Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

34212452 .. 34218062

Gene Structure 5611 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00355510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGGGAAACCCTCCACCAAAATTATGGCTTATCACCCTCCTCTTCCTCCCTTGCTTCTTCATAAGCGACGGCGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTCCGAATCCGGTGACCTTAACTGCACCAAGAAGATAGTTCTCAACATGGCCGTCCCTAGCGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAAATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCGCCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACGACCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTCAAGATATGTTACGAGATGAAAATGGTACCATTATTGAGCATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCATGTGGAAGTGTTTGTAATTGACAAGTTGATGAAAGGAAAACCCAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTGTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGGATCTAAAGTTTCGGAAGTGCTTGTAGGTCCTGAAAACAGAACAGCAATATCTAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGACTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGGGGCCAAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCGTTCTGGAGTTGCTTGCATAATCAGTTATGGAATTTCTTGGAGAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAAGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAGAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGCTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAATGTTAACTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAACGAAGTGGATAGAGCCGAGTGCCAATTTTCTACGACGTCTACTGTCATTGATAATGGATCACAGGTTAATCCCTTTAAGCCACCAAAGACAGGAACAACTGGTTTCTTCGACTCCATTGAAAGCTTTTGGAACCATGCGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGGTACTTGTTGCACAGTGGAATAATCAATTCTGGCTCATTTCTAACTGATATACTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

532

Amino Acids

59.22

Weight (kDa)

5.86

Isoelectric Point (pI)

32.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	48 - 330	7.8e-72	Male gamete fusion factor
HAP2-GCS1	PF10699	329 - 503	5.2e-49	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1359
AatII	GACGTC	1 cut(s)	1400
Acc36I	ACCTGC	1 cut(s)	1530
Acc65I	GGTACC	2 cut(s)	436, 492
AccB1I	GGYRCC	2 cut(s)	436, 492
AccB7I	CCANNNNNTGG	1 cut(s)	820
AccI	GTMKAC	2 cut(s)	1083, 1400
AccII	CGCG	1 cut(s)	191
AciI	CCGC	7 cut(s)	186, 218, 275, 300, 486, 489, 565
AclI	AACGTT	1 cut(s)	1208
AclWI	GGATC	3 cut(s)	82, 655, 1426
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	3 cut(s)	241, 817, 979
AcuI	CTGAAG	1 cut(s)	375
AcyI	GRCGYC	2 cut(s)	72, 1397
AfaI	GTAC	6 cut(s)	317, 329, 362, 438, 494, 1544
AfeI	AGCGCT	1 cut(s)	608
AfiI	CCNNNNNNNGG	5 cut(s)	571, 794, 820, 1240, 1451
AflII	CTTAAG	2 cut(s)	1108, 1343
AgsI	TTSAA	6 cut(s)	246, 635, 884, 1133, 1291, 1481
AjnI	CCWGG	2 cut(s)	1096, 1300
AloI	GAACNNNNNNTCC	2 cut(s)	609, 641
AluBI	AGCT	5 cut(s)	305, 719, 1316, 1337, 1485
AluI	AGCT	5 cut(s)	305, 719, 1316, 1337, 1485
Alw26I	GTCTC	1 cut(s)	726
AlwI	GGATC	3 cut(s)	82, 655, 1426
Aor51HI	AGCGCT	1 cut(s)	608
AoxI	GGCC	3 cut(s)	174, 478, 799
ApeKI	GCWGC	5 cut(s)	302, 847, 925, 1316, 1334
ApoI	RAATTY	3 cut(s)	241, 817, 979
AseI	ATTAAT	1 cut(s)	1035
Asp718I	GGTACC	2 cut(s)	436, 492
AspLEI	GCGC	2 cut(s)	191, 609
AspS9I	GGNCC	3 cut(s)	478, 674, 799
AsuC2I	CCSGG	1 cut(s)	573
AsuHPI	GGTGA	7 cut(s)	25, 149, 212, 587, 936, 1193, 1222
AsuII	TTCGAA	1 cut(s)	268
AvaII	GGWCC	1 cut(s)	674
BanI	GGYRCC	2 cut(s)	436, 492
BbsI	GAAGAC	2 cut(s)	128, 1511
BbvCI	CCTCAGC	1 cut(s)	482
BbvI	GCAGC	5 cut(s)	314, 834, 937, 1303, 1346
BccI	CCATC	1 cut(s)	869
BceAI	ACGGC	2 cut(s)	85, 161
BcgI	CGANNNNNNTGC	4 cut(s)	248, 282, 1298, 1332
BciT130I	CCWGG	2 cut(s)	1098, 1302
BciVI	GTATCC	1 cut(s)	737
BcnI	CCSGG	1 cut(s)	573
BcoDI	GTCTC	1 cut(s)	726
BfaI	CTAG	2 cut(s)	183, 1597
BfoI	RGCGCY	1 cut(s)	610
BfrI	CTTAAG	2 cut(s)	1108, 1343
BfuAI	ACCTGC	1 cut(s)	1530
BfuI	GTATCC	1 cut(s)	737
BglI	GCCNNNNNGGC	1 cut(s)	486
BisI	GCNGC	7 cut(s)	187, 303, 487, 848, 926, 1317, 1335
BlsI	GCNGC	7 cut(s)	188, 304, 488, 849, 927, 1318, 1336
BmcAI	AGTACT	1 cut(s)	362
Bme1390I	CCNGG	3 cut(s)	573, 1098, 1302
Bme18I	GGWCC	1 cut(s)	674
BmgT120I	GGNCC	3 cut(s)	478, 674, 799
BmiI	GGNNCC	6 cut(s)	438, 494, 584, 800, 1306, 1493
BmrFI	CCNGG	3 cut(s)	573, 1098, 1302
BmsI	GCATC	6 cut(s)	246, 382, 388, 834, 1122, 1202
BpiI	GAAGAC	2 cut(s)	128, 1511
BpmI	CTGGAG	2 cut(s)	872, 973
Bpu10I	CCTNAGC	1 cut(s)	482
Bpu14I	TTCGAA	1 cut(s)	268
BpuEI	CTTGAG	1 cut(s)	1204
BpuMI	CCSGG	1 cut(s)	573
BsaAI	YACGTR	1 cut(s)	327
BsaHI	GRCGYC	2 cut(s)	72, 1397
BsaJI	CCNNGG	4 cut(s)	788, 802, 1097, 1300
BsaWI	WCCGGW	1 cut(s)	134
BsaXI	ACNNNNNCTCC	2 cut(s)	723, 753
Bsc4I	CCNNNNNNNGG	5 cut(s)	571, 794, 820, 1240, 1451
Bse1I	ACTGG	3 cut(s)	1183, 1465, 1531
BseBI	CCWGG	2 cut(s)	1098, 1302
BseDI	CCNNGG	4 cut(s)	788, 802, 1097, 1300
BseGI	GGATG	2 cut(s)	849, 1193
BseLI	CCNNNNNNNGG	5 cut(s)	571, 794, 820, 1240, 1451
BseMII	CTCAG	2 cut(s)	496, 624
BseNI	ACTGG	3 cut(s)	1183, 1465, 1531
BseRI	GAGGAG	3 cut(s)	29, 251, 1256
BseXI	GCAGC	5 cut(s)	314, 834, 937, 1303, 1346
BsgI	GTGCAG	2 cut(s)	133, 1353
Bsh1236I	CGCG	1 cut(s)	191
BshFI	GGCC	3 cut(s)	176, 480, 801
BshNI	GGYRCC	2 cut(s)	436, 492
BsiSI	CCGG	2 cut(s)	135, 572
BslFI	GGGAC	2 cut(s)	164, 1012
BslI	CCNNNNNNNGG	5 cut(s)	571, 794, 820, 1240, 1451
BsmAI	GTCTC	1 cut(s)	726
BsmFI	GGGAC	2 cut(s)	164, 1012
BsmI	GAATGC	1 cut(s)	997
BsnI	GGCC	3 cut(s)	176, 480, 801
Bsp119I	TTCGAA	1 cut(s)	268
Bsp143I	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
BspACI	CCGC	7 cut(s)	186, 218, 275, 300, 486, 489, 565
BspANI	GGCC	3 cut(s)	176, 480, 801
BspCNI	CTCAG	2 cut(s)	495, 623
BspFNI	CGCG	1 cut(s)	191
BspLI	GGNNCC	6 cut(s)	438, 494, 584, 800, 1306, 1493
BspMI	ACCTGC	1 cut(s)	1530
BspPI	GGATC	3 cut(s)	82, 655, 1426
BspQI	GCTCTTC	1 cut(s)	946
BspT104I	TTCGAA	1 cut(s)	268
BspT107I	GGYRCC	2 cut(s)	436, 492
BspTI	CTTAAG	2 cut(s)	1108, 1343
BsrI	ACTGG	3 cut(s)	1183, 1465, 1531
BssECI	CCNNGG	4 cut(s)	788, 802, 1097, 1300
BssMI	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
BssNI	GRCGYC	2 cut(s)	72, 1397
BssT1I	CCWWGG	1 cut(s)	802
Bst2UI	CCWGG	2 cut(s)	1098, 1302
Bst4CI	ACNGT	4 cut(s)	289, 948, 1405, 1556
Bst6I	CTCTTC	3 cut(s)	47, 351, 946
BstACI	GRCGYC	2 cut(s)	72, 1397
BstAFI	CTTAAG	2 cut(s)	1108, 1343
BstAPI	GCANNNNNTGC	1 cut(s)	308
BstBAI	YACGTR	1 cut(s)	327
BstBI	TTCGAA	1 cut(s)	268
BstC8I	GCNNGC	2 cut(s)	717, 962
BstDEI	CTNAG	3 cut(s)	482, 610, 1058
BstEII	GGTNACC	1 cut(s)	137
BstENI	CCTNNNNNAGG	1 cut(s)	1238
BstF5I	GGATG	2 cut(s)	849, 1193
BstFNI	CGCG	1 cut(s)	191
BstH2I	RGCGCY	1 cut(s)	610
BstHHI	GCGC	2 cut(s)	191, 609
BstKTI	GATC	5 cut(s)	90, 650, 1286, 1312, 1421
BstMAI	GTCTC	1 cut(s)	726
BstMBI	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
BstMWI	GCNNNNNNNGC	3 cut(s)	308, 456, 486
BstNI	CCWGG	2 cut(s)	1098, 1302
BstPI	GGTNACC	1 cut(s)	137
BstSCI	CCNGG	3 cut(s)	571, 1096, 1300
BstSNI	TACGTA	1 cut(s)	327
BstUI	CGCG	1 cut(s)	191
BstV1I	GCAGC	5 cut(s)	314, 834, 937, 1303, 1346
BstV2I	GAAGAC	2 cut(s)	128, 1511
BstX2I	RGATCY	2 cut(s)	87, 647
BstXI	CCANNNNNNTGG	2 cut(s)	24, 593
BstYI	RGATCY	2 cut(s)	87, 647
BsuI	GTATCC	1 cut(s)	737
BsuRI	GGCC	3 cut(s)	176, 480, 801
BtsCI	GGATG	2 cut(s)	849, 1193
BtsIMutI	CAGTG	3 cut(s)	618, 1524, 1561
BveI	ACCTGC	1 cut(s)	1530
Cac8I	GCNNGC	2 cut(s)	717, 962
CfoI	GCGC	2 cut(s)	191, 609
Cfr13I	GGNCC	3 cut(s)	478, 674, 799
CseI	GACGC	1 cut(s)	61
Csp6I	GTAC	6 cut(s)	316, 328, 361, 437, 493, 1543
CviAII	CATG	4 cut(s)	172, 503, 587, 1496
CviQI	GTAC	6 cut(s)	316, 328, 361, 437, 493, 1543
DdeI	CTNAG	3 cut(s)	482, 610, 1058
DpnI	GATC	5 cut(s)	89, 649, 1285, 1311, 1420
DpnII	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
EaeI	YGGCCR	1 cut(s)	174
Eam1104I	CTCTTC	3 cut(s)	47, 351, 946
EarI	CTCTTC	3 cut(s)	47, 351, 946
Eco105I	TACGTA	1 cut(s)	327
Eco130I	CCWWGG	1 cut(s)	802
Eco47I	GGWCC	1 cut(s)	674
Eco47III	AGCGCT	1 cut(s)	608
Eco57I	CTGAAG	1 cut(s)	375
Eco91I	GGTNACC	1 cut(s)	137
EcoNI	CCTNNNNNAGG	1 cut(s)	1238
EcoO109I	RGGNCCY	1 cut(s)	674
EcoO65I	GGTNACC	1 cut(s)	137
EcoRI	GAATTC	1 cut(s)	241
EcoRII	CCWGG	2 cut(s)	1096, 1300
EcoT14I	CCWWGG	1 cut(s)	802
ErhI	CCWWGG	1 cut(s)	802
FaeI	CATG	4 cut(s)	175, 506, 590, 1499
FaqI	GGGAC	2 cut(s)	164, 1012
FatI	CATG	4 cut(s)	171, 502, 586, 1495
FauI	CCCGC	1 cut(s)	482
FblI	GTMKAC	2 cut(s)	1083, 1400
Fnu4HI	GCNGC	7 cut(s)	187, 303, 487, 848, 926, 1317, 1335
FokI	GGATG	2 cut(s)	856, 1180
Fsp4HI	GCNGC	7 cut(s)	187, 303, 487, 848, 926, 1317, 1335
FspBI	CTAG	2 cut(s)	183, 1597
GlaI	GCGC	2 cut(s)	190, 608
GluI	GCNGC	7 cut(s)	187, 303, 487, 848, 926, 1317, 1335
GsuI	CTGGAG	2 cut(s)	872, 973
HaeII	RGCGCY	1 cut(s)	610
HaeIII	GGCC	3 cut(s)	176, 480, 801
HapII	CCGG	2 cut(s)	135, 572
HgaI	GACGC	1 cut(s)	61
HhaI	GCGC	2 cut(s)	191, 609
Hin1I	GRCGYC	2 cut(s)	72, 1397
Hin1II	CATG	4 cut(s)	175, 506, 590, 1499
Hin6I	GCGC	2 cut(s)	189, 607
HinP1I	GCGC	2 cut(s)	189, 607
HincII	GTYRAC	3 cut(s)	809, 904, 1278
HindII	GTYRAC	3 cut(s)	809, 904, 1278
HindIII	AAGCTT	1 cut(s)	1483
HinfI	GANTC	7 cut(s)	81, 131, 1000, 1020, 1093, 1349, 1472
HpaI	GTTAAC	1 cut(s)	1278
HpaII	CCGG	2 cut(s)	135, 572
HphI	GGTGA	7 cut(s)	25, 149, 212, 587, 936, 1193, 1222
Hpy166II	GTNNAC	5 cut(s)	809, 904, 1084, 1278, 1401
Hpy188I	TCNGA	3 cut(s)	130, 661, 789
Hpy188III	TCNNGA	7 cut(s)	85, 283, 335, 412, 677, 952, 1048
Hpy8I	GTNNAC	5 cut(s)	809, 904, 1084, 1278, 1401
Hpy99I	CGWCG	3 cut(s)	71, 77, 1399
HpyAV	CCTTC	3 cut(s)	506, 768, 819
HpyCH4III	ACNGT	4 cut(s)	289, 948, 1405, 1556
HpyCH4IV	ACGT	4 cut(s)	326, 1071, 1208, 1397
HpyF10VI	GCNNNNNNNGC	3 cut(s)	308, 456, 486
HpyF3I	CTNAG	3 cut(s)	482, 610, 1058
HpySE526I	ACGT	4 cut(s)	326, 1071, 1208, 1397
Hsp92I	GRCGYC	2 cut(s)	72, 1397
Hsp92II	CATG	4 cut(s)	175, 506, 590, 1499
HspAI	GCGC	2 cut(s)	189, 607
KpnI	GGTACC	2 cut(s)	440, 496
KspAI	GTTAAC	1 cut(s)	1278
Kzo9I	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
LguI	GCTCTTC	1 cut(s)	946
LmnI	GCTCC	1 cut(s)	1243
Lsp1109I	GCAGC	5 cut(s)	314, 834, 937, 1303, 1346
LweI	GCATC	6 cut(s)	246, 382, 388, 834, 1122, 1202
MaeI	CTAG	2 cut(s)	183, 1597
MaeII	ACGT	4 cut(s)	326, 1071, 1208, 1397
MaeIII	GTNAC	4 cut(s)	137, 419, 1021, 1228
MalI	GATC	5 cut(s)	89, 649, 1285, 1311, 1420
MboI	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
MboII	GAAGA	9 cut(s)	34, 49, 133, 169, 265, 368, 933, 1459, 1516
MflI	RGATCY	2 cut(s)	87, 647
MlyI	GAGTC	6 cut(s)	90, 994, 1029, 1102, 1343, 1466
MmeI	TCCRAC	2 cut(s)	58, 721
MslI	CAYNNNNRTG	1 cut(s)	1235
MspA1I	CMGCKG	1 cut(s)	486
MspCI	CTTAAG	2 cut(s)	1108, 1343
MspI	CCGG	2 cut(s)	135, 572
MspR9I	CCNGG	3 cut(s)	573, 1098, 1302
Mva1269I	GAATGC	1 cut(s)	997
MvaI	CCWGG	2 cut(s)	1098, 1302
MvnI	CGCG	1 cut(s)	191
MwoI	GCNNNNNNNGC	3 cut(s)	308, 456, 486
NciI	CCSGG	1 cut(s)	573
NdeII	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
NlaIII	CATG	4 cut(s)	175, 506, 590, 1499
NlaIV	GGNNCC	6 cut(s)	438, 494, 584, 800, 1306, 1493
NmeAIII	GCCGAG	1 cut(s)	1402
NmuCI	GTSAC	3 cut(s)	137, 1021, 1228
NspV	TTCGAA	1 cut(s)	268
PciSI	GCTCTTC	1 cut(s)	946
PcsI	WCGNNNNNNNCGW	1 cut(s)	114
PctI	GAATGC	1 cut(s)	997
PfeI	GAWTC	1 cut(s)	131
PflFI	GACNNNGTC	1 cut(s)	71
PflMI	CCANNNNNTGG	1 cut(s)	820
PkrI	GCNGC	7 cut(s)	188, 304, 488, 849, 927, 1318, 1336
PleI	GAGTC	6 cut(s)	89, 994, 1028, 1101, 1343, 1466
PpsI	GAGTC	6 cut(s)	89, 994, 1028, 1101, 1343, 1466
Ppu21I	YACGTR	1 cut(s)	327
PpuMI	RGGWCCY	1 cut(s)	674
PshBI	ATTAAT	1 cut(s)	1035
PsiI	TTATAA	1 cut(s)	1359
Psp1406I	AACGTT	1 cut(s)	1208
Psp5II	RGGWCCY	1 cut(s)	674
Psp6I	CCWGG	2 cut(s)	1096, 1300
PspEI	GGTNACC	1 cut(s)	137
PspGI	CCWGG	2 cut(s)	1096, 1300
PspN4I	GGNNCC	6 cut(s)	438, 494, 584, 800, 1306, 1493
PspPI	GGNCC	3 cut(s)	478, 674, 799
PspPPI	RGGWCCY	1 cut(s)	674
PsuI	RGATCY	2 cut(s)	87, 647
PsyI	GACNNNGTC	1 cut(s)	71
RsaI	GTAC	6 cut(s)	317, 329, 362, 438, 494, 1544
RsaNI	GTAC	6 cut(s)	316, 328, 361, 437, 493, 1543
RseI	CAYNNNNRTG	1 cut(s)	1235
SapI	GCTCTTC	1 cut(s)	946
SatI	GCNGC	7 cut(s)	187, 303, 487, 848, 926, 1317, 1335
Sau3AI	GATC	5 cut(s)	87, 647, 1283, 1309, 1418
Sau96I	GGNCC	3 cut(s)	478, 674, 799
ScaI	AGTACT	1 cut(s)	362
SchI	GAGTC	6 cut(s)	90, 994, 1029, 1102, 1343, 1466
ScrFI	CCNGG	3 cut(s)	573, 1098, 1302
SfaNI	GCATC	6 cut(s)	246, 382, 388, 834, 1122, 1202
SfuI	TTCGAA	1 cut(s)	268
SinI	GGWCC	1 cut(s)	674
SmiMI	CAYNNNNRTG	1 cut(s)	1235
SmlI	CTYRAG	3 cut(s)	1108, 1219, 1343
SmoI	CTYRAG	3 cut(s)	1108, 1219, 1343
SnaBI	TACGTA	1 cut(s)	327
SsiI	CCGC	7 cut(s)	186, 218, 275, 300, 486, 489, 565
SspI	AATATT	1 cut(s)	754
SspMI	CTAG	2 cut(s)	183, 1597
StyD4I	CCNGG	3 cut(s)	571, 1096, 1300
StyI	CCWWGG	1 cut(s)	802
TaaI	ACNGT	4 cut(s)	289, 948, 1405, 1556
TaiI	ACGT	4 cut(s)	329, 1074, 1211, 1400
TaqI	TCGA	9 cut(s)	101, 108, 209, 228, 268, 334, 765, 1282, 1470
TatI	WGTACW	1 cut(s)	360
TauI	GCSGC	2 cut(s)	189, 489
TfiI	GAWTC	1 cut(s)	131
TscAI	CASTG	3 cut(s)	618, 1531, 1561
TseFI	GTSAC	3 cut(s)	137, 1021, 1228
TseI	GCWGC	5 cut(s)	302, 847, 925, 1316, 1334
Tsp45I	GTSAC	3 cut(s)	137, 1021, 1228
TspDTI	ATGAA	8 cut(s)	49, 443, 488, 548, 750, 1243, 1274, 1510
TspRI	CASTG	3 cut(s)	618, 1531, 1561
Tth111I	GACNNNGTC	1 cut(s)	71
Van91I	CCANNNNNTGG	1 cut(s)	820
Vha464I	CTTAAG	2 cut(s)	1108, 1343
VpaK11BI	GGWCC	1 cut(s)	674
VspI	ATTAAT	1 cut(s)	1035
XagI	CCTNNNNNAGG	1 cut(s)	1238
XapI	RAATTY	3 cut(s)	241, 817, 979
XcmI	CCANNNNNNNNNTGG	2 cut(s)	21, 1094
XmiI	GTMKAC	2 cut(s)	1083, 1400
XspI	CTAG	2 cut(s)	183, 1597
ZraI	GACGTC	1 cut(s)	1398
ZrmI	AGTACT	1 cut(s)	362

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.