ion channel POLLUX-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1921 |
| AccB1I | GGYRCC | 1 cut(s) | 1901 |
| AccIII | TCCGGA | 1 cut(s) | 819 |
| AciI | CCGC | 5 cut(s) | 423, 790, 955, 1789, 2094 |
| AclI | AACGTT | 3 cut(s) | 277, 567, 1323 |
| AclWI | GGATC | 1 cut(s) | 2063 |
| AcoI | YGGCCR | 3 cut(s) | 787, 1044, 1790 |
| AcsI | RAATTY | 2 cut(s) | 987, 1766 |
| AcuI | CTGAAG | 4 cut(s) | 399, 740, 1466, 1725 |
| AcvI | CACGTG | 1 cut(s) | 1136 |
| AfaI | GTAC | 5 cut(s) | 221, 578, 964, 1426, 1869 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 164 |
| AflIII | ACRYGT | 3 cut(s) | 742, 1712, 1924 |
| AjiI | CACGTC | 2 cut(s) | 321, 1715 |
| AjnI | CCWGG | 3 cut(s) | 475, 591, 1837 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 378, 410, 732, 764, 2046, 2078 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1313, 1345 |
| Alw21I | GWGCWC | 2 cut(s) | 591, 2401 |
| Alw26I | GTCTC | 6 cut(s) | 55, 189, 610, 863, 1476, 2360 |
| AlwI | GGATC | 1 cut(s) | 2063 |
| Aor13HI | TCCGGA | 1 cut(s) | 819 |
| AoxI | GGCC | 4 cut(s) | 420, 787, 1044, 1790 |
| ApeKI | GCWGC | 4 cut(s) | 4, 31, 1118, 1121 |
| ApoI | RAATTY | 2 cut(s) | 987, 1766 |
| AseI | ATTAAT | 1 cut(s) | 2454 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 393, 2019, 2058 |
| AspLEI | GCGC | 1 cut(s) | 803 |
| AspS9I | GGNCC | 4 cut(s) | 420, 1463, 1757, 1835 |
| AsuHPI | GGTGA | 2 cut(s) | 284, 2330 |
| AsuII | TTCGAA | 2 cut(s) | 1613, 2006 |
| AvaII | GGWCC | 3 cut(s) | 1463, 1757, 1835 |
| AxyI | CCTNAGG | 1 cut(s) | 257 |
| BaeGI | GKGCMC | 1 cut(s) | 853 |
| BalI | TGGCCA | 1 cut(s) | 1046 |
| BanI | GGYRCC | 1 cut(s) | 1901 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 109, 141 |
| BbrPI | CACGTG | 1 cut(s) | 1136 |
| Bbv12I | GWGCWC | 2 cut(s) | 591, 2401 |
| BbvI | GCAGC | 4 cut(s) | 16, 43, 1105, 1108 |
| BccI | CCATC | 7 cut(s) | 7, 194, 304, 1222, 1779, 2218, 2380 |
| BceAI | ACGGC | 2 cut(s) | 802, 2252 |
| BciT130I | CCWGG | 3 cut(s) | 477, 593, 1839 |
| BciVI | GTATCC | 1 cut(s) | 128 |
| BcoDI | GTCTC | 6 cut(s) | 55, 189, 610, 863, 1476, 2360 |
| BfaI | CTAG | 6 cut(s) | 206, 920, 975, 1431, 1754, 2156 |
| BfmI | CTRYAG | 3 cut(s) | 765, 969, 1378 |
| BfuAI | ACCTGC | 1 cut(s) | 1921 |
| BfuI | GTATCC | 1 cut(s) | 128 |
| BglII | AGATCT | 2 cut(s) | 1374, 1539 |
| BisI | GCNGC | 7 cut(s) | 5, 32, 423, 790, 1119, 1122, 1790 |
| BlsI | GCNGC | 7 cut(s) | 6, 33, 424, 791, 1120, 1123, 1791 |
| BmcAI | AGTACT | 1 cut(s) | 1426 |
| Bme1390I | CCNGG | 3 cut(s) | 477, 593, 1839 |
| Bme18I | GGWCC | 3 cut(s) | 1463, 1757, 1835 |
| BmgBI | CACGTC | 2 cut(s) | 321, 1715 |
| BmgT120I | GGNCC | 4 cut(s) | 420, 1463, 1757, 1835 |
| BmiI | GGNNCC | 3 cut(s) | 421, 1732, 1903 |
| BmrFI | CCNGG | 3 cut(s) | 477, 593, 1839 |
| BmsI | GCATC | 4 cut(s) | 1011, 1177, 1337, 1624 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1468 |
| BpmI | CTGGAG | 1 cut(s) | 887 |
| Bpu10I | CCTNAGC | 2 cut(s) | 958, 1091 |
| Bpu14I | TTCGAA | 2 cut(s) | 1613, 2006 |
| BpuEI | CTTGAG | 4 cut(s) | 578, 1458, 2244, 2507 |
| Bsa29I | ATCGAT | 1 cut(s) | 1065 |
| BsaAI | YACGTR | 2 cut(s) | 576, 1136 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1638 |
| BsaI | GGTCTC | 1 cut(s) | 189 |
| BsaJI | CCNNGG | 3 cut(s) | 1734, 1838, 2268 |
| BsaWI | WCCGGW | 1 cut(s) | 819 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 164 |
| Bse1I | ACTGG | 4 cut(s) | 106, 348, 860, 2459 |
| Bse21I | CCTNAGG | 1 cut(s) | 257 |
| Bse3DI | GCAATG | 2 cut(s) | 810, 1569 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1638 |
| BseAI | TCCGGA | 1 cut(s) | 819 |
| BseBI | CCWGG | 3 cut(s) | 477, 593, 1839 |
| BseCI | ATCGAT | 1 cut(s) | 1065 |
| BseDI | CCNNGG | 3 cut(s) | 1734, 1838, 2268 |
| BseGI | GGATG | 6 cut(s) | 490, 1192, 1328, 1790, 2210, 2372 |
| BseJI | GATNNNNATC | 1 cut(s) | 1638 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 164 |
| BseMI | GCAATG | 2 cut(s) | 810, 1569 |
| BseMII | CTCAG | 6 cut(s) | 296, 853, 1082, 1308, 1458, 2508 |
| BseNI | ACTGG | 4 cut(s) | 106, 348, 860, 2459 |
| BseSI | GKGCMC | 1 cut(s) | 853 |
| BseX3I | CGGCCG | 1 cut(s) | 787 |
| BseXI | GCAGC | 4 cut(s) | 16, 43, 1105, 1108 |
| BsgI | GTGCAG | 2 cut(s) | 1949, 2168 |
| Bsh1285I | CGRYCG | 2 cut(s) | 790, 2541 |
| BshFI | GGCC | 4 cut(s) | 422, 789, 1046, 1792 |
| BshNI | GGYRCC | 1 cut(s) | 1901 |
| BshVI | ATCGAT | 1 cut(s) | 1065 |
| BsiEI | CGRYCG | 2 cut(s) | 790, 2541 |
| BsiHKAI | GWGCWC | 2 cut(s) | 591, 2401 |
| BsiSI | CCGG | 1 cut(s) | 820 |
| BslFI | GGGAC | 3 cut(s) | 1223, 1762, 2467 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 164 |
| BsmAI | GTCTC | 6 cut(s) | 55, 189, 610, 863, 1476, 2360 |
| BsmFI | GGGAC | 3 cut(s) | 1223, 1762, 2467 |
| BsmI | GAATGC | 1 cut(s) | 2314 |
| BsnI | GGCC | 4 cut(s) | 422, 789, 1046, 1792 |
| Bso31I | GGTCTC | 1 cut(s) | 189 |
| Bsp119I | TTCGAA | 2 cut(s) | 1613, 2006 |
| Bsp1286I | GDGCHC | 3 cut(s) | 591, 853, 2401 |
| Bsp13I | TCCGGA | 1 cut(s) | 819 |
| Bsp1407I | TGTACA | 1 cut(s) | 219 |
| Bsp143I | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| BspACI | CCGC | 5 cut(s) | 423, 790, 955, 1789, 2094 |
| BspANI | GGCC | 4 cut(s) | 422, 789, 1046, 1792 |
| BspCNI | CTCAG | 6 cut(s) | 295, 852, 1083, 1307, 1459, 2509 |
| BspDI | ATCGAT | 1 cut(s) | 1065 |
| BspEI | TCCGGA | 1 cut(s) | 819 |
| BspHI | TCATGA | 1 cut(s) | 940 |
| BspLI | GGNNCC | 3 cut(s) | 421, 1732, 1903 |
| BspMI | ACCTGC | 1 cut(s) | 1921 |
| BspPI | GGATC | 1 cut(s) | 2063 |
| BspQI | GCTCTTC | 1 cut(s) | 1215 |
| BspT104I | TTCGAA | 2 cut(s) | 1613, 2006 |
| BspT107I | GGYRCC | 1 cut(s) | 1901 |
| BspTNI | GGTCTC | 1 cut(s) | 189 |
| BsrDI | GCAATG | 2 cut(s) | 810, 1569 |
| BsrGI | TGTACA | 1 cut(s) | 219 |
| BsrI | ACTGG | 4 cut(s) | 106, 348, 860, 2459 |
| BssECI | CCNNGG | 3 cut(s) | 1734, 1838, 2268 |
| BssMI | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| BssT1I | CCWWGG | 1 cut(s) | 1734 |
| Bst2UI | CCWGG | 3 cut(s) | 477, 593, 1839 |
| Bst4CI | ACNGT | 5 cut(s) | 816, 902, 1670, 1872, 2276 |
| Bst6I | CTCTTC | 2 cut(s) | 1215, 1720 |
| BstAUI | TGTACA | 1 cut(s) | 219 |
| BstBAI | YACGTR | 2 cut(s) | 576, 1136 |
| BstBI | TTCGAA | 2 cut(s) | 1613, 2006 |
| BstC8I | GCNNGC | 3 cut(s) | 694, 1277, 2388 |
| BstDSI | CCRYGG | 1 cut(s) | 2268 |
| BstF5I | GGATG | 6 cut(s) | 490, 1192, 1328, 1790, 2210, 2372 |
| BstHHI | GCGC | 1 cut(s) | 803 |
| BstKTI | GATC | 7 cut(s) | 877, 1006, 1168, 1377, 1542, 2071, 2541 |
| BstMAI | GTCTC | 6 cut(s) | 55, 189, 610, 863, 1476, 2360 |
| BstMBI | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| BstMCI | CGRYCG | 2 cut(s) | 790, 2541 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 798, 1130, 1910, 2264, 2396 |
| BstNI | CCWGG | 3 cut(s) | 477, 593, 1839 |
| BstNSI | RCATGY | 4 cut(s) | 603, 1275, 1688, 1928 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1468 |
| BstSCI | CCNGG | 3 cut(s) | 475, 591, 1837 |
| BstSFI | CTRYAG | 3 cut(s) | 765, 969, 1378 |
| BstSLI | GKGCMC | 1 cut(s) | 853 |
| BstV1I | GCAGC | 4 cut(s) | 16, 43, 1105, 1108 |
| BstX2I | RGATCY | 3 cut(s) | 1374, 1539, 2068 |
| BstYI | RGATCY | 3 cut(s) | 1374, 1539, 2068 |
| BstZI | CGGCCG | 1 cut(s) | 787 |
| Bsu15I | ATCGAT | 1 cut(s) | 1065 |
| Bsu36I | CCTNAGG | 1 cut(s) | 257 |
| BsuI | GTATCC | 1 cut(s) | 128 |
| BsuRI | GGCC | 4 cut(s) | 422, 789, 1046, 1792 |
| BsuTUI | ATCGAT | 1 cut(s) | 1065 |
| BtgI | CCRYGG | 1 cut(s) | 2268 |
| BtgZI | GCGATG | 2 cut(s) | 213, 1647 |
| BtrI | CACGTC | 2 cut(s) | 321, 1715 |
| BtsCI | GGATG | 6 cut(s) | 490, 1192, 1328, 1790, 2210, 2372 |
| BtsIMutI | CAGTG | 3 cut(s) | 246, 1675, 2281 |
| BveI | ACCTGC | 1 cut(s) | 1921 |
| Cac8I | GCNNGC | 3 cut(s) | 694, 1277, 2388 |
| CciI | TCATGA | 1 cut(s) | 940 |
| CfoI | GCGC | 1 cut(s) | 803 |
| Cfr13I | GGNCC | 4 cut(s) | 420, 1463, 1757, 1835 |
| ClaI | ATCGAT | 1 cut(s) | 1065 |
| Csp6I | GTAC | 5 cut(s) | 220, 577, 963, 1425, 1868 |
| CviAII | CATG | 8 cut(s) | 137, 217, 469, 600, 941, 1272, 1685, 1925 |
| CviQI | GTAC | 5 cut(s) | 220, 577, 963, 1425, 1868 |
| DpnI | GATC | 7 cut(s) | 876, 1005, 1167, 1376, 1541, 2070, 2540 |
| DpnII | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| EaeI | YGGCCR | 3 cut(s) | 787, 1044, 1790 |
| EagI | CGGCCG | 1 cut(s) | 787 |
| Eam1104I | CTCTTC | 2 cut(s) | 1215, 1720 |
| EarI | CTCTTC | 2 cut(s) | 1215, 1720 |
| EciI | GGCGGA | 1 cut(s) | 2083 |
| EclXI | CGGCCG | 1 cut(s) | 787 |
| Eco130I | CCWWGG | 1 cut(s) | 1734 |
| Eco31I | GGTCTC | 1 cut(s) | 189 |
| Eco32I | GATATC | 1 cut(s) | 1069 |
| Eco47I | GGWCC | 3 cut(s) | 1463, 1757, 1835 |
| Eco52I | CGGCCG | 1 cut(s) | 787 |
| Eco57I | CTGAAG | 4 cut(s) | 399, 740, 1466, 1725 |
| Eco72I | CACGTG | 1 cut(s) | 1136 |
| Eco81I | CCTNAGG | 1 cut(s) | 257 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1463 |
| EcoRI | GAATTC | 1 cut(s) | 987 |
| EcoRII | CCWGG | 3 cut(s) | 475, 591, 1837 |
| EcoRV | GATATC | 1 cut(s) | 1069 |
| EcoT14I | CCWWGG | 1 cut(s) | 1734 |
| ErhI | CCWWGG | 1 cut(s) | 1734 |
| FaeI | CATG | 8 cut(s) | 140, 220, 472, 603, 944, 1275, 1688, 1928 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 378, 410, 1502, 1534 |
| FaqI | GGGAC | 3 cut(s) | 1223, 1762, 2467 |
| FatI | CATG | 8 cut(s) | 136, 216, 468, 599, 940, 1271, 1684, 1924 |
| FauNDI | CATATG | 1 cut(s) | 1108 |
| Fnu4HI | GCNGC | 7 cut(s) | 5, 32, 423, 790, 1119, 1122, 1790 |
| FokI | GGATG | 6 cut(s) | 497, 1199, 1315, 1797, 2197, 2359 |
| Fsp4HI | GCNGC | 7 cut(s) | 5, 32, 423, 790, 1119, 1122, 1790 |
| FspBI | CTAG | 6 cut(s) | 206, 920, 975, 1431, 1754, 2156 |
| GlaI | GCGC | 1 cut(s) | 802 |
| GluI | GCNGC | 7 cut(s) | 5, 32, 423, 790, 1119, 1122, 1790 |
| GsuI | CTGGAG | 1 cut(s) | 887 |
| HaeIII | GGCC | 4 cut(s) | 422, 789, 1046, 1792 |
| HapII | CCGG | 1 cut(s) | 820 |
| HhaI | GCGC | 1 cut(s) | 803 |
| Hin1II | CATG | 8 cut(s) | 140, 220, 472, 603, 944, 1275, 1688, 1928 |
| Hin6I | GCGC | 1 cut(s) | 801 |
| HinP1I | GCGC | 1 cut(s) | 801 |
| HincII | GTYRAC | 3 cut(s) | 1180, 1358, 2493 |
| HindII | GTYRAC | 3 cut(s) | 1180, 1358, 2493 |
| HindIII | AAGCTT | 1 cut(s) | 684 |
| HpaII | CCGG | 1 cut(s) | 820 |
| HphI | GGTGA | 2 cut(s) | 284, 2330 |
| Hpy166II | GTNNAC | 7 cut(s) | 898, 1180, 1358, 2173, 2272, 2279, 2493 |
| Hpy188III | TCNNGA | 4 cut(s) | 307, 820, 866, 941 |
| Hpy8I | GTNNAC | 7 cut(s) | 898, 1180, 1358, 2173, 2272, 2279, 2493 |
| HpyCH4III | ACNGT | 5 cut(s) | 816, 902, 1670, 1872, 2276 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 798, 1130, 1910, 2264, 2396 |
| Hsp92II | CATG | 8 cut(s) | 140, 220, 472, 603, 944, 1275, 1688, 1928 |
| HspAI | GCGC | 1 cut(s) | 801 |
| Kpn2I | TCCGGA | 1 cut(s) | 819 |
| Kzo9I | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| LguI | GCTCTTC | 1 cut(s) | 1215 |
| LmnI | GCTCC | 5 cut(s) | 519, 594, 822, 1736, 2383 |
| Lsp1109I | GCAGC | 4 cut(s) | 16, 43, 1105, 1108 |
| LweI | GCATC | 4 cut(s) | 1011, 1177, 1337, 1624 |
| MaeI | CTAG | 6 cut(s) | 206, 920, 975, 1431, 1754, 2156 |
| MaeIII | GTNAC | 3 cut(s) | 1085, 2158, 2251 |
| MalI | GATC | 7 cut(s) | 876, 1005, 1167, 1376, 1541, 2070, 2540 |
| MboI | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| MflI | RGATCY | 3 cut(s) | 1374, 1539, 2068 |
| MhlI | GDGCHC | 3 cut(s) | 591, 853, 2401 |
| MlsI | TGGCCA | 1 cut(s) | 1046 |
| MluNI | TGGCCA | 1 cut(s) | 1046 |
| MlyI | GAGTC | 8 cut(s) | 31, 57, 1359, 1478, 1744, 2168, 2340, 2362 |
| MmeI | TCCRAC | 2 cut(s) | 577, 2020 |
| Mox20I | TGGCCA | 1 cut(s) | 1046 |
| MroI | TCCGGA | 1 cut(s) | 819 |
| MroXI | GAANNNNTTC | 3 cut(s) | 393, 2019, 2058 |
| MscI | TGGCCA | 1 cut(s) | 1046 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1527 |
| Msp20I | TGGCCA | 1 cut(s) | 1046 |
| MspI | CCGG | 1 cut(s) | 820 |
| MspR9I | CCNGG | 3 cut(s) | 477, 593, 1839 |
| Mva1269I | GAATGC | 1 cut(s) | 2314 |
| MvaI | CCWGG | 3 cut(s) | 477, 593, 1839 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 798, 1130, 1910, 2264, 2396 |
| NdeI | CATATG | 1 cut(s) | 1108 |
| NdeII | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| NlaIII | CATG | 8 cut(s) | 140, 220, 472, 603, 944, 1275, 1688, 1928 |
| NlaIV | GGNNCC | 3 cut(s) | 421, 1732, 1903 |
| NspI | RCATGY | 4 cut(s) | 603, 1275, 1688, 1928 |
| NspV | TTCGAA | 2 cut(s) | 1613, 2006 |
| PagI | TCATGA | 1 cut(s) | 940 |
| PciI | ACATGT | 1 cut(s) | 1924 |
| PciSI | GCTCTTC | 1 cut(s) | 1215 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 739 |
| PctI | GAATGC | 1 cut(s) | 2314 |
| PdmI | GAANNNNTTC | 3 cut(s) | 393, 2019, 2058 |
| PfeI | GAWTC | 7 cut(s) | 209, 239, 289, 659, 1232, 2152, 2498 |
| PfoI | TCCNGGA | 1 cut(s) | 475 |
| PkrI | GCNGC | 7 cut(s) | 6, 33, 424, 791, 1120, 1123, 1791 |
| Ple19I | CGATCG | 1 cut(s) | 2541 |
| PleI | GAGTC | 8 cut(s) | 30, 57, 1359, 1477, 1744, 2168, 2340, 2362 |
| PmaCI | CACGTG | 1 cut(s) | 1136 |
| PmlI | CACGTG | 1 cut(s) | 1136 |
| PpsI | GAGTC | 8 cut(s) | 30, 57, 1359, 1477, 1744, 2168, 2340, 2362 |
| Ppu21I | YACGTR | 2 cut(s) | 576, 1136 |
| PpuMI | RGGWCCY | 1 cut(s) | 1463 |
| PscI | ACATGT | 1 cut(s) | 1924 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1468 |
| PshBI | ATTAAT | 1 cut(s) | 2454 |
| Psp1406I | AACGTT | 3 cut(s) | 277, 567, 1323 |
| Psp5II | RGGWCCY | 1 cut(s) | 1463 |
| Psp6I | CCWGG | 3 cut(s) | 475, 591, 1837 |
| PspCI | CACGTG | 1 cut(s) | 1136 |
| PspGI | CCWGG | 3 cut(s) | 475, 591, 1837 |
| PspN4I | GGNNCC | 3 cut(s) | 421, 1732, 1903 |
| PspPI | GGNCC | 4 cut(s) | 420, 1463, 1757, 1835 |
| PspPPI | RGGWCCY | 1 cut(s) | 1463 |
| PsuI | RGATCY | 3 cut(s) | 1374, 1539, 2068 |
| PvuI | CGATCG | 1 cut(s) | 2541 |
| RsaI | GTAC | 5 cut(s) | 221, 578, 964, 1426, 1869 |
| RsaNI | GTAC | 5 cut(s) | 220, 577, 963, 1425, 1868 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1527 |
| SapI | GCTCTTC | 1 cut(s) | 1215 |
| SatI | GCNGC | 7 cut(s) | 5, 32, 423, 790, 1119, 1122, 1790 |
| Sau3AI | GATC | 7 cut(s) | 874, 1003, 1165, 1374, 1539, 2068, 2538 |
| Sau96I | GGNCC | 4 cut(s) | 420, 1463, 1757, 1835 |
| ScaI | AGTACT | 1 cut(s) | 1426 |
| SchI | GAGTC | 8 cut(s) | 31, 57, 1359, 1478, 1744, 2168, 2340, 2362 |
| ScrFI | CCNGG | 3 cut(s) | 477, 593, 1839 |
| SduI | GDGCHC | 3 cut(s) | 591, 853, 2401 |
| SfaNI | GCATC | 4 cut(s) | 1011, 1177, 1337, 1624 |
| SfcI | CTRYAG | 3 cut(s) | 765, 969, 1378 |
| SfuI | TTCGAA | 2 cut(s) | 1613, 2006 |
| SinI | GGWCC | 3 cut(s) | 1463, 1757, 1835 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1527 |
| SmlI | CTYRAG | 4 cut(s) | 557, 1473, 2259, 2486 |
| SmoI | CTYRAG | 4 cut(s) | 557, 1473, 2259, 2486 |
| SsiI | CCGC | 5 cut(s) | 423, 790, 955, 1789, 2094 |
| SspI | AATATT | 1 cut(s) | 2122 |
| SspMI | CTAG | 6 cut(s) | 206, 920, 975, 1431, 1754, 2156 |
| StyD4I | CCNGG | 3 cut(s) | 475, 591, 1837 |
| StyI | CCWWGG | 1 cut(s) | 1734 |
| TaaI | ACNGT | 5 cut(s) | 816, 902, 1670, 1872, 2276 |
| TaqI | TCGA | 7 cut(s) | 797, 1065, 1399, 1613, 1642, 2006, 2177 |
| TatI | WGTACW | 2 cut(s) | 219, 1424 |
| TauI | GCSGC | 3 cut(s) | 425, 792, 1792 |
| TfiI | GAWTC | 7 cut(s) | 209, 239, 289, 659, 1232, 2152, 2498 |
| TscAI | CASTG | 3 cut(s) | 253, 1675, 2281 |
| TseI | GCWGC | 4 cut(s) | 4, 31, 1118, 1121 |
| TspGWI | ACGGA | 6 cut(s) | 748, 1189, 1199, 1225, 2396, 2422 |
| TspRI | CASTG | 3 cut(s) | 253, 1675, 2281 |
| VpaK11BI | GGWCC | 3 cut(s) | 1463, 1757, 1835 |
| VspI | ATTAAT | 1 cut(s) | 2454 |
| XapI | RAATTY | 2 cut(s) | 987, 1766 |
| XceI | RCATGY | 4 cut(s) | 603, 1275, 1688, 1928 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1645 |
| XmnI | GAANNNNTTC | 3 cut(s) | 393, 2019, 2058 |
| XspI | CTAG | 6 cut(s) | 206, 920, 975, 1431, 1754, 2156 |
| ZrmI | AGTACT | 1 cut(s) | 1426 |