Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0001261

Physical Location & Seq

Genomic Coordinates Forward (+)

2057274 .. 2066098

Gene Structure 8825 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g02230.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGCCCTGCCAGTTTTGGTGTATCAAATCCTCATCTTTACATGGACGTAGTTTCAAATCGCCAAACAGAGGAAATTGCAAGGTCATGTCTCAAAAGACTGGCAATAAATTAGATTCCACTATGTACATAAATGTTGAAGATGACTCGGACATTGAACTCCTTAGGATAGAACAAGTAAAAAGTTCACAGATTCACCTAGCTAAAGTCTTGATGGGATTGACATGCTTATATATTCCACTCAGACTGGCAAAGCTAAATGTACTTGATATTTTCATTAAGATTGCCCAAGAAAGATTTCTCTGTACTGTTCAAAATATTGGGGCTGAGACCTTGCCTTTTGCCTGTGCTTCTAATTCAATGAATAAACCCCTGCCTCTTGGATTGGATGTGACGTTGCCATCATTCCAAGATGTTAAATGGAGCTTTCACAGATTATTGTATCTTTTCAACGTCCAACTTGAGAAAAATGTTGCCACGTTCTTCATAGTGCTCCTGGTAGCATGTTTCTCATTTGTTCTCATTGGTGGTTTCCTGTTCTACAAGTTTAGGGATAGTAAAGAATCTTTGGAGGACTGCTTCTGGGAAGCTTGGGCATGCCTCTGTTCATCATCTACACATCTAAAGCAAAGAACACGTATTGAACGTGTTATTGGTTTCGTTCTTGCTATATGGGGCATACTATTTTACACCCGCCTATTGAGCACAATGACTGAACAGTTCCGGAGTAATATGTACAAGCTCAGAGAAGGGGCACAAATGCAAGTTCTAGAGACTGATCACATCATTATTTGTGGAGTGAACAGCCATCTTGCTTTCATACTAAAGCAATTAAACAAGTATCATGAATTTGCTGTCCGTTTAGGTACTGCTACAGCTAGGAAGCAAAGAATTCTTCTTATGTCTGATCTGCCAAGAAAGCAGATAGACAAGTTAGCTGATCAGATTGCCAAAGATTTTAACCATATCGATATCCTTACAAAAAGTTGTAGCCTGAGCTTAACAAAATCATATGAAAGAGCTGCTGCCAACAAGGCACGTGCCATAATTATACTGCCTACAAAGACAGATCGGTATGAAGTTGACACTGATGCCTTTCTATCTGTTCTGGCTCTTCAACCTATTCCTAATATGGAATCCGTCCCCACTATAGTTGAGGTTTCAAGTTCCAATACATGTGAGCTTTTAAAGTCAATCTCAGGGTTGAAAGTAGAGCCTGTGGAGAACGTTGCATCCAAATTATTTGTTCAATGCTCTCGTCAAAAAGGACTAATAAAGATCTACAGACACTTGCTTAACTATCGAAAAAATGTGTTCAACCTTTGGAATTCTCCTAGTCTTGCAGGGCTGAAGTATAGAGAATTAGGGCCTGGGTTTCAAGAGGCAGTTGTTTGTGGGCTTTATAGAAATGAGAAGATATACTTCCACCCAAATGATGATGAGATCTTGCAGGAAACTGACAAAGTATTGTTCATTGCACCTGTCAATGGGGCTAAGAAACCTGCTATTACTTATTCAAATGTTAAAGAAATAAGTGATGCCAATCGAAGTGTTGAAGATGTAGAAAAGGACAGTGATACTCAATCGTATGCCTTAAAAATAAGGAAAGAAAACACACGTCCCAAGAAAAGAGGCTCCAAGGCTTCAGATGGGACTCTAGGACCAAAAGAATTTATACTTCTTCTTGGATGGCGGCCAGACGTTGTTGAGATGATTGAAGAGTATGACAATTATCTCGGACCTGGGAGTGTTGTGGAGATATTATCAGATGTACCATTAGATGACAGAAATAAGGCAACAAAAGGTGCCGGCGGTCAAGCCAAACTAAAAAATGTGCAGGTTTCACACAGGATCGGAAACCCTATGAACTTTGACACTTTACAAGAAACCATACGCAATATCCAACTTTCTTCGAAAAATGAAGATATTCCCTTATCCATTGTTGTAATATCTGATAGAGAATGGCTTCTTGGAGATCCAAACAGGGCAGACAAGCAATCCGCCTATTCCCTTCTTCTTGCTGAAAATATTTGCAACAAACTTGGTGTAACGGTGCAAAATCTAGTTGCAGAGATTGTGGACTCGAAATTGGGGAAACAAATCACAAGAATAAAGCCATCCCTGACTTATATTGCCGCGGAGGAAGTAATGAGCCTCGTAACAGCTCAAGTTGCTGAGGACAGTGAACTAAATGAGGTGTGGAAAGACATTCTGAATGCAGAAGGTGATGAAATATATGTGAAGGATATCAGACTCTATATGAAGGAAGGAGAGATTCCATCGTTCACAGAGCTTGCTAAAAGAGCACATTTACGGAGAGAAGTGGCCATAGGTTATGTGAAAAACAATAAGAAGGTTATTAATCCAGTTCCCAAGTCAGAACCCCTCTCCCTTGAGTTGACAGATTCGTTAATAGTTATATCTGAACTTGAAGGAGAACAACCAATCCTTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Castor_Poll_mid

Protein Analysis

818

Amino Acids

92.31

Weight (kDa)

8.27

Isoelectric Point (pI)

43.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Castor_Poll_mid	PF06241	439 - 539	9.8e-38	Castor and Pollux, part of voltage-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001261)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02940 AT5G02940 AT5G43745
fragaria_vesca	FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230
malus_domestica	MD11G1069300.v1.1 MD13G1204000.v1.1 MD16G1206000.v1.1
prunus_persica	Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1
pyrus_communis	pycom11g05780 pycom13g17720
rosa_chinensis	RchiOBHm_Chr4g0389551 RchiOBHm_Chr4g0389561 RchiOBHm_Chr4g0389571 RchiOBHm_Chr4g0389581
rosa_laevigata	RLG00000009970 RLG00000009971 RLG00000009972
rosa_multiflora	Rmu_co8238337.1_g000001 Rmu_co8398985.1_g000001 Rmu_sc0010540.1_g000001 Rmu_ssc0000478.1_g000001 Rmu_ssc0000478.1_g000002 Rmu_ssc0000478.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335510 Rroxscaffold_5G00335520 Rroxscaffold_5G00335530
rosa_rugosa	Rorug03G0324000
rosa_samantha	Rh2BG306500 Rh2CG284600 Rh4AG028300 Rh4AG028400 Rh4AG028500 Rh4BG021500 Rh4BG021600 Rh4BG021700 Rh4CG030500 Rh4CG030700 Rh4DG022100 Rh4DG022200 Rh4DG022300
rosa_wichuraiana	Rw4G002110 Rw4G002120 Rw4G002130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1507, 1825
AccB1I	GGYRCC	1 cut(s)	1802
AccII	CGCG	1 cut(s)	2135
AccIII	TCCGGA	1 cut(s)	720
AciI	CCGC	6 cut(s)	691, 1690, 1809, 1998, 2133, 2135
AclI	AACGTT	1 cut(s)	1224
AclWI	GGATC	2 cut(s)	1856, 1967
AcoI	YGGCCR	2 cut(s)	1691, 2322
AcsI	RAATTY	4 cut(s)	845, 888, 1324, 1667
AcuI	CTGAAG	2 cut(s)	1367, 1626
AcvI	CACGTG	1 cut(s)	1037
AfaI	GTAC	6 cut(s)	125, 261, 304, 734, 865, 1770
AfiI	CCNNNNNNNGG	2 cut(s)	68, 1484
AflIII	ACRYGT	4 cut(s)	632, 643, 1172, 1613
AjiI	CACGTC	1 cut(s)	1616
AjnI	CCWGG	3 cut(s)	492, 1366, 1738
AjuI	GAANNNNNNNTTGG	6 cut(s)	279, 311, 633, 665, 1950, 1982
AloI	GAACNNNNNNTCC	3 cut(s)	1214, 1246, 2427
Alw21I	GWGCWC	3 cut(s)	492, 704, 2305
Alw26I	GTCTC	3 cut(s)	93, 320, 764
AlwI	GGATC	2 cut(s)	1856, 1967
Aor13HI	TCCGGA	1 cut(s)	720
AoxI	GGCC	3 cut(s)	1364, 1691, 2322
ApeKI	GCWGC	2 cut(s)	1019, 1022
ApoI	RAATTY	4 cut(s)	845, 888, 1324, 1667
AseI	ATTAAT	1 cut(s)	2358
Asp700I	GAANNNNTTC	3 cut(s)	294, 1923, 1962
AspS9I	GGNCC	3 cut(s)	1364, 1658, 1736
AsuHPI	GGTGA	2 cut(s)	185, 2234
AsuII	TTCGAA	1 cut(s)	1910
AvaII	GGWCC	2 cut(s)	1658, 1736
AxyI	CCTNAGG	1 cut(s)	161
BaeGI	GKGCMC	1 cut(s)	754
BalI	TGGCCA	1 cut(s)	2324
BanI	GGYRCC	1 cut(s)	1802
BbrPI	CACGTG	1 cut(s)	1037
Bbv12I	GWGCWC	3 cut(s)	492, 704, 2305
BbvCI	CCTCAGC	1 cut(s)	2172
BbvI	GCAGC	2 cut(s)	1006, 1009
BccI	CCATC	7 cut(s)	205, 406, 813, 1640, 1680, 2122, 2284
BciT130I	CCWGG	3 cut(s)	494, 1368, 1740
BclI	TGATCA	2 cut(s)	775, 937
BcoDI	GTCTC	3 cut(s)	93, 320, 764
BfaI	CTAG	6 cut(s)	197, 767, 876, 1332, 1655, 2060
BfmI	CTRYAG	3 cut(s)	870, 1146, 1279
BfuAI	ACCTGC	2 cut(s)	1507, 1825
BglI	GCCNNNNNGGC	1 cut(s)	1031
BglII	AGATCT	2 cut(s)	1275, 1440
BisI	GCNGC	4 cut(s)	1020, 1023, 1691, 2133
BlsI	GCNGC	4 cut(s)	1021, 1024, 1692, 2134
Bme1390I	CCNGG	3 cut(s)	494, 1368, 1740
Bme18I	GGWCC	2 cut(s)	1658, 1736
BmgBI	CACGTC	1 cut(s)	1616
BmgT120I	GGNCC	3 cut(s)	1364, 1658, 1736
BmiI	GGNNCC	2 cut(s)	1633, 1804
BmrFI	CCNGG	3 cut(s)	494, 1368, 1740
BmsI	GCATC	3 cut(s)	1078, 1238, 1525
Bpu10I	CCTNAGC	2 cut(s)	992, 2172
Bpu14I	TTCGAA	1 cut(s)	1910
BpuEI	CTTGAG	3 cut(s)	479, 2148, 2411
Bsa29I	ATCGAT	1 cut(s)	966
BsaAI	YACGTR	2 cut(s)	635, 1037
BsaBI	GATNNNNATC	1 cut(s)	1539
BsaI	GGTCTC	1 cut(s)	320
BsaJI	CCNNGG	4 cut(s)	1367, 1635, 1739, 2133
BsaWI	WCCGGW	1 cut(s)	720
Bsc4I	CCNNNNNNNGG	2 cut(s)	68, 1484
Bse118I	RCCGGY	1 cut(s)	1805
Bse1I	ACTGG	4 cut(s)	10, 103, 249, 2363
Bse21I	CCTNAGG	1 cut(s)	161
Bse3DI	GCAATG	1 cut(s)	1470
Bse8I	GATNNNNATC	1 cut(s)	1539
BseAI	TCCGGA	1 cut(s)	720
BseBI	CCWGG	3 cut(s)	494, 1368, 1740
BseCI	ATCGAT	1 cut(s)	966
BseDI	CCNNGG	4 cut(s)	1367, 1635, 1739, 2133
BseGI	GGATG	4 cut(s)	391, 1229, 1691, 2114
BseJI	GATNNNNATC	1 cut(s)	1539
BseLI	CCNNNNNNNGG	2 cut(s)	68, 1484
BseMI	GCAATG	1 cut(s)	1470
BseMII	CTCAG	6 cut(s)	253, 315, 754, 983, 1209, 2163
BseNI	ACTGG	4 cut(s)	10, 103, 249, 2363
BseSI	GKGCMC	1 cut(s)	754
BseXI	GCAGC	2 cut(s)	1006, 1009
BsgI	GTGCAG	1 cut(s)	1853
Bsh1236I	CGCG	1 cut(s)	2135
BshFI	GGCC	3 cut(s)	1366, 1693, 2324
BshNI	GGYRCC	1 cut(s)	1802
BshVI	ATCGAT	1 cut(s)	966
BsiHKAI	GWGCWC	3 cut(s)	492, 704, 2305
BsiSI	CCGG	2 cut(s)	721, 1806
BslFI	GGGAC	3 cut(s)	1124, 1602, 1663
BslI	CCNNNNNNNGG	2 cut(s)	68, 1484
BsmAI	GTCTC	3 cut(s)	93, 320, 764
BsmFI	GGGAC	3 cut(s)	1124, 1602, 1663
BsmI	GAATGC	1 cut(s)	2218
BsnI	GGCC	3 cut(s)	1366, 1693, 2324
Bso31I	GGTCTC	1 cut(s)	320
Bsp119I	TTCGAA	1 cut(s)	1910
Bsp1286I	GDGCHC	4 cut(s)	492, 704, 754, 2305
Bsp13I	TCCGGA	1 cut(s)	720
Bsp1407I	TGTACA	2 cut(s)	123, 732
Bsp143I	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
BspACI	CCGC	6 cut(s)	691, 1690, 1809, 1998, 2133, 2135
BspANI	GGCC	3 cut(s)	1366, 1693, 2324
BspCNI	CTCAG	6 cut(s)	252, 316, 753, 984, 1208, 2164
BspDI	ATCGAT	1 cut(s)	966
BspEI	TCCGGA	1 cut(s)	720
BspFNI	CGCG	1 cut(s)	2135
BspHI	TCATGA	1 cut(s)	841
BspLI	GGNNCC	2 cut(s)	1633, 1804
BspMI	ACCTGC	2 cut(s)	1507, 1825
BspPI	GGATC	2 cut(s)	1856, 1967
BspQI	GCTCTTC	1 cut(s)	1116
BspT104I	TTCGAA	1 cut(s)	1910
BspT107I	GGYRCC	1 cut(s)	1802
BspTNI	GGTCTC	1 cut(s)	320
BsrDI	GCAATG	1 cut(s)	1470
BsrFI	RCCGGY	1 cut(s)	1805
BsrGI	TGTACA	2 cut(s)	123, 732
BsrI	ACTGG	4 cut(s)	10, 103, 249, 2363
BssAI	RCCGGY	1 cut(s)	1805
BssECI	CCNNGG	4 cut(s)	1367, 1635, 1739, 2133
BssMI	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
BssT1I	CCWWGG	1 cut(s)	1635
Bst2UI	CCWGG	3 cut(s)	494, 1368, 1740
Bst4CI	ACNGT	5 cut(s)	307, 717, 1571, 2050, 2180
Bst6I	CTCTTC	2 cut(s)	1116, 1710
BstAUI	TGTACA	2 cut(s)	123, 732
BstBAI	YACGTR	2 cut(s)	635, 1037
BstBI	TTCGAA	1 cut(s)	1910
BstC8I	GCNNGC	3 cut(s)	595, 1807, 2292
BstDEI	CTNAG	8 cut(s)	161, 239, 324, 740, 992, 1195, 1491, 2172
BstDSI	CCRYGG	1 cut(s)	2133
BstF5I	GGATG	4 cut(s)	391, 1229, 1691, 2114
BstFNI	CGCG	1 cut(s)	2135
BstKTI	GATC	8 cut(s)	778, 907, 940, 1069, 1278, 1443, 1851, 1975
BstMAI	GTCTC	3 cut(s)	93, 320, 764
BstMBI	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
BstMWI	GCNNNNNNNGC	5 cut(s)	699, 916, 1031, 2168, 2300
BstNI	CCWGG	3 cut(s)	494, 1368, 1740
BstNSI	RCATGY	4 cut(s)	225, 504, 597, 1176
BstSCI	CCNGG	3 cut(s)	492, 1366, 1738
BstSFI	CTRYAG	3 cut(s)	870, 1146, 1279
BstSLI	GKGCMC	1 cut(s)	754
BstUI	CGCG	1 cut(s)	2135
BstV1I	GCAGC	2 cut(s)	1006, 1009
BstX2I	RGATCY	3 cut(s)	1275, 1440, 1972
BstYI	RGATCY	3 cut(s)	1275, 1440, 1972
Bsu15I	ATCGAT	1 cut(s)	966
Bsu36I	CCTNAGG	1 cut(s)	161
BsuRI	GGCC	3 cut(s)	1366, 1693, 2324
BsuTUI	ATCGAT	1 cut(s)	966
BtgI	CCRYGG	1 cut(s)	2133
BtrI	CACGTC	1 cut(s)	1616
BtsCI	GGATG	4 cut(s)	391, 1229, 1691, 2114
BtsIMutI	CAGTG	3 cut(s)	1083, 1576, 2185
BveI	ACCTGC	2 cut(s)	1507, 1825
Cac8I	GCNNGC	3 cut(s)	595, 1807, 2292
CciI	TCATGA	1 cut(s)	841
Cfr10I	RCCGGY	1 cut(s)	1805
Cfr13I	GGNCC	3 cut(s)	1364, 1658, 1736
Cfr42I	CCGCGG	1 cut(s)	2136
ClaI	ATCGAT	1 cut(s)	966
Csp6I	GTAC	6 cut(s)	124, 260, 303, 733, 864, 1769
CspCI	CAANNNNNGTGG	2 cut(s)	1132, 1167
CviAII	CATG	7 cut(s)	41, 85, 222, 501, 594, 842, 1173
CviQI	GTAC	6 cut(s)	124, 260, 303, 733, 864, 1769
DdeI	CTNAG	8 cut(s)	161, 239, 324, 740, 992, 1195, 1491, 2172
DpnI	GATC	8 cut(s)	777, 906, 939, 1068, 1277, 1442, 1850, 1974
DpnII	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
DraI	TTTAAA	1 cut(s)	1185
EaeI	YGGCCR	2 cut(s)	1691, 2322
Eam1104I	CTCTTC	2 cut(s)	1116, 1710
EarI	CTCTTC	2 cut(s)	1116, 1710
EciI	GGCGGA	1 cut(s)	1987
Eco130I	CCWWGG	1 cut(s)	1635
Eco31I	GGTCTC	1 cut(s)	320
Eco32I	GATATC	2 cut(s)	970, 2245
Eco47I	GGWCC	2 cut(s)	1658, 1736
Eco57I	CTGAAG	2 cut(s)	1367, 1626
Eco72I	CACGTG	1 cut(s)	1037
Eco81I	CCTNAGG	1 cut(s)	161
EcoO109I	RGGNCCY	1 cut(s)	1364
EcoRI	GAATTC	2 cut(s)	888, 1324
EcoRII	CCWGG	3 cut(s)	492, 1366, 1738
EcoRV	GATATC	2 cut(s)	970, 2245
EcoT14I	CCWWGG	1 cut(s)	1635
ErhI	CCWWGG	1 cut(s)	1635
FaeI	CATG	7 cut(s)	44, 88, 225, 504, 597, 845, 1176
FalI	AAGNNNNNCTT	2 cut(s)	1403, 1435
FaqI	GGGAC	3 cut(s)	1124, 1602, 1663
FatI	CATG	7 cut(s)	40, 84, 221, 500, 593, 841, 1172
FauI	CCCGC	1 cut(s)	698
FauNDI	CATATG	1 cut(s)	1009
FbaI	TGATCA	2 cut(s)	775, 937
Fnu4HI	GCNGC	4 cut(s)	1020, 1023, 1691, 2133
FokI	GGATG	4 cut(s)	398, 1216, 1698, 2101
Fsp4HI	GCNGC	4 cut(s)	1020, 1023, 1691, 2133
FspBI	CTAG	6 cut(s)	197, 767, 876, 1332, 1655, 2060
GluI	GCNGC	4 cut(s)	1020, 1023, 1691, 2133
HaeIII	GGCC	3 cut(s)	1366, 1693, 2324
HapII	CCGG	2 cut(s)	721, 1806
Hin1II	CATG	7 cut(s)	44, 88, 225, 504, 597, 845, 1176
HincII	GTYRAC	2 cut(s)	1081, 2397
HindII	GTYRAC	2 cut(s)	1081, 2397
HindIII	AAGCTT	1 cut(s)	585
HpaII	CCGG	2 cut(s)	721, 1806
HphI	GGTGA	2 cut(s)	185, 2234
Hpy166II	GTNNAC	7 cut(s)	185, 799, 1081, 2077, 2183, 2283, 2397
Hpy188III	TCNNGA	5 cut(s)	208, 721, 767, 842, 1376
Hpy8I	GTNNAC	7 cut(s)	185, 799, 1081, 2077, 2183, 2283, 2397
HpyAV	CCTTC	8 cut(s)	740, 2018, 2213, 2233, 2254, 2258, 2344, 2423
HpyCH4III	ACNGT	5 cut(s)	307, 717, 1571, 2050, 2180
HpyF10VI	GCNNNNNNNGC	5 cut(s)	699, 916, 1031, 2168, 2300
HpyF3I	CTNAG	8 cut(s)	161, 239, 324, 740, 992, 1195, 1491, 2172
Hsp92II	CATG	7 cut(s)	44, 88, 225, 504, 597, 845, 1176
Kpn2I	TCCGGA	1 cut(s)	720
KroI	GCCGGC	1 cut(s)	1805
KroNI	GCCGGC	1 cut(s)	1807
Ksp22I	TGATCA	2 cut(s)	775, 937
KspI	CCGCGG	1 cut(s)	2136
Kzo9I	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
LguI	GCTCTTC	1 cut(s)	1116
LmnI	GCTCC	3 cut(s)	420, 495, 1637
Lsp1109I	GCAGC	2 cut(s)	1006, 1009
LweI	GCATC	3 cut(s)	1078, 1238, 1525
MaeI	CTAG	6 cut(s)	197, 767, 876, 1332, 1655, 2060
MaeIII	GTNAC	3 cut(s)	388, 2044, 2155
MalI	GATC	8 cut(s)	777, 906, 939, 1068, 1277, 1442, 1850, 1974
MboI	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
MflI	RGATCY	3 cut(s)	1275, 1440, 1972
MhlI	GDGCHC	4 cut(s)	492, 704, 754, 2305
MlsI	TGGCCA	1 cut(s)	2324
MluNI	TGGCCA	1 cut(s)	2324
MlyI	GAGTC	4 cut(s)	137, 1645, 2072, 2244
MmeI	TCCRAC	2 cut(s)	478, 1924
Mox20I	TGGCCA	1 cut(s)	2324
MroI	TCCGGA	1 cut(s)	720
MroNI	GCCGGC	1 cut(s)	1805
MroXI	GAANNNNTTC	3 cut(s)	294, 1923, 1962
MscI	TGGCCA	1 cut(s)	2324
MslI	CAYNNNNRTG	1 cut(s)	1428
Msp20I	TGGCCA	1 cut(s)	2324
MspA1I	CMGCKG	1 cut(s)	2135
MspI	CCGG	2 cut(s)	721, 1806
MspR9I	CCNGG	3 cut(s)	494, 1368, 1740
Mva1269I	GAATGC	1 cut(s)	2218
MvaI	CCWGG	3 cut(s)	494, 1368, 1740
MvnI	CGCG	1 cut(s)	2135
MwoI	GCNNNNNNNGC	5 cut(s)	699, 916, 1031, 2168, 2300
NaeI	GCCGGC	1 cut(s)	1807
NdeI	CATATG	1 cut(s)	1009
NdeII	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
NgoMIV	GCCGGC	1 cut(s)	1805
NlaIII	CATG	7 cut(s)	44, 88, 225, 504, 597, 845, 1176
NlaIV	GGNNCC	2 cut(s)	1633, 1804
NmuCI	GTSAC	1 cut(s)	388
NspI	RCATGY	4 cut(s)	225, 504, 597, 1176
NspV	TTCGAA	1 cut(s)	1910
PaeI	GCATGC	1 cut(s)	597
PagI	TCATGA	1 cut(s)	841
PciI	ACATGT	1 cut(s)	1172
PciSI	GCTCTTC	1 cut(s)	1116
PcsI	WCGNNNNNNNCGW	1 cut(s)	640
PctI	GAATGC	1 cut(s)	2218
PdiI	GCCGGC	1 cut(s)	1807
PdmI	GAANNNNTTC	3 cut(s)	294, 1923, 1962
PfeI	GAWTC	6 cut(s)	113, 190, 560, 1133, 2272, 2402
PkrI	GCNGC	4 cut(s)	1021, 1024, 1692, 2134
PleI	GAGTC	4 cut(s)	137, 1645, 2072, 2244
PmaCI	CACGTG	1 cut(s)	1037
PmlI	CACGTG	1 cut(s)	1037
PpsI	GAGTC	4 cut(s)	137, 1645, 2072, 2244
Ppu21I	YACGTR	2 cut(s)	635, 1037
PscI	ACATGT	1 cut(s)	1172
PshBI	ATTAAT	1 cut(s)	2358
Psp1406I	AACGTT	1 cut(s)	1224
Psp6I	CCWGG	3 cut(s)	492, 1366, 1738
PspCI	CACGTG	1 cut(s)	1037
PspGI	CCWGG	3 cut(s)	492, 1366, 1738
PspN4I	GGNNCC	2 cut(s)	1633, 1804
PspPI	GGNCC	3 cut(s)	1364, 1658, 1736
PsuI	RGATCY	3 cut(s)	1275, 1440, 1972
RsaI	GTAC	6 cut(s)	125, 261, 304, 734, 865, 1770
RsaNI	GTAC	6 cut(s)	124, 260, 303, 733, 864, 1769
RseI	CAYNNNNRTG	1 cut(s)	1428
SacII	CCGCGG	1 cut(s)	2136
SapI	GCTCTTC	1 cut(s)	1116
SatI	GCNGC	4 cut(s)	1020, 1023, 1691, 2133
Sau3AI	GATC	8 cut(s)	775, 904, 937, 1066, 1275, 1440, 1848, 1972
Sau96I	GGNCC	3 cut(s)	1364, 1658, 1736
SchI	GAGTC	4 cut(s)	137, 1645, 2072, 2244
ScrFI	CCNGG	3 cut(s)	494, 1368, 1740
SduI	GDGCHC	4 cut(s)	492, 704, 754, 2305
SfaNI	GCATC	3 cut(s)	1078, 1238, 1525
SfcI	CTRYAG	3 cut(s)	870, 1146, 1279
Sfr303I	CCGCGG	1 cut(s)	2136
SfuI	TTCGAA	1 cut(s)	1910
SgrBI	CCGCGG	1 cut(s)	2136
SinI	GGWCC	2 cut(s)	1658, 1736
SmiMI	CAYNNNNRTG	1 cut(s)	1428
SmlI	CTYRAG	3 cut(s)	458, 2163, 2390
SmoI	CTYRAG	3 cut(s)	458, 2163, 2390
SphI	GCATGC	1 cut(s)	597
SsiI	CCGC	6 cut(s)	691, 1690, 1809, 1998, 2133, 2135
SspI	AATATT	2 cut(s)	316, 2026
SspMI	CTAG	6 cut(s)	197, 767, 876, 1332, 1655, 2060
StyD4I	CCNGG	3 cut(s)	492, 1366, 1738
StyI	CCWWGG	1 cut(s)	1635
TaaI	ACNGT	5 cut(s)	307, 717, 1571, 2050, 2180
TaqI	TCGA	5 cut(s)	966, 1300, 1543, 1910, 2081
TatI	WGTACW	4 cut(s)	123, 259, 302, 732
TauI	GCSGC	2 cut(s)	1693, 2135
TfiI	GAWTC	6 cut(s)	113, 190, 560, 1133, 2272, 2402
TscAI	CASTG	3 cut(s)	1090, 1576, 2185
TseFI	GTSAC	1 cut(s)	388
TseI	GCWGC	2 cut(s)	1019, 1022
Tsp45I	GTSAC	1 cut(s)	388
TspGWI	ACGGA	3 cut(s)	845, 1126, 2326
TspRI	CASTG	3 cut(s)	1090, 1576, 2185
VpaK11BI	GGWCC	2 cut(s)	1658, 1736
VspI	ATTAAT	1 cut(s)	2358
XapI	RAATTY	4 cut(s)	845, 888, 1324, 1667
XbaI	TCTAGA	1 cut(s)	766
XceI	RCATGY	4 cut(s)	225, 504, 597, 1176
XmnI	GAANNNNTTC	3 cut(s)	294, 1923, 1962
XspI	CTAG	6 cut(s)	197, 767, 876, 1332, 1655, 2060

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.