Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0002098

Physical Location & Seq

Genomic Coordinates Forward (+)

55743576 .. 55746307

Gene Structure 2732 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG325600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1032 bp

ATGGCCTCTGCGATGCTCAACGGGGCCGAGCATCTCACACTCCGGCGAGGTTTATCCCCAAATGGGTTGGGTTTTGTTGGGTCGGATTTTCATGGGAAGCAGTTTCTGAAGATGGGTTTGGTCTCAAACACTAGAATTTCCAAGTCTGGTGCTAGAGCCATTGTGCCCAAGTGTAGTGTCTCACCTTCCAGGCCTGCTTCTCAGCCCAGGTTCATTCAGCACAAGAAAGAGGCTTTCTGGTTTTATAGGTTTCTGTCAATTGTTTATGACCATGTCATAAACCCTGGGCATTGGACTGAGGACATGAGAGACGAGGCACTTGAGCCAGCTGATCTCAGCGACAGGAGAATGATTGTCGTAGATGTGGGTGGAGGTACTGGGTTCACTACCTTGGGGATAGTTAAGCATGTGGATGCCAAGAATGTGACCATTCTTGATCAGTCCCCTCATCAGCTAGCTAGGGCTAAGAAGAAGGAGCCTTTGAAGGAGTGCAAGATCATTGAGGGCGATGCTGAGGACTTGCCTTTTCGGACTGATTATGCGGATCGATATGTATCTGCAGGAAGTATTGAGTACTGGCCAGACCCACAGCGTGGCATCAAGGAAGCATACAGGGTCTTGAAACTTGGAGGAAAAGCATGTGTGATTGGTCCTGTGTACCCAACGTTTTGGTTGTCTCGCTTCTTTGCTGATGTATGGATGCTTTTCCCCAAGGAGGAAGAGTACATTGAATGGTTCGAGAAGGCTGGATTCAAAGATGTCCAACTGAAAAGAATTGGCCCAAAATGGTACCGTGGGGTTCGCCGCCATGGATTGATAATGGGGTGTTCTGTAACAGGCGTTAAGCCTGCGTCTGGGGATTCTCCTTTACAGGTAGTTTGTTTTCGAGAGCTCTATTTTGACTTATTTCTTGATAATTATAATTGCTCTTCTGAGTTGGATTCCTTATCAGTTTATAGCCTTGAGATTTTATGTTTATGGATGCATATAATATCAACTTTGATGCAATCAGAAGCGCATCTAAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006732 GO:0006766 GO:0006775 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009058 GO:0009108 GO:0009110 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0009987 GO:0010189 GO:0010236 GO:0016020 GO:0016740 GO:0016741 GO:0018130 GO:0019866 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032259 GO:0042170 GO:0042180 GO:0042181 GO:0042360 GO:0042362 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0051186 GO:0051188 GO:0051741 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

Methyltransf_11 Methyltransf_25

Protein Analysis

343

Amino Acids

38.86

Weight (kDa)

8.36

Isoelectric Point (pI)

45.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_31	PF13847	114 - 219	3.4e-11	Methyltransferase domain
Ubie_methyltran	PF01209	117 - 221	3.3e-12	ubiE/COQ5 methyltransferase family
Methyltransf_25	PF13649	119 - 211	9.4e-17	Methyltransferase domain
Methyltransf_11	PF08241	120 - 214	2.5e-19	Methyltransferase domain
Methyltransf_12	PF08242	120 - 212	7.6e-10	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002098)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63410
fragaria_vesca	FvH4_2g22850 FvH4_4g01770
malus_domestica	MD03G1256900.v1.1 MD11G1277500.v1.1 MD13G1201900.v1.1 MD16G1202200.v1.1
prunus_persica	Prupe.1G023700_v2.0.a1 Prupe.1G023700_v2.0.a1 Prupe.1G023700_v2.0.a1 Prupe.1G023700_v2.0.a1 Prupe.8G233800_v2.0.a1
pyrus_communis	pycom03g20360 pycom13g17530 pycom16g17060
rosa_chinensis	RchiOBHm_Chr4g0388681 RchiOBHm_Chr4g0388811 RchiOBHm_Chr6g0289771
rosa_laevigata	RLG00000010027 RLG00000012268
rosa_multiflora	Rmu_co8119594.1_g000001 Rmu_sc0000697.1_g000014 Rmu_sc0004459.1_g000002 Rmu_sc0005640.1_g000008 Rmu_sc0016023.1_g000001 Rmu_sc0027068.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335090 Rroxscaffold_7G00177560 Rroxscaffold_7G00178080
rosa_rugosa	Rorug03G0320000 Rorug06G0206500 Rorug06G0206600
rosa_samantha	Rh4AG024800 Rh4BG017800 Rh4CG026800 Rh4CG027500 Rh4DG018100 Rh6AG318300 Rh6BG325600 Rh6CG331400 Rh6DG316900
rosa_wichuraiana	Rw4G001730 Rw4G001770 Rw6G027460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	921
Acc65I	GGTACC	1 cut(s)	789
AccB1I	GGYRCC	1 cut(s)	789
AccB7I	CCANNNNNTGG	1 cut(s)	593
AciI	CCGC	2 cut(s)	542, 805
AclI	AACGTT	1 cut(s)	665
AclWI	GGATC	1 cut(s)	552
AcoI	YGGCCR	1 cut(s)	578
AcsI	RAATTY	2 cut(s)	135, 1024
AcuI	CTGAAG	1 cut(s)	128
AdeI	CACNNNGTG	1 cut(s)	593
AfaI	GTAC	5 cut(s)	376, 575, 659, 725, 791
AfiI	CCNNNNNNNGG	4 cut(s)	63, 593, 715, 854
AgsI	TTSAA	4 cut(s)	484, 622, 731, 754
AjnI	CCWGG	3 cut(s)	188, 206, 283
AjuI	GAANNNNNNNTTGG	2 cut(s)	101, 133
AluBI	AGCT	4 cut(s)	329, 454, 458, 892
AluI	AGCT	4 cut(s)	329, 454, 458, 892
Alw21I	GWGCWC	1 cut(s)	894
Alw26I	GTCTC	4 cut(s)	127, 184, 303, 681
AlwI	GGATC	1 cut(s)	552
AlwNI	CAGNNNCTG	1 cut(s)	106
AoxI	GGCC	5 cut(s)	3, 24, 191, 578, 778
ApoI	RAATTY	2 cut(s)	135, 1024
Asp718I	GGTACC	1 cut(s)	789
AspLEI	GCGC	1 cut(s)	1018
AspS9I	GGNCC	3 cut(s)	24, 650, 779
AsuHPI	GGTGA	1 cut(s)	174
AsuNHI	GCTAGC	1 cut(s)	454
AvaII	GGWCC	1 cut(s)	650
BaeGI	GKGCMC	1 cut(s)	168
BalI	TGGCCA	1 cut(s)	580
BanI	GGYRCC	1 cut(s)	789
BanII	GRGCYC	1 cut(s)	894
Bbv12I	GWGCWC	1 cut(s)	894
BbvCI	CCTCAGC	1 cut(s)	513
BccI	CCATC	1 cut(s)	106
BciT130I	CCWGG	3 cut(s)	190, 208, 285
BclI	TGATCA	1 cut(s)	436
BcoDI	GTCTC	4 cut(s)	127, 184, 303, 681
BfaI	CTAG	4 cut(s)	132, 153, 455, 459
BfmI	CTRYAG	1 cut(s)	558
BisI	GCNGC	1 cut(s)	805
BlsI	GCNGC	1 cut(s)	806
BmcAI	AGTACT	1 cut(s)	575
Bme1390I	CCNGG	3 cut(s)	190, 208, 285
Bme18I	GGWCC	1 cut(s)	650
BmgT120I	GGNCC	3 cut(s)	24, 650, 779
BmiI	GGNNCC	3 cut(s)	25, 477, 791
BmrFI	CCNGG	3 cut(s)	190, 208, 285
BmrI	ACTGGG	1 cut(s)	387
BmsI	GCATC	9 cut(s)	3, 40, 403, 499, 606, 690, 972, 993, 1027
BmtI	GCTAGC	1 cut(s)	458
BmuI	ACTGGG	1 cut(s)	387
Bpu10I	CCTNAGC	1 cut(s)	513
BpuEI	CTTGAG	2 cut(s)	341, 983
Bsa29I	ATCGAT	1 cut(s)	547
BsaBI	GATNNNNATC	1 cut(s)	553
BsaI	GGTCTC	1 cut(s)	127
BsaJI	CCNNGG	7 cut(s)	206, 283, 284, 390, 711, 793, 808
BsaXI	ACNNNNNCTCC	2 cut(s)	707, 737
Bsc4I	CCNNNNNNNGG	4 cut(s)	63, 593, 715, 854
Bse1I	ACTGG	2 cut(s)	382, 581
Bse8I	GATNNNNATC	1 cut(s)	553
BseBI	CCWGG	3 cut(s)	190, 208, 285
BseCI	ATCGAT	1 cut(s)	547
BseDI	CCNNGG	7 cut(s)	206, 283, 284, 390, 711, 793, 808
BseGI	GGATG	3 cut(s)	418, 705, 987
BseJI	GATNNNNATC	1 cut(s)	553
BseLI	CCNNNNNNNGG	4 cut(s)	63, 593, 715, 854
BseMII	CTCAG	5 cut(s)	215, 288, 349, 504, 924
BseNI	ACTGG	2 cut(s)	382, 581
BseSI	GKGCMC	1 cut(s)	168
BshFI	GGCC	5 cut(s)	5, 26, 193, 580, 780
BshNI	GGYRCC	1 cut(s)	789
BshVI	ATCGAT	1 cut(s)	547
BsiHKAI	GWGCWC	1 cut(s)	894
BsiSI	CCGG	1 cut(s)	43
BslFI	GGGAC	1 cut(s)	427
BslI	CCNNNNNNNGG	4 cut(s)	63, 593, 715, 854
BsmAI	GTCTC	4 cut(s)	127, 184, 303, 681
BsmBI	CGTCTC	1 cut(s)	303
BsmFI	GGGAC	1 cut(s)	427
BsnI	GGCC	5 cut(s)	5, 26, 193, 580, 780
Bso31I	GGTCTC	1 cut(s)	127
Bsp1286I	GDGCHC	2 cut(s)	168, 894
Bsp143I	GATC	4 cut(s)	331, 436, 495, 544
Bsp19I	CCATGG	1 cut(s)	808
BspACI	CCGC	2 cut(s)	542, 805
BspANI	GGCC	5 cut(s)	5, 26, 193, 580, 780
BspCNI	CTCAG	5 cut(s)	214, 289, 348, 505, 925
BspDI	ATCGAT	1 cut(s)	547
BspLI	GGNNCC	3 cut(s)	25, 477, 791
BspMAI	CTGCAG	1 cut(s)	562
BspOI	GCTAGC	1 cut(s)	458
BspPI	GGATC	1 cut(s)	552
BspQI	GCTCTTC	1 cut(s)	934
BspT107I	GGYRCC	1 cut(s)	789
BspTNI	GGTCTC	1 cut(s)	127
BsrI	ACTGG	2 cut(s)	382, 581
BssECI	CCNNGG	7 cut(s)	206, 283, 284, 390, 711, 793, 808
BssMI	GATC	4 cut(s)	331, 436, 495, 544
BssT1I	CCWWGG	3 cut(s)	390, 711, 808
Bst2UI	CCWGG	3 cut(s)	190, 208, 285
Bst4CI	ACNGT	1 cut(s)	794
Bst6I	CTCTTC	2 cut(s)	714, 934
BstC8I	GCNNGC	4 cut(s)	195, 327, 456, 849
BstDEI	CTNAG	6 cut(s)	201, 297, 335, 465, 513, 933
BstDSI	CCRYGG	2 cut(s)	793, 808
BstF5I	GGATG	3 cut(s)	418, 705, 987
BstHHI	GCGC	1 cut(s)	1018
BstKTI	GATC	4 cut(s)	334, 439, 498, 547
BstMAI	GTCTC	4 cut(s)	127, 184, 303, 681
BstMBI	GATC	4 cut(s)	331, 436, 495, 544
BstNI	CCWGG	3 cut(s)	190, 208, 285
BstNSI	RCATGY	2 cut(s)	410, 642
BstSCI	CCNGG	3 cut(s)	188, 206, 283
BstSFI	CTRYAG	1 cut(s)	558
BstSLI	GKGCMC	1 cut(s)	168
BstXI	CCANNNNNNTGG	1 cut(s)	669
Bsu15I	ATCGAT	1 cut(s)	547
BsuRI	GGCC	5 cut(s)	5, 26, 193, 580, 780
BsuTUI	ATCGAT	1 cut(s)	547
BtgI	CCRYGG	2 cut(s)	793, 808
BtgZI	GCGATG	2 cut(s)	26, 522
BtsCI	GGATG	3 cut(s)	418, 705, 987
Cac8I	GCNNGC	4 cut(s)	195, 327, 456, 849
CaiI	CAGNNNCTG	1 cut(s)	106
CfoI	GCGC	1 cut(s)	1018
Cfr13I	GGNCC	3 cut(s)	24, 650, 779
ClaI	ATCGAT	1 cut(s)	547
CseI	GACGC	1 cut(s)	840
Csp6I	GTAC	5 cut(s)	375, 574, 658, 724, 790
CviAII	CATG	6 cut(s)	92, 272, 304, 407, 639, 809
CviQI	GTAC	5 cut(s)	375, 574, 658, 724, 790
DdeI	CTNAG	6 cut(s)	201, 297, 335, 465, 513, 933
DpnI	GATC	4 cut(s)	333, 438, 497, 546
DpnII	GATC	4 cut(s)	331, 436, 495, 544
DraIII	CACNNNGTG	1 cut(s)	593
EaeI	YGGCCR	1 cut(s)	578
Eam1104I	CTCTTC	2 cut(s)	714, 934
EarI	CTCTTC	2 cut(s)	714, 934
Ecl136II	GAGCTC	1 cut(s)	892
Eco130I	CCWWGG	3 cut(s)	390, 711, 808
Eco147I	AGGCCT	1 cut(s)	193
Eco24I	GRGCYC	1 cut(s)	894
Eco31I	GGTCTC	1 cut(s)	127
Eco47I	GGWCC	1 cut(s)	650
Eco53kI	GAGCTC	1 cut(s)	892
Eco57I	CTGAAG	1 cut(s)	128
EcoICRI	GAGCTC	1 cut(s)	892
EcoRII	CCWGG	3 cut(s)	188, 206, 283
EcoT14I	CCWWGG	3 cut(s)	390, 711, 808
EcoT22I	ATGCAT	1 cut(s)	987
EcoT38I	GRGCYC	1 cut(s)	894
ErhI	CCWWGG	3 cut(s)	390, 711, 808
Esp3I	CGTCTC	1 cut(s)	303
FaeI	CATG	6 cut(s)	95, 275, 307, 410, 642, 812
FaqI	GGGAC	1 cut(s)	427
FatI	CATG	6 cut(s)	91, 271, 303, 406, 638, 808
FbaI	TGATCA	1 cut(s)	436
Fnu4HI	GCNGC	1 cut(s)	805
FokI	GGATG	3 cut(s)	425, 712, 994
FriOI	GRGCYC	1 cut(s)	894
Fsp4HI	GCNGC	1 cut(s)	805
FspBI	CTAG	4 cut(s)	132, 153, 455, 459
GlaI	GCGC	1 cut(s)	1017
GluI	GCNGC	1 cut(s)	805
HaeIII	GGCC	5 cut(s)	5, 26, 193, 580, 780
HapII	CCGG	1 cut(s)	43
HgaI	GACGC	1 cut(s)	840
HhaI	GCGC	1 cut(s)	1018
Hin1II	CATG	6 cut(s)	95, 275, 307, 410, 642, 812
Hin6I	GCGC	1 cut(s)	1016
HinP1I	GCGC	1 cut(s)	1016
HinfI	GANTC	3 cut(s)	750, 860, 941
HpaII	CCGG	1 cut(s)	43
HphI	GGTGA	1 cut(s)	174
Hpy166II	GTNNAC	2 cut(s)	384, 658
Hpy188I	TCNGA	5 cut(s)	85, 108, 531, 934, 1012
Hpy188III	TCNNGA	5 cut(s)	434, 619, 739, 887, 911
Hpy8I	GTNNAC	2 cut(s)	384, 658
HpyAV	CCTTC	4 cut(s)	195, 466, 478, 736
HpyCH4III	ACNGT	1 cut(s)	794
HpyCH4IV	ACGT	1 cut(s)	665
HpyCH4V	TGCA	4 cut(s)	492, 560, 985, 1006
HpyF3I	CTNAG	6 cut(s)	201, 297, 335, 465, 513, 933
HpySE526I	ACGT	1 cut(s)	665
Hsp92II	CATG	6 cut(s)	95, 275, 307, 410, 642, 812
HspAI	GCGC	1 cut(s)	1016
KpnI	GGTACC	1 cut(s)	793
Ksp22I	TGATCA	1 cut(s)	436
Kzo9I	GATC	4 cut(s)	331, 436, 495, 544
LguI	GCTCTTC	1 cut(s)	934
LmnI	GCTCC	1 cut(s)	475
LweI	GCATC	9 cut(s)	3, 40, 403, 499, 606, 690, 972, 993, 1027
MaeI	CTAG	4 cut(s)	132, 153, 455, 459
MaeII	ACGT	1 cut(s)	665
MaeIII	GTNAC	2 cut(s)	424, 832
MalI	GATC	4 cut(s)	333, 438, 497, 546
MboI	GATC	4 cut(s)	331, 436, 495, 544
MboII	GAAGA	4 cut(s)	121, 481, 731, 921
MfeI	CAATTG	1 cut(s)	258
MhlI	GDGCHC	2 cut(s)	168, 894
MlsI	TGGCCA	1 cut(s)	580
MluCI	AATT	6 cut(s)	135, 258, 774, 916, 922, 1024
MluNI	TGGCCA	1 cut(s)	580
MmeI	TCCRAC	3 cut(s)	63, 787, 918
Mox20I	TGGCCA	1 cut(s)	580
Mph1103I	ATGCAT	1 cut(s)	987
MscI	TGGCCA	1 cut(s)	580
MseI	TTAA	2 cut(s)	402, 843
MslI	CAYNNNNRTG	1 cut(s)	411
Msp20I	TGGCCA	1 cut(s)	580
MspA1I	CMGCKG	1 cut(s)	329
MspI	CCGG	1 cut(s)	43
MspR9I	CCNGG	3 cut(s)	190, 208, 285
MunI	CAATTG	1 cut(s)	258
MvaI	CCWGG	3 cut(s)	190, 208, 285
NcoI	CCATGG	1 cut(s)	808
NdeII	GATC	4 cut(s)	331, 436, 495, 544
NheI	GCTAGC	1 cut(s)	454
NlaIII	CATG	6 cut(s)	95, 275, 307, 410, 642, 812
NlaIV	GGNNCC	3 cut(s)	25, 477, 791
NmeAIII	GCCGAG	1 cut(s)	52
NmuCI	GTSAC	1 cut(s)	424
NsiI	ATGCAT	1 cut(s)	987
NspI	RCATGY	2 cut(s)	410, 642
PasI	CCCWGGG	1 cut(s)	284
PceI	AGGCCT	1 cut(s)	193
PciSI	GCTCTTC	1 cut(s)	934
PfeI	GAWTC	3 cut(s)	750, 860, 941
PflFI	GACNNNGTC	1 cut(s)	272
PflMI	CCANNNNNTGG	1 cut(s)	593
PkrI	GCNGC	1 cut(s)	806
PsiI	TTATAA	1 cut(s)	921
Psp124BI	GAGCTC	1 cut(s)	894
Psp1406I	AACGTT	1 cut(s)	665
Psp6I	CCWGG	3 cut(s)	188, 206, 283
PspGI	CCWGG	3 cut(s)	188, 206, 283
PspN4I	GGNNCC	3 cut(s)	25, 477, 791
PspPI	GGNCC	3 cut(s)	24, 650, 779
PstI	CTGCAG	1 cut(s)	562
PstNI	CAGNNNCTG	1 cut(s)	106
PsyI	GACNNNGTC	1 cut(s)	272
PvuII	CAGCTG	1 cut(s)	329
RsaI	GTAC	5 cut(s)	376, 575, 659, 725, 791
RsaNI	GTAC	5 cut(s)	375, 574, 658, 724, 790
RseI	CAYNNNNRTG	1 cut(s)	411
SacI	GAGCTC	1 cut(s)	894
SapI	GCTCTTC	1 cut(s)	934
SaqAI	TTAA	2 cut(s)	402, 843
SatI	GCNGC	1 cut(s)	805
Sau3AI	GATC	4 cut(s)	331, 436, 495, 544
Sau96I	GGNCC	3 cut(s)	24, 650, 779
ScaI	AGTACT	1 cut(s)	575
ScrFI	CCNGG	3 cut(s)	190, 208, 285
SduI	GDGCHC	2 cut(s)	168, 894
SfaNI	GCATC	9 cut(s)	3, 40, 403, 499, 606, 690, 972, 993, 1027
SfcI	CTRYAG	1 cut(s)	558
SinI	GGWCC	1 cut(s)	650
SmiMI	CAYNNNNRTG	1 cut(s)	411
SmlI	CTYRAG	2 cut(s)	320, 962
SmoI	CTYRAG	2 cut(s)	320, 962
Sse9I	AATT	6 cut(s)	135, 258, 774, 916, 922, 1024
SseBI	AGGCCT	1 cut(s)	193
SsiI	CCGC	2 cut(s)	542, 805
SspMI	CTAG	4 cut(s)	132, 153, 455, 459
SstI	GAGCTC	1 cut(s)	894
StuI	AGGCCT	1 cut(s)	193
StyD4I	CCNGG	3 cut(s)	188, 206, 283
StyI	CCWWGG	3 cut(s)	390, 711, 808
TaaI	ACNGT	1 cut(s)	794
TaiI	ACGT	1 cut(s)	668
TaqI	TCGA	3 cut(s)	547, 738, 886
TasI	AATT	6 cut(s)	135, 258, 774, 916, 922, 1024
TatI	WGTACW	2 cut(s)	573, 723
TauI	GCSGC	1 cut(s)	807
TfiI	GAWTC	3 cut(s)	750, 860, 941
Tru1I	TTAA	2 cut(s)	402, 843
Tru9I	TTAA	2 cut(s)	402, 843
TseFI	GTSAC	1 cut(s)	424
Tsp45I	GTSAC	1 cut(s)	424
TspDTI	ATGAA	2 cut(s)	80, 202
Tth111I	GACNNNGTC	1 cut(s)	272
Van91I	CCANNNNNTGG	1 cut(s)	593
VpaK11BI	GGWCC	1 cut(s)	650
XapI	RAATTY	2 cut(s)	135, 1024
XceI	RCATGY	2 cut(s)	410, 642
XspI	CTAG	4 cut(s)	132, 153, 455, 459
ZrmI	AGTACT	1 cut(s)	575
Zsp2I	ATGCAT	1 cut(s)	987

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.