FvH4_4g17150

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000853
Physical Location & Seq
Forward (+)
21043686 .. 21045407
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17150.t1

Sequence Viewer

Length: 1509 bp
ATGGAGACATGGTTGCTCATCCTCAGCTCCATCTTTATCTCTATGTTCCTCATGTTAATTCTCTCTGTCCATCCCAAGACGAAGCAGCTTCCGCCAGGACCCTTTACCATCCCCATAATCAGCGGCTTTTTATGGCTCCGCAAACCCTTCACGGAGCTTGAGCCAGCCATCCGGAATATCCAGGCCCACTACGGCCCAATTATCACCGTCCAAATTGGCTCCCAGCCCTCCATCTTCATCTCCAGCCGCTCCTTGGCCCACCAGGCCCTGATCCAAAACGGCGCTGTCTTCGCCGACCGCCCACCAGCCTCGGCCACCACCAAAATATTCAGCAGCAACCAACACGAAATCAACTCTGCCCCTTACGGCCCCACGTGGCGCCTTCTTCGCCGCAACCTCACCTCAGAAATGCTCAACCATTCTCGCTTGAAATCCTACAACGGGGCTCGCAAATGGGTCCTCAACATCCTCGCCAACCGCCTAATGAAATCTCAATCCAAATCGAACGGAGTCAAAGTCATAGACCATTTCCAATACGCCATGTTCTCGATGCTTGTACTTATGTGTTTTGGTGATAAACTTGATGAGAAGCAAATAAATCAAATACGACATCTCCAAAGACCGCAACTGCTGACTAATGGCCGGTTCAACATTCTCAATTTTTGCCCTTCAATCACGAAAATTGTATTGAAGAAGTGGTGGGCGGAGTTCCTCCAGTACCGAAAAGACCAAGAAGATGTCCTAGTCCCTCTAATTAGAGCCCGGAAGGAGGCCAAGGTAAAAGAGGAGGGTGAAAATTATGTGCTATCTTACGTCGACACGCTCTGTGGTCTTGAGCTTCCTGACAAGAAGAGGAAGCTGACTGAAGATGAAATCATAAGCCTATGTTCAGAATTTATGAATGCAGGTGTAGATACTACTACAACTGCATTACAGTGGATCATAGCAAACATTGTCAAGTATCCAACGGTTCAAGAGCAGCTTTTTGTAGAGATTAAAGGAGTTGTGGGGGAAACAGAGGAGGTGAGAGAGGAGGTCTTGCAGAAGATGCCTTATCTACAAGCAGTAATCTTGGAGGGGCTGAGGCGCCACCCGCCGGCCCATCTTTTGAGTCCGCATTCTGTCACACAAGATATCATTTTGGGAGGTCATGTGGTGCCTAAGAAAGGCACGGTCAATTTTTTGGTGGCGGACATGGGGTGGGACCCAGAGGTGTGGGAGAACCCTATGGATTTTAAGCCGGAGAGGTTTTTGAATAACGGAAATTATGAGTTTGATATAACTGGGAGCAAAGAGATTAAGATGATGCCGTTTGGGGCTGGGAGAAGAATTTGCCCTGGCCTTGGTTTAGCGTTGCTTAATCTCAAGTATTTTGTGGCGAATTTGGTCTGGAAATTCGAGTGGAGAGGTTTAGAAGGAGAGCATGTTGACTTGACTGAAAAGCAAGAGTTTACCATGGTCATGAAGAATCCATTGAAGGCTCACATTTCACCAAGAGTCGTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

503

Amino Acids

57.44

Weight (kDa)

9.17

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 493 1e-93 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 896
Acc36I ACCTGC 1 cut(s) 896
AccB1I GGYRCC 3 cut(s) 378, 1086, 1156
AccBSI CCGCTC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 816
AccIII TCCGGA 1 cut(s) 171
AclWI GGATC 2 cut(s) 265, 947
AcoI YGGCCR 2 cut(s) 312, 640
AcsI RAATTY 4 cut(s) 893, 1329, 1381, 1394
AcuI CTGAAG 1 cut(s) 885
AcvI CACGTG 1 cut(s) 375
AcyI GRCGYC 2 cut(s) 379, 1087
AfaI GTAC 2 cut(s) 558, 719
AfiI CCNNNNNNNGG 6 cut(s) 253, 441, 769, 1096, 1166, 1343
AgsI TTSAA 7 cut(s) 430, 649, 672, 691, 974, 1255, 1477
AjnI CCWGG 4 cut(s) 94, 180, 261, 1336
AluBI AGCT 6 cut(s) 27, 88, 157, 838, 859, 982
AluI AGCT 6 cut(s) 27, 88, 157, 838, 859, 982
AlwI GGATC 2 cut(s) 265, 947
AlwNI CAGNNNCTG 1 cut(s) 268
Aor13HI TCCGGA 1 cut(s) 171
ApeKI GCWGC 3 cut(s) 85, 333, 979
ApoI RAATTY 4 cut(s) 893, 1329, 1381, 1394
AspLEI GCGC 3 cut(s) 284, 381, 1089
AspS9I GGNCC 9 cut(s) 98, 184, 194, 256, 265, 368, 457, 1099, 1204
AsuC2I CCSGG 1 cut(s) 763
AsuHPI GGTGA 6 cut(s) 196, 391, 584, 803, 1036, 1482
AvaII GGWCC 3 cut(s) 98, 457, 1204
BanI GGYRCC 3 cut(s) 378, 1086, 1156
BanII GRGCYC 2 cut(s) 448, 763
BbrPI CACGTG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 280
BbvCI CCTCAGC 2 cut(s) 23, 1082
BbvI GCAGC 3 cut(s) 97, 345, 991
BccI CCATC 6 cut(s) 38, 78, 116, 176, 239, 1110
BceAI ACGGC 4 cut(s) 208, 295, 382, 1294
BciT130I CCWGG 4 cut(s) 96, 182, 263, 1338
BciVI GTATCC 1 cut(s) 972
BcnI CCSGG 1 cut(s) 763
BfaI CTAG 1 cut(s) 743
BfoI RGCGCY 3 cut(s) 285, 382, 1090
BfuAI ACCTGC 1 cut(s) 896
BfuI GTATCC 1 cut(s) 972
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 6 cut(s) 86, 124, 247, 334, 391, 980
BlsI GCNGC 6 cut(s) 87, 125, 248, 335, 392, 981
Bme1390I CCNGG 5 cut(s) 96, 182, 263, 763, 1338
Bme18I GGWCC 3 cut(s) 98, 457, 1204
BmgT120I GGNCC 9 cut(s) 98, 184, 194, 256, 265, 368, 457, 1099, 1204
BmrFI CCNGG 5 cut(s) 96, 182, 263, 763, 1338
BmrI ACTGGG 1 cut(s) 1293
BmsI GCATC 3 cut(s) 540, 1038, 1296
BmuI ACTGGG 1 cut(s) 1293
BpiI GAAGAC 1 cut(s) 280
BpmI CTGGAG 2 cut(s) 226, 698
Bpu10I CCTNAGC 2 cut(s) 23, 1082
BpuEI CTTGAG 3 cut(s) 179, 854, 1349
BpuMI CCSGG 1 cut(s) 763
BsaAI YACGTR 1 cut(s) 375
BsaHI GRCGYC 2 cut(s) 379, 1087
BsaJI CCNNGG 6 cut(s) 252, 309, 774, 1336, 1342, 1455
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 9 cut(s) 26, 501, 531, 597, 627, 1137, 1167, 1410, 1440
Bsc4I CCNNNNNNNGG 6 cut(s) 253, 441, 769, 1096, 1166, 1343
Bse118I RCCGGY 2 cut(s) 642, 1096
Bse1I ACTGG 2 cut(s) 715, 1288
BseAI TCCGGA 1 cut(s) 171
BseBI CCWGG 4 cut(s) 96, 182, 263, 1338
BseDI CCNNGG 6 cut(s) 252, 309, 774, 1336, 1342, 1455
BseGI GGATG 5 cut(s) 18, 70, 108, 168, 465
BseLI CCNNNNNNNGG 6 cut(s) 253, 441, 769, 1096, 1166, 1343
BseMII CTCAG 3 cut(s) 37, 417, 1073
BseNI ACTGG 2 cut(s) 715, 1288
BseRI GAGGAG 3 cut(s) 800, 1034, 1046
BseXI GCAGC 3 cut(s) 97, 345, 991
BseYI CCCAGC 2 cut(s) 222, 1319
Bsh1285I CGRYCG 1 cut(s) 298
BshNI GGYRCC 3 cut(s) 378, 1086, 1156
BsiEI CGRYCG 1 cut(s) 298
BsiSI CCGG 5 cut(s) 172, 643, 763, 1097, 1241
BslFI GGGAC 2 cut(s) 731, 1217
BslI CCNNNNNNNGG 6 cut(s) 253, 441, 769, 1096, 1166, 1343
BsmFI GGGAC 2 cut(s) 731, 1217
BsmI GAATGC 2 cut(s) 907, 1117
Bsp1286I GDGCHC 2 cut(s) 448, 763
Bsp13I TCCGGA 1 cut(s) 171
Bsp143I GATC 2 cut(s) 270, 939
Bsp19I CCATGG 1 cut(s) 1455
BspCNI CTCAG 3 cut(s) 36, 416, 1074
BspEI TCCGGA 1 cut(s) 171
BspHI TCATGA 1 cut(s) 1461
BspMI ACCTGC 1 cut(s) 896
BspPI GGATC 2 cut(s) 265, 947
BspT107I GGYRCC 3 cut(s) 378, 1086, 1156
BsrBI CCGCTC 1 cut(s) 249
BsrFI RCCGGY 2 cut(s) 642, 1096
BsrI ACTGG 2 cut(s) 715, 1288
BssAI RCCGGY 2 cut(s) 642, 1096
BssECI CCNNGG 6 cut(s) 252, 309, 774, 1336, 1342, 1455
BssMI GATC 2 cut(s) 270, 939
BssNI GRCGYC 2 cut(s) 379, 1087
BssT1I CCWWGG 4 cut(s) 252, 774, 1342, 1455
Bst2UI CCWGG 4 cut(s) 96, 182, 263, 1338
Bst4CI ACNGT 4 cut(s) 208, 936, 970, 1174
Bst6I CTCTTC 1 cut(s) 845
BstACI GRCGYC 2 cut(s) 379, 1087
BstAPI GCANNNNNTGC 1 cut(s) 1048
BstBAI YACGTR 1 cut(s) 375
BstC8I GCNNGC 3 cut(s) 165, 448, 1098
BstDEI CTNAG 4 cut(s) 23, 403, 1082, 1161
BstDSI CCRYGG 1 cut(s) 1455
BstENI CCTNNNNNAGG 1 cut(s) 1164
BstF5I GGATG 5 cut(s) 18, 70, 108, 168, 465
BstH2I RGCGCY 3 cut(s) 285, 382, 1090
BstHHI GCGC 3 cut(s) 284, 381, 1089
BstKTI GATC 2 cut(s) 273, 942
BstMBI GATC 2 cut(s) 270, 939
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 6 cut(s) 91, 263, 290, 387, 1048, 1093
BstNI CCWGG 4 cut(s) 96, 182, 263, 1338
BstNSI RCATGY 1 cut(s) 1427
BstSCI CCNGG 5 cut(s) 94, 180, 261, 761, 1336
BstV1I GCAGC 3 cut(s) 97, 345, 991
BstV2I GAAGAC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 1215
BsuI GTATCC 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 1455
BtsCI GGATG 5 cut(s) 18, 70, 108, 168, 465
BtsIMutI CAGTG 1 cut(s) 941
BveI ACCTGC 1 cut(s) 896
Cac8I GCNNGC 3 cut(s) 165, 448, 1098
CaiI CAGNNNCTG 1 cut(s) 268
CciI TCATGA 1 cut(s) 1461
CfoI GCGC 3 cut(s) 284, 381, 1089
Cfr10I RCCGGY 2 cut(s) 642, 1096
Cfr13I GGNCC 9 cut(s) 98, 184, 194, 256, 265, 368, 457, 1099, 1204
Csp6I GTAC 2 cut(s) 557, 718
CviAII CATG 8 cut(s) 9, 52, 541, 1151, 1195, 1424, 1456, 1462
CviQI GTAC 2 cut(s) 557, 718
DdeI CTNAG 4 cut(s) 23, 403, 1082, 1161
DinI GGCGCC 2 cut(s) 380, 1088
DpnI GATC 2 cut(s) 272, 941
DpnII GATC 2 cut(s) 270, 939
EaeI YGGCCR 2 cut(s) 312, 640
Eam1104I CTCTTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 845
EciI GGCGGA 3 cut(s) 81, 719, 1205
Eco130I CCWWGG 4 cut(s) 252, 774, 1342, 1455
Eco24I GRGCYC 2 cut(s) 448, 763
Eco32I GATATC 1 cut(s) 1135
Eco47I GGWCC 3 cut(s) 98, 457, 1204
Eco57I CTGAAG 1 cut(s) 885
Eco72I CACGTG 1 cut(s) 375
EcoNI CCTNNNNNAGG 1 cut(s) 1164
EcoO109I RGGNCCY 4 cut(s) 98, 265, 457, 1204
EcoRII CCWGG 4 cut(s) 94, 180, 261, 1336
EcoRV GATATC 1 cut(s) 1135
EcoT14I CCWWGG 4 cut(s) 252, 774, 1342, 1455
EcoT38I GRGCYC 2 cut(s) 448, 763
EgeI GGCGCC 2 cut(s) 380, 1088
EheI GGCGCC 2 cut(s) 380, 1088
ErhI CCWWGG 4 cut(s) 252, 774, 1342, 1455
FaeI CATG 8 cut(s) 12, 55, 544, 1154, 1198, 1427, 1459, 1465
FalI AAGNNNNNCTT 2 cut(s) 966, 998
FaqI GGGAC 2 cut(s) 731, 1217
FatI CATG 8 cut(s) 8, 51, 540, 1150, 1194, 1423, 1455, 1461
FauI CCCGC 1 cut(s) 1101
FblI GTMKAC 1 cut(s) 816
Fnu4HI GCNGC 6 cut(s) 86, 124, 247, 334, 391, 980
FokI GGATG 5 cut(s) 5, 57, 95, 155, 452
FriOI GRGCYC 2 cut(s) 448, 763
Fsp4HI GCNGC 6 cut(s) 86, 124, 247, 334, 391, 980
FspBI CTAG 1 cut(s) 743
GlaI GCGC 3 cut(s) 283, 380, 1088
GluI GCNGC 6 cut(s) 86, 124, 247, 334, 391, 980
GsaI CCCAGC 2 cut(s) 226, 1323
GsuI CTGGAG 2 cut(s) 226, 698
HaeII RGCGCY 3 cut(s) 285, 382, 1090
HapII CCGG 5 cut(s) 172, 643, 763, 1097, 1241
HhaI GCGC 3 cut(s) 284, 381, 1089
Hin1I GRCGYC 2 cut(s) 379, 1087
Hin1II CATG 8 cut(s) 12, 55, 544, 1154, 1198, 1427, 1459, 1465
Hin6I GCGC 3 cut(s) 282, 379, 1087
HinP1I GCGC 3 cut(s) 282, 379, 1087
HincII GTYRAC 2 cut(s) 817, 1429
HindII GTYRAC 2 cut(s) 817, 1429
HinfI GANTC 4 cut(s) 510, 1111, 1468, 1497
HpaII CCGG 5 cut(s) 172, 643, 763, 1097, 1241
HphI GGTGA 6 cut(s) 196, 391, 584, 803, 1036, 1482
Hpy166II GTNNAC 3 cut(s) 817, 1429, 1452
Hpy188I TCNGA 2 cut(s) 406, 892
Hpy188III TCNNGA 9 cut(s) 172, 547, 676, 833, 842, 974, 1390, 1462, 1501
Hpy8I GTNNAC 3 cut(s) 817, 1429, 1452
Hpy99I CGWCG 1 cut(s) 818
HpyAV CCTTC 6 cut(s) 157, 392, 678, 760, 1409, 1471
HpyCH4III ACNGT 4 cut(s) 208, 936, 970, 1174
HpyCH4IV ACGT 2 cut(s) 374, 813
HpyCH4V TGCA 3 cut(s) 905, 929, 1042
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 263, 290, 387, 1048, 1093
HpyF3I CTNAG 4 cut(s) 23, 403, 1082, 1161
HpySE526I ACGT 2 cut(s) 374, 813
Hsp92I GRCGYC 2 cut(s) 379, 1087
Hsp92II CATG 8 cut(s) 12, 55, 544, 1154, 1198, 1427, 1459, 1465
HspAI GCGC 3 cut(s) 282, 379, 1087
KasI GGCGCC 2 cut(s) 378, 1086
KflI GGGWCCC 1 cut(s) 1204
Kpn2I TCCGGA 1 cut(s) 171
KroI GCCGGC 1 cut(s) 1096
KroNI GCCGGC 1 cut(s) 1098
Kzo9I GATC 2 cut(s) 270, 939
LmnI GCTCC 6 cut(s) 32, 141, 154, 224, 254, 1287
Lsp1109I GCAGC 3 cut(s) 97, 345, 991
LweI GCATC 3 cut(s) 540, 1038, 1296
MaeI CTAG 1 cut(s) 743
MaeII ACGT 2 cut(s) 374, 813
MaeIII GTNAC 1 cut(s) 1123
MalI GATC 2 cut(s) 272, 941
MbiI CCGCTC 1 cut(s) 249
MboI GATC 2 cut(s) 270, 939
MhlI GDGCHC 2 cut(s) 448, 763
Mly113I GGCGCC 2 cut(s) 379, 1087
MlyI GAGTC 3 cut(s) 519, 1120, 1506
MmeI TCCRAC 1 cut(s) 989
MroI TCCGGA 1 cut(s) 171
MroNI GCCGGC 1 cut(s) 1096
MseI TTAA 5 cut(s) 56, 996, 1236, 1299, 1359
MslI CAYNNNNRTG 2 cut(s) 934, 1460
MspA1I CMGCKG 1 cut(s) 123
MspI CCGG 5 cut(s) 172, 643, 763, 1097, 1241
MspR9I CCNGG 5 cut(s) 96, 182, 263, 763, 1338
Mva1269I GAATGC 2 cut(s) 907, 1117
MvaI CCWGG 4 cut(s) 96, 182, 263, 1338
MwoI GCNNNNNNNGC 6 cut(s) 91, 263, 290, 387, 1048, 1093
NaeI GCCGGC 1 cut(s) 1098
NarI GGCGCC 2 cut(s) 379, 1087
NciI CCSGG 1 cut(s) 763
NcoI CCATGG 1 cut(s) 1455
NdeII GATC 2 cut(s) 270, 939
NgoMIV GCCGGC 1 cut(s) 1096
NlaIII CATG 8 cut(s) 12, 55, 544, 1154, 1198, 1427, 1459, 1465
NmeAIII GCCGAG 1 cut(s) 290
NmuCI GTSAC 1 cut(s) 1123
NspI RCATGY 1 cut(s) 1427
PagI TCATGA 1 cut(s) 1461
PaqCI CACCTGC 1 cut(s) 896
PctI GAATGC 2 cut(s) 907, 1117
PdiI GCCGGC 1 cut(s) 1098
PfeI GAWTC 1 cut(s) 1468
PkrI GCNGC 6 cut(s) 87, 125, 248, 335, 392, 981
PleI GAGTC 3 cut(s) 518, 1119, 1505
PluTI GGCGCC 2 cut(s) 382, 1090
PmaCI CACGTG 1 cut(s) 375
PmlI CACGTG 1 cut(s) 375
PpsI GAGTC 3 cut(s) 518, 1119, 1505
Ppu21I YACGTR 1 cut(s) 375
PpuMI RGGWCCY 3 cut(s) 98, 457, 1204
Psp5II RGGWCCY 3 cut(s) 98, 457, 1204
Psp6I CCWGG 4 cut(s) 94, 180, 261, 1336
PspCI CACGTG 1 cut(s) 375
PspFI CCCAGC 2 cut(s) 222, 1319
PspGI CCWGG 4 cut(s) 94, 180, 261, 1336
PspPI GGNCC 9 cut(s) 98, 184, 194, 256, 265, 368, 457, 1099, 1204
PspPPI RGGWCCY 3 cut(s) 98, 457, 1204
PstNI CAGNNNCTG 1 cut(s) 268
RsaI GTAC 2 cut(s) 558, 719
RsaNI GTAC 2 cut(s) 557, 718
RseI CAYNNNNRTG 2 cut(s) 934, 1460
SalI GTCGAC 1 cut(s) 815
SaqAI TTAA 5 cut(s) 56, 996, 1236, 1299, 1359
SatI GCNGC 6 cut(s) 86, 124, 247, 334, 391, 980
Sau3AI GATC 2 cut(s) 270, 939
Sau96I GGNCC 9 cut(s) 98, 184, 194, 256, 265, 368, 457, 1099, 1204
SchI GAGTC 3 cut(s) 519, 1120, 1506
ScrFI CCNGG 5 cut(s) 96, 182, 263, 763, 1338
SduI GDGCHC 2 cut(s) 448, 763
SfaNI GCATC 3 cut(s) 540, 1038, 1296
SfiI GGCCNNNNNGGCC 1 cut(s) 263
SfoI GGCGCC 2 cut(s) 380, 1088
SinI GGWCC 3 cut(s) 98, 457, 1204
SmiMI CAYNNNNRTG 2 cut(s) 934, 1460
SmlI CTYRAG 3 cut(s) 158, 833, 1364
SmoI CTYRAG 3 cut(s) 158, 833, 1364
SspDI GGCGCC 2 cut(s) 378, 1086
SspI AATATT 1 cut(s) 327
SspMI CTAG 1 cut(s) 743
StyD4I CCNGG 5 cut(s) 94, 180, 261, 761, 1336
StyI CCWWGG 4 cut(s) 252, 774, 1342, 1455
TaaI ACNGT 4 cut(s) 208, 936, 970, 1174
TaiI ACGT 2 cut(s) 377, 816
TaqI TCGA 4 cut(s) 503, 548, 816, 1398
TatI WGTACW 1 cut(s) 556
TauI GCSGC 3 cut(s) 126, 249, 393
TfiI GAWTC 1 cut(s) 1468
Tru1I TTAA 5 cut(s) 56, 996, 1236, 1299, 1359
Tru9I TTAA 5 cut(s) 56, 996, 1236, 1299, 1359
TscAI CASTG 1 cut(s) 941
TseFI GTSAC 1 cut(s) 1123
TseI GCWGC 3 cut(s) 85, 333, 979
Tsp45I GTSAC 1 cut(s) 1123
TspDTI ATGAA 5 cut(s) 226, 500, 885, 914, 1478
TspGWI ACGGA 3 cut(s) 167, 522, 1275
TspRI CASTG 1 cut(s) 941
VpaK11BI GGWCC 3 cut(s) 98, 457, 1204
XagI CCTNNNNNAGG 1 cut(s) 1164
XapI RAATTY 4 cut(s) 893, 1329, 1381, 1394
XceI RCATGY 1 cut(s) 1427
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 816
XspI CTAG 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.