MD16G1202200.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. MPBQ MBSQ MT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002098
Physical Location & Seq
Reverse (-)
18389803 .. 18393081
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1202200.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCGATTTCGATGCTCTACGGAGCTCAGAATCTCAAGCTTGTCAATGGAATGGTAGCAGTAGCACCAAACGGGTTAGGTTCTGGTGGGAAAAATTACCCTTATGGGGGTTTTTCGCACAAGGGTTTAGTGAGCTATGGCTGCAGGAGTCAAAAGACCAGAACTTTAGTGGGACCCATATGCAGTGTGTCATCGTCAAGGCCTGCTTCCCAGCCTAGGTTCATCCAGCACAAGCAGGAGGCTTTCTGGTTCTATAGGTTTTTGTCTATAGTGTATGATCATGTTATAAACCCGGGGCATTGGACTGAGGATATGCGGGATGATGCGCTCGAGCCCGCGGATCTTTACGATAGGAATATGGTGGTGGTGGATGTTGGAGGTGGAACTGGGTTTACGACTCTTGGCATTGTTAAGCATGTGGATGCCAAGAATGTGACCATTCTCGACCAATCGCCGCACCAGCTTGCCAAGGCTAAGGAGAAGGAGCCATTGAAAGAATGTAAGATCATTGAAGGCGATGCGGAGGATCTGCCGTTCCCAACCGATTATGCAGATCGATATGTCTCTGCTGGAAGTATTGAGTACTGGCCCGACCCACAGCGTGGAATCAAGGAAGCATACAGGGTACTAAAAATAAGAGGAAAGGCTTGCCTCATAGGCCCTGTGTACCCAACCTTTTGGCTGTCTCGTTTCTTTTCGGATGTGTGGATGCTCTTCCCAAAGGAGGAGGAGTACATTGAGTGGTTCAAAAATGCTGGTTTCGAAGATGTTCAACTGAAAAGGATTGGTCCAAAATGGTACCGTGGTGTCCGCCGCCATGGCTTGATTATGGGTTGCTCTGTGACAGGTGTGAAGCCCTTAAGTGGGGACTCCCCTCTTCAGCTTGGTCCAAAGGTGGAGGACGTAAGGAAGCCTGTGAATCCCTTGGTGTTTCTTTTTCGGTTCATTCTCGGTGCAACTGCAGGAGCTTACTATGTGCTGGTTCCAATTTACATGTGGATTAAAGATCAAGTCGTTCCAAAAGGCCAGCCTATCTGA

Protein Analysis

346

Amino Acids

38.76

Weight (kDa)

8.98

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 117 - 219 6.5e-12 Methyltransferase domain
Ubie_methyltran PF01209 119 - 219 1.6e-10 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 122 - 214 2.3e-16 Methyltransferase domain
Methyltransf_11 PF08241 123 - 217 1e-18 Methyltransferase domain
Methyltransf_12 PF08242 123 - 215 1.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
Acc65I GGTACC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 798
AccB7I CCANNNNNTGG 1 cut(s) 602
AccII CGCG 1 cut(s) 338
AciI CCGC 7 cut(s) 316, 336, 338, 455, 521, 811, 814
AclWI GGATC 2 cut(s) 348, 534
AcuI CTGAAG 1 cut(s) 863
AdeI CACNNNGTG 1 cut(s) 602
AfaI GTAC 5 cut(s) 584, 627, 668, 734, 800
AfiI CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
AflII CTTAAG 1 cut(s) 859
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 4 cut(s) 493, 512, 748, 773
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 6 cut(s) 26, 40, 135, 463, 883, 968
AluI AGCT 6 cut(s) 26, 40, 135, 463, 883, 968
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 2 cut(s) 568, 690
AlwI GGATC 2 cut(s) 348, 534
Ama87I CYCGRG 2 cut(s) 292, 329
AoxI GGCC 4 cut(s) 200, 587, 658, 1024
ApeKI GCWGC 1 cut(s) 141
Asp700I GAANNNNTTC 1 cut(s) 768
Asp718I GGTACC 1 cut(s) 798
AspA2I CCTAGG 1 cut(s) 215
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 5 cut(s) 173, 588, 659, 788, 887
AsuC2I CCSGG 2 cut(s) 293, 294
AsuII TTCGAA 1 cut(s) 762
AvaI CYCGRG 2 cut(s) 292, 329
AvaII GGWCC 3 cut(s) 173, 788, 887
AvrII CCTAGG 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 790, 823
BanI GGYRCC 1 cut(s) 798
BanII GRGCYC 2 cut(s) 28, 336
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 517
BcgI CGANNNNNNTGC 1 cut(s) 29
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 2 cut(s) 293, 294
BcoDI GTCTC 2 cut(s) 568, 690
BfaI CTAG 1 cut(s) 216
BfmI CTRYAG 4 cut(s) 142, 253, 267, 960
BfrI CTTAAG 1 cut(s) 859
BglI GCCNNNNNGGC 2 cut(s) 657, 819
BisI GCNGC 3 cut(s) 142, 455, 814
BlnI CCTAGG 1 cut(s) 215
BlsI GCNGC 3 cut(s) 143, 456, 815
BmcAI AGTACT 1 cut(s) 584
Bme1390I CCNGG 2 cut(s) 293, 294
Bme18I GGWCC 3 cut(s) 173, 788, 887
BmeT110I CYCGRG 2 cut(s) 292, 329
BmgT120I GGNCC 5 cut(s) 173, 588, 659, 788, 887
BmiI GGNNCC 5 cut(s) 174, 175, 486, 800, 984
BmrFI CCNGG 2 cut(s) 293, 294
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 5 cut(s) 3, 313, 412, 508, 699
BmuI ACTGGG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 20
BpuMI CCSGG 2 cut(s) 293, 294
Bsa29I ATCGAT 1 cut(s) 556
BsaJI CCNNGG 8 cut(s) 215, 292, 293, 336, 468, 802, 817, 924
BsaXI ACNNNNNCTCC 2 cut(s) 716, 746
Bsc4I CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
Bse1I ACTGG 2 cut(s) 391, 590
BseCI ATCGAT 1 cut(s) 556
BseDI CCNNGG 8 cut(s) 215, 292, 293, 336, 468, 802, 817, 924
BseGI GGATG 6 cut(s) 222, 325, 376, 427, 706, 714
BseLI CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
BseMII CTCAG 2 cut(s) 41, 297
BseNI ACTGG 2 cut(s) 391, 590
BseRI GAGGAG 2 cut(s) 740, 743
BseXI GCAGC 1 cut(s) 128
BseYI CCCAGC 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 4 cut(s) 202, 589, 660, 1026
BshNI GGYRCC 1 cut(s) 798
BshVI ATCGAT 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 28
BsiHKCI CYCGRG 2 cut(s) 292, 329
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 2 cut(s) 186, 881
BslI CCNNNNNNNGG 7 cut(s) 106, 107, 216, 602, 724, 863, 864
BsmAI GTCTC 2 cut(s) 568, 690
BsmFI GGGAC 2 cut(s) 186, 881
BsnI GGCC 4 cut(s) 202, 589, 660, 1026
BsoBI CYCGRG 2 cut(s) 292, 329
Bsp119I TTCGAA 1 cut(s) 762
Bsp1286I GDGCHC 2 cut(s) 28, 336
Bsp143I GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
Bsp19I CCATGG 1 cut(s) 817
BspACI CCGC 7 cut(s) 316, 336, 338, 455, 521, 811, 814
BspANI GGCC 4 cut(s) 202, 589, 660, 1026
BspCNI CTCAG 2 cut(s) 40, 298
BspDI ATCGAT 1 cut(s) 556
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 5 cut(s) 174, 175, 486, 800, 984
BspMAI CTGCAG 2 cut(s) 146, 964
BspPI GGATC 2 cut(s) 348, 534
BspQI GCTCTTC 1 cut(s) 719
BspT104I TTCGAA 1 cut(s) 762
BspT107I GGYRCC 1 cut(s) 798
BspTI CTTAAG 1 cut(s) 859
BsrI ACTGG 2 cut(s) 391, 590
BssECI CCNNGG 8 cut(s) 215, 292, 293, 336, 468, 802, 817, 924
BssMI GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
BssT1I CCWWGG 4 cut(s) 215, 468, 817, 924
Bst4CI ACNGT 1 cut(s) 803
Bst6I CTCTTC 2 cut(s) 719, 882
BstAFI CTTAAG 1 cut(s) 859
BstBI TTCGAA 1 cut(s) 762
BstC8I GCNNGC 5 cut(s) 204, 336, 465, 649, 1028
BstDEI CTNAG 3 cut(s) 27, 306, 474
BstDSI CCRYGG 3 cut(s) 336, 802, 817
BstF5I GGATG 6 cut(s) 222, 325, 376, 427, 706, 714
BstFNI CGCG 1 cut(s) 338
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 6 cut(s) 280, 343, 507, 529, 556, 1009
BstMAI GTCTC 2 cut(s) 568, 690
BstMBI GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
BstMWI GCNNNNNNNGC 4 cut(s) 141, 460, 657, 819
BstNSI RCATGY 2 cut(s) 419, 997
BstSCI CCNGG 2 cut(s) 291, 292
BstSFI CTRYAG 4 cut(s) 142, 253, 267, 960
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 2 cut(s) 340, 526
BstXI CCANNNNNNTGG 1 cut(s) 678
BstYI RGATCY 2 cut(s) 340, 526
Bsu15I ATCGAT 1 cut(s) 556
BsuRI GGCC 4 cut(s) 202, 589, 660, 1026
BsuTUI ATCGAT 1 cut(s) 556
BtgI CCRYGG 3 cut(s) 336, 802, 817
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 6 cut(s) 222, 325, 376, 427, 706, 714
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 190
Cac8I GCNNGC 5 cut(s) 204, 336, 465, 649, 1028
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 5 cut(s) 173, 588, 659, 788, 887
Cfr42I CCGCGG 1 cut(s) 339
Cfr9I CCCGGG 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 556
Csp6I GTAC 5 cut(s) 583, 626, 667, 733, 799
CviAII CATG 4 cut(s) 281, 416, 818, 994
CviQI GTAC 5 cut(s) 583, 626, 667, 733, 799
DdeI CTNAG 3 cut(s) 27, 306, 474
DpnI GATC 6 cut(s) 279, 342, 506, 528, 555, 1008
DpnII GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
DraIII CACNNNGTG 1 cut(s) 602
Eam1104I CTCTTC 2 cut(s) 719, 882
EarI CTCTTC 2 cut(s) 719, 882
EciI GGCGGA 1 cut(s) 800
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 4 cut(s) 215, 468, 817, 924
Eco147I AGGCCT 1 cut(s) 202
Eco24I GRGCYC 2 cut(s) 28, 336
Eco47I GGWCC 3 cut(s) 173, 788, 887
Eco53kI GAGCTC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 863
Eco88I CYCGRG 2 cut(s) 292, 329
EcoICRI GAGCTC 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 173, 659
EcoT14I CCWWGG 4 cut(s) 215, 468, 817, 924
EcoT38I GRGCYC 2 cut(s) 28, 336
ErhI CCWWGG 4 cut(s) 215, 468, 817, 924
FaeI CATG 4 cut(s) 284, 419, 821, 997
FalI AAGNNNNNCTT 2 cut(s) 190, 222
FaqI GGGAC 2 cut(s) 186, 881
FatI CATG 4 cut(s) 280, 415, 817, 993
FauI CCCGC 2 cut(s) 309, 343
FauNDI CATATG 1 cut(s) 179
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 142, 455, 814
FokI GGATG 6 cut(s) 209, 332, 383, 434, 713, 721
FriOI GRGCYC 2 cut(s) 28, 336
Fsp4HI GCNGC 3 cut(s) 142, 455, 814
FspBI CTAG 1 cut(s) 216
GlaI GCGC 1 cut(s) 327
GluI GCNGC 3 cut(s) 142, 455, 814
GsaI CCCAGC 1 cut(s) 214
HaeIII GGCC 4 cut(s) 202, 589, 660, 1026
HapII CCGG 1 cut(s) 293
HhaI GCGC 1 cut(s) 328
Hin1II CATG 4 cut(s) 284, 419, 821, 997
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 6 cut(s) 31, 148, 397, 606, 869, 919
HpaII CCGG 1 cut(s) 293
Hpy166II GTNNAC 2 cut(s) 393, 667
Hpy188I TCNGA 3 cut(s) 30, 700, 1037
Hpy188III TCNNGA 1 cut(s) 443
Hpy8I GTNNAC 2 cut(s) 393, 667
HpyAV CCTTC 2 cut(s) 475, 506
HpyCH4III ACNGT 1 cut(s) 803
HpyCH4IV ACGT 1 cut(s) 903
HpyCH4V TGCA 5 cut(s) 144, 183, 551, 956, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 460, 657, 819
HpyF3I CTNAG 3 cut(s) 27, 306, 474
HpySE526I ACGT 1 cut(s) 903
Hsp92II CATG 4 cut(s) 284, 419, 821, 997
HspAI GCGC 1 cut(s) 326
KflI GGGWCCC 1 cut(s) 173
KpnI GGTACC 1 cut(s) 802
Ksp22I TGATCA 1 cut(s) 277
KspI CCGCGG 1 cut(s) 339
Kzo9I GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 3 cut(s) 23, 484, 965
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 5 cut(s) 3, 313, 412, 508, 699
MaeI CTAG 1 cut(s) 216
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 2 cut(s) 433, 841
MalI GATC 6 cut(s) 279, 342, 506, 528, 555, 1008
MboI GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
MboII GAAGA 3 cut(s) 706, 776, 869
MflI RGATCY 2 cut(s) 340, 526
MhlI GDGCHC 2 cut(s) 28, 336
MluCI AATT 2 cut(s) 94, 987
MlyI GAGTC 3 cut(s) 157, 391, 863
MmeI TCCRAC 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 3 cut(s) 411, 860, 1002
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 338
MspCI CTTAAG 1 cut(s) 859
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 2 cut(s) 293, 294
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 141, 460, 657, 819
NciI CCSGG 2 cut(s) 293, 294
NcoI CCATGG 1 cut(s) 817
NdeI CATATG 1 cut(s) 179
NdeII GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
NlaIII CATG 4 cut(s) 284, 419, 821, 997
NlaIV GGNNCC 5 cut(s) 174, 175, 486, 800, 984
NmuCI GTSAC 2 cut(s) 433, 841
NspI RCATGY 2 cut(s) 419, 997
NspV TTCGAA 1 cut(s) 762
PaeR7I CTCGAG 1 cut(s) 329
PceI AGGCCT 1 cut(s) 202
PciI ACATGT 1 cut(s) 993
PciSI GCTCTTC 1 cut(s) 719
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 3 cut(s) 31, 606, 919
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 3 cut(s) 143, 456, 815
PleI GAGTC 3 cut(s) 156, 391, 863
PpsI GAGTC 3 cut(s) 156, 391, 863
PpuMI RGGWCCY 1 cut(s) 173
PscI ACATGT 1 cut(s) 993
PsiI TTATAA 1 cut(s) 287
Psp124BI GAGCTC 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 210
PspN4I GGNNCC 5 cut(s) 174, 175, 486, 800, 984
PspPI GGNCC 5 cut(s) 173, 588, 659, 788, 887
PspPPI RGGWCCY 1 cut(s) 173
PspXI VCTCGAGB 1 cut(s) 329
PsrI GAACNNNNNNTAC 2 cut(s) 376, 408
PstI CTGCAG 2 cut(s) 146, 964
PsuI RGATCY 2 cut(s) 340, 526
RsaI GTAC 5 cut(s) 584, 627, 668, 734, 800
RsaNI GTAC 5 cut(s) 583, 626, 667, 733, 799
RseI CAYNNNNRTG 1 cut(s) 420
SacI GAGCTC 1 cut(s) 28
SacII CCGCGG 1 cut(s) 339
SapI GCTCTTC 1 cut(s) 719
SaqAI TTAA 3 cut(s) 411, 860, 1002
SatI GCNGC 3 cut(s) 142, 455, 814
Sau3AI GATC 6 cut(s) 277, 340, 504, 526, 553, 1006
Sau96I GGNCC 5 cut(s) 173, 588, 659, 788, 887
ScaI AGTACT 1 cut(s) 584
SchI GAGTC 3 cut(s) 157, 391, 863
ScrFI CCNGG 2 cut(s) 293, 294
SduI GDGCHC 2 cut(s) 28, 336
SfaNI GCATC 5 cut(s) 3, 313, 412, 508, 699
SfcI CTRYAG 4 cut(s) 142, 253, 267, 960
Sfr274I CTCGAG 1 cut(s) 329
Sfr303I CCGCGG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 762
SgrBI CCGCGG 1 cut(s) 339
SinI GGWCC 3 cut(s) 173, 788, 887
SlaI CTCGAG 1 cut(s) 329
SmaI CCCGGG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 3 cut(s) 35, 329, 859
SmoI CTYRAG 3 cut(s) 35, 329, 859
Sse9I AATT 2 cut(s) 94, 987
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 7 cut(s) 316, 336, 338, 455, 521, 811, 814
SspMI CTAG 1 cut(s) 216
SstI GAGCTC 1 cut(s) 28
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 291, 292
StyI CCWWGG 4 cut(s) 215, 468, 817, 924
TaaI ACNGT 1 cut(s) 803
TaiI ACGT 1 cut(s) 906
TaqI TCGA 5 cut(s) 11, 330, 444, 556, 762
TasI AATT 2 cut(s) 94, 987
TatI WGTACW 2 cut(s) 582, 732
TauI GCSGC 2 cut(s) 457, 816
TfiI GAWTC 3 cut(s) 31, 606, 919
Tru1I TTAA 3 cut(s) 411, 860, 1002
Tru9I TTAA 3 cut(s) 411, 860, 1002
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 2 cut(s) 433, 841
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 2 cut(s) 433, 841
TspDTI ATGAA 2 cut(s) 211, 934
TspGWI ACGGA 1 cut(s) 36
TspMI CCCGGG 1 cut(s) 292
TspRI CASTG 1 cut(s) 190
Van91I CCANNNNNTGG 1 cut(s) 602
Vha464I CTTAAG 1 cut(s) 859
VpaK11BI GGWCC 3 cut(s) 173, 788, 887
XceI RCATGY 2 cut(s) 419, 997
XcmI CCANNNNNNNNNTGG 2 cut(s) 166, 993
XhoI CTCGAG 1 cut(s) 329
XmaI CCCGGG 1 cut(s) 292
XmaJI CCTAGG 1 cut(s) 215
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 1 cut(s) 216
ZrmI AGTACT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.