Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

29179434 .. 29186427

Gene Structure 6994 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG162100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGGAAAATTATGGCTTATCACCCTCCTCTTCCTCCCTTGCTTCTTCATAAGTGACGACGTCGTTGGCGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTTCGAATCCGGTGACCACCTTAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGCGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCCGAAATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCACCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACGACCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTCAAGATATGTGAGAGGTTACGAGATGAAAATGGTACAATTATTGAGCATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGACGGGTACCTTCATCATGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCTAATACAGCCCATTGTGTCCGCTTTCCTAGTGATTGGTTCCATGTTTTTGGTATTGGACAGTGCTCACTGGGATTTAGTGTTCTCATTCAAGTAAAAACAGGATCTAAAGTTTCGGAAGTGCTTATAGGTCCTGAAAACAGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGACTTTGTCGGATACAAAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGGGGCCCAGGTCAACCCCAAAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAGCTATGGAATTTCTTGGAGGCTGACCAAAACCGTATTGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAACAAAGAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGCTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAATGTTAACTCGATCGTTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGAGCCGAGTGCCAATTTTCTACGACGTCTACTGTCATTGATAATGGATCACAGGGTAATCCCTTTAAGCCACCAAAGACAGGAACAACTGGTTTTTTTGACTCCATTGAAAGCTTTTGGAACCATGTGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTCGGTCTACTGTTGGCAATTTTTCCAACAGTCATTGTGCTACTGTGGCTTTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAGGATAGTCCAAGCACCAAGAATACAAGACACAGAATTAATGTAGATCACCGACATGTTCATGTCCATAAACATCATGGGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGATTACCATTATGACCTTCACCATGTCCATAAGGATAAGCACAAGCATGGCCGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCACGGCCAGAAAAAACAAAAGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

699

Amino Acids

79.92

Weight (kDa)

8.21

Isoelectric Point (pI)

40.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	45 - 557	1.9e-160	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1434
AatII	GACGTC	2 cut(s)	63, 1475
Acc65I	GGTACC	1 cut(s)	490
AccB1I	GGYRCC	3 cut(s)	490, 1015, 1875
AccB7I	CCANNNNNTGG	2 cut(s)	814, 1025
AccI	GTMKAC	3 cut(s)	1158, 1475, 1693
AccII	CGCG	1 cut(s)	182
AciI	CCGC	3 cut(s)	177, 291, 559
AclI	AACGTT	1 cut(s)	1283
AclWI	GGATC	4 cut(s)	70, 649, 1501, 1754
AcoI	YGGCCR	3 cut(s)	165, 2002, 2056
AcsI	RAATTY	3 cut(s)	232, 811, 973
AcuI	CTGAAG	2 cut(s)	366, 1627
AcyI	GRCGYC	3 cut(s)	60, 69, 1472
AfaI	GTAC	6 cut(s)	308, 320, 353, 436, 492, 1661
AfiI	CCNNNNNNNGG	7 cut(s)	163, 788, 814, 1025, 1070, 1315, 1526
AflII	CTTAAG	2 cut(s)	1183, 1418
AflIII	ACRYGT	1 cut(s)	1849
AgsI	TTSAA	9 cut(s)	237, 629, 878, 1004, 1366, 1556, 1943, 2016, 2094
AjnI	CCWGG	3 cut(s)	796, 1171, 1375
AloI	GAACNNNNNNTCC	4 cut(s)	603, 635, 1025, 1057
AluBI	AGCT	6 cut(s)	296, 713, 967, 1391, 1412, 1560
AluI	AGCT	6 cut(s)	296, 713, 967, 1391, 1412, 1560
Alw21I	GWGCWC	1 cut(s)	605
Alw26I	GTCTC	1 cut(s)	720
AlwI	GGATC	4 cut(s)	70, 649, 1501, 1754
AoxI	GGCC	6 cut(s)	165, 476, 793, 1209, 2002, 2056
ApaI	GGGCCC	1 cut(s)	797
ApeKI	GCWGC	5 cut(s)	293, 841, 919, 1391, 1409
ApoI	RAATTY	3 cut(s)	232, 811, 973
AseI	ATTAAT	2 cut(s)	1110, 1833
Asp700I	GAANNNNTTC	2 cut(s)	113, 2011
Asp718I	GGTACC	1 cut(s)	490
AspLEI	GCGC	1 cut(s)	182
AspS9I	GGNCC	5 cut(s)	476, 668, 793, 794, 1210
AsuHPI	GGTGA	8 cut(s)	13, 137, 203, 930, 1268, 1297, 1835, 1964
AsuII	TTCGAA	3 cut(s)	117, 259, 1044
AvaII	GGWCC	1 cut(s)	668
BaeGI	GKGCMC	2 cut(s)	797, 1878
BanI	GGYRCC	3 cut(s)	490, 1015, 1875
BanII	GRGCYC	1 cut(s)	797
BbsI	GAAGAC	2 cut(s)	116, 1586
Bbv12I	GWGCWC	1 cut(s)	605
BbvCI	CCTCAGC	1 cut(s)	480
BbvI	GCAGC	5 cut(s)	305, 828, 931, 1378, 1421
BccI	CCATC	1 cut(s)	863
BceAI	ACGGC	2 cut(s)	152, 2071
BcgI	CGANNNNNNTGC	4 cut(s)	239, 273, 1373, 1407
BciT130I	CCWGG	3 cut(s)	798, 1173, 1377
BciVI	GTATCC	2 cut(s)	731, 2042
BclI	TGATCA	2 cut(s)	1783, 1921
BcoDI	GTCTC	1 cut(s)	720
BfaI	CTAG	2 cut(s)	174, 567
BfrI	CTTAAG	2 cut(s)	1183, 1418
BfuI	GTATCC	2 cut(s)	731, 2042
BisI	GCNGC	6 cut(s)	178, 294, 842, 920, 1392, 1410
BlsI	GCNGC	6 cut(s)	179, 295, 843, 921, 1393, 1411
BmcAI	AGTACT	1 cut(s)	353
Bme1390I	CCNGG	3 cut(s)	798, 1173, 1377
Bme18I	GGWCC	1 cut(s)	668
BmgT120I	GGNCC	5 cut(s)	476, 668, 793, 794, 1210
BmiI	GGNNCC	8 cut(s)	492, 578, 794, 795, 1017, 1381, 1568, 1877
BmrFI	CCNGG	3 cut(s)	798, 1173, 1377
BmrI	ACTGGG	1 cut(s)	617
BmsI	GCATC	8 cut(s)	237, 373, 379, 828, 1069, 1197, 1277, 1637
BmuI	ACTGGG	1 cut(s)	617
BpiI	GAAGAC	2 cut(s)	116, 1586
BpmI	CTGGAG	2 cut(s)	866, 967
Bpu10I	CCTNAGC	1 cut(s)	480
Bpu14I	TTCGAA	3 cut(s)	117, 259, 1044
BpuEI	CTTGAG	1 cut(s)	1279
BsaAI	YACGTR	1 cut(s)	318
BsaHI	GRCGYC	3 cut(s)	60, 69, 1472
BsaJI	CCNNGG	5 cut(s)	782, 796, 1172, 1375, 2053
BsaWI	WCCGGW	1 cut(s)	122
BsaXI	ACNNNNNCTCC	4 cut(s)	717, 747, 2002, 2032
Bsc4I	CCNNNNNNNGG	7 cut(s)	163, 788, 814, 1025, 1070, 1315, 1526
Bse1I	ACTGG	6 cut(s)	612, 1258, 1540, 1606, 1778, 2050
BseBI	CCWGG	3 cut(s)	798, 1173, 1377
BseDI	CCNNGG	5 cut(s)	782, 796, 1172, 1375, 2053
BseGI	GGATG	4 cut(s)	843, 1060, 1268, 1786
BseLI	CCNNNNNNNGG	7 cut(s)	163, 788, 814, 1025, 1070, 1315, 1526
BseMII	CTCAG	1 cut(s)	494
BseNI	ACTGG	6 cut(s)	612, 1258, 1540, 1606, 1778, 2050
BseRI	GAGGAG	3 cut(s)	17, 242, 1331
BseSI	GKGCMC	2 cut(s)	797, 1878
BseXI	GCAGC	5 cut(s)	305, 828, 931, 1378, 1421
BsgI	GTGCAG	2 cut(s)	124, 1428
Bsh1236I	CGCG	1 cut(s)	182
Bsh1285I	CGRYCG	1 cut(s)	1361
BshFI	GGCC	6 cut(s)	167, 478, 795, 1211, 2004, 2058
BshNI	GGYRCC	3 cut(s)	490, 1015, 1875
BsiEI	CGRYCG	1 cut(s)	1361
BsiHKAI	GWGCWC	1 cut(s)	605
BsiSI	CCGG	1 cut(s)	123
BslFI	GGGAC	3 cut(s)	155, 1087, 1764
BslI	CCNNNNNNNGG	7 cut(s)	163, 788, 814, 1025, 1070, 1315, 1526
BsmAI	GTCTC	1 cut(s)	720
BsmFI	GGGAC	3 cut(s)	155, 1087, 1764
BsnI	GGCC	6 cut(s)	167, 478, 795, 1211, 2004, 2058
Bsp119I	TTCGAA	3 cut(s)	117, 259, 1044
Bsp120I	GGGCCC	1 cut(s)	793
Bsp1286I	GDGCHC	3 cut(s)	605, 797, 1878
BspACI	CCGC	3 cut(s)	177, 291, 559
BspANI	GGCC	6 cut(s)	167, 478, 795, 1211, 2004, 2058
BspCNI	CTCAG	1 cut(s)	493
BspFNI	CGCG	1 cut(s)	182
BspLI	GGNNCC	8 cut(s)	492, 578, 794, 795, 1017, 1381, 1568, 1877
BspPI	GGATC	4 cut(s)	70, 649, 1501, 1754
BspQI	GCTCTTC	1 cut(s)	940
BspT104I	TTCGAA	3 cut(s)	117, 259, 1044
BspT107I	GGYRCC	3 cut(s)	490, 1015, 1875
BspTI	CTTAAG	2 cut(s)	1183, 1418
BsrI	ACTGG	6 cut(s)	612, 1258, 1540, 1606, 1778, 2050
BssECI	CCNNGG	5 cut(s)	782, 796, 1172, 1375, 2053
BssNI	GRCGYC	3 cut(s)	60, 69, 1472
Bst2UI	CCWGG	3 cut(s)	798, 1173, 1377
Bst4CI	ACNGT	8 cut(s)	280, 600, 998, 1480, 1659, 1698, 1717, 1731
Bst6I	CTCTTC	3 cut(s)	35, 342, 940
BstACI	GRCGYC	3 cut(s)	60, 69, 1472
BstAFI	CTTAAG	2 cut(s)	1183, 1418
BstAPI	GCANNNNNTGC	1 cut(s)	299
BstBAI	YACGTR	1 cut(s)	318
BstBI	TTCGAA	3 cut(s)	117, 259, 1044
BstC8I	GCNNGC	2 cut(s)	711, 956
BstDEI	CTNAG	3 cut(s)	144, 480, 1133
BstDSI	CCRYGG	1 cut(s)	2053
BstEII	GGTNACC	1 cut(s)	125
BstENI	CCTNNNNNAGG	1 cut(s)	1313
BstF5I	GGATG	4 cut(s)	843, 1060, 1268, 1786
BstFNI	CGCG	1 cut(s)	182
BstHHI	GCGC	1 cut(s)	182
BstMAI	GTCTC	1 cut(s)	720
BstMCI	CGRYCG	1 cut(s)	1361
BstMWI	GCNNNNNNNGC	4 cut(s)	299, 454, 964, 1732
BstNI	CCWGG	3 cut(s)	798, 1173, 1377
BstNSI	RCATGY	1 cut(s)	1853
BstPI	GGTNACC	1 cut(s)	125
BstSCI	CCNGG	3 cut(s)	796, 1171, 1375
BstSLI	GKGCMC	2 cut(s)	797, 1878
BstSNI	TACGTA	1 cut(s)	318
BstUI	CGCG	1 cut(s)	182
BstV1I	GCAGC	5 cut(s)	305, 828, 931, 1378, 1421
BstV2I	GAAGAC	2 cut(s)	116, 1586
BstX2I	RGATCY	2 cut(s)	75, 641
BstXI	CCANNNNNNTGG	2 cut(s)	587, 1886
BstYI	RGATCY	2 cut(s)	75, 641
BsuI	GTATCC	2 cut(s)	731, 2042
BsuRI	GGCC	6 cut(s)	167, 478, 795, 1211, 2004, 2058
BtgI	CCRYGG	1 cut(s)	2053
BtsCI	GGATG	4 cut(s)	843, 1060, 1268, 1786
BtsIMutI	CAGTG	4 cut(s)	605, 605, 1599, 2043
Cac8I	GCNNGC	2 cut(s)	711, 956
CfoI	GCGC	1 cut(s)	182
Cfr13I	GGNCC	5 cut(s)	476, 668, 793, 794, 1210
CseI	GACGC	1 cut(s)	58
Csp6I	GTAC	6 cut(s)	307, 319, 352, 435, 491, 1660
CviAII	CATG	9 cut(s)	163, 501, 581, 1571, 1850, 1856, 1871, 1976, 2000
CviQI	GTAC	6 cut(s)	307, 319, 352, 435, 491, 1660
DdeI	CTNAG	3 cut(s)	144, 480, 1133
EaeI	YGGCCR	3 cut(s)	165, 2002, 2056
Eam1104I	CTCTTC	3 cut(s)	35, 342, 940
EarI	CTCTTC	3 cut(s)	35, 342, 940
Eco105I	TACGTA	1 cut(s)	318
Eco24I	GRGCYC	1 cut(s)	797
Eco47I	GGWCC	1 cut(s)	668
Eco57I	CTGAAG	2 cut(s)	366, 1627
Eco91I	GGTNACC	1 cut(s)	125
EcoNI	CCTNNNNNAGG	1 cut(s)	1313
EcoO109I	RGGNCCY	3 cut(s)	668, 793, 1210
EcoO65I	GGTNACC	1 cut(s)	125
EcoRI	GAATTC	1 cut(s)	232
EcoRII	CCWGG	3 cut(s)	796, 1171, 1375
EcoT38I	GRGCYC	1 cut(s)	797
FaeI	CATG	9 cut(s)	166, 504, 584, 1574, 1853, 1859, 1874, 1979, 2003
FaqI	GGGAC	3 cut(s)	155, 1087, 1764
FatI	CATG	9 cut(s)	162, 500, 580, 1570, 1849, 1855, 1870, 1975, 1999
FauNDI	CATATG	1 cut(s)	1664
FbaI	TGATCA	2 cut(s)	1783, 1921
FblI	GTMKAC	3 cut(s)	1158, 1475, 1693
Fnu4HI	GCNGC	6 cut(s)	178, 294, 842, 920, 1392, 1410
FokI	GGATG	4 cut(s)	850, 1047, 1255, 1793
FriOI	GRGCYC	1 cut(s)	797
Fsp4HI	GCNGC	6 cut(s)	178, 294, 842, 920, 1392, 1410
FspBI	CTAG	2 cut(s)	174, 567
GlaI	GCGC	1 cut(s)	181
GluI	GCNGC	6 cut(s)	178, 294, 842, 920, 1392, 1410
GsuI	CTGGAG	2 cut(s)	866, 967
HaeIII	GGCC	6 cut(s)	167, 478, 795, 1211, 2004, 2058
HapII	CCGG	1 cut(s)	123
HgaI	GACGC	1 cut(s)	58
HhaI	GCGC	1 cut(s)	182
Hin1I	GRCGYC	3 cut(s)	60, 69, 1472
Hin1II	CATG	9 cut(s)	166, 504, 584, 1574, 1853, 1859, 1874, 1979, 2003
Hin6I	GCGC	1 cut(s)	180
HinP1I	GCGC	1 cut(s)	180
HincII	GTYRAC	3 cut(s)	803, 898, 1353
HindII	GTYRAC	3 cut(s)	803, 898, 1353
HindIII	AAGCTT	1 cut(s)	1558
HinfI	GANTC	8 cut(s)	119, 1075, 1095, 1168, 1424, 1547, 1632, 1678
HpaI	GTTAAC	1 cut(s)	1353
HpaII	CCGG	1 cut(s)	123
HphI	GGTGA	8 cut(s)	13, 137, 203, 930, 1268, 1297, 1835, 1964
Hpy166II	GTNNAC	6 cut(s)	803, 898, 1159, 1353, 1476, 1694
Hpy188I	TCNGA	6 cut(s)	655, 737, 783, 1646, 1683, 1746
Hpy188III	TCNNGA	8 cut(s)	73, 274, 326, 403, 671, 946, 1123, 1629
Hpy8I	GTNNAC	6 cut(s)	803, 898, 1159, 1353, 1476, 1694
Hpy99I	CGWCG	4 cut(s)	62, 65, 489, 1474
HpyAV	CCTTC	8 cut(s)	504, 762, 813, 1028, 1032, 1741, 1979, 2001
HpyCH4III	ACNGT	8 cut(s)	280, 600, 998, 1480, 1659, 1698, 1717, 1731
HpyCH4IV	ACGT	5 cut(s)	60, 317, 1146, 1283, 1472
HpyF10VI	GCNNNNNNNGC	4 cut(s)	299, 454, 964, 1732
HpyF3I	CTNAG	3 cut(s)	144, 480, 1133
HpySE526I	ACGT	5 cut(s)	60, 317, 1146, 1283, 1472
Hsp92I	GRCGYC	3 cut(s)	60, 69, 1472
Hsp92II	CATG	9 cut(s)	166, 504, 584, 1574, 1853, 1859, 1874, 1979, 2003
HspAI	GCGC	1 cut(s)	180
KpnI	GGTACC	1 cut(s)	494
Ksp22I	TGATCA	2 cut(s)	1783, 1921
KspAI	GTTAAC	1 cut(s)	1353
LguI	GCTCTTC	1 cut(s)	940
LmnI	GCTCC	1 cut(s)	1318
Lsp1109I	GCAGC	5 cut(s)	305, 828, 931, 1378, 1421
LweI	GCATC	8 cut(s)	237, 373, 379, 828, 1069, 1197, 1277, 1637
MaeI	CTAG	2 cut(s)	174, 567
MaeII	ACGT	5 cut(s)	60, 317, 1146, 1283, 1472
MaeIII	GTNAC	5 cut(s)	53, 125, 417, 1096, 1303
MboII	GAAGA	8 cut(s)	22, 37, 121, 160, 256, 359, 927, 1591
MflI	RGATCY	2 cut(s)	75, 641
MhlI	GDGCHC	3 cut(s)	605, 797, 1878
MlyI	GAGTC	5 cut(s)	1069, 1104, 1177, 1418, 1541
MmeI	TCCRAC	3 cut(s)	715, 1655, 1736
MroXI	GAANNNNTTC	2 cut(s)	113, 2011
MslI	CAYNNNNRTG	7 cut(s)	1065, 1310, 1667, 1848, 1854, 1884, 1998
MspCI	CTTAAG	2 cut(s)	1183, 1418
MspI	CCGG	1 cut(s)	123
MspR9I	CCNGG	3 cut(s)	798, 1173, 1377
MvaI	CCWGG	3 cut(s)	798, 1173, 1377
MvnI	CGCG	1 cut(s)	182
MwoI	GCNNNNNNNGC	4 cut(s)	299, 454, 964, 1732
NdeI	CATATG	1 cut(s)	1664
NlaIII	CATG	9 cut(s)	166, 504, 584, 1574, 1853, 1859, 1874, 1979, 2003
NlaIV	GGNNCC	8 cut(s)	492, 578, 794, 795, 1017, 1381, 1568, 1877
NmeAIII	GCCGAG	1 cut(s)	1477
NmuCI	GTSAC	4 cut(s)	53, 125, 1096, 1303
NspI	RCATGY	1 cut(s)	1853
NspV	TTCGAA	3 cut(s)	117, 259, 1044
PciI	ACATGT	1 cut(s)	1849
PciSI	GCTCTTC	1 cut(s)	940
PcsI	WCGNNNNNNNCGW	2 cut(s)	66, 102
PdmI	GAANNNNTTC	2 cut(s)	113, 2011
PfeI	GAWTC	3 cut(s)	119, 1632, 1678
PflFI	GACNNNGTC	2 cut(s)	59, 731
PflMI	CCANNNNNTGG	2 cut(s)	814, 1025
PkrI	GCNGC	6 cut(s)	179, 295, 843, 921, 1393, 1411
Ple19I	CGATCG	1 cut(s)	1361
PleI	GAGTC	5 cut(s)	1069, 1103, 1176, 1418, 1541
PpsI	GAGTC	5 cut(s)	1069, 1103, 1176, 1418, 1541
Ppu21I	YACGTR	1 cut(s)	318
PpuMI	RGGWCCY	1 cut(s)	668
PscI	ACATGT	1 cut(s)	1849
PshBI	ATTAAT	2 cut(s)	1110, 1833
PsiI	TTATAA	1 cut(s)	1434
Psp1406I	AACGTT	1 cut(s)	1283
Psp5II	RGGWCCY	1 cut(s)	668
Psp6I	CCWGG	3 cut(s)	796, 1171, 1375
PspEI	GGTNACC	1 cut(s)	125
PspGI	CCWGG	3 cut(s)	796, 1171, 1375
PspN4I	GGNNCC	8 cut(s)	492, 578, 794, 795, 1017, 1381, 1568, 1877
PspOMI	GGGCCC	1 cut(s)	793
PspPI	GGNCC	5 cut(s)	476, 668, 793, 794, 1210
PspPPI	RGGWCCY	1 cut(s)	668
PsrI	GAACNNNNNNTAC	2 cut(s)	2033, 2065
PsuI	RGATCY	2 cut(s)	75, 641
PsyI	GACNNNGTC	2 cut(s)	59, 731
PvuI	CGATCG	1 cut(s)	1361
RsaI	GTAC	6 cut(s)	308, 320, 353, 436, 492, 1661
RsaNI	GTAC	6 cut(s)	307, 319, 352, 435, 491, 1660
RseI	CAYNNNNRTG	7 cut(s)	1065, 1310, 1667, 1848, 1854, 1884, 1998
SapI	GCTCTTC	1 cut(s)	940
SatI	GCNGC	6 cut(s)	178, 294, 842, 920, 1392, 1410
Sau96I	GGNCC	5 cut(s)	476, 668, 793, 794, 1210
ScaI	AGTACT	1 cut(s)	353
SchI	GAGTC	5 cut(s)	1069, 1104, 1177, 1418, 1541
ScrFI	CCNGG	3 cut(s)	798, 1173, 1377
SduI	GDGCHC	3 cut(s)	605, 797, 1878
SfaNI	GCATC	8 cut(s)	237, 373, 379, 828, 1069, 1197, 1277, 1637
SfuI	TTCGAA	3 cut(s)	117, 259, 1044
SinI	GGWCC	1 cut(s)	668
SmiMI	CAYNNNNRTG	7 cut(s)	1065, 1310, 1667, 1848, 1854, 1884, 1998
SmlI	CTYRAG	3 cut(s)	1183, 1294, 1418
SmoI	CTYRAG	3 cut(s)	1183, 1294, 1418
SnaBI	TACGTA	1 cut(s)	318
SsiI	CCGC	3 cut(s)	177, 291, 559
SspI	AATATT	1 cut(s)	748
SspMI	CTAG	2 cut(s)	174, 567
StyD4I	CCNGG	3 cut(s)	796, 1171, 1375
TaaI	ACNGT	8 cut(s)	280, 600, 998, 1480, 1659, 1698, 1717, 1731
TaiI	ACGT	5 cut(s)	63, 320, 1149, 1286, 1475
TaqII	GACCGA	1 cut(s)	1679
TatI	WGTACW	2 cut(s)	351, 1659
TauI	GCSGC	1 cut(s)	180
TfiI	GAWTC	3 cut(s)	119, 1632, 1678
TscAI	CASTG	4 cut(s)	605, 612, 1606, 2050
TseFI	GTSAC	4 cut(s)	53, 125, 1096, 1303
TseI	GCWGC	5 cut(s)	293, 841, 919, 1391, 1409
Tsp45I	GTSAC	4 cut(s)	53, 125, 1096, 1303
TspRI	CASTG	4 cut(s)	605, 612, 1606, 2050
Tth111I	GACNNNGTC	2 cut(s)	59, 731
Van91I	CCANNNNNTGG	2 cut(s)	814, 1025
Vha464I	CTTAAG	2 cut(s)	1183, 1418
VpaK11BI	GGWCC	1 cut(s)	668
VspI	ATTAAT	2 cut(s)	1110, 1833
XagI	CCTNNNNNAGG	1 cut(s)	1313
XapI	RAATTY	3 cut(s)	232, 811, 973
XceI	RCATGY	1 cut(s)	1853
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1169, 1868
XmiI	GTMKAC	3 cut(s)	1158, 1475, 1693
XmnI	GAANNNNTTC	2 cut(s)	113, 2011
XspI	CTAG	2 cut(s)	174, 567
ZraI	GACGTC	2 cut(s)	61, 1473
ZrmI	AGTACT	1 cut(s)	353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.