCNNGA 3 cut(s) 253, 572, 772
Hpy8I GTNNAC 2 cut(s) 242, 728
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 1 cut(s) 599
HpyCH4III ACNGT 5 cut(s) 60, 267, 451, 715, 799
HpyCH4IV ACGT 3 cut(s) 393, 444, 588
HpyCH4V TGCA 6 cut(s) 364, 631, 644, 679, 696, 813
HpyF10VI GCNNNNNNNGC 4 cut(s) 624, 628, 693, 766
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 3 cut(s) 393, 444, 588
Hsp92II CATG 8 cut(s) 169, 346, 392, 561, 644, 677, 683, 786
Ksp22I TGATCA 3 cut(s) 99, 583, 670
Kzo9I GATC 3 cut(s) 99, 583, 670
LguI GCTCTTC 1 cut(s) 194
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 8 cut(s) 19, 29, 214, 381, 427, 637, 671, 737
LweI GCATC 1 cut(s) 361
MaeII ACGT 3 cut(s) 393, 444, 588
MaeIII GTNAC 5 cut(s) 35, 54, 253, 430, 445
MalI GATC 3 cut(s) 101, 585, 672
MboI GATC 3 cut(s) 99, 583, 670
MboII GAAGA 3 cut(s) 106, 181, 614
MfeI CAATTG 1 cut(s) 69
MlsI TGGCCA 1 cut(s) 407
MluCI AATT 3 cut(s) 69, 300, 697
MluNI TGGCCA 1 cut(s) 407
MlyI GAGTC 1 cut(s) 357
MmeI TCCRAC 2 cut(s) 376, 499
MnlI CCTC 7 cut(s) 12, 18, 260, 288, 319, 701, 751
Mox20I TGGCCA 1 cut(s) 407
MroXI GAANNNNTTC 1 cut(s) 477
MscI TGGCCA 1 cut(s) 407
MseI TTAA 2 cut(s) 801, 821
MslI CAYNNNNRTG 3 cut(s) 369, 591, 678
Msp20I TGGCCA 1 cut(s) 407
MspI CCGG 2 cut(s) 201, 414
MspR9I CCNGG 3 cut(s) 201, 414, 415
MunI CAATTG 1 cut(s) 69
MvnI CGCG 2 cut(s) 53, 156
MwoI GCNNNNNNNGC 4 cut(s) 624, 628, 693, 766
NciI CCSGG 3 cut(s) 201, 414, 415
NcoI CCATGG 1 cut(s) 342
NdeII GATC 3 cut(s) 99, 583, 670
NlaIII CATG 8 cut(s) 169, 346, 392, 561, 644, 677, 683, 786
NlaIV GGNNCC 2 cut(s) 160, 205
NmuCI GTSAC 4 cut(s) 54, 253, 430, 445
NspI RCATGY 2 cut(s) 683, 786
PciSI GCTCTTC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 133
PdmI GAANNNNTTC 1 cut(s) 477
PfeI GAWTC 3 cut(s) 30, 169, 397
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
PspN4I GGNNCC 2 cut(s) 160, 205
RseI CAYNNNNRTG 3 cut(s) 369, 591, 678
SapI GCTCTTC 1 cut(s) 194
SaqAI TTAA 2 cut(s) 801, 821
Sau3AI GATC 3 cut(s) 99, 583, 670
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 3 cut(s) 201, 414, 415
SetI ASST 9 cut(s) 82, 123, 252, 396, 447, 541, 591, 636, 807
SfaNI GCATC 1 cut(s) 361
SmaI CCCGGG 1 cut(s) 415
SmiMI CAYNNNNRTG 3 cut(s) 369, 591, 678
Sse9I AATT 3 cut(s) 69, 300, 697
SsiI CCGC 3 cut(s) 51, 156, 482
StyD4I CCNGG 3 cut(s) 199, 412, 413
StyI CCWWGG 2 cut(s) 140, 342
TaaI ACNGT 5 cut(s) 60, 267, 451, 715, 799
TaiI ACGT 3 cut(s) 396, 447, 591
TasI AATT 3 cut(s) 69, 300, 697
TfiI GAWTC 3 cut(s) 30, 169, 397
Tru1I TTAA 2 cut(s) 801, 821
Tru9I TTAA 2 cut(s) 801, 821
TscAI CASTG 4 cut(s) 65, 501, 665, 711
TseFI GTSAC 4 cut(s) 54, 253, 430, 445
Tsp45I GTSAC 4 cut(s) 54, 253, 430, 445
TspDTI ATGAA 1 cut(s) 629
TspMI CCCGGG 1 cut(s) 413
TspRI CASTG 4 cut(s) 65, 501, 665, 711
XceI RCATGY 2 cut(s) 683, 786
XmaI CCCGGG 1 cut(s) 413
XmnI GAANNNNTTC 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.