Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

16424489 .. 16430684

Gene Structure 6196 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG178600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1662 bp

ATGAGAATCCCTCCACCAAAATTATGGCTCACCATCCTCCTCTTCCTCCCATGCTTCATCAGCGACGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTCCGACTCCGGTGACTTGAATTGCACTAAGAAGATCGTCCTCAACATGGCTGTCCCCAGTGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAGATTGTCGAGGTAGAGGAGAATTCAAGCAACAAGATGCAAACTTTTCGAATACCGCCTGTCCTGACTGTCAATAAATCCGCGGCTTATGCCTTGTACGAGCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACTCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTGAAGATATGTGAGAGGTTGCGAGATGAAAATGGTACCATTCTTGAGCATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCATGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCCAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGGATCTAAAGTTTCGAAAGTGCTTGTAGGTCCTGAAAACCGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGACTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGTGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAGTTATGGAATTTCTTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGGAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAGAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGCTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAACGTTATCTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGGGCCGAGTGCCAATTTTCCACGACATCTACTGTCATTGATAATGGATCACAGGGTAATCCCTTTAAGCCACCAAAGGCAGGAACAACTGGTTTCTTTGACTCCATTGAAAGCTTTTGGAACCATGTGTGGGAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGTCATTGTGCTACTGTGGCTTTTACATCAGAAGGGACTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

553

Amino Acids

61.68

Weight (kDa)

5.89

Isoelectric Point (pI)

34.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	46 - 543	5.2e-152	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1437
AatII	GACGTC	1 cut(s)	69
Acc65I	GGTACC	2 cut(s)	437, 493
AccB1I	GGYRCC	3 cut(s)	437, 493, 1018
AccB7I	CCANNNNNTGG	2 cut(s)	817, 1028
AccI	GTMKAC	1 cut(s)	1161
AccII	CGCG	2 cut(s)	185, 296
AciI	CCGC	7 cut(s)	212, 269, 294, 296, 487, 490, 562
AclI	AACGTT	2 cut(s)	1286, 1352
AclWI	GGATC	3 cut(s)	76, 652, 1504
AcsI	RAATTY	3 cut(s)	235, 814, 976
AcuI	CTGAAG	1 cut(s)	369
AcyI	GRCGYC	1 cut(s)	66
AfaI	GTAC	5 cut(s)	311, 323, 356, 439, 495
AfeI	AGCGCT	1 cut(s)	605
AfiI	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
AflII	CTTAAG	2 cut(s)	1186, 1421
AgsI	TTSAA	6 cut(s)	139, 240, 632, 881, 1369, 1559
AjnI	CCWGG	3 cut(s)	799, 1174, 1378
AloI	GAACNNNNNNTCC	4 cut(s)	606, 638, 1028, 1060
AluBI	AGCT	5 cut(s)	316, 716, 1394, 1415, 1563
AluI	AGCT	5 cut(s)	316, 716, 1394, 1415, 1563
Alw26I	GTCTC	2 cut(s)	723, 1577
AlwI	GGATC	3 cut(s)	76, 652, 1504
Aor51HI	AGCGCT	1 cut(s)	605
AoxI	GGCC	4 cut(s)	479, 796, 1212, 1452
ApeKI	GCWGC	4 cut(s)	844, 922, 1394, 1412
ApoI	RAATTY	3 cut(s)	235, 814, 976
AseI	ATTAAT	1 cut(s)	1113
Asp718I	GGTACC	2 cut(s)	437, 493
AspLEI	GCGC	2 cut(s)	185, 606
AspS9I	GGNCC	5 cut(s)	479, 671, 797, 1213, 1452
AsuC2I	CCSGG	1 cut(s)	570
AsuHPI	GGTGA	7 cut(s)	22, 143, 206, 584, 933, 1271, 1300
AsuII	TTCGAA	3 cut(s)	262, 656, 1047
AvaII	GGWCC	1 cut(s)	671
BanI	GGYRCC	3 cut(s)	437, 493, 1018
BbsI	GAAGAC	1 cut(s)	122
BbvCI	CCTCAGC	1 cut(s)	483
BbvI	GCAGC	4 cut(s)	831, 934, 1381, 1424
BccI	CCATC	2 cut(s)	41, 866
BcgI	CGANNNNNNTGC	4 cut(s)	242, 276, 1376, 1410
BciT130I	CCWGG	3 cut(s)	801, 1176, 1380
BciVI	GTATCC	1 cut(s)	734
BcnI	CCSGG	1 cut(s)	570
BcoDI	GTCTC	2 cut(s)	723, 1577
BfoI	RGCGCY	1 cut(s)	607
BfrI	CTTAAG	2 cut(s)	1186, 1421
BfuI	GTATCC	1 cut(s)	734
BglI	GCCNNNNNGGC	1 cut(s)	487
BisI	GCNGC	6 cut(s)	297, 488, 845, 923, 1395, 1413
BlsI	GCNGC	6 cut(s)	298, 489, 846, 924, 1396, 1414
BmcAI	AGTACT	1 cut(s)	356
Bme1390I	CCNGG	4 cut(s)	570, 801, 1176, 1380
Bme18I	GGWCC	1 cut(s)	671
BmgT120I	GGNCC	5 cut(s)	479, 671, 797, 1213, 1452
BmiI	GGNNCC	6 cut(s)	439, 495, 581, 1020, 1384, 1571
BmrFI	CCNGG	4 cut(s)	570, 801, 1176, 1380
BmrI	ACTGGG	1 cut(s)	171
BmsI	GCATC	7 cut(s)	240, 376, 382, 831, 1072, 1200, 1280
BmuI	ACTGGG	1 cut(s)	171
BpiI	GAAGAC	1 cut(s)	122
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	2 cut(s)	869, 970
Bpu10I	CCTNAGC	1 cut(s)	483
Bpu14I	TTCGAA	3 cut(s)	262, 656, 1047
BpuEI	CTTGAG	2 cut(s)	467, 1282
BpuMI	CCSGG	1 cut(s)	570
BsaAI	YACGTR	1 cut(s)	321
BsaHI	GRCGYC	1 cut(s)	66
BsaJI	CCNNGG	5 cut(s)	294, 785, 799, 1175, 1378
BsaWI	WCCGGW	1 cut(s)	128
BsaXI	ACNNNNNCTCC	2 cut(s)	720, 750
Bsc4I	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
Bse1I	ACTGG	4 cut(s)	177, 1261, 1543, 1609
BseBI	CCWGG	3 cut(s)	801, 1176, 1380
BseDI	CCNNGG	5 cut(s)	294, 785, 799, 1175, 1378
BseGI	GGATG	4 cut(s)	33, 846, 1063, 1271
BseLI	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
BseMII	CTCAG	2 cut(s)	497, 621
BseNI	ACTGG	4 cut(s)	177, 1261, 1543, 1609
BseRI	GAGGAG	3 cut(s)	29, 245, 1334
BseXI	GCAGC	4 cut(s)	831, 934, 1381, 1424
BsgI	GTGCAG	1 cut(s)	1431
Bsh1236I	CGCG	2 cut(s)	185, 296
BshFI	GGCC	4 cut(s)	481, 798, 1214, 1454
BshNI	GGYRCC	3 cut(s)	437, 493, 1018
BsiSI	CCGG	2 cut(s)	129, 569
BslFI	GGGAC	2 cut(s)	158, 1090
BslI	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
BsmAI	GTCTC	2 cut(s)	723, 1577
BsmFI	GGGAC	2 cut(s)	158, 1090
BsnI	GGCC	4 cut(s)	481, 798, 1214, 1454
Bsp119I	TTCGAA	3 cut(s)	262, 656, 1047
Bsp143I	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
BspACI	CCGC	7 cut(s)	212, 269, 294, 296, 487, 490, 562
BspANI	GGCC	4 cut(s)	481, 798, 1214, 1454
BspCNI	CTCAG	2 cut(s)	496, 620
BspFNI	CGCG	2 cut(s)	185, 296
BspLI	GGNNCC	6 cut(s)	439, 495, 581, 1020, 1384, 1571
BspPI	GGATC	3 cut(s)	76, 652, 1504
BspQI	GCTCTTC	1 cut(s)	943
BspT104I	TTCGAA	3 cut(s)	262, 656, 1047
BspT107I	GGYRCC	3 cut(s)	437, 493, 1018
BspTI	CTTAAG	2 cut(s)	1186, 1421
BsrI	ACTGG	4 cut(s)	177, 1261, 1543, 1609
BssECI	CCNNGG	5 cut(s)	294, 785, 799, 1175, 1378
BssMI	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
BssNI	GRCGYC	1 cut(s)	66
Bst2UI	CCWGG	3 cut(s)	801, 1176, 1380
Bst4CI	ACNGT	4 cut(s)	283, 1001, 1483, 1633
Bst6I	CTCTTC	3 cut(s)	47, 345, 943
BstACI	GRCGYC	1 cut(s)	66
BstAFI	CTTAAG	2 cut(s)	1186, 1421
BstBAI	YACGTR	1 cut(s)	321
BstBI	TTCGAA	3 cut(s)	262, 656, 1047
BstC8I	GCNNGC	2 cut(s)	714, 959
BstDEI	CTNAG	4 cut(s)	147, 483, 607, 1136
BstDSI	CCRYGG	1 cut(s)	294
BstENI	CCTNNNNNAGG	1 cut(s)	1316
BstF5I	GGATG	4 cut(s)	33, 846, 1063, 1271
BstFNI	CGCG	2 cut(s)	185, 296
BstH2I	RGCGCY	1 cut(s)	607
BstHHI	GCGC	2 cut(s)	185, 606
BstKTI	GATC	6 cut(s)	84, 156, 647, 1364, 1390, 1499
BstMAI	GTCTC	2 cut(s)	723, 1577
BstMBI	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
BstMWI	GCNNNNNNNGC	5 cut(s)	60, 302, 457, 487, 1634
BstNI	CCWGG	3 cut(s)	801, 1176, 1380
BstSCI	CCNGG	4 cut(s)	568, 799, 1174, 1378
BstSNI	TACGTA	1 cut(s)	321
BstUI	CGCG	2 cut(s)	185, 296
BstV1I	GCAGC	4 cut(s)	831, 934, 1381, 1424
BstV2I	GAAGAC	1 cut(s)	122
BstX2I	RGATCY	2 cut(s)	81, 644
BstXI	CCANNNNNNTGG	2 cut(s)	24, 590
BstYI	RGATCY	2 cut(s)	81, 644
BsuI	GTATCC	1 cut(s)	734
BsuRI	GGCC	4 cut(s)	481, 798, 1214, 1454
BtgI	CCRYGG	1 cut(s)	294
BtsCI	GGATG	4 cut(s)	33, 846, 1063, 1271
BtsIMutI	CAGTG	3 cut(s)	184, 615, 1602
Cac8I	GCNNGC	2 cut(s)	714, 959
CfoI	GCGC	2 cut(s)	185, 606
Cfr13I	GGNCC	5 cut(s)	479, 671, 797, 1213, 1452
Cfr42I	CCGCGG	1 cut(s)	297
Csp6I	GTAC	5 cut(s)	310, 322, 355, 438, 494
CviAII	CATG	5 cut(s)	51, 166, 504, 584, 1574
CviQI	GTAC	5 cut(s)	310, 322, 355, 438, 494
DdeI	CTNAG	4 cut(s)	147, 483, 607, 1136
DpnI	GATC	6 cut(s)	83, 155, 646, 1363, 1389, 1498
DpnII	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
Eam1104I	CTCTTC	3 cut(s)	47, 345, 943
EarI	CTCTTC	3 cut(s)	47, 345, 943
Eco105I	TACGTA	1 cut(s)	321
Eco47I	GGWCC	1 cut(s)	671
Eco47III	AGCGCT	1 cut(s)	605
Eco57I	CTGAAG	1 cut(s)	369
EcoNI	CCTNNNNNAGG	1 cut(s)	1316
EcoO109I	RGGNCCY	2 cut(s)	671, 1213
EcoRI	GAATTC	1 cut(s)	235
EcoRII	CCWGG	3 cut(s)	799, 1174, 1378
FaeI	CATG	5 cut(s)	54, 169, 507, 587, 1577
FaqI	GGGAC	2 cut(s)	158, 1090
FatI	CATG	5 cut(s)	50, 165, 503, 583, 1573
FauI	CCCGC	1 cut(s)	483
FblI	GTMKAC	1 cut(s)	1161
Fnu4HI	GCNGC	6 cut(s)	297, 488, 845, 923, 1395, 1413
FokI	GGATG	4 cut(s)	20, 853, 1050, 1258
Fsp4HI	GCNGC	6 cut(s)	297, 488, 845, 923, 1395, 1413
GlaI	GCGC	2 cut(s)	184, 605
GluI	GCNGC	6 cut(s)	297, 488, 845, 923, 1395, 1413
GsuI	CTGGAG	2 cut(s)	869, 970
HaeII	RGCGCY	1 cut(s)	607
HaeIII	GGCC	4 cut(s)	481, 798, 1214, 1454
HapII	CCGG	2 cut(s)	129, 569
HhaI	GCGC	2 cut(s)	185, 606
Hin1I	GRCGYC	1 cut(s)	66
Hin1II	CATG	5 cut(s)	54, 169, 507, 587, 1577
Hin6I	GCGC	2 cut(s)	183, 604
HinP1I	GCGC	2 cut(s)	183, 604
HincII	GTYRAC	2 cut(s)	806, 901
HindII	GTYRAC	2 cut(s)	806, 901
HindIII	AAGCTT	1 cut(s)	1561
HinfI	GANTC	9 cut(s)	6, 75, 125, 367, 1078, 1098, 1171, 1427, 1550
HpaII	CCGG	2 cut(s)	129, 569
HphI	GGTGA	7 cut(s)	22, 143, 206, 584, 933, 1271, 1300
Hpy166II	GTNNAC	3 cut(s)	806, 901, 1162
Hpy188I	TCNGA	3 cut(s)	124, 786, 1648
Hpy188III	TCNNGA	8 cut(s)	79, 277, 329, 446, 674, 949, 1126, 1359
Hpy8I	GTNNAC	3 cut(s)	806, 901, 1162
Hpy99I	CGWCG	2 cut(s)	68, 71
HpyAV	CCTTC	5 cut(s)	507, 765, 816, 1031, 1643
HpyCH4III	ACNGT	4 cut(s)	283, 1001, 1483, 1633
HpyCH4IV	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
HpyF10VI	GCNNNNNNNGC	5 cut(s)	60, 302, 457, 487, 1634
HpyF3I	CTNAG	4 cut(s)	147, 483, 607, 1136
HpySE526I	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
Hsp92I	GRCGYC	1 cut(s)	66
Hsp92II	CATG	5 cut(s)	54, 169, 507, 587, 1577
HspAI	GCGC	2 cut(s)	183, 604
KpnI	GGTACC	2 cut(s)	441, 497
KspI	CCGCGG	1 cut(s)	297
Kzo9I	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
LguI	GCTCTTC	1 cut(s)	943
LmnI	GCTCC	1 cut(s)	1321
Lsp1109I	GCAGC	4 cut(s)	831, 934, 1381, 1424
LweI	GCATC	7 cut(s)	240, 376, 382, 831, 1072, 1200, 1280
MaeII	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
MaeIII	GTNAC	3 cut(s)	131, 1099, 1306
MalI	GATC	6 cut(s)	83, 155, 646, 1363, 1389, 1498
MboI	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
MboII	GAAGA	6 cut(s)	34, 127, 163, 362, 418, 930
MflI	RGATCY	2 cut(s)	81, 644
MlyI	GAGTC	8 cut(s)	84, 119, 361, 1072, 1107, 1180, 1421, 1544
MmeI	TCCRAC	3 cut(s)	52, 147, 718
MseI	TTAA	7 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515
MslI	CAYNNNNRTG	2 cut(s)	1068, 1313
MspA1I	CMGCKG	2 cut(s)	296, 487
MspCI	CTTAAG	2 cut(s)	1186, 1421
MspI	CCGG	2 cut(s)	129, 569
MspR9I	CCNGG	4 cut(s)	570, 801, 1176, 1380
MvaI	CCWGG	3 cut(s)	801, 1176, 1380
MvnI	CGCG	2 cut(s)	185, 296
MwoI	GCNNNNNNNGC	5 cut(s)	60, 302, 457, 487, 1634
NciI	CCSGG	1 cut(s)	570
NdeII	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
NlaIII	CATG	5 cut(s)	54, 169, 507, 587, 1577
NlaIV	GGNNCC	6 cut(s)	439, 495, 581, 1020, 1384, 1571
NmeAIII	GCCGAG	1 cut(s)	1480
NmuCI	GTSAC	3 cut(s)	131, 1099, 1306
NspV	TTCGAA	3 cut(s)	262, 656, 1047
PciSI	GCTCTTC	1 cut(s)	943
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PfeI	GAWTC	1 cut(s)	6
PflMI	CCANNNNNTGG	2 cut(s)	817, 1028
PkrI	GCNGC	6 cut(s)	298, 489, 846, 924, 1396, 1414
PleI	GAGTC	8 cut(s)	83, 119, 361, 1072, 1106, 1179, 1421, 1544
PpsI	GAGTC	8 cut(s)	83, 119, 361, 1072, 1106, 1179, 1421, 1544
Ppu21I	YACGTR	1 cut(s)	321
PpuMI	RGGWCCY	1 cut(s)	671
PshBI	ATTAAT	1 cut(s)	1113
PsiI	TTATAA	1 cut(s)	1437
Psp1406I	AACGTT	2 cut(s)	1286, 1352
Psp5II	RGGWCCY	1 cut(s)	671
Psp6I	CCWGG	3 cut(s)	799, 1174, 1378
PspGI	CCWGG	3 cut(s)	799, 1174, 1378
PspN4I	GGNNCC	6 cut(s)	439, 495, 581, 1020, 1384, 1571
PspPI	GGNCC	5 cut(s)	479, 671, 797, 1213, 1452
PspPPI	RGGWCCY	1 cut(s)	671
PsuI	RGATCY	2 cut(s)	81, 644
RsaI	GTAC	5 cut(s)	311, 323, 356, 439, 495
RsaNI	GTAC	5 cut(s)	310, 322, 355, 438, 494
RseI	CAYNNNNRTG	2 cut(s)	1068, 1313
SacII	CCGCGG	1 cut(s)	297
SapI	GCTCTTC	1 cut(s)	943
SaqAI	TTAA	7 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515
SatI	GCNGC	6 cut(s)	297, 488, 845, 923, 1395, 1413
Sau3AI	GATC	6 cut(s)	81, 153, 644, 1361, 1387, 1496
Sau96I	GGNCC	5 cut(s)	479, 671, 797, 1213, 1452
ScaI	AGTACT	1 cut(s)	356
SchI	GAGTC	8 cut(s)	84, 119, 361, 1072, 1107, 1180, 1421, 1544
ScrFI	CCNGG	4 cut(s)	570, 801, 1176, 1380
SfaNI	GCATC	7 cut(s)	240, 376, 382, 831, 1072, 1200, 1280
Sfr303I	CCGCGG	1 cut(s)	297
SfuI	TTCGAA	3 cut(s)	262, 656, 1047
SgrBI	CCGCGG	1 cut(s)	297
SinI	GGWCC	1 cut(s)	671
SmiMI	CAYNNNNRTG	2 cut(s)	1068, 1313
SmlI	CTYRAG	4 cut(s)	446, 1186, 1297, 1421
SmoI	CTYRAG	4 cut(s)	446, 1186, 1297, 1421
SnaBI	TACGTA	1 cut(s)	321
SsiI	CCGC	7 cut(s)	212, 269, 294, 296, 487, 490, 562
SspI	AATATT	1 cut(s)	751
StyD4I	CCNGG	4 cut(s)	568, 799, 1174, 1378
TaaI	ACNGT	4 cut(s)	283, 1001, 1483, 1633
TaiI	ACGT	5 cut(s)	69, 323, 1152, 1289, 1355
TatI	WGTACW	1 cut(s)	354
TauI	GCSGC	2 cut(s)	299, 490
TfiI	GAWTC	1 cut(s)	6
Tru1I	TTAA	7 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515
Tru9I	TTAA	7 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515
TscAI	CASTG	3 cut(s)	184, 615, 1609
TseFI	GTSAC	3 cut(s)	131, 1099, 1306
TseI	GCWGC	4 cut(s)	844, 922, 1394, 1412
Tsp45I	GTSAC	3 cut(s)	131, 1099, 1306
TspDTI	ATGAA	9 cut(s)	46, 444, 489, 545, 747, 1321, 1352, 1455, 1588
TspRI	CASTG	3 cut(s)	184, 615, 1609
Van91I	CCANNNNNTGG	2 cut(s)	817, 1028
Vha464I	CTTAAG	2 cut(s)	1186, 1421
VpaK11BI	GGWCC	1 cut(s)	671
VspI	ATTAAT	1 cut(s)	1113
XagI	CCTNNNNNAGG	1 cut(s)	1316
XapI	RAATTY	3 cut(s)	235, 814, 976
XcmI	CCANNNNNNNNNTGG	2 cut(s)	21, 1172
XmiI	GTMKAC	1 cut(s)	1161
ZraI	GACGTC	1 cut(s)	67
ZrmI	AGTACT	1 cut(s)	356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.