pycom01g21300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000853
Physical Location & Seq
Forward (+)
19413715 .. 19414506
792 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21300.1

Sequence Viewer

Length: 792 bp
ATGTTCTGCCTCCTGGTTCTGATGTGCTTCGGTGACAAATTGAACGAAGAGCAAATCAAAGAAATCGAGCGGGTGCAGCGCCAACTACTTTTGAGTTCCGGGCGGTTCAATATCCTCAATTTCATGCCTAAATTGACCAAGATTATCTTCAAAAATCGGTGGCGGCAATTCTTCAAAATCCTCGAGGAACGACGAGAAGTGCTCGTCCCTCTGATTCGAGCACGACAAAACAAGGCAAAGCAGGGCAGCAAAAACGACGACAAAGACGATGAATTTGTGTTGTCGTATACTGATACGCTGCTGGACCTCGAGCTTCCCGACGGCAACGAAAAGCGGAAGCTTTCGGAGGGAGAAATGGTCAGCCTCTGCTCAGAGTTTCTGAACGCCGGCACCGAGACGACTTCCACCGCGCTGCAGTGGATCATGGCCAATGTAGTGAAGTACCCGCAAGTTCAGGAGAAGCTGTTTATGCAGATTAAAGGGGTGATGGGAGAAACGGAGGATATGGTGCGGGAGGAGGTCCTGCACAAGCTGCCGTATCTGAAAGCCGTGATCTTGGAGGGGCTGAGGCGCCACCCGCCGGGGCACTTTCTGCTGCCGCACGCGGTGACGCATGACGTGGTTTTGGGCGGACACGTGGTGCCCAAGAACGGGAGTGTTAATTTCATGGTTGCGGATATAGGCTGGGACCCGCAAGTGTGGGAACGATCCCATGGCGTTCAAGCCGGAGAGGTTCTTGAGCAGGGGCGGAGAAGAAGAAGGGTTCGATTTGACGGGGAGCAGGGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

264

Amino Acids

30.41

Weight (kDa)

7.07

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 8 - 236 2.9e-42 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 182
AccB1I GGYRCC 3 cut(s) 389, 570, 640
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 287
AccII CGCG 2 cut(s) 410, 605
AclWI GGATC 2 cut(s) 428, 702
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 272
AcvI CACGTG 1 cut(s) 637
AcyI GRCGYC 1 cut(s) 571
AdeI CACNNNGTG 2 cut(s) 607, 640
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 3 cut(s) 580, 650, 651
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 5 cut(s) 43, 109, 151, 175, 722
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 1 cut(s) 12
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 4 cut(s) 313, 340, 463, 532
AluI AGCT 4 cut(s) 313, 340, 463, 532
Alw21I GWGCWC 2 cut(s) 204, 223
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 2 cut(s) 428, 702
AlwNI CAGNNNCTG 1 cut(s) 366
Ama87I CYCGRG 2 cut(s) 182, 308
AoxI GGCC 1 cut(s) 426
ApeKI GCWGC 6 cut(s) 76, 246, 298, 412, 532, 595
ApoI RAATTY 1 cut(s) 272
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspLEI GCGC 3 cut(s) 81, 412, 573
AspS9I GGNCC 3 cut(s) 304, 520, 688
AsuC2I CCSGG 2 cut(s) 100, 582
AsuHPI GGTGA 3 cut(s) 44, 496, 619
AvaI CYCGRG 2 cut(s) 182, 308
AvaII GGWCC 3 cut(s) 304, 520, 688
BaeGI GKGCMC 2 cut(s) 588, 645
BalI TGGCCA 1 cut(s) 428
BanI GGYRCC 3 cut(s) 389, 570, 640
BbrPI CACGTG 1 cut(s) 637
Bbv12I GWGCWC 2 cut(s) 204, 223
BbvCI CCTCAGC 1 cut(s) 566
BbvI GCAGC 6 cut(s) 88, 258, 285, 399, 519, 582
BccI CCATC 1 cut(s) 481
BceAI ACGGC 3 cut(s) 337, 520, 533
BciT130I CCWGG 1 cut(s) 14
BcnI CCSGG 2 cut(s) 100, 582
BcoDI GTCTC 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 413
BfoI RGCGCY 2 cut(s) 82, 574
BisI GCNGC 8 cut(s) 77, 164, 247, 299, 413, 533, 596, 599
BlsI GCNGC 8 cut(s) 78, 165, 248, 300, 414, 534, 597, 600
Bme1390I CCNGG 3 cut(s) 14, 100, 582
Bme18I GGWCC 3 cut(s) 304, 520, 688
BmeT110I CYCGRG 2 cut(s) 182, 308
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 3 cut(s) 304, 520, 688
BmiI GGNNCC 5 cut(s) 391, 572, 642, 689, 690
BmrFI CCNGG 3 cut(s) 14, 100, 582
BplI GAGNNNNNCTC 2 cut(s) 186, 218
Bpu10I CCTNAGC 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 758
BpuMI CCSGG 2 cut(s) 100, 582
BsaAI YACGTR 1 cut(s) 637
BsaHI GRCGYC 1 cut(s) 571
BsaJI CCNNGG 2 cut(s) 581, 712
BsaXI ACNNNNNCTCC 4 cut(s) 342, 372, 449, 479
Bsc4I CCNNNNNNNGG 3 cut(s) 580, 650, 651
Bse118I RCCGGY 1 cut(s) 386
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 581, 712
BseLI CCNNNNNNNGG 3 cut(s) 580, 650, 651
BseMII CTCAG 2 cut(s) 384, 557
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 2 cut(s) 588, 645
BseXI GCAGC 6 cut(s) 88, 258, 285, 399, 519, 582
BseYI CCCAGC 1 cut(s) 684
BsgI GTGCAG 2 cut(s) 95, 509
Bsh1236I CGCG 2 cut(s) 410, 605
BshFI GGCC 1 cut(s) 428
BshNI GGYRCC 3 cut(s) 389, 570, 640
BsiHKAI GWGCWC 2 cut(s) 204, 223
BsiHKCI CYCGRG 2 cut(s) 182, 308
BsiSI CCGG 4 cut(s) 99, 387, 581, 726
BslFI GGGAC 2 cut(s) 191, 701
BslI CCNNNNNNNGG 3 cut(s) 580, 650, 651
BsmAI GTCTC 1 cut(s) 389
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 2 cut(s) 191, 701
BsnI GGCC 1 cut(s) 428
BsoBI CYCGRG 2 cut(s) 182, 308
Bsp1286I GDGCHC 4 cut(s) 204, 223, 588, 645
Bsp143I GATC 3 cut(s) 420, 552, 707
Bsp19I CCATGG 1 cut(s) 712
BspANI GGCC 1 cut(s) 428
BspCNI CTCAG 2 cut(s) 383, 558
BspFNI CGCG 2 cut(s) 410, 605
BspLI GGNNCC 5 cut(s) 391, 572, 642, 689, 690
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 2 cut(s) 428, 702
BspQI GCTCTTC 1 cut(s) 42
BspT107I GGYRCC 3 cut(s) 389, 570, 640
BsrBI CCGCTC 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 2 cut(s) 581, 712
BssMI GATC 3 cut(s) 420, 552, 707
BssNAI GTATAC 1 cut(s) 288
BssNI GRCGYC 1 cut(s) 571
BssT1I CCWWGG 1 cut(s) 712
Bst1107I GTATAC 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 14
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 571
BstAPI GCANNNNNTGC 2 cut(s) 532, 592
BstBAI YACGTR 1 cut(s) 637
BstC8I GCNNGC 2 cut(s) 388, 603
BstDEI CTNAG 2 cut(s) 370, 566
BstDSI CCRYGG 1 cut(s) 712
BstFNI CGCG 2 cut(s) 410, 605
BstH2I RGCGCY 2 cut(s) 82, 574
BstHHI GCGC 3 cut(s) 81, 412, 573
BstKTI GATC 3 cut(s) 423, 555, 710
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 3 cut(s) 420, 552, 707
BstMWI GCNNNNNNNGC 5 cut(s) 76, 469, 532, 577, 592
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 3 cut(s) 12, 98, 580
BstSFI CTRYAG 1 cut(s) 413
BstSLI GKGCMC 2 cut(s) 588, 645
BstUI CGCG 2 cut(s) 410, 605
BstV1I GCAGC 6 cut(s) 88, 258, 285, 399, 519, 582
BstZ17I GTATAC 1 cut(s) 288
BsuRI GGCC 1 cut(s) 428
BtgI CCRYGG 1 cut(s) 712
BtrI CACGTC 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 388, 603
CaiI CAGNNNCTG 1 cut(s) 366
CfoI GCGC 3 cut(s) 81, 412, 573
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 3 cut(s) 304, 520, 688
CseI GACGC 1 cut(s) 619
Csp6I GTAC 1 cut(s) 442
CviAII CATG 5 cut(s) 124, 424, 614, 667, 713
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 2 cut(s) 370, 566
DinI GGCGCC 1 cut(s) 572
DpnI GATC 3 cut(s) 422, 554, 709
DpnII GATC 3 cut(s) 420, 552, 707
DraIII CACNNNGTG 2 cut(s) 607, 640
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
EciI GGCGGA 2 cut(s) 645, 763
Eco130I CCWWGG 1 cut(s) 712
Eco47I GGWCC 3 cut(s) 304, 520, 688
Eco72I CACGTG 1 cut(s) 637
Eco88I CYCGRG 2 cut(s) 182, 308
EcoO109I RGGNCCY 2 cut(s) 520, 688
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 712
EgeI GGCGCC 1 cut(s) 572
EheI GGCGCC 1 cut(s) 572
ErhI CCWWGG 1 cut(s) 712
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 5 cut(s) 127, 427, 617, 670, 716
FaiI YATR 9 cut(s) 125, 288, 425, 470, 506, 615, 668, 680, 714
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 2 cut(s) 191, 701
FatI CATG 5 cut(s) 123, 423, 613, 666, 712
FauI CCCGC 5 cut(s) 63, 453, 504, 585, 699
FblI GTMKAC 1 cut(s) 287
Fnu4HI GCNGC 8 cut(s) 77, 164, 247, 299, 413, 533, 596, 599
Fsp4HI GCNGC 8 cut(s) 77, 164, 247, 299, 413, 533, 596, 599
GlaI GCGC 3 cut(s) 80, 411, 572
GluI GCNGC 8 cut(s) 77, 164, 247, 299, 413, 533, 596, 599
GsaI CCCAGC 1 cut(s) 688
HaeII RGCGCY 2 cut(s) 82, 574
HaeIII GGCC 1 cut(s) 428
HapII CCGG 4 cut(s) 99, 387, 581, 726
HgaI GACGC 1 cut(s) 619
HhaI GCGC 3 cut(s) 81, 412, 573
Hin1I GRCGYC 1 cut(s) 571
Hin1II CATG 5 cut(s) 127, 427, 617, 670, 716
Hin6I GCGC 3 cut(s) 79, 410, 571
HinP1I GCGC 3 cut(s) 79, 410, 571
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 1 cut(s) 214
HpaII CCGG 4 cut(s) 99, 387, 581, 726
HphI GGTGA 3 cut(s) 44, 496, 619
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 6 cut(s) 21, 213, 346, 373, 381, 543
Hpy188III TCNNGA 3 cut(s) 317, 455, 737
Hpy8I GTNNAC 1 cut(s) 288
Hpy99I CGWCG 3 cut(s) 195, 260, 323
HpyAV CCTTC 1 cut(s) 753
HpyCH4IV ACGT 2 cut(s) 618, 636
HpyCH4V TGCA 4 cut(s) 76, 415, 472, 526
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 469, 532, 577, 592
HpyF3I CTNAG 2 cut(s) 370, 566
HpySE526I ACGT 2 cut(s) 618, 636
Hsp92I GRCGYC 1 cut(s) 571
Hsp92II CATG 5 cut(s) 127, 427, 617, 670, 716
HspAI GCGC 3 cut(s) 79, 410, 571
KasI GGCGCC 1 cut(s) 570
KflI GGGWCCC 1 cut(s) 688
KroI GCCGGC 1 cut(s) 386
KroNI GCCGGC 1 cut(s) 388
Kzo9I GATC 3 cut(s) 420, 552, 707
LguI GCTCTTC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 778
Lsp1109I GCAGC 6 cut(s) 88, 258, 285, 399, 519, 582
MaeII ACGT 2 cut(s) 618, 636
MaeIII GTNAC 2 cut(s) 32, 607
MalI GATC 3 cut(s) 422, 554, 709
MbiI CCGCTC 1 cut(s) 70
MboI GATC 3 cut(s) 420, 552, 707
MboII GAAGA 5 cut(s) 59, 139, 163, 765, 768
MhlI GDGCHC 4 cut(s) 204, 223, 588, 645
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 6 cut(s) 38, 118, 131, 167, 272, 661
MluNI TGGCCA 1 cut(s) 428
Mly113I GGCGCC 1 cut(s) 571
Mox20I TGGCCA 1 cut(s) 428
MroNI GCCGGC 1 cut(s) 386
MscI TGGCCA 1 cut(s) 428
MseI TTAA 3 cut(s) 477, 660, 790
Msp20I TGGCCA 1 cut(s) 428
MspI CCGG 4 cut(s) 99, 387, 581, 726
MspR9I CCNGG 3 cut(s) 14, 100, 582
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 2 cut(s) 410, 605
MwoI GCNNNNNNNGC 5 cut(s) 76, 469, 532, 577, 592
NaeI GCCGGC 1 cut(s) 388
NarI GGCGCC 1 cut(s) 571
NciI CCSGG 2 cut(s) 100, 582
NcoI CCATGG 1 cut(s) 712
NdeII GATC 3 cut(s) 420, 552, 707
NgoMIV GCCGGC 1 cut(s) 386
NlaIII CATG 5 cut(s) 127, 427, 617, 670, 716
NlaIV GGNNCC 5 cut(s) 391, 572, 642, 689, 690
NmuCI GTSAC 2 cut(s) 32, 607
PaeR7I CTCGAG 2 cut(s) 182, 308
PciSI GCTCTTC 1 cut(s) 42
PcsI WCGNNNNNNNCGW 3 cut(s) 264, 315, 390
PdiI GCCGGC 1 cut(s) 388
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 8 cut(s) 78, 165, 248, 300, 414, 534, 597, 600
PluTI GGCGCC 1 cut(s) 574
PmaCI CACGTG 1 cut(s) 637
PmlI CACGTG 1 cut(s) 637
Ppu21I YACGTR 1 cut(s) 637
PpuMI RGGWCCY 2 cut(s) 520, 688
Psp5II RGGWCCY 2 cut(s) 520, 688
Psp6I CCWGG 1 cut(s) 12
PspCI CACGTG 1 cut(s) 637
PspFI CCCAGC 1 cut(s) 684
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 5 cut(s) 391, 572, 642, 689, 690
PspPI GGNCC 3 cut(s) 304, 520, 688
PspPPI RGGWCCY 2 cut(s) 520, 688
PspXI VCTCGAGB 2 cut(s) 182, 308
PstI CTGCAG 1 cut(s) 417
PstNI CAGNNNCTG 1 cut(s) 366
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 3 cut(s) 477, 660, 790
SatI GCNGC 8 cut(s) 77, 164, 247, 299, 413, 533, 596, 599
Sau3AI GATC 3 cut(s) 420, 552, 707
Sau96I GGNCC 3 cut(s) 304, 520, 688
ScrFI CCNGG 3 cut(s) 14, 100, 582
SduI GDGCHC 4 cut(s) 204, 223, 588, 645
SetI ASST 9 cut(s) 309, 315, 342, 465, 522, 534, 621, 639, 735
SfcI CTRYAG 1 cut(s) 413
SfoI GGCGCC 1 cut(s) 572
Sfr274I CTCGAG 2 cut(s) 182, 308
SinI GGWCC 3 cut(s) 304, 520, 688
SlaI CTCGAG 2 cut(s) 182, 308
SmlI CTYRAG 3 cut(s) 182, 308, 737
SmoI CTYRAG 3 cut(s) 182, 308, 737
Sse9I AATT 6 cut(s) 38, 118, 131, 167, 272, 661
SspDI GGCGCC 1 cut(s) 570
StyD4I CCNGG 3 cut(s) 12, 98, 580
StyI CCWWGG 1 cut(s) 712
TaiI ACGT 2 cut(s) 621, 639
TaqI TCGA 5 cut(s) 66, 183, 217, 309, 766
TasI AATT 6 cut(s) 38, 118, 131, 167, 272, 661
TauI GCSGC 2 cut(s) 166, 601
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 3 cut(s) 477, 660, 790
Tru9I TTAA 3 cut(s) 477, 660, 790
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 2 cut(s) 32, 607
TseI GCWGC 6 cut(s) 76, 246, 298, 412, 532, 595
Tsp45I GTSAC 2 cut(s) 32, 607
TspDTI ATGAA 3 cut(s) 112, 285, 655
TspGWI ACGGA 1 cut(s) 512
TspRI CASTG 1 cut(s) 422
VpaK11BI GGWCC 3 cut(s) 304, 520, 688
XapI RAATTY 1 cut(s) 272
XhoI CTCGAG 2 cut(s) 182, 308
XmiI GTMKAC 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.