FvH4_7g29720

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000853
Physical Location & Seq
Forward (+)
21806833 .. 21808481
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29720.t1

Sequence Viewer

Length: 1521 bp
ATGGAAACCTGGCTCCTCATTCTCACCACCCTCCTCATCTCTCTCCTCATCAGACCCCTTCTCTCCCTCCTCCTCAAACCCAAGAACAACCTCCCGCCGGGACCCACAAACTTCCCCATCATCACCAGCTCCCTCTGGCTCCTCAAACCCTTCTCCGACCTCGAGCCCGCCCTCCGCCGCCTCCAATCTCGCTACGGCCCAATCCTCACCCTCCCCATCGGCTCCCGTCCCGCCATTTTCATCTCCTCCCCCTCCCTGGCCCGCCAGGCCTTAATCCAAAACGGCGCCGTCTTCGCCGACCGCCCACCTTTCCTGCTCACCGACAGAATCATCAGCAGCAACCAGCACACTATCAGCTCCGCCGTCTACGGCCCCACGTGGCGCCTCCTCCGCCGTAACCTCACCCACGAAATCCTCCACCCTTCCCGCGTCAAATCCTACACCGCCGCCCGCAACTGGGTCCTCCACGTCCTCGCCAAACGTCTAACAGAGTCCCAGTCCAGCTCCGTCGCCGGAGTCAAAGTCGTTGACCATTTGCAGTACGCCATGTTCTGCCTGCTCGTTCTTATGTGCTTCGGAGACAAACTAGACGAGACTCAGATCAAGCAAATAGAGCAGGTCCAGCGTAAGCTTCTGATGAACTCGACACAGTTCAACGTCATCAACTTCTTCCCTGCACTGACGAAAATCGTGCTCAAGAAGAAGTGGGCCGAGTTTCTCCGAATGCGAAAAGAGCAGGAAGATGTGCTTCTTCCTCTGATCAGAGCACGAAAGGAAGCAAAGAAGCTAGGTAGTGATGATGATCATGTGCTTTGTTACGTGGACACGCTCTGGGAGCTGGAGCTGCCTGAAGAGAAGAGGAAGCTGACGGAAGACGAGATCGTTAGTCTTTGCTCGGAGTTTCTCAATGCGGGTACAGATACTACTTCGACTGCGTTGCAGTGGATCATGGCCAATATAGTGAAGTATCGAGATGTGCAGGAGAGGCTTGTGGCGGAGATTAAAGGAGTTGTGGAGGAAACAGAGGAGGAGGTGAAGGAGGAGGACTTGCAGAAGATGCCTTATTTGAAGGCGGTGATATTGGAAGGGCTGAGGCGTCACCCGCCAGGACATTTCGTGCTGCCGCACGCGGTGACGCAGGACGTGGTTTTGGATGGTTATGTGGTGCCGAAGAAGGGGATGGTGAATTTCATGGTGGCGGATATAGGGTGGGATGAGAAAGTATGGGAGGAGCCTATGGAGTTTAAGCCGGAGAGGTTTTTGAGTGGTGGTGAAGGAGAAGGGGTGGATATAACTGGGAGTAGGGAGATTAAGATGATGCCGTTTGGAGTTGGGAGGAGGATATGTCCTGGACTTGGTTTGGCAGTGTTGCATTTAGAGTATTTCGTGGCGAATCTTGTTTGGAAATTTGAATGGAATACTGTGGAGGGTGAGGATGTTGATTTTGCAGAGAAGCAAGAGTTCACTATGGTCATGAAGAATCCATTGAAGGCTCACATTTCACCACGAGTGATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

507

Amino Acids

57.8

Weight (kDa)

7.18

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 32 - 497 2e-97 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 607
AccB1I GGYRCC 3 cut(s) 284, 381, 1165
AccI GTMKAC 1 cut(s) 366
AccII CGCG 2 cut(s) 429, 1130
AclWI GGATC 1 cut(s) 953
AcoI YGGCCR 1 cut(s) 951
AcsI RAATTY 2 cut(s) 1186, 1406
AcuI CTGAAG 1 cut(s) 870
AcvI CACGTG 1 cut(s) 378
AcyI GRCGYC 3 cut(s) 285, 382, 1096
AdeI CACNNNGTG 1 cut(s) 1132
AfaI GTAC 2 cut(s) 542, 916
AfiI CCNNNNNNNGG 6 cut(s) 97, 256, 456, 457, 1175, 1355
AgsI TTSAA 4 cut(s) 655, 1069, 1412, 1489
AjiI CACGTC 2 cut(s) 469, 1144
AjnI CCWGG 5 cut(s) 8, 255, 264, 1105, 1348
AluBI AGCT 8 cut(s) 129, 357, 504, 631, 787, 838, 844, 865
AluI AGCT 8 cut(s) 129, 357, 504, 631, 787, 838, 844, 865
Alw21I GWGCWC 2 cut(s) 696, 769
Alw26I GTCTC 2 cut(s) 573, 587
AlwI GGATC 1 cut(s) 953
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 6 cut(s) 196, 258, 267, 370, 708, 951
ApeKI GCWGC 3 cut(s) 336, 844, 1120
ApoI RAATTY 2 cut(s) 1186, 1406
AspLEI GCGC 2 cut(s) 287, 384
AspS9I GGNCC 7 cut(s) 101, 197, 259, 371, 460, 619, 708
AsuC2I CCSGG 1 cut(s) 99
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 3 cut(s) 101, 460, 619
BalI TGGCCA 1 cut(s) 953
BanI GGYRCC 3 cut(s) 284, 381, 1165
BanII GRGCYC 1 cut(s) 168
BauI CACGAG 1 cut(s) 1506
BbrPI CACGTG 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 283, 879
Bbv12I GWGCWC 2 cut(s) 696, 769
BbvCI CCTCAGC 1 cut(s) 1091
BbvI GCAGC 3 cut(s) 348, 831, 1107
BccI CCATC 4 cut(s) 125, 224, 1148, 1174
BceAI ACGGC 7 cut(s) 211, 272, 298, 347, 378, 385, 1306
BcgI CGANNNNNNTGC 4 cut(s) 673, 707, 919, 953
BciT130I CCWGG 5 cut(s) 10, 257, 266, 1107, 1350
BclI TGATCA 2 cut(s) 759, 802
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 2 cut(s) 573, 587
BfaI CTAG 2 cut(s) 587, 788
BfoI RGCGCY 2 cut(s) 288, 385
BfuAI ACCTGC 1 cut(s) 607
BglI GCCNNNNNGGC 1 cut(s) 266
BisI GCNGC 6 cut(s) 178, 337, 447, 845, 1121, 1124
BlsI GCNGC 6 cut(s) 179, 338, 448, 846, 1122, 1125
Bme1390I CCNGG 6 cut(s) 10, 99, 257, 266, 1107, 1350
Bme18I GGWCC 3 cut(s) 101, 460, 619
BmeT110I CYCGRG 1 cut(s) 161
BmgBI CACGTC 2 cut(s) 469, 1144
BmgT120I GGNCC 7 cut(s) 101, 197, 259, 371, 460, 619, 708
BmrFI CCNGG 6 cut(s) 10, 99, 257, 266, 1107, 1350
BmrI ACTGGG 3 cut(s) 466, 490, 1305
BmsI GCATC 2 cut(s) 1047, 1308
BmuI ACTGGG 3 cut(s) 466, 490, 1305
BpiI GAAGAC 2 cut(s) 283, 879
BpmI CTGGAG 1 cut(s) 860
Bpu10I CCTNAGC 1 cut(s) 1091
BpuEI CTTGAG 1 cut(s) 680
BpuMI CCSGG 1 cut(s) 99
BsaAI YACGTR 2 cut(s) 378, 820
BsaBI GATNNNNATC 1 cut(s) 801
BsaHI GRCGYC 3 cut(s) 285, 382, 1096
BsaJI CCNNGG 1 cut(s) 255
BsaXI ACNNNNNCTCC 6 cut(s) 507, 537, 1269, 1299, 1330, 1360
Bsc4I CCNNNNNNNGG 6 cut(s) 97, 256, 456, 457, 1175, 1355
Bse1I ACTGG 3 cut(s) 461, 496, 1300
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 5 cut(s) 10, 257, 266, 1107, 1350
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 4 cut(s) 1159, 1185, 1219, 1441
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 6 cut(s) 97, 256, 456, 457, 1175, 1355
BseMII CTCAG 2 cut(s) 611, 1082
BseNI ACTGG 3 cut(s) 461, 496, 1300
BseXI GCAGC 3 cut(s) 348, 831, 1107
BsgI GTGCAG 2 cut(s) 660, 998
Bsh1236I CGCG 2 cut(s) 429, 1130
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 6 cut(s) 198, 260, 269, 372, 710, 953
BshNI GGYRCC 3 cut(s) 284, 381, 1165
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 2 cut(s) 696, 769
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 98, 513, 1250
BslFI GGGAC 3 cut(s) 114, 213, 478
BslI CCNNNNNNNGG 6 cut(s) 97, 256, 456, 457, 1175, 1355
BsmAI GTCTC 2 cut(s) 573, 587
BsmFI GGGAC 3 cut(s) 114, 213, 478
BsmI GAATGC 1 cut(s) 729
BsnI GGCC 6 cut(s) 198, 260, 269, 372, 710, 953
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 3 cut(s) 168, 696, 769
Bsp143I GATC 5 cut(s) 600, 759, 802, 879, 945
BspANI GGCC 6 cut(s) 198, 260, 269, 372, 710, 953
BspCNI CTCAG 2 cut(s) 610, 1083
BspFNI CGCG 2 cut(s) 429, 1130
BspHI TCATGA 1 cut(s) 1473
BspMI ACCTGC 1 cut(s) 607
BspPI GGATC 1 cut(s) 953
BspT107I GGYRCC 3 cut(s) 284, 381, 1165
BsrI ACTGG 3 cut(s) 461, 496, 1300
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 5 cut(s) 600, 759, 802, 879, 945
BssNI GRCGYC 3 cut(s) 285, 382, 1096
BssSI CACGAG 1 cut(s) 1506
Bst2BI CACGAG 1 cut(s) 1506
Bst2UI CCWGG 5 cut(s) 10, 257, 266, 1107, 1350
Bst4CI ACNGT 2 cut(s) 651, 1423
Bst6I CTCTTC 2 cut(s) 846, 851
BstACI GRCGYC 3 cut(s) 285, 382, 1096
BstAPI GCANNNNNTGC 1 cut(s) 1057
BstBAI YACGTR 2 cut(s) 378, 820
BstC8I GCNNGC 5 cut(s) 168, 262, 451, 557, 1128
BstDEI CTNAG 2 cut(s) 597, 1091
BstF5I GGATG 4 cut(s) 1159, 1185, 1219, 1441
BstFNI CGCG 2 cut(s) 429, 1130
BstH2I RGCGCY 2 cut(s) 288, 385
BstHHI GCGC 2 cut(s) 287, 384
BstKTI GATC 5 cut(s) 603, 762, 805, 882, 948
BstMAI GTCTC 2 cut(s) 573, 587
BstMBI GATC 5 cut(s) 600, 759, 802, 879, 945
BstMCI CGRYCG 1 cut(s) 301
BstNI CCWGG 5 cut(s) 10, 257, 266, 1107, 1350
BstSCI CCNGG 6 cut(s) 8, 97, 255, 264, 1105, 1348
BstUI CGCG 2 cut(s) 429, 1130
BstV1I GCAGC 3 cut(s) 348, 831, 1107
BstV2I GAAGAC 2 cut(s) 283, 879
BsuRI GGCC 6 cut(s) 198, 260, 269, 372, 710, 953
BtrI CACGTC 2 cut(s) 469, 1144
BtsCI GGATG 4 cut(s) 1159, 1185, 1219, 1441
BtsI GCAGTG 2 cut(s) 947, 1371
BtsIMutI CAGTG 3 cut(s) 677, 947, 1371
BveI ACCTGC 1 cut(s) 607
Cac8I GCNNGC 5 cut(s) 168, 262, 451, 557, 1128
CciI TCATGA 1 cut(s) 1473
CfoI GCGC 2 cut(s) 287, 384
Cfr13I GGNCC 7 cut(s) 101, 197, 259, 371, 460, 619, 708
CseI GACGC 3 cut(s) 418, 1085, 1144
Csp6I GTAC 2 cut(s) 541, 915
CviAII CATG 5 cut(s) 547, 806, 949, 1192, 1474
CviQI GTAC 2 cut(s) 541, 915
DdeI CTNAG 2 cut(s) 597, 1091
DinI GGCGCC 2 cut(s) 286, 383
DpnI GATC 5 cut(s) 602, 761, 804, 881, 947
DpnII GATC 5 cut(s) 600, 759, 802, 879, 945
DraIII CACNNNGTG 1 cut(s) 1132
EaeI YGGCCR 1 cut(s) 951
Eam1104I CTCTTC 2 cut(s) 846, 851
EarI CTCTTC 2 cut(s) 846, 851
EciI GGCGGA 5 cut(s) 164, 349, 380, 1010, 1214
Eco147I AGGCCT 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 3 cut(s) 101, 460, 619
Eco57I CTGAAG 1 cut(s) 870
Eco72I CACGTG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 161
EcoO109I RGGNCCY 2 cut(s) 101, 460
EcoRII CCWGG 5 cut(s) 8, 255, 264, 1105, 1348
EcoT38I GRGCYC 1 cut(s) 168
EgeI GGCGCC 2 cut(s) 286, 383
EheI GGCGCC 2 cut(s) 286, 383
FaeI CATG 5 cut(s) 550, 809, 952, 1195, 1477
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 3 cut(s) 114, 213, 478
FatI CATG 5 cut(s) 546, 805, 948, 1191, 1473
FauI CCCGC 8 cut(s) 102, 175, 238, 269, 434, 458, 904, 1110
FbaI TGATCA 2 cut(s) 759, 802
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 6 cut(s) 178, 337, 447, 845, 1121, 1124
FokI GGATG 4 cut(s) 1166, 1192, 1226, 1448
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 6 cut(s) 178, 337, 447, 845, 1121, 1124
FspBI CTAG 2 cut(s) 587, 788
GlaI GCGC 2 cut(s) 286, 383
GluI GCNGC 6 cut(s) 178, 337, 447, 845, 1121, 1124
GsuI CTGGAG 1 cut(s) 860
HaeII RGCGCY 2 cut(s) 288, 385
HaeIII GGCC 6 cut(s) 198, 260, 269, 372, 710, 953
HapII CCGG 3 cut(s) 98, 513, 1250
HgaI GACGC 3 cut(s) 418, 1085, 1144
HhaI GCGC 2 cut(s) 287, 384
Hin1I GRCGYC 3 cut(s) 285, 382, 1096
Hin1II CATG 5 cut(s) 550, 809, 952, 1195, 1477
Hin6I GCGC 2 cut(s) 285, 382
HinP1I GCGC 2 cut(s) 285, 382
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 6 cut(s) 327, 491, 516, 595, 1393, 1480
HpaII CCGG 3 cut(s) 98, 513, 1250
Hpy166II GTNNAC 4 cut(s) 367, 529, 823, 1464
Hpy188I TCNGA 9 cut(s) 53, 157, 578, 600, 636, 722, 759, 764, 898
Hpy188III TCNNGA 3 cut(s) 697, 971, 1474
Hpy8I GTNNAC 4 cut(s) 367, 529, 823, 1464
Hpy99I CGWCG 1 cut(s) 512
HpyCH4III ACNGT 2 cut(s) 651, 1423
HpyCH4IV ACGT 6 cut(s) 377, 468, 481, 657, 819, 1143
HpyCH4V TGCA 7 cut(s) 538, 677, 940, 979, 1051, 1372, 1448
HpyF3I CTNAG 2 cut(s) 597, 1091
HpySE526I ACGT 6 cut(s) 377, 468, 481, 657, 819, 1143
Hsp92I GRCGYC 3 cut(s) 285, 382, 1096
Hsp92II CATG 5 cut(s) 550, 809, 952, 1195, 1477
HspAI GCGC 2 cut(s) 285, 382
KasI GGCGCC 2 cut(s) 284, 381
KflI GGGWCCC 1 cut(s) 101
Ksp22I TGATCA 2 cut(s) 759, 802
Kzo9I GATC 5 cut(s) 600, 759, 802, 879, 945
LmnI GCTCC 9 cut(s) 18, 134, 144, 227, 362, 509, 835, 841, 1231
Lsp1109I GCAGC 3 cut(s) 348, 831, 1107
LweI GCATC 2 cut(s) 1047, 1308
MaeI CTAG 2 cut(s) 587, 788
MaeII ACGT 6 cut(s) 377, 468, 481, 657, 819, 1143
MaeIII GTNAC 4 cut(s) 395, 815, 1097, 1132
MalI GATC 5 cut(s) 602, 761, 804, 881, 947
MboI GATC 5 cut(s) 600, 759, 802, 879, 945
MhlI GDGCHC 3 cut(s) 168, 696, 769
MlsI TGGCCA 1 cut(s) 953
MluCI AATT 2 cut(s) 1186, 1406
MluNI TGGCCA 1 cut(s) 953
Mly113I GGCGCC 2 cut(s) 285, 382
MlyI GAGTC 3 cut(s) 500, 525, 589
MmeI TCCRAC 1 cut(s) 180
Mox20I TGGCCA 1 cut(s) 953
MscI TGGCCA 1 cut(s) 953
MseI TTAA 4 cut(s) 272, 1002, 1245, 1311
Msp20I TGGCCA 1 cut(s) 953
MspI CCGG 3 cut(s) 98, 513, 1250
MspR9I CCNGG 6 cut(s) 10, 99, 257, 266, 1107, 1350
Mva1269I GAATGC 1 cut(s) 729
MvaI CCWGG 5 cut(s) 10, 257, 266, 1107, 1350
MvnI CGCG 2 cut(s) 429, 1130
NarI GGCGCC 2 cut(s) 285, 382
NciI CCSGG 1 cut(s) 99
NdeII GATC 5 cut(s) 600, 759, 802, 879, 945
NlaIII CATG 5 cut(s) 550, 809, 952, 1195, 1477
NmeAIII GCCGAG 1 cut(s) 736
NmuCI GTSAC 2 cut(s) 1097, 1132
PaeR7I CTCGAG 1 cut(s) 161
PagI TCATGA 1 cut(s) 1473
PceI AGGCCT 1 cut(s) 269
PctI GAATGC 1 cut(s) 729
PfeI GAWTC 3 cut(s) 327, 1393, 1480
PfoI TCCNGGA 1 cut(s) 1348
PkrI GCNGC 6 cut(s) 179, 338, 448, 846, 1122, 1125
PleI GAGTC 3 cut(s) 499, 524, 589
PluTI GGCGCC 2 cut(s) 288, 385
PmaCI CACGTG 1 cut(s) 378
PmlI CACGTG 1 cut(s) 378
PpsI GAGTC 3 cut(s) 499, 524, 589
Ppu21I YACGTR 2 cut(s) 378, 820
PpuMI RGGWCCY 2 cut(s) 101, 460
Psp5II RGGWCCY 2 cut(s) 101, 460
Psp6I CCWGG 5 cut(s) 8, 255, 264, 1105, 1348
PspCI CACGTG 1 cut(s) 378
PspGI CCWGG 5 cut(s) 8, 255, 264, 1105, 1348
PspPI GGNCC 7 cut(s) 101, 197, 259, 371, 460, 619, 708
PspPPI RGGWCCY 2 cut(s) 101, 460
PspXI VCTCGAGB 1 cut(s) 161
PsrI GAACNNNNNNTAC 2 cut(s) 533, 565
RsaI GTAC 2 cut(s) 542, 916
RsaNI GTAC 2 cut(s) 541, 915
SaqAI TTAA 4 cut(s) 272, 1002, 1245, 1311
SatI GCNGC 6 cut(s) 178, 337, 447, 845, 1121, 1124
Sau3AI GATC 5 cut(s) 600, 759, 802, 879, 945
Sau96I GGNCC 7 cut(s) 101, 197, 259, 371, 460, 619, 708
SchI GAGTC 3 cut(s) 500, 525, 589
ScrFI CCNGG 6 cut(s) 10, 99, 257, 266, 1107, 1350
SduI GDGCHC 3 cut(s) 168, 696, 769
SfaNI GCATC 2 cut(s) 1047, 1308
SfiI GGCCNNNNNGGCC 1 cut(s) 266
SfoI GGCGCC 2 cut(s) 286, 383
Sfr274I CTCGAG 1 cut(s) 161
SinI GGWCC 3 cut(s) 101, 460, 619
SlaI CTCGAG 1 cut(s) 161
SmlI CTYRAG 2 cut(s) 161, 695
SmoI CTYRAG 2 cut(s) 161, 695
Sse9I AATT 2 cut(s) 1186, 1406
SseBI AGGCCT 1 cut(s) 269
SspDI GGCGCC 2 cut(s) 284, 381
SspMI CTAG 2 cut(s) 587, 788
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 6 cut(s) 8, 97, 255, 264, 1105, 1348
TaaI ACNGT 2 cut(s) 651, 1423
TaiI ACGT 6 cut(s) 380, 471, 484, 660, 822, 1146
TaqI TCGA 4 cut(s) 162, 644, 929, 970
TasI AATT 2 cut(s) 1186, 1406
TauI GCSGC 3 cut(s) 180, 449, 1126
TfiI GAWTC 3 cut(s) 327, 1393, 1480
Tru1I TTAA 4 cut(s) 272, 1002, 1245, 1311
Tru9I TTAA 4 cut(s) 272, 1002, 1245, 1311
TscAI CASTG 3 cut(s) 684, 947, 1371
TseFI GTSAC 2 cut(s) 1097, 1132
TseI GCWGC 3 cut(s) 336, 844, 1120
Tsp45I GTSAC 2 cut(s) 1097, 1132
TspDTI ATGAA 4 cut(s) 229, 653, 1180, 1490
TspGWI ACGGA 2 cut(s) 496, 884
TspRI CASTG 3 cut(s) 684, 947, 1371
VpaK11BI GGWCC 3 cut(s) 101, 460, 619
XapI RAATTY 2 cut(s) 1186, 1406
XhoI CTCGAG 1 cut(s) 161
XmiI GTMKAC 1 cut(s) 366
XspI CTAG 2 cut(s) 587, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.