Rroxscaffold_5G00335090
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. MPBQ MBSQ MT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002098
Physical Location & Seq
Reverse (-)
2571772 .. 2574752
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00335090.1

Sequence Viewer

Length: 759 bp
ATGGCTATTTCGATGCTCTATGGAGCTCAAATTCTGAAACTCACTTCTGGCATAGCACCAACTGGGTTAGGTTCTGGGGGAAATTTTCAATATGGGTCTTTCCCCAAAAAGGTTTCAGTTAGTTATGGAAGGAATTCCAAGGCTAGAACTTTGGGACCCATATGCAGTGTCTCATCCTCAAGGCCTGCTTCTCAACCTAGGTTCATACAGCACAAGCAAGAAGCATTTTGGTTTTACAGGTTTTTATCTATAGTCTATGATCATGTGATAAACCCTGGGCATTGGACTGAGGATATGAGAGACGATGCTCTTGAGCCGGCTGATTTTTATGACAGGAATATGACAGTGGTAGATGTTGGAGGTGGTACTGGGTTCACAACACTGGGGATTGTGAAGCATGTGGATGCCAAAAATGTTACTATTCTTGACCAATCACCGCACCAGCTTGCCAAGGTTAAGCAGAAGGAGCCCTTGAGAGAGTGCAAGATAATTGAAGGTGATGCGGAGGATCTGCCATTCCCAACTGATTATGCGGATAGATATGTCTCTGCTGGAAGTATAGAGTACTGGCCGGATCCACAGCGTGGAATCAAGGAAGCATACAGGGTACTAAAAATAGGAGGAAAAGCCTGTCTTATAGGTCCTGTGTACCCAACCTTCTGGCTGTCTCGTTTTTCGCAGACGTGTGGATGCTCTTCCCAACAGAGGAAGAATACATTGAGTGGTTCCAAAAGGCAGGTTTCAAAGATGTTAAACTAA

Protein Analysis

252

Amino Acids

28.0

Weight (kDa)

9.21

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 111 - 216 3.5e-11 Methyltransferase domain
Ubie_methyltran PF01209 112 - 213 6.8e-12 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 116 - 208 1e-16 Methyltransferase domain
Methyltransf_11 PF08241 117 - 211 2.2e-19 Methyltransferase domain
Methyltransf_12 PF08242 117 - 209 3.1e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 727
AccB7I CCANNNNNTGG 1 cut(s) 584
AciI CCGC 3 cut(s) 437, 503, 533
AclWI GGATC 3 cut(s) 516, 569, 582
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 3 cut(s) 30, 82, 133
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 4 cut(s) 367, 566, 609, 650
AfiI CCNNNNNNNGG 3 cut(s) 109, 584, 705
AflIII ACRYGT 1 cut(s) 683
AgsI TTSAA 3 cut(s) 89, 494, 744
AjiI CACGTC 1 cut(s) 684
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 2 cut(s) 26, 445
AluI AGCT 2 cut(s) 26, 445
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 4 cut(s) 175, 294, 550, 672
AlwI GGATC 3 cut(s) 516, 569, 582
AoxI GGCC 2 cut(s) 182, 569
ApoI RAATTY 3 cut(s) 30, 82, 133
Asp700I GAANNNNTTC 1 cut(s) 133
AspA2I CCTAGG 1 cut(s) 197
AspS9I GGNCC 2 cut(s) 155, 641
AsuHPI GGTGA 2 cut(s) 426, 509
AvaII GGWCC 2 cut(s) 155, 641
AvrII CCTAGG 1 cut(s) 197
BamHI GGATCC 1 cut(s) 574
BanII GRGCYC 2 cut(s) 28, 471
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
Bbv12I GWGCWC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 276
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 4 cut(s) 175, 294, 550, 672
BfaI CTAG 2 cut(s) 144, 198
BfmI CTRYAG 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 727
BlnI CCTAGG 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 2 cut(s) 155, 641
BmgBI CACGTC 1 cut(s) 684
BmgT120I GGNCC 2 cut(s) 155, 641
BmiI GGNNCC 5 cut(s) 156, 157, 468, 576, 727
BmrFI CCNGG 1 cut(s) 276
BmrI ACTGGG 3 cut(s) 72, 378, 392
BmsI GCATC 5 cut(s) 3, 295, 394, 490, 680
BmuI ACTGGG 3 cut(s) 72, 378, 392
BpuEI CTTGAG 3 cut(s) 163, 332, 493
BsaJI CCNNGG 5 cut(s) 138, 197, 274, 275, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 584, 705
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 4 cut(s) 67, 373, 387, 572
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 5 cut(s) 138, 197, 274, 275, 450
BseGI GGATG 3 cut(s) 173, 409, 695
BseLI CCNNNNNNNGG 3 cut(s) 109, 584, 705
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 4 cut(s) 67, 373, 387, 572
BshFI GGCC 2 cut(s) 184, 571
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 2 cut(s) 317, 572
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 3 cut(s) 109, 584, 705
BsmAI GTCTC 4 cut(s) 175, 294, 550, 672
BsmBI CGTCTC 1 cut(s) 294
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 2 cut(s) 184, 571
Bsp1286I GDGCHC 2 cut(s) 28, 471
Bsp143I GATC 3 cut(s) 259, 508, 574
BspACI CCGC 3 cut(s) 437, 503, 533
BspANI GGCC 2 cut(s) 184, 571
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 5 cut(s) 156, 157, 468, 576, 727
BspMI ACCTGC 1 cut(s) 727
BspPI GGATC 3 cut(s) 516, 569, 582
BspQI GCTCTTC 1 cut(s) 700
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 4 cut(s) 67, 373, 387, 572
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 5 cut(s) 138, 197, 274, 275, 450
BssMI GATC 3 cut(s) 259, 508, 574
BssT1I CCWWGG 3 cut(s) 138, 197, 450
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 1 cut(s) 700
BstC8I GCNNGC 3 cut(s) 186, 318, 447
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 3 cut(s) 173, 409, 695
BstKTI GATC 3 cut(s) 262, 511, 577
BstMAI GTCTC 4 cut(s) 175, 294, 550, 672
BstMBI GATC 3 cut(s) 259, 508, 574
BstMWI GCNNNNNNNGC 1 cut(s) 466
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 274
BstSFI CTRYAG 1 cut(s) 249
BstX2I RGATCY 2 cut(s) 508, 574
BstXI CCANNNNNNTGG 1 cut(s) 660
BstYI RGATCY 2 cut(s) 508, 574
BsuRI GGCC 2 cut(s) 184, 571
BtrI CACGTC 1 cut(s) 684
BtsCI GGATG 3 cut(s) 173, 409, 695
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 3 cut(s) 172, 351, 380
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 3 cut(s) 186, 318, 447
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 155, 641
Csp6I GTAC 4 cut(s) 366, 565, 608, 649
CviAII CATG 2 cut(s) 263, 398
CviQI GTAC 4 cut(s) 366, 565, 608, 649
DdeI CTNAG 1 cut(s) 288
DpnI GATC 3 cut(s) 261, 510, 576
DpnII GATC 3 cut(s) 259, 508, 574
DraIII CACNNNGTG 1 cut(s) 584
EaeI YGGCCR 1 cut(s) 569
Eam1104I CTCTTC 1 cut(s) 700
EarI CTCTTC 1 cut(s) 700
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 3 cut(s) 138, 197, 450
Eco147I AGGCCT 1 cut(s) 184
Eco24I GRGCYC 2 cut(s) 28, 471
Eco47I GGWCC 2 cut(s) 155, 641
Eco53kI GAGCTC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 155, 641
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 3 cut(s) 138, 197, 450
EcoT38I GRGCYC 2 cut(s) 28, 471
ErhI CCWWGG 3 cut(s) 138, 197, 450
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 2 cut(s) 266, 401
FalI AAGNNNNNCTT 6 cut(s) 172, 204, 455, 487, 618, 650
FaqI GGGAC 1 cut(s) 168
FatI CATG 2 cut(s) 262, 397
FauNDI CATATG 1 cut(s) 161
FbaI TGATCA 1 cut(s) 259
FokI GGATG 3 cut(s) 160, 416, 702
FriOI GRGCYC 2 cut(s) 28, 471
FspBI CTAG 2 cut(s) 144, 198
HaeIII GGCC 2 cut(s) 184, 571
HapII CCGG 2 cut(s) 317, 572
Hin1II CATG 2 cut(s) 266, 401
HinfI GANTC 1 cut(s) 588
HpaII CCGG 2 cut(s) 317, 572
HphI GGTGA 2 cut(s) 426, 509
Hpy166II GTNNAC 2 cut(s) 375, 649
Hpy188I TCNGA 1 cut(s) 36
Hpy188III TCNNGA 2 cut(s) 311, 425
Hpy8I GTNNAC 2 cut(s) 375, 649
HpyAV CCTTC 4 cut(s) 123, 457, 488, 667
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 683
HpyCH4V TGCA 2 cut(s) 165, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 466
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 683
Hsp92II CATG 2 cut(s) 266, 401
KflI GGGWCCC 1 cut(s) 155
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 259, 508, 574
LguI GCTCTTC 1 cut(s) 700
LmnI GCTCC 2 cut(s) 23, 466
LweI GCATC 5 cut(s) 3, 295, 394, 490, 680
MaeI CTAG 2 cut(s) 144, 198
MaeII ACGT 1 cut(s) 683
MaeIII GTNAC 1 cut(s) 415
MalI GATC 3 cut(s) 261, 510, 576
MboI GATC 3 cut(s) 259, 508, 574
MboII GAAGA 2 cut(s) 687, 721
MflI RGATCY 2 cut(s) 508, 574
MhlI GDGCHC 2 cut(s) 28, 471
MluCI AATT 4 cut(s) 30, 82, 133, 489
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 6 cut(s) 187, 283, 353, 499, 614, 699
MroNI GCCGGC 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 2 cut(s) 456, 752
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 2 cut(s) 317, 572
MspR9I CCNGG 1 cut(s) 276
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 466
NaeI GCCGGC 1 cut(s) 318
NdeI CATATG 1 cut(s) 161
NdeII GATC 3 cut(s) 259, 508, 574
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 2 cut(s) 266, 401
NlaIV GGNNCC 5 cut(s) 156, 157, 468, 576, 727
NspI RCATGY 1 cut(s) 401
PasI CCCWGGG 1 cut(s) 275
PceI AGGCCT 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 700
PdiI GCCGGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 588
PflMI CCANNNNNTGG 1 cut(s) 584
PpuMI RGGWCCY 2 cut(s) 155, 641
Psp124BI GAGCTC 1 cut(s) 28
Psp5II RGGWCCY 2 cut(s) 155, 641
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 5 cut(s) 156, 157, 468, 576, 727
PspPI GGNCC 2 cut(s) 155, 641
PspPPI RGGWCCY 2 cut(s) 155, 641
PsuI RGATCY 2 cut(s) 508, 574
RsaI GTAC 4 cut(s) 367, 566, 609, 650
RsaNI GTAC 4 cut(s) 366, 565, 608, 649
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 700
SaqAI TTAA 2 cut(s) 456, 752
Sau3AI GATC 3 cut(s) 259, 508, 574
Sau96I GGNCC 2 cut(s) 155, 641
ScaI AGTACT 1 cut(s) 566
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 2 cut(s) 28, 471
SfaNI GCATC 5 cut(s) 3, 295, 394, 490, 680
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 2 cut(s) 155, 641
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 3 cut(s) 178, 311, 472
SmoI CTYRAG 3 cut(s) 178, 311, 472
Sse9I AATT 4 cut(s) 30, 82, 133, 489
SseBI AGGCCT 1 cut(s) 184
SsiI CCGC 3 cut(s) 437, 503, 533
SspMI CTAG 2 cut(s) 144, 198
SstI GAGCTC 1 cut(s) 28
StuI AGGCCT 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 3 cut(s) 138, 197, 450
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 686
TaqI TCGA 1 cut(s) 11
TasI AATT 4 cut(s) 30, 82, 133, 489
TatI WGTACW 1 cut(s) 564
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 2 cut(s) 456, 752
Tru9I TTAA 2 cut(s) 456, 752
TscAI CASTG 3 cut(s) 172, 351, 387
TspDTI ATGAA 1 cut(s) 193
TspRI CASTG 3 cut(s) 172, 351, 387
Van91I CCANNNNNTGG 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 155, 641
XapI RAATTY 3 cut(s) 30, 82, 133
XceI RCATGY 1 cut(s) 401
XmaJI CCTAGG 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 2 cut(s) 144, 198
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.