pycom01g21350

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000853
Physical Location & Seq
Forward (+)
19438508 .. 19440547
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21350.7

Sequence Viewer

Length: 1647 bp
ATGATGAGGTCTCCACTCTACCTAACTAAACATCATCAAAGCTTTTTTAGCCGAAAGTTTGAATCCATGATGCTTCCAACTCTTAGGAACCCTACTTCCAACCCTACTTCAAATAAACAACCATCCAATACTACCAGTAACCAGCACACAATCGCCTCCGCCGGATACGGCACTACGTGGCGCCTCCTCCGCCGCAACCTCACCACCGAAATCCTCCACCCTTCCCGCGTCAAATCCTACGGCAAAGCCCGCAAATGGGTTCTCGACATCCTCGTCAACCGCCTTAAAACCAAGTCTCAATCCCACTCCGAAGGTGGCGTCGTCGGTAGTGTTAGGGTGGTCGACCACTTCCAATATGCCCTGTTCTGCCTCCTCGTTCTAATGTGCTTCGGCGACAAATTGAACGAAGAGCAAATCAAAGAAATCGAGCGGGTGCAGCGCCAACAACATTTGAGTTTCGGGCGGTTCAACATCCTCAATTTCAAGCCGAAATTGACGAAGATTTTCCTAAAAAATCGGTGGCGGGAATTCTTCAAAATCCTCGAGGAACAACGAAAAGTACTCGTCCCTCTGATTCGAGCACGACAAAACAAGGCAAAGCAGGGCAGCAAAAACGACGACAAAGACGATGAATTGGTGTTGTCGTATACTGATACGCTGCTGAACCTCGAGCTTCCCGACGGCAAACAGAAGCGGAAGCTTTCGGAGGACGAAATGGTCAACCTCTGCTCGGAGTTTCTCAACGCCGGCACCGACACTACTTCCACCGTGCTGCAGTGGGTCATGGCTAACGTAGTAAAGTACCCGCAAGTTCAGGAGAAGCTGTTTTCAGAGATTAAAGGGGTTATGGGAGAAACGAAGGAGGAGGTGCGAGAGGAGGTCCTGCACAAGCGCCACCCGCCGAGGCACGTTTTGTTTCCGCACTCGGTGACGCATGACGTGGTTTTAGGCGGACACCTGGTGCCCAAAAACGGGACCGTCAATGTCATGGTGGCGGATATAGGGTGGGACGCGCAACTGTGGGAGAACCCCATGGCATTCAAGCCGGAGATGTTCTTGGGCAGCAGCGGCGGAGAAGAAGGGTTCGATTTGACGGGAAGCAGGGAGATTAAGATGATGCCGTTTGGGGTGGGGAGGAGGTCCTGTCCTGGGTCGGGGTTGGCGGTTTTCAGTGGAGGGCTGTGGAGGGAGATGACGTTGACCTATCAGAGAAGATGGACCCAAGAATTTAATGCGTGTGAGAATTTTGGCATAATTCTTCGACGTAACGTTCTCTTTAAAAAGGTAAGCATGAGAGCATCAAGATTCACTTTACGTTTACAAGCACAGCTCAGAGATTACGAAATAGCTTGGGTTGATTCTGAGGCAGGAAAGACAGAAAATGAAATGCTATCTTTACAACCCCTCTCAACCCCGCCTCCACCGCATGAATCTGTCCTTGTTGGTGACTTAAAAATGGCTAATTTCAAGCAATTTCTTTCCGACGAGGGTGTTCAGCTACTTTTTAAGATTGTACAACCCTCCTTTAATCTAGGCGTCACAACTTGGCTGATGACCAATTCGAAATCCAAGTTACTGTGGTCTGAACAAATTCGGTGGAGTAGTGTTTTTGTTTTTACCTTCGAATGTATCGAAGTAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

549

Amino Acids

63.07

Weight (kDa)

9.36

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 272, 716
AbsI CCTCGAGG 1 cut(s) 542
AccB1I GGYRCC 3 cut(s) 180, 749, 961
AccBSI CCGCTC 1 cut(s) 430
AccI GTMKAC 2 cut(s) 342, 647
AccII CGCG 2 cut(s) 228, 1013
AclI AACGTT 1 cut(s) 1269
AcsI RAATTY 4 cut(s) 527, 1226, 1243, 1590
AcyI GRCGYC 3 cut(s) 181, 318, 1536
AdeI CACNNNGTG 3 cut(s) 177, 928, 961
AfaI GTAC 3 cut(s) 561, 803, 1515
AfiI CCNNNNNNNGG 7 cut(s) 255, 256, 730, 971, 972, 1149, 1154
AgsI TTSAA 8 cut(s) 62, 111, 403, 469, 484, 535, 1042, 1468
AjiI CACGTC 1 cut(s) 940
AjnI CCWGG 2 cut(s) 957, 1147
AloI GAACNNNNNNTCC 2 cut(s) 80, 112
AluBI AGCT 7 cut(s) 42, 673, 700, 823, 1330, 1349, 1498
AluI AGCT 7 cut(s) 42, 673, 700, 823, 1330, 1349, 1498
Alw21I GWGCWC 1 cut(s) 583
Alw26I GTCTC 2 cut(s) 15, 300
Ama87I CYCGRG 2 cut(s) 542, 668
ApeKI GCWGC 6 cut(s) 436, 606, 658, 772, 1062, 1065
ApoI RAATTY 4 cut(s) 527, 1226, 1243, 1590
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
Asp700I GAANNNNTTC 2 cut(s) 503, 1590
AspLEI GCGC 4 cut(s) 183, 441, 894, 1015
AspS9I GGNCC 4 cut(s) 880, 975, 1140, 1218
AsuHPI GGTGA 3 cut(s) 193, 940, 1457
AsuII TTCGAA 2 cut(s) 1562, 1623
AvaI CYCGRG 2 cut(s) 542, 668
AvaII GGWCC 4 cut(s) 880, 975, 1140, 1218
BaeGI GKGCMC 1 cut(s) 966
BanI GGYRCC 3 cut(s) 180, 749, 961
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 6 cut(s) 448, 618, 645, 759, 1074, 1077
BccI CCATC 2 cut(s) 130, 1209
BceAI ACGGC 4 cut(s) 184, 256, 697, 1105
BciT130I CCWGG 2 cut(s) 959, 1149
BciVI GTATCC 1 cut(s) 158
BcoDI GTCTC 2 cut(s) 15, 300
BfaI CTAG 1 cut(s) 1532
BfmI CTRYAG 1 cut(s) 773
BfoI RGCGCY 3 cut(s) 184, 442, 895
BfuI GTATCC 1 cut(s) 158
BisI GCNGC 8 cut(s) 193, 437, 607, 659, 773, 1063, 1066, 1069
BlsI GCNGC 8 cut(s) 194, 438, 608, 660, 774, 1064, 1067, 1070
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 2 cut(s) 959, 1149
Bme18I GGWCC 4 cut(s) 880, 975, 1140, 1218
BmeT110I CYCGRG 2 cut(s) 542, 668
BmgBI CACGTC 1 cut(s) 940
BmgT120I GGNCC 4 cut(s) 880, 975, 1140, 1218
BmiI GGNNCC 6 cut(s) 89, 182, 751, 963, 976, 1220
BmrFI CCNGG 2 cut(s) 959, 1149
BmsI GCATC 3 cut(s) 60, 1107, 1307
Bpu14I TTCGAA 2 cut(s) 1562, 1623
BsaAI YACGTR 1 cut(s) 177
BsaHI GRCGYC 3 cut(s) 181, 318, 1536
BsaI GGTCTC 1 cut(s) 15
BsaJI CCNNGG 3 cut(s) 902, 1032, 1148
BsaXI ACNNNNNCTCC 8 cut(s) 809, 839, 1129, 1159, 1181, 1211, 1402, 1432
Bsc4I CCNNNNNNNGG 7 cut(s) 255, 256, 730, 971, 972, 1149, 1154
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 1 cut(s) 135
BseBI CCWGG 2 cut(s) 959, 1149
BseDI CCNNGG 3 cut(s) 902, 1032, 1148
BseGI GGATG 3 cut(s) 122, 267, 471
BseLI CCNNNNNNNGG 7 cut(s) 255, 256, 730, 971, 972, 1149, 1154
BseMII CTCAG 2 cut(s) 1345, 1353
BseNI ACTGG 1 cut(s) 135
BseRI GAGGAG 5 cut(s) 176, 362, 878, 890, 1150
BseSI GKGCMC 1 cut(s) 966
BseXI GCAGC 6 cut(s) 448, 618, 645, 759, 1074, 1077
BsgI GTGCAG 2 cut(s) 455, 869
Bsh1236I CGCG 2 cut(s) 228, 1013
BshNI GGYRCC 3 cut(s) 180, 749, 961
BsiHKAI GWGCWC 1 cut(s) 583
BsiHKCI CYCGRG 2 cut(s) 542, 668
BsiSI CCGG 3 cut(s) 162, 747, 1046
BslFI GGGAC 3 cut(s) 551, 988, 1022
BslI CCNNNNNNNGG 7 cut(s) 255, 256, 730, 971, 972, 1149, 1154
BsmAI GTCTC 2 cut(s) 15, 300
BsmFI GGGAC 3 cut(s) 551, 988, 1022
BsmI GAATGC 1 cut(s) 1037
Bso31I GGTCTC 1 cut(s) 15
BsoBI CYCGRG 2 cut(s) 542, 668
Bsp119I TTCGAA 2 cut(s) 1562, 1623
Bsp1286I GDGCHC 2 cut(s) 583, 966
Bsp1407I TGTACA 1 cut(s) 1513
Bsp19I CCATGG 1 cut(s) 1032
BspCNI CTCAG 2 cut(s) 1344, 1354
BspFNI CGCG 2 cut(s) 228, 1013
BspLI GGNNCC 6 cut(s) 89, 182, 751, 963, 976, 1220
BspMAI CTGCAG 1 cut(s) 777
BspQI GCTCTTC 1 cut(s) 402
BspT104I TTCGAA 2 cut(s) 1562, 1623
BspT107I GGYRCC 3 cut(s) 180, 749, 961
BspTNI GGTCTC 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 746
BsrGI TGTACA 1 cut(s) 1513
BsrI ACTGG 1 cut(s) 135
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 3 cut(s) 902, 1032, 1148
BssNAI GTATAC 1 cut(s) 648
BssNI GRCGYC 3 cut(s) 181, 318, 1536
BssT1I CCWWGG 1 cut(s) 1032
Bst1107I GTATAC 1 cut(s) 648
Bst2UI CCWGG 2 cut(s) 959, 1149
Bst4CI ACNGT 4 cut(s) 769, 979, 1020, 1578
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 3 cut(s) 181, 318, 1536
BstAUI TGTACA 1 cut(s) 1513
BstBAI YACGTR 1 cut(s) 177
BstBI TTCGAA 2 cut(s) 1562, 1623
BstC8I GCNNGC 2 cut(s) 250, 748
BstDEI CTNAG 3 cut(s) 83, 1331, 1362
BstDSI CCRYGG 1 cut(s) 1032
BstF5I GGATG 3 cut(s) 122, 267, 471
BstFNI CGCG 2 cut(s) 228, 1013
BstH2I RGCGCY 3 cut(s) 184, 442, 895
BstHHI GCGC 4 cut(s) 183, 441, 894, 1015
BstMAI GTCTC 2 cut(s) 15, 300
BstMWI GCNNNNNNNGC 7 cut(s) 48, 189, 249, 436, 898, 1068, 1423
BstNI CCWGG 2 cut(s) 959, 1149
BstSCI CCNGG 2 cut(s) 957, 1147
BstSFI CTRYAG 1 cut(s) 773
BstSLI GKGCMC 1 cut(s) 966
BstUI CGCG 2 cut(s) 228, 1013
BstV1I GCAGC 6 cut(s) 448, 618, 645, 759, 1074, 1077
BstZ17I GTATAC 1 cut(s) 648
BsuI GTATCC 1 cut(s) 158
BtgI CCRYGG 1 cut(s) 1032
BtrI CACGTC 1 cut(s) 940
BtsCI GGATG 3 cut(s) 122, 267, 471
BtsI GCAGTG 1 cut(s) 782
BtsIMutI CAGTG 2 cut(s) 782, 1177
Cac8I GCNNGC 2 cut(s) 250, 748
CfoI GCGC 4 cut(s) 183, 441, 894, 1015
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 4 cut(s) 880, 975, 1140, 1218
CseI GACGC 5 cut(s) 217, 307, 940, 1019, 1525
CsiI ACCWGGT 1 cut(s) 957
Csp6I GTAC 3 cut(s) 560, 802, 1514
CspCI CAANNNNNGTGG 2 cut(s) 1577, 1612
CviAII CATG 7 cut(s) 67, 784, 935, 988, 1033, 1291, 1427
CviQI GTAC 3 cut(s) 560, 802, 1514
DdeI CTNAG 3 cut(s) 83, 1331, 1362
DinI GGCGCC 1 cut(s) 182
DraI TTTAAA 1 cut(s) 1279
DraIII CACNNNGTG 3 cut(s) 177, 928, 961
DrdI GACNNNNNNGTC 2 cut(s) 272, 716
DseDI GACNNNNNNGTC 2 cut(s) 272, 716
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 5 cut(s) 148, 179, 966, 1010, 1086
Eco130I CCWWGG 1 cut(s) 1032
Eco31I GGTCTC 1 cut(s) 15
Eco47I GGWCC 4 cut(s) 880, 975, 1140, 1218
Eco88I CYCGRG 2 cut(s) 542, 668
EcoO109I RGGNCCY 2 cut(s) 880, 1140
EcoRI GAATTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 957, 1147
EcoT14I CCWWGG 1 cut(s) 1032
EgeI GGCGCC 1 cut(s) 182
EheI GGCGCC 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 1032
FaeI CATG 7 cut(s) 70, 787, 938, 991, 1036, 1294, 1430
FalI AAGNNNNNCTT 2 cut(s) 1294, 1326
FaqI GGGAC 3 cut(s) 551, 988, 1022
FatI CATG 7 cut(s) 66, 783, 934, 987, 1032, 1290, 1426
FauI CCCGC 7 cut(s) 233, 257, 423, 516, 813, 906, 1422
FblI GTMKAC 2 cut(s) 342, 647
Fnu4HI GCNGC 8 cut(s) 193, 437, 607, 659, 773, 1063, 1066, 1069
FokI GGATG 3 cut(s) 109, 254, 458
Fsp4HI GCNGC 8 cut(s) 193, 437, 607, 659, 773, 1063, 1066, 1069
FspBI CTAG 1 cut(s) 1532
GlaI GCGC 4 cut(s) 182, 440, 893, 1014
GluI GCNGC 8 cut(s) 193, 437, 607, 659, 773, 1063, 1066, 1069
HaeII RGCGCY 3 cut(s) 184, 442, 895
HapII CCGG 3 cut(s) 162, 747, 1046
HgaI GACGC 5 cut(s) 217, 307, 940, 1019, 1525
HhaI GCGC 4 cut(s) 183, 441, 894, 1015
Hin1I GRCGYC 3 cut(s) 181, 318, 1536
Hin1II CATG 7 cut(s) 70, 787, 938, 991, 1036, 1294, 1430
Hin6I GCGC 4 cut(s) 181, 439, 892, 1013
HinP1I GCGC 4 cut(s) 181, 439, 892, 1013
HincII GTYRAC 4 cut(s) 277, 343, 721, 1200
HindII GTYRAC 4 cut(s) 277, 343, 721, 1200
HindIII AAGCTT 2 cut(s) 40, 698
HinfI GANTC 5 cut(s) 62, 574, 1305, 1358, 1430
HpaII CCGG 3 cut(s) 162, 747, 1046
HphI GGTGA 3 cut(s) 193, 940, 1457
Hpy166II GTNNAC 6 cut(s) 277, 343, 648, 721, 1200, 1319
Hpy188III TCNNGA 4 cut(s) 263, 677, 815, 1302
Hpy8I GTNNAC 6 cut(s) 277, 343, 648, 721, 1200, 1319
Hpy99I CGWCG 6 cut(s) 323, 326, 620, 683, 1266, 1487
HpyAV CCTTC 5 cut(s) 231, 305, 853, 1073, 1630
HpyCH4III ACNGT 4 cut(s) 769, 979, 1020, 1578
HpyCH4IV ACGT 8 cut(s) 176, 792, 909, 939, 1196, 1264, 1269, 1315
HpyCH4V TGCA 3 cut(s) 436, 775, 886
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 189, 249, 436, 898, 1068, 1423
HpyF3I CTNAG 3 cut(s) 83, 1331, 1362
HpySE526I ACGT 8 cut(s) 176, 792, 909, 939, 1196, 1264, 1269, 1315
Hsp92I GRCGYC 3 cut(s) 181, 318, 1536
Hsp92II CATG 7 cut(s) 70, 787, 938, 991, 1036, 1294, 1430
HspAI GCGC 4 cut(s) 181, 439, 892, 1013
KasI GGCGCC 1 cut(s) 180
KroI GCCGGC 1 cut(s) 746
KroNI GCCGGC 1 cut(s) 748
LguI GCTCTTC 1 cut(s) 402
Lsp1109I GCAGC 6 cut(s) 448, 618, 645, 759, 1074, 1077
LweI GCATC 3 cut(s) 60, 1107, 1307
MabI ACCWGGT 1 cut(s) 957
MaeI CTAG 1 cut(s) 1532
MaeII ACGT 8 cut(s) 176, 792, 909, 939, 1196, 1264, 1269, 1315
MaeIII GTNAC 6 cut(s) 137, 928, 1265, 1445, 1537, 1572
MbiI CCGCTC 1 cut(s) 430
MboII GAAGA 6 cut(s) 419, 511, 523, 1088, 1224, 1250
MhlI GDGCHC 2 cut(s) 583, 966
Mly113I GGCGCC 1 cut(s) 181
MmeI TCCRAC 3 cut(s) 101, 123, 1506
MroNI GCCGGC 1 cut(s) 746
MroXI GAANNNNTTC 2 cut(s) 503, 1590
MseI TTAA 8 cut(s) 285, 837, 1110, 1230, 1278, 1451, 1506, 1527
MspA1I CMGCKG 1 cut(s) 1068
MspI CCGG 3 cut(s) 162, 747, 1046
MspR9I CCNGG 2 cut(s) 959, 1149
Mva1269I GAATGC 1 cut(s) 1037
MvaI CCWGG 2 cut(s) 959, 1149
MvnI CGCG 2 cut(s) 228, 1013
MwoI GCNNNNNNNGC 7 cut(s) 48, 189, 249, 436, 898, 1068, 1423
NaeI GCCGGC 1 cut(s) 748
NarI GGCGCC 1 cut(s) 181
NcoI CCATGG 1 cut(s) 1032
NgoMIV GCCGGC 1 cut(s) 746
NlaIII CATG 7 cut(s) 70, 787, 938, 991, 1036, 1294, 1430
NlaIV GGNNCC 6 cut(s) 89, 182, 751, 963, 976, 1220
NmeAIII GCCGAG 1 cut(s) 927
NmuCI GTSAC 3 cut(s) 928, 1445, 1537
NspV TTCGAA 2 cut(s) 1562, 1623
PaeR7I CTCGAG 2 cut(s) 542, 668
PciSI GCTCTTC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 5 cut(s) 270, 624, 675, 750, 1629
PctI GAATGC 1 cut(s) 1037
PdiI GCCGGC 1 cut(s) 748
PdmI GAANNNNTTC 2 cut(s) 503, 1590
PfeI GAWTC 5 cut(s) 62, 574, 1305, 1358, 1430
PkrI GCNGC 8 cut(s) 194, 438, 608, 660, 774, 1064, 1067, 1070
PluTI GGCGCC 1 cut(s) 184
Ppu21I YACGTR 1 cut(s) 177
PpuMI RGGWCCY 2 cut(s) 880, 1140
Psp1406I AACGTT 1 cut(s) 1269
Psp5II RGGWCCY 2 cut(s) 880, 1140
Psp6I CCWGG 2 cut(s) 957, 1147
PspGI CCWGG 2 cut(s) 957, 1147
PspN4I GGNNCC 6 cut(s) 89, 182, 751, 963, 976, 1220
PspPI GGNCC 4 cut(s) 880, 975, 1140, 1218
PspPPI RGGWCCY 2 cut(s) 880, 1140
PspXI VCTCGAGB 2 cut(s) 542, 668
PstI CTGCAG 1 cut(s) 777
RsaI GTAC 3 cut(s) 561, 803, 1515
RsaNI GTAC 3 cut(s) 560, 802, 1514
SalI GTCGAC 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 402
SaqAI TTAA 8 cut(s) 285, 837, 1110, 1230, 1278, 1451, 1506, 1527
SatI GCNGC 8 cut(s) 193, 437, 607, 659, 773, 1063, 1066, 1069
Sau96I GGNCC 4 cut(s) 880, 975, 1140, 1218
ScaI AGTACT 1 cut(s) 561
ScrFI CCNGG 2 cut(s) 959, 1149
SduI GDGCHC 2 cut(s) 583, 966
SexAI ACCWGGT 1 cut(s) 957
SfaNI GCATC 3 cut(s) 60, 1107, 1307
SfcI CTRYAG 1 cut(s) 773
SfoI GGCGCC 1 cut(s) 182
Sfr274I CTCGAG 2 cut(s) 542, 668
SfuI TTCGAA 2 cut(s) 1562, 1623
SinI GGWCC 4 cut(s) 880, 975, 1140, 1218
SlaI CTCGAG 2 cut(s) 542, 668
SmlI CTYRAG 2 cut(s) 542, 668
SmoI CTYRAG 2 cut(s) 542, 668
SspDI GGCGCC 1 cut(s) 180
SspMI CTAG 1 cut(s) 1532
StyD4I CCNGG 2 cut(s) 957, 1147
StyI CCWWGG 1 cut(s) 1032
TaaI ACNGT 4 cut(s) 769, 979, 1020, 1578
TaiI ACGT 8 cut(s) 179, 795, 912, 942, 1199, 1267, 1272, 1318
TatI WGTACW 2 cut(s) 559, 1513
TauI GCSGC 2 cut(s) 195, 1071
TfiI GAWTC 5 cut(s) 62, 574, 1305, 1358, 1430
Tru1I TTAA 8 cut(s) 285, 837, 1110, 1230, 1278, 1451, 1506, 1527
Tru9I TTAA 8 cut(s) 285, 837, 1110, 1230, 1278, 1451, 1506, 1527
TscAI CASTG 2 cut(s) 782, 1177
TseFI GTSAC 3 cut(s) 928, 1445, 1537
TseI GCWGC 6 cut(s) 436, 606, 658, 772, 1062, 1065
Tsp45I GTSAC 3 cut(s) 928, 1445, 1537
TspDTI ATGAA 3 cut(s) 645, 1398, 1443
TspRI CASTG 2 cut(s) 782, 1177
VpaK11BI GGWCC 4 cut(s) 880, 975, 1140, 1218
XapI RAATTY 4 cut(s) 527, 1226, 1243, 1590
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XhoI CTCGAG 2 cut(s) 542, 668
XmiI GTMKAC 2 cut(s) 342, 647
XmnI GAANNNNTTC 2 cut(s) 503, 1590
XspI CTAG 1 cut(s) 1532
ZrmI AGTACT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.