MD01G1203800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000853
Physical Location & Seq
Forward (+)
29944278 .. 29945216
939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1203800.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGAAACCTGGTTCCTGATCCTCATCGCCTTCTGCGTCTCCTTCCTCCTCAAACCCCTTCTTTCCCTATTCCTACCCACTTCCATCTCCAAACCCAAGCTCCCTCCTGGGCCCCGCGTCTTCCCCGTCATCGGCGGCTTCTTATTCCTCCTCAAATCCTTCTCGGAGCTCGAGCCTACCATCTGCAAACTCAAGGCCAAATACGGGCCCATCATCTCCCTCCGCCTCGGCTCCCGCCCCGCCGTCTTCATCGCCGACCGCTCCCTCGCCCACCAGGCCTTAATCCAGAACGGCGCCGTGTTCGCCGACCGCCCCCCGGCCTTGGCCACCAACAAGTACGTCACCAGCAACCAGCACAACATCAGCTCCGCCATCTACGGCCCCACTTGGCGCCTCCTCCGCCGCAACCTCACCTCCGAAATCCTTCACCTTTCCCGCATCAAATCCTACGGCAACGCCCGTAAATGGGTTCTCGATATCCTCGTCAACCGCCTCAAAACCGAGTCTCAATCCCACTCCGAAGGCGGCGTCGCCGTCGTCAGGGTGGTCGACCACTTCCAATATGCCATGTTCTGCCTCCTGGTTCTGATGTGCTTCGGCGACAAATTGAACGAAGAGCAAATCAAAGAAATTGAGCGTGTGCATCGCCAACTACTTATGAGTTCCGGGCGGTTCAATATCCTCAATTTCAAGCCGAAATTGACGAAGATTATCTTCAAAAATCGGTGGCGGCAATTCTTCAAAATCCTCGAGGAACGACGAGAAGTGCTCGTCCCTCTGATTCGAGCACGACAAAACAGGGCAGCAAAACCGACGACAAAGACGATGAATTTGTGTTGTCGTATACTGATACGCTGCTGGACCTCGAACTTCCCGACGGCAACGAAAAGCGGAAGCTTTCGGAGGGAGAAACGCTCAGCCTCTGCTCAGAGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

313

Amino Acids

35.76

Weight (kDa)

10.57

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 278 1.6e-28 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 749
AccB1I GGYRCC 2 cut(s) 293, 390
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 2 cut(s) 549, 844
AccII CGCG 1 cut(s) 117
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 1 cut(s) 829
AcyI GRCGYC 3 cut(s) 294, 391, 528
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 7 cut(s) 131, 204, 316, 322, 465, 466, 540
AgsI TTSAA 5 cut(s) 610, 676, 691, 718, 742
AjnI CCWGG 4 cut(s) 8, 106, 273, 579
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 100, 169, 366, 897
AluI AGCT 4 cut(s) 100, 169, 366, 897
Alw21I GWGCWC 3 cut(s) 171, 771, 790
Alw26I GTCTC 2 cut(s) 43, 510
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 923
Ama87I CYCGRG 2 cut(s) 170, 749
AoxI GGCC 7 cut(s) 110, 195, 206, 276, 318, 324, 379
ApaI GGGCCC 2 cut(s) 114, 210
ApeKI GCWGC 2 cut(s) 803, 855
ApoI RAATTY 1 cut(s) 829
ArsI GACNNNNNNTTYG 4 cut(s) 513, 545, 814, 846
Asp700I GAANNNNTTC 1 cut(s) 423
AspLEI GCGC 2 cut(s) 296, 393
AspS9I GGNCC 6 cut(s) 110, 111, 206, 207, 380, 861
AsuC2I CCSGG 2 cut(s) 317, 667
AsuHPI GGTGA 3 cut(s) 334, 403, 419
AvaI CYCGRG 2 cut(s) 170, 749
AvaII GGWCC 1 cut(s) 861
BaeGI GKGCMC 2 cut(s) 114, 210
BalI TGGCCA 1 cut(s) 326
BanI GGYRCC 2 cut(s) 293, 390
BanII GRGCYC 3 cut(s) 114, 171, 210
BbsI GAAGAC 2 cut(s) 112, 238
Bbv12I GWGCWC 3 cut(s) 171, 771, 790
BbvI GCAGC 2 cut(s) 815, 842
BccI CCATC 4 cut(s) 92, 188, 218, 380
BceAI ACGGC 7 cut(s) 227, 281, 307, 394, 466, 518, 894
BciT130I CCWGG 4 cut(s) 10, 108, 275, 581
BcnI CCSGG 2 cut(s) 317, 667
BcoDI GTCTC 2 cut(s) 43, 510
BfoI RGCGCY 2 cut(s) 297, 394
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 6 cut(s) 136, 403, 526, 731, 804, 856
BlpI GCTNAGC 1 cut(s) 916
BlsI GCNGC 6 cut(s) 137, 404, 527, 732, 805, 857
Bme1390I CCNGG 6 cut(s) 10, 108, 275, 317, 581, 667
Bme18I GGWCC 1 cut(s) 861
BmeT110I CYCGRG 2 cut(s) 170, 749
BmgT120I GGNCC 6 cut(s) 110, 111, 206, 207, 380, 861
BmiI GGNNCC 8 cut(s) 14, 112, 113, 208, 232, 295, 382, 392
BmrFI CCNGG 6 cut(s) 10, 108, 275, 317, 581, 667
BmsI GCATC 2 cut(s) 447, 652
BpiI GAAGAC 2 cut(s) 112, 238
BplI GAGNNNNNCTC 4 cut(s) 753, 785, 899, 931
Bpu1102I GCTNAGC 1 cut(s) 916
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 2 cut(s) 317, 667
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 3 cut(s) 294, 391, 528
BsaJI CCNNGG 4 cut(s) 107, 226, 315, 321
BsaXI ACNNNNNCTCC 6 cut(s) 84, 114, 350, 380, 398, 428
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 204, 316, 322, 465, 466, 540
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 4 cut(s) 10, 108, 275, 581
BseDI CCNNGG 4 cut(s) 107, 226, 315, 321
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 7 cut(s) 131, 204, 316, 322, 465, 466, 540
BseMII CTCAG 1 cut(s) 930
BseRI GAGGAG 3 cut(s) 38, 140, 386
BseSI GKGCMC 2 cut(s) 114, 210
BseXI GCAGC 2 cut(s) 815, 842
Bsh1236I CGCG 1 cut(s) 117
Bsh1285I CGRYCG 2 cut(s) 259, 310
BshFI GGCC 7 cut(s) 112, 197, 208, 278, 320, 326, 381
BshNI GGYRCC 2 cut(s) 293, 390
BsiEI CGRYCG 2 cut(s) 259, 310
BsiHKAI GWGCWC 3 cut(s) 171, 771, 790
BsiHKCI CYCGRG 2 cut(s) 170, 749
BsiSI CCGG 2 cut(s) 317, 666
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 7 cut(s) 131, 204, 316, 322, 465, 466, 540
BsmAI GTCTC 2 cut(s) 43, 510
BsmBI CGTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 7 cut(s) 112, 197, 208, 278, 320, 326, 381
BsoBI CYCGRG 2 cut(s) 170, 749
Bsp120I GGGCCC 2 cut(s) 110, 206
Bsp1286I GDGCHC 5 cut(s) 114, 171, 210, 771, 790
Bsp143I GATC 1 cut(s) 18
Bsp1720I GCTNAGC 1 cut(s) 916
BspANI GGCC 7 cut(s) 112, 197, 208, 278, 320, 326, 381
BspCNI CTCAG 1 cut(s) 929
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 8 cut(s) 14, 112, 113, 208, 232, 295, 382, 392
BspPI GGATC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 609
BspT107I GGYRCC 2 cut(s) 293, 390
BsrBI CCGCTC 1 cut(s) 261
BssECI CCNNGG 4 cut(s) 107, 226, 315, 321
BssMI GATC 1 cut(s) 18
BssNAI GTATAC 1 cut(s) 845
BssNI GRCGYC 3 cut(s) 294, 391, 528
BssT1I CCWWGG 1 cut(s) 321
Bst1107I GTATAC 1 cut(s) 845
Bst2UI CCWGG 4 cut(s) 10, 108, 275, 581
Bst6I CTCTTC 1 cut(s) 609
BstACI GRCGYC 3 cut(s) 294, 391, 528
BstDEI CTNAG 2 cut(s) 916, 927
BstFNI CGCG 1 cut(s) 117
BstH2I RGCGCY 2 cut(s) 297, 394
BstHHI GCGC 2 cut(s) 296, 393
BstKTI GATC 1 cut(s) 21
BstMAI GTCTC 2 cut(s) 43, 510
BstMBI GATC 1 cut(s) 18
BstMCI CGRYCG 2 cut(s) 259, 310
BstMWI GCNNNNNNNGC 3 cut(s) 275, 302, 399
BstNI CCWGG 4 cut(s) 10, 108, 275, 581
BstSCI CCNGG 6 cut(s) 8, 106, 273, 315, 579, 665
BstSLI GKGCMC 2 cut(s) 114, 210
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 2 cut(s) 815, 842
BstV2I GAAGAC 2 cut(s) 112, 238
BstZ17I GTATAC 1 cut(s) 845
BsuRI GGCC 7 cut(s) 112, 197, 208, 278, 320, 326, 381
BtgZI GCGATG 3 cut(s) 10, 235, 629
CaiI CAGNNNCTG 1 cut(s) 923
CfoI GCGC 2 cut(s) 296, 393
Cfr13I GGNCC 6 cut(s) 110, 111, 206, 207, 380, 861
CseI GACGC 3 cut(s) 25, 106, 517
CsiI ACCWGGT 1 cut(s) 8
Csp6I GTAC 1 cut(s) 337
CviAII CATG 1 cut(s) 568
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 2 cut(s) 916, 927
DinI GGCGCC 2 cut(s) 295, 392
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
EciI GGCGGA 3 cut(s) 212, 358, 389
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 321
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 3 cut(s) 114, 171, 210
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 861
Eco53kI GAGCTC 1 cut(s) 169
Eco88I CYCGRG 2 cut(s) 170, 749
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 111
EcoRII CCWGG 4 cut(s) 8, 106, 273, 579
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 321
EcoT38I GRGCYC 3 cut(s) 114, 171, 210
EgeI GGCGCC 2 cut(s) 295, 392
EheI GGCGCC 2 cut(s) 295, 392
ErhI CCWWGG 1 cut(s) 321
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 1 cut(s) 571
FaiI YATR 4 cut(s) 564, 569, 659, 845
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FaqI GGGAC 1 cut(s) 758
FatI CATG 1 cut(s) 567
FauI CCCGC 4 cut(s) 122, 242, 247, 443
FblI GTMKAC 2 cut(s) 549, 844
Fnu4HI GCNGC 6 cut(s) 136, 403, 526, 731, 804, 856
FriOI GRGCYC 3 cut(s) 114, 171, 210
Fsp4HI GCNGC 6 cut(s) 136, 403, 526, 731, 804, 856
GlaI GCGC 2 cut(s) 295, 392
GluI GCNGC 6 cut(s) 136, 403, 526, 731, 804, 856
HaeII RGCGCY 2 cut(s) 297, 394
HaeIII GGCC 7 cut(s) 112, 197, 208, 278, 320, 326, 381
HapII CCGG 2 cut(s) 317, 666
HgaI GACGC 3 cut(s) 25, 106, 517
HhaI GCGC 2 cut(s) 296, 393
Hin1I GRCGYC 3 cut(s) 294, 391, 528
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 2 cut(s) 294, 391
HinP1I GCGC 2 cut(s) 294, 391
HincII GTYRAC 2 cut(s) 487, 550
HindII GTYRAC 2 cut(s) 487, 550
HindIII AAGCTT 1 cut(s) 895
HinfI GANTC 2 cut(s) 503, 781
HpaII CCGG 2 cut(s) 317, 666
HphI GGTGA 3 cut(s) 334, 403, 419
Hpy166II GTNNAC 3 cut(s) 487, 550, 845
Hpy188I TCNGA 8 cut(s) 166, 418, 520, 588, 780, 903, 930, 938
Hpy188III TCNNGA 4 cut(s) 16, 286, 473, 874
Hpy8I GTNNAC 3 cut(s) 487, 550, 845
Hpy99I CGWCG 5 cut(s) 533, 539, 762, 817, 880
HpyAV CCTTC 6 cut(s) 40, 52, 68, 169, 434, 515
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 2 cut(s) 186, 643
HpyF10VI GCNNNNNNNGC 3 cut(s) 275, 302, 399
HpyF3I CTNAG 2 cut(s) 916, 927
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 3 cut(s) 294, 391, 528
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 2 cut(s) 294, 391
KasI GGCGCC 2 cut(s) 293, 390
Kzo9I GATC 1 cut(s) 18
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 5 cut(s) 105, 166, 236, 266, 371
Lsp1109I GCAGC 2 cut(s) 815, 842
LweI GCATC 2 cut(s) 447, 652
MabI ACCWGGT 1 cut(s) 8
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 340
MalI GATC 1 cut(s) 20
MbiI CCGCTC 1 cut(s) 261
MboI GATC 1 cut(s) 18
MboII GAAGA 6 cut(s) 112, 238, 626, 706, 718, 730
MhlI GDGCHC 5 cut(s) 114, 171, 210, 771, 790
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 6 cut(s) 605, 630, 685, 698, 734, 829
MluNI TGGCCA 1 cut(s) 326
Mly113I GGCGCC 2 cut(s) 294, 391
MlyI GAGTC 1 cut(s) 512
Mox20I TGGCCA 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 423
MscI TGGCCA 1 cut(s) 326
MseI TTAA 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 326
MspI CCGG 2 cut(s) 317, 666
MspR9I CCNGG 6 cut(s) 10, 108, 275, 317, 581, 667
MvaI CCWGG 4 cut(s) 10, 108, 275, 581
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 3 cut(s) 275, 302, 399
NarI GGCGCC 2 cut(s) 294, 391
NciI CCSGG 2 cut(s) 317, 667
NdeII GATC 1 cut(s) 18
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 8 cut(s) 14, 112, 113, 208, 232, 295, 382, 392
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 340
PaeR7I CTCGAG 2 cut(s) 170, 749
PceI AGGCCT 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 609
PcsI WCGNNNNNNNCGW 4 cut(s) 33, 480, 821, 872
PdmI GAANNNNTTC 1 cut(s) 423
PfeI GAWTC 1 cut(s) 781
PkrI GCNGC 6 cut(s) 137, 404, 527, 732, 805, 857
PleI GAGTC 1 cut(s) 511
PluTI GGCGCC 2 cut(s) 297, 394
PpsI GAGTC 1 cut(s) 511
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 4 cut(s) 8, 106, 273, 579
PspGI CCWGG 4 cut(s) 8, 106, 273, 579
PspN4I GGNNCC 8 cut(s) 14, 112, 113, 208, 232, 295, 382, 392
PspOMI GGGCCC 2 cut(s) 110, 206
PspPI GGNCC 6 cut(s) 110, 111, 206, 207, 380, 861
PspXI VCTCGAGB 2 cut(s) 170, 749
PstNI CAGNNNCTG 1 cut(s) 923
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 548
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 6 cut(s) 136, 403, 526, 731, 804, 856
Sau3AI GATC 1 cut(s) 18
Sau96I GGNCC 6 cut(s) 110, 111, 206, 207, 380, 861
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 6 cut(s) 10, 108, 275, 317, 581, 667
SduI GDGCHC 5 cut(s) 114, 171, 210, 771, 790
SexAI ACCWGGT 1 cut(s) 8
SfaNI GCATC 2 cut(s) 447, 652
SfoI GGCGCC 2 cut(s) 295, 392
Sfr274I CTCGAG 2 cut(s) 170, 749
SinI GGWCC 1 cut(s) 861
SlaI CTCGAG 2 cut(s) 170, 749
SmlI CTYRAG 3 cut(s) 170, 191, 749
SmoI CTYRAG 3 cut(s) 170, 191, 749
Sse9I AATT 6 cut(s) 605, 630, 685, 698, 734, 829
SseBI AGGCCT 1 cut(s) 278
SspDI GGCGCC 2 cut(s) 293, 390
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 6 cut(s) 8, 106, 273, 315, 579, 665
StyI CCWWGG 1 cut(s) 321
TaiI ACGT 1 cut(s) 342
TaqI TCGA 6 cut(s) 171, 474, 549, 750, 784, 866
TasI AATT 6 cut(s) 605, 630, 685, 698, 734, 829
TauI GCSGC 4 cut(s) 138, 405, 528, 733
TfiI GAWTC 1 cut(s) 781
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 803, 855
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 2 cut(s) 238, 842
VpaK11BI GGWCC 1 cut(s) 861
XapI RAATTY 1 cut(s) 829
XhoI CTCGAG 2 cut(s) 170, 749
XmiI GTMKAC 2 cut(s) 549, 844
XmnI GAANNNNTTC 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.