FvH4_4g01770
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. MPBQ MBSQ MT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002098
Physical Location & Seq
Reverse (-)
1550829 .. 1554369
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01770.t1

Sequence Viewer

Length: 1020 bp
ATGGCTATTTCAATGCTCTATGGAGCTCAGAATCTGAAACTTGCTTCTGGCATAGCACCAACTGGGTTAGGTTCTGGTGGGAATTTTCAATATGGATCTTTCCCCAAAACGGGTTCTGTTAGTTATGGTAGGAATTCCAAGGCTAGAACTATTGGACCCATATGCAGTGTCTCGTCCTCAAGGCCTGCTTCTCAGCCTAGGTTCATACAGCACAAGCAAGAAGCATTCTGGTTCTATAGGTTCTTATCTATAGTCTATGATCATGTGATAAACCCTGGGCATTGGACTGAGGATATGAGAGACGACGCCCTTGAGCCGGCTGATCTTTATGACAGGAACATGACAGTGGTGGATGTTGGAGGTGGAACTGGGTTCACAACACTGGGGATTGTGAAGCATGTGGATGCCAAAAATGTTACAATTCTTGACCAATCACCACACCAGCTTGCCAAGGCTAAGGAGAAGGAGCCCTTGAAAGATTGCAAGATAATTGAAGGTGATGCCGAGGATCTGCCGTTCCCAACTGATTATGCAGATAGATATGTCTCTGCTGGAAGTATAGAGTATTGGCCGGATCCCCAGTGTGGAATCAAGGAAGCATACAGGGTACTTAAAATAGGAGGAAAAGCCTGTCTTATAGGCCCTGTGTACCCGACCTTCTGGCTGTCTCGTTTTTTCGCAGACACGTGGATGCTCTTCCCAACAGAGGAAGAATACATTGATTGGTTCAAAAAGGCAGGTTTCAAAGATGTTAAACTAAAAAGAATTGGTCCAAAGTGGTACCGTGGAGTTCGCCGGCATGGCCTGATTATGGGTTGCTCTGTGACAGGTGTGAAGCCCTTAACTGGGGATTCCCCTCTTCAGCTTGGTCCAAAGGTTGAGGATGTGAGGAAGCCTGTCAATCGATTTGTGTTTCTTTTCCGGCTCATTCTTGGAGCAACTGCAGGAGCTTACTATGTGCTCGTTCCCATTTACATGTGGATCAAAGATCAAATCGTTCCAAAGGGAAAACCTATCTGA

Protein Analysis

340

Amino Acids

37.9

Weight (kDa)

9.14

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 111 - 214 7e-13 Methyltransferase domain
Ubie_methyltran PF01209 112 - 212 7.6e-12 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 116 - 208 1.6e-16 Methyltransferase domain
Methyltransf_11 PF08241 117 - 211 2.2e-18 Methyltransferase domain
Methyltransf_12 PF08242 117 - 209 2.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 728
Acc65I GGTACC 1 cut(s) 780
AccB1I GGYRCC 1 cut(s) 780
AclWI GGATC 5 cut(s) 103, 516, 569, 582, 989
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 2 cut(s) 82, 133
AcuI CTGAAG 1 cut(s) 845
AcvI CACGTG 1 cut(s) 687
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 3 cut(s) 609, 650, 782
AfiI CCNNNNNNNGG 8 cut(s) 109, 110, 316, 584, 706, 811, 845, 846
AflIII ACRYGT 2 cut(s) 684, 975
AgsI TTSAA 6 cut(s) 12, 89, 475, 494, 730, 745
AjnI CCWGG 1 cut(s) 274
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 4 cut(s) 26, 445, 865, 950
AluI AGCT 4 cut(s) 26, 445, 865, 950
Alw21I GWGCWC 2 cut(s) 28, 963
Alw26I GTCTC 4 cut(s) 175, 294, 550, 672
AlwI GGATC 5 cut(s) 103, 516, 569, 582, 989
AlwNI CAGNNNCTG 1 cut(s) 34
AoxI GGCC 4 cut(s) 182, 569, 640, 802
ApoI RAATTY 2 cut(s) 82, 133
Asp718I GGTACC 1 cut(s) 780
AspA2I CCTAGG 1 cut(s) 197
AspS9I GGNCC 4 cut(s) 155, 641, 770, 869
AsuHPI GGTGA 2 cut(s) 426, 509
AvaII GGWCC 3 cut(s) 155, 770, 869
AvrII CCTAGG 1 cut(s) 197
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 780
BanII GRGCYC 2 cut(s) 28, 471
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbrPI CACGTG 1 cut(s) 687
Bbv12I GWGCWC 2 cut(s) 28, 963
BceAI ACGGC 1 cut(s) 499
BciT130I CCWGG 1 cut(s) 276
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 4 cut(s) 175, 294, 550, 672
BfaI CTAG 2 cut(s) 144, 198
BfmI CTRYAG 3 cut(s) 235, 249, 942
BfuAI ACCTGC 1 cut(s) 728
BglI GCCNNNNNGGC 1 cut(s) 801
BlnI CCTAGG 1 cut(s) 197
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 3 cut(s) 155, 770, 869
BmgT120I GGNCC 4 cut(s) 155, 641, 770, 869
BmiI GGNNCC 4 cut(s) 157, 468, 576, 782
BmrFI CCNGG 1 cut(s) 276
BmrI ACTGGG 5 cut(s) 72, 378, 392, 574, 855
BmsI GCATC 3 cut(s) 394, 490, 681
BmuI ACTGGG 5 cut(s) 72, 378, 392, 574, 855
Bpu10I CCTNAGC 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 163, 332
Bsa29I ATCGAT 1 cut(s) 904
BsaAI YACGTR 1 cut(s) 687
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 7 cut(s) 138, 197, 274, 275, 450, 504, 784
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 110, 316, 584, 706, 811, 845, 846
Bse118I RCCGGY 2 cut(s) 316, 795
Bse1I ACTGG 5 cut(s) 67, 373, 387, 580, 850
BseBI CCWGG 1 cut(s) 276
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 7 cut(s) 138, 197, 274, 275, 450, 504, 784
BseGI GGATG 4 cut(s) 358, 409, 696, 889
BseLI CCNNNNNNNGG 8 cut(s) 109, 110, 316, 584, 706, 811, 845, 846
BseMII CTCAG 3 cut(s) 41, 206, 279
BseNI ACTGG 5 cut(s) 67, 373, 387, 580, 850
BshFI GGCC 4 cut(s) 184, 571, 642, 804
BshNI GGYRCC 1 cut(s) 780
BshVI ATCGAT 1 cut(s) 904
BsiHKAI GWGCWC 2 cut(s) 28, 963
BsiSI CCGG 4 cut(s) 317, 572, 796, 922
BslI CCNNNNNNNGG 8 cut(s) 109, 110, 316, 584, 706, 811, 845, 846
BsmAI GTCTC 4 cut(s) 175, 294, 550, 672
BsmBI CGTCTC 1 cut(s) 294
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 4 cut(s) 184, 571, 642, 804
Bsp1286I GDGCHC 3 cut(s) 28, 471, 963
Bsp143I GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
BspANI GGCC 4 cut(s) 184, 571, 642, 804
BspCNI CTCAG 3 cut(s) 40, 205, 280
BspDI ATCGAT 1 cut(s) 904
BspLI GGNNCC 4 cut(s) 157, 468, 576, 782
BspMAI CTGCAG 1 cut(s) 946
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 5 cut(s) 103, 516, 569, 582, 989
BspQI GCTCTTC 1 cut(s) 701
BspT107I GGYRCC 1 cut(s) 780
BsrFI RCCGGY 2 cut(s) 316, 795
BsrI ACTGG 5 cut(s) 67, 373, 387, 580, 850
BssAI RCCGGY 2 cut(s) 316, 795
BssECI CCNNGG 7 cut(s) 138, 197, 274, 275, 450, 504, 784
BssMI GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 3 cut(s) 138, 197, 450
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 346, 785
Bst6I CTCTTC 2 cut(s) 701, 864
BstACI GRCGYC 1 cut(s) 306
BstBAI YACGTR 1 cut(s) 687
BstC8I GCNNGC 4 cut(s) 186, 318, 447, 797
BstDEI CTNAG 4 cut(s) 27, 192, 288, 456
BstDSI CCRYGG 1 cut(s) 784
BstF5I GGATG 4 cut(s) 358, 409, 696, 889
BstKTI GATC 7 cut(s) 98, 262, 325, 511, 577, 984, 991
BstMAI GTCTC 4 cut(s) 175, 294, 550, 672
BstMBI GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
BstMWI GCNNNNNNNGC 1 cut(s) 801
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 2 cut(s) 401, 979
BstSCI CCNGG 1 cut(s) 274
BstSFI CTRYAG 3 cut(s) 235, 249, 942
BstX2I RGATCY 3 cut(s) 95, 508, 574
BstYI RGATCY 3 cut(s) 95, 508, 574
Bsu15I ATCGAT 1 cut(s) 904
BsuRI GGCC 4 cut(s) 184, 571, 642, 804
BsuTUI ATCGAT 1 cut(s) 904
BtgI CCRYGG 1 cut(s) 784
BtsCI GGATG 4 cut(s) 358, 409, 696, 889
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 4 cut(s) 172, 351, 380, 587
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 4 cut(s) 186, 318, 447, 797
CaiI CAGNNNCTG 1 cut(s) 34
Cfr10I RCCGGY 2 cut(s) 316, 795
Cfr13I GGNCC 4 cut(s) 155, 641, 770, 869
ClaI ATCGAT 1 cut(s) 904
CseI GACGC 1 cut(s) 314
Csp6I GTAC 3 cut(s) 608, 649, 781
CviAII CATG 5 cut(s) 263, 340, 398, 800, 976
CviQI GTAC 3 cut(s) 608, 649, 781
DdeI CTNAG 4 cut(s) 27, 192, 288, 456
DpnI GATC 7 cut(s) 97, 261, 324, 510, 576, 983, 990
DpnII GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
EaeI YGGCCR 1 cut(s) 569
Eam1104I CTCTTC 2 cut(s) 701, 864
EarI CTCTTC 2 cut(s) 701, 864
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 3 cut(s) 138, 197, 450
Eco147I AGGCCT 1 cut(s) 184
Eco24I GRGCYC 2 cut(s) 28, 471
Eco47I GGWCC 3 cut(s) 155, 770, 869
Eco53kI GAGCTC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 845
Eco72I CACGTG 1 cut(s) 687
EcoICRI GAGCTC 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 641
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 3 cut(s) 138, 197, 450
EcoT38I GRGCYC 2 cut(s) 28, 471
ErhI CCWWGG 3 cut(s) 138, 197, 450
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 5 cut(s) 266, 343, 401, 803, 979
FalI AAGNNNNNCTT 6 cut(s) 172, 204, 455, 487, 618, 650
FatI CATG 5 cut(s) 262, 339, 397, 799, 975
FauNDI CATATG 1 cut(s) 161
FbaI TGATCA 1 cut(s) 259
FokI GGATG 4 cut(s) 365, 416, 703, 896
FriOI GRGCYC 2 cut(s) 28, 471
FspBI CTAG 2 cut(s) 144, 198
HaeIII GGCC 4 cut(s) 184, 571, 642, 804
HapII CCGG 4 cut(s) 317, 572, 796, 922
HgaI GACGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 5 cut(s) 266, 343, 401, 803, 979
HinfI GANTC 3 cut(s) 31, 588, 851
HpaII CCGG 4 cut(s) 317, 572, 796, 922
HphI GGTGA 2 cut(s) 426, 509
Hpy166II GTNNAC 2 cut(s) 375, 649
Hpy188I TCNGA 3 cut(s) 30, 36, 1019
Hpy188III TCNNGA 1 cut(s) 425
Hpy8I GTNNAC 2 cut(s) 375, 649
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 3 cut(s) 457, 488, 667
HpyCH4III ACNGT 2 cut(s) 346, 785
HpyCH4IV ACGT 1 cut(s) 686
HpyCH4V TGCA 4 cut(s) 165, 483, 533, 944
HpyF10VI GCNNNNNNNGC 1 cut(s) 801
HpyF3I CTNAG 4 cut(s) 27, 192, 288, 456
HpySE526I ACGT 1 cut(s) 686
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 5 cut(s) 266, 343, 401, 803, 979
KpnI GGTACC 1 cut(s) 784
KroI GCCGGC 2 cut(s) 316, 795
KroNI GCCGGC 2 cut(s) 318, 797
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
LguI GCTCTTC 1 cut(s) 701
LmnI GCTCC 4 cut(s) 23, 466, 935, 947
LweI GCATC 3 cut(s) 394, 490, 681
MaeI CTAG 2 cut(s) 144, 198
MaeII ACGT 1 cut(s) 686
MaeIII GTNAC 2 cut(s) 415, 823
MalI GATC 7 cut(s) 97, 261, 324, 510, 576, 983, 990
MboI GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
MboII GAAGA 3 cut(s) 688, 722, 851
MflI RGATCY 3 cut(s) 95, 508, 574
MhlI GDGCHC 3 cut(s) 28, 471, 963
MluCI AATT 5 cut(s) 82, 133, 420, 489, 765
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 9 cut(s) 187, 283, 353, 499, 614, 700, 867, 874, 882
MroNI GCCGGC 2 cut(s) 316, 795
MseI TTAA 3 cut(s) 612, 753, 842
MslI CAYNNNNRTG 4 cut(s) 344, 402, 689, 974
MspI CCGG 4 cut(s) 317, 572, 796, 922
MspR9I CCNGG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 801
NaeI GCCGGC 2 cut(s) 318, 797
NdeI CATATG 1 cut(s) 161
NdeII GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
NgoMIV GCCGGC 2 cut(s) 316, 795
NlaIII CATG 5 cut(s) 266, 343, 401, 803, 979
NlaIV GGNNCC 4 cut(s) 157, 468, 576, 782
NmeAIII GCCGAG 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 823
NspI RCATGY 2 cut(s) 401, 979
PasI CCCWGGG 1 cut(s) 275
PceI AGGCCT 1 cut(s) 184
PciI ACATGT 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 701
PctI GAATGC 1 cut(s) 224
PdiI GCCGGC 2 cut(s) 318, 797
PfeI GAWTC 3 cut(s) 31, 588, 851
PmaCI CACGTG 1 cut(s) 687
PmlI CACGTG 1 cut(s) 687
Ppu21I YACGTR 1 cut(s) 687
PscI ACATGT 1 cut(s) 975
Psp124BI GAGCTC 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 274
PspCI CACGTG 1 cut(s) 687
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 4 cut(s) 157, 468, 576, 782
PspPI GGNCC 4 cut(s) 155, 641, 770, 869
PstI CTGCAG 1 cut(s) 946
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 3 cut(s) 95, 508, 574
RsaI GTAC 3 cut(s) 609, 650, 782
RsaNI GTAC 3 cut(s) 608, 649, 781
RseI CAYNNNNRTG 4 cut(s) 344, 402, 689, 974
SacI GAGCTC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 701
SaqAI TTAA 3 cut(s) 612, 753, 842
Sau3AI GATC 7 cut(s) 95, 259, 322, 508, 574, 981, 988
Sau96I GGNCC 4 cut(s) 155, 641, 770, 869
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 3 cut(s) 28, 471, 963
SfaNI GCATC 3 cut(s) 394, 490, 681
SfcI CTRYAG 3 cut(s) 235, 249, 942
SinI GGWCC 3 cut(s) 155, 770, 869
SmiMI CAYNNNNRTG 4 cut(s) 344, 402, 689, 974
SmlI CTYRAG 2 cut(s) 178, 311
SmoI CTYRAG 2 cut(s) 178, 311
Sse9I AATT 5 cut(s) 82, 133, 420, 489, 765
SseBI AGGCCT 1 cut(s) 184
SspMI CTAG 2 cut(s) 144, 198
SstI GAGCTC 1 cut(s) 28
StuI AGGCCT 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 3 cut(s) 138, 197, 450
TaaI ACNGT 2 cut(s) 346, 785
TaiI ACGT 1 cut(s) 689
TaqI TCGA 1 cut(s) 904
TasI AATT 5 cut(s) 82, 133, 420, 489, 765
TfiI GAWTC 3 cut(s) 31, 588, 851
Tru1I TTAA 3 cut(s) 612, 753, 842
Tru9I TTAA 3 cut(s) 612, 753, 842
TscAI CASTG 4 cut(s) 172, 351, 387, 587
TseFI GTSAC 1 cut(s) 823
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 1 cut(s) 193
TspRI CASTG 4 cut(s) 172, 351, 387, 587
VpaK11BI GGWCC 3 cut(s) 155, 770, 869
XapI RAATTY 2 cut(s) 82, 133
XceI RCATGY 2 cut(s) 401, 979
XmaJI CCTAGG 1 cut(s) 197
XspI CTAG 2 cut(s) 144, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.