MD01G1204000.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000853
Physical Location & Seq
Forward (+)
29955061 .. 29957446
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1204000.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGAAACCTGGTTCCTTATCCTCATCGCCCTCTGCGTCTCCTTCCTCCTCAGACCCCTTCTCTCTCTATTCCTACCCACTTCCATCTCCAGATCCAATCTCCCTCCCGGGCCTCGCTTCTTCCACATCATCGAAGGCTTCTTATTCCTCCTCAAATCTACCTCGGAGCTCGAGCCTATAATCCGCAAACTCCAGGCCGAATACGGGCCCATCATCTCCCTCCCCATCGGCTCCCGCTCTACCGTCTTCGTCGCCGACCGCTCCCCCGCCCACCAGGCCTTAATCCTGAACGGCGCCGTGTTCGCCGACCGCCCCCAGGCCTTGGCCACCAACAAGTGCGCCAGCAGCAACCAGCACACCATCGCCTCCGCCGTCTACGGCCCCACGTGGCGTCTCCTCCGCCGCAATATCACCTCCGAAATCCTCCACCCTTCCCGCGTTAAATCCTACGGCAGCGCCCGCAAATGGGTTCTCGACATCCTCGTCAACCGCCTCAAAACCGAGTATCAATCCCACTCCGAAGCCGCCGCCGCCGGGGTCAGGGTGGTCGACCACTTCCAATACGCCATGTTCTGCCTCCTGGTTCTGATGTGCTTCGGCGACAAATTGAACGAAGAGCAAATCAAAGAAATCGAGCACGTGCAGCGCCAACTACTTTTGAGTTCCGGGCGGTTCAATATCCTCAATTTCAAGCCGAAATTGACGAAGATTATCTTCAAAAATCGGTGGCGGCAATTCTTCAAAATCCTCGAGGAACGACGAGAAGTGCTCGTCCCTCTGATTCGAGCACGACAAAACAGGGCAAAGCAGGGCAGCAAAACCGACGACAAGGATGATGAATTTGTGTTGTCGTATACAGATACGCTGCTGGACCTCGAGCTTCCCGACGGCAACGAAAAGCGGAAGCTTTCGGAGGGAGAAATGGTTAGCCTCTGCTCAGAGTTTCTGACCGCCGGCACCGACACGACTTCCACCGCGCTGCAGTGGATCATGGCCAACGTAGTGAAGTACCCGCAAGTTCAGGATAAGCTGTTTGCGGAGATTAAAGGGGTGATGGGAGAAACGGAGGACGAGGTGCCGGAGGAGGTCCTGCACAAGCTGCCGTATCTGAAAGCCGTGATCTTGGAGGGGCTGAGGCGCCATCCACCGGCGCACTTTGTGCCGCGCGCGGTGACGCATGACGTGGTTTTGGGCGGATACGTGGTGCCCAAGAACGGGAGTGTTAGTTTCATGGTGGCGGATATAGGGTGGGACCCGCAAGTGTGGGAGGATCCCATGGCTTTCAAGCCGGAGAGGTTCTTGGGCAGCGGCGGAGAAGAAGGGTTCGATTTGAGGGGGAGCAGGGAGATTAAGATGATGCCGTTTGGGGCAGGGAGGAGGATCTGTCCAGGGTCGGGATTGGCGGTGCTTCACCTGGAGTACTTTGTGGCGAATTTGGTGTGGAAATTTGAGTGGAGAACTGTGGAGGGAGATGACGTGGACCTGTCAGAGAAGCAGGAGTTTACTATAGTGATGAAGAATCCATTGCATGCCCACGGTGGTGGTTCCGGCACCCAACAAATTGTGATCGAAGGCCCCTTATGCGAGGATTATTACAAGATCTGGGAGTATCTCTACTCACAATTTTATTTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

546

Amino Acids

61.89

Weight (kDa)

6.72

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 508 2.1e-90 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 482
AbsI CCTCGAGG 1 cut(s) 749
AccB1I GGYRCC 6 cut(s) 293, 956, 1075, 1137, 1204, 1550
AccB7I CCANNNNNTGG 1 cut(s) 322
AccBSI CCGCTC 2 cut(s) 237, 261
AccI GTMKAC 3 cut(s) 375, 549, 854
AccII CGCG 5 cut(s) 438, 977, 1165, 1167, 1169
AclWI GGATC 5 cut(s) 87, 995, 1265, 1278, 1388
AcoI YGGCCR 2 cut(s) 324, 993
AcsI RAATTY 3 cut(s) 839, 1432, 1445
AcvI CACGTG 2 cut(s) 387, 640
AcyI GRCGYC 3 cut(s) 294, 391, 1138
AdeI CACNNNGTG 1 cut(s) 1159
AfaI GTAC 2 cut(s) 1010, 1421
AgsI TTSAA 6 cut(s) 610, 676, 691, 718, 742, 1285
AjiI CACGTC 2 cut(s) 1183, 1477
AjnI CCWGG 7 cut(s) 8, 192, 273, 315, 579, 1387, 1413
AleI CACNNNNGTG 1 cut(s) 1539
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 5 cut(s) 169, 880, 907, 1030, 1099
AluI AGCT 5 cut(s) 169, 880, 907, 1030, 1099
Alw21I GWGCWC 4 cut(s) 171, 639, 771, 790
Alw26I GTCTC 2 cut(s) 43, 398
AlwI GGATC 5 cut(s) 87, 995, 1265, 1278, 1388
Ama87I CYCGRG 4 cut(s) 107, 170, 749, 875
AoxI GGCC 9 cut(s) 110, 195, 206, 276, 318, 324, 379, 993, 1573
ApaI GGGCCC 1 cut(s) 210
ApeKI GCWGC 8 cut(s) 345, 453, 643, 813, 865, 979, 1099, 1305
ApoI RAATTY 3 cut(s) 839, 1432, 1445
AspLEI GCGC 9 cut(s) 296, 341, 458, 648, 979, 1140, 1153, 1167, 1169
AspS9I GGNCC 9 cut(s) 110, 206, 207, 380, 871, 1087, 1252, 1480, 1574
AsuC2I CCSGG 4 cut(s) 108, 109, 535, 667
AsuHPI GGTGA 4 cut(s) 403, 1063, 1183, 1403
AvaI CYCGRG 4 cut(s) 107, 170, 749, 875
AvaII GGWCC 4 cut(s) 871, 1087, 1252, 1480
BaeGI GKGCMC 2 cut(s) 210, 1209
BalI TGGCCA 2 cut(s) 326, 995
BamHI GGATCC 1 cut(s) 1270
BanI GGYRCC 6 cut(s) 293, 956, 1075, 1137, 1204, 1550
BanII GRGCYC 2 cut(s) 171, 210
BbrPI CACGTG 2 cut(s) 387, 640
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 4 cut(s) 171, 639, 771, 790
BbvCI CCTCAGC 1 cut(s) 1133
BbvI GCAGC 8 cut(s) 357, 465, 655, 825, 852, 966, 1086, 1317
BccI CCATC 6 cut(s) 92, 218, 233, 368, 1048, 1149
BceAI ACGGC 9 cut(s) 281, 307, 356, 394, 466, 904, 1087, 1100, 1345
BciT130I CCWGG 7 cut(s) 10, 194, 275, 317, 581, 1389, 1415
BciVI GTATCC 1 cut(s) 1190
BcnI CCSGG 4 cut(s) 108, 109, 535, 667
BcoDI GTCTC 2 cut(s) 43, 398
BfmI CTRYAG 2 cut(s) 980, 1506
BfoI RGCGCY 4 cut(s) 297, 459, 649, 1141
BfuI GTATCC 1 cut(s) 1190
BglI GCCNNNNNGGC 1 cut(s) 275
BglII AGATCT 1 cut(s) 1599
BmcAI AGTACT 1 cut(s) 1421
Bme18I GGWCC 4 cut(s) 871, 1087, 1252, 1480
BmeT110I CYCGRG 4 cut(s) 107, 170, 749, 875
BmgBI CACGTC 2 cut(s) 1183, 1477
BmgT120I GGNCC 9 cut(s) 110, 206, 207, 380, 871, 1087, 1252, 1480, 1574
BmsI GCATC 1 cut(s) 1347
BpiI GAAGAC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 753, 785
BpmI CTGGAG 3 cut(s) 73, 176, 1436
Bpu10I CCTNAGC 1 cut(s) 1133
BpuMI CCSGG 4 cut(s) 108, 109, 535, 667
BsaAI YACGTR 3 cut(s) 387, 640, 1201
BsaHI GRCGYC 3 cut(s) 294, 391, 1138
BsaJI CCNNGG 8 cut(s) 107, 162, 315, 321, 534, 1275, 1388, 1534
BsaXI ACNNNNNCTCC 8 cut(s) 909, 939, 1210, 1240, 1369, 1399, 1461, 1491
Bse118I RCCGGY 2 cut(s) 953, 1147
Bse3DI GCAATG 1 cut(s) 1523
BseBI CCWGG 7 cut(s) 10, 194, 275, 317, 581, 1389, 1415
BseDI CCNNGG 8 cut(s) 107, 162, 315, 321, 534, 1275, 1388, 1534
BseGI GGATG 3 cut(s) 477, 838, 1141
BseMI GCAATG 1 cut(s) 1523
BseMII CTCAG 3 cut(s) 64, 951, 1124
BsePI GCGCGC 1 cut(s) 1165
BseRI GAGGAG 5 cut(s) 38, 140, 386, 1097, 1390
BseSI GKGCMC 2 cut(s) 210, 1209
BseXI GCAGC 8 cut(s) 357, 465, 655, 825, 852, 966, 1086, 1317
BsgI GTGCAG 2 cut(s) 662, 1076
Bsh1236I CGCG 5 cut(s) 438, 977, 1165, 1167, 1169
Bsh1285I CGRYCG 2 cut(s) 259, 310
BshFI GGCC 9 cut(s) 112, 197, 208, 278, 320, 326, 381, 995, 1575
BshNI GGYRCC 6 cut(s) 293, 956, 1075, 1137, 1204, 1550
BsiEI CGRYCG 2 cut(s) 259, 310
BsiHKAI GWGCWC 4 cut(s) 171, 639, 771, 790
BsiHKCI CYCGRG 4 cut(s) 107, 170, 749, 875
BsiSI CCGG 8 cut(s) 108, 534, 666, 954, 1079, 1148, 1289, 1548
BslFI GGGAC 2 cut(s) 758, 1265
BsmAI GTCTC 2 cut(s) 43, 398
BsmBI CGTCTC 2 cut(s) 43, 398
BsmFI GGGAC 2 cut(s) 758, 1265
BsnI GGCC 9 cut(s) 112, 197, 208, 278, 320, 326, 381, 995, 1575
BsoBI CYCGRG 4 cut(s) 107, 170, 749, 875
Bsp120I GGGCCC 1 cut(s) 206
Bsp1286I GDGCHC 6 cut(s) 171, 210, 639, 771, 790, 1209
Bsp143I GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
Bsp19I CCATGG 1 cut(s) 1275
BspANI GGCC 9 cut(s) 112, 197, 208, 278, 320, 326, 381, 995, 1575
BspCNI CTCAG 3 cut(s) 63, 950, 1125
BspFNI CGCG 5 cut(s) 438, 977, 1165, 1167, 1169
BspMAI CTGCAG 1 cut(s) 984
BspPI GGATC 5 cut(s) 87, 995, 1265, 1278, 1388
BspQI GCTCTTC 1 cut(s) 609
BspT107I GGYRCC 6 cut(s) 293, 956, 1075, 1137, 1204, 1550
BsrBI CCGCTC 2 cut(s) 237, 261
BsrDI GCAATG 1 cut(s) 1523
BsrFI RCCGGY 2 cut(s) 953, 1147
BssAI RCCGGY 2 cut(s) 953, 1147
BssECI CCNNGG 8 cut(s) 107, 162, 315, 321, 534, 1275, 1388, 1534
BssHII GCGCGC 1 cut(s) 1165
BssMI GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
BssNAI GTATAC 1 cut(s) 855
BssNI GRCGYC 3 cut(s) 294, 391, 1138
BssT1I CCWWGG 2 cut(s) 321, 1275
Bst1107I GTATAC 1 cut(s) 855
Bst2UI CCWGG 7 cut(s) 10, 194, 275, 317, 581, 1389, 1415
Bst4CI ACNGT 3 cut(s) 244, 1462, 1538
Bst6I CTCTTC 1 cut(s) 609
BstACI GRCGYC 3 cut(s) 294, 391, 1138
BstAPI GCANNNNNTGC 2 cut(s) 1099, 1159
BstBAI YACGTR 3 cut(s) 387, 640, 1201
BstC8I GCNNGC 5 cut(s) 343, 460, 955, 1167, 1530
BstDEI CTNAG 3 cut(s) 50, 937, 1133
BstDSI CCRYGG 2 cut(s) 1275, 1534
BstF5I GGATG 3 cut(s) 477, 838, 1141
BstFNI CGCG 5 cut(s) 438, 977, 1165, 1167, 1169
BstH2I RGCGCY 4 cut(s) 297, 459, 649, 1141
BstHHI GCGC 9 cut(s) 296, 341, 458, 648, 979, 1140, 1153, 1167, 1169
BstKTI GATC 7 cut(s) 95, 990, 1122, 1273, 1383, 1569, 1602
BstMAI GTCTC 2 cut(s) 43, 398
BstMBI GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
BstMCI CGRYCG 2 cut(s) 259, 310
BstMWI GCNNNNNNNGC 9 cut(s) 275, 302, 345, 459, 530, 643, 1099, 1159, 1581
BstNI CCWGG 7 cut(s) 10, 194, 275, 317, 581, 1389, 1415
BstNSI RCATGY 1 cut(s) 1532
BstSFI CTRYAG 2 cut(s) 980, 1506
BstSLI GKGCMC 2 cut(s) 210, 1209
BstUI CGCG 5 cut(s) 438, 977, 1165, 1167, 1169
BstV1I GCAGC 8 cut(s) 357, 465, 655, 825, 852, 966, 1086, 1317
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 4 cut(s) 92, 1270, 1380, 1599
BstXI CCANNNNNNTGG 1 cut(s) 1541
BstYI RGATCY 4 cut(s) 92, 1270, 1380, 1599
BstZ17I GTATAC 1 cut(s) 855
BsuI GTATCC 1 cut(s) 1190
BsuRI GGCC 9 cut(s) 112, 197, 208, 278, 320, 326, 381, 995, 1575
BtgI CCRYGG 2 cut(s) 1275, 1534
BtgZI GCGATG 2 cut(s) 10, 346
BtrI CACGTC 2 cut(s) 1183, 1477
BtsCI GGATG 3 cut(s) 477, 838, 1141
BtsI GCAGTG 1 cut(s) 989
BtsIMutI CAGTG 1 cut(s) 989
Cac8I GCNNGC 5 cut(s) 343, 460, 955, 1167, 1530
CfoI GCGC 9 cut(s) 296, 341, 458, 648, 979, 1140, 1153, 1167, 1169
Cfr10I RCCGGY 2 cut(s) 953, 1147
Cfr13I GGNCC 9 cut(s) 110, 206, 207, 380, 871, 1087, 1252, 1480, 1574
Cfr9I CCCGGG 1 cut(s) 107
CseI GACGC 3 cut(s) 25, 380, 1183
CsiI ACCWGGT 1 cut(s) 8
Csp6I GTAC 2 cut(s) 1009, 1420
CviAII CATG 6 cut(s) 568, 991, 1178, 1231, 1276, 1529
CviQI GTAC 2 cut(s) 1009, 1420
DdeI CTNAG 3 cut(s) 50, 937, 1133
DinI GGCGCC 2 cut(s) 295, 1139
DpnI GATC 7 cut(s) 94, 989, 1121, 1272, 1382, 1568, 1601
DpnII GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
DraIII CACNNNGTG 1 cut(s) 1159
DrdI GACNNNNNNGTC 1 cut(s) 482
DseDI GACNNNNNNGTC 1 cut(s) 482
EaeI YGGCCR 2 cut(s) 324, 993
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
EciI GGCGGA 5 cut(s) 358, 389, 1209, 1253, 1326
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 321, 1275
Eco147I AGGCCT 2 cut(s) 278, 320
Eco24I GRGCYC 2 cut(s) 171, 210
Eco47I GGWCC 4 cut(s) 871, 1087, 1252, 1480
Eco53kI GAGCTC 1 cut(s) 169
Eco72I CACGTG 2 cut(s) 387, 640
Eco88I CYCGRG 4 cut(s) 107, 170, 749, 875
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 3 cut(s) 1087, 1252, 1574
EcoRII CCWGG 7 cut(s) 8, 192, 273, 315, 579, 1387, 1413
EcoT14I CCWWGG 2 cut(s) 321, 1275
EcoT38I GRGCYC 2 cut(s) 171, 210
EgeI GGCGCC 2 cut(s) 295, 1139
EheI GGCGCC 2 cut(s) 295, 1139
ErhI CCWWGG 2 cut(s) 321, 1275
Esp3I CGTCTC 2 cut(s) 43, 398
FaeI CATG 6 cut(s) 571, 994, 1181, 1234, 1279, 1532
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FaqI GGGAC 2 cut(s) 758, 1265
FatI CATG 6 cut(s) 567, 990, 1177, 1230, 1275, 1528
FauI CCCGC 6 cut(s) 242, 274, 443, 467, 1020, 1263
FblI GTMKAC 3 cut(s) 375, 549, 854
FokI GGATG 3 cut(s) 464, 845, 1128
FriOI GRGCYC 2 cut(s) 171, 210
GlaI GCGC 9 cut(s) 295, 340, 457, 647, 978, 1139, 1152, 1166, 1168
GsuI CTGGAG 3 cut(s) 73, 176, 1436
HaeII RGCGCY 4 cut(s) 297, 459, 649, 1141
HaeIII GGCC 9 cut(s) 112, 197, 208, 278, 320, 326, 381, 995, 1575
HapII CCGG 8 cut(s) 108, 534, 666, 954, 1079, 1148, 1289, 1548
HgaI GACGC 3 cut(s) 25, 380, 1183
HhaI GCGC 9 cut(s) 296, 341, 458, 648, 979, 1140, 1153, 1167, 1169
Hin1I GRCGYC 3 cut(s) 294, 391, 1138
Hin1II CATG 6 cut(s) 571, 994, 1181, 1234, 1279, 1532
Hin6I GCGC 9 cut(s) 294, 339, 456, 646, 977, 1138, 1151, 1165, 1167
HinP1I GCGC 9 cut(s) 294, 339, 456, 646, 977, 1138, 1151, 1165, 1167
HincII GTYRAC 2 cut(s) 487, 550
HindII GTYRAC 2 cut(s) 487, 550
HindIII AAGCTT 1 cut(s) 905
HinfI GANTC 2 cut(s) 781, 1519
HpaII CCGG 8 cut(s) 108, 534, 666, 954, 1079, 1148, 1289, 1548
HphI GGTGA 4 cut(s) 403, 1063, 1183, 1403
Hpy166II GTNNAC 6 cut(s) 376, 487, 550, 855, 1480, 1503
Hpy188III TCNNGA 6 cut(s) 90, 286, 473, 884, 1022, 1395
Hpy8I GTNNAC 6 cut(s) 376, 487, 550, 855, 1480, 1503
Hpy99I CGWCG 4 cut(s) 254, 762, 827, 890
HpyAV CCTTC 6 cut(s) 52, 68, 128, 441, 1313, 1565
HpyCH4III ACNGT 3 cut(s) 244, 1462, 1538
HpyCH4IV ACGT 6 cut(s) 386, 639, 999, 1182, 1200, 1476
HpyCH4V TGCA 4 cut(s) 643, 982, 1093, 1528
HpyF10VI GCNNNNNNNGC 9 cut(s) 275, 302, 345, 459, 530, 643, 1099, 1159, 1581
HpyF3I CTNAG 3 cut(s) 50, 937, 1133
HpySE526I ACGT 6 cut(s) 386, 639, 999, 1182, 1200, 1476
Hsp92I GRCGYC 3 cut(s) 294, 391, 1138
Hsp92II CATG 6 cut(s) 571, 994, 1181, 1234, 1279, 1532
HspAI GCGC 9 cut(s) 294, 339, 456, 646, 977, 1138, 1151, 1165, 1167
KasI GGCGCC 2 cut(s) 293, 1137
KflI GGGWCCC 1 cut(s) 1252
KroI GCCGGC 1 cut(s) 953
KroNI GCCGGC 1 cut(s) 955
Kzo9I GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 4 cut(s) 166, 236, 266, 1338
Lsp1109I GCAGC 8 cut(s) 357, 465, 655, 825, 852, 966, 1086, 1317
LweI GCATC 1 cut(s) 1347
MabI ACCWGGT 1 cut(s) 8
MaeII ACGT 6 cut(s) 386, 639, 999, 1182, 1200, 1476
MaeIII GTNAC 1 cut(s) 1171
MalI GATC 7 cut(s) 94, 989, 1121, 1272, 1382, 1568, 1601
MauBI CGCGCGCG 1 cut(s) 1165
MbiI CCGCTC 2 cut(s) 237, 261
MboI GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
MboII GAAGA 8 cut(s) 112, 238, 626, 706, 718, 730, 1328, 1528
MflI RGATCY 4 cut(s) 92, 1270, 1380, 1599
MhlI GDGCHC 6 cut(s) 171, 210, 639, 771, 790, 1209
MlsI TGGCCA 2 cut(s) 326, 995
MluCI AATT 9 cut(s) 605, 685, 698, 734, 839, 1432, 1445, 1560, 1622
MluNI TGGCCA 2 cut(s) 326, 995
Mly113I GGCGCC 2 cut(s) 294, 1138
Mox20I TGGCCA 2 cut(s) 326, 995
MroNI GCCGGC 1 cut(s) 953
MscI TGGCCA 2 cut(s) 326, 995
MseI TTAA 5 cut(s) 281, 441, 1044, 1350, 1636
MslI CAYNNNNRTG 1 cut(s) 1539
Msp20I TGGCCA 2 cut(s) 326, 995
MspA1I CMGCKG 1 cut(s) 1308
MspI CCGG 8 cut(s) 108, 534, 666, 954, 1079, 1148, 1289, 1548
MvaI CCWGG 7 cut(s) 10, 194, 275, 317, 581, 1389, 1415
MvnI CGCG 5 cut(s) 438, 977, 1165, 1167, 1169
MwoI GCNNNNNNNGC 9 cut(s) 275, 302, 345, 459, 530, 643, 1099, 1159, 1581
NaeI GCCGGC 1 cut(s) 955
NarI GGCGCC 2 cut(s) 294, 1138
NciI CCSGG 4 cut(s) 108, 109, 535, 667
NcoI CCATGG 1 cut(s) 1275
NdeII GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
NgoMIV GCCGGC 1 cut(s) 953
NlaIII CATG 6 cut(s) 571, 994, 1181, 1234, 1279, 1532
NmuCI GTSAC 1 cut(s) 1171
NspI RCATGY 1 cut(s) 1532
OliI CACNNNNGTG 1 cut(s) 1539
PaeI GCATGC 1 cut(s) 1532
PaeR7I CTCGAG 3 cut(s) 170, 749, 875
PauI GCGCGC 1 cut(s) 1165
PceI AGGCCT 2 cut(s) 278, 320
PciSI GCTCTTC 1 cut(s) 609
PcsI WCGNNNNNNNCGW 4 cut(s) 33, 369, 480, 882
PdiI GCCGGC 1 cut(s) 955
PfeI GAWTC 2 cut(s) 781, 1519
PflMI CCANNNNNTGG 1 cut(s) 322
PluTI GGCGCC 2 cut(s) 297, 1141
PmaCI CACGTG 2 cut(s) 387, 640
PmlI CACGTG 2 cut(s) 387, 640
Ppu21I YACGTR 3 cut(s) 387, 640, 1201
PpuMI RGGWCCY 2 cut(s) 1087, 1252
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 2 cut(s) 1087, 1252
Psp6I CCWGG 7 cut(s) 8, 192, 273, 315, 579, 1387, 1413
PspCI CACGTG 2 cut(s) 387, 640
PspGI CCWGG 7 cut(s) 8, 192, 273, 315, 579, 1387, 1413
PspOMI GGGCCC 1 cut(s) 206
PspPI GGNCC 9 cut(s) 110, 206, 207, 380, 871, 1087, 1252, 1480, 1574
PspPPI RGGWCCY 2 cut(s) 1087, 1252
PspXI VCTCGAGB 3 cut(s) 170, 749, 875
PstI CTGCAG 1 cut(s) 984
PsuI RGATCY 4 cut(s) 92, 1270, 1380, 1599
PteI GCGCGC 1 cut(s) 1165
RsaI GTAC 2 cut(s) 1010, 1421
RsaNI GTAC 2 cut(s) 1009, 1420
RseI CAYNNNNRTG 1 cut(s) 1539
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 548
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 5 cut(s) 281, 441, 1044, 1350, 1636
Sau3AI GATC 7 cut(s) 92, 987, 1119, 1270, 1380, 1566, 1599
Sau96I GGNCC 9 cut(s) 110, 206, 207, 380, 871, 1087, 1252, 1480, 1574
ScaI AGTACT 1 cut(s) 1421
SduI GDGCHC 6 cut(s) 171, 210, 639, 771, 790, 1209
SexAI ACCWGGT 1 cut(s) 8
SfaNI GCATC 1 cut(s) 1347
SfcI CTRYAG 2 cut(s) 980, 1506
SfoI GGCGCC 2 cut(s) 295, 1139
Sfr274I CTCGAG 3 cut(s) 170, 749, 875
SgrAI CRCCGGYG 1 cut(s) 1147
SinI GGWCC 4 cut(s) 871, 1087, 1252, 1480
SlaI CTCGAG 3 cut(s) 170, 749, 875
SmaI CCCGGG 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 1539
SmlI CTYRAG 3 cut(s) 170, 749, 875
SmoI CTYRAG 3 cut(s) 170, 749, 875
SphI GCATGC 1 cut(s) 1532
Sse9I AATT 9 cut(s) 605, 685, 698, 734, 839, 1432, 1445, 1560, 1622
SseBI AGGCCT 2 cut(s) 278, 320
SspDI GGCGCC 2 cut(s) 293, 1137
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 2 cut(s) 278, 320
StyI CCWWGG 2 cut(s) 321, 1275
TaaI ACNGT 3 cut(s) 244, 1462, 1538
TaiI ACGT 6 cut(s) 389, 642, 1002, 1185, 1203, 1479
TasI AATT 9 cut(s) 605, 685, 698, 734, 839, 1432, 1445, 1560, 1622
TatI WGTACW 1 cut(s) 1419
TauI GCSGC 7 cut(s) 405, 527, 530, 533, 733, 1165, 1311
TfiI GAWTC 2 cut(s) 781, 1519
Tru1I TTAA 5 cut(s) 281, 441, 1044, 1350, 1636
Tru9I TTAA 5 cut(s) 281, 441, 1044, 1350, 1636
TscAI CASTG 1 cut(s) 989
TseFI GTSAC 1 cut(s) 1171
TseI GCWGC 8 cut(s) 345, 453, 643, 813, 865, 979, 1099, 1305
Tsp45I GTSAC 1 cut(s) 1171
TspDTI ATGAA 3 cut(s) 852, 1219, 1529
TspGWI ACGGA 1 cut(s) 1079
TspMI CCCGGG 1 cut(s) 107
TspRI CASTG 1 cut(s) 989
Van91I CCANNNNNTGG 1 cut(s) 322
VpaK11BI GGWCC 4 cut(s) 871, 1087, 1252, 1480
XapI RAATTY 3 cut(s) 839, 1432, 1445
XceI RCATGY 1 cut(s) 1532
XhoI CTCGAG 3 cut(s) 170, 749, 875
XmaI CCCGGG 1 cut(s) 107
XmiI GTMKAC 3 cut(s) 375, 549, 854
ZrmI AGTACT 1 cut(s) 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.