MD01G1204400.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000853
Physical Location & Seq
Forward (+)
29984108 .. 29989047
4940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1204400.v1.1.491

Sequence Viewer

Length: 3087 bp
ATGGAAACCTGGTTCCTGATCCTCATCGCCTTCTGCGTCTCCTTCCTCCTCAAACCCCTTCTTTCCCTATTCCTACCCACTTCCATCTCCAAACCCAAGCTCCCTCCTGGGCCCCGCGTCTTCCCCGTCATCGGCGGCTTCTTATTCCTCCTCAAATCCTTCTCGGAGCTCGAGCCTATCATCCGCAAACTCAAGGCCAAACACGGGCCCATTATCTCCCTCCGCATCGGCTCCCGCCCGGCCGTCTTCATCGCCGACCGCTCCCTCGCCCACCAGGCCTTAATCCAGAACGGCGCCGTGTTCGCCGACCGCCCCCGGGCCTCGGCCACCAACAAGTACTCCAGCAGCAACCAGCACAACATCAGCTCCGCCATCTACGGCCCCACTTGGCGCCTCCTCCGCCGCAACCTCACCTCCGAAATCCTTCACCCTTCCCGGGTCAAATCCTACGGCAACGCCCGCAAATGGGTTCTCGACATCCTCGTCAACCGCCTCAAAACCAAGTCTCAATCCCACTCCGAAGGCGCCAGCGCCGGCGTCAGGGTGGTCGACCACTTCCAATACGCCATGTTCTGCCTCCTGGTTCTGATGTGCTTCGGTGACAAATTGAACGAAGAGCAAATCAAAGAAATCGAGCGGGTGCAGCGCCAATTTCTTTTGAGTTCCGGGCGGTTCAATATCCTCAATTTCACCCCTAAATTGACGAAGATTATCTTCAAAAATCGGTGGCGGCAATTCTTCAAAATCCTCGAGGAACGACGAGAAGTGCTCGTCCCTCTGATTCGAGCAAGACAAAACAAGGCAAAGCAGGGCAGCAAAAACGACGACAATGACGATGAATTTGTGTTGTCGTATACTGATACGCTGCTGGACCTCGAGCTTCCCGACGGAAACAAAAAGCGGAAGCTTTCGGAGGGAGAAATGGTCAGCCTCTGCTCAGAGTTTCTGAACGGCGGCACCGACACGACTTCCACCGCGCTGCAGTGGATCATGGCCAACGTAGTGAAGTACCCGCAAGTTCAGGAGAAGCTGTTTGCGGAGATTAAAGGGGTTATGGGAGAAACGGAGGACGAGCTGCGGGAGGAGGTCCTGCACAAGCTGCCGTATCTGAAAGCCGTGATCTTGGAGGGGCTGAGGCGCCACCCGCCGGGCCACTTTGTGCTGCCGCACGCGGTGACGCATGACGTGGTTTTGGGCGGACACGTGGTGCCCAAGAACGGGAGTGTTAATTTCATGGTGGCGGATATAGGCTGGGACCCTCAAGTGTGGGAAGATCCCATGGCGTTCAAGCCGGAGAGGTTCTTGAGCAGCGGCGGAGGAGAAGAAGGGTTCGATTTGACGGGGAGCAGGGAGATTAAGATGATGCCGTTTGGGGCAGGGAGGAGAATCTGTCCTGCGTCGGGATTGGCGGTGCTTCACCTGGAGTACTTTGTGGCGAATTTGGTGTGGAAATTTGAGTGGAGAGCTGTGGAGGGAGATGACGTGGACCTGTCGGAGAAGCAGGAGTCTACTGTGGTGATGAAGAATCCATTGCAAGCCCACGGTGGTGGTTCTGGCACCCAACAAATTGTGATTGAAGGCCCCTTATTTGCCCTTTTGTCTGATTGCTTACGATTCACCATGGAAACCTGGTTCCTTGTCCTCATCGCCCTCTGCGTCTCCTTCCTCCTCAAACCCCTTCTCTCTCTATTCCTACCCACTTCCATCTCCAGACCCAATCTCCCTCTCGGGCCTCGCTTCTTCCCCATCATCGAGGGCTTCTTATTCCTCCTCAAATCTGCCTCGGAGCTCGAGCCTATTATCCGCAAACTCCAGGCCGAATACGGGCCCATCATCTCCCTCCGCATCGGCTCCCGCTCTACCGTCTTCGTCGCCGACCGCTCCCTCGCCCACCAGGCCTTAATCCAGAACGGAGCCGTGTTCGCCGACCGCCCCCGGGCCTTGGCCACCAACAAGTGTCTCAACAGCAACCAGCACACCATCGGCTCCGCCGGCTACGGTCCCTCGTGGCGTCTCCTCCGCCGCAATATCACCTCCGAAATCCTCCACCCTTCCCGCGTTAAATCCTACGGCAACGCCCGCAAATGGGTTCTCGACATCCTTGTCAACCGCCTCAAAACCGAGTCTCAATCCCACTCAGAAGACGTCGCCAGCGGCGTCAGGGTGGTCGACCATTTCCGATACGCCATGTTCTGCCTCCTGGTTCTGATGTGCTTCGGCGACAAAATGAACGAAGAGCAAATCAAAGAAATCGAGCGTCGGCAATTCTTCAAAATCCTCGAGGAACGACGAGAAGTGCTCGTCCGTCTGATTCGAGCACGACAAACCAGGGCGAAGCAGGGCAGCAAAACCGACGACAAAGACGATGAATTTGTGTTGTCGTATACTGATACGCTGCTGGACCTCGAACTTCCCGACGGCAACGAAAAGCGGAAGCTTTCGGAGGGAGAAATGGTCAGCCTCTGCTCAGAGTTTCTGAACGCCGGCACCGACACGACTTCCACCGCGCTGCAGTGGATCATGGCCAACGTAGTGAAGTACCCGCAAGTTCAGGAGAAGCTGTTTGCTGAGATTAAAGGGGTGATGGGAGAAACGGAGGAGGAGGTGCGGGAGGAGGTCCTACACAAGCTGCCGTATCTGAAAGCCGTGATCTTGGAGGGGCTGAGGCGCCACCCGCCGGCGCACTTTGTGCTGCCGCACGCGGTGACGCAAGACGTGGTTTTGGGCGGACACGTGGTGCCCAAGAACGGGAGTGTTAATTTCATGGTGGCGGATATAGGCTGGGACCCGCAAGTGTGGGAGGATCCCATGGCGTTCAAGCCCGAGAGGTTCTTGGGCAGCGGCGGAGAAGAAGGGTTCGATTTGACGGGGAGCAGGGAGATTAAGATGATGCCGTTTGGGGCAGGGAGGAGGATCTGTCCTGCGTCGGGATTGGCGGTGCTTCACCTGGAGTACTTTGTGGCGAATTTGGTTTGGAAGTTTCAGTGGAGGGCTGTGGAGGGAGATGACGTTGACCTGTCAGAGAAGCGGGAGTTTACTATGGTGATGAAGAATCCATTGCAAGCCCACGTAATCCCTAGAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

1029

Amino Acids

116.16

Weight (kDa)

8.72

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 510 5.5e-89 Cytochrome P450
p450 PF00067 575 - 758 5e-18 Cytochrome P450
p450 PF00067 754 - 1020 2.7e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 482, 2102
AatII GACGTC 1 cut(s) 2148
AbsI CCTCGAGG 2 cut(s) 749, 2279
AccBSI CCGCTC 4 cut(s) 261, 637, 1857, 1881
AccI GTMKAC 5 cut(s) 549, 854, 1508, 2169, 2384
AccII CGCG 6 cut(s) 117, 977, 1172, 2058, 2507, 2702
AclWI GGATC 7 cut(s) 13, 995, 1268, 2525, 2798, 2811, 2921
AcoI YGGCCR 5 cut(s) 240, 324, 993, 1944, 2523
AcsI RAATTY 6 cut(s) 839, 1438, 1451, 2369, 2965, 3081
AcvI CACGTG 2 cut(s) 1204, 2734
AcyI GRCGYC 9 cut(s) 294, 391, 525, 537, 1138, 2011, 2145, 2157, 2668
AdeI CACNNNGTG 6 cut(s) 1159, 1174, 1207, 2689, 2704, 2737
AfaI GTAC 5 cut(s) 338, 1010, 1427, 2540, 2954
AflIII ACRYGT 2 cut(s) 1201, 2731
AgsI TTSAA 8 cut(s) 610, 676, 718, 742, 1288, 1577, 2272, 2818
AjiI CACGTC 3 cut(s) 1186, 1483, 2716
AleI CACNNNNGTG 1 cut(s) 1545
AloI GAACNNNNNNTCC 3 cut(s) 28, 1616, 1648
Alw21I GWGCWC 5 cut(s) 171, 771, 1791, 2301, 2320
Alw26I GTCTC 6 cut(s) 43, 510, 1663, 1964, 2018, 2130
AlwI GGATC 7 cut(s) 13, 995, 1268, 2525, 2798, 2811, 2921
AlwNI CAGNNNCTG 2 cut(s) 933, 2463
ApaI GGGCCC 3 cut(s) 114, 210, 1830
ApoI RAATTY 6 cut(s) 839, 1438, 1451, 2369, 2965, 3081
ArsI GACNNNNNNTTYG 4 cut(s) 824, 856, 2354, 2386
Asp700I GAANNNNTTC 1 cut(s) 423
AsuC2I CCSGG 9 cut(s) 239, 316, 317, 436, 437, 667, 1149, 1936, 1937
AvaII GGWCC 8 cut(s) 871, 1087, 1255, 1486, 2000, 2401, 2617, 2785
BaeGI GKGCMC 5 cut(s) 114, 210, 1212, 1830, 2742
BalI TGGCCA 3 cut(s) 995, 1946, 2525
BamHI GGATCC 1 cut(s) 2803
BanII GRGCYC 5 cut(s) 114, 171, 210, 1791, 1830
BauI CACGAG 1 cut(s) 2005
BbrPI CACGTG 2 cut(s) 1204, 2734
BbsI GAAGAC 4 cut(s) 112, 238, 1858, 2148
Bbv12I GWGCWC 5 cut(s) 171, 771, 1791, 2301, 2320
BbvCI CCTCAGC 2 cut(s) 1133, 2663
BccI CCATC 7 cut(s) 92, 380, 1712, 1754, 1838, 1988, 2578
BcnI CCSGG 9 cut(s) 239, 316, 317, 436, 437, 667, 1149, 1936, 1937
BcoDI GTCTC 6 cut(s) 43, 510, 1663, 1964, 2018, 2130
BfaI CTAG 1 cut(s) 3078
BfmI CTRYAG 2 cut(s) 980, 2510
BfoI RGCGCY 7 cut(s) 297, 394, 528, 534, 649, 1141, 2671
BglI GCCNNNNNGGC 2 cut(s) 275, 1895
BmcAI AGTACT 3 cut(s) 338, 1427, 2954
Bme18I GGWCC 8 cut(s) 871, 1087, 1255, 1486, 2000, 2401, 2617, 2785
BmgBI CACGTC 3 cut(s) 1186, 1483, 2716
BmsI GCATC 4 cut(s) 234, 1353, 1854, 2880
BpiI GAAGAC 4 cut(s) 112, 238, 1858, 2148
BplI GAGNNNNNCTC 4 cut(s) 753, 785, 2283, 2315
BpmI CTGGAG 5 cut(s) 325, 1442, 1693, 1796, 2969
Bpu10I CCTNAGC 2 cut(s) 1133, 2663
BpuEI CTTGAG 3 cut(s) 176, 1245, 1324
BpuMI CCSGG 9 cut(s) 239, 316, 317, 436, 437, 667, 1149, 1936, 1937
BsaAI YACGTR 3 cut(s) 1204, 2734, 3070
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 9 cut(s) 294, 391, 525, 537, 1138, 2011, 2145, 2157, 2668
Bse118I RCCGGY 4 cut(s) 533, 1991, 2483, 2677
Bse3DI GCAATG 2 cut(s) 1529, 3056
Bse8I GATNNNNATC 1 cut(s) 23
BseGI GGATG 3 cut(s) 180, 477, 2097
BseJI GATNNNNATC 1 cut(s) 23
BseMI GCAATG 2 cut(s) 1529, 3056
BseMII CTCAG 6 cut(s) 951, 1124, 2151, 2481, 2559, 2654
BseSI GKGCMC 5 cut(s) 114, 210, 1212, 1830, 2742
BseX3I CGGCCG 1 cut(s) 240
BseYI CCCAGC 2 cut(s) 1251, 2781
BsgI GTGCAG 2 cut(s) 662, 1076
Bsh1236I CGCG 6 cut(s) 117, 977, 1172, 2058, 2507, 2702
Bsh1285I CGRYCG 5 cut(s) 243, 259, 310, 1879, 1930
BsiEI CGRYCG 5 cut(s) 243, 259, 310, 1879, 1930
BsiHKAI GWGCWC 5 cut(s) 171, 771, 1791, 2301, 2320
BslFI GGGAC 4 cut(s) 758, 1268, 1986, 2798
BsmAI GTCTC 6 cut(s) 43, 510, 1663, 1964, 2018, 2130
BsmBI CGTCTC 3 cut(s) 43, 1663, 2018
BsmFI GGGAC 4 cut(s) 758, 1268, 1986, 2798
Bsp120I GGGCCC 3 cut(s) 110, 206, 1826
Bsp143I GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
Bsp19I CCATGG 3 cut(s) 1278, 1620, 2808
BspCNI CTCAG 6 cut(s) 950, 1125, 2150, 2480, 2560, 2655
BspFNI CGCG 6 cut(s) 117, 977, 1172, 2058, 2507, 2702
BspMAI CTGCAG 2 cut(s) 984, 2514
BspPI GGATC 7 cut(s) 13, 995, 1268, 2525, 2798, 2811, 2921
BspQI GCTCTTC 2 cut(s) 609, 2229
BsrBI CCGCTC 4 cut(s) 261, 637, 1857, 1881
BsrDI GCAATG 2 cut(s) 1529, 3056
BsrFI RCCGGY 4 cut(s) 533, 1991, 2483, 2677
BssAI RCCGGY 4 cut(s) 533, 1991, 2483, 2677
BssMI GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
BssNAI GTATAC 2 cut(s) 855, 2385
BssNI GRCGYC 9 cut(s) 294, 391, 525, 537, 1138, 2011, 2145, 2157, 2668
BssSI CACGAG 1 cut(s) 2005
BssT1I CCWWGG 4 cut(s) 1278, 1620, 1941, 2808
Bst1107I GTATAC 2 cut(s) 855, 2385
Bst2BI CACGAG 1 cut(s) 2005
Bst4CI ACNGT 4 cut(s) 1513, 1544, 1864, 2000
Bst6I CTCTTC 2 cut(s) 609, 2229
BstACI GRCGYC 9 cut(s) 294, 391, 525, 537, 1138, 2011, 2145, 2157, 2668
BstAPI GCANNNNNTGC 2 cut(s) 1099, 2689
BstBAI YACGTR 3 cut(s) 1204, 2734, 3070
BstDEI CTNAG 6 cut(s) 937, 1133, 2137, 2467, 2568, 2663
BstDSI CCRYGG 4 cut(s) 1278, 1540, 1620, 2808
BstF5I GGATG 3 cut(s) 180, 477, 2097
BstFNI CGCG 6 cut(s) 117, 977, 1172, 2058, 2507, 2702
BstH2I RGCGCY 7 cut(s) 297, 394, 528, 534, 649, 1141, 2671
BstKTI GATC 8 cut(s) 21, 990, 1122, 1276, 2520, 2652, 2806, 2916
BstMAI GTCTC 6 cut(s) 43, 510, 1663, 1964, 2018, 2130
BstMBI GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
BstMCI CGRYCG 5 cut(s) 243, 259, 310, 1879, 1930
BstSFI CTRYAG 2 cut(s) 980, 2510
BstSLI GKGCMC 5 cut(s) 114, 210, 1212, 1830, 2742
BstUI CGCG 6 cut(s) 117, 977, 1172, 2058, 2507, 2702
BstV2I GAAGAC 4 cut(s) 112, 238, 1858, 2148
BstX2I RGATCY 3 cut(s) 1273, 2803, 2913
BstXI CCANNNNNNTGG 1 cut(s) 1547
BstYI RGATCY 3 cut(s) 1273, 2803, 2913
BstZ17I GTATAC 2 cut(s) 855, 2385
BstZI CGGCCG 1 cut(s) 240
BtgI CCRYGG 4 cut(s) 1278, 1540, 1620, 2808
BtgZI GCGATG 3 cut(s) 10, 235, 1630
BtrI CACGTC 3 cut(s) 1186, 1483, 2716
BtsCI GGATG 3 cut(s) 180, 477, 2097
BtsI GCAGTG 2 cut(s) 989, 2519
BtsIMutI CAGTG 3 cut(s) 989, 2519, 2990
CaiI CAGNNNCTG 2 cut(s) 933, 2463
Cfr10I RCCGGY 4 cut(s) 533, 1991, 2483, 2677
Cfr9I CCCGGG 3 cut(s) 315, 435, 1935
CsiI ACCWGGT 2 cut(s) 8, 1628
Csp6I GTAC 5 cut(s) 337, 1009, 1426, 2539, 2953
CviQI GTAC 5 cut(s) 337, 1009, 1426, 2539, 2953
DdeI CTNAG 6 cut(s) 937, 1133, 2137, 2467, 2568, 2663
DinI GGCGCC 5 cut(s) 295, 392, 526, 1139, 2669
DpnI GATC 8 cut(s) 20, 989, 1121, 1275, 2519, 2651, 2805, 2915
DpnII GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
DraIII CACNNNGTG 6 cut(s) 1159, 1174, 1207, 2689, 2704, 2737
DrdI GACNNNNNNGTC 2 cut(s) 482, 2102
DseDI GACNNNNNNGTC 2 cut(s) 482, 2102
EaeI YGGCCR 5 cut(s) 240, 324, 993, 1944, 2523
EagI CGGCCG 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 609, 2229
EarI CTCTTC 2 cut(s) 609, 2229
Ecl136II GAGCTC 2 cut(s) 169, 1789
EclXI CGGCCG 1 cut(s) 240
Eco130I CCWWGG 4 cut(s) 1278, 1620, 1941, 2808
Eco147I AGGCCT 2 cut(s) 278, 1898
Eco24I GRGCYC 5 cut(s) 114, 171, 210, 1791, 1830
Eco47I GGWCC 8 cut(s) 871, 1087, 1255, 1486, 2000, 2401, 2617, 2785
Eco52I CGGCCG 1 cut(s) 240
Eco53kI GAGCTC 2 cut(s) 169, 1789
Eco72I CACGTG 2 cut(s) 1204, 2734
EcoICRI GAGCTC 2 cut(s) 169, 1789
EcoO109I RGGNCCY 6 cut(s) 111, 1087, 1255, 1580, 2617, 2785
EcoT14I CCWWGG 4 cut(s) 1278, 1620, 1941, 2808
EcoT38I GRGCYC 5 cut(s) 114, 171, 210, 1791, 1830
EgeI GGCGCC 5 cut(s) 295, 392, 526, 1139, 2669
EheI GGCGCC 5 cut(s) 295, 392, 526, 1139, 2669
ErhI CCWWGG 4 cut(s) 1278, 1620, 1941, 2808
Esp3I CGTCTC 3 cut(s) 43, 1663, 2018
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FaqI GGGAC 4 cut(s) 758, 1268, 1986, 2798
FblI GTMKAC 5 cut(s) 549, 854, 1508, 2169, 2384
FokI GGATG 3 cut(s) 167, 464, 2084
FriOI GRGCYC 5 cut(s) 114, 171, 210, 1791, 1830
FspBI CTAG 1 cut(s) 3078
GsaI CCCAGC 2 cut(s) 1255, 2785
GsuI CTGGAG 5 cut(s) 325, 1442, 1693, 1796, 2969
HaeII RGCGCY 7 cut(s) 297, 394, 528, 534, 649, 1141, 2671
Hin1I GRCGYC 9 cut(s) 294, 391, 525, 537, 1138, 2011, 2145, 2157, 2668
HincII GTYRAC 5 cut(s) 487, 550, 2107, 2170, 3013
HindII GTYRAC 5 cut(s) 487, 550, 2107, 2170, 3013
HindIII AAGCTT 2 cut(s) 905, 2435
HinfI GANTC 8 cut(s) 781, 1386, 1505, 1525, 1614, 2123, 2311, 3052
HpyCH4III ACNGT 4 cut(s) 1513, 1544, 1864, 2000
HpyCH4V TGCA 6 cut(s) 643, 982, 1093, 1534, 2512, 3061
HpyF3I CTNAG 6 cut(s) 937, 1133, 2137, 2467, 2568, 2663
Hsp92I GRCGYC 9 cut(s) 294, 391, 525, 537, 1138, 2011, 2145, 2157, 2668
KasI GGCGCC 5 cut(s) 293, 390, 524, 1137, 2667
KflI GGGWCCC 2 cut(s) 1255, 2785
KroI GCCGGC 4 cut(s) 533, 1991, 2483, 2677
KroNI GCCGGC 4 cut(s) 535, 1993, 2485, 2679
Kzo9I GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
LguI GCTCTTC 2 cut(s) 609, 2229
LweI GCATC 4 cut(s) 234, 1353, 1854, 2880
MabI ACCWGGT 2 cut(s) 8, 1628
MaeI CTAG 1 cut(s) 3078
MaeIII GTNAC 3 cut(s) 599, 1174, 2704
MalI GATC 8 cut(s) 20, 989, 1121, 1275, 2519, 2651, 2805, 2915
MbiI CCGCTC 4 cut(s) 261, 637, 1857, 1881
MboI GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
MflI RGATCY 3 cut(s) 1273, 2803, 2913
MlsI TGGCCA 3 cut(s) 995, 1946, 2525
MluNI TGGCCA 3 cut(s) 995, 1946, 2525
Mly113I GGCGCC 5 cut(s) 294, 391, 525, 1138, 2668
MlyI GAGTC 2 cut(s) 1514, 2132
MmeI TCCRAC 1 cut(s) 1473
Mox20I TGGCCA 3 cut(s) 995, 1946, 2525
MreI CGCCGGCG 2 cut(s) 533, 2677
MroNI GCCGGC 4 cut(s) 533, 1991, 2483, 2677
MroXI GAANNNNTTC 1 cut(s) 423
MscI TGGCCA 3 cut(s) 995, 1946, 2525
MslI CAYNNNNRTG 1 cut(s) 1545
Msp20I TGGCCA 3 cut(s) 995, 1946, 2525
MspA1I CMGCKG 3 cut(s) 1311, 2154, 2841
MvnI CGCG 6 cut(s) 117, 977, 1172, 2058, 2507, 2702
NaeI GCCGGC 4 cut(s) 535, 1993, 2485, 2679
NarI GGCGCC 5 cut(s) 294, 391, 525, 1138, 2668
NciI CCSGG 9 cut(s) 239, 316, 317, 436, 437, 667, 1149, 1936, 1937
NcoI CCATGG 3 cut(s) 1278, 1620, 2808
NdeII GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
NgoMIV GCCGGC 4 cut(s) 533, 1991, 2483, 2677
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 3 cut(s) 599, 1174, 2704
OliI CACNNNNGTG 1 cut(s) 1545
PaeR7I CTCGAG 5 cut(s) 170, 749, 875, 1790, 2279
PceI AGGCCT 2 cut(s) 278, 1898
PciSI GCTCTTC 2 cut(s) 609, 2229
PdiI GCCGGC 4 cut(s) 535, 1993, 2485, 2679
PdmI GAANNNNTTC 1 cut(s) 423
PfeI GAWTC 6 cut(s) 781, 1386, 1525, 1614, 2311, 3052
PleI GAGTC 2 cut(s) 1513, 2131
PluTI GGCGCC 5 cut(s) 297, 394, 528, 1141, 2671
PmaCI CACGTG 2 cut(s) 1204, 2734
PmlI CACGTG 2 cut(s) 1204, 2734
PpsI GAGTC 2 cut(s) 1513, 2131
Ppu21I YACGTR 3 cut(s) 1204, 2734, 3070
PpuMI RGGWCCY 4 cut(s) 1087, 1255, 2617, 2785
Psp124BI GAGCTC 2 cut(s) 171, 1791
Psp5II RGGWCCY 4 cut(s) 1087, 1255, 2617, 2785
PspCI CACGTG 2 cut(s) 1204, 2734
PspFI CCCAGC 2 cut(s) 1251, 2781
PspOMI GGGCCC 3 cut(s) 110, 206, 1826
PspPPI RGGWCCY 4 cut(s) 1087, 1255, 2617, 2785
PspXI VCTCGAGB 5 cut(s) 170, 749, 875, 1790, 2279
PstI CTGCAG 2 cut(s) 984, 2514
PstNI CAGNNNCTG 2 cut(s) 933, 2463
PsuI RGATCY 3 cut(s) 1273, 2803, 2913
RsaI GTAC 5 cut(s) 338, 1010, 1427, 2540, 2954
RsaNI GTAC 5 cut(s) 337, 1009, 1426, 2539, 2953
RseI CAYNNNNRTG 1 cut(s) 1545
SacI GAGCTC 2 cut(s) 171, 1791
SalI GTCGAC 2 cut(s) 548, 2168
SapI GCTCTTC 2 cut(s) 609, 2229
Sau3AI GATC 8 cut(s) 18, 987, 1119, 1273, 2517, 2649, 2803, 2913
ScaI AGTACT 3 cut(s) 338, 1427, 2954
SchI GAGTC 2 cut(s) 1514, 2132
SexAI ACCWGGT 2 cut(s) 8, 1628
SfaNI GCATC 4 cut(s) 234, 1353, 1854, 2880
SfcI CTRYAG 2 cut(s) 980, 2510
SfoI GGCGCC 5 cut(s) 295, 392, 526, 1139, 2669
Sfr274I CTCGAG 5 cut(s) 170, 749, 875, 1790, 2279
SgrAI CRCCGGYG 2 cut(s) 533, 2677
SinI GGWCC 8 cut(s) 871, 1087, 1255, 1486, 2000, 2401, 2617, 2785
SlaI CTCGAG 5 cut(s) 170, 749, 875, 1790, 2279
SmaI CCCGGG 3 cut(s) 317, 437, 1937
SmiMI CAYNNNNRTG 1 cut(s) 1545
SmlI CTYRAG 8 cut(s) 170, 191, 749, 875, 1260, 1303, 1790, 2279
SmoI CTYRAG 8 cut(s) 170, 191, 749, 875, 1260, 1303, 1790, 2279
SseBI AGGCCT 2 cut(s) 278, 1898
SspDI GGCGCC 5 cut(s) 293, 390, 524, 1137, 2667
SspMI CTAG 1 cut(s) 3078
SstI GAGCTC 2 cut(s) 171, 1791
StuI AGGCCT 2 cut(s) 278, 1898
StyI CCWWGG 4 cut(s) 1278, 1620, 1941, 2808
TaaI ACNGT 4 cut(s) 1513, 1544, 1864, 2000
TatI WGTACW 3 cut(s) 336, 1425, 2952
TfiI GAWTC 6 cut(s) 781, 1386, 1525, 1614, 2311, 3052
TscAI CASTG 3 cut(s) 989, 2519, 2990
TseFI GTSAC 3 cut(s) 599, 1174, 2704
Tsp45I GTSAC 3 cut(s) 599, 1174, 2704
TspDTI ATGAA 8 cut(s) 238, 852, 1222, 1535, 2243, 2382, 2752, 3062
TspGWI ACGGA 5 cut(s) 903, 1079, 1926, 2294, 2609
TspMI CCCGGG 3 cut(s) 315, 435, 1935
TspRI CASTG 3 cut(s) 989, 2519, 2990
VpaK11BI GGWCC 8 cut(s) 871, 1087, 1255, 1486, 2000, 2401, 2617, 2785
XapI RAATTY 6 cut(s) 839, 1438, 1451, 2369, 2965, 3081
XhoI CTCGAG 5 cut(s) 170, 749, 875, 1790, 2279
XmaI CCCGGG 3 cut(s) 315, 435, 1935
XmiI GTMKAC 5 cut(s) 549, 854, 1508, 2169, 2384
XmnI GAANNNNTTC 1 cut(s) 423
XspI CTAG 1 cut(s) 3078
ZraI GACGTC 1 cut(s) 2146
ZrmI AGTACT 3 cut(s) 338, 1427, 2954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.