Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001261

Physical Location & Seq

Genomic Coordinates Forward (+)

5955746 .. 5961059

Gene Structure 5314 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1069300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1521 bp

ATGACTTTTCGGGGAGGGACCAGGTTGTCCACGTTGGATTTCACGAAAAATCCCAAATTTTCAAGATTTCCCCACTTTTCTGTTTCTTTTATTTTCCCAAAATTCCACTTGTTTTGTTTGAAGTTTCTGTTGTTTCTCTCATCGGCCCCAGTTTCCTTGTTTCTTTCCTCTGAAACCTCGAAACTTCTCTGGCTGACTGTAACAAACATGGTTCTTTTGAGTAGTCAGCTGCACAGATTGCCGCTCTGCGTTTTGCCTCCTCGGGTTCCGATCTCCCACCGTTTTTCTTCCCCAAAAAGGAAATTAATGCCATGCCAATTTTGGTGGATCAAATCTTCGTCTTTACATGCAAGTAGTTTCATAGTACACAACAGAGCGAAATGTGAGGCCAGTTCTCAAAGGGCAGAAAATAAGCTAGATTCCACTGTGTATATAAATGTTGCAGATGATTTGAATGACCAACTCTTTTATACAGACCAAATTAATATTTCTCAGATCATCCAAGCAAAACTTCCCTCTATCATTCAAACTTTCGGGGCTGCCACCTTGCCCTTTGCCTGTGTCTCTAATTCATTGAATAAACCTGTGCCTCTTCGGTTGGATGTGTCTTTTCCTTCATTTCAAGACATTAGATGGAGCTTTGCACAGTTACTGTATCTATTTAACATCCAACTTGAAAAGAACGTTGCCACGTTCTTTCTGGTGCTCCTGGTAGCATGCTTCTCATTTGTTGTCATTGGTGGTTTCCTATTCTACAAGTATAGGGGTAGTAATGAGTCTTTGGATGACTGCTTCTGGGAGGCTTGGGCATGTCTGTGTTCATCATCGACACATTTAAAACAAAGAACACGAATTGAACGTGTTATTGGTTTTGTTCTTTCAATATGGGGCATATTATTTTACTCTCGCTTGTTGAGCACAATGACCGAACAGTTTAGGACTAAAAGAAGGCGCACAATGCAAGTTCTGGAGACTGATCATATCATTATATGTGGAGTGAACAGTCATCTTTCCTTCATGCTAAAGCAACTAAACAAGTATCACGAACTTGCTGTCTGCTTAGGTACTGCTACAGCTAGAATTCTTCTTATGTCCGATCTCCCAAGGAAGCAGATGGACAAACTTGCGGACAATTTAGACAAAGATCTTATAGATATTGATATCCTTACAAAGAGGTATGAAGTTGACACTGATGCTTTTTTGTCTGTTCTGGCTCTTCAACCTATTCCCAACATGGAATCTGTCCCCACAGTTGTTGAGGTTTCAAGTTCCAGTACATGTGAACTCTTAAAGTCGATCTCAGGGTTAAAAGTAGAGCCTGTGGAAAATGGGGCGTCAAAATTATTTGTACAATGTCCCCACAAAAATGTATTTAATCTTTGCAGTTTCCCAAGTCTAGCAGGGCTCAAGTACAGGAGATTACAACATGGGTTTCAAGAGGCAGTTGTTTGTGGGCTTTATAGGAACGGGAAGATAGACTTCCACCCCAATGACAGTGAAATCTTGCGGGAAACTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Castor_Poll_mid

Protein Analysis

507

Amino Acids

57.71

Weight (kDa)

9.06

Isoelectric Point (pI)

46.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Castor_Poll_mid	PF06241	454 - 506	6.3e-15	Castor and Pollux, part of voltage-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001261)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02940 AT5G02940 AT5G43745
fragaria_vesca	FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230
malus_domestica	MD11G1069300.v1.1 MD13G1204000.v1.1 MD16G1206000.v1.1
prunus_persica	Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1
pyrus_communis	pycom11g05780 pycom13g17720
rosa_chinensis	RchiOBHm_Chr4g0389551 RchiOBHm_Chr4g0389561 RchiOBHm_Chr4g0389571 RchiOBHm_Chr4g0389581
rosa_laevigata	RLG00000009970 RLG00000009971 RLG00000009972
rosa_multiflora	Rmu_co8238337.1_g000001 Rmu_co8398985.1_g000001 Rmu_sc0010540.1_g000001 Rmu_ssc0000478.1_g000001 Rmu_ssc0000478.1_g000002 Rmu_ssc0000478.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335510 Rroxscaffold_5G00335520 Rroxscaffold_5G00335530
rosa_rugosa	Rorug03G0324000
rosa_samantha	Rh2BG306500 Rh2CG284600 Rh4AG028300 Rh4AG028400 Rh4AG028500 Rh4BG021500 Rh4BG021600 Rh4BG021700 Rh4CG030500 Rh4CG030700 Rh4DG022100 Rh4DG022200 Rh4DG022300
rosa_wichuraiana	Rw4G002110 Rw4G002120 Rw4G002130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	25
AccBSI	CCGCTC	1 cut(s)	244
AciI	CCGC	3 cut(s)	242, 1127, 1507
AclI	AACGTT	1 cut(s)	684
AclWI	GGATC	1 cut(s)	335
AcsI	RAATTY	3 cut(s)	56, 101, 1080
AcyI	GRCGYC	1 cut(s)	1334
AfaI	GTAC	5 cut(s)	366, 1066, 1276, 1350, 1412
AfiI	CCNNNNNNNGG	1 cut(s)	297
AflIII	ACRYGT	2 cut(s)	859, 1277
AjnI	CCWGG	2 cut(s)	20, 708
AjuI	GAANNNNNNNTTGG	4 cut(s)	495, 527, 849, 881
AluBI	AGCT	4 cut(s)	229, 415, 639, 1076
AluI	AGCT	4 cut(s)	229, 415, 639, 1076
Alw21I	GWGCWC	2 cut(s)	708, 920
Alw26I	GTCTC	2 cut(s)	568, 965
AlwI	GGATC	1 cut(s)	335
AlwNI	CAGNNNCTG	1 cut(s)	652
Ama87I	CYCGRG	1 cut(s)	261
AoxI	GGCC	2 cut(s)	144, 387
ApeKI	GCWGC	2 cut(s)	229, 539
ApoI	RAATTY	3 cut(s)	56, 101, 1080
ArsI	GACNNNNNNTTYG	2 cut(s)	1038, 1070
AseI	ATTAAT	2 cut(s)	305, 483
AspLEI	GCGC	1 cut(s)	954
AspS9I	GGNCC	2 cut(s)	18, 145
AvaI	CYCGRG	1 cut(s)	261
AvaII	GGWCC	1 cut(s)	18
BanII	GRGCYC	1 cut(s)	1407
Bbv12I	GWGCWC	2 cut(s)	708, 920
BbvI	GCAGC	2 cut(s)	216, 526
BccI	CCATC	2 cut(s)	627, 1108
BciT130I	CCWGG	2 cut(s)	22, 710
BclI	TGATCA	1 cut(s)	976
BcoDI	GTCTC	2 cut(s)	568, 965
BfaI	CTAG	3 cut(s)	416, 1077, 1397
BfmI	CTRYAG	1 cut(s)	1071
BglII	AGATCT	1 cut(s)	1144
BisI	GCNGC	3 cut(s)	230, 242, 540
BlsI	GCNGC	3 cut(s)	231, 243, 541
Bme1390I	CCNGG	2 cut(s)	22, 710
Bme18I	GGWCC	1 cut(s)	18
BmeT110I	CYCGRG	1 cut(s)	261
BmgT120I	GGNCC	2 cut(s)	18, 145
BmiI	GGNNCC	3 cut(s)	19, 147, 267
BmrFI	CCNGG	2 cut(s)	22, 710
BmrI	ACTGGG	1 cut(s)	143
BmsI	GCATC	1 cut(s)	1183
BmuI	ACTGGG	1 cut(s)	143
BpmI	CTGGAG	1 cut(s)	989
Bpu10I	CCTNAGC	1 cut(s)	1060
BpuEI	CTTGAG	1 cut(s)	1391
BsaHI	GRCGYC	1 cut(s)	1334
BsaJI	CCNNGG	2 cut(s)	260, 1103
Bsc4I	CCNNNNNNNGG	1 cut(s)	297
Bse1I	ACTGG	3 cut(s)	149, 390, 1272
BseBI	CCWGG	2 cut(s)	22, 710
BseDI	CCNNGG	2 cut(s)	260, 1103
BseGI	GGATG	4 cut(s)	498, 607, 666, 790
BseLI	CCNNNNNNNGG	1 cut(s)	297
BseMII	CTCAG	2 cut(s)	506, 1314
BseNI	ACTGG	3 cut(s)	149, 390, 1272
BseRI	GAGGAG	1 cut(s)	249
BseXI	GCAGC	2 cut(s)	216, 526
BsgI	GTGCAG	1 cut(s)	215
BshFI	GGCC	2 cut(s)	146, 389
BsiHKAI	GWGCWC	2 cut(s)	708, 920
BsiHKCI	CYCGRG	1 cut(s)	261
BslFI	GGGAC	3 cut(s)	31, 1229, 1341
BslI	CCNNNNNNNGG	1 cut(s)	297
BsmAI	GTCTC	2 cut(s)	568, 965
BsmFI	GGGAC	3 cut(s)	31, 1229, 1341
BsnI	GGCC	2 cut(s)	146, 389
BsoBI	CYCGRG	1 cut(s)	261
Bsp1286I	GDGCHC	3 cut(s)	708, 920, 1407
Bsp1407I	TGTACA	1 cut(s)	1348
Bsp143I	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
BspACI	CCGC	3 cut(s)	242, 1127, 1507
BspANI	GGCC	2 cut(s)	146, 389
BspCNI	CTCAG	2 cut(s)	505, 1313
BspLI	GGNNCC	3 cut(s)	19, 147, 267
BspPI	GGATC	1 cut(s)	335
BspQI	GCTCTTC	1 cut(s)	1221
BsrBI	CCGCTC	1 cut(s)	244
BsrGI	TGTACA	1 cut(s)	1348
BsrI	ACTGG	3 cut(s)	149, 390, 1272
BssECI	CCNNGG	2 cut(s)	260, 1103
BssMI	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
BssNI	GRCGYC	1 cut(s)	1334
BssT1I	CCWWGG	1 cut(s)	1103
Bst2UI	CCWGG	2 cut(s)	22, 710
Bst4CI	ACNGT	9 cut(s)	199, 281, 427, 648, 654, 933, 1004, 1252, 1496
Bst6I	CTCTTC	2 cut(s)	597, 1221
BstACI	GRCGYC	1 cut(s)	1334
BstAPI	GCANNNNNTGC	1 cut(s)	238
BstAUI	TGTACA	1 cut(s)	1348
BstC8I	GCNNGC	1 cut(s)	718
BstDEI	CTNAG	3 cut(s)	492, 1060, 1300
BstF5I	GGATG	4 cut(s)	498, 607, 666, 790
BstHHI	GCGC	1 cut(s)	954
BstKTI	GATC	7 cut(s)	273, 330, 498, 979, 1099, 1147, 1299
BstMAI	GTCTC	2 cut(s)	568, 965
BstMBI	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
BstMWI	GCNNNNNNNGC	3 cut(s)	238, 915, 958
BstNI	CCWGG	2 cut(s)	22, 710
BstNSI	RCATGY	4 cut(s)	350, 720, 813, 1281
BstSCI	CCNGG	2 cut(s)	20, 708
BstSFI	CTRYAG	1 cut(s)	1071
BstV1I	GCAGC	2 cut(s)	216, 526
BstX2I	RGATCY	1 cut(s)	1144
BstYI	RGATCY	1 cut(s)	1144
BsuRI	GGCC	2 cut(s)	146, 389
BtsCI	GGATG	4 cut(s)	498, 607, 666, 790
BtsIMutI	CAGTG	3 cut(s)	423, 1188, 1501
Cac8I	GCNNGC	1 cut(s)	718
CaiI	CAGNNNCTG	1 cut(s)	652
CfoI	GCGC	1 cut(s)	954
Cfr13I	GGNCC	2 cut(s)	18, 145
CseI	GACGC	1 cut(s)	1323
CsiI	ACCWGGT	1 cut(s)	20
Csp6I	GTAC	5 cut(s)	365, 1065, 1275, 1349, 1411
CspCI	CAANNNNNGTGG	6 cut(s)	95, 130, 305, 340, 1237, 1272
CviAII	CATG	9 cut(s)	208, 312, 347, 717, 810, 1018, 1234, 1278, 1427
CviQI	GTAC	5 cut(s)	365, 1065, 1275, 1349, 1411
DdeI	CTNAG	3 cut(s)	492, 1060, 1300
DpnI	GATC	7 cut(s)	272, 329, 497, 978, 1098, 1146, 1298
DpnII	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
DraI	TTTAAA	1 cut(s)	837
DrdI	GACNNNNNNGTC	1 cut(s)	25
DseDI	GACNNNNNNGTC	1 cut(s)	25
Eam1104I	CTCTTC	2 cut(s)	597, 1221
EarI	CTCTTC	2 cut(s)	597, 1221
Eco130I	CCWWGG	1 cut(s)	1103
Eco24I	GRGCYC	1 cut(s)	1407
Eco32I	GATATC	1 cut(s)	1162
Eco47I	GGWCC	1 cut(s)	18
Eco88I	CYCGRG	1 cut(s)	261
EcoRI	GAATTC	1 cut(s)	1080
EcoRII	CCWGG	2 cut(s)	20, 708
EcoRV	GATATC	1 cut(s)	1162
EcoT14I	CCWWGG	1 cut(s)	1103
EcoT38I	GRGCYC	1 cut(s)	1407
ErhI	CCWWGG	1 cut(s)	1103
FaeI	CATG	9 cut(s)	211, 315, 350, 720, 813, 1021, 1237, 1281, 1430
FalI	AAGNNNNNCTT	4 cut(s)	495, 527, 1463, 1495
FaqI	GGGAC	3 cut(s)	31, 1229, 1341
FatI	CATG	9 cut(s)	207, 311, 346, 716, 809, 1017, 1233, 1277, 1426
FauI	CCCGC	1 cut(s)	1500
FbaI	TGATCA	1 cut(s)	976
Fnu4HI	GCNGC	3 cut(s)	230, 242, 540
FokI	GGATG	4 cut(s)	485, 614, 653, 797
FriOI	GRGCYC	1 cut(s)	1407
Fsp4HI	GCNGC	3 cut(s)	230, 242, 540
FspBI	CTAG	3 cut(s)	416, 1077, 1397
GlaI	GCGC	1 cut(s)	953
GluI	GCNGC	3 cut(s)	230, 242, 540
GsuI	CTGGAG	1 cut(s)	989
HaeIII	GGCC	2 cut(s)	146, 389
HgaI	GACGC	1 cut(s)	1323
HhaI	GCGC	1 cut(s)	954
Hin1I	GRCGYC	1 cut(s)	1334
Hin1II	CATG	9 cut(s)	211, 315, 350, 720, 813, 1021, 1237, 1281, 1430
Hin6I	GCGC	1 cut(s)	952
HinP1I	GCGC	1 cut(s)	952
HincII	GTYRAC	1 cut(s)	1186
HindII	GTYRAC	1 cut(s)	1186
HinfI	GANTC	3 cut(s)	419, 776, 1238
Hpy166II	GTNNAC	5 cut(s)	30, 367, 1000, 1186, 1283
Hpy188I	TCNGA	4 cut(s)	172, 270, 495, 1096
Hpy188III	TCNNGA	6 cut(s)	43, 63, 623, 968, 1043, 1436
Hpy8I	GTNNAC	5 cut(s)	30, 367, 1000, 1186, 1283
HpyAV	CCTTC	3 cut(s)	624, 942, 1024
HpyCH4III	ACNGT	9 cut(s)	199, 281, 427, 648, 654, 933, 1004, 1252, 1496
HpyCH4IV	ACGT	4 cut(s)	32, 684, 692, 859
HpyCH4V	TGCA	6 cut(s)	232, 350, 443, 644, 961, 1383
HpyF10VI	GCNNNNNNNGC	3 cut(s)	238, 915, 958
HpyF3I	CTNAG	3 cut(s)	492, 1060, 1300
HpySE526I	ACGT	4 cut(s)	32, 684, 692, 859
Hsp92I	GRCGYC	1 cut(s)	1334
Hsp92II	CATG	9 cut(s)	211, 315, 350, 720, 813, 1021, 1237, 1281, 1430
HspAI	GCGC	1 cut(s)	952
Ksp22I	TGATCA	1 cut(s)	976
Kzo9I	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
LguI	GCTCTTC	1 cut(s)	1221
LmnI	GCTCC	2 cut(s)	636, 711
Lsp1109I	GCAGC	2 cut(s)	216, 526
LweI	GCATC	1 cut(s)	1183
MabI	ACCWGGT	1 cut(s)	20
MaeI	CTAG	3 cut(s)	416, 1077, 1397
MaeII	ACGT	4 cut(s)	32, 684, 692, 859
MaeIII	GTNAC	2 cut(s)	199, 648
MalI	GATC	7 cut(s)	272, 329, 497, 978, 1098, 1146, 1298
MbiI	CCGCTC	1 cut(s)	244
MboI	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
MboII	GAAGA	6 cut(s)	279, 327, 584, 1076, 1208, 1483
MflI	RGATCY	1 cut(s)	1144
MhlI	GDGCHC	3 cut(s)	708, 920, 1407
MlyI	GAGTC	1 cut(s)	785
MmeI	TCCRAC	3 cut(s)	15, 579, 694
MseI	TTAA	7 cut(s)	305, 483, 663, 836, 1289, 1307, 1374
MslI	CAYNNNNRTG	3 cut(s)	814, 1365, 1488
MspA1I	CMGCKG	1 cut(s)	229
MspR9I	CCNGG	2 cut(s)	22, 710
MvaI	CCWGG	2 cut(s)	22, 710
MwoI	GCNNNNNNNGC	3 cut(s)	238, 915, 958
NdeII	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
NlaIII	CATG	9 cut(s)	211, 315, 350, 720, 813, 1021, 1237, 1281, 1430
NlaIV	GGNNCC	3 cut(s)	19, 147, 267
NspI	RCATGY	4 cut(s)	350, 720, 813, 1281
PaeI	GCATGC	1 cut(s)	720
PciI	ACATGT	1 cut(s)	1277
PciSI	GCTCTTC	1 cut(s)	1221
PcsI	WCGNNNNNNNCGW	1 cut(s)	856
PfeI	GAWTC	2 cut(s)	419, 1238
PkrI	GCNGC	3 cut(s)	231, 243, 541
PleI	GAGTC	1 cut(s)	784
PpsI	GAGTC	1 cut(s)	784
PscI	ACATGT	1 cut(s)	1277
PshBI	ATTAAT	2 cut(s)	305, 483
Psp1406I	AACGTT	1 cut(s)	684
Psp6I	CCWGG	2 cut(s)	20, 708
PspGI	CCWGG	2 cut(s)	20, 708
PspN4I	GGNNCC	3 cut(s)	19, 147, 267
PspPI	GGNCC	2 cut(s)	18, 145
PstNI	CAGNNNCTG	1 cut(s)	652
PsuI	RGATCY	1 cut(s)	1144
PvuII	CAGCTG	1 cut(s)	229
RsaI	GTAC	5 cut(s)	366, 1066, 1276, 1350, 1412
RsaNI	GTAC	5 cut(s)	365, 1065, 1275, 1349, 1411
RseI	CAYNNNNRTG	3 cut(s)	814, 1365, 1488
SapI	GCTCTTC	1 cut(s)	1221
SaqAI	TTAA	7 cut(s)	305, 483, 663, 836, 1289, 1307, 1374
SatI	GCNGC	3 cut(s)	230, 242, 540
Sau3AI	GATC	7 cut(s)	270, 327, 495, 976, 1096, 1144, 1296
Sau96I	GGNCC	2 cut(s)	18, 145
SchI	GAGTC	1 cut(s)	785
ScrFI	CCNGG	2 cut(s)	22, 710
SduI	GDGCHC	3 cut(s)	708, 920, 1407
SexAI	ACCWGGT	1 cut(s)	20
SfaNI	GCATC	1 cut(s)	1183
SfcI	CTRYAG	1 cut(s)	1071
SinI	GGWCC	1 cut(s)	18
SmiMI	CAYNNNNRTG	3 cut(s)	814, 1365, 1488
SmlI	CTYRAG	1 cut(s)	1406
SmoI	CTYRAG	1 cut(s)	1406
SphI	GCATGC	1 cut(s)	720
SsiI	CCGC	3 cut(s)	242, 1127, 1507
SspI	AATATT	1 cut(s)	487
SspMI	CTAG	3 cut(s)	416, 1077, 1397
StyD4I	CCNGG	2 cut(s)	20, 708
StyI	CCWWGG	1 cut(s)	1103
TaaI	ACNGT	9 cut(s)	199, 281, 427, 648, 654, 933, 1004, 1252, 1496
TaiI	ACGT	4 cut(s)	35, 687, 695, 862
TaqI	TCGA	3 cut(s)	179, 827, 1295
TaqII	GACCGA	1 cut(s)	941
TatI	WGTACW	4 cut(s)	364, 1274, 1348, 1410
TauI	GCSGC	1 cut(s)	244
TfiI	GAWTC	2 cut(s)	419, 1238
Tru1I	TTAA	7 cut(s)	305, 483, 663, 836, 1289, 1307, 1374
Tru9I	TTAA	7 cut(s)	305, 483, 663, 836, 1289, 1307, 1374
TscAI	CASTG	3 cut(s)	430, 1195, 1501
TseI	GCWGC	2 cut(s)	229, 539
TspDTI	ATGAA	6 cut(s)	349, 561, 606, 810, 1006, 1194
TspRI	CASTG	3 cut(s)	430, 1195, 1501
VpaK11BI	GGWCC	1 cut(s)	18
VspI	ATTAAT	2 cut(s)	305, 483
XapI	RAATTY	3 cut(s)	56, 101, 1080
XceI	RCATGY	4 cut(s)	350, 720, 813, 1281
XcmI	CCANNNNNNNNNTGG	2 cut(s)	318, 697
XspI	CTAG	3 cut(s)	416, 1077, 1397

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.