Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Reverse (-)

1064582 .. 1071499

Gene Structure 6918 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g01650.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGACAACCCTAACAAAACTATGGCTGATCACCATCCTCTTCTTCCTTCCTTGCTTTCTCCGCGACGTCTTCGGAGTCCAGATCCTCTCCAAATCAAAACTCGAGAAGTGCGAGAAGACTTCCGATTCCGGTGACCTCAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGTGGCGCGAGTGGGGGTGAGGCTTCGATCGTTGTGGAAATTGTAGAGGTGGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCGCCTGTCGTGACTGTTAATAAATCCGCAGCTTATGCCTTGTATGAGCTTACGTACATTCGAGATGTTGCATACAAGCCAGAAGAGTATCATGTTGAGACTCGAAAGTGTGAGCCGGATGCAGATGCCAAAGTGGTGAAGATATGTTACGAGATGAAAATGGTCACATCATCGAGCACACAGAGCCAATGTGCTGTCCTTGCGGGACTCAGCGGCGAGTCCCTTCATCATGTGGAAATGTTTTTGATGAAAGGAAAGGCCAATACAGCTCACTGTGTCCGCTTTCCGGGTGATTGGTTTCATGTTTTTGGTATTGGACAGCGGTCAGTGGGATTCAGTGTTCTCATTCAAGTAAAGACAGGATCTAAAGTTTCGGAAGTGCTTGTAGGTCCTGAAAACAGAACAGCAATATCCAATGACAATTTTTTGCGAGCTAATCTTATTGGAGATTTTGTTGGATACACAAGTATACCGTCATTTGAGGACTTTTACCTTGTAATTCCAAGGCAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCGACTTTGAAGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAGTTATGGAATTTCTGGGAGGCTGATCGAAACCGTGTCCAAAGGAAACAGGTGCCGCTTTTTGGTGTGGAAGGGAGGTTTGAAAGGATAAACCAGCATCCAGTAATGTATCAGAAATATTTGAGTTACTCTTCTGAAGGAATTTCTATCATTTGGATACATATAAACTCTGATTTGGTGCAGAATGCAGGAACTCATTCGTTTTCCATTGGAGTCACGGAAATCATTAATACAAATCTCTTGATTGAACTAAGTGCTGATGATGTAGAATATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCCACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAGAATACTGGTGAAGTGGAAGCATCTTATTGTTTAACGTTTGATTGCTCAAGTGATGTCACCCTTATGGAGGAACAATACTTCATTATGAAGCCAAAAGAAACGTTAACTCGATCCTTCAAACTTTACCCAGGAACTGATCAAGCTGCAAAATACGTCTGTGCAGCTATACTCAAGGATTCTAATTATAATGAAGTTGATAGAGCTGAGTGCCAATTTTCTACTACGTCTACTGTCATTGATAATGGATCACAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

505

Amino Acids

56.54

Weight (kDa)

5.62

Isoelectric Point (pI)

32.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	46 - 503	1.9e-123	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1443
AatII	GACGTC	1 cut(s)	69
AccB1I	GGYRCC	1 cut(s)	943
AccI	GTMKAC	4 cut(s)	736, 825, 1167, 1484
AccII	CGCG	2 cut(s)	63, 185
AciI	CCGC	8 cut(s)	61, 269, 294, 470, 480, 547, 589, 947
AclI	AACGTT	2 cut(s)	1292, 1358
AclWI	GGATC	4 cut(s)	76, 637, 1362, 1510
AcoI	YGGCCR	1 cut(s)	168
AcsI	RAATTY	3 cut(s)	235, 901, 1032
AcuI	CTGAAG	1 cut(s)	1047
AcyI	GRCGYC	1 cut(s)	66
AfaI	GTAC	1 cut(s)	323
AfiI	CCNNNNNNNGG	4 cut(s)	166, 553, 953, 1324
AflII	CTTAAG	1 cut(s)	1192
AgsI	TTSAA	7 cut(s)	240, 617, 806, 833, 974, 1139, 1375
AjnI	CCWGG	2 cut(s)	1180, 1384
AloI	GAACNNNNNNTCC	2 cut(s)	591, 623
AluBI	AGCT	8 cut(s)	299, 316, 536, 701, 836, 1400, 1421, 1460
AluI	AGCT	8 cut(s)	299, 316, 536, 701, 836, 1400, 1421, 1460
Alw21I	GWGCWC	1 cut(s)	446
Alw26I	GTCTC	1 cut(s)	359
AlwI	GGATC	4 cut(s)	76, 637, 1362, 1510
AlwNI	CAGNNNCTG	1 cut(s)	1391
Ama87I	CYCGRG	1 cut(s)	101
AoxI	GGCC	3 cut(s)	168, 525, 1218
ApeKI	GCWGC	4 cut(s)	296, 847, 1400, 1418
ApoI	RAATTY	3 cut(s)	235, 901, 1032
AseI	ATTAAT	1 cut(s)	1119
Asp700I	GAANNNNTTC	1 cut(s)	1087
AspLEI	GCGC	1 cut(s)	185
AspS9I	GGNCC	2 cut(s)	656, 1219
AsuC2I	CCSGG	1 cut(s)	555
AsuHPI	GGTGA	8 cut(s)	22, 143, 206, 415, 569, 858, 1277, 1306
AsuII	TTCGAA	1 cut(s)	262
AvaI	CYCGRG	1 cut(s)	101
AvaII	GGWCC	1 cut(s)	656
BaeI	ACNNNNGTAYC	2 cut(s)	718, 751
BanI	GGYRCC	1 cut(s)	943
BarI	GAAGNNNNNNTAC	2 cut(s)	398, 430
BbsI	GAAGAC	2 cut(s)	61, 122
Bbv12I	GWGCWC	1 cut(s)	446
BbvI	GCAGC	4 cut(s)	308, 859, 1387, 1430
BccI	CCATC	2 cut(s)	41, 791
BceAI	ACGGC	1 cut(s)	155
BcgI	CGANNNNNNTGC	2 cut(s)	242, 276
BciT130I	CCWGG	2 cut(s)	1182, 1386
BciVI	GTATCC	2 cut(s)	719, 1041
BclI	TGATCA	2 cut(s)	27, 1393
BcnI	CCSGG	1 cut(s)	555
BcoDI	GTCTC	1 cut(s)	359
BfaI	CTAG	1 cut(s)	177
BfrI	CTTAAG	1 cut(s)	1192
BfuI	GTATCC	2 cut(s)	719, 1041
BisI	GCNGC	6 cut(s)	297, 481, 848, 947, 1401, 1419
BlsI	GCNGC	6 cut(s)	298, 482, 849, 948, 1402, 1420
Bme1390I	CCNGG	3 cut(s)	555, 1182, 1386
Bme18I	GGWCC	1 cut(s)	656
BmeT110I	CYCGRG	1 cut(s)	101
BmgT120I	GGNCC	2 cut(s)	656, 1219
BmiI	GGNNCC	1 cut(s)	945
BmrFI	CCNGG	3 cut(s)	555, 1182, 1386
BmsI	GCATC	6 cut(s)	240, 376, 382, 997, 1206, 1286
BoxI	GACNNNNGTC	1 cut(s)	589
BpiI	GAAGAC	2 cut(s)	61, 122
BpmI	CTGGAG	1 cut(s)	895
Bpu14I	TTCGAA	1 cut(s)	262
BpuEI	CTTGAG	2 cut(s)	1288, 1412
BpuMI	CCSGG	1 cut(s)	555
BsaAI	YACGTR	1 cut(s)	321
BsaBI	GATNNNNATC	1 cut(s)	32
BsaHI	GRCGYC	1 cut(s)	66
BsaJI	CCNNGG	3 cut(s)	770, 1181, 1384
BsaWI	WCCGGW	1 cut(s)	128
BsaXI	ACNNNNNCTCC	2 cut(s)	705, 735
Bsc4I	CCNNNNNNNGG	4 cut(s)	166, 553, 953, 1324
Bse1I	ACTGG	2 cut(s)	992, 1267
Bse8I	GATNNNNATC	1 cut(s)	32
BseBI	CCWGG	2 cut(s)	1182, 1386
BseDI	CCNNGG	3 cut(s)	770, 1181, 1384
BseGI	GGATG	3 cut(s)	33, 391, 988
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	4 cut(s)	166, 553, 953, 1324
BseMII	CTCAG	2 cut(s)	490, 1452
BseNI	ACTGG	2 cut(s)	992, 1267
BseRI	GAGGAG	1 cut(s)	245
BseXI	GCAGC	4 cut(s)	308, 859, 1387, 1430
BsgI	GTGCAG	3 cut(s)	127, 1091, 1437
Bsh1236I	CGCG	2 cut(s)	63, 185
Bsh1285I	CGRYCG	1 cut(s)	207
BshFI	GGCC	3 cut(s)	170, 527, 1220
BshNI	GGYRCC	1 cut(s)	943
BsiEI	CGRYCG	1 cut(s)	207
BsiHKAI	GWGCWC	1 cut(s)	446
BsiHKCI	CYCGRG	1 cut(s)	101
BsiSI	CCGG	3 cut(s)	129, 383, 554
BslFI	GGGAC	3 cut(s)	158, 472, 486
BslI	CCNNNNNNNGG	4 cut(s)	166, 553, 953, 1324
BsmAI	GTCTC	1 cut(s)	359
BsmFI	GGGAC	3 cut(s)	158, 472, 486
BsmI	GAATGC	1 cut(s)	1081
BsnI	GGCC	3 cut(s)	170, 527, 1220
BsoBI	CYCGRG	1 cut(s)	101
Bsp119I	TTCGAA	1 cut(s)	262
Bsp1286I	GDGCHC	1 cut(s)	446
Bsp143I	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
BspACI	CCGC	8 cut(s)	61, 269, 294, 470, 480, 547, 589, 947
BspANI	GGCC	3 cut(s)	170, 527, 1220
BspCNI	CTCAG	2 cut(s)	489, 1453
BspFNI	CGCG	2 cut(s)	63, 185
BspLI	GGNNCC	1 cut(s)	945
BspPI	GGATC	4 cut(s)	76, 637, 1362, 1510
BspQI	GCTCTTC	1 cut(s)	868
BspT104I	TTCGAA	1 cut(s)	262
BspT107I	GGYRCC	1 cut(s)	943
BspTI	CTTAAG	1 cut(s)	1192
BsrI	ACTGG	2 cut(s)	992, 1267
BssECI	CCNNGG	3 cut(s)	770, 1181, 1384
BssMI	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
BssNAI	GTATAC	1 cut(s)	737
BssNI	GRCGYC	1 cut(s)	66
BssT1I	CCWWGG	1 cut(s)	770
Bst1107I	GTATAC	1 cut(s)	737
Bst2UI	CCWGG	2 cut(s)	1182, 1386
Bst4CI	ACNGT	5 cut(s)	283, 542, 741, 926, 1489
Bst6I	CTCTTC	4 cut(s)	44, 345, 868, 1027
BstACI	GRCGYC	1 cut(s)	66
BstAFI	CTTAAG	1 cut(s)	1192
BstAPI	GCANNNNNTGC	1 cut(s)	302
BstBAI	YACGTR	1 cut(s)	321
BstBI	TTCGAA	1 cut(s)	262
BstC8I	GCNNGC	2 cut(s)	699, 884
BstDEI	CTNAG	4 cut(s)	147, 476, 1142, 1461
BstEII	GGTNACC	1 cut(s)	131
BstENI	CCTNNNNNAGG	1 cut(s)	1322
BstF5I	GGATG	3 cut(s)	33, 391, 988
BstFNI	CGCG	2 cut(s)	63, 185
BstHHI	GCGC	1 cut(s)	185
BstKTI	GATC	9 cut(s)	30, 84, 156, 207, 632, 919, 1370, 1396, 1505
BstMAI	GTCTC	1 cut(s)	359
BstMBI	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
BstMCI	CGRYCG	1 cut(s)	207
BstMWI	GCNNNNNNNGC	5 cut(s)	60, 302, 450, 467, 533
BstNI	CCWGG	2 cut(s)	1182, 1386
BstPAI	GACNNNNGTC	1 cut(s)	589
BstPI	GGTNACC	1 cut(s)	131
BstSCI	CCNGG	3 cut(s)	553, 1180, 1384
BstSNI	TACGTA	1 cut(s)	321
BstUI	CGCG	2 cut(s)	63, 185
BstV1I	GCAGC	4 cut(s)	308, 859, 1387, 1430
BstV2I	GAAGAC	2 cut(s)	61, 122
BstX2I	RGATCY	2 cut(s)	81, 629
BstYI	RGATCY	2 cut(s)	81, 629
BstZ17I	GTATAC	1 cut(s)	737
BsuI	GTATCC	2 cut(s)	719, 1041
BsuRI	GGCC	3 cut(s)	170, 527, 1220
BtsCI	GGATG	3 cut(s)	33, 391, 988
BtsIMutI	CAGTG	3 cut(s)	538, 600, 610
Cac8I	GCNNGC	2 cut(s)	699, 884
CaiI	CAGNNNCTG	1 cut(s)	1391
CfoI	GCGC	1 cut(s)	185
Cfr13I	GGNCC	2 cut(s)	656, 1219
Csp6I	GTAC	1 cut(s)	322
CviAII	CATG	4 cut(s)	166, 359, 497, 569
CviQI	GTAC	1 cut(s)	322
DdeI	CTNAG	4 cut(s)	147, 476, 1142, 1461
DpnI	GATC	9 cut(s)	29, 83, 155, 206, 631, 918, 1369, 1395, 1504
DpnII	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
EaeI	YGGCCR	1 cut(s)	168
Eam1104I	CTCTTC	4 cut(s)	44, 345, 868, 1027
EarI	CTCTTC	4 cut(s)	44, 345, 868, 1027
Eco105I	TACGTA	1 cut(s)	321
Eco130I	CCWWGG	1 cut(s)	770
Eco47I	GGWCC	1 cut(s)	656
Eco57I	CTGAAG	1 cut(s)	1047
Eco88I	CYCGRG	1 cut(s)	101
Eco91I	GGTNACC	1 cut(s)	131
EcoNI	CCTNNNNNAGG	1 cut(s)	1322
EcoO109I	RGGNCCY	2 cut(s)	656, 1219
EcoO65I	GGTNACC	1 cut(s)	131
EcoRI	GAATTC	1 cut(s)	235
EcoRII	CCWGG	2 cut(s)	1180, 1384
EcoT14I	CCWWGG	1 cut(s)	770
ErhI	CCWWGG	1 cut(s)	770
FaeI	CATG	4 cut(s)	169, 362, 500, 572
FaqI	GGGAC	3 cut(s)	158, 472, 486
FatI	CATG	4 cut(s)	165, 358, 496, 568
FauI	CCCGC	1 cut(s)	463
FbaI	TGATCA	2 cut(s)	27, 1393
FblI	GTMKAC	4 cut(s)	736, 825, 1167, 1484
Fnu4HI	GCNGC	6 cut(s)	297, 481, 848, 947, 1401, 1419
FokI	GGATG	3 cut(s)	20, 398, 975
Fsp4HI	GCNGC	6 cut(s)	297, 481, 848, 947, 1401, 1419
FspBI	CTAG	1 cut(s)	177
GlaI	GCGC	1 cut(s)	184
GluI	GCNGC	6 cut(s)	297, 481, 848, 947, 1401, 1419
GsuI	CTGGAG	1 cut(s)	895
HaeIII	GGCC	3 cut(s)	170, 527, 1220
HapII	CCGG	3 cut(s)	129, 383, 554
HhaI	GCGC	1 cut(s)	185
Hin1I	GRCGYC	1 cut(s)	66
Hin1II	CATG	4 cut(s)	169, 362, 500, 572
Hin6I	GCGC	1 cut(s)	183
HinP1I	GCGC	1 cut(s)	183
HincII	GTYRAC	2 cut(s)	826, 1362
HindII	GTYRAC	2 cut(s)	826, 1362
HindIII	AAGCTT	1 cut(s)	834
HinfI	GANTC	9 cut(s)	75, 125, 367, 474, 485, 600, 1104, 1177, 1433
HpaI	GTTAAC	1 cut(s)	1362
HpaII	CCGG	3 cut(s)	129, 383, 554
HphI	GGTGA	8 cut(s)	22, 143, 206, 415, 569, 858, 1277, 1306
Hpy166II	GTNNAC	5 cut(s)	737, 826, 1168, 1362, 1485
Hpy188I	TCNGA	6 cut(s)	74, 124, 643, 1005, 1027, 1063
Hpy188III	TCNNGA	7 cut(s)	79, 103, 277, 329, 659, 874, 1132
Hpy8I	GTNNAC	5 cut(s)	737, 826, 1168, 1362, 1485
Hpy99I	CGWCG	1 cut(s)	68
HpyAV	CCTTC	5 cut(s)	56, 500, 956, 1022, 1381
HpyCH4III	ACNGT	5 cut(s)	283, 542, 741, 926, 1489
HpyCH4IV	ACGT	6 cut(s)	66, 320, 1292, 1358, 1410, 1481
HpyCH4V	TGCA	9 cut(s)	144, 253, 338, 389, 886, 1072, 1079, 1403, 1418
HpyF10VI	GCNNNNNNNGC	5 cut(s)	60, 302, 450, 467, 533
HpyF3I	CTNAG	4 cut(s)	147, 476, 1142, 1461
HpySE526I	ACGT	6 cut(s)	66, 320, 1292, 1358, 1410, 1481
Hsp92I	GRCGYC	1 cut(s)	66
Hsp92II	CATG	4 cut(s)	169, 362, 500, 572
HspAI	GCGC	1 cut(s)	183
Ksp22I	TGATCA	2 cut(s)	27, 1393
KspAI	GTTAAC	1 cut(s)	1362
Kzo9I	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
LguI	GCTCTTC	1 cut(s)	868
Lsp1109I	GCAGC	4 cut(s)	308, 859, 1387, 1430
LweI	GCATC	6 cut(s)	240, 376, 382, 997, 1206, 1286
MaeI	CTAG	1 cut(s)	177
MaeII	ACGT	6 cut(s)	66, 320, 1292, 1358, 1410, 1481
MaeIII	GTNAC	7 cut(s)	131, 277, 413, 430, 1016, 1105, 1312
MalI	GATC	9 cut(s)	29, 83, 155, 206, 631, 918, 1369, 1395, 1504
MboI	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
MflI	RGATCY	2 cut(s)	81, 629
MhlI	GDGCHC	1 cut(s)	446
MlyI	GAGTC	6 cut(s)	84, 361, 468, 494, 1113, 1186
MmeI	TCCRAC	1 cut(s)	703
MroXI	GAANNNNTTC	1 cut(s)	1087
MseI	TTAA	6 cut(s)	285, 840, 1119, 1193, 1289, 1361
MslI	CAYNNNNRTG	2 cut(s)	1319, 1510
MspA1I	CMGCKG	2 cut(s)	480, 589
MspCI	CTTAAG	1 cut(s)	1192
MspI	CCGG	3 cut(s)	129, 383, 554
MspR9I	CCNGG	3 cut(s)	555, 1182, 1386
Mva1269I	GAATGC	1 cut(s)	1081
MvaI	CCWGG	2 cut(s)	1182, 1386
MvnI	CGCG	2 cut(s)	63, 185
MwoI	GCNNNNNNNGC	5 cut(s)	60, 302, 450, 467, 533
NciI	CCSGG	1 cut(s)	555
NdeII	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
NlaIII	CATG	4 cut(s)	169, 362, 500, 572
NlaIV	GGNNCC	1 cut(s)	945
NmuCI	GTSAC	5 cut(s)	131, 277, 430, 1105, 1312
NspV	TTCGAA	1 cut(s)	262
PaeR7I	CTCGAG	1 cut(s)	101
PciSI	GCTCTTC	1 cut(s)	868
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PctI	GAATGC	1 cut(s)	1081
PdmI	GAANNNNTTC	1 cut(s)	1087
PfeI	GAWTC	3 cut(s)	125, 600, 1433
PkrI	GCNGC	6 cut(s)	298, 482, 849, 948, 1402, 1420
Ple19I	CGATCG	1 cut(s)	207
PleI	GAGTC	6 cut(s)	83, 361, 468, 493, 1112, 1185
PpsI	GAGTC	6 cut(s)	83, 361, 468, 493, 1112, 1185
Ppu21I	YACGTR	1 cut(s)	321
PpuMI	RGGWCCY	1 cut(s)	656
PshAI	GACNNNNGTC	1 cut(s)	589
PshBI	ATTAAT	1 cut(s)	1119
PsiI	TTATAA	1 cut(s)	1443
Psp1406I	AACGTT	2 cut(s)	1292, 1358
Psp5II	RGGWCCY	1 cut(s)	656
Psp6I	CCWGG	2 cut(s)	1180, 1384
PspEI	GGTNACC	1 cut(s)	131
PspGI	CCWGG	2 cut(s)	1180, 1384
PspN4I	GGNNCC	1 cut(s)	945
PspPI	GGNCC	2 cut(s)	656, 1219
PspPPI	RGGWCCY	1 cut(s)	656
PstNI	CAGNNNCTG	1 cut(s)	1391
PsuI	RGATCY	2 cut(s)	81, 629
PvuI	CGATCG	1 cut(s)	207
RsaI	GTAC	1 cut(s)	323
RsaNI	GTAC	1 cut(s)	322
RseI	CAYNNNNRTG	2 cut(s)	1319, 1510
SalI	GTCGAC	1 cut(s)	824
SapI	GCTCTTC	1 cut(s)	868
SaqAI	TTAA	6 cut(s)	285, 840, 1119, 1193, 1289, 1361
SatI	GCNGC	6 cut(s)	297, 481, 848, 947, 1401, 1419
Sau3AI	GATC	9 cut(s)	27, 81, 153, 204, 629, 916, 1367, 1393, 1502
Sau96I	GGNCC	2 cut(s)	656, 1219
SchI	GAGTC	6 cut(s)	84, 361, 468, 494, 1113, 1186
ScrFI	CCNGG	3 cut(s)	555, 1182, 1386
SduI	GDGCHC	1 cut(s)	446
SfaNI	GCATC	6 cut(s)	240, 376, 382, 997, 1206, 1286
Sfr274I	CTCGAG	1 cut(s)	101
SfuI	TTCGAA	1 cut(s)	262
SinI	GGWCC	1 cut(s)	656
SlaI	CTCGAG	1 cut(s)	101
SmiMI	CAYNNNNRTG	2 cut(s)	1319, 1510
SmlI	CTYRAG	4 cut(s)	101, 1192, 1303, 1427
SmoI	CTYRAG	4 cut(s)	101, 1192, 1303, 1427
SnaBI	TACGTA	1 cut(s)	321
SsiI	CCGC	8 cut(s)	61, 269, 294, 470, 480, 547, 589, 947
SspI	AATATT	1 cut(s)	1010
SspMI	CTAG	1 cut(s)	177
StyD4I	CCNGG	3 cut(s)	553, 1180, 1384
StyI	CCWWGG	1 cut(s)	770
TaaI	ACNGT	5 cut(s)	283, 542, 741, 926, 1489
TaiI	ACGT	6 cut(s)	69, 323, 1295, 1361, 1413, 1484
TaqI	TCGA	9 cut(s)	102, 203, 262, 328, 370, 440, 825, 919, 1366
TauI	GCSGC	2 cut(s)	483, 949
TfiI	GAWTC	3 cut(s)	125, 600, 1433
Tru1I	TTAA	6 cut(s)	285, 840, 1119, 1193, 1289, 1361
Tru9I	TTAA	6 cut(s)	285, 840, 1119, 1193, 1289, 1361
TscAI	CASTG	3 cut(s)	545, 600, 610
TseFI	GTSAC	5 cut(s)	131, 277, 430, 1105, 1312
TseI	GCWGC	4 cut(s)	296, 847, 1400, 1418
Tsp45I	GTSAC	5 cut(s)	131, 277, 430, 1105, 1312
TspDTI	ATGAA	7 cut(s)	437, 482, 530, 557, 1327, 1358, 1461
TspGWI	ACGGA	1 cut(s)	1124
TspRI	CASTG	3 cut(s)	545, 600, 610
Vha464I	CTTAAG	1 cut(s)	1192
VpaK11BI	GGWCC	1 cut(s)	656
VspI	ATTAAT	1 cut(s)	1119
XagI	CCTNNNNNAGG	1 cut(s)	1322
XapI	RAATTY	3 cut(s)	235, 901, 1032
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1178
XhoI	CTCGAG	1 cut(s)	101
XmiI	GTMKAC	4 cut(s)	736, 825, 1167, 1484
XmnI	GAANNNNTTC	1 cut(s)	1087
XspI	CTAG	1 cut(s)	177
ZraI	GACGTC	1 cut(s)	67

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.