Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001261

Physical Location & Seq

Genomic Coordinates Forward (+)

19035158 .. 19043745

Gene Structure 8588 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1206000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2556 bp

ATGGTTCTTCCGAGTAGTCAGCTGCACAGCTTGCAGCTCTGCGTTTTGCCTTCTCGGGTTCCCGTCTCCAACCGTTTTTCTTCCCCAAAAAGGAAATTAATGCCATGTCACTTTTGGTGGATCAAATCTCCGTCTTTACATGCATGTAGTTTCAACAGAGGAAAATGTGAGGCCAGTTCTCAAAGGGCAGAAAATAAGTTAGATTCCACAGTGTATATAAATGTTGCAGACGATTCGAATGACAAACTCTTTAGTGCAGACCCAATTAATATTTCTCAGGTTCACCTGACTAAAGTCACGATGGGATTGGTGTCCTTATATTTCCCAATTAGACTGGTGCAGTCAAGTATTTTTAATACTTTCATCAAGATTGTCCAAGCGAAACTTCCCTATATCATTCAAACTTTCGGGGCTGCCACCTTGCCCTTTGCCTGTGTTTCTAATTCTTTGAATAAACCTGTGCCTCTTCGGTTGGATGTGTCTTTTCCTTCACTTCAAGATATTAGATGGAGCTTTGCACGGTTACTGTATCTATTTAACATCCAACTTGAGAAAAATGTTGCCACGTTCTTTCTGGTGCTCCTGGTAGCATGCTTCTCATTTGTTGTCATTGGTGGTTTCCTATTCTACAACTTTAGGGGTAGTAAGGAGTCTTTGGAGGACTGCTTCTGGGAGGCTTGGGCATGTCTGTGTTCATCATCAACACATCTAAAACAAAGAACACGCATTGAACGTGTTATTGGTTTTGTTCTTACAATATGGGGCATATTATTTTACTCTCGCTTGTTGACCACAATGACTGAACAGTTCAGGAACAATATGTACAGGCTCAGAGAGGGTGCACAAATACAAGTTCTGGAGACTGATCATATCATTATTTGTGGAGTGAACAGTCATCTTCAGTTCATACTGAAGCAACTAAACAAGTATCATGAATTTGCTGTCCGCTTAGGTACTGCTACAGCTAGGAGGCAGAAAATTCTTCTTATGTCCGATCTCCCAAGAAAGCAGATGGACAAGCTCGCGGACAATTTAGCCAAAGATCTTATTCATATTGATATCCTTTCAAAGAGTTGCAGCCTTAGTTTAACAAAATCATTTGAAAGAGCCGCTGCCAACAAGGCACGTGCAATAATTATACTGCCAACGAAGGGTGACCGGTATGAAGTTGACACCGATGCTTTTTTGTCTGTTCTGGCTCTTCAACCTATTCCTAACATGGAATCTGTCCCCACAATTGTTGAGGTTTCAAGTTCCAATACGTGTGAGCTCTTAAAGTCGATCTCAGGGTTAAAAGTAGAGCCTGTAGAAAATGGTGCATCAAAATTATTTGTTCAATGCTCTCGTCAAAAGGGACTCATAAAGATCTACAGGCACTTGCTTAATTATCAAAAAAATGTATTTAATCTTTGCAGTTTCCCAAGTCTAGCGGGGATCAAGTATAGGAGGTTACGACATGGGTTTCAAGAGGCAGTTGTTTGTGGGCTTTATAGGAACGGGAAGATAGACTTCCACCCCAATGACAGTGAAATCTTGCGGGAAACTGACAAAGTATTATTCATTGCACCTGTTAATGGGAGTAAGACACCAGATGTTACTTATTCGAATGTTGTCAAAGAAATAGGCGCTGATCAAAGTTTGGATGATCTAGAAACAAACGGTGGTACTCACCCGCATTCCTTGCAACTGGTGAAAACACGACTTGAGAACATTGTCAGGCGTCCCAAGAAACCAGGCTCTAAGGCTTCAGATTATAATCTGGGACCAAAAGAATTTATACTTCTGCTTGGATGGCGCCCTGACATCGTAGAGATGATTGAAGAATATGACAATTATCTTGGACCTGGCAGTGTCGTGGAAATTCTGTCAGATGTACCGTTAGATGACAGAACAAGGACAAGACAAGTTTCTGAACACGGAAAACTCAAACACATCAAGGTTTCTCATCGGATTGGAAATCCATTGAACTTTGAAACCTTACAAGATACCATCAAGCATATCCATAATTCATTGAAGAACGAAGATGTTCCCTTGTCGGTGGTTGTAACATCCGATAGAGAATGGCTTCTCGGAGATCCAACCAGGGCAGACAAGCAATCTGCGTATTCTCTTCTTCTTGCCGAAAATATTTGCAACAAACTTGGTGTAAAGGTACAGAATCTTGTTGCAGAGATCGTGGACTCGAATTTGGGCAAACAAATCATGAGAATTAAGCCGTCCCTGACTTACATTGCCGCAGAGGAAGTAATGAGCCTCGTAACAGCCCAAGTTGCTGAGAACAGTGAACTAAATGAAGTGTGGAAAGACATTCTTAACGCCGAGGGTGATGAAATATACATAAAGGATATCAGCCTCTATATTAAAGATGGAGAGAACCCTTCGTTCTTTGAGCTTTCTGAAAGAGCACAACTGCGGAAAGAAGTGGCGATAGGTTATGTGAAAAACAACAAGAAGGTTATCAATCCAGTTCCCAAGTCCGAACCCCTCTCCCTTGAGTTGACAGATTCGTTGATAGTTATATCTGAACTCGAAGGAGAACAACCTATCGTTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Castor_Poll_mid

Protein Analysis

852

Amino Acids

96.1

Weight (kDa)

8.63

Isoelectric Point (pI)

42.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Castor_Poll_mid	PF06241	440 - 536	1.4e-38	Castor and Pollux, part of voltage-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001261)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02940 AT5G02940 AT5G43745
fragaria_vesca	FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230 FvH4_4g02230
malus_domestica	MD11G1069300.v1.1 MD13G1204000.v1.1 MD16G1206000.v1.1
prunus_persica	Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1 Prupe.1G027100_v2.0.a1
pyrus_communis	pycom11g05780 pycom13g17720
rosa_chinensis	RchiOBHm_Chr4g0389551 RchiOBHm_Chr4g0389561 RchiOBHm_Chr4g0389571 RchiOBHm_Chr4g0389581
rosa_laevigata	RLG00000009970 RLG00000009971 RLG00000009972
rosa_multiflora	Rmu_co8238337.1_g000001 Rmu_co8398985.1_g000001 Rmu_sc0010540.1_g000001 Rmu_ssc0000478.1_g000001 Rmu_ssc0000478.1_g000002 Rmu_ssc0000478.1_g000006
rosa_roxburghii	Rroxscaffold_5G00335510 Rroxscaffold_5G00335520 Rroxscaffold_5G00335530
rosa_rugosa	Rorug03G0324000
rosa_samantha	Rh2BG306500 Rh2CG284600 Rh4AG028300 Rh4AG028400 Rh4AG028500 Rh4BG021500 Rh4BG021600 Rh4BG021700 Rh4CG030500 Rh4CG030700 Rh4DG022100 Rh4DG022200 Rh4DG022300
rosa_wichuraiana	Rw4G002110 Rw4G002120 Rw4G002130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1755
AccB1I	GGYRCC	1 cut(s)	1794
AccII	CGCG	1 cut(s)	1025
AciI	CCGC	8 cut(s)	946, 1025, 1110, 1430, 1537, 1673, 2235, 2413
AclWI	GGATC	3 cut(s)	128, 1442, 2069
AcsI	RAATTY	5 cut(s)	935, 978, 1772, 1860, 2185
AcuI	CTGAAG	3 cut(s)	884, 932, 1731
AcvI	CACGTG	1 cut(s)	1127
AcyI	GRCGYC	2 cut(s)	1720, 1795
AfaI	GTAC	5 cut(s)	824, 955, 1666, 1875, 2154
AfiI	CCNNNNNNNGG	3 cut(s)	90, 1151, 1574
AflIII	ACRYGT	2 cut(s)	733, 1262
AgeI	ACCGGT	1 cut(s)	1158
AjnI	CCWGG	4 cut(s)	582, 1732, 1843, 2081
AjuI	GAANNNNNNNTTGG	6 cut(s)	256, 288, 369, 401, 723, 755
AluBI	AGCT	8 cut(s)	22, 30, 37, 513, 965, 1021, 1270, 2392
AluI	AGCT	8 cut(s)	22, 30, 37, 513, 965, 1021, 1270, 2392
Alw21I	GWGCWC	4 cut(s)	582, 844, 1272, 2407
Alw26I	GTCTC	2 cut(s)	70, 854
Alw44I	GTGCAC	1 cut(s)	840
AlwI	GGATC	3 cut(s)	128, 1442, 2069
Ama87I	CYCGRG	1 cut(s)	54
AoxI	GGCC	1 cut(s)	171
ApaLI	GTGCAC	1 cut(s)	840
ApeKI	GCWGC	5 cut(s)	22, 34, 413, 1077, 1112
ApoI	RAATTY	5 cut(s)	935, 978, 1772, 1860, 2185
AseI	ATTAAT	2 cut(s)	98, 267
AsiGI	ACCGGT	1 cut(s)	1158
Asp700I	GAANNNNTTC	2 cut(s)	2025, 2064
AspLEI	GCGC	2 cut(s)	1628, 1797
AspS9I	GGNCC	2 cut(s)	1763, 1841
AsuHPI	GGTGA	5 cut(s)	275, 1166, 1661, 1702, 2336
AsuII	TTCGAA	2 cut(s)	236, 1604
AvaI	CYCGRG	1 cut(s)	54
AvaII	GGWCC	2 cut(s)	1763, 1841
BaeGI	GKGCMC	1 cut(s)	844
BanI	GGYRCC	1 cut(s)	1794
BanII	GRGCYC	1 cut(s)	1272
BbrPI	CACGTG	1 cut(s)	1127
Bbv12I	GWGCWC	4 cut(s)	582, 844, 1272, 2407
BbvI	GCAGC	5 cut(s)	9, 46, 400, 1089, 1099
BccI	CCATC	6 cut(s)	295, 501, 1006, 1785, 1997, 2360
BceAI	ACGGC	1 cut(s)	2200
BcgI	CGANNNNNNTGC	2 cut(s)	216, 250
BciT130I	CCWGG	4 cut(s)	584, 1734, 1845, 2083
BclI	TGATCA	2 cut(s)	865, 1630
BcoDI	GTCTC	2 cut(s)	70, 854
BfaI	CTAG	3 cut(s)	966, 1427, 1649
BfmI	CTRYAG	4 cut(s)	960, 1305, 1369, 2552
BfoI	RGCGCY	2 cut(s)	1629, 1798
BglI	GCCNNNNNGGC	1 cut(s)	1121
BglII	AGATCT	2 cut(s)	1042, 1365
BisI	GCNGC	7 cut(s)	23, 35, 414, 1078, 1110, 1113, 2235
BlsI	GCNGC	7 cut(s)	24, 36, 415, 1079, 1111, 1114, 2236
Bme1390I	CCNGG	4 cut(s)	584, 1734, 1845, 2083
Bme18I	GGWCC	2 cut(s)	1763, 1841
BmeT110I	CYCGRG	1 cut(s)	54
BmgT120I	GGNCC	2 cut(s)	1763, 1841
BmiI	GGNNCC	3 cut(s)	60, 1764, 1796
BmrFI	CCNGG	4 cut(s)	584, 1734, 1845, 2083
BmsI	GCATC	2 cut(s)	1168, 1328
BoxI	GACNNNNGTC	1 cut(s)	293
BpmI	CTGGAG	1 cut(s)	878
Bpu10I	CCTNAGC	1 cut(s)	949
Bpu14I	TTCGAA	2 cut(s)	236, 1604
BpuEI	CTTGAG	3 cut(s)	569, 1724, 2513
BsaAI	YACGTR	2 cut(s)	1127, 1263
BsaBI	GATNNNNATC	1 cut(s)	1755
BsaHI	GRCGYC	2 cut(s)	1720, 1795
BsaJI	CCNNGG	2 cut(s)	2082, 2319
BsaWI	WCCGGW	1 cut(s)	1158
Bsc4I	CCNNNNNNNGG	3 cut(s)	90, 1151, 1574
Bse118I	RCCGGY	1 cut(s)	1158
Bse1I	ACTGG	4 cut(s)	174, 339, 1692, 2465
Bse3DI	GCAATG	2 cut(s)	1560, 2229
Bse8I	GATNNNNATC	1 cut(s)	1755
BseBI	CCWGG	4 cut(s)	584, 1734, 1845, 2083
BseDI	CCNNGG	2 cut(s)	2082, 2319
BseGI	GGATG	5 cut(s)	481, 540, 1648, 1796, 2048
BseJI	GATNNNNATC	1 cut(s)	1755
BseLI	CCNNNNNNNGG	3 cut(s)	90, 1151, 1574
BseMI	GCAATG	2 cut(s)	1560, 2229
BseMII	CTCAG	4 cut(s)	290, 844, 1299, 2265
BseNI	ACTGG	4 cut(s)	174, 339, 1692, 2465
BseSI	GKGCMC	1 cut(s)	844
BseXI	GCAGC	5 cut(s)	9, 46, 400, 1089, 1099
BsgI	GTGCAG	3 cut(s)	8, 276, 359
Bsh1236I	CGCG	1 cut(s)	1025
BshFI	GGCC	1 cut(s)	173
BshNI	GGYRCC	1 cut(s)	1794
BshTI	ACCGGT	1 cut(s)	1158
BsiHKAI	GWGCWC	4 cut(s)	582, 844, 1272, 2407
BsiHKCI	CYCGRG	1 cut(s)	54
BsiSI	CCGG	1 cut(s)	1159
BslFI	GGGAC	5 cut(s)	1214, 1368, 1707, 1776, 2203
BslI	CCNNNNNNNGG	3 cut(s)	90, 1151, 1574
BsmAI	GTCTC	2 cut(s)	70, 854
BsmBI	CGTCTC	1 cut(s)	70
BsmFI	GGGAC	5 cut(s)	1214, 1368, 1707, 1776, 2203
BsmI	GAATGC	1 cut(s)	1675
BsnI	GGCC	1 cut(s)	173
BsoBI	CYCGRG	1 cut(s)	54
Bsp119I	TTCGAA	2 cut(s)	236, 1604
Bsp1286I	GDGCHC	4 cut(s)	582, 844, 1272, 2407
Bsp1407I	TGTACA	1 cut(s)	822
BspACI	CCGC	8 cut(s)	946, 1025, 1110, 1430, 1537, 1673, 2235, 2413
BspANI	GGCC	1 cut(s)	173
BspCNI	CTCAG	4 cut(s)	289, 843, 1298, 2266
BspFNI	CGCG	1 cut(s)	1025
BspHI	TCATGA	2 cut(s)	931, 2202
BspLI	GGNNCC	3 cut(s)	60, 1764, 1796
BspPI	GGATC	3 cut(s)	128, 1442, 2069
BspQI	GCTCTTC	1 cut(s)	1206
BspT104I	TTCGAA	2 cut(s)	236, 1604
BspT107I	GGYRCC	1 cut(s)	1794
BsrDI	GCAATG	2 cut(s)	1560, 2229
BsrFI	RCCGGY	1 cut(s)	1158
BsrGI	TGTACA	1 cut(s)	822
BsrI	ACTGG	4 cut(s)	174, 339, 1692, 2465
BssAI	RCCGGY	1 cut(s)	1158
BssECI	CCNNGG	2 cut(s)	2082, 2319
BssNI	GRCGYC	2 cut(s)	1720, 1795
Bst2UI	CCWGG	4 cut(s)	584, 1734, 1845, 2083
Bst6I	CTCTTC	3 cut(s)	471, 1206, 2115
BstACI	GRCGYC	2 cut(s)	1720, 1795
BstAPI	GCANNNNNTGC	2 cut(s)	31, 1681
BstAUI	TGTACA	1 cut(s)	822
BstBAI	YACGTR	2 cut(s)	1127, 1263
BstBI	TTCGAA	2 cut(s)	236, 1604
BstC8I	GCNNGC	3 cut(s)	32, 592, 1023
BstDEI	CTNAG	7 cut(s)	276, 830, 949, 1082, 1285, 1740, 2274
BstEII	GGTNACC	1 cut(s)	1154
BstF5I	GGATG	5 cut(s)	481, 540, 1648, 1796, 2048
BstFNI	CGCG	1 cut(s)	1025
BstH2I	RGCGCY	2 cut(s)	1629, 1798
BstHHI	GCGC	2 cut(s)	1628, 1797
BstMAI	GTCTC	2 cut(s)	70, 854
BstMWI	GCNNNNNNNGC	5 cut(s)	31, 1121, 1681, 1792, 2270
BstNI	CCWGG	4 cut(s)	584, 1734, 1845, 2083
BstNSI	RCATGY	4 cut(s)	143, 147, 594, 687
BstPAI	GACNNNNGTC	1 cut(s)	293
BstPI	GGTNACC	1 cut(s)	1154
BstSCI	CCNGG	4 cut(s)	582, 1732, 1843, 2081
BstSFI	CTRYAG	4 cut(s)	960, 1305, 1369, 2552
BstSLI	GKGCMC	1 cut(s)	844
BstUI	CGCG	1 cut(s)	1025
BstV1I	GCAGC	5 cut(s)	9, 46, 400, 1089, 1099
BstX2I	RGATCY	3 cut(s)	1042, 1365, 2074
BstYI	RGATCY	3 cut(s)	1042, 1365, 2074
BsuRI	GGCC	1 cut(s)	173
BtsCI	GGATG	5 cut(s)	481, 540, 1648, 1796, 2048
BtsI	GCAGTG	1 cut(s)	1855
BtsIMutI	CAGTG	4 cut(s)	216, 1531, 1855, 2287
Cac8I	GCNNGC	3 cut(s)	32, 592, 1023
CciI	TCATGA	2 cut(s)	931, 2202
CfoI	GCGC	2 cut(s)	1628, 1797
Cfr10I	RCCGGY	1 cut(s)	1158
Cfr13I	GGNCC	2 cut(s)	1763, 1841
CseI	GACGC	1 cut(s)	1709
Csp6I	GTAC	5 cut(s)	823, 954, 1665, 1874, 2153
CspAI	ACCGGT	1 cut(s)	1158
CspCI	CAANNNNNGTGG	2 cut(s)	1222, 1257
CviAII	CATG	9 cut(s)	105, 140, 144, 591, 684, 932, 1219, 1457, 2203
CviQI	GTAC	5 cut(s)	823, 954, 1665, 1874, 2153
DdeI	CTNAG	7 cut(s)	276, 830, 949, 1082, 1285, 1740, 2274
DinI	GGCGCC	1 cut(s)	1796
Eam1104I	CTCTTC	3 cut(s)	471, 1206, 2115
EarI	CTCTTC	3 cut(s)	471, 1206, 2115
Ecl136II	GAGCTC	1 cut(s)	1270
Eco24I	GRGCYC	1 cut(s)	1272
Eco32I	GATATC	2 cut(s)	1060, 2347
Eco47I	GGWCC	2 cut(s)	1763, 1841
Eco53kI	GAGCTC	1 cut(s)	1270
Eco57I	CTGAAG	3 cut(s)	884, 932, 1731
Eco72I	CACGTG	1 cut(s)	1127
Eco88I	CYCGRG	1 cut(s)	54
Eco91I	GGTNACC	1 cut(s)	1154
EcoICRI	GAGCTC	1 cut(s)	1270
EcoO65I	GGTNACC	1 cut(s)	1154
EcoRII	CCWGG	4 cut(s)	582, 1732, 1843, 2081
EcoRV	GATATC	2 cut(s)	1060, 2347
EcoT22I	ATGCAT	1 cut(s)	145
EcoT38I	GRGCYC	1 cut(s)	1272
EgeI	GGCGCC	1 cut(s)	1796
EheI	GGCGCC	1 cut(s)	1796
Esp3I	CGTCTC	1 cut(s)	70
FaeI	CATG	9 cut(s)	108, 143, 147, 594, 687, 935, 1222, 1460, 2206
FalI	AAGNNNNNCTT	6 cut(s)	369, 401, 1493, 1525, 2295, 2327
FaqI	GGGAC	5 cut(s)	1214, 1368, 1707, 1776, 2203
FatI	CATG	9 cut(s)	104, 139, 143, 590, 683, 931, 1218, 1456, 2202
FauI	CCCGC	3 cut(s)	1423, 1530, 1680
FbaI	TGATCA	2 cut(s)	865, 1630
Fnu4HI	GCNGC	7 cut(s)	23, 35, 414, 1078, 1110, 1113, 2235
FokI	GGATG	5 cut(s)	488, 527, 1655, 1803, 2035
FriOI	GRGCYC	1 cut(s)	1272
Fsp4HI	GCNGC	7 cut(s)	23, 35, 414, 1078, 1110, 1113, 2235
FspBI	CTAG	3 cut(s)	966, 1427, 1649
GlaI	GCGC	2 cut(s)	1627, 1796
GluI	GCNGC	7 cut(s)	23, 35, 414, 1078, 1110, 1113, 2235
GsuI	CTGGAG	1 cut(s)	878
HaeII	RGCGCY	2 cut(s)	1629, 1798
HaeIII	GGCC	1 cut(s)	173
HapII	CCGG	1 cut(s)	1159
HgaI	GACGC	1 cut(s)	1709
HhaI	GCGC	2 cut(s)	1628, 1797
Hin1I	GRCGYC	2 cut(s)	1720, 1795
Hin1II	CATG	9 cut(s)	108, 143, 147, 594, 687, 935, 1222, 1460, 2206
Hin6I	GCGC	2 cut(s)	1626, 1795
HinP1I	GCGC	2 cut(s)	1626, 1795
HincII	GTYRAC	3 cut(s)	789, 1171, 2499
HindII	GTYRAC	3 cut(s)	789, 1171, 2499
HinfI	GANTC	8 cut(s)	203, 233, 650, 1223, 1356, 2158, 2180, 2504
HpaII	CCGG	1 cut(s)	1159
HphI	GGTGA	5 cut(s)	275, 1166, 1661, 1702, 2336
Hpy166II	GTNNAC	8 cut(s)	283, 789, 842, 889, 1171, 2179, 2285, 2499
Hpy188III	TCNNGA	9 cut(s)	298, 367, 497, 811, 857, 932, 1466, 1649, 2203
Hpy8I	GTNNAC	8 cut(s)	283, 789, 842, 889, 1171, 2179, 2285, 2499
HpyAV	CCTTC	6 cut(s)	60, 498, 1144, 2388, 2446, 2525
HpyCH4IV	ACGT	4 cut(s)	566, 733, 1126, 1262
HpyF10VI	GCNNNNNNNGC	5 cut(s)	31, 1121, 1681, 1792, 2270
HpyF3I	CTNAG	7 cut(s)	276, 830, 949, 1082, 1285, 1740, 2274
HpySE526I	ACGT	4 cut(s)	566, 733, 1126, 1262
Hsp92I	GRCGYC	2 cut(s)	1720, 1795
Hsp92II	CATG	9 cut(s)	108, 143, 147, 594, 687, 935, 1222, 1460, 2206
HspAI	GCGC	2 cut(s)	1626, 1795
KasI	GGCGCC	1 cut(s)	1794
Ksp22I	TGATCA	2 cut(s)	865, 1630
LguI	GCTCTTC	1 cut(s)	1206
LmnI	GCTCC	2 cut(s)	510, 585
Lsp1109I	GCAGC	5 cut(s)	9, 46, 400, 1089, 1099
LweI	GCATC	2 cut(s)	1168, 1328
MaeI	CTAG	3 cut(s)	966, 1427, 1649
MaeII	ACGT	4 cut(s)	566, 733, 1126, 1262
MaeIII	GTNAC	8 cut(s)	107, 295, 522, 1154, 1449, 1594, 2044, 2257
MfeI	CAATTG	1 cut(s)	1236
MflI	RGATCY	3 cut(s)	1042, 1365, 2074
MhlI	GDGCHC	4 cut(s)	582, 844, 1272, 2407
Mly113I	GGCGCC	1 cut(s)	1795
MlyI	GAGTC	3 cut(s)	659, 1350, 2174
MmeI	TCCRAC	4 cut(s)	93, 453, 568, 2102
Mph1103I	ATGCAT	1 cut(s)	145
MroXI	GAANNNNTTC	2 cut(s)	2025, 2064
MslI	CAYNNNNRTG	2 cut(s)	688, 1518
MspA1I	CMGCKG	2 cut(s)	22, 1112
MspI	CCGG	1 cut(s)	1159
MspR9I	CCNGG	4 cut(s)	584, 1734, 1845, 2083
MunI	CAATTG	1 cut(s)	1236
Mva1269I	GAATGC	1 cut(s)	1675
MvaI	CCWGG	4 cut(s)	584, 1734, 1845, 2083
MvnI	CGCG	1 cut(s)	1025
MwoI	GCNNNNNNNGC	5 cut(s)	31, 1121, 1681, 1792, 2270
NarI	GGCGCC	1 cut(s)	1795
NlaIII	CATG	9 cut(s)	108, 143, 147, 594, 687, 935, 1222, 1460, 2206
NlaIV	GGNNCC	3 cut(s)	60, 1764, 1796
NmeAIII	GCCGAG	1 cut(s)	2344
NmuCI	GTSAC	3 cut(s)	107, 295, 1154
NsiI	ATGCAT	1 cut(s)	145
NspI	RCATGY	4 cut(s)	143, 147, 594, 687
NspV	TTCGAA	2 cut(s)	236, 1604
PaeI	GCATGC	1 cut(s)	594
PagI	TCATGA	2 cut(s)	931, 2202
PciSI	GCTCTTC	1 cut(s)	1206
PcsI	WCGNNNNNNNCGW	1 cut(s)	730
PctI	GAATGC	1 cut(s)	1675
PdmI	GAANNNNTTC	2 cut(s)	2025, 2064
PfeI	GAWTC	5 cut(s)	203, 233, 1223, 2158, 2504
PinAI	ACCGGT	1 cut(s)	1158
PkrI	GCNGC	7 cut(s)	24, 36, 415, 1079, 1111, 1114, 2236
PleI	GAGTC	3 cut(s)	658, 1350, 2174
PluTI	GGCGCC	1 cut(s)	1798
PmaCI	CACGTG	1 cut(s)	1127
PmlI	CACGTG	1 cut(s)	1127
PpsI	GAGTC	3 cut(s)	658, 1350, 2174
Ppu21I	YACGTR	2 cut(s)	1127, 1263
PshAI	GACNNNNGTC	1 cut(s)	293
PshBI	ATTAAT	2 cut(s)	98, 267
PsiI	TTATAA	1 cut(s)	1755
Psp124BI	GAGCTC	1 cut(s)	1272
Psp6I	CCWGG	4 cut(s)	582, 1732, 1843, 2081
PspCI	CACGTG	1 cut(s)	1127
PspEI	GGTNACC	1 cut(s)	1154
PspGI	CCWGG	4 cut(s)	582, 1732, 1843, 2081
PspN4I	GGNNCC	3 cut(s)	60, 1764, 1796
PspPI	GGNCC	2 cut(s)	1763, 1841
PsrI	GAACNNNNNNTAC	2 cut(s)	806, 838
PsuI	RGATCY	3 cut(s)	1042, 1365, 2074
PvuII	CAGCTG	1 cut(s)	22
RsaI	GTAC	5 cut(s)	824, 955, 1666, 1875, 2154
RsaNI	GTAC	5 cut(s)	823, 954, 1665, 1874, 2153
RseI	CAYNNNNRTG	2 cut(s)	688, 1518
SacI	GAGCTC	1 cut(s)	1272
SapI	GCTCTTC	1 cut(s)	1206
SatI	GCNGC	7 cut(s)	23, 35, 414, 1078, 1110, 1113, 2235
Sau96I	GGNCC	2 cut(s)	1763, 1841
SchI	GAGTC	3 cut(s)	659, 1350, 2174
ScrFI	CCNGG	4 cut(s)	584, 1734, 1845, 2083
SduI	GDGCHC	4 cut(s)	582, 844, 1272, 2407
SfaNI	GCATC	2 cut(s)	1168, 1328
SfcI	CTRYAG	4 cut(s)	960, 1305, 1369, 2552
SfoI	GGCGCC	1 cut(s)	1796
SfuI	TTCGAA	2 cut(s)	236, 1604
SinI	GGWCC	2 cut(s)	1763, 1841
SmiMI	CAYNNNNRTG	2 cut(s)	688, 1518
SmlI	CTYRAG	3 cut(s)	548, 1703, 2492
SmoI	CTYRAG	3 cut(s)	548, 1703, 2492
SphI	GCATGC	1 cut(s)	594
SsiI	CCGC	8 cut(s)	946, 1025, 1110, 1430, 1537, 1673, 2235, 2413
SspDI	GGCGCC	1 cut(s)	1794
SspI	AATATT	2 cut(s)	271, 2128
SspMI	CTAG	3 cut(s)	966, 1427, 1649
SstI	GAGCTC	1 cut(s)	1272
StyD4I	CCNGG	4 cut(s)	582, 1732, 1843, 2081
TaiI	ACGT	4 cut(s)	569, 736, 1129, 1265
TaqI	TCGA	5 cut(s)	236, 1280, 1604, 2183, 2529
TatI	WGTACW	1 cut(s)	822
TauI	GCSGC	2 cut(s)	1112, 2237
TfiI	GAWTC	5 cut(s)	203, 233, 1223, 2158, 2504
TscAI	CASTG	4 cut(s)	216, 1531, 1855, 2287
TseFI	GTSAC	3 cut(s)	107, 295, 1154
TseI	GCWGC	5 cut(s)	22, 34, 413, 1077, 1112
Tsp45I	GTSAC	3 cut(s)	107, 295, 1154
TspGWI	ACGGA	2 cut(s)	120, 1932
TspRI	CASTG	4 cut(s)	216, 1531, 1855, 2287
VneI	GTGCAC	1 cut(s)	840
VpaK11BI	GGWCC	2 cut(s)	1763, 1841
VspI	ATTAAT	2 cut(s)	98, 267
XapI	RAATTY	5 cut(s)	935, 978, 1772, 1860, 2185
XbaI	TCTAGA	1 cut(s)	1648
XceI	RCATGY	4 cut(s)	143, 147, 594, 687
XcmI	CCANNNNNNNNNTGG	2 cut(s)	111, 571
XmnI	GAANNNNTTC	2 cut(s)	2025, 2064
XspI	CTAG	3 cut(s)	966, 1427, 1649
Zsp2I	ATGCAT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.