MD13G1201900.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. MPBQ MBSQ MT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002098
Physical Location & Seq
Reverse (-)
18118912 .. 18122645
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1201900.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCGATTTCGATGCTTTACGGAGCTCAGAATCTCAAACTTGTCAATGGAATAATAGCAGTAACACCAAGTGGGTTAGGTTCTGGTGGGAAAAATTACCCTTATGGGGGTTTTTCCCAGAAGGGTTTAGTGAGCTATGGCGGTAGGAGTCAAAAGACCAGAACTTTAGTGGGACCCATATGCAGTGTATCATCCTCAAGGCCTGCTTCCCAGCCTAGGTTCATACAGCACAAGCAGGAGGCTTTCTGGTTCTATAGGTTTTTGTCTATAGTGTATGATCATGTTATAAACCCTGGGCATTGGACTGAGGATATGCGGGATGATGCGCTCGAGCCCGCTGAACTTTACGATAGGAATATGGTGGTGGTGGATGTTGGAGGTGGAACTGGGTTTACGACTCTTGGCATTGTTAAGCATGTGGATGCCAAGAATGTGACCATTCTTGACCAATCGCCGCACCAACTTGCCAAGGCTAAGGAGAAGGAGCCCTTGAAAGAATGCAAGATAGTTGAAGGGGATGCCGAGGATCTGCCGTTCCCAACTGATTACGCGGATCGATATGTCTCTGCTGGGAGTATTGAGTACTGGCCAGACCCACAGCGTGGAATCAAGGAAGCATATAGGGTACTAAAAATAGGAGGAAGGGCTTGCCTTATAGGCCCTGTGCACCCAACCTTTTGGCTGTCTCGTTTCTTTGCAGACGTGTGGATGCTCTTCCCAAAGGAGGAAGAGTACATTGAGTGGTTCAAAAATGCTGGTTTCAAAGATGTTCAACTGAAAAGAATTGGTCCAAAATGGTACCGTGGTGTCCGCCGCCATGGCTTGATCATGGGTTGCTCTGTGACAGGTGTTAAGCCCGTCTCTGGGGACTCCCCTCTCCAGCTTGGTCCAAAGGTGGAGGATGTCAGGAAGCCTGTGAATCCATTGGTGTTTCTTTTTCGGCTCATTCTCGGTGCAACTGCAGGAGCTTACTATGTGCTGGTTCCAATTTACATGTGGATTAAAGATCAAATTGTTCCGAAGGGCCAGCCTATCTGA

Protein Analysis

346

Amino Acids

38.55

Weight (kDa)

9.12

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 117 - 222 2.2e-12 Methyltransferase domain
Ubie_methyltran PF01209 119 - 230 1e-12 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 122 - 214 3.2e-17 Methyltransferase domain
Methyltransf_11 PF08241 123 - 217 9.7e-20 Methyltransferase domain
Methyltransf_12 PF08242 123 - 215 1.3e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
Acc65I GGTACC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 798
AccB7I CCANNNNNTGG 1 cut(s) 602
AccII CGCG 1 cut(s) 551
AciI CCGC 7 cut(s) 141, 316, 336, 455, 551, 811, 814
AclWI GGATC 2 cut(s) 534, 561
AcoI YGGCCR 1 cut(s) 587
AdeI CACNNNGTG 2 cut(s) 71, 602
AfaI GTAC 4 cut(s) 584, 627, 734, 800
AfiI CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
AflIII ACRYGT 2 cut(s) 702, 993
AgsI TTSAA 5 cut(s) 493, 512, 748, 763, 773
AjiI CACGTC 1 cut(s) 703
AjnI CCWGG 1 cut(s) 292
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 4 cut(s) 26, 135, 883, 968
AluI AGCT 4 cut(s) 26, 135, 883, 968
Alw21I GWGCWC 2 cut(s) 28, 669
Alw26I GTCTC 3 cut(s) 568, 690, 865
Alw44I GTGCAC 1 cut(s) 665
AlwI GGATC 2 cut(s) 534, 561
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 4 cut(s) 200, 587, 658, 1024
ApaLI GTGCAC 1 cut(s) 665
Asp718I GGTACC 1 cut(s) 798
AspA2I CCTAGG 1 cut(s) 215
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 5 cut(s) 173, 659, 788, 887, 1024
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 3 cut(s) 173, 788, 887
AvrII CCTAGG 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 669
BaeI ACNNNNGTAYC 2 cut(s) 790, 823
BalI TGGCCA 1 cut(s) 589
BanI GGYRCC 1 cut(s) 798
BanII GRGCYC 3 cut(s) 28, 336, 489
Bbv12I GWGCWC 2 cut(s) 28, 669
BceAI ACGGC 1 cut(s) 517
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 294
BclI TGATCA 2 cut(s) 277, 825
BcoDI GTCTC 3 cut(s) 568, 690, 865
BfaI CTAG 1 cut(s) 216
BfmI CTRYAG 3 cut(s) 253, 267, 960
BglI GCCNNNNNGGC 2 cut(s) 657, 819
BisI GCNGC 2 cut(s) 455, 814
BlnI CCTAGG 1 cut(s) 215
BlsI GCNGC 2 cut(s) 456, 815
BmcAI AGTACT 1 cut(s) 584
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 3 cut(s) 173, 788, 887
BmeT110I CYCGRG 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 703
BmgT120I GGNCC 5 cut(s) 173, 659, 788, 887, 1024
BmiI GGNNCC 5 cut(s) 174, 175, 486, 800, 984
BmrFI CCNGG 1 cut(s) 294
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 5 cut(s) 3, 313, 412, 508, 699
BmuI ACTGGG 1 cut(s) 396
BpmI CTGGAG 1 cut(s) 863
Bpu10I CCTNAGC 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 181
Bsa29I ATCGAT 1 cut(s) 556
BsaJI CCNNGG 7 cut(s) 215, 292, 293, 468, 522, 802, 817
BsaXI ACNNNNNCTCC 2 cut(s) 716, 746
Bsc4I CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
Bse1I ACTGG 2 cut(s) 391, 590
BseBI CCWGG 1 cut(s) 294
BseCI ATCGAT 1 cut(s) 556
BseDI CCNNGG 7 cut(s) 215, 292, 293, 468, 522, 802, 817
BseGI GGATG 7 cut(s) 191, 325, 376, 427, 523, 714, 907
BseLI CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
BseMII CTCAG 2 cut(s) 41, 297
BseNI ACTGG 2 cut(s) 391, 590
BseSI GKGCMC 1 cut(s) 669
BseYI CCCAGC 2 cut(s) 210, 569
Bsh1236I CGCG 1 cut(s) 551
BshFI GGCC 4 cut(s) 202, 589, 660, 1026
BshNI GGYRCC 1 cut(s) 798
BshVI ATCGAT 1 cut(s) 556
BsiHKAI GWGCWC 2 cut(s) 28, 669
BsiHKCI CYCGRG 1 cut(s) 329
BslFI GGGAC 2 cut(s) 186, 881
BslI CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
BsmAI GTCTC 3 cut(s) 568, 690, 865
BsmBI CGTCTC 1 cut(s) 865
BsmFI GGGAC 2 cut(s) 186, 881
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 4 cut(s) 202, 589, 660, 1026
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 4 cut(s) 28, 336, 489, 669
Bsp143I GATC 5 cut(s) 277, 526, 553, 825, 1006
Bsp19I CCATGG 1 cut(s) 817
BspACI CCGC 7 cut(s) 141, 316, 336, 455, 551, 811, 814
BspANI GGCC 4 cut(s) 202, 589, 660, 1026
BspCNI CTCAG 2 cut(s) 40, 298
BspDI ATCGAT 1 cut(s) 556
BspFNI CGCG 1 cut(s) 551
BspLI GGNNCC 5 cut(s) 174, 175, 486, 800, 984
BspMAI CTGCAG 1 cut(s) 964
BspPI GGATC 2 cut(s) 534, 561
BspQI GCTCTTC 1 cut(s) 719
BspT107I GGYRCC 1 cut(s) 798
BsrI ACTGG 2 cut(s) 391, 590
BssECI CCNNGG 7 cut(s) 215, 292, 293, 468, 522, 802, 817
BssMI GATC 5 cut(s) 277, 526, 553, 825, 1006
BssT1I CCWWGG 3 cut(s) 215, 468, 817
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 803
Bst6I CTCTTC 2 cut(s) 719, 723
BstC8I GCNNGC 4 cut(s) 204, 336, 649, 1028
BstDEI CTNAG 3 cut(s) 27, 306, 474
BstDSI CCRYGG 2 cut(s) 802, 817
BstF5I GGATG 7 cut(s) 191, 325, 376, 427, 523, 714, 907
BstFNI CGCG 1 cut(s) 551
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 5 cut(s) 280, 529, 556, 828, 1009
BstMAI GTCTC 3 cut(s) 568, 690, 865
BstMBI GATC 5 cut(s) 277, 526, 553, 825, 1006
BstMWI GCNNNNNNNGC 2 cut(s) 657, 819
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 2 cut(s) 419, 997
BstSCI CCNGG 1 cut(s) 292
BstSFI CTRYAG 3 cut(s) 253, 267, 960
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 526
BstXI CCANNNNNNTGG 1 cut(s) 678
BstYI RGATCY 1 cut(s) 526
Bsu15I ATCGAT 1 cut(s) 556
BsuRI GGCC 4 cut(s) 202, 589, 660, 1026
BsuTUI ATCGAT 1 cut(s) 556
BtgI CCRYGG 2 cut(s) 802, 817
BtrI CACGTC 1 cut(s) 703
BtsCI GGATG 7 cut(s) 191, 325, 376, 427, 523, 714, 907
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 190
Cac8I GCNNGC 4 cut(s) 204, 336, 649, 1028
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 5 cut(s) 173, 659, 788, 887, 1024
ClaI ATCGAT 1 cut(s) 556
Csp6I GTAC 4 cut(s) 583, 626, 733, 799
CviAII CATG 5 cut(s) 281, 416, 818, 829, 994
CviQI GTAC 4 cut(s) 583, 626, 733, 799
DdeI CTNAG 3 cut(s) 27, 306, 474
DpnI GATC 5 cut(s) 279, 528, 555, 827, 1008
DpnII GATC 5 cut(s) 277, 526, 553, 825, 1006
DraIII CACNNNGTG 2 cut(s) 71, 602
EaeI YGGCCR 1 cut(s) 587
Eam1104I CTCTTC 2 cut(s) 719, 723
EarI CTCTTC 2 cut(s) 719, 723
EciI GGCGGA 1 cut(s) 800
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 3 cut(s) 215, 468, 817
Eco147I AGGCCT 1 cut(s) 202
Eco24I GRGCYC 3 cut(s) 28, 336, 489
Eco47I GGWCC 3 cut(s) 173, 788, 887
Eco53kI GAGCTC 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 173, 659
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 3 cut(s) 215, 468, 817
EcoT38I GRGCYC 3 cut(s) 28, 336, 489
ErhI CCWWGG 3 cut(s) 215, 468, 817
Esp3I CGTCTC 1 cut(s) 865
FaeI CATG 5 cut(s) 284, 419, 821, 832, 997
FalI AAGNNNNNCTT 4 cut(s) 190, 222, 473, 505
FaqI GGGAC 2 cut(s) 186, 881
FatI CATG 5 cut(s) 280, 415, 817, 828, 993
FauI CCCGC 2 cut(s) 309, 343
FauNDI CATATG 1 cut(s) 179
FbaI TGATCA 2 cut(s) 277, 825
Fnu4HI GCNGC 2 cut(s) 455, 814
FokI GGATG 7 cut(s) 178, 332, 383, 434, 530, 721, 914
FriOI GRGCYC 3 cut(s) 28, 336, 489
Fsp4HI GCNGC 2 cut(s) 455, 814
FspBI CTAG 1 cut(s) 216
GlaI GCGC 1 cut(s) 327
GluI GCNGC 2 cut(s) 455, 814
GsaI CCCAGC 2 cut(s) 214, 573
GsuI CTGGAG 1 cut(s) 863
HaeIII GGCC 4 cut(s) 202, 589, 660, 1026
HhaI GCGC 1 cut(s) 328
Hin1II CATG 5 cut(s) 284, 419, 821, 832, 997
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HinfI GANTC 6 cut(s) 31, 148, 397, 606, 869, 919
Hpy166II GTNNAC 2 cut(s) 393, 667
Hpy188I TCNGA 3 cut(s) 30, 1020, 1037
Hpy188III TCNNGA 2 cut(s) 443, 907
Hpy8I GTNNAC 2 cut(s) 393, 667
HpyAV CCTTC 5 cut(s) 115, 475, 506, 636, 1015
HpyCH4III ACNGT 1 cut(s) 803
HpyCH4IV ACGT 1 cut(s) 702
HpyCH4V TGCA 6 cut(s) 183, 501, 667, 698, 956, 962
HpyF10VI GCNNNNNNNGC 2 cut(s) 657, 819
HpyF3I CTNAG 3 cut(s) 27, 306, 474
HpySE526I ACGT 1 cut(s) 702
Hsp92II CATG 5 cut(s) 284, 419, 821, 832, 997
HspAI GCGC 1 cut(s) 326
KflI GGGWCCC 1 cut(s) 173
KpnI GGTACC 1 cut(s) 802
Ksp22I TGATCA 2 cut(s) 277, 825
Kzo9I GATC 5 cut(s) 277, 526, 553, 825, 1006
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 3 cut(s) 23, 484, 965
LweI GCATC 5 cut(s) 3, 313, 412, 508, 699
MaeI CTAG 1 cut(s) 216
MaeII ACGT 1 cut(s) 702
MaeIII GTNAC 3 cut(s) 61, 433, 841
MalI GATC 5 cut(s) 279, 528, 555, 827, 1008
MboI GATC 5 cut(s) 277, 526, 553, 825, 1006
MboII GAAGA 2 cut(s) 706, 740
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 4 cut(s) 28, 336, 489, 669
MlsI TGGCCA 1 cut(s) 589
MluCI AATT 4 cut(s) 94, 783, 987, 1011
MluNI TGGCCA 1 cut(s) 589
MlyI GAGTC 3 cut(s) 157, 391, 863
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 9 cut(s) 205, 232, 301, 371, 517, 632, 718, 885, 892
Mox20I TGGCCA 1 cut(s) 589
MscI TGGCCA 1 cut(s) 589
MseI TTAA 3 cut(s) 411, 852, 1002
MslI CAYNNNNRTG 1 cut(s) 420
Msp20I TGGCCA 1 cut(s) 589
MspA1I CMGCKG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 294
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 551
MwoI GCNNNNNNNGC 2 cut(s) 657, 819
NcoI CCATGG 1 cut(s) 817
NdeI CATATG 1 cut(s) 179
NdeII GATC 5 cut(s) 277, 526, 553, 825, 1006
NlaIII CATG 5 cut(s) 284, 419, 821, 832, 997
NlaIV GGNNCC 5 cut(s) 174, 175, 486, 800, 984
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 433, 841
NspI RCATGY 2 cut(s) 419, 997
PaeR7I CTCGAG 1 cut(s) 329
PasI CCCWGGG 1 cut(s) 293
PceI AGGCCT 1 cut(s) 202
PciI ACATGT 1 cut(s) 993
PciSI GCTCTTC 1 cut(s) 719
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 3 cut(s) 31, 606, 919
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 2 cut(s) 456, 815
PleI GAGTC 3 cut(s) 156, 391, 863
PpsI GAGTC 3 cut(s) 156, 391, 863
PpuMI RGGWCCY 1 cut(s) 173
PscI ACATGT 1 cut(s) 993
PsiI TTATAA 1 cut(s) 287
Psp124BI GAGCTC 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 292
PspFI CCCAGC 2 cut(s) 210, 569
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 5 cut(s) 174, 175, 486, 800, 984
PspPI GGNCC 5 cut(s) 173, 659, 788, 887, 1024
PspPPI RGGWCCY 1 cut(s) 173
PspXI VCTCGAGB 1 cut(s) 329
PsrI GAACNNNNNNTAC 2 cut(s) 376, 408
PstI CTGCAG 1 cut(s) 964
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 4 cut(s) 584, 627, 734, 800
RsaNI GTAC 4 cut(s) 583, 626, 733, 799
RseI CAYNNNNRTG 1 cut(s) 420
SacI GAGCTC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 719
SaqAI TTAA 3 cut(s) 411, 852, 1002
SatI GCNGC 2 cut(s) 455, 814
Sau3AI GATC 5 cut(s) 277, 526, 553, 825, 1006
Sau96I GGNCC 5 cut(s) 173, 659, 788, 887, 1024
ScaI AGTACT 1 cut(s) 584
SchI GAGTC 3 cut(s) 157, 391, 863
ScrFI CCNGG 1 cut(s) 294
SduI GDGCHC 4 cut(s) 28, 336, 489, 669
SfaNI GCATC 5 cut(s) 3, 313, 412, 508, 699
SfcI CTRYAG 3 cut(s) 253, 267, 960
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 3 cut(s) 173, 788, 887
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 2 cut(s) 196, 329
SmoI CTYRAG 2 cut(s) 196, 329
Sse9I AATT 4 cut(s) 94, 783, 987, 1011
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 7 cut(s) 141, 316, 336, 455, 551, 811, 814
SspMI CTAG 1 cut(s) 216
SstI GAGCTC 1 cut(s) 28
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 3 cut(s) 215, 468, 817
TaaI ACNGT 1 cut(s) 803
TaiI ACGT 1 cut(s) 705
TaqI TCGA 3 cut(s) 11, 330, 556
TasI AATT 4 cut(s) 94, 783, 987, 1011
TatI WGTACW 2 cut(s) 582, 732
TauI GCSGC 2 cut(s) 457, 816
TfiI GAWTC 3 cut(s) 31, 606, 919
Tru1I TTAA 3 cut(s) 411, 852, 1002
Tru9I TTAA 3 cut(s) 411, 852, 1002
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 2 cut(s) 433, 841
Tsp45I GTSAC 2 cut(s) 433, 841
TspDTI ATGAA 1 cut(s) 211
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 1 cut(s) 190
Van91I CCANNNNNTGG 1 cut(s) 602
VneI GTGCAC 1 cut(s) 665
VpaK11BI GGWCC 3 cut(s) 173, 788, 887
XceI RCATGY 2 cut(s) 419, 997
XcmI CCANNNNNNNNNTGG 2 cut(s) 166, 993
XhoI CTCGAG 1 cut(s) 329
XmaJI CCTAGG 1 cut(s) 215
XspI CTAG 1 cut(s) 216
ZrmI AGTACT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.