Rh4DG018100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. MPBQ MBSQ MT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002098
Physical Location & Seq
Reverse (-)
2854786 .. 2857083
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG018100.1

Sequence Viewer

Length: 1020 bp
ATGGCTATTTCAATGCTCTATGGAGCTCAAAATCTGAAACTCACTTCTGGCATAGCACCGACTTGGTTAGGTTCTGGGGGAAATTTTCAATATGGGTCTTTCCCCAAAAAGGTTTCAGTTAGTTATGGAAGGAATTCCAAGGCTAGAACTTTGGGACCCATATGCAGTGTCTCATCCTCAAGGCCTGCATCTCAGCCTAGGTTTATACAGCACAAGCAAGAAGCATTTTGGTTCTATAGGTTTTTATCTATAGTCTATGATCATGTGATAAACCCTGGGCATTGGACTGAGGATATGAGAGACGATGCTCTTGAGCCGGCTGATCTTTATGACAGGAATATGACAGTGGTAGATGTTGGAGGTGGTACTGGGTTTACAACACTGGGGATTGTGAAGCATGTGGATGCCAAAAATGTTACTATTCTTGACCAATCACCGCACCAGCTTGCCAAGGCTAAGCAGAAGGAGCCCTTGAAAGAATGCAAGATAATAGAAGGCGATGCCGAGGATCTGCCATTCCCAACTGATTATGCGGATAGATATGTCTCTGCTGGAAGTATAGAGTACTGGCCGGATCCACAGCGTGGAATCAAGGAAGCATACAGGGTACTAAAAATAGGAGGAAAAGCCTGTCTTATAGGCCCTGTGTACCCAACCTTCTGGCTGTCTCGTTTTTTCGCAGACGTGTGGATGCTCTTCCCAACAGAGGAAGAATACATTGAGTGGTTCCAAAAGGCAGGTTTCAAAGATGTTAAACTAAAAAGAATTGGTCCAAAGTGGTACCGTGGAGTTCGCCGGCATGGCCTGATTATGGGTTGCTCTGTGACAGGTGTGAAGCCCTTAACTGGGGATTCCCCTCTCCAGCTTGGTCCAAAGGTTGAGGATGTGAGGAAGCCCGTCAATCCATTTGTGTTTCTTTTCCGGCTAATTCTTGGAGCAACTGCAGGAGCTTACTATGTGCTGGTTCCCATTTACATGTGGATCAAAGATCAAATTGTTCCAAAGGGCAAACCTATCTGA

Protein Analysis

339

Amino Acids

38.15

Weight (kDa)

9.26

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 110 - 252 5.6e-06 Methyltransferase domain
Methyltransf_31 PF13847 111 - 215 1.3e-12 Methyltransferase domain
Ubie_methyltran PF01209 112 - 213 2.3e-12 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 116 - 208 3.8e-17 Methyltransferase domain
Methyltransf_11 PF08241 117 - 211 1.1e-19 Methyltransferase domain
Methyltransf_12 PF08242 117 - 209 1.8e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 728
Acc65I GGTACC 1 cut(s) 780
AccB1I GGYRCC 1 cut(s) 780
AccB7I CCANNNNNTGG 1 cut(s) 584
AciI CCGC 2 cut(s) 437, 533
AclWI GGATC 4 cut(s) 516, 569, 582, 989
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 2 cut(s) 82, 133
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 5 cut(s) 367, 566, 609, 650, 782
AfiI CCNNNNNNNGG 6 cut(s) 109, 584, 706, 811, 845, 846
AflIII ACRYGT 2 cut(s) 684, 975
AgsI TTSAA 4 cut(s) 12, 89, 475, 745
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 4 cut(s) 26, 445, 865, 950
AluI AGCT 4 cut(s) 26, 445, 865, 950
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 4 cut(s) 175, 294, 550, 672
AlwI GGATC 4 cut(s) 516, 569, 582, 989
AoxI GGCC 4 cut(s) 182, 569, 640, 802
ApoI RAATTY 2 cut(s) 82, 133
Asp700I GAANNNNTTC 1 cut(s) 133
Asp718I GGTACC 1 cut(s) 780
AspA2I CCTAGG 1 cut(s) 197
AspS9I GGNCC 4 cut(s) 155, 641, 770, 869
AsuHPI GGTGA 1 cut(s) 426
AvaII GGWCC 3 cut(s) 155, 770, 869
AvrII CCTAGG 1 cut(s) 197
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 780
BanII GRGCYC 2 cut(s) 28, 471
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
Bbv12I GWGCWC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 276
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 4 cut(s) 175, 294, 550, 672
BfaI CTAG 2 cut(s) 144, 198
BfmI CTRYAG 3 cut(s) 235, 249, 942
BfuAI ACCTGC 1 cut(s) 728
BglI GCCNNNNNGGC 1 cut(s) 801
BlnI CCTAGG 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 456
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 3 cut(s) 155, 770, 869
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 4 cut(s) 155, 641, 770, 869
BmiI GGNNCC 7 cut(s) 156, 157, 468, 576, 728, 782, 966
BmrFI CCNGG 1 cut(s) 276
BmrI ACTGGG 3 cut(s) 378, 392, 855
BmsI GCATC 5 cut(s) 197, 295, 394, 490, 681
BmuI ACTGGG 3 cut(s) 378, 392, 855
BpmI CTGGAG 1 cut(s) 845
Bpu1102I GCTNAGC 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 163, 332
BsaJI CCNNGG 7 cut(s) 138, 197, 274, 275, 450, 504, 784
Bsc4I CCNNNNNNNGG 6 cut(s) 109, 584, 706, 811, 845, 846
Bse118I RCCGGY 2 cut(s) 316, 795
Bse1I ACTGG 4 cut(s) 373, 387, 572, 850
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 7 cut(s) 138, 197, 274, 275, 450, 504, 784
BseGI GGATG 4 cut(s) 173, 409, 696, 889
BseLI CCNNNNNNNGG 6 cut(s) 109, 584, 706, 811, 845, 846
BseMII CTCAG 2 cut(s) 206, 279
BseNI ACTGG 4 cut(s) 373, 387, 572, 850
BshFI GGCC 4 cut(s) 184, 571, 642, 804
BshNI GGYRCC 1 cut(s) 780
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 4 cut(s) 317, 572, 796, 922
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 6 cut(s) 109, 584, 706, 811, 845, 846
BsmAI GTCTC 4 cut(s) 175, 294, 550, 672
BsmBI CGTCTC 1 cut(s) 294
BsmFI GGGAC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 4 cut(s) 184, 571, 642, 804
Bsp1286I GDGCHC 2 cut(s) 28, 471
Bsp143I GATC 6 cut(s) 259, 322, 508, 574, 981, 988
Bsp1720I GCTNAGC 1 cut(s) 456
BspACI CCGC 2 cut(s) 437, 533
BspANI GGCC 4 cut(s) 184, 571, 642, 804
BspCNI CTCAG 2 cut(s) 205, 280
BspLI GGNNCC 7 cut(s) 156, 157, 468, 576, 728, 782, 966
BspMAI CTGCAG 1 cut(s) 946
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 4 cut(s) 516, 569, 582, 989
BspQI GCTCTTC 1 cut(s) 701
BspT107I GGYRCC 1 cut(s) 780
BsrFI RCCGGY 2 cut(s) 316, 795
BsrI ACTGG 4 cut(s) 373, 387, 572, 850
BssAI RCCGGY 2 cut(s) 316, 795
BssECI CCNNGG 7 cut(s) 138, 197, 274, 275, 450, 504, 784
BssMI GATC 6 cut(s) 259, 322, 508, 574, 981, 988
BssT1I CCWWGG 3 cut(s) 138, 197, 450
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 346, 785
Bst6I CTCTTC 1 cut(s) 701
BstC8I GCNNGC 4 cut(s) 186, 318, 447, 797
BstDEI CTNAG 3 cut(s) 192, 288, 456
BstDSI CCRYGG 1 cut(s) 784
BstF5I GGATG 4 cut(s) 173, 409, 696, 889
BstKTI GATC 6 cut(s) 262, 325, 511, 577, 984, 991
BstMAI GTCTC 4 cut(s) 175, 294, 550, 672
BstMBI GATC 6 cut(s) 259, 322, 508, 574, 981, 988
BstMWI GCNNNNNNNGC 2 cut(s) 466, 801
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 2 cut(s) 401, 979
BstSCI CCNGG 1 cut(s) 274
BstSFI CTRYAG 3 cut(s) 235, 249, 942
BstX2I RGATCY 2 cut(s) 508, 574
BstXI CCANNNNNNTGG 1 cut(s) 660
BstYI RGATCY 2 cut(s) 508, 574
BsuRI GGCC 4 cut(s) 184, 571, 642, 804
BtgI CCRYGG 1 cut(s) 784
BtgZI GCGATG 1 cut(s) 513
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 4 cut(s) 173, 409, 696, 889
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 3 cut(s) 172, 351, 380
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 4 cut(s) 186, 318, 447, 797
Cfr10I RCCGGY 2 cut(s) 316, 795
Cfr13I GGNCC 4 cut(s) 155, 641, 770, 869
Csp6I GTAC 5 cut(s) 366, 565, 608, 649, 781
CviAII CATG 4 cut(s) 263, 398, 800, 976
CviQI GTAC 5 cut(s) 366, 565, 608, 649, 781
DdeI CTNAG 3 cut(s) 192, 288, 456
DpnI GATC 6 cut(s) 261, 324, 510, 576, 983, 990
DpnII GATC 6 cut(s) 259, 322, 508, 574, 981, 988
DraIII CACNNNGTG 1 cut(s) 584
EaeI YGGCCR 1 cut(s) 569
Eam1104I CTCTTC 1 cut(s) 701
EarI CTCTTC 1 cut(s) 701
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 3 cut(s) 138, 197, 450
Eco147I AGGCCT 1 cut(s) 184
Eco24I GRGCYC 2 cut(s) 28, 471
Eco47I GGWCC 3 cut(s) 155, 770, 869
Eco53kI GAGCTC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 155, 641
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 3 cut(s) 138, 197, 450
EcoT38I GRGCYC 2 cut(s) 28, 471
ErhI CCWWGG 3 cut(s) 138, 197, 450
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 4 cut(s) 266, 401, 803, 979
FalI AAGNNNNNCTT 4 cut(s) 455, 487, 618, 650
FaqI GGGAC 1 cut(s) 168
FatI CATG 4 cut(s) 262, 397, 799, 975
FauNDI CATATG 1 cut(s) 161
FbaI TGATCA 1 cut(s) 259
FokI GGATG 4 cut(s) 160, 416, 703, 896
FriOI GRGCYC 2 cut(s) 28, 471
FspBI CTAG 2 cut(s) 144, 198
GsuI CTGGAG 1 cut(s) 845
HaeIII GGCC 4 cut(s) 184, 571, 642, 804
HapII CCGG 4 cut(s) 317, 572, 796, 922
Hin1II CATG 4 cut(s) 266, 401, 803, 979
HinfI GANTC 2 cut(s) 588, 851
HpaII CCGG 4 cut(s) 317, 572, 796, 922
HphI GGTGA 1 cut(s) 426
Hpy166II GTNNAC 2 cut(s) 375, 649
Hpy188I TCNGA 2 cut(s) 36, 1019
Hpy188III TCNNGA 2 cut(s) 311, 425
Hpy8I GTNNAC 2 cut(s) 375, 649
HpyAV CCTTC 4 cut(s) 123, 457, 488, 667
HpyCH4III ACNGT 2 cut(s) 346, 785
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 4 cut(s) 165, 188, 483, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 466, 801
HpyF3I CTNAG 3 cut(s) 192, 288, 456
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 4 cut(s) 266, 401, 803, 979
KflI GGGWCCC 1 cut(s) 155
KpnI GGTACC 1 cut(s) 784
KroI GCCGGC 2 cut(s) 316, 795
KroNI GCCGGC 2 cut(s) 318, 797
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 6 cut(s) 259, 322, 508, 574, 981, 988
LguI GCTCTTC 1 cut(s) 701
LmnI GCTCC 4 cut(s) 23, 466, 935, 947
LweI GCATC 5 cut(s) 197, 295, 394, 490, 681
MaeI CTAG 2 cut(s) 144, 198
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 2 cut(s) 415, 823
MalI GATC 6 cut(s) 261, 324, 510, 576, 983, 990
MboI GATC 6 cut(s) 259, 322, 508, 574, 981, 988
MboII GAAGA 2 cut(s) 688, 722
MflI RGATCY 2 cut(s) 508, 574
MhlI GDGCHC 2 cut(s) 28, 471
MluCI AATT 5 cut(s) 82, 133, 765, 927, 993
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 9 cut(s) 187, 283, 353, 499, 614, 700, 867, 874, 882
MroNI GCCGGC 2 cut(s) 316, 795
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 2 cut(s) 753, 842
MslI CAYNNNNRTG 2 cut(s) 402, 974
MspI CCGG 4 cut(s) 317, 572, 796, 922
MspR9I CCNGG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 2 cut(s) 466, 801
NaeI GCCGGC 2 cut(s) 318, 797
NdeI CATATG 1 cut(s) 161
NdeII GATC 6 cut(s) 259, 322, 508, 574, 981, 988
NgoMIV GCCGGC 2 cut(s) 316, 795
NlaIII CATG 4 cut(s) 266, 401, 803, 979
NlaIV GGNNCC 7 cut(s) 156, 157, 468, 576, 728, 782, 966
NmeAIII GCCGAG 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 823
NspI RCATGY 2 cut(s) 401, 979
PasI CCCWGGG 1 cut(s) 275
PceI AGGCCT 1 cut(s) 184
PciI ACATGT 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 701
PctI GAATGC 1 cut(s) 485
PdiI GCCGGC 2 cut(s) 318, 797
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 2 cut(s) 588, 851
PflMI CCANNNNNTGG 1 cut(s) 584
PpuMI RGGWCCY 1 cut(s) 155
PscI ACATGT 1 cut(s) 975
Psp124BI GAGCTC 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 7 cut(s) 156, 157, 468, 576, 728, 782, 966
PspPI GGNCC 4 cut(s) 155, 641, 770, 869
PspPPI RGGWCCY 1 cut(s) 155
PstI CTGCAG 1 cut(s) 946
PsuI RGATCY 2 cut(s) 508, 574
RsaI GTAC 5 cut(s) 367, 566, 609, 650, 782
RsaNI GTAC 5 cut(s) 366, 565, 608, 649, 781
RseI CAYNNNNRTG 2 cut(s) 402, 974
SacI GAGCTC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 701
SaqAI TTAA 2 cut(s) 753, 842
Sau3AI GATC 6 cut(s) 259, 322, 508, 574, 981, 988
Sau96I GGNCC 4 cut(s) 155, 641, 770, 869
ScaI AGTACT 1 cut(s) 566
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 2 cut(s) 28, 471
SfaNI GCATC 5 cut(s) 197, 295, 394, 490, 681
SfcI CTRYAG 3 cut(s) 235, 249, 942
SinI GGWCC 3 cut(s) 155, 770, 869
SmiMI CAYNNNNRTG 2 cut(s) 402, 974
SmlI CTYRAG 2 cut(s) 178, 311
SmoI CTYRAG 2 cut(s) 178, 311
Sse9I AATT 5 cut(s) 82, 133, 765, 927, 993
SseBI AGGCCT 1 cut(s) 184
SsiI CCGC 2 cut(s) 437, 533
SspMI CTAG 2 cut(s) 144, 198
SstI GAGCTC 1 cut(s) 28
StuI AGGCCT 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 3 cut(s) 138, 197, 450
TaaI ACNGT 2 cut(s) 346, 785
TaiI ACGT 1 cut(s) 687
TasI AATT 5 cut(s) 82, 133, 765, 927, 993
TatI WGTACW 1 cut(s) 564
TfiI GAWTC 2 cut(s) 588, 851
Tru1I TTAA 2 cut(s) 753, 842
Tru9I TTAA 2 cut(s) 753, 842
TscAI CASTG 3 cut(s) 172, 351, 387
TseFI GTSAC 1 cut(s) 823
Tsp45I GTSAC 1 cut(s) 823
TspRI CASTG 3 cut(s) 172, 351, 387
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 3 cut(s) 155, 770, 869
XapI RAATTY 2 cut(s) 82, 133
XceI RCATGY 2 cut(s) 401, 979
XmaJI CCTAGG 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 2 cut(s) 144, 198
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.