Rh7DG340900

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000853
Physical Location & Seq
Reverse (-)
44933122 .. 44933511
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG340900.1

Sequence Viewer

Length: 390 bp
ATGGTAAACAAGAGCGATGATTATGTACTATGTTACCTAGACACGCTCTGGGAACTCGAGCTTCCGGACGAGAAGAGGAAGCTGAACAAAAAAGATATCATTAGCCTCTATTCGGAGTTTCTCAGTGCAGGAACGGATACTACTTCCACCGCGCTGCAGTGGATCATGGCCAATATCGTCAAGTATCAAACTATCCAGGAGAGGCTTCTCGCTGAGATTAAAGGAGTTGTGGAGGAAACAGAGGAGGTGGTGAAGGAAGAGGACTTGCAGAAGATGCCTTATTTAAAAGCGGTGATCTTGGAGGGTTTAAGGCGCCACCCACCTGGCCACTTTGCGCTGCCGCACTCTGTCACGCACGACATCGTTTTGGATGGTTATGTGGTGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

129

Amino Acids

14.83

Weight (kDa)

4.93

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 23 - 129 6.1e-32 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 312, 382
AccII CGCG 1 cut(s) 152
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 3 cut(s) 150, 290, 341
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 2 cut(s) 168, 325
AcyI GRCGYC 1 cut(s) 313
AfaI GTAC 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 112
AjnI CCWGG 2 cut(s) 195, 322
AluBI AGCT 2 cut(s) 61, 82
AluI AGCT 2 cut(s) 61, 82
AlwI GGATC 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 64
AoxI GGCC 2 cut(s) 168, 325
ApeKI GCWGC 2 cut(s) 154, 337
AspLEI GCGC 3 cut(s) 154, 315, 337
AsuHPI GGTGA 2 cut(s) 262, 304
AvaI CYCGRG 1 cut(s) 56
BalI TGGCCA 2 cut(s) 170, 327
BanI GGYRCC 2 cut(s) 312, 382
BbvI GCAGC 2 cut(s) 141, 324
BccI CCATC 1 cut(s) 365
BceAI ACGGC 1 cut(s) 370
BciT130I CCWGG 2 cut(s) 197, 324
BciVI GTATCC 1 cut(s) 130
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 155
BfoI RGCGCY 1 cut(s) 316
BfuI GTATCC 1 cut(s) 130
BisI GCNGC 3 cut(s) 155, 338, 341
BlsI GCNGC 3 cut(s) 156, 339, 342
Bme1390I CCNGG 2 cut(s) 197, 324
BmeT110I CYCGRG 1 cut(s) 56
BmiI GGNNCC 2 cut(s) 314, 384
BmrFI CCNGG 2 cut(s) 197, 324
BmsI GCATC 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 313
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 112
BseAI TCCGGA 1 cut(s) 64
BseBI CCWGG 2 cut(s) 197, 324
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMII CTCAG 2 cut(s) 136, 204
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 141, 324
BsgI GTGCAG 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 2 cut(s) 170, 327
BshNI GGYRCC 2 cut(s) 312, 382
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 1 cut(s) 112
BsnI GGCC 2 cut(s) 170, 327
BsoBI CYCGRG 1 cut(s) 56
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 2 cut(s) 162, 294
BspACI CCGC 3 cut(s) 150, 290, 341
BspANI GGCC 2 cut(s) 170, 327
BspCNI CTCAG 2 cut(s) 135, 205
BspEI TCCGGA 1 cut(s) 64
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 2 cut(s) 314, 384
BspMAI CTGCAG 1 cut(s) 159
BspPI GGATC 1 cut(s) 170
BspT107I GGYRCC 2 cut(s) 312, 382
BssMI GATC 2 cut(s) 162, 294
BssNI GRCGYC 1 cut(s) 313
Bst2UI CCWGG 2 cut(s) 197, 324
Bst6I CTCTTC 2 cut(s) 68, 252
BstACI GRCGYC 1 cut(s) 313
BstAPI GCANNNNNTGC 1 cut(s) 274
BstDEI CTNAG 2 cut(s) 122, 213
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 3 cut(s) 154, 315, 337
BstKTI GATC 2 cut(s) 165, 297
BstMBI GATC 2 cut(s) 162, 294
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNI CCWGG 2 cut(s) 197, 324
BstSCI CCNGG 2 cut(s) 195, 322
BstSFI CTRYAG 1 cut(s) 155
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 2 cut(s) 141, 324
BstXI CCANNNNNNTGG 1 cut(s) 323
BsuI GTATCC 1 cut(s) 130
BsuRI GGCC 2 cut(s) 170, 327
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 2 cut(s) 130, 164
CfoI GCGC 3 cut(s) 154, 315, 337
Csp6I GTAC 1 cut(s) 26
CviAII CATG 1 cut(s) 166
CviJI RGCY 6 cut(s) 61, 82, 105, 170, 205, 327
CviKI_1 RGCY 6 cut(s) 61, 82, 105, 170, 205, 327
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 2 cut(s) 122, 213
DinI GGCGCC 1 cut(s) 314
DpnI GATC 2 cut(s) 164, 296
DpnII GATC 2 cut(s) 162, 294
DraI TTTAAA 1 cut(s) 285
EaeI YGGCCR 2 cut(s) 168, 325
Eam1104I CTCTTC 2 cut(s) 68, 252
EarI CTCTTC 2 cut(s) 68, 252
Eco32I GATATC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 56
EcoRII CCWGG 2 cut(s) 195, 322
EcoRV GATATC 1 cut(s) 97
EgeI GGCGCC 1 cut(s) 314
EheI GGCGCC 1 cut(s) 314
FaeI CATG 1 cut(s) 169
FaiI YATR 4 cut(s) 24, 31, 167, 378
FatI CATG 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 155, 338, 341
FokI GGATG 1 cut(s) 383
Fsp4HI GCNGC 3 cut(s) 155, 338, 341
FspBI CTAG 1 cut(s) 38
GlaI GCGC 3 cut(s) 153, 314, 336
GluI GCNGC 3 cut(s) 155, 338, 341
HaeII RGCGCY 1 cut(s) 316
HaeIII GGCC 2 cut(s) 170, 327
HapII CCGG 1 cut(s) 65
HhaI GCGC 3 cut(s) 154, 315, 337
Hin1I GRCGYC 1 cut(s) 313
Hin1II CATG 1 cut(s) 169
Hin6I GCGC 3 cut(s) 152, 313, 335
HinP1I GCGC 3 cut(s) 152, 313, 335
HpaII CCGG 1 cut(s) 65
HphI GGTGA 2 cut(s) 262, 304
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 1 cut(s) 65
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 247
HpyCH4V TGCA 3 cut(s) 128, 157, 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpyF3I CTNAG 2 cut(s) 122, 213
Hsp92I GRCGYC 1 cut(s) 313
Hsp92II CATG 1 cut(s) 169
HspAI GCGC 3 cut(s) 152, 313, 335
KasI GGCGCC 1 cut(s) 312
Kpn2I TCCGGA 1 cut(s) 64
Kzo9I GATC 2 cut(s) 162, 294
LpnPI CCDG 7 cut(s) 34, 78, 114, 182, 209, 309, 336
Lsp1109I GCAGC 2 cut(s) 141, 324
LweI GCATC 1 cut(s) 264
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 2 cut(s) 32, 349
MalI GATC 2 cut(s) 164, 296
MboI GATC 2 cut(s) 162, 294
MboII GAAGA 3 cut(s) 85, 269, 283
MlsI TGGCCA 2 cut(s) 170, 327
MluNI TGGCCA 2 cut(s) 170, 327
Mly113I GGCGCC 1 cut(s) 313
MnlI CCTC 8 cut(s) 69, 116, 195, 226, 235, 238, 253, 295
Mox20I TGGCCA 2 cut(s) 170, 327
MroI TCCGGA 1 cut(s) 64
MscI TGGCCA 2 cut(s) 170, 327
MseI TTAA 3 cut(s) 219, 284, 308
Msp20I TGGCCA 2 cut(s) 170, 327
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 197, 324
MvaI CCWGG 2 cut(s) 197, 324
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 274
NarI GGCGCC 1 cut(s) 313
NdeII GATC 2 cut(s) 162, 294
NlaIII CATG 1 cut(s) 169
NlaIV GGNNCC 2 cut(s) 314, 384
NmuCI GTSAC 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 56
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 3 cut(s) 156, 339, 342
PluTI GGCGCC 1 cut(s) 316
Psp6I CCWGG 2 cut(s) 195, 322
PspGI CCWGG 2 cut(s) 195, 322
PspN4I GGNNCC 2 cut(s) 314, 384
PspXI VCTCGAGB 1 cut(s) 56
PsrI GAACNNNNNNTAC 2 cut(s) 124, 156
PstI CTGCAG 1 cut(s) 159
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
SaqAI TTAA 3 cut(s) 219, 284, 308
SatI GCNGC 3 cut(s) 155, 338, 341
Sau3AI GATC 2 cut(s) 162, 294
ScrFI CCNGG 2 cut(s) 197, 324
SetI ASST 5 cut(s) 39, 63, 84, 249, 325
SfaNI GCATC 1 cut(s) 264
SfcI CTRYAG 1 cut(s) 155
SfoI GGCGCC 1 cut(s) 314
Sfr274I CTCGAG 1 cut(s) 56
SlaI CTCGAG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SsiI CCGC 3 cut(s) 150, 290, 341
SspDI GGCGCC 1 cut(s) 312
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 195, 322
TaqI TCGA 1 cut(s) 57
TatI WGTACW 1 cut(s) 25
TauI GCSGC 1 cut(s) 343
Tru1I TTAA 3 cut(s) 219, 284, 308
Tru9I TTAA 3 cut(s) 219, 284, 308
TscAI CASTG 2 cut(s) 130, 164
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 2 cut(s) 154, 337
Tsp45I GTSAC 1 cut(s) 349
TspGWI ACGGA 1 cut(s) 149
TspRI CASTG 2 cut(s) 130, 164
XhoI CTCGAG 1 cut(s) 56
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.