Rh4DG022100

ion channel POLLUX-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001261
Physical Location & Seq
Forward (+)
3628866 .. 3638545
9680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG022100.1

Sequence Viewer

Length: 1596 bp
ATGGTTGTGAGTCAGTTGCAGCTATGCGTTTTGCCACATCGGTTTCCGAGACTCAACCTCTCTCCTTCTTCTTCTCCAAACAGGAAATATATGCCGTGCCAGTTTTGGTGTATGAAATCTTCATCTTTACATGGACGTAATTTCATAACTCCAAACAGAGGAAAAGGCAAGGTCAGGTCTCAAAAGACAGGCAATAAACTAGATTCCACTATGTACATAAATGTTGAAGATGACTCGGACACTGAACTTCTTAGGATAGAACAAGTAAAAAGTTCTCAGATTCACCTAGCTAAAGTCTTGATGGGATTGACGTGCTTATATATTCCACTTAGACTGGCAAAGCTAAATGTCGTTGATACTTTCATTAAGATTGTCCAAGAAAGACTTCTCTGCACTGTTCAAAATTTAGGGGCCGCCACCTTGCCTTTTGCCTGTGCTTCTAATTCTCTGAATAAACCACTGCCTCTTGGATTGGATGTGTCGTTGCCATCATTTCAAGATGTTAGATGGAGCTTTCAACGATTATTGTATCTTTTTAACATCCAACTTGAGAAAAATGTTGCTACGTTCTTCATAGTGCTCCTGGTAGCATGTTTCTCATTTGTTATCATTGGTGGTTTCCTATTCTTCAAGTTTAGGGGTAGTAAAGAATCTTTGGAGGACTGCTTCTGGGAAGCTTGGGCATGCCTATGTTCATCATCGACACATCTAAAGCAAAGAACACGTATTGAACGTGTTATCGGTTTCGTTCTTGCTATATGGGGCATATTATTTTACTCCCGCTTATTGAGCACAATGACTGAACAGTTCCGGAGTAATATGTACAAGCTCAGAGAAGGGGCACAAATGCAAGTACTGGAGACTGATCACATCATTATTTGTGGAGTGAACAGTCATCTTTCTTTCATACTAAAGCAAATAAACAAGTATCATGAATTTGCTGTCCGCTTAGGTACTGCTACAGCTAGGAAGCAAAGAATTCTTCTTATGTCTGACTTACCAAGAAAGCAGATGGACAAGTTAGCTGACCATATTGCCAAAGATTTTAACCATATCGATATCCTTACAAAAAGGGTCGAAATAGCTGACCTTGCATTAAAGAACCTCTCTTCAGATCCAAACAGGGCAGACAAGCAATCCGCCTATTCCCTTCTTCTTGCTGAAAATATTTGCAACAAACTTGGCGTAAAGGTGCAGAATCTAGTTGCGGAAATCGTGGACTCGAAATTGGGGAAACAAATCACAAGAATAAAGCCATCCCTGACTTATATTGCCGCGGAGGAAGTAATGAGCCTCGTAACAGCTCAAGTTGCTGAGGACAGTGAACTAAATGAGGTGTGGAAAGACATTCTGGATGCAGACGGTGATGAAATATATGTGAAGGATATCAGACTCTATATGAAAGAAGGAGAGACTCCATCCTTCACGGAGCTTGCTGAAAGAGCTCATTTACGGAAGGAAGTTGCCATAGGTTATGTGAAAAACAATAAGAAGGTTATTAATCCAGTTCCCAAGTCAGAACCCCTCTCACTTGAGTTGACAGATTCGTTAATAGTTATATCTGAACTTGAAGGAGAACAACCGATCGTTATGTAG

Protein Analysis

531

Amino Acids

60.3

Weight (kDa)

8.83

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Castor_Poll_mid PF06241 427 - 525 9.3e-06 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1277
AccIII TCCGGA 1 cut(s) 810
AciI CCGC 7 cut(s) 414, 781, 946, 1140, 1208, 1275, 1277
AclWI GGATC 1 cut(s) 1109
AcsI RAATTY 3 cut(s) 403, 935, 978
AcuI CTGAAG 1 cut(s) 1095
AfaI GTAC 4 cut(s) 215, 824, 855, 955
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 2 cut(s) 722, 733
AgsI TTSAA 7 cut(s) 227, 401, 497, 518, 631, 731, 1571
AjiI CACGTC 1 cut(s) 312
AjnI CCWGG 1 cut(s) 582
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
Alw21I GWGCWC 3 cut(s) 582, 794, 1447
Alw26I GTCTC 4 cut(s) 43, 183, 854, 1406
AlwI GGATC 1 cut(s) 1109
Aor13HI TCCGGA 1 cut(s) 810
AoxI GGCC 1 cut(s) 411
ApeKI GCWGC 1 cut(s) 19
ApoI RAATTY 3 cut(s) 403, 935, 978
AseI ATTAAT 1 cut(s) 1500
Asp700I GAANNNNTTC 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 275, 1376
BaeGI GKGCMC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 1447
BarI GAAGNNNNNNTAC 2 cut(s) 103, 135
Bbv12I GWGCWC 3 cut(s) 582, 794, 1447
BbvCI CCTCAGC 1 cut(s) 1314
BbvI GCAGC 1 cut(s) 31
BccI CCATC 6 cut(s) 295, 496, 501, 1006, 1264, 1426
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 1 cut(s) 584
BclI TGATCA 1 cut(s) 865
BcoDI GTCTC 4 cut(s) 43, 183, 854, 1406
BfaI CTAG 4 cut(s) 200, 287, 966, 1202
BfmI CTRYAG 1 cut(s) 960
BisI GCNGC 3 cut(s) 20, 414, 1275
BlsI GCNGC 3 cut(s) 21, 415, 1276
BmcAI AGTACT 1 cut(s) 855
Bme1390I CCNGG 1 cut(s) 584
BmgBI CACGTC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 1 cut(s) 412
BmrFI CCNGG 1 cut(s) 584
BmsI GCATC 1 cut(s) 1345
BpmI CTGGAG 1 cut(s) 878
Bpu10I CCTNAGC 2 cut(s) 949, 1314
BpuEI CTTGAG 3 cut(s) 569, 1290, 1553
Bsa29I ATCGAT 1 cut(s) 1056
BsaAI YACGTR 1 cut(s) 725
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 1275
BsaWI WCCGGW 1 cut(s) 810
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse1I ACTGG 4 cut(s) 100, 339, 861, 1505
BseAI TCCGGA 1 cut(s) 810
BseBI CCWGG 1 cut(s) 584
BseCI ATCGAT 1 cut(s) 1056
BseDI CCNNGG 1 cut(s) 1275
BseGI GGATG 5 cut(s) 481, 540, 1256, 1360, 1418
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 3 cut(s) 290, 844, 1305
BseNI ACTGG 4 cut(s) 100, 339, 861, 1505
BseSI GKGCMC 1 cut(s) 844
BseXI GCAGC 1 cut(s) 31
BsgI GTGCAG 2 cut(s) 376, 1214
Bsh1236I CGCG 1 cut(s) 1277
Bsh1285I CGRYCG 1 cut(s) 1587
BshFI GGCC 1 cut(s) 413
BshVI ATCGAT 1 cut(s) 1056
BsiEI CGRYCG 1 cut(s) 1587
BsiHKAI GWGCWC 3 cut(s) 582, 794, 1447
BsiSI CCGG 1 cut(s) 811
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 4 cut(s) 43, 183, 854, 1406
BsnI GGCC 1 cut(s) 413
Bso31I GGTCTC 1 cut(s) 183
Bsp1286I GDGCHC 4 cut(s) 582, 794, 844, 1447
Bsp13I TCCGGA 1 cut(s) 810
Bsp1407I TGTACA 2 cut(s) 213, 822
Bsp143I GATC 3 cut(s) 865, 1114, 1584
BspACI CCGC 7 cut(s) 414, 781, 946, 1140, 1208, 1275, 1277
BspANI GGCC 1 cut(s) 413
BspCNI CTCAG 3 cut(s) 289, 843, 1306
BspDI ATCGAT 1 cut(s) 1056
BspEI TCCGGA 1 cut(s) 810
BspFNI CGCG 1 cut(s) 1277
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 1 cut(s) 412
BspPI GGATC 1 cut(s) 1109
BspTNI GGTCTC 1 cut(s) 183
BsrGI TGTACA 2 cut(s) 213, 822
BsrI ACTGG 4 cut(s) 100, 339, 861, 1505
BssECI CCNNGG 1 cut(s) 1275
BssMI GATC 3 cut(s) 865, 1114, 1584
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 5 cut(s) 397, 807, 893, 1322, 1364
Bst6I CTCTTC 1 cut(s) 1114
BstAUI TGTACA 2 cut(s) 213, 822
BstBAI YACGTR 1 cut(s) 725
BstC8I GCNNGC 2 cut(s) 685, 1434
BstDEI CTNAG 6 cut(s) 251, 276, 329, 830, 949, 1314
BstDSI CCRYGG 1 cut(s) 1275
BstF5I GGATG 5 cut(s) 481, 540, 1256, 1360, 1418
BstFNI CGCG 1 cut(s) 1277
BstKTI GATC 3 cut(s) 868, 1117, 1587
BstMAI GTCTC 4 cut(s) 43, 183, 854, 1406
BstMBI GATC 3 cut(s) 865, 1114, 1584
BstMCI CGRYCG 1 cut(s) 1587
BstMWI GCNNNNNNNGC 4 cut(s) 789, 1091, 1310, 1442
BstNI CCWGG 1 cut(s) 584
BstNSI RCATGY 2 cut(s) 594, 687
BstSCI CCNGG 1 cut(s) 582
BstSFI CTRYAG 1 cut(s) 960
BstSLI GKGCMC 1 cut(s) 844
BstUI CGCG 1 cut(s) 1277
BstV1I GCAGC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 1114
BstYI RGATCY 1 cut(s) 1114
Bsu15I ATCGAT 1 cut(s) 1056
BsuRI GGCC 1 cut(s) 413
BsuTUI ATCGAT 1 cut(s) 1056
BtgI CCRYGG 1 cut(s) 1275
BtrI CACGTC 1 cut(s) 312
BtsCI GGATG 5 cut(s) 481, 540, 1256, 1360, 1418
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 4 cut(s) 240, 393, 458, 1327
Cac8I GCNNGC 2 cut(s) 685, 1434
CciI TCATGA 1 cut(s) 931
Cfr13I GGNCC 1 cut(s) 411
Cfr42I CCGCGG 1 cut(s) 1278
ClaI ATCGAT 1 cut(s) 1056
Csp6I GTAC 4 cut(s) 214, 823, 854, 954
CviAII CATG 4 cut(s) 131, 591, 684, 932
CviQI GTAC 4 cut(s) 214, 823, 854, 954
DdeI CTNAG 6 cut(s) 251, 276, 329, 830, 949, 1314
DpnI GATC 3 cut(s) 867, 1116, 1586
DpnII GATC 3 cut(s) 865, 1114, 1584
Eam1104I CTCTTC 1 cut(s) 1114
EarI CTCTTC 1 cut(s) 1114
EciI GGCGGA 1 cut(s) 1129
Ecl136II GAGCTC 1 cut(s) 1445
Eco24I GRGCYC 1 cut(s) 1447
Eco31I GGTCTC 1 cut(s) 183
Eco32I GATATC 2 cut(s) 1060, 1387
Eco53kI GAGCTC 1 cut(s) 1445
Eco57I CTGAAG 1 cut(s) 1095
EcoICRI GAGCTC 1 cut(s) 1445
EcoRI GAATTC 1 cut(s) 978
EcoRII CCWGG 1 cut(s) 582
EcoRV GATATC 2 cut(s) 1060, 1387
EcoT38I GRGCYC 1 cut(s) 1447
FaeI CATG 4 cut(s) 134, 594, 687, 935
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FatI CATG 4 cut(s) 130, 590, 683, 931
FauI CCCGC 1 cut(s) 788
FbaI TGATCA 1 cut(s) 865
Fnu4HI GCNGC 3 cut(s) 20, 414, 1275
FokI GGATG 5 cut(s) 488, 527, 1243, 1367, 1405
FriOI GRGCYC 1 cut(s) 1447
Fsp4HI GCNGC 3 cut(s) 20, 414, 1275
FspBI CTAG 4 cut(s) 200, 287, 966, 1202
GluI GCNGC 3 cut(s) 20, 414, 1275
GsuI CTGGAG 1 cut(s) 878
HaeIII GGCC 1 cut(s) 413
HapII CCGG 1 cut(s) 811
Hin1II CATG 4 cut(s) 134, 594, 687, 935
HincII GTYRAC 1 cut(s) 1539
HindII GTYRAC 1 cut(s) 1539
HindIII AAGCTT 1 cut(s) 675
HpaII CCGG 1 cut(s) 811
HphI GGTGA 2 cut(s) 275, 1376
Hpy166II GTNNAC 4 cut(s) 889, 1219, 1325, 1539
Hpy188III TCNNGA 5 cut(s) 298, 497, 811, 932, 1352
Hpy8I GTNNAC 4 cut(s) 889, 1219, 1325, 1539
HpyAV CCTTC 9 cut(s) 75, 830, 1160, 1375, 1400, 1432, 1450, 1486, 1565
HpyCH4III ACNGT 5 cut(s) 397, 807, 893, 1322, 1364
HpyCH4IV ACGT 5 cut(s) 136, 311, 566, 724, 733
HpyCH4V TGCA 7 cut(s) 19, 393, 850, 1094, 1173, 1195, 1358
HpyF10VI GCNNNNNNNGC 4 cut(s) 789, 1091, 1310, 1442
HpyF3I CTNAG 6 cut(s) 251, 276, 329, 830, 949, 1314
HpySE526I ACGT 5 cut(s) 136, 311, 566, 724, 733
Hsp92II CATG 4 cut(s) 134, 594, 687, 935
Kpn2I TCCGGA 1 cut(s) 810
Ksp22I TGATCA 1 cut(s) 865
KspI CCGCGG 1 cut(s) 1278
Kzo9I GATC 3 cut(s) 865, 1114, 1584
LmnI GCTCC 3 cut(s) 510, 585, 1429
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 1345
MaeI CTAG 4 cut(s) 200, 287, 966, 1202
MaeII ACGT 5 cut(s) 136, 311, 566, 724, 733
MaeIII GTNAC 1 cut(s) 1297
MalI GATC 3 cut(s) 867, 1116, 1586
MboI GATC 3 cut(s) 865, 1114, 1584
MboII GAAGA 9 cut(s) 60, 63, 111, 239, 562, 619, 974, 1101, 1145
MflI RGATCY 1 cut(s) 1114
MhlI GDGCHC 4 cut(s) 582, 794, 844, 1447
MluCI AATT 6 cut(s) 139, 403, 442, 935, 978, 1226
MlyI GAGTC 6 cut(s) 19, 45, 227, 1214, 1386, 1408
MmeI TCCRAC 1 cut(s) 568
MroI TCCGGA 1 cut(s) 810
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 6 cut(s) 366, 537, 1047, 1097, 1500, 1550
MslI CAYNNNNRTG 1 cut(s) 688
MspA1I CMGCKG 1 cut(s) 1277
MspI CCGG 1 cut(s) 811
MspR9I CCNGG 1 cut(s) 584
MvaI CCWGG 1 cut(s) 584
MvnI CGCG 1 cut(s) 1277
MwoI GCNNNNNNNGC 4 cut(s) 789, 1091, 1310, 1442
NdeII GATC 3 cut(s) 865, 1114, 1584
NlaIII CATG 4 cut(s) 134, 594, 687, 935
NlaIV GGNNCC 1 cut(s) 412
NspI RCATGY 2 cut(s) 594, 687
PaeI GCATGC 1 cut(s) 687
PagI TCATGA 1 cut(s) 931
PcsI WCGNNNNNNNCGW 1 cut(s) 730
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 5 cut(s) 203, 280, 650, 1198, 1544
PkrI GCNGC 3 cut(s) 21, 415, 1276
Ple19I CGATCG 1 cut(s) 1587
PleI GAGTC 6 cut(s) 18, 45, 227, 1214, 1386, 1408
PpsI GAGTC 6 cut(s) 18, 45, 227, 1214, 1386, 1408
Ppu21I YACGTR 1 cut(s) 725
PshBI ATTAAT 1 cut(s) 1500
Psp124BI GAGCTC 1 cut(s) 1447
Psp6I CCWGG 1 cut(s) 582
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 1 cut(s) 412
PspPI GGNCC 1 cut(s) 411
PsuI RGATCY 1 cut(s) 1114
PvuI CGATCG 1 cut(s) 1587
RsaI GTAC 4 cut(s) 215, 824, 855, 955
RsaNI GTAC 4 cut(s) 214, 823, 854, 954
RseI CAYNNNNRTG 1 cut(s) 688
SacI GAGCTC 1 cut(s) 1447
SacII CCGCGG 1 cut(s) 1278
SaqAI TTAA 6 cut(s) 366, 537, 1047, 1097, 1500, 1550
SatI GCNGC 3 cut(s) 20, 414, 1275
Sau3AI GATC 3 cut(s) 865, 1114, 1584
Sau96I GGNCC 1 cut(s) 411
ScaI AGTACT 1 cut(s) 855
SchI GAGTC 6 cut(s) 19, 45, 227, 1214, 1386, 1408
ScrFI CCNGG 1 cut(s) 584
SduI GDGCHC 4 cut(s) 582, 794, 844, 1447
SfaNI GCATC 1 cut(s) 1345
SfcI CTRYAG 1 cut(s) 960
Sfr303I CCGCGG 1 cut(s) 1278
SgrBI CCGCGG 1 cut(s) 1278
SmiMI CAYNNNNRTG 1 cut(s) 688
SmlI CTYRAG 3 cut(s) 548, 1305, 1532
SmoI CTYRAG 3 cut(s) 548, 1305, 1532
SphI GCATGC 1 cut(s) 687
Sse9I AATT 6 cut(s) 139, 403, 442, 935, 978, 1226
SsiI CCGC 7 cut(s) 414, 781, 946, 1140, 1208, 1275, 1277
SspI AATATT 1 cut(s) 1168
SspMI CTAG 4 cut(s) 200, 287, 966, 1202
SstI GAGCTC 1 cut(s) 1447
StyD4I CCNGG 1 cut(s) 582
TaaI ACNGT 5 cut(s) 397, 807, 893, 1322, 1364
TaiI ACGT 5 cut(s) 139, 314, 569, 727, 736
TaqI TCGA 4 cut(s) 701, 1056, 1077, 1223
TasI AATT 6 cut(s) 139, 403, 442, 935, 978, 1226
TatI WGTACW 3 cut(s) 213, 822, 853
TauI GCSGC 2 cut(s) 416, 1277
TfiI GAWTC 5 cut(s) 203, 280, 650, 1198, 1544
Tru1I TTAA 6 cut(s) 366, 537, 1047, 1097, 1500, 1550
Tru9I TTAA 6 cut(s) 366, 537, 1047, 1097, 1500, 1550
TscAI CASTG 4 cut(s) 247, 400, 465, 1327
TseI GCWGC 1 cut(s) 19
TspGWI ACGGA 2 cut(s) 1442, 1468
TspRI CASTG 4 cut(s) 247, 400, 465, 1327
VspI ATTAAT 1 cut(s) 1500
XapI RAATTY 3 cut(s) 403, 935, 978
XceI RCATGY 2 cut(s) 594, 687
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 4 cut(s) 200, 287, 966, 1202
ZrmI AGTACT 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.