Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001261

Physical Location & Seq

Genomic Coordinates Forward (+)

3869316 .. 3878208

Gene Structure 8893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G002110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGTTGTGAGTCAGTTGCAGCTATGCGTTTTGCCACATCGGTTTCCGAGACTCAACCCCTCTCCTTCTCCTTCTCCTTCTCCTTCTTCTTCTCCAAACAGGAAATATATGCCCTGCCAGTTTTGGTGTATGAAATCTTCATCTTTACATGGACGTAATTTCATAACTCCAAACAGAGGAAAAGGCAAGGTCAGGTCTCAAAAGACAGGCAATAAACTAGATTCCACTATGTACATAAATGTTGAAGATGACTCGGACACCGAACTTCTTTGGATAGAACAAGTAAAAAGTTCTCAGATTCACCTAGCTAAAGTCTTGATGGAATTGACGTGCTTATATATTCCACTTAGACTGGCAAAGCTAAATGTCGTTGATACTTTCATTAAGATTGTCCAAGAAAGACTTCTCTGCACTGTTCAAAATTTAGGGGCCGCCACCTTGCCTTTTGCCTGTGCTTCTAATTCTCTGAATAAACCACTGCCTCTTGGATTGGATGTGTCGTTGCCATCATTTCAAGATGTTAGATGGAGCTTTCAACGATTATTGTATCTTTTTAACGTCCAACTTGAGAAAAATGTTGCTACGTTCTTCATAGTGCTCCTGGTAGCATGTTTCTCATTTGTTATCATTGGTGGTTTCCTATTCTTCAAGTTTAGGGGTAGTAAAGAATCTTTGGAGGACTGCTTCTGGGAAGCTTGGGCATGCCTATGTTCATCATCGACACATCTAAAGCAAAGAACACGTATTGAACGTGTTATTGGTTTCGTTCTTGCTATATGGGGCATATTATTTTACTCCCGCTTATTGAGCACAATGACTGAACAGTTCCGGAGTAATATGTACAAGCTCAGAGAAGGGGCACAAATGCAAGTACTGGAGACTGATCACATCATTATTTGTGGAGTGAACAGTCATCTTTCTTTCATACTGAAGCAAATAAACAAGTATCATGAATTTGCTGTCCGCTTAGGTACTGCTACAGCTAGGAAGCAAAGAATTCTTCTTATGTCTGACTTACCAAGAAAGCAGATGGACAAGTTAGCTGACCTTATTGCCAAAGATTTTAACCATATCGATATCCTTACAAAAAGTTGTAGCCTGAGCTTAACAAAATCATATGAAAGAGCCGCTGCCAACAAGGCACGTGCCATAATTATACTGCCAACAAAGGCAGATCGGTATGAAGTTGATACTGATGCCTTTCTATCCGTTCTGGCTCTTCAACCTATTCCTAAGATGGAGTCCGTCCCCACTATTGTTGAGGTTTCAAGTTCAAATACATGCGAGCTTTTGAAGTCAATCTCAGGGTTGAAAGTAGCGCCTGTGGAGAACGTTTCATCCAAATTATTTGTTCAATGCTCTCGTCAAAAAGGACTCATAAAGATCTATAGACACTTGCTTAACTATCGAAAAAATGTGTTCAATCTTTGGAATTCTCCTAGTCTAGCAGGGCTAAAGTATAGGCAATTAGGGCCTGGATTTCAAGATGCAGTTGTTTGTGGGCTTTATAGAAACGGGAAGGTATACTTCCACCCAAATGATGATGAGATCTTGCAGGAAACTGACAAAGTATTGTTCATTGCACCTGTCAGTGGGGGTAAGAAACCTCCTATTACTTATTCGAATGTTAAAGAAATAAGCGATGCCAATCGAAGTTTGGAGGATGTAGAAAAGGACAGTGATGCTCGATCAGATGCCTTAAAATTAAGGAAAGAAAAAACACGTCCCAAGAGATGGGGCTCCAAGGCATCAGACGGGACTCTAGGACCAACAGAATTTATACTTCTGCTTGGATGGCGGCCAGACGTCGTTGAGATGATTGAAGAATATGACAATTATCTCGGACCTGGGAGTGTCGTGGAGATATTGTCAGATGTACCGTTAGATGACAGAAATAAGGCAAGAAAAGGTGCCGGTCAAGCCAAACTAAAACATGTGCAGGTTTCACATAGGATTGGAAACCCAATGAACTTTGACACTTTACAAGAAACCATAAGCAATATCCAACATTCTTTGAAGAATGAAGATATTCCCTTATCCATTGTTGTAATATCCGATAGAGAATGGCTTCTTGGAGATCCAAACAGGGCAGACAAGCAATCCGCCTATTCCCTTCTTCTTGCAGAAAATATTTGCAACAAACTTGGCGTAAAGGTGCAGAATCTAGTTGCGGAAATCGTGGACTCGAAATTGGGGAAACAAATCACAAGAATAAAGCCATCCCTGACTTATATTGCCGCGGAGGAAGTAATGAGCCTCGTAACAGCTCAAGTTGCTGAGGACAGTGAACTAAATGAGGTGTGGAAAGACATTCTGGATGCAGACGGTGATGAAATATATGTGAAGGATATCAAACTCTATATGAAAGAAGGAGAGACTCCATCCTTCACGGAGCTTGCTGAAAGAGCTCACCTACGGAAGGAAGTTGCCATAGGTTATGTGAAAAACAATAAGAAGGTTGTTAATCCAGTTCCCAAGTCAGAACCCCTCTCCCTTGAGTTGACAGATTCGTTAATAGTTATATCTGAACTTGAAGGAGAACAACCGATCATTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Castor_Poll_mid

Protein Analysis

852

Amino Acids

96.02

Weight (kDa)

8.73

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Castor_Poll_mid	PF06241	443 - 543	3.1e-37	Castor and Pollux, part of voltage-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001261)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02940 AT5G02940 AT5G43745
fragaria_vesca	FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230
malus_domestica	MD11G1069300.v1.1 MD13G1204000.v1.1 MD16G1206000.v1.1
prunus_persica	Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1
pyrus_communis	pycom11g05780 pycom13g17720
rosa_chinensis	RchiOBHm_Chr4g0389551 RchiOBHm_Chr4g0389561 RchiOBHm_Chr4g0389571 RchiOBHm_Chr4g0389581
rosa_laevigata	RLG00000009970 RLG00000009971 RLG00000009972
rosa_multiflora	Rmu_co8238337.1_g000001 Rmu_co8398985.1_g000001 Rmu_sc0010540.1_g000001 Rmu_ssc0000478.1_g000001 Rmu_ssc0000478.1_g000002 Rmu_ssc0000478.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335510 Rroxscaffold_5G00335520 Rroxscaffold_5G00335530
rosa_rugosa	Rorug03G0324000
rosa_samantha	Rh2BG306500 Rh2CG284600 Rh4AG028300 Rh4AG028400 Rh4AG028500 Rh4BG021500 Rh4BG021600 Rh4BG021700 Rh4CG030500 Rh4CG030700 Rh4DG022100 Rh4DG022200 Rh4DG022300
rosa_wichuraiana	Rw4G002110 Rw4G002120 Rw4G002130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1809
Acc36I	ACCTGC	1 cut(s)	1930
AccB1I	GGYRCC	1 cut(s)	1910
AccB7I	CCANNNNNTGG	1 cut(s)	1734
AccI	GTMKAC	1 cut(s)	1524
AccII	CGCG	1 cut(s)	2240
AccIII	TCCGGA	1 cut(s)	828
AciI	CCGC	9 cut(s)	432, 799, 964, 1128, 1798, 2103, 2171, 2238, 2240
AclI	AACGTT	1 cut(s)	1332
AclWI	GGATC	1 cut(s)	2072
AcoI	YGGCCR	1 cut(s)	1799
AcsI	RAATTY	5 cut(s)	421, 953, 996, 1432, 1775
AcuI	CTGAAG	1 cut(s)	950
AcvI	CACGTG	1 cut(s)	1145
AcyI	GRCGYC	1 cut(s)	1806
AfaI	GTAC	5 cut(s)	233, 842, 873, 973, 1878
AfiI	CCNNNNNNNGG	4 cut(s)	176, 1592, 1734, 2419
AflIII	ACRYGT	4 cut(s)	740, 751, 1721, 1933
AjiI	CACGTC	2 cut(s)	330, 1724
AjnI	CCWGG	3 cut(s)	600, 1474, 1846
AjuI	GAANNNNNNNTTGG	6 cut(s)	387, 419, 741, 773, 2055, 2087
AloI	GAACNNNNNNTCC	2 cut(s)	1322, 1354
Alw21I	GWGCWC	3 cut(s)	600, 812, 2410
Alw26I	GTCTC	4 cut(s)	43, 201, 872, 2369
AlwI	GGATC	1 cut(s)	2072
Aor13HI	TCCGGA	1 cut(s)	828
AoxI	GGCC	3 cut(s)	429, 1472, 1799
ApeKI	GCWGC	2 cut(s)	19, 1130
ApoI	RAATTY	5 cut(s)	421, 953, 996, 1432, 1775
Asp700I	GAANNNNTTC	3 cut(s)	402, 2028, 2067
AspLEI	GCGC	1 cut(s)	1321
AspS9I	GGNCC	4 cut(s)	429, 1472, 1766, 1844
AsuHPI	GGTGA	3 cut(s)	293, 2339, 2402
AsuII	TTCGAA	1 cut(s)	1622
AvaII	GGWCC	2 cut(s)	1766, 1844
BaeGI	GKGCMC	1 cut(s)	862
BanI	GGYRCC	1 cut(s)	1910
BanII	GRGCYC	2 cut(s)	1742, 2410
BarI	GAAGNNNNNNTAC	2 cut(s)	121, 153
BbrPI	CACGTG	1 cut(s)	1145
Bbv12I	GWGCWC	3 cut(s)	600, 812, 2410
BbvCI	CCTCAGC	1 cut(s)	2277
BbvI	GCAGC	2 cut(s)	31, 1117
BccI	CCATC	9 cut(s)	313, 514, 519, 1024, 1231, 1728, 1788, 2227, 2389
BciT130I	CCWGG	3 cut(s)	602, 1476, 1848
BclI	TGATCA	1 cut(s)	883
BcoDI	GTCTC	4 cut(s)	43, 201, 872, 2369
BfaI	CTAG	7 cut(s)	218, 305, 984, 1440, 1445, 1763, 2165
BfmI	CTRYAG	2 cut(s)	978, 1387
BfoI	RGCGCY	1 cut(s)	1322
BfuAI	ACCTGC	1 cut(s)	1930
BglI	GCCNNNNNGGC	1 cut(s)	1139
BglII	AGATCT	2 cut(s)	1383, 1548
BisI	GCNGC	6 cut(s)	20, 432, 1128, 1131, 1799, 2238
BlsI	GCNGC	6 cut(s)	21, 433, 1129, 1132, 1800, 2239
BmcAI	AGTACT	1 cut(s)	873
Bme1390I	CCNGG	3 cut(s)	602, 1476, 1848
Bme18I	GGWCC	2 cut(s)	1766, 1844
BmgBI	CACGTC	2 cut(s)	330, 1724
BmgT120I	GGNCC	4 cut(s)	429, 1472, 1766, 1844
BmiI	GGNNCC	3 cut(s)	430, 1741, 1912
BmrFI	CCNGG	3 cut(s)	602, 1476, 1848
BmsI	GCATC	7 cut(s)	1186, 1477, 1633, 1672, 1684, 1757, 2308
BpmI	CTGGAG	1 cut(s)	896
Bpu10I	CCTNAGC	3 cut(s)	967, 1100, 2277
Bpu14I	TTCGAA	1 cut(s)	1622
BpuEI	CTTGAG	3 cut(s)	587, 2253, 2516
Bsa29I	ATCGAT	1 cut(s)	1074
BsaAI	YACGTR	2 cut(s)	743, 1145
BsaBI	GATNNNNATC	1 cut(s)	1647
BsaHI	GRCGYC	1 cut(s)	1806
BsaI	GGTCTC	1 cut(s)	201
BsaJI	CCNNGG	3 cut(s)	1743, 1847, 2238
BsaWI	WCCGGW	1 cut(s)	828
BsaXI	ACNNNNNCTCC	2 cut(s)	1853, 1883
Bsc4I	CCNNNNNNNGG	4 cut(s)	176, 1592, 1734, 2419
Bse118I	RCCGGY	1 cut(s)	1913
Bse1I	ACTGG	4 cut(s)	118, 357, 879, 2468
Bse3DI	GCAATG	1 cut(s)	1578
Bse8I	GATNNNNATC	1 cut(s)	1647
BseAI	TCCGGA	1 cut(s)	828
BseBI	CCWGG	3 cut(s)	602, 1476, 1848
BseCI	ATCGAT	1 cut(s)	1074
BseDI	CCNNGG	3 cut(s)	1743, 1847, 2238
BseGI	GGATG	7 cut(s)	499, 1337, 1669, 1799, 2219, 2323, 2381
BseJI	GATNNNNATC	1 cut(s)	1647
BseLI	CCNNNNNNNGG	4 cut(s)	176, 1592, 1734, 2419
BseMI	GCAATG	1 cut(s)	1578
BseMII	CTCAG	5 cut(s)	308, 862, 1091, 1317, 2268
BseNI	ACTGG	4 cut(s)	118, 357, 879, 2468
BseSI	GKGCMC	1 cut(s)	862
BseXI	GCAGC	2 cut(s)	31, 1117
BsgI	GTGCAG	3 cut(s)	394, 1958, 2177
Bsh1236I	CGCG	1 cut(s)	2240
BshFI	GGCC	3 cut(s)	431, 1474, 1801
BshNI	GGYRCC	1 cut(s)	1910
BshVI	ATCGAT	1 cut(s)	1074
BsiHKAI	GWGCWC	3 cut(s)	600, 812, 2410
BsiSI	CCGG	2 cut(s)	829, 1914
BslFI	GGGAC	3 cut(s)	1232, 1710, 1771
BslI	CCNNNNNNNGG	4 cut(s)	176, 1592, 1734, 2419
BsmAI	GTCTC	4 cut(s)	43, 201, 872, 2369
BsmFI	GGGAC	3 cut(s)	1232, 1710, 1771
BsnI	GGCC	3 cut(s)	431, 1474, 1801
Bso31I	GGTCTC	1 cut(s)	201
Bsp119I	TTCGAA	1 cut(s)	1622
Bsp1286I	GDGCHC	5 cut(s)	600, 812, 862, 1742, 2410
Bsp13I	TCCGGA	1 cut(s)	828
Bsp1407I	TGTACA	2 cut(s)	231, 840
Bsp143I	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
BspACI	CCGC	9 cut(s)	432, 799, 964, 1128, 1798, 2103, 2171, 2238, 2240
BspANI	GGCC	3 cut(s)	431, 1474, 1801
BspCNI	CTCAG	5 cut(s)	307, 861, 1092, 1316, 2269
BspDI	ATCGAT	1 cut(s)	1074
BspEI	TCCGGA	1 cut(s)	828
BspFNI	CGCG	1 cut(s)	2240
BspHI	TCATGA	1 cut(s)	949
BspLI	GGNNCC	3 cut(s)	430, 1741, 1912
BspMI	ACCTGC	1 cut(s)	1930
BspPI	GGATC	1 cut(s)	2072
BspQI	GCTCTTC	1 cut(s)	1224
BspT104I	TTCGAA	1 cut(s)	1622
BspT107I	GGYRCC	1 cut(s)	1910
BspTNI	GGTCTC	1 cut(s)	201
BsrDI	GCAATG	1 cut(s)	1578
BsrFI	RCCGGY	1 cut(s)	1913
BsrGI	TGTACA	2 cut(s)	231, 840
BsrI	ACTGG	4 cut(s)	118, 357, 879, 2468
BssAI	RCCGGY	1 cut(s)	1913
BssECI	CCNNGG	3 cut(s)	1743, 1847, 2238
BssMI	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
BssNAI	GTATAC	1 cut(s)	1525
BssNI	GRCGYC	1 cut(s)	1806
BssT1I	CCWWGG	1 cut(s)	1743
Bst1107I	GTATAC	1 cut(s)	1525
Bst2UI	CCWGG	3 cut(s)	602, 1476, 1848
Bst4CI	ACNGT	7 cut(s)	415, 825, 911, 1679, 1881, 2285, 2327
Bst6I	CTCTTC	1 cut(s)	1224
BstACI	GRCGYC	1 cut(s)	1806
BstAUI	TGTACA	2 cut(s)	231, 840
BstBAI	YACGTR	2 cut(s)	743, 1145
BstBI	TTCGAA	1 cut(s)	1622
BstC8I	GCNNGC	3 cut(s)	703, 1286, 2397
BstDEI	CTNAG	8 cut(s)	294, 347, 848, 967, 1100, 1233, 1303, 2277
BstDSI	CCRYGG	1 cut(s)	2238
BstENI	CCTNNNNNAGG	1 cut(s)	2417
BstF5I	GGATG	7 cut(s)	499, 1337, 1669, 1799, 2219, 2323, 2381
BstFNI	CGCG	1 cut(s)	2240
BstH2I	RGCGCY	1 cut(s)	1322
BstHHI	GCGC	1 cut(s)	1321
BstKTI	GATC	7 cut(s)	886, 1177, 1386, 1551, 1691, 2080, 2550
BstMAI	GTCTC	4 cut(s)	43, 201, 872, 2369
BstMBI	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
BstMWI	GCNNNNNNNGC	7 cut(s)	807, 1139, 1471, 1795, 1919, 2273, 2405
BstNI	CCWGG	3 cut(s)	602, 1476, 1848
BstNSI	RCATGY	4 cut(s)	612, 705, 1284, 1937
BstSCI	CCNGG	3 cut(s)	600, 1474, 1846
BstSFI	CTRYAG	2 cut(s)	978, 1387
BstSLI	GKGCMC	1 cut(s)	862
BstUI	CGCG	1 cut(s)	2240
BstV1I	GCAGC	2 cut(s)	31, 1117
BstX2I	RGATCY	3 cut(s)	1383, 1548, 2077
BstYI	RGATCY	3 cut(s)	1383, 1548, 2077
BstZ17I	GTATAC	1 cut(s)	1525
Bsu15I	ATCGAT	1 cut(s)	1074
BsuRI	GGCC	3 cut(s)	431, 1474, 1801
BsuTUI	ATCGAT	1 cut(s)	1074
BtgI	CCRYGG	1 cut(s)	2238
BtgZI	GCGATG	1 cut(s)	1656
BtrI	CACGTC	2 cut(s)	330, 1724
BtsCI	GGATG	7 cut(s)	499, 1337, 1669, 1799, 2219, 2323, 2381
BtsI	GCAGTG	1 cut(s)	476
BtsIMutI	CAGTG	5 cut(s)	411, 476, 1597, 1684, 2290
BveI	ACCTGC	1 cut(s)	1930
Cac8I	GCNNGC	3 cut(s)	703, 1286, 2397
CciI	TCATGA	1 cut(s)	949
CfoI	GCGC	1 cut(s)	1321
Cfr10I	RCCGGY	1 cut(s)	1913
Cfr13I	GGNCC	4 cut(s)	429, 1472, 1766, 1844
Cfr42I	CCGCGG	1 cut(s)	2241
ClaI	ATCGAT	1 cut(s)	1074
Csp6I	GTAC	5 cut(s)	232, 841, 872, 972, 1877
CspCI	CAANNNNNGTGG	2 cut(s)	1240, 1275
CviAII	CATG	6 cut(s)	149, 609, 702, 950, 1281, 1934
CviQI	GTAC	5 cut(s)	232, 841, 872, 972, 1877
DdeI	CTNAG	8 cut(s)	294, 347, 848, 967, 1100, 1233, 1303, 2277
DpnI	GATC	7 cut(s)	885, 1176, 1385, 1550, 1690, 2079, 2549
DpnII	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
EaeI	YGGCCR	1 cut(s)	1799
Eam1104I	CTCTTC	1 cut(s)	1224
EarI	CTCTTC	1 cut(s)	1224
EciI	GGCGGA	1 cut(s)	2092
Ecl136II	GAGCTC	1 cut(s)	2408
Eco130I	CCWWGG	1 cut(s)	1743
Eco24I	GRGCYC	2 cut(s)	1742, 2410
Eco31I	GGTCTC	1 cut(s)	201
Eco32I	GATATC	2 cut(s)	1078, 2350
Eco47I	GGWCC	2 cut(s)	1766, 1844
Eco53kI	GAGCTC	1 cut(s)	2408
Eco57I	CTGAAG	1 cut(s)	950
Eco72I	CACGTG	1 cut(s)	1145
EcoICRI	GAGCTC	1 cut(s)	2408
EcoNI	CCTNNNNNAGG	1 cut(s)	2417
EcoO109I	RGGNCCY	1 cut(s)	1472
EcoRI	GAATTC	2 cut(s)	996, 1432
EcoRII	CCWGG	3 cut(s)	600, 1474, 1846
EcoRV	GATATC	2 cut(s)	1078, 2350
EcoT14I	CCWWGG	1 cut(s)	1743
EcoT38I	GRGCYC	2 cut(s)	1742, 2410
ErhI	CCWWGG	1 cut(s)	1743
FaeI	CATG	6 cut(s)	152, 612, 705, 953, 1284, 1937
FalI	AAGNNNNNCTT	4 cut(s)	387, 419, 1511, 1543
FaqI	GGGAC	3 cut(s)	1232, 1710, 1771
FatI	CATG	6 cut(s)	148, 608, 701, 949, 1280, 1933
FauI	CCCGC	1 cut(s)	806
FauNDI	CATATG	1 cut(s)	1117
FbaI	TGATCA	1 cut(s)	883
FblI	GTMKAC	1 cut(s)	1524
Fnu4HI	GCNGC	6 cut(s)	20, 432, 1128, 1131, 1799, 2238
FokI	GGATG	7 cut(s)	506, 1324, 1676, 1806, 2206, 2330, 2368
FriOI	GRGCYC	2 cut(s)	1742, 2410
Fsp4HI	GCNGC	6 cut(s)	20, 432, 1128, 1131, 1799, 2238
FspBI	CTAG	7 cut(s)	218, 305, 984, 1440, 1445, 1763, 2165
GlaI	GCGC	1 cut(s)	1320
GluI	GCNGC	6 cut(s)	20, 432, 1128, 1131, 1799, 2238
GsuI	CTGGAG	1 cut(s)	896
HaeII	RGCGCY	1 cut(s)	1322
HaeIII	GGCC	3 cut(s)	431, 1474, 1801
HapII	CCGG	2 cut(s)	829, 1914
HhaI	GCGC	1 cut(s)	1321
Hin1I	GRCGYC	1 cut(s)	1806
Hin1II	CATG	6 cut(s)	152, 612, 705, 953, 1284, 1937
Hin6I	GCGC	1 cut(s)	1319
HinP1I	GCGC	1 cut(s)	1319
HincII	GTYRAC	1 cut(s)	2502
HindII	GTYRAC	1 cut(s)	2502
HindIII	AAGCTT	1 cut(s)	693
HpaII	CCGG	2 cut(s)	829, 1914
HphI	GGTGA	3 cut(s)	293, 2339, 2402
Hpy166II	GTNNAC	5 cut(s)	907, 1525, 2182, 2288, 2502
Hpy188III	TCNNGA	6 cut(s)	316, 515, 829, 950, 1484, 2315
Hpy8I	GTNNAC	5 cut(s)	907, 1525, 2182, 2288, 2502
Hpy99I	CGWCG	1 cut(s)	1811
HpyCH4III	ACNGT	7 cut(s)	415, 825, 911, 1679, 1881, 2285, 2327
HpyF10VI	GCNNNNNNNGC	7 cut(s)	807, 1139, 1471, 1795, 1919, 2273, 2405
HpyF3I	CTNAG	8 cut(s)	294, 347, 848, 967, 1100, 1233, 1303, 2277
Hsp92I	GRCGYC	1 cut(s)	1806
Hsp92II	CATG	6 cut(s)	152, 612, 705, 953, 1284, 1937
HspAI	GCGC	1 cut(s)	1319
Kpn2I	TCCGGA	1 cut(s)	828
Ksp22I	TGATCA	1 cut(s)	883
KspI	CCGCGG	1 cut(s)	2241
Kzo9I	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
LguI	GCTCTTC	1 cut(s)	1224
LmnI	GCTCC	4 cut(s)	528, 603, 1745, 2392
Lsp1109I	GCAGC	2 cut(s)	31, 1117
LweI	GCATC	7 cut(s)	1186, 1477, 1633, 1672, 1684, 1757, 2308
MaeI	CTAG	7 cut(s)	218, 305, 984, 1440, 1445, 1763, 2165
MaeIII	GTNAC	1 cut(s)	2260
MalI	GATC	7 cut(s)	885, 1176, 1385, 1550, 1690, 2079, 2549
MboI	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
MflI	RGATCY	3 cut(s)	1383, 1548, 2077
MhlI	GDGCHC	5 cut(s)	600, 812, 862, 1742, 2410
MlyI	GAGTC	8 cut(s)	19, 45, 245, 1250, 1368, 1753, 2177, 2371
MmeI	TCCRAC	2 cut(s)	586, 2029
MroI	TCCGGA	1 cut(s)	828
MroXI	GAANNNNTTC	3 cut(s)	402, 2028, 2067
MslI	CAYNNNNRTG	2 cut(s)	706, 1536
MspA1I	CMGCKG	2 cut(s)	1130, 2240
MspI	CCGG	2 cut(s)	829, 1914
MspR9I	CCNGG	3 cut(s)	602, 1476, 1848
MvaI	CCWGG	3 cut(s)	602, 1476, 1848
MvnI	CGCG	1 cut(s)	2240
MwoI	GCNNNNNNNGC	7 cut(s)	807, 1139, 1471, 1795, 1919, 2273, 2405
NdeI	CATATG	1 cut(s)	1117
NdeII	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
NlaIII	CATG	6 cut(s)	152, 612, 705, 953, 1284, 1937
NlaIV	GGNNCC	3 cut(s)	430, 1741, 1912
NspI	RCATGY	4 cut(s)	612, 705, 1284, 1937
NspV	TTCGAA	1 cut(s)	1622
PaeI	GCATGC	1 cut(s)	705
PagI	TCATGA	1 cut(s)	949
PciI	ACATGT	1 cut(s)	1933
PciSI	GCTCTTC	1 cut(s)	1224
PcsI	WCGNNNNNNNCGW	1 cut(s)	748
PdmI	GAANNNNTTC	3 cut(s)	402, 2028, 2067
PfeI	GAWTC	5 cut(s)	221, 298, 668, 2161, 2507
PflMI	CCANNNNNTGG	1 cut(s)	1734
PkrI	GCNGC	6 cut(s)	21, 433, 1129, 1132, 1800, 2239
PleI	GAGTC	8 cut(s)	18, 45, 245, 1249, 1368, 1753, 2177, 2371
PmaCI	CACGTG	1 cut(s)	1145
PmlI	CACGTG	1 cut(s)	1145
PpsI	GAGTC	8 cut(s)	18, 45, 245, 1249, 1368, 1753, 2177, 2371
Ppu21I	YACGTR	2 cut(s)	743, 1145
PscI	ACATGT	1 cut(s)	1933
Psp124BI	GAGCTC	1 cut(s)	2410
Psp1406I	AACGTT	1 cut(s)	1332
Psp6I	CCWGG	3 cut(s)	600, 1474, 1846
PspCI	CACGTG	1 cut(s)	1145
PspGI	CCWGG	3 cut(s)	600, 1474, 1846
PspN4I	GGNNCC	3 cut(s)	430, 1741, 1912
PspPI	GGNCC	4 cut(s)	429, 1472, 1766, 1844
PsuI	RGATCY	3 cut(s)	1383, 1548, 2077
RsaI	GTAC	5 cut(s)	233, 842, 873, 973, 1878
RsaNI	GTAC	5 cut(s)	232, 841, 872, 972, 1877
RseI	CAYNNNNRTG	2 cut(s)	706, 1536
SacI	GAGCTC	1 cut(s)	2410
SacII	CCGCGG	1 cut(s)	2241
SapI	GCTCTTC	1 cut(s)	1224
SatI	GCNGC	6 cut(s)	20, 432, 1128, 1131, 1799, 2238
Sau3AI	GATC	7 cut(s)	883, 1174, 1383, 1548, 1688, 2077, 2547
Sau96I	GGNCC	4 cut(s)	429, 1472, 1766, 1844
ScaI	AGTACT	1 cut(s)	873
SchI	GAGTC	8 cut(s)	19, 45, 245, 1250, 1368, 1753, 2177, 2371
ScrFI	CCNGG	3 cut(s)	602, 1476, 1848
SduI	GDGCHC	5 cut(s)	600, 812, 862, 1742, 2410
SfaNI	GCATC	7 cut(s)	1186, 1477, 1633, 1672, 1684, 1757, 2308
SfcI	CTRYAG	2 cut(s)	978, 1387
Sfr303I	CCGCGG	1 cut(s)	2241
SfuI	TTCGAA	1 cut(s)	1622
SgrBI	CCGCGG	1 cut(s)	2241
SinI	GGWCC	2 cut(s)	1766, 1844
SmiMI	CAYNNNNRTG	2 cut(s)	706, 1536
SmlI	CTYRAG	3 cut(s)	566, 2268, 2495
SmoI	CTYRAG	3 cut(s)	566, 2268, 2495
SphI	GCATGC	1 cut(s)	705
SsiI	CCGC	9 cut(s)	432, 799, 964, 1128, 1798, 2103, 2171, 2238, 2240
SspI	AATATT	1 cut(s)	2131
SspMI	CTAG	7 cut(s)	218, 305, 984, 1440, 1445, 1763, 2165
SstI	GAGCTC	1 cut(s)	2410
StyD4I	CCNGG	3 cut(s)	600, 1474, 1846
StyI	CCWWGG	1 cut(s)	1743
TaaI	ACNGT	7 cut(s)	415, 825, 911, 1679, 1881, 2285, 2327
TaqI	TCGA	7 cut(s)	719, 1074, 1408, 1622, 1651, 1687, 2186
TatI	WGTACW	3 cut(s)	231, 840, 871
TauI	GCSGC	4 cut(s)	434, 1130, 1801, 2240
TfiI	GAWTC	5 cut(s)	221, 298, 668, 2161, 2507
TscAI	CASTG	5 cut(s)	418, 483, 1597, 1684, 2290
TseI	GCWGC	2 cut(s)	19, 1130
TspGWI	ACGGA	4 cut(s)	1198, 1234, 2405, 2431
TspRI	CASTG	5 cut(s)	418, 483, 1597, 1684, 2290
Van91I	CCANNNNNTGG	1 cut(s)	1734
VpaK11BI	GGWCC	2 cut(s)	1766, 1844
XagI	CCTNNNNNAGG	1 cut(s)	2417
XapI	RAATTY	5 cut(s)	421, 953, 996, 1432, 1775
XceI	RCATGY	4 cut(s)	612, 705, 1284, 1937
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1654
XmiI	GTMKAC	1 cut(s)	1524
XmnI	GAANNNNTTC	3 cut(s)	402, 2028, 2067
XspI	CTAG	7 cut(s)	218, 305, 984, 1440, 1445, 1763, 2165
ZraI	GACGTC	1 cut(s)	1807
ZrmI	AGTACT	1 cut(s)	873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.