Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

31621572 .. 31628402

Gene Structure 6831 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG158600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGAGGTTCTTCAAGCTTGTTCAGCAGCACTTGGCGTCAGGCTCTGATGGTGAAGATAATGGAAGTTCAAAGATTTCAGAGGAGGATTTGGAGACCCCATTTGTTGACTTCTTACAGACACGGCTGCCTCCCAAGATAAAATCGTACAGATCATATCGCCTTTCTCTATCTACATTCCCAAAAATGGGAAACCCTCCACCAAAATTATGGCTTATCACCCTCCTCTTCCTCCCTTGCTTCTTCATAAGCGACGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTCCGAATCCGATGAACTTAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGTGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAAATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCACCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACCAGCTTACGTACATTAGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTCAAGATATGTGAGAGGTTACGAGATGAAAATGGTACCATTATTGAACATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCTTGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCGAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATCGGACAACGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGGATCTAAAGTTTCGGAAGTGCTTGTAGGTCCTGAAAACAGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGACTTTGTTGGATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAATTATGGAATTTCCTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAAAATACTGGTGAAGTAGAAGCATCCTATTGCTTAACGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAAGATAGTCCGAGCACCAAGAATACTAGACACAGAATTAATGTAGATCATCAACATGTTCATGTCCATAAACATCATGGGCATGAAACAAGGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGATTACCATTACGACCTTCACCATGTCCATAAGGATAAGCACAAGCATAGCCGAAGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCACGGCCAGAAAAAACAAAGGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

581

Amino Acids

66.26

Weight (kDa)

8.73

Isoelectric Point (pI)

42.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	108 - 315	5.5e-56	Male gamete fusion factor
HAP2-GCS1	PF10699	339 - 459	3.2e-39	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	255
Acc65I	GGTACC	2 cut(s)	623, 679
AccB1I	GGYRCC	4 cut(s)	623, 679, 1108, 1518
AccB7I	CCANNNNNTGG	2 cut(s)	995, 1118
AccI	GTMKAC	1 cut(s)	1251
AccII	CGCG	1 cut(s)	371
AciI	CCGC	5 cut(s)	398, 480, 673, 676, 748
AclWI	GGATC	3 cut(s)	262, 838, 1385
AcoI	YGGCCR	2 cut(s)	354, 1702
AcsI	RAATTY	3 cut(s)	421, 992, 1066
AcuI	CTGAAG	1 cut(s)	555
AcyI	GRCGYC	2 cut(s)	35, 252
AfaI	GTAC	6 cut(s)	146, 497, 509, 542, 625, 681
AfiI	CCNNNNNNNGG	8 cut(s)	184, 185, 352, 754, 969, 995, 1118, 1163
AflII	CTTAAG	1 cut(s)	1276
AflIII	ACRYGT	1 cut(s)	1480
AgsI	TTSAA	8 cut(s)	13, 69, 426, 635, 818, 1586, 1662, 1740
AjnI	CCWGG	3 cut(s)	977, 1071, 1264
AloI	GAACNNNNNNTCC	4 cut(s)	792, 824, 1118, 1150
AluBI	AGCT	4 cut(s)	16, 485, 502, 902
AluI	AGCT	4 cut(s)	16, 485, 502, 902
Alw21I	GWGCWC	1 cut(s)	1442
Alw26I	GTCTC	2 cut(s)	86, 909
AlwI	GGATC	3 cut(s)	262, 838, 1385
AlwNI	CAGNNNCTG	1 cut(s)	44
AoxI	GGCC	5 cut(s)	354, 665, 974, 1302, 1702
ApaI	GGGCCC	1 cut(s)	978
ApeKI	GCWGC	3 cut(s)	25, 124, 482
ApoI	RAATTY	3 cut(s)	421, 992, 1066
AseI	ATTAAT	2 cut(s)	1203, 1464
Asp700I	GAANNNNTTC	1 cut(s)	1657
Asp718I	GGTACC	2 cut(s)	623, 679
AspLEI	GCGC	1 cut(s)	371
AspS9I	GGNCC	5 cut(s)	665, 857, 974, 975, 1303
AsuC2I	CCSGG	1 cut(s)	756
AsuHPI	GGTGA	7 cut(s)	62, 208, 392, 770, 1023, 1361, 1607
AsuII	TTCGAA	2 cut(s)	448, 1137
AvaII	GGWCC	1 cut(s)	857
BaeGI	GKGCMC	1 cut(s)	978
BanI	GGYRCC	4 cut(s)	623, 679, 1108, 1518
BanII	GRGCYC	1 cut(s)	978
BbsI	GAAGAC	1 cut(s)	308
Bbv12I	GWGCWC	1 cut(s)	1442
BbvCI	CCTCAGC	1 cut(s)	669
BbvI	GCAGC	3 cut(s)	37, 111, 494
BccI	CCATC	1 cut(s)	41
BceAI	ACGGC	3 cut(s)	137, 341, 1717
BcgI	CGANNNNNNTGC	2 cut(s)	428, 462
BciT130I	CCWGG	3 cut(s)	979, 1073, 1266
BciVI	GTATCC	1 cut(s)	1688
BclI	TGATCA	2 cut(s)	1414, 1564
BcnI	CCSGG	1 cut(s)	756
BcoDI	GTCTC	2 cut(s)	86, 909
BfaI	CTAG	2 cut(s)	363, 1452
BfrI	CTTAAG	1 cut(s)	1276
BfuI	GTATCC	1 cut(s)	1688
BglI	GCCNNNNNGGC	1 cut(s)	673
BisI	GCNGC	4 cut(s)	26, 125, 483, 674
BlsI	GCNGC	4 cut(s)	27, 126, 484, 675
BmcAI	AGTACT	1 cut(s)	542
Bme1390I	CCNGG	4 cut(s)	756, 979, 1073, 1266
Bme18I	GGWCC	1 cut(s)	857
BmgT120I	GGNCC	5 cut(s)	665, 857, 974, 975, 1303
BmiI	GGNNCC	7 cut(s)	625, 681, 767, 975, 976, 1110, 1520
BmrFI	CCNGG	4 cut(s)	756, 979, 1073, 1266
BmsI	GCATC	6 cut(s)	426, 562, 568, 1162, 1290, 1370
BpiI	GAAGAC	1 cut(s)	308
BpmI	CTGGAG	2 cut(s)	1060, 1094
Bpu10I	CCTNAGC	1 cut(s)	669
Bpu14I	TTCGAA	2 cut(s)	448, 1137
BpuMI	CCSGG	1 cut(s)	756
BsaAI	YACGTR	1 cut(s)	507
BsaHI	GRCGYC	2 cut(s)	35, 252
BsaI	GGTCTC	1 cut(s)	86
BsaJI	CCNNGG	4 cut(s)	963, 977, 1265, 1699
BsaXI	ACNNNNNCTCC	4 cut(s)	906, 936, 1648, 1678
Bsc4I	CCNNNNNNNGG	8 cut(s)	184, 185, 352, 754, 969, 995, 1118, 1163
Bse1I	ACTGG	3 cut(s)	1351, 1409, 1696
BseBI	CCWGG	3 cut(s)	979, 1073, 1266
BseDI	CCNNGG	4 cut(s)	963, 977, 1265, 1699
BseGI	GGATG	3 cut(s)	1153, 1361, 1417
BseLI	CCNNNNNNNGG	8 cut(s)	184, 185, 352, 754, 969, 995, 1118, 1163
BseMII	CTCAG	2 cut(s)	683, 807
BseNI	ACTGG	3 cut(s)	1351, 1409, 1696
BseRI	GAGGAG	3 cut(s)	95, 212, 431
BseSI	GKGCMC	1 cut(s)	978
BseXI	GCAGC	3 cut(s)	37, 111, 494
BsgI	GTGCAG	1 cut(s)	313
Bsh1236I	CGCG	1 cut(s)	371
BshFI	GGCC	5 cut(s)	356, 667, 976, 1304, 1704
BshNI	GGYRCC	4 cut(s)	623, 679, 1108, 1518
BsiHKAI	GWGCWC	1 cut(s)	1442
BsiSI	CCGG	1 cut(s)	755
BslFI	GGGAC	3 cut(s)	344, 1180, 1395
BslI	CCNNNNNNNGG	8 cut(s)	184, 185, 352, 754, 969, 995, 1118, 1163
BsmAI	GTCTC	2 cut(s)	86, 909
BsmFI	GGGAC	3 cut(s)	344, 1180, 1395
BsnI	GGCC	5 cut(s)	356, 667, 976, 1304, 1704
Bso31I	GGTCTC	1 cut(s)	86
Bsp119I	TTCGAA	2 cut(s)	448, 1137
Bsp120I	GGGCCC	1 cut(s)	974
Bsp1286I	GDGCHC	2 cut(s)	978, 1442
Bsp143I	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
BspACI	CCGC	5 cut(s)	398, 480, 673, 676, 748
BspANI	GGCC	5 cut(s)	356, 667, 976, 1304, 1704
BspCNI	CTCAG	2 cut(s)	682, 806
BspFNI	CGCG	1 cut(s)	371
BspLI	GGNNCC	7 cut(s)	625, 681, 767, 975, 976, 1110, 1520
BspPI	GGATC	3 cut(s)	262, 838, 1385
BspQI	GCTCTTC	1 cut(s)	1033
BspT104I	TTCGAA	2 cut(s)	448, 1137
BspT107I	GGYRCC	4 cut(s)	623, 679, 1108, 1518
BspTI	CTTAAG	1 cut(s)	1276
BspTNI	GGTCTC	1 cut(s)	86
BsrI	ACTGG	3 cut(s)	1351, 1409, 1696
BssECI	CCNNGG	4 cut(s)	963, 977, 1265, 1699
BssMI	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
BssNI	GRCGYC	2 cut(s)	35, 252
Bst2UI	CCWGG	3 cut(s)	979, 1073, 1266
Bst4CI	ACNGT	2 cut(s)	469, 1091
Bst6I	CTCTTC	3 cut(s)	230, 531, 1033
BstACI	GRCGYC	2 cut(s)	35, 252
BstAFI	CTTAAG	1 cut(s)	1276
BstAPI	GCANNNNNTGC	1 cut(s)	488
BstBAI	YACGTR	1 cut(s)	507
BstBI	TTCGAA	2 cut(s)	448, 1137
BstC8I	GCNNGC	2 cut(s)	900, 1049
BstDEI	CTNAG	4 cut(s)	333, 669, 793, 1226
BstDSI	CCRYGG	1 cut(s)	1699
BstF5I	GGATG	3 cut(s)	1153, 1361, 1417
BstFNI	CGCG	1 cut(s)	371
BstHHI	GCGC	1 cut(s)	371
BstKTI	GATC	8 cut(s)	152, 270, 342, 833, 1393, 1417, 1474, 1567
BstMAI	GTCTC	2 cut(s)	86, 909
BstMBI	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
BstMWI	GCNNNNNNNGC	3 cut(s)	22, 488, 673
BstNI	CCWGG	3 cut(s)	979, 1073, 1266
BstNSI	RCATGY	1 cut(s)	1484
BstSCI	CCNGG	4 cut(s)	754, 977, 1071, 1264
BstSLI	GKGCMC	1 cut(s)	978
BstSNI	TACGTA	1 cut(s)	507
BstUI	CGCG	1 cut(s)	371
BstV1I	GCAGC	3 cut(s)	37, 111, 494
BstV2I	GAAGAC	1 cut(s)	308
BstX2I	RGATCY	2 cut(s)	267, 830
BstXI	CCANNNNNNTGG	3 cut(s)	207, 776, 1529
BstYI	RGATCY	2 cut(s)	267, 830
BsuI	GTATCC	1 cut(s)	1688
BsuRI	GGCC	5 cut(s)	356, 667, 976, 1304, 1704
BtgI	CCRYGG	1 cut(s)	1699
BtsCI	GGATG	3 cut(s)	1153, 1361, 1417
BtsIMutI	CAGTG	2 cut(s)	801, 1689
Cac8I	GCNNGC	2 cut(s)	900, 1049
CaiI	CAGNNNCTG	1 cut(s)	44
CfoI	GCGC	1 cut(s)	371
Cfr13I	GGNCC	5 cut(s)	665, 857, 974, 975, 1303
CseI	GACGC	1 cut(s)	24
Csp6I	GTAC	6 cut(s)	145, 496, 508, 541, 624, 680
CviAII	CATG	7 cut(s)	352, 770, 1481, 1487, 1502, 1508, 1619
CviQI	GTAC	6 cut(s)	145, 496, 508, 541, 624, 680
DdeI	CTNAG	4 cut(s)	333, 669, 793, 1226
DpnI	GATC	8 cut(s)	151, 269, 341, 832, 1392, 1416, 1473, 1566
DpnII	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
EaeI	YGGCCR	2 cut(s)	354, 1702
Eam1104I	CTCTTC	3 cut(s)	230, 531, 1033
EarI	CTCTTC	3 cut(s)	230, 531, 1033
Eco105I	TACGTA	1 cut(s)	507
Eco24I	GRGCYC	1 cut(s)	978
Eco31I	GGTCTC	1 cut(s)	86
Eco47I	GGWCC	1 cut(s)	857
Eco57I	CTGAAG	1 cut(s)	555
EcoO109I	RGGNCCY	3 cut(s)	857, 974, 1303
EcoRI	GAATTC	1 cut(s)	421
EcoRII	CCWGG	3 cut(s)	977, 1071, 1264
EcoT38I	GRGCYC	1 cut(s)	978
FaeI	CATG	7 cut(s)	355, 773, 1484, 1490, 1505, 1511, 1622
FaqI	GGGAC	3 cut(s)	344, 1180, 1395
FatI	CATG	7 cut(s)	351, 769, 1480, 1486, 1501, 1507, 1618
FauI	CCCGC	1 cut(s)	669
FbaI	TGATCA	2 cut(s)	1414, 1564
FblI	GTMKAC	1 cut(s)	1251
Fnu4HI	GCNGC	4 cut(s)	26, 125, 483, 674
FokI	GGATG	3 cut(s)	1140, 1348, 1424
FriOI	GRGCYC	1 cut(s)	978
Fsp4HI	GCNGC	4 cut(s)	26, 125, 483, 674
FspBI	CTAG	2 cut(s)	363, 1452
GlaI	GCGC	1 cut(s)	370
GluI	GCNGC	4 cut(s)	26, 125, 483, 674
GsuI	CTGGAG	2 cut(s)	1060, 1094
HaeIII	GGCC	5 cut(s)	356, 667, 976, 1304, 1704
HapII	CCGG	1 cut(s)	755
HgaI	GACGC	1 cut(s)	24
HhaI	GCGC	1 cut(s)	371
Hin1I	GRCGYC	2 cut(s)	35, 252
Hin1II	CATG	7 cut(s)	355, 773, 1484, 1490, 1505, 1511, 1622
Hin6I	GCGC	1 cut(s)	369
HinP1I	GCGC	1 cut(s)	369
HincII	GTYRAC	2 cut(s)	106, 984
HindII	GTYRAC	2 cut(s)	106, 984
HindIII	AAGCTT	1 cut(s)	14
HinfI	GANTC	5 cut(s)	261, 311, 1168, 1188, 1261
HpaII	CCGG	1 cut(s)	755
HphI	GGTGA	7 cut(s)	62, 208, 392, 770, 1023, 1361, 1607
Hpy166II	GTNNAC	3 cut(s)	106, 984, 1252
Hpy188I	TCNGA	8 cut(s)	46, 79, 310, 316, 785, 844, 964, 1437
Hpy188III	TCNNGA	6 cut(s)	265, 463, 592, 860, 1039, 1216
Hpy8I	GTNNAC	3 cut(s)	106, 984, 1252
Hpy99I	CGWCG	2 cut(s)	254, 257
HpyAV	CCTTC	8 cut(s)	693, 943, 994, 1121, 1125, 1372, 1622, 1647
HpyCH4III	ACNGT	2 cut(s)	469, 1091
HpyCH4IV	ACGT	3 cut(s)	252, 506, 1239
HpyCH4V	TGCA	8 cut(s)	330, 439, 524, 575, 581, 1051, 1163, 1672
HpyF10VI	GCNNNNNNNGC	3 cut(s)	22, 488, 673
HpyF3I	CTNAG	4 cut(s)	333, 669, 793, 1226
HpySE526I	ACGT	3 cut(s)	252, 506, 1239
Hsp92I	GRCGYC	2 cut(s)	35, 252
Hsp92II	CATG	7 cut(s)	355, 773, 1484, 1490, 1505, 1511, 1622
HspAI	GCGC	1 cut(s)	369
KpnI	GGTACC	2 cut(s)	627, 683
Ksp22I	TGATCA	2 cut(s)	1414, 1564
Kzo9I	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
LguI	GCTCTTC	1 cut(s)	1033
Lsp1109I	GCAGC	3 cut(s)	37, 111, 494
LweI	GCATC	6 cut(s)	426, 562, 568, 1162, 1290, 1370
MaeI	CTAG	2 cut(s)	363, 1452
MaeII	ACGT	3 cut(s)	252, 506, 1239
MaeIII	GTNAC	2 cut(s)	606, 1189
MalI	GATC	8 cut(s)	151, 269, 341, 832, 1392, 1416, 1473, 1566
MboI	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
MflI	RGATCY	2 cut(s)	267, 830
MhlI	GDGCHC	2 cut(s)	978, 1442
MluCI	AATT	9 cut(s)	203, 402, 421, 958, 992, 1058, 1066, 1313, 1461
MlyI	GAGTC	4 cut(s)	270, 1162, 1197, 1270
MmeI	TCCRAC	2 cut(s)	238, 904
MroXI	GAANNNNTTC	1 cut(s)	1657
MseI	TTAA	6 cut(s)	324, 549, 1203, 1277, 1373, 1464
MslI	CAYNNNNRTG	5 cut(s)	1158, 1479, 1485, 1506, 1527
MspA1I	CMGCKG	1 cut(s)	673
MspCI	CTTAAG	1 cut(s)	1276
MspI	CCGG	1 cut(s)	755
MspR9I	CCNGG	4 cut(s)	756, 979, 1073, 1266
MvaI	CCWGG	3 cut(s)	979, 1073, 1266
MvnI	CGCG	1 cut(s)	371
MwoI	GCNNNNNNNGC	3 cut(s)	22, 488, 673
NciI	CCSGG	1 cut(s)	756
NdeII	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
NlaIII	CATG	7 cut(s)	355, 773, 1484, 1490, 1505, 1511, 1622
NlaIV	GGNNCC	7 cut(s)	625, 681, 767, 975, 976, 1110, 1520
NmuCI	GTSAC	1 cut(s)	1189
NspI	RCATGY	1 cut(s)	1484
NspV	TTCGAA	2 cut(s)	448, 1137
PciI	ACATGT	1 cut(s)	1480
PciSI	GCTCTTC	1 cut(s)	1033
PcsI	WCGNNNNNNNCGW	1 cut(s)	294
PdmI	GAANNNNTTC	1 cut(s)	1657
PfeI	GAWTC	1 cut(s)	311
PflMI	CCANNNNNTGG	2 cut(s)	995, 1118
PfoI	TCCNGGA	1 cut(s)	1071
PkrI	GCNGC	4 cut(s)	27, 126, 484, 675
PleI	GAGTC	4 cut(s)	269, 1162, 1196, 1269
PpsI	GAGTC	4 cut(s)	269, 1162, 1196, 1269
Ppu21I	YACGTR	1 cut(s)	507
PpuMI	RGGWCCY	1 cut(s)	857
PscI	ACATGT	1 cut(s)	1480
PshBI	ATTAAT	2 cut(s)	1203, 1464
Psp5II	RGGWCCY	1 cut(s)	857
Psp6I	CCWGG	3 cut(s)	977, 1071, 1264
PspGI	CCWGG	3 cut(s)	977, 1071, 1264
PspN4I	GGNNCC	7 cut(s)	625, 681, 767, 975, 976, 1110, 1520
PspOMI	GGGCCC	1 cut(s)	974
PspPI	GGNCC	5 cut(s)	665, 857, 974, 975, 1303
PspPPI	RGGWCCY	1 cut(s)	857
PsrI	GAACNNNNNNTAC	2 cut(s)	1679, 1711
PstNI	CAGNNNCTG	1 cut(s)	44
PsuI	RGATCY	2 cut(s)	267, 830
RsaI	GTAC	6 cut(s)	146, 497, 509, 542, 625, 681
RsaNI	GTAC	6 cut(s)	145, 496, 508, 541, 624, 680
RseI	CAYNNNNRTG	5 cut(s)	1158, 1479, 1485, 1506, 1527
SapI	GCTCTTC	1 cut(s)	1033
SaqAI	TTAA	6 cut(s)	324, 549, 1203, 1277, 1373, 1464
SatI	GCNGC	4 cut(s)	26, 125, 483, 674
Sau3AI	GATC	8 cut(s)	149, 267, 339, 830, 1390, 1414, 1471, 1564
Sau96I	GGNCC	5 cut(s)	665, 857, 974, 975, 1303
ScaI	AGTACT	1 cut(s)	542
SchI	GAGTC	4 cut(s)	270, 1162, 1197, 1270
ScrFI	CCNGG	4 cut(s)	756, 979, 1073, 1266
SduI	GDGCHC	2 cut(s)	978, 1442
SfaNI	GCATC	6 cut(s)	426, 562, 568, 1162, 1290, 1370
SfuI	TTCGAA	2 cut(s)	448, 1137
SinI	GGWCC	1 cut(s)	857
SmiMI	CAYNNNNRTG	5 cut(s)	1158, 1479, 1485, 1506, 1527
SmlI	CTYRAG	1 cut(s)	1276
SmoI	CTYRAG	1 cut(s)	1276
SnaBI	TACGTA	1 cut(s)	507
Sse9I	AATT	9 cut(s)	203, 402, 421, 958, 992, 1058, 1066, 1313, 1461
SsiI	CCGC	5 cut(s)	398, 480, 673, 676, 748
SspMI	CTAG	2 cut(s)	363, 1452
StyD4I	CCNGG	4 cut(s)	754, 977, 1071, 1264
TaaI	ACNGT	2 cut(s)	469, 1091
TaiI	ACGT	3 cut(s)	255, 509, 1242
TaqI	TCGA	8 cut(s)	281, 288, 389, 408, 448, 940, 1095, 1137
TasI	AATT	9 cut(s)	203, 402, 421, 958, 992, 1058, 1066, 1313, 1461
TatI	WGTACW	1 cut(s)	540
TauI	GCSGC	1 cut(s)	676
TfiI	GAWTC	1 cut(s)	311
Tru1I	TTAA	6 cut(s)	324, 549, 1203, 1277, 1373, 1464
Tru9I	TTAA	6 cut(s)	324, 549, 1203, 1277, 1373, 1464
TscAI	CASTG	2 cut(s)	801, 1696
TseFI	GTSAC	1 cut(s)	1189
TseI	GCWGC	3 cut(s)	25, 124, 482
Tsp45I	GTSAC	1 cut(s)	1189
TspDTI	ATGAA	8 cut(s)	232, 333, 630, 675, 731, 925, 1475, 1524
TspRI	CASTG	2 cut(s)	801, 1696
Van91I	CCANNNNNTGG	2 cut(s)	995, 1118
Vha464I	CTTAAG	1 cut(s)	1276
VpaK11BI	GGWCC	1 cut(s)	857
VspI	ATTAAT	2 cut(s)	1203, 1464
XapI	RAATTY	3 cut(s)	421, 992, 1066
XceI	RCATGY	1 cut(s)	1484
XcmI	CCANNNNNNNNNTGG	3 cut(s)	204, 1262, 1499
XmiI	GTMKAC	1 cut(s)	1251
XmnI	GAANNNNTTC	1 cut(s)	1657
XspI	CTAG	2 cut(s)	363, 1452
ZraI	GACGTC	1 cut(s)	253
ZrmI	AGTACT	1 cut(s)	542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.