Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

38173262 .. 38180209

Gene Structure 6948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG175700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGATGAGGTTCTTCAAGCTTGTTCAGCAGCACTTAGCGTCAGGCTCTGATGGTGAAGATAATGGAAGTTCAAAGATTTCAGAGGAGGATTTGGAGACCCCATTTGTTGACTTCTTACAGACACGGCTGCCTCCCAAGATAAAATCGTACAGATCATATCGCCTTTCTCTATCTACATTCCCAAAAATGGGAAACCCTCCACCAAAATTATGGCTTATCACCCTCCTCTTCCTCCCTTGCTTCTTCATAAGCGACGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTCCGAATCCGATGAACTTAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGCGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAAATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCACCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACGAGCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTCAAGATATGTGAGAGGTTACGAGATGAAAATGGTACCATTATTGAACATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCTTGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCGAATACAGCCCATTGTGTCCGCTTTCTGGGTGATTGGTTCCATGTTTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTTATTCAAGTAAAGACAGGATCTAAAGTTTCGGAAGTGCTTGTAGGTCCTGAAAACAGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTAATTGGAGACTTTGTTGGACACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCAAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTCAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAGTTATGGAATTTCTTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTCGAAGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAGAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGTTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAATTTTAACTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGAGCCGAGTGCCAATTTTCTACAACGTCTACTGTCATTGATAATGGATCACAGGTTAATCCCTTTAAGCCACCAAAGACAGGAACAACTGGTTTCTTTGACTCCATTGAAAGCTTTTGGAACCATGCGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTTGGTCTACTTTTGGCAATTTTTCCAACAGTCATTGTGCTACTGTGGCTTTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAGGATAGTCCGAGCACCATGAATACTAGACACAGAATTAATGTAGATCATCGACATGTTCATGTCCATAAACATCATGGGCATGAAACAAGGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGATTACCATTGCGACCTTCACCATGTCCATAAGGATAAGCACAAGCATGGCCGAAGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCATGGCCAGAAAAAAACAAAGGAGAACAACAAAAGACTATTCAAAGTGACCAGAGTCACCAGACACACAATCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

778

Amino Acids

89.0

Weight (kDa)

8.53

Isoelectric Point (pI)

40.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	109 - 621	1.4e-159	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1626
AatII	GACGTC	1 cut(s)	258
Acc65I	GGTACC	2 cut(s)	626, 682
AccB1I	GGYRCC	4 cut(s)	626, 682, 1207, 2079
AccB7I	CCANNNNNTGG	2 cut(s)	1006, 1217
AccI	GTMKAC	3 cut(s)	1350, 1667, 1885
AccII	CGCG	1 cut(s)	374
AciI	CCGC	6 cut(s)	369, 401, 483, 676, 679, 751
AclI	AACGTT	1 cut(s)	1475
AclWI	GGATC	4 cut(s)	265, 841, 1693, 1946
AcoI	YGGCCR	3 cut(s)	357, 2206, 2263
AcsI	RAATTY	4 cut(s)	424, 1003, 1165, 1539
AcuI	CTGAAG	2 cut(s)	558, 1819
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	7 cut(s)	149, 500, 512, 545, 628, 684, 1853
AfeI	AGCGCT	1 cut(s)	794
AflII	CTTAAG	2 cut(s)	1375, 1610
AflIII	ACRYGT	1 cut(s)	2041
AjnI	CCWGG	3 cut(s)	988, 1363, 1567
AloI	GAACNNNNNNTCC	4 cut(s)	795, 827, 1217, 1249
AluBI	AGCT	7 cut(s)	19, 488, 505, 905, 1583, 1604, 1752
AluI	AGCT	7 cut(s)	19, 488, 505, 905, 1583, 1604, 1752
Alw21I	GWGCWC	1 cut(s)	2003
Alw26I	GTCTC	2 cut(s)	89, 912
AlwI	GGATC	4 cut(s)	265, 841, 1693, 1946
AlwNI	CAGNNNCTG	1 cut(s)	47
Aor51HI	AGCGCT	1 cut(s)	794
AoxI	GGCC	5 cut(s)	357, 668, 985, 2206, 2263
ApaI	GGGCCC	1 cut(s)	989
ApeKI	GCWGC	7 cut(s)	28, 127, 485, 1033, 1111, 1583, 1601
ApoI	RAATTY	4 cut(s)	424, 1003, 1165, 1539
AseI	ATTAAT	2 cut(s)	1302, 2025
Asp700I	GAANNNNTTC	1 cut(s)	2218
Asp718I	GGTACC	2 cut(s)	626, 682
AspLEI	GCGC	2 cut(s)	374, 795
AspS9I	GGNCC	4 cut(s)	668, 860, 985, 986
AsuHPI	GGTGA	9 cut(s)	65, 211, 395, 773, 1122, 1460, 1489, 2168, 2308
AsuII	TTCGAA	3 cut(s)	451, 1236, 1398
AvaII	GGWCC	1 cut(s)	860
BaeGI	GKGCMC	1 cut(s)	989
BalI	TGGCCA	1 cut(s)	2265
BanI	GGYRCC	4 cut(s)	626, 682, 1207, 2079
BanII	GRGCYC	1 cut(s)	989
BbsI	GAAGAC	2 cut(s)	311, 1778
Bbv12I	GWGCWC	1 cut(s)	2003
BbvCI	CCTCAGC	1 cut(s)	672
BbvI	GCAGC	7 cut(s)	40, 114, 497, 1020, 1123, 1570, 1613
BccI	CCATC	2 cut(s)	44, 1055
BceAI	ACGGC	2 cut(s)	140, 344
BcgI	CGANNNNNNTGC	4 cut(s)	431, 465, 1565, 1599
BciT130I	CCWGG	3 cut(s)	990, 1365, 1569
BciVI	GTATCC	1 cut(s)	2249
BclI	TGATCA	2 cut(s)	1975, 2125
BcoDI	GTCTC	2 cut(s)	89, 912
BfaI	CTAG	2 cut(s)	366, 2013
BfoI	RGCGCY	1 cut(s)	796
BfrI	CTTAAG	2 cut(s)	1375, 1610
BfuI	GTATCC	1 cut(s)	2249
BglI	GCCNNNNNGGC	1 cut(s)	676
BisI	GCNGC	9 cut(s)	29, 128, 370, 486, 677, 1034, 1112, 1584, 1602
BlsI	GCNGC	9 cut(s)	30, 129, 371, 487, 678, 1035, 1113, 1585, 1603
BmcAI	AGTACT	1 cut(s)	545
Bme1390I	CCNGG	3 cut(s)	990, 1365, 1569
Bme18I	GGWCC	1 cut(s)	860
BmgT120I	GGNCC	4 cut(s)	668, 860, 985, 986
BmiI	GGNNCC	9 cut(s)	628, 684, 770, 986, 987, 1209, 1573, 1760, 2081
BmrFI	CCNGG	3 cut(s)	990, 1365, 1569
BmsI	GCATC	8 cut(s)	429, 565, 571, 1020, 1261, 1389, 1469, 1829
BoxI	GACNNNNGTC	1 cut(s)	2312
BpiI	GAAGAC	2 cut(s)	311, 1778
BpmI	CTGGAG	2 cut(s)	1058, 1159
Bpu10I	CCTNAGC	1 cut(s)	672
Bpu14I	TTCGAA	3 cut(s)	451, 1236, 1398
BsaAI	YACGTR	1 cut(s)	510
BsaHI	GRCGYC	1 cut(s)	255
BsaI	GGTCTC	1 cut(s)	89
BsaJI	CCNNGG	5 cut(s)	974, 988, 1364, 1567, 2260
BsaXI	ACNNNNNCTCC	4 cut(s)	909, 939, 2209, 2239
Bse1I	ACTGG	5 cut(s)	1450, 1732, 1798, 1970, 2257
Bse3DI	GCAATG	1 cut(s)	2164
BseBI	CCWGG	3 cut(s)	990, 1365, 1569
BseDI	CCNNGG	5 cut(s)	974, 988, 1364, 1567, 2260
BseGI	GGATG	4 cut(s)	1035, 1252, 1460, 1978
BseMI	GCAATG	1 cut(s)	2164
BseMII	CTCAG	2 cut(s)	686, 810
BseNI	ACTGG	5 cut(s)	1450, 1732, 1798, 1970, 2257
BseRI	GAGGAG	4 cut(s)	98, 215, 434, 1523
BseSI	GKGCMC	1 cut(s)	989
BseXI	GCAGC	7 cut(s)	40, 114, 497, 1020, 1123, 1570, 1613
BsgI	GTGCAG	2 cut(s)	316, 1620
Bsh1236I	CGCG	1 cut(s)	374
BshFI	GGCC	5 cut(s)	359, 670, 987, 2208, 2265
BshNI	GGYRCC	4 cut(s)	626, 682, 1207, 2079
BsiHKAI	GWGCWC	1 cut(s)	2003
BslFI	GGGAC	3 cut(s)	347, 1279, 1956
BsmAI	GTCTC	2 cut(s)	89, 912
BsmFI	GGGAC	3 cut(s)	347, 1279, 1956
BsnI	GGCC	5 cut(s)	359, 670, 987, 2208, 2265
Bso31I	GGTCTC	1 cut(s)	89
Bsp119I	TTCGAA	3 cut(s)	451, 1236, 1398
Bsp120I	GGGCCC	1 cut(s)	985
Bsp1286I	GDGCHC	2 cut(s)	989, 2003
Bsp19I	CCATGG	1 cut(s)	2260
BspACI	CCGC	6 cut(s)	369, 401, 483, 676, 679, 751
BspANI	GGCC	5 cut(s)	359, 670, 987, 2208, 2265
BspCNI	CTCAG	2 cut(s)	685, 809
BspFNI	CGCG	1 cut(s)	374
BspLI	GGNNCC	9 cut(s)	628, 684, 770, 986, 987, 1209, 1573, 1760, 2081
BspPI	GGATC	4 cut(s)	265, 841, 1693, 1946
BspQI	GCTCTTC	1 cut(s)	1132
BspT104I	TTCGAA	3 cut(s)	451, 1236, 1398
BspT107I	GGYRCC	4 cut(s)	626, 682, 1207, 2079
BspTI	CTTAAG	2 cut(s)	1375, 1610
BspTNI	GGTCTC	1 cut(s)	89
BsrDI	GCAATG	1 cut(s)	2164
BsrI	ACTGG	5 cut(s)	1450, 1732, 1798, 1970, 2257
BssECI	CCNNGG	5 cut(s)	974, 988, 1364, 1567, 2260
BssNI	GRCGYC	1 cut(s)	255
BssT1I	CCWWGG	2 cut(s)	974, 2260
Bst2UI	CCWGG	3 cut(s)	990, 1365, 1569
Bst4CI	ACNGT	6 cut(s)	472, 1190, 1672, 1851, 1909, 1923
Bst6I	CTCTTC	3 cut(s)	233, 534, 1132
BstACI	GRCGYC	1 cut(s)	255
BstAFI	CTTAAG	2 cut(s)	1375, 1610
BstAPI	GCANNNNNTGC	1 cut(s)	491
BstBAI	YACGTR	1 cut(s)	510
BstBI	TTCGAA	3 cut(s)	451, 1236, 1398
BstC8I	GCNNGC	2 cut(s)	903, 1148
BstDEI	CTNAG	5 cut(s)	34, 336, 672, 796, 1325
BstDSI	CCRYGG	1 cut(s)	2260
BstENI	CCTNNNNNAGG	1 cut(s)	1505
BstF5I	GGATG	4 cut(s)	1035, 1252, 1460, 1978
BstFNI	CGCG	1 cut(s)	374
BstH2I	RGCGCY	1 cut(s)	796
BstHHI	GCGC	2 cut(s)	374, 795
BstMAI	GTCTC	2 cut(s)	89, 912
BstMWI	GCNNNNNNNGC	4 cut(s)	25, 491, 676, 1924
BstNI	CCWGG	3 cut(s)	990, 1365, 1569
BstNSI	RCATGY	1 cut(s)	2045
BstPAI	GACNNNNGTC	1 cut(s)	2312
BstSCI	CCNGG	3 cut(s)	988, 1363, 1567
BstSLI	GKGCMC	1 cut(s)	989
BstSNI	TACGTA	1 cut(s)	510
BstUI	CGCG	1 cut(s)	374
BstV1I	GCAGC	7 cut(s)	40, 114, 497, 1020, 1123, 1570, 1613
BstV2I	GAAGAC	2 cut(s)	311, 1778
BstX2I	RGATCY	2 cut(s)	270, 833
BstXI	CCANNNNNNTGG	3 cut(s)	210, 779, 2090
BstYI	RGATCY	2 cut(s)	270, 833
BsuI	GTATCC	1 cut(s)	2249
BsuRI	GGCC	5 cut(s)	359, 670, 987, 2208, 2265
BtgI	CCRYGG	1 cut(s)	2260
BtsCI	GGATG	4 cut(s)	1035, 1252, 1460, 1978
BtsIMutI	CAGTG	3 cut(s)	804, 1791, 2250
Cac8I	GCNNGC	2 cut(s)	903, 1148
CaiI	CAGNNNCTG	1 cut(s)	47
CfoI	GCGC	2 cut(s)	374, 795
Cfr13I	GGNCC	4 cut(s)	668, 860, 985, 986
CseI	GACGC	1 cut(s)	27
Csp6I	GTAC	7 cut(s)	148, 499, 511, 544, 627, 683, 1852
CviQI	GTAC	7 cut(s)	148, 499, 511, 544, 627, 683, 1852
DdeI	CTNAG	5 cut(s)	34, 336, 672, 796, 1325
EaeI	YGGCCR	3 cut(s)	357, 2206, 2263
Eam1104I	CTCTTC	3 cut(s)	233, 534, 1132
EarI	CTCTTC	3 cut(s)	233, 534, 1132
Eco105I	TACGTA	1 cut(s)	510
Eco130I	CCWWGG	2 cut(s)	974, 2260
Eco24I	GRGCYC	1 cut(s)	989
Eco31I	GGTCTC	1 cut(s)	89
Eco47I	GGWCC	1 cut(s)	860
Eco47III	AGCGCT	1 cut(s)	794
Eco57I	CTGAAG	2 cut(s)	558, 1819
EcoNI	CCTNNNNNAGG	1 cut(s)	1505
EcoO109I	RGGNCCY	2 cut(s)	860, 985
EcoRI	GAATTC	1 cut(s)	424
EcoRII	CCWGG	3 cut(s)	988, 1363, 1567
EcoT14I	CCWWGG	2 cut(s)	974, 2260
EcoT38I	GRGCYC	1 cut(s)	989
ErhI	CCWWGG	2 cut(s)	974, 2260
FaqI	GGGAC	3 cut(s)	347, 1279, 1956
FauI	CCCGC	1 cut(s)	672
FauNDI	CATATG	1 cut(s)	1856
FbaI	TGATCA	2 cut(s)	1975, 2125
FblI	GTMKAC	3 cut(s)	1350, 1667, 1885
Fnu4HI	GCNGC	9 cut(s)	29, 128, 370, 486, 677, 1034, 1112, 1584, 1602
FokI	GGATG	4 cut(s)	1042, 1239, 1447, 1985
FriOI	GRGCYC	1 cut(s)	989
Fsp4HI	GCNGC	9 cut(s)	29, 128, 370, 486, 677, 1034, 1112, 1584, 1602
FspBI	CTAG	2 cut(s)	366, 2013
GlaI	GCGC	2 cut(s)	373, 794
GluI	GCNGC	9 cut(s)	29, 128, 370, 486, 677, 1034, 1112, 1584, 1602
GsuI	CTGGAG	2 cut(s)	1058, 1159
HaeII	RGCGCY	1 cut(s)	796
HaeIII	GGCC	5 cut(s)	359, 670, 987, 2208, 2265
HgaI	GACGC	1 cut(s)	27
HhaI	GCGC	2 cut(s)	374, 795
Hin1I	GRCGYC	1 cut(s)	255
Hin6I	GCGC	2 cut(s)	372, 793
HinP1I	GCGC	2 cut(s)	372, 793
HincII	GTYRAC	3 cut(s)	109, 995, 1090
HindII	GTYRAC	3 cut(s)	109, 995, 1090
HindIII	AAGCTT	2 cut(s)	17, 1750
HphI	GGTGA	9 cut(s)	65, 211, 395, 773, 1122, 1460, 1489, 2168, 2308
Hpy166II	GTNNAC	6 cut(s)	109, 995, 1090, 1351, 1668, 1886
Hpy188III	TCNNGA	8 cut(s)	268, 466, 518, 595, 863, 1138, 1315, 1821
Hpy8I	GTNNAC	6 cut(s)	109, 995, 1090, 1351, 1668, 1886
Hpy99I	CGWCG	2 cut(s)	257, 260
HpyAV	CCTTC	8 cut(s)	696, 954, 1005, 1220, 1224, 1933, 2183, 2208
HpyCH4III	ACNGT	6 cut(s)	472, 1190, 1672, 1851, 1909, 1923
HpyCH4IV	ACGT	5 cut(s)	255, 509, 1338, 1475, 1664
HpyF10VI	GCNNNNNNNGC	4 cut(s)	25, 491, 676, 1924
HpyF3I	CTNAG	5 cut(s)	34, 336, 672, 796, 1325
HpySE526I	ACGT	5 cut(s)	255, 509, 1338, 1475, 1664
Hsp92I	GRCGYC	1 cut(s)	255
HspAI	GCGC	2 cut(s)	372, 793
KpnI	GGTACC	2 cut(s)	630, 686
Ksp22I	TGATCA	2 cut(s)	1975, 2125
LguI	GCTCTTC	1 cut(s)	1132
LmnI	GCTCC	1 cut(s)	1510
Lsp1109I	GCAGC	7 cut(s)	40, 114, 497, 1020, 1123, 1570, 1613
LweI	GCATC	8 cut(s)	429, 565, 571, 1020, 1261, 1389, 1469, 1829
MaeI	CTAG	2 cut(s)	366, 2013
MaeII	ACGT	5 cut(s)	255, 509, 1338, 1475, 1664
MaeIII	GTNAC	5 cut(s)	609, 1288, 1495, 2305, 2314
MflI	RGATCY	2 cut(s)	270, 833
MhlI	GDGCHC	2 cut(s)	989, 2003
MlsI	TGGCCA	1 cut(s)	2265
MluNI	TGGCCA	1 cut(s)	2265
MlyI	GAGTC	8 cut(s)	273, 1053, 1261, 1296, 1369, 1610, 1733, 2322
MmeI	TCCRAC	4 cut(s)	241, 907, 1847, 1928
Mox20I	TGGCCA	1 cut(s)	2265
MroXI	GAANNNNTTC	1 cut(s)	2218
MscI	TGGCCA	1 cut(s)	2265
MslI	CAYNNNNRTG	8 cut(s)	1257, 1502, 1859, 2040, 2046, 2067, 2088, 2202
Msp20I	TGGCCA	1 cut(s)	2265
MspA1I	CMGCKG	1 cut(s)	676
MspCI	CTTAAG	2 cut(s)	1375, 1610
MspR9I	CCNGG	3 cut(s)	990, 1365, 1569
MvaI	CCWGG	3 cut(s)	990, 1365, 1569
MvnI	CGCG	1 cut(s)	374
MwoI	GCNNNNNNNGC	4 cut(s)	25, 491, 676, 1924
NcoI	CCATGG	1 cut(s)	2260
NdeI	CATATG	1 cut(s)	1856
NlaIV	GGNNCC	9 cut(s)	628, 684, 770, 986, 987, 1209, 1573, 1760, 2081
NmeAIII	GCCGAG	1 cut(s)	1669
NmuCI	GTSAC	4 cut(s)	1288, 1495, 2305, 2314
NspI	RCATGY	1 cut(s)	2045
NspV	TTCGAA	3 cut(s)	451, 1236, 1398
PciI	ACATGT	1 cut(s)	2041
PciSI	GCTCTTC	1 cut(s)	1132
PcsI	WCGNNNNNNNCGW	1 cut(s)	297
PdmI	GAANNNNTTC	1 cut(s)	2218
PfeI	GAWTC	3 cut(s)	314, 1824, 1870
PflMI	CCANNNNNTGG	2 cut(s)	1006, 1217
PkrI	GCNGC	9 cut(s)	30, 129, 371, 487, 678, 1035, 1113, 1585, 1603
PleI	GAGTC	8 cut(s)	272, 1052, 1261, 1295, 1368, 1610, 1733, 2321
PpsI	GAGTC	8 cut(s)	272, 1052, 1261, 1295, 1368, 1610, 1733, 2321
Ppu21I	YACGTR	1 cut(s)	510
PpuMI	RGGWCCY	1 cut(s)	860
PscI	ACATGT	1 cut(s)	2041
PshAI	GACNNNNGTC	1 cut(s)	2312
PshBI	ATTAAT	2 cut(s)	1302, 2025
PsiI	TTATAA	1 cut(s)	1626
Psp1406I	AACGTT	1 cut(s)	1475
Psp5II	RGGWCCY	1 cut(s)	860
Psp6I	CCWGG	3 cut(s)	988, 1363, 1567
PspGI	CCWGG	3 cut(s)	988, 1363, 1567
PspN4I	GGNNCC	9 cut(s)	628, 684, 770, 986, 987, 1209, 1573, 1760, 2081
PspOMI	GGGCCC	1 cut(s)	985
PspPI	GGNCC	4 cut(s)	668, 860, 985, 986
PspPPI	RGGWCCY	1 cut(s)	860
PsrI	GAACNNNNNNTAC	2 cut(s)	2240, 2272
PstNI	CAGNNNCTG	1 cut(s)	47
PsuI	RGATCY	2 cut(s)	270, 833
RsaI	GTAC	7 cut(s)	149, 500, 512, 545, 628, 684, 1853
RsaNI	GTAC	7 cut(s)	148, 499, 511, 544, 627, 683, 1852
RseI	CAYNNNNRTG	8 cut(s)	1257, 1502, 1859, 2040, 2046, 2067, 2088, 2202
SapI	GCTCTTC	1 cut(s)	1132
SatI	GCNGC	9 cut(s)	29, 128, 370, 486, 677, 1034, 1112, 1584, 1602
Sau96I	GGNCC	4 cut(s)	668, 860, 985, 986
ScaI	AGTACT	1 cut(s)	545
SchI	GAGTC	8 cut(s)	273, 1053, 1261, 1296, 1369, 1610, 1733, 2322
ScrFI	CCNGG	3 cut(s)	990, 1365, 1569
SduI	GDGCHC	2 cut(s)	989, 2003
SfaNI	GCATC	8 cut(s)	429, 565, 571, 1020, 1261, 1389, 1469, 1829
SfuI	TTCGAA	3 cut(s)	451, 1236, 1398
SinI	GGWCC	1 cut(s)	860
SmiMI	CAYNNNNRTG	8 cut(s)	1257, 1502, 1859, 2040, 2046, 2067, 2088, 2202
SmlI	CTYRAG	2 cut(s)	1375, 1610
SmoI	CTYRAG	2 cut(s)	1375, 1610
SnaBI	TACGTA	1 cut(s)	510
SsiI	CCGC	6 cut(s)	369, 401, 483, 676, 679, 751
SspI	AATATT	1 cut(s)	940
SspMI	CTAG	2 cut(s)	366, 2013
StyD4I	CCNGG	3 cut(s)	988, 1363, 1567
StyI	CCWWGG	2 cut(s)	974, 2260
TaaI	ACNGT	6 cut(s)	472, 1190, 1672, 1851, 1909, 1923
TaiI	ACGT	5 cut(s)	258, 512, 1341, 1478, 1667
TatI	WGTACW	2 cut(s)	543, 1851
TauI	GCSGC	2 cut(s)	372, 679
TfiI	GAWTC	3 cut(s)	314, 1824, 1870
TscAI	CASTG	3 cut(s)	804, 1798, 2257
TseFI	GTSAC	4 cut(s)	1288, 1495, 2305, 2314
TseI	GCWGC	7 cut(s)	28, 127, 485, 1033, 1111, 1583, 1601
Tsp45I	GTSAC	4 cut(s)	1288, 1495, 2305, 2314
TspRI	CASTG	3 cut(s)	804, 1798, 2257
Van91I	CCANNNNNTGG	2 cut(s)	1006, 1217
Vha464I	CTTAAG	2 cut(s)	1375, 1610
VpaK11BI	GGWCC	1 cut(s)	860
VspI	ATTAAT	2 cut(s)	1302, 2025
XagI	CCTNNNNNAGG	1 cut(s)	1505
XapI	RAATTY	4 cut(s)	424, 1003, 1165, 1539
XceI	RCATGY	1 cut(s)	2045
XcmI	CCANNNNNNNNNTGG	3 cut(s)	207, 1361, 2060
XmiI	GTMKAC	3 cut(s)	1350, 1667, 1885
XmnI	GAANNNNTTC	1 cut(s)	2218
XspI	CTAG	2 cut(s)	366, 2013
ZraI	GACGTC	1 cut(s)	256
ZrmI	AGTACT	1 cut(s)	545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.