MD01G1204500.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000853
Physical Location & Seq
Forward (+)
30012555 .. 30019322
6768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1204500.v1.1.491

Sequence Viewer

Length: 3291 bp
ATGGAAACCTGGTTCCTTATCCTCACCGCCCTCTGCGTCTCCTTCCTCCTCAAAGCCCTTCTTTCTCTATTCCTACCCACTTCCATCTCCAAACCCAAGCCCCCTCCTGGGCCCCGCTACATCCCCATCATCAGCACCTTGTTATTCCTCCTCAAATCCTTCTCGGAGCTCGAGCCTATCATCCGCAAACTACAGGCCAAACACGGGCCCATCATCTCCCTCCGCTTCGGCTCCCGCTCCGCCGTCTTCATCGGCGACCGCTCCCTCGCCCACCAAGCCTTAATCCAGAACGGTGCCGTGTTCGCCGACCGCCCCCGGGCCTTGGCCACCAACGAGTACGTCAGCAGCAACCAGCACAACATCAGCTCCGCCCGATACGGCCCCACTTGGCGCCTCCTCCGCCGCAACCTCACTTCCGAAATCCTCCACCCTTCCCGCGTCAAATCATACGGCAACGCCCGCAAATGGGTTCTCGACATCCTAGTCAACCGCCTCAAAACCGAGTCTCAATCCCAGTCCGAATGCGGCGGCGGCGGTGGTGTCAGGGTGGTCGACCACTTCCAATACGCCATGTTCTGCCTCCTGGTTCTGATGTGCTTCGGTGACAAATTGAACGAAGAGCAAATCAAAGAAATCGAGCGCGTTCAGCGCCAACAACTTTTGAGTTTCGGGCGGTTCAACATCCTCAATTTCAAGCCGAAATTGACCAAGATTCTCTTAAAAAATCGGTGGCGGCAATTCTTCAAAATCCTCGAGGAACAACGAGAAGTGCTCGTCCCTCTGATTCGAGCACGACAAAACAGGGCAAAACAGGGCAGCAAAAACGATGACAAAGACGATGAATTTGTGTTGTCGTACACTGATACGCTGCTGGACCTCGAGCTTCCCGACGGCAACGAAAAGCGGAAGCTTTCGGAGGGAGAAATGGTCAGCCTCTGCTCAGAGTTTCTGAACGCCGGCACCGACACGACTTCCACCGCACTGCAGTGGATCATGGCCAACGTAGTCAAGTACCCACAAGTTCAGGAGAAGCTGTTTGCGGAGATTAAAGGGGTTATGGGGGAAACGGAGGAGGAGGTGCGGGAGGAGGTCCTGCATAAGCTGCTGTATCTGAAAGCCGTGATCTTGGAGGGGCTGAGGCGCCACCCGCCGGGGCACTTTGTGCTGCCGCACGCGGTGACCCATGACGTGGTTTTGGGCGGACACCTGGTGCCCAAGACGGGGAGTGTTAATTTCATGGTGGCGGATATAGGGTGGGACCCGCAAGTGTGGGAGGACCCCATGGCGTTCAAGCCGGAGAGGTTCTTGGGCAGCGGCGGAGAAGAAGAAGGGTTCGATTTGATGGGGAGTAGGGAGATTAAGATGATGCCGTTTGGGGCAGGGAGGAGGTCCTGTCCTGGGTCTGGATTGGCGGTGCTTCACCTGGAGTACTTTGTGGCGAATTTGGTGTGGAAATTCGAGTGGAGAGCTATGGACGGAGATGACGTGGACCTGTCTGAGAAGCAGGAGTTTACTATGGATGGAAAACTTGATGAAGGCGCTGCTAGTTTGATACTCGACATGAGGCCTTCAAAAGTCGTATCGACTGAATTGGTCTTCTTATTTATAGCTGTTGGATCAAATTTTAACGATTCGGATCCTGAACTTGATGAATTAGAAAAAAGAGATCCGGATCATTTTCCAATAGATAATTATCCAACAGCTTTGATGATCTCCGCATCATTCACCATGGAAACCTGGTTCCTTATCCTCATTGCCCTCTGCGTCTCCTTCCTCCTCAAACCCCTTCTTTCTCTATTCCTACCCACTTCCGTCTCCAAACCCAAGCTCCCTCCCGGTCCCCGCTCCATCCCCATCATCGGCGGCTTCTTGTGGATCCTGAAACCCTTCTTGGAGATCGAGCTTATCATCCGCAAACTCCAGGTCCAATACGGGCCCATCATCTCCTTCTCCATCGGCTCCCGCTCCACCGTCTTCATCGCCGACCGCTCCCTCGCCCACCAGGCCTTCATCCAGAACGGCGCCGTATTCGCTGACCGACCTGGGGCCTTGGTCACCAACAAGTACACTACCAGTAACCAGCACACAATCGCCTCCGCCGGATACGGCACTACGTGGCGCCTCCTCCGCCGCAACCTCACCTCCGAAATCCTCCACCCTTCCCGCGTCAAATCCTACGGCAACGCCCGCAAATGGGTTCTTGACATCCTCGTCAACCGCCTCAAAACCATGTCTCAATCCCACTCCGAAGGTGGCGTCGTCGGTAGTGTTAGGGTGGTCGACCACTTCCAATACGCCCTGTTCTGCCTCCTCGTTCTAATGTGCTTCGGCGACAAATTGAACGAAGAGCAAATCAAAGAAATCGAGCGGGTGCAGCGCCAACAACATTTGAGTTTCCGGCGGTTCAACATCCTCAATTTCAAGCCGAAATTGACGAAGATTTTCCTAAAAAATCGGTGGCGGGAATTCTTCAAAATCCTCGAGGAACAACGAGAAGTGCTCGTCCCTCTGATTCGAGCACGACAAAACAAGGCAAAGCAGGGCAGGAAAAACGACGACAAAGACGATGAATCGGTGTTGTCGTATACCGATACGCTGCTGGACCTCGAGCTTCCCGACGGCAAAGAGAAGCGGAAGCTTTCGGAGGACGAAATGGTCAACCTCTGCTCGGAGTTTCTCAACGCCGGCACCGACACTACTTCCACCGCGCTGCAGTGGGTCATGGCTAACATAGTAAAGTATCCGCAAGTTCAGGAGAAGCTGTTTTCAGAGATTAAACGGGTTATGGGAGAAACGAAGGAGGAGGTGCGAGAGGAGGTCCTGCACAAGCTGCCGTATCTGAAAGCCGTGATCTTGGAGGGGCTGAGGCGCCACCCACCGGGGCACGTTTTGTTTCCGCACTCGGTGACGCATGACGTGGTTTTGGGAGGACACCTGGTGCCCAAAAACGGGACAGTCAATGTCATGGTGGCGGATATAGGGTGGAACGCGCAAGTGTGGGAGGACCCCATGGCTTTCAAGCCGGAGAGGTTCTTGGGCAGCAGCGGCGGAGAAGAAGGGTTCGATTTGACGGGAAGCAGGGAGATTAAGATGATGCCGTTTGGGGTGGGGAGGAGGTCCTGTCCTGGGGCGGGGTTGGCGGTGCTTCATCTGGAGTACTTTGTGGCGAATTTGGTTTGGAAGTTTCAGTGGAGGGCTGTGGAGGGAGATGACGTTGACCTGTCAGAGAAGCAGGAGTTTACTGTGGTAATGAAGAATCCATTGCAAGCCCACGTAATCCCAAGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

1097

Amino Acids

124.43

Weight (kDa)

8.37

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 505 3.2e-87 Cytochrome P450
p450 PF00067 611 - 1087 9e-92 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 482, 2210, 2654
AbsI CCTCGAGG 2 cut(s) 752, 2480
AccB7I CCANNNNNTGG 1 cut(s) 1189
AccBSI CCGCTC 6 cut(s) 237, 261, 1845, 1965, 1989, 2368
AccI GTMKAC 3 cut(s) 552, 2280, 2585
AccII CGCG 6 cut(s) 438, 642, 1175, 2166, 2708, 2990
AccIII TCCGGA 1 cut(s) 1669
AclWI GGATC 8 cut(s) 998, 1624, 1631, 1644, 1661, 1680, 1870, 1883
AcoI YGGCCR 2 cut(s) 324, 996
AcsI RAATTY 7 cut(s) 842, 1441, 1454, 1621, 2465, 3169, 3285
AcyI GRCGYC 6 cut(s) 391, 1141, 2022, 2119, 2256, 2869
AdeI CACNNNGTG 6 cut(s) 1162, 1177, 1210, 2115, 2905, 2938
AfaI GTAC 6 cut(s) 338, 857, 1013, 1430, 2066, 3158
AjiI CACGTC 3 cut(s) 1189, 1486, 2917
AjuI GAANNNNNNNTTGG 2 cut(s) 1874, 1906
AleI CACNNNNGTG 1 cut(s) 985
AloI GAACNNNNNNTCC 3 cut(s) 28, 1724, 1756
Alw21I GWGCWC 5 cut(s) 171, 774, 793, 2502, 2521
Alw26I GTCTC 5 cut(s) 43, 510, 1771, 1819, 2238
AlwI GGATC 8 cut(s) 998, 1624, 1631, 1644, 1661, 1680, 1870, 1883
AlwNI CAGNNNCTG 1 cut(s) 936
Ama87I CYCGRG 6 cut(s) 170, 315, 752, 878, 2480, 2606
Aor13HI TCCGGA 1 cut(s) 1669
ApaI GGGCCC 3 cut(s) 114, 210, 1938
ApoI RAATTY 7 cut(s) 842, 1441, 1454, 1621, 2465, 3169, 3285
ArsI GACNNNNNNTTYG 6 cut(s) 827, 859, 1908, 1940, 2241, 2273
Asp700I GAANNNNTTC 2 cut(s) 1886, 2441
AsuC2I CCSGG 5 cut(s) 316, 317, 1152, 1836, 2880
AsuHPI GGTGA 8 cut(s) 16, 614, 1189, 1412, 1717, 2047, 2131, 2917
AvaI CYCGRG 6 cut(s) 170, 315, 752, 878, 2480, 2606
BaeGI GKGCMC 7 cut(s) 114, 210, 1158, 1215, 1938, 2886, 2943
BalI TGGCCA 2 cut(s) 326, 998
BamHI GGATCC 2 cut(s) 1636, 1875
BanII GRGCYC 4 cut(s) 114, 171, 210, 1938
BbsI GAAGAC 3 cut(s) 238, 1588, 1966
Bbv12I GWGCWC 5 cut(s) 171, 774, 793, 2502, 2521
BbvCI CCTCAGC 2 cut(s) 1136, 2864
BccI CCATC 9 cut(s) 92, 134, 218, 1336, 1514, 1856, 1862, 1946, 1961
BciVI GTATCC 2 cut(s) 2096, 2751
BcnI CCSGG 5 cut(s) 316, 317, 1152, 1836, 2880
BcoDI GTCTC 5 cut(s) 43, 510, 1771, 1819, 2238
BfaI CTAG 2 cut(s) 482, 1545
BfmI CTRYAG 3 cut(s) 191, 983, 2711
BfoI RGCGCY 8 cut(s) 394, 652, 1144, 1542, 2025, 2122, 2380, 2872
BfuI GTATCC 2 cut(s) 2096, 2751
BglI GCCNNNNNGGC 1 cut(s) 2003
BmcAI AGTACT 2 cut(s) 1430, 3158
BmeT110I CYCGRG 6 cut(s) 170, 315, 752, 878, 2480, 2606
BmgBI CACGTC 3 cut(s) 1189, 1486, 2917
BmrI ACTGGG 1 cut(s) 508
BmsI GCATC 3 cut(s) 1356, 1727, 3084
BmuI ACTGGG 1 cut(s) 508
BpiI GAAGAC 3 cut(s) 238, 1588, 1966
BplI GAGNNNNNCTC 4 cut(s) 756, 788, 2484, 2516
BpmI CTGGAG 3 cut(s) 1445, 1904, 3173
Bpu10I CCTNAGC 2 cut(s) 1136, 2864
BpuMI CCSGG 5 cut(s) 316, 317, 1152, 1836, 2880
BsaAI YACGTR 2 cut(s) 2115, 3274
BsaBI GATNNNNATC 3 cut(s) 1635, 1671, 1692
BsaHI GRCGYC 6 cut(s) 391, 1141, 2022, 2119, 2256, 2869
BsaWI WCCGGW 1 cut(s) 1669
Bse118I RCCGGY 2 cut(s) 956, 2684
Bse1I ACTGG 2 cut(s) 514, 2073
Bse3DI GCAATG 2 cut(s) 1752, 3260
Bse8I GATNNNNATC 3 cut(s) 1635, 1671, 1692
BseAI TCCGGA 1 cut(s) 1669
BseJI GATNNNNATC 3 cut(s) 1635, 1671, 1692
BseMI GCAATG 2 cut(s) 1752, 3260
BseMII CTCAG 4 cut(s) 954, 1127, 1488, 2855
BseNI ACTGG 2 cut(s) 514, 2073
BseSI GKGCMC 7 cut(s) 114, 210, 1158, 1215, 1938, 2886, 2943
BsgI GTGCAG 2 cut(s) 2393, 2807
Bsh1236I CGCG 6 cut(s) 438, 642, 1175, 2166, 2708, 2990
Bsh1285I CGRYCG 3 cut(s) 259, 310, 1987
BsiEI CGRYCG 3 cut(s) 259, 310, 1987
BsiHKAI GWGCWC 5 cut(s) 171, 774, 793, 2502, 2521
BsiHKCI CYCGRG 6 cut(s) 170, 315, 752, 878, 2480, 2606
BslFI GGGAC 5 cut(s) 761, 1271, 1824, 2489, 2965
BsmAI GTCTC 5 cut(s) 43, 510, 1771, 1819, 2238
BsmBI CGTCTC 3 cut(s) 43, 1771, 1819
BsmFI GGGAC 5 cut(s) 761, 1271, 1824, 2489, 2965
BsmI GAATGC 1 cut(s) 527
BsoBI CYCGRG 6 cut(s) 170, 315, 752, 878, 2480, 2606
Bsp120I GGGCCC 3 cut(s) 110, 206, 1934
Bsp13I TCCGGA 1 cut(s) 1669
Bsp19I CCATGG 3 cut(s) 1281, 1728, 3009
BspCNI CTCAG 4 cut(s) 953, 1128, 1489, 2856
BspEI TCCGGA 1 cut(s) 1669
BspFNI CGCG 6 cut(s) 438, 642, 1175, 2166, 2708, 2990
BspMAI CTGCAG 2 cut(s) 987, 2715
BspPI GGATC 8 cut(s) 998, 1624, 1631, 1644, 1661, 1680, 1870, 1883
BspQI GCTCTTC 2 cut(s) 612, 2340
BsrBI CCGCTC 6 cut(s) 237, 261, 1845, 1965, 1989, 2368
BsrDI GCAATG 2 cut(s) 1752, 3260
BsrFI RCCGGY 2 cut(s) 956, 2684
BsrI ACTGG 2 cut(s) 514, 2073
BssAI RCCGGY 2 cut(s) 956, 2684
BssNAI GTATAC 1 cut(s) 2586
BssNI GRCGYC 6 cut(s) 391, 1141, 2022, 2119, 2256, 2869
BssT1I CCWWGG 5 cut(s) 321, 1281, 1728, 2049, 3009
Bst1107I GTATAC 1 cut(s) 2586
Bst4CI ACNGT 4 cut(s) 293, 1972, 2956, 3244
Bst6I CTCTTC 2 cut(s) 612, 2340
BstACI GRCGYC 6 cut(s) 391, 1141, 2022, 2119, 2256, 2869
BstAPI GCANNNNNTGC 3 cut(s) 1102, 1162, 2830
BstBAI YACGTR 2 cut(s) 2115, 3274
BstC8I GCNNGC 6 cut(s) 460, 958, 1173, 2188, 2686, 3267
BstDEI CTNAG 4 cut(s) 940, 1136, 1497, 2864
BstDSI CCRYGG 3 cut(s) 1281, 1728, 3009
BstEII GGTNACC 2 cut(s) 1177, 2053
BstFNI CGCG 6 cut(s) 438, 642, 1175, 2166, 2708, 2990
BstH2I RGCGCY 8 cut(s) 394, 652, 1144, 1542, 2025, 2122, 2380, 2872
BstMAI GTCTC 5 cut(s) 43, 510, 1771, 1819, 2238
BstMCI CGRYCG 3 cut(s) 259, 310, 1987
BstPI GGTNACC 2 cut(s) 1177, 2053
BstSFI CTRYAG 3 cut(s) 191, 983, 2711
BstSLI GKGCMC 7 cut(s) 114, 210, 1158, 1215, 1938, 2886, 2943
BstUI CGCG 6 cut(s) 438, 642, 1175, 2166, 2708, 2990
BstV2I GAAGAC 3 cut(s) 238, 1588, 1966
BstX2I RGATCY 3 cut(s) 1636, 1666, 1875
BstYI RGATCY 3 cut(s) 1636, 1666, 1875
BstZ17I GTATAC 1 cut(s) 2586
BsuI GTATCC 2 cut(s) 2096, 2751
BtgI CCRYGG 3 cut(s) 1281, 1728, 3009
BtgZI GCGATG 1 cut(s) 1963
BtrI CACGTC 3 cut(s) 1189, 1486, 2917
BtsI GCAGTG 3 cut(s) 980, 992, 2720
BtsIMutI CAGTG 5 cut(s) 858, 980, 992, 2720, 3194
Cac8I GCNNGC 6 cut(s) 460, 958, 1173, 2188, 2686, 3267
CaiI CAGNNNCTG 1 cut(s) 936
Cfr10I RCCGGY 2 cut(s) 956, 2684
Cfr9I CCCGGG 1 cut(s) 315
CseI GACGC 6 cut(s) 25, 427, 1753, 2155, 2245, 2917
CsiI ACCWGGT 4 cut(s) 8, 1206, 1736, 2934
Csp6I GTAC 6 cut(s) 337, 856, 1012, 1429, 2065, 3157
CviQI GTAC 6 cut(s) 337, 856, 1012, 1429, 2065, 3157
DdeI CTNAG 4 cut(s) 940, 1136, 1497, 2864
DinI GGCGCC 5 cut(s) 392, 1142, 2023, 2120, 2870
DraIII CACNNNGTG 6 cut(s) 1162, 1177, 1210, 2115, 2905, 2938
DrdI GACNNNNNNGTC 3 cut(s) 482, 2210, 2654
DseDI GACNNNNNNGTC 3 cut(s) 482, 2210, 2654
EaeI YGGCCR 2 cut(s) 324, 996
Eam1104I CTCTTC 2 cut(s) 612, 2340
EarI CTCTTC 2 cut(s) 612, 2340
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 5 cut(s) 321, 1281, 1728, 2049, 3009
Eco147I AGGCCT 2 cut(s) 1567, 2006
Eco24I GRGCYC 4 cut(s) 114, 171, 210, 1938
Eco53kI GAGCTC 1 cut(s) 169
Eco88I CYCGRG 6 cut(s) 170, 315, 752, 878, 2480, 2606
Eco91I GGTNACC 2 cut(s) 1177, 2053
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 9 cut(s) 111, 1090, 1258, 1276, 1389, 2046, 2818, 3004, 3117
EcoO65I GGTNACC 2 cut(s) 1177, 2053
EcoRI GAATTC 1 cut(s) 2465
EcoT14I CCWWGG 5 cut(s) 321, 1281, 1728, 2049, 3009
EcoT38I GRGCYC 4 cut(s) 114, 171, 210, 1938
EgeI GGCGCC 5 cut(s) 392, 1142, 2023, 2120, 2870
EheI GGCGCC 5 cut(s) 392, 1142, 2023, 2120, 2870
ErhI CCWWGG 5 cut(s) 321, 1281, 1728, 2049, 3009
Esp3I CGTCTC 3 cut(s) 43, 1771, 1819
FalI AAGNNNNNCTT 4 cut(s) 45, 77, 701, 733
FaqI GGGAC 5 cut(s) 761, 1271, 1824, 2489, 2965
FblI GTMKAC 3 cut(s) 552, 2280, 2585
FriOI GRGCYC 4 cut(s) 114, 171, 210, 1938
FspBI CTAG 2 cut(s) 482, 1545
GsuI CTGGAG 3 cut(s) 1445, 1904, 3173
HaeII RGCGCY 8 cut(s) 394, 652, 1144, 1542, 2025, 2122, 2380, 2872
HgaI GACGC 6 cut(s) 25, 427, 1753, 2155, 2245, 2917
Hin1I GRCGYC 6 cut(s) 391, 1141, 2022, 2119, 2256, 2869
HincII GTYRAC 6 cut(s) 487, 553, 2215, 2281, 2659, 3217
HindII GTYRAC 6 cut(s) 487, 553, 2215, 2281, 2659, 3217
HindIII AAGCTT 2 cut(s) 908, 2636
HinfI GANTC 7 cut(s) 503, 712, 784, 1631, 2512, 2570, 3256
HphI GGTGA 8 cut(s) 16, 614, 1189, 1412, 1717, 2047, 2131, 2917
Hpy99I CGWCG 5 cut(s) 893, 2261, 2264, 2558, 2621
HpyCH4III ACNGT 4 cut(s) 293, 1972, 2956, 3244
HpyCH4IV ACGT 9 cut(s) 339, 1002, 1188, 1485, 2114, 2886, 2916, 3213, 3273
HpyCH4V TGCA 6 cut(s) 985, 1096, 2374, 2713, 2824, 3265
HpyF3I CTNAG 4 cut(s) 940, 1136, 1497, 2864
HpySE526I ACGT 9 cut(s) 339, 1002, 1188, 1485, 2114, 2886, 2916, 3213, 3273
Hsp92I GRCGYC 6 cut(s) 391, 1141, 2022, 2119, 2256, 2869
KasI GGCGCC 5 cut(s) 390, 1140, 2021, 2118, 2868
KflI GGGWCCC 1 cut(s) 1258
Kpn2I TCCGGA 1 cut(s) 1669
KroI GCCGGC 2 cut(s) 956, 2684
KroNI GCCGGC 2 cut(s) 958, 2686
LguI GCTCTTC 2 cut(s) 612, 2340
LweI GCATC 3 cut(s) 1356, 1727, 3084
MabI ACCWGGT 4 cut(s) 8, 1206, 1736, 2934
MaeI CTAG 2 cut(s) 482, 1545
MaeII ACGT 9 cut(s) 339, 1002, 1188, 1485, 2114, 2886, 2916, 3213, 3273
MaeIII GTNAC 5 cut(s) 602, 1177, 2053, 2075, 2905
MbiI CCGCTC 6 cut(s) 237, 261, 1845, 1965, 1989, 2368
MflI RGATCY 3 cut(s) 1636, 1666, 1875
MlsI TGGCCA 2 cut(s) 326, 998
MluNI TGGCCA 2 cut(s) 326, 998
Mly113I GGCGCC 5 cut(s) 391, 1141, 2022, 2119, 2869
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 2 cut(s) 1594, 1721
Mox20I TGGCCA 2 cut(s) 326, 998
MroI TCCGGA 1 cut(s) 1669
MroNI GCCGGC 2 cut(s) 956, 2684
MroXI GAANNNNTTC 2 cut(s) 1886, 2441
MscI TGGCCA 2 cut(s) 326, 998
MseI TTAA 8 cut(s) 281, 719, 1047, 1230, 1359, 1626, 2775, 3087
MslI CAYNNNNRTG 1 cut(s) 985
Msp20I TGGCCA 2 cut(s) 326, 998
MspA1I CMGCKG 2 cut(s) 1314, 3045
Mva1269I GAATGC 1 cut(s) 527
MvnI CGCG 6 cut(s) 438, 642, 1175, 2166, 2708, 2990
NaeI GCCGGC 2 cut(s) 958, 2686
NarI GGCGCC 5 cut(s) 391, 1141, 2022, 2119, 2869
NciI CCSGG 5 cut(s) 316, 317, 1152, 1836, 2880
NcoI CCATGG 3 cut(s) 1281, 1728, 3009
NgoMIV GCCGGC 2 cut(s) 956, 2684
NmuCI GTSAC 4 cut(s) 602, 1177, 2053, 2905
OliI CACNNNNGTG 1 cut(s) 985
PaeR7I CTCGAG 5 cut(s) 170, 752, 878, 2480, 2606
PceI AGGCCT 2 cut(s) 1567, 2006
PciSI GCTCTTC 2 cut(s) 612, 2340
PcsI WCGNNNNNNNCGW 7 cut(s) 885, 960, 1482, 2562, 2579, 2613, 2688
PctI GAATGC 1 cut(s) 527
PdiI GCCGGC 2 cut(s) 958, 2686
PdmI GAANNNNTTC 2 cut(s) 1886, 2441
PfeI GAWTC 6 cut(s) 712, 784, 1631, 2512, 2570, 3256
PflMI CCANNNNNTGG 1 cut(s) 1189
PleI GAGTC 1 cut(s) 511
PluTI GGCGCC 5 cut(s) 394, 1144, 2025, 2122, 2872
PpsI GAGTC 1 cut(s) 511
Ppu21I YACGTR 2 cut(s) 2115, 3274
PpuMI RGGWCCY 7 cut(s) 1090, 1258, 1276, 1389, 2818, 3004, 3117
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 7 cut(s) 1090, 1258, 1276, 1389, 2818, 3004, 3117
PspEI GGTNACC 2 cut(s) 1177, 2053
PspOMI GGGCCC 3 cut(s) 110, 206, 1934
PspPPI RGGWCCY 7 cut(s) 1090, 1258, 1276, 1389, 2818, 3004, 3117
PspXI VCTCGAGB 5 cut(s) 170, 752, 878, 2480, 2606
PstI CTGCAG 2 cut(s) 987, 2715
PstNI CAGNNNCTG 1 cut(s) 936
PsuI RGATCY 3 cut(s) 1636, 1666, 1875
RsaI GTAC 6 cut(s) 338, 857, 1013, 1430, 2066, 3158
RsaNI GTAC 6 cut(s) 337, 856, 1012, 1429, 2065, 3157
RseI CAYNNNNRTG 1 cut(s) 985
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 2 cut(s) 551, 2279
SapI GCTCTTC 2 cut(s) 612, 2340
SaqAI TTAA 8 cut(s) 281, 719, 1047, 1230, 1359, 1626, 2775, 3087
ScaI AGTACT 2 cut(s) 1430, 3158
SchI GAGTC 1 cut(s) 512
SexAI ACCWGGT 4 cut(s) 8, 1206, 1736, 2934
SfaNI GCATC 3 cut(s) 1356, 1727, 3084
SfcI CTRYAG 3 cut(s) 191, 983, 2711
SfoI GGCGCC 5 cut(s) 392, 1142, 2023, 2120, 2870
Sfr274I CTCGAG 5 cut(s) 170, 752, 878, 2480, 2606
SlaI CTCGAG 5 cut(s) 170, 752, 878, 2480, 2606
SmaI CCCGGG 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 985
SmlI CTYRAG 5 cut(s) 170, 752, 878, 2480, 2606
SmoI CTYRAG 5 cut(s) 170, 752, 878, 2480, 2606
SseBI AGGCCT 2 cut(s) 1567, 2006
SspDI GGCGCC 5 cut(s) 390, 1140, 2021, 2118, 2868
SspMI CTAG 2 cut(s) 482, 1545
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 2 cut(s) 1567, 2006
StyI CCWWGG 5 cut(s) 321, 1281, 1728, 2049, 3009
TaaI ACNGT 4 cut(s) 293, 1972, 2956, 3244
TaiI ACGT 9 cut(s) 342, 1005, 1191, 1488, 2117, 2889, 2919, 3216, 3276
TaqII GACCGA 1 cut(s) 2052
TatI WGTACW 3 cut(s) 1428, 2064, 3156
TfiI GAWTC 6 cut(s) 712, 784, 1631, 2512, 2570, 3256
Tru1I TTAA 8 cut(s) 281, 719, 1047, 1230, 1359, 1626, 2775, 3087
Tru9I TTAA 8 cut(s) 281, 719, 1047, 1230, 1359, 1626, 2775, 3087
TscAI CASTG 5 cut(s) 865, 987, 992, 2720, 3194
TseFI GTSAC 4 cut(s) 602, 1177, 2053, 2905
Tsp45I GTSAC 4 cut(s) 602, 1177, 2053, 2905
TspGWI ACGGA 3 cut(s) 1082, 1491, 1801
TspMI CCCGGG 1 cut(s) 315
TspRI CASTG 5 cut(s) 865, 987, 992, 2720, 3194
Van91I CCANNNNNTGG 1 cut(s) 1189
XapI RAATTY 7 cut(s) 842, 1441, 1454, 1621, 2465, 3169, 3285
XcmI CCANNNNNNNNNTGG 1 cut(s) 2249
XhoI CTCGAG 5 cut(s) 170, 752, 878, 2480, 2606
XmaI CCCGGG 1 cut(s) 315
XmiI GTMKAC 3 cut(s) 552, 2280, 2585
XmnI GAANNNNTTC 2 cut(s) 1886, 2441
XspI CTAG 2 cut(s) 482, 1545
ZrmI AGTACT 2 cut(s) 1430, 3158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.