Rw1G004050

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000853
Physical Location & Seq
Reverse (-)
7592677 .. 7593833
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004050.1

Sequence Viewer

Length: 1059 bp
ATGCTGTTAATTTTCCTGAGAGTGACTATCAATTTGCTATCACCAAGACAACACTTTTTTCGAATACGCTGGTGGCATTGCCAACCGGACTCGGAATCCCAATCCCAATCCCAATCCCAATCCAAATCCAAATCCAACGGAGGGGGAGTCAAAGTCGTTGACCATTTCCAGTATGCCATGTTCTGCTTGCTGATTCTCATTAAAATCAAAGAGGTCCAGCGTGGGCTAATCCTCAGCCTCGGCCAGTTCAACATCCTCAATTCCTGGCCTTCATTGACGAAAATCCTCCTCAAGAATCCCTGGCAGGAGTTTTTTCGAATCCGAAAAGCACAAGAAGATGTCCTTCTTCCTCTGATTCGAGCCCAAAAGGATGCCATGGTAAACAAGAGCGATGATTATGTACTATGTTACCTAGACACGCTCTGGGAACTCAAGCTTCCGGACGAGAAGAGGAAGCTGAACAAAAAAGATATCATTAGCCTCTATTTGGAGTTTCTCAGTGCAGGAACGGATACTACTTCCACCGCGCTGCAGTGGATCATGGCCAATATCGTCAAGTATCCAATTATCCAGGAGAGGCTTCTCGCTGAGATTAAAGGAGTTGTGGAGGAAACAGAGGAGGTGGTGAAGGAGGAGGACTTGCAGAAGATGCCTTATTTAAAAGCGGTGATCTTGGAGGGTTTAAGGCGCCACCCACCTGGCCACTTTGCGCTGCCGCATTCCATCACGCACGACATCGTTTTGGATGGTTATGTGGTGTGGGAAGAGCCTATGGAGTTTAAGCCGGAGTGGTTTTTGAGTAAGGAGGGAGGAGATCAGGGGTTTGATATAATGGGGAGCAGAGAGATTAAGATGATGCCGTTTGGGGTTGGGAGGAAGATTTGCCCTGGACTTGGTTTGGCTGTGCTTCATCTGGAGTTTTTTGTGGCGAATTTGGTGTGGAAATTCGAGTGGAGAGGTGTGGAGGGAGAGGATGTTGACTTCGCGGAGAAGCAAGAGTTCACAATGGTTATGAGGAATCCATTGAAGGCGAACATTTCACCAAGAGTGATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

352

Amino Acids

40.86

Weight (kDa)

7.1

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 80 - 253 7.3e-36 Cytochrome P450
p450 PF00067 253 - 344 1.2e-14 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 687
AccII CGCG 2 cut(s) 527, 986
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 4 cut(s) 525, 665, 716, 986
AclWI GGATC 1 cut(s) 545
AcoI YGGCCR 3 cut(s) 241, 543, 700
AcsI RAATTY 2 cut(s) 931, 944
AcyI GRCGYC 1 cut(s) 688
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 3 cut(s) 141, 487, 893
AgsI TTSAA 2 cut(s) 250, 1027
AjnI CCWGG 5 cut(s) 263, 299, 570, 697, 886
AluBI AGCT 2 cut(s) 436, 457
AluI AGCT 2 cut(s) 436, 457
AlwI GGATC 1 cut(s) 545
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 4 cut(s) 241, 266, 543, 700
ApeKI GCWGC 2 cut(s) 529, 712
ApoI RAATTY 2 cut(s) 931, 944
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AspLEI GCGC 3 cut(s) 529, 690, 712
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 4 cut(s) 33, 637, 679, 1032
AsuII TTCGAA 2 cut(s) 61, 316
AvaII GGWCC 1 cut(s) 214
BalI TGGCCA 2 cut(s) 545, 702
BanI GGYRCC 1 cut(s) 687
BanII GRGCYC 1 cut(s) 364
BbvCI CCTCAGC 1 cut(s) 233
BbvI GCAGC 2 cut(s) 516, 699
BccI CCATC 2 cut(s) 731, 740
BceAI ACGGC 1 cut(s) 844
BciT130I CCWGG 5 cut(s) 265, 301, 572, 699, 888
BciVI GTATCC 2 cut(s) 505, 570
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 691
BfuI GTATCC 2 cut(s) 505, 570
BisI GCNGC 3 cut(s) 530, 713, 716
BlsI GCNGC 3 cut(s) 531, 714, 717
Bme1390I CCNGG 5 cut(s) 265, 301, 572, 699, 888
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 689
BmrFI CCNGG 5 cut(s) 265, 301, 572, 699, 888
BmsI GCATC 3 cut(s) 361, 639, 846
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 233
Bpu14I TTCGAA 2 cut(s) 61, 316
BpuEI CTTGAG 2 cut(s) 275, 416
BsaHI GRCGYC 1 cut(s) 688
BsaJI CCNNGG 4 cut(s) 238, 299, 375, 886
BsaWI WCCGGW 2 cut(s) 85, 439
BsaXI ACNNNNNCTCC 6 cut(s) 132, 138, 162, 168, 960, 990
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 487, 893
Bse1I ACTGG 2 cut(s) 169, 244
Bse3DI GCAATG 1 cut(s) 76
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 5 cut(s) 265, 301, 572, 699, 888
BseDI CCNNGG 4 cut(s) 238, 299, 375, 886
BseGI GGATG 4 cut(s) 252, 376, 751, 979
BseLI CCNNNNNNNGG 3 cut(s) 141, 487, 893
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 4 cut(s) 8, 247, 511, 579
BseNI ACTGG 2 cut(s) 169, 244
BseRI GAGGAG 4 cut(s) 278, 632, 647, 825
BseXI GCAGC 2 cut(s) 516, 699
BsgI GTGCAG 1 cut(s) 522
Bsh1236I CGCG 2 cut(s) 527, 986
BshFI GGCC 4 cut(s) 243, 268, 545, 702
BshNI GGYRCC 1 cut(s) 687
BsiSI CCGG 3 cut(s) 86, 440, 785
BslI CCNNNNNNNGG 3 cut(s) 141, 487, 893
BsmI GAATGC 1 cut(s) 718
BsnI GGCC 4 cut(s) 243, 268, 545, 702
Bsp119I TTCGAA 2 cut(s) 61, 316
Bsp1286I GDGCHC 1 cut(s) 364
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 3 cut(s) 537, 669, 814
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 4 cut(s) 525, 665, 716, 986
BspANI GGCC 4 cut(s) 243, 268, 545, 702
BspCNI CTCAG 4 cut(s) 9, 246, 510, 580
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 2 cut(s) 527, 986
BspLI GGNNCC 1 cut(s) 689
BspMAI CTGCAG 1 cut(s) 534
BspPI GGATC 1 cut(s) 545
BspQI GCTCTTC 1 cut(s) 759
BspT104I TTCGAA 2 cut(s) 61, 316
BspT107I GGYRCC 1 cut(s) 687
BsrDI GCAATG 1 cut(s) 76
BsrI ACTGG 2 cut(s) 169, 244
BssECI CCNNGG 4 cut(s) 238, 299, 375, 886
BssMI GATC 3 cut(s) 537, 669, 814
BssNI GRCGYC 1 cut(s) 688
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 5 cut(s) 265, 301, 572, 699, 888
Bst6I CTCTTC 2 cut(s) 443, 759
BstACI GRCGYC 1 cut(s) 688
BstAPI GCANNNNNTGC 1 cut(s) 649
BstBI TTCGAA 2 cut(s) 61, 316
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 4 cut(s) 17, 233, 497, 588
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 4 cut(s) 252, 376, 751, 979
BstFNI CGCG 2 cut(s) 527, 986
BstH2I RGCGCY 1 cut(s) 691
BstHHI GCGC 3 cut(s) 529, 690, 712
BstKTI GATC 3 cut(s) 540, 672, 817
BstMBI GATC 3 cut(s) 537, 669, 814
BstMWI GCNNNNNNNGC 1 cut(s) 649
BstNI CCWGG 5 cut(s) 265, 301, 572, 699, 888
BstSCI CCNGG 5 cut(s) 263, 299, 570, 697, 886
BstSFI CTRYAG 1 cut(s) 530
BstUI CGCG 2 cut(s) 527, 986
BstV1I GCAGC 2 cut(s) 516, 699
BstXI CCANNNNNNTGG 1 cut(s) 698
BsuI GTATCC 2 cut(s) 505, 570
BsuRI GGCC 4 cut(s) 243, 268, 545, 702
BtgI CCRYGG 1 cut(s) 375
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 4 cut(s) 252, 376, 751, 979
BtsI GCAGTG 1 cut(s) 539
BtsIMutI CAGTG 2 cut(s) 505, 539
Cac8I GCNNGC 1 cut(s) 188
CfoI GCGC 3 cut(s) 529, 690, 712
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 1 cut(s) 401
CviAII CATG 3 cut(s) 178, 376, 541
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 4 cut(s) 17, 233, 497, 588
DinI GGCGCC 1 cut(s) 689
DpnI GATC 3 cut(s) 539, 671, 816
DpnII GATC 3 cut(s) 537, 669, 814
DraI TTTAAA 1 cut(s) 660
EaeI YGGCCR 3 cut(s) 241, 543, 700
Eam1104I CTCTTC 2 cut(s) 443, 759
EarI CTCTTC 2 cut(s) 443, 759
Eco130I CCWWGG 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 364
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 214
EcoRII CCWGG 5 cut(s) 263, 299, 570, 697, 886
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 364
EgeI GGCGCC 1 cut(s) 689
EheI GGCGCC 1 cut(s) 689
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 181, 379, 544
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FatI CATG 3 cut(s) 177, 375, 540
Fnu4HI GCNGC 3 cut(s) 530, 713, 716
FokI GGATG 4 cut(s) 239, 383, 758, 986
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 3 cut(s) 530, 713, 716
FspBI CTAG 1 cut(s) 413
GlaI GCGC 3 cut(s) 528, 689, 711
GluI GCNGC 3 cut(s) 530, 713, 716
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 1 cut(s) 691
HaeIII GGCC 4 cut(s) 243, 268, 545, 702
HapII CCGG 3 cut(s) 86, 440, 785
HhaI GCGC 3 cut(s) 529, 690, 712
Hin1I GRCGYC 1 cut(s) 688
Hin1II CATG 3 cut(s) 181, 379, 544
Hin6I GCGC 3 cut(s) 527, 688, 710
HinP1I GCGC 3 cut(s) 527, 688, 710
HincII GTYRAC 2 cut(s) 160, 979
HindII GTYRAC 2 cut(s) 160, 979
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 8 cut(s) 89, 95, 147, 193, 295, 318, 355, 1018
HpaII CCGG 3 cut(s) 86, 440, 785
HphI GGTGA 4 cut(s) 33, 637, 679, 1032
Hpy166II GTNNAC 4 cut(s) 160, 382, 979, 1002
Hpy188I TCNGA 3 cut(s) 94, 323, 354
Hpy188III TCNNGA 4 cut(s) 16, 292, 440, 914
Hpy8I GTNNAC 4 cut(s) 160, 382, 979, 1002
HpyAV CCTTC 4 cut(s) 279, 353, 622, 1021
HpyCH4V TGCA 3 cut(s) 503, 532, 643
HpyF10VI GCNNNNNNNGC 1 cut(s) 649
HpyF3I CTNAG 4 cut(s) 17, 233, 497, 588
Hsp92I GRCGYC 1 cut(s) 688
Hsp92II CATG 3 cut(s) 181, 379, 544
HspAI GCGC 3 cut(s) 527, 688, 710
KasI GGCGCC 1 cut(s) 687
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 3 cut(s) 537, 669, 814
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 1 cut(s) 837
Lsp1109I GCAGC 2 cut(s) 516, 699
LweI GCATC 3 cut(s) 361, 639, 846
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 2 cut(s) 22, 407
MalI GATC 3 cut(s) 539, 671, 816
MboI GATC 3 cut(s) 537, 669, 814
MboII GAAGA 6 cut(s) 338, 347, 460, 658, 776, 889
MhlI GDGCHC 1 cut(s) 364
MlsI TGGCCA 2 cut(s) 545, 702
MluCI AATT 6 cut(s) 9, 31, 259, 564, 931, 944
MluNI TGGCCA 2 cut(s) 545, 702
Mly113I GGCGCC 1 cut(s) 688
MlyI GAGTC 2 cut(s) 83, 156
MmeI TCCRAC 1 cut(s) 159
Mox20I TGGCCA 2 cut(s) 545, 702
MroI TCCGGA 1 cut(s) 439
MscI TGGCCA 2 cut(s) 545, 702
MseI TTAA 7 cut(s) 8, 201, 594, 659, 683, 780, 849
Msp20I TGGCCA 2 cut(s) 545, 702
MspI CCGG 3 cut(s) 86, 440, 785
MspR9I CCNGG 5 cut(s) 265, 301, 572, 699, 888
Mva1269I GAATGC 1 cut(s) 718
MvaI CCWGG 5 cut(s) 265, 301, 572, 699, 888
MvnI CGCG 2 cut(s) 527, 986
MwoI GCNNNNNNNGC 1 cut(s) 649
NarI GGCGCC 1 cut(s) 688
NcoI CCATGG 1 cut(s) 375
NdeII GATC 3 cut(s) 537, 669, 814
NlaIII CATG 3 cut(s) 181, 379, 544
NlaIV GGNNCC 1 cut(s) 689
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 22
NspV TTCGAA 2 cut(s) 61, 316
PciSI GCTCTTC 1 cut(s) 759
PctI GAATGC 1 cut(s) 718
PfeI GAWTC 6 cut(s) 95, 193, 295, 318, 355, 1018
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 3 cut(s) 531, 714, 717
PleI GAGTC 2 cut(s) 83, 155
PluTI GGCGCC 1 cut(s) 691
PpsI GAGTC 2 cut(s) 83, 155
Psp6I CCWGG 5 cut(s) 263, 299, 570, 697, 886
PspGI CCWGG 5 cut(s) 263, 299, 570, 697, 886
PspN4I GGNNCC 1 cut(s) 689
PspPI GGNCC 1 cut(s) 214
PsrI GAACNNNNNNTAC 4 cut(s) 164, 196, 499, 531
PstI CTGCAG 1 cut(s) 534
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 7 cut(s) 8, 201, 594, 659, 683, 780, 849
SatI GCNGC 3 cut(s) 530, 713, 716
Sau3AI GATC 3 cut(s) 537, 669, 814
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 2 cut(s) 83, 156
ScrFI CCNGG 5 cut(s) 265, 301, 572, 699, 888
SduI GDGCHC 1 cut(s) 364
SetI ASST 7 cut(s) 216, 414, 438, 459, 624, 700, 961
SfaNI GCATC 3 cut(s) 361, 639, 846
SfcI CTRYAG 1 cut(s) 530
SfoI GGCGCC 1 cut(s) 689
SfuI TTCGAA 2 cut(s) 61, 316
SinI GGWCC 1 cut(s) 214
SmlI CTYRAG 2 cut(s) 290, 431
SmoI CTYRAG 2 cut(s) 290, 431
Sse9I AATT 6 cut(s) 9, 31, 259, 564, 931, 944
SsiI CCGC 4 cut(s) 525, 665, 716, 986
SspDI GGCGCC 1 cut(s) 687
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 5 cut(s) 263, 299, 570, 697, 886
StyI CCWWGG 1 cut(s) 375
TaqI TCGA 4 cut(s) 61, 316, 358, 948
TasI AATT 6 cut(s) 9, 31, 259, 564, 931, 944
TatI WGTACW 1 cut(s) 400
TauI GCSGC 1 cut(s) 718
TfiI GAWTC 6 cut(s) 95, 193, 295, 318, 355, 1018
Tru1I TTAA 7 cut(s) 8, 201, 594, 659, 683, 780, 849
Tru9I TTAA 7 cut(s) 8, 201, 594, 659, 683, 780, 849
TscAI CASTG 2 cut(s) 505, 539
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 2 cut(s) 529, 712
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 2 cut(s) 261, 899
TspGWI ACGGA 2 cut(s) 153, 524
TspRI CASTG 2 cut(s) 505, 539
VpaK11BI GGWCC 1 cut(s) 214
XapI RAATTY 2 cut(s) 931, 944
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.