Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

34581809 .. 34588298

Gene Structure 6490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ38165

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGGGAAACCCTCCACCAAAATTATGGCTTATCACCCTCCTCTTCCTCCCTTGCTTCTTCATAAGCGACGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTTGACAAGTGCGAGAAGACTTCCGAATCCGGTGAACTTAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGCGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAAATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCACCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACGAGCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCATGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCGAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGCATCCAAAGTTTCGGAAGTGATTGTAGGTCCTGAAAACAGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGATTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCTAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAATTATGGAATTTCTTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAAAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGGAGCAATACTTCATTATGAAGCCAAAAGAAATGTTAACTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGAGCCGAGTGCCAATTTTCTACGACGTCTACTGTCATTGATAATGGATCACAGGTTAATCCCTTTAAGCCACCAAAGACAGGAACAACTGGTTTCTTTGACTCCATTGAAAGCTTTTGGAACCATGCGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTTGGTCTACTTTTGGCAATTTTTCCAACAGTCATTGTGCTACTGTGGCTTTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAGGATAGTCCGAGCACCAAGAATACTAGACACAGAATTAATGTAGATCATCGACATGTTCATGTCCATAAACATCATGGGCATGAAACAATGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGATTACAATTACGACCTTCACCATGTCCATAAGGATAAGCACAAGCATGGCCGAAGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCATGGCCAGAAAAAACAAAGGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

676

Amino Acids

77.2

Weight (kDa)

8.72

Isoelectric Point (pI)

41.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	47 - 529	1.3e-142	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1350
AatII	GACGTC	2 cut(s)	72, 1391
Acc65I	GGTACC	1 cut(s)	439
AccB1I	GGYRCC	2 cut(s)	439, 964
AccB7I	CCANNNNNTGG	2 cut(s)	763, 974
AccI	GTMKAC	3 cut(s)	1107, 1391, 1609
AccII	CGCG	1 cut(s)	188
AciI	CCGC	6 cut(s)	183, 215, 297, 433, 436, 508
AclWI	GGATC	3 cut(s)	79, 1417, 1670
AcoI	YGGCCR	3 cut(s)	171, 1930, 1987
AcsI	RAATTY	3 cut(s)	238, 760, 922
AcuI	CTGAAG	2 cut(s)	372, 1543
AcyI	GRCGYC	2 cut(s)	69, 1388
AfaI	GTAC	5 cut(s)	314, 326, 359, 441, 1577
AfeI	AGCGCT	1 cut(s)	551
AfiI	CCNNNNNNNGG	7 cut(s)	169, 514, 737, 763, 974, 1019, 1442
AflII	CTTAAG	2 cut(s)	1132, 1334
AflIII	ACRYGT	1 cut(s)	1765
AgsI	TTSAA	8 cut(s)	243, 578, 827, 1282, 1472, 1871, 1947, 2025
AjnI	CCWGG	3 cut(s)	745, 1120, 1291
AloI	GAACNNNNNNTCC	4 cut(s)	552, 584, 974, 1006
AluBI	AGCT	6 cut(s)	302, 319, 662, 1307, 1328, 1476
AluI	AGCT	6 cut(s)	302, 319, 662, 1307, 1328, 1476
Alw21I	GWGCWC	1 cut(s)	1727
AlwI	GGATC	3 cut(s)	79, 1417, 1670
Aor51HI	AGCGCT	1 cut(s)	551
AoxI	GGCC	6 cut(s)	171, 425, 742, 1158, 1930, 1987
ApaI	GGGCCC	1 cut(s)	746
ApeKI	GCWGC	5 cut(s)	299, 790, 868, 1307, 1325
ApoI	RAATTY	3 cut(s)	238, 760, 922
AseI	ATTAAT	2 cut(s)	1059, 1749
Asp700I	GAANNNNTTC	1 cut(s)	1942
Asp718I	GGTACC	1 cut(s)	439
AspA2I	CCTAGG	1 cut(s)	731
AspLEI	GCGC	2 cut(s)	188, 552
AspS9I	GGNCC	5 cut(s)	425, 617, 742, 743, 1159
AsuC2I	CCSGG	1 cut(s)	516
AsuHPI	GGTGA	7 cut(s)	25, 146, 209, 530, 879, 1217, 1892
AsuII	TTCGAA	2 cut(s)	265, 993
AvaII	GGWCC	1 cut(s)	617
AvrII	CCTAGG	1 cut(s)	731
BaeGI	GKGCMC	1 cut(s)	746
BalI	TGGCCA	1 cut(s)	1989
BanI	GGYRCC	2 cut(s)	439, 964
BanII	GRGCYC	1 cut(s)	746
BbsI	GAAGAC	2 cut(s)	125, 1502
Bbv12I	GWGCWC	1 cut(s)	1727
BbvCI	CCTCAGC	1 cut(s)	429
BbvI	GCAGC	5 cut(s)	311, 777, 880, 1294, 1337
BccI	CCATC	1 cut(s)	812
BceAI	ACGGC	1 cut(s)	158
BcgI	CGANNNNNNTGC	4 cut(s)	245, 279, 1289, 1323
BciT130I	CCWGG	3 cut(s)	747, 1122, 1293
BciVI	GTATCC	2 cut(s)	680, 1973
BclI	TGATCA	2 cut(s)	1699, 1849
BcnI	CCSGG	1 cut(s)	516
BfaI	CTAG	3 cut(s)	180, 732, 1737
BfoI	RGCGCY	1 cut(s)	553
BfrI	CTTAAG	2 cut(s)	1132, 1334
BfuI	GTATCC	2 cut(s)	680, 1973
BglI	GCCNNNNNGGC	1 cut(s)	433
BisI	GCNGC	7 cut(s)	184, 300, 434, 791, 869, 1308, 1326
BlnI	CCTAGG	1 cut(s)	731
BlsI	GCNGC	7 cut(s)	185, 301, 435, 792, 870, 1309, 1327
BmcAI	AGTACT	1 cut(s)	359
Bme1390I	CCNGG	4 cut(s)	516, 747, 1122, 1293
Bme18I	GGWCC	1 cut(s)	617
BmgT120I	GGNCC	5 cut(s)	425, 617, 742, 743, 1159
BmiI	GGNNCC	7 cut(s)	441, 527, 743, 744, 966, 1297, 1484
BmrFI	CCNGG	4 cut(s)	516, 747, 1122, 1293
BmsI	GCATC	9 cut(s)	243, 379, 385, 599, 777, 1018, 1146, 1226, 1553
BpiI	GAAGAC	2 cut(s)	125, 1502
BpmI	CTGGAG	2 cut(s)	815, 916
Bpu10I	CCTNAGC	1 cut(s)	429
Bpu14I	TTCGAA	2 cut(s)	265, 993
BpuMI	CCSGG	1 cut(s)	516
BsaAI	YACGTR	1 cut(s)	324
BsaHI	GRCGYC	2 cut(s)	69, 1388
BsaJI	CCNNGG	5 cut(s)	731, 745, 1121, 1291, 1984
BsaWI	WCCGGW	1 cut(s)	131
BsaXI	ACNNNNNCTCC	4 cut(s)	666, 696, 1933, 1963
Bsc4I	CCNNNNNNNGG	7 cut(s)	169, 514, 737, 763, 974, 1019, 1442
Bse1I	ACTGG	5 cut(s)	1207, 1456, 1522, 1694, 1981
BseBI	CCWGG	3 cut(s)	747, 1122, 1293
BseDI	CCNNGG	5 cut(s)	731, 745, 1121, 1291, 1984
BseGI	GGATG	5 cut(s)	590, 792, 1009, 1217, 1702
BseLI	CCNNNNNNNGG	7 cut(s)	169, 514, 737, 763, 974, 1019, 1442
BseMII	CTCAG	2 cut(s)	443, 567
BseNI	ACTGG	5 cut(s)	1207, 1456, 1522, 1694, 1981
BseRI	GAGGAG	2 cut(s)	29, 248
BseSI	GKGCMC	1 cut(s)	746
BseXI	GCAGC	5 cut(s)	311, 777, 880, 1294, 1337
BsgI	GTGCAG	2 cut(s)	130, 1344
Bsh1236I	CGCG	1 cut(s)	188
BshFI	GGCC	6 cut(s)	173, 427, 744, 1160, 1932, 1989
BshNI	GGYRCC	2 cut(s)	439, 964
BsiHKAI	GWGCWC	1 cut(s)	1727
BsiSI	CCGG	2 cut(s)	132, 515
BslFI	GGGAC	3 cut(s)	161, 1036, 1680
BslI	CCNNNNNNNGG	7 cut(s)	169, 514, 737, 763, 974, 1019, 1442
BsmFI	GGGAC	3 cut(s)	161, 1036, 1680
BsnI	GGCC	6 cut(s)	173, 427, 744, 1160, 1932, 1989
Bsp119I	TTCGAA	2 cut(s)	265, 993
Bsp120I	GGGCCC	1 cut(s)	742
Bsp1286I	GDGCHC	2 cut(s)	746, 1727
Bsp143I	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
Bsp19I	CCATGG	1 cut(s)	1984
BspACI	CCGC	6 cut(s)	183, 215, 297, 433, 436, 508
BspANI	GGCC	6 cut(s)	173, 427, 744, 1160, 1932, 1989
BspCNI	CTCAG	2 cut(s)	442, 566
BspFNI	CGCG	1 cut(s)	188
BspLI	GGNNCC	7 cut(s)	441, 527, 743, 744, 966, 1297, 1484
BspPI	GGATC	3 cut(s)	79, 1417, 1670
BspQI	GCTCTTC	1 cut(s)	889
BspT104I	TTCGAA	2 cut(s)	265, 993
BspT107I	GGYRCC	2 cut(s)	439, 964
BspTI	CTTAAG	2 cut(s)	1132, 1334
BsrI	ACTGG	5 cut(s)	1207, 1456, 1522, 1694, 1981
BssECI	CCNNGG	5 cut(s)	731, 745, 1121, 1291, 1984
BssMI	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
BssNI	GRCGYC	2 cut(s)	69, 1388
BssT1I	CCWWGG	2 cut(s)	731, 1984
Bst2UI	CCWGG	3 cut(s)	747, 1122, 1293
Bst4CI	ACNGT	6 cut(s)	286, 947, 1396, 1575, 1633, 1647
Bst6I	CTCTTC	3 cut(s)	47, 348, 889
BstACI	GRCGYC	2 cut(s)	69, 1388
BstAFI	CTTAAG	2 cut(s)	1132, 1334
BstAPI	GCANNNNNTGC	1 cut(s)	305
BstBAI	YACGTR	1 cut(s)	324
BstBI	TTCGAA	2 cut(s)	265, 993
BstC8I	GCNNGC	2 cut(s)	660, 905
BstDEI	CTNAG	4 cut(s)	150, 429, 553, 1082
BstDSI	CCRYGG	1 cut(s)	1984
BstF5I	GGATG	5 cut(s)	590, 792, 1009, 1217, 1702
BstFNI	CGCG	1 cut(s)	188
BstH2I	RGCGCY	1 cut(s)	553
BstHHI	GCGC	2 cut(s)	188, 552
BstKTI	GATC	9 cut(s)	87, 159, 1277, 1303, 1412, 1678, 1702, 1759, 1852
BstMBI	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
BstMWI	GCNNNNNNNGC	3 cut(s)	305, 433, 1648
BstNI	CCWGG	3 cut(s)	747, 1122, 1293
BstNSI	RCATGY	1 cut(s)	1769
BstSCI	CCNGG	4 cut(s)	514, 745, 1120, 1291
BstSLI	GKGCMC	1 cut(s)	746
BstSNI	TACGTA	1 cut(s)	324
BstUI	CGCG	1 cut(s)	188
BstV1I	GCAGC	5 cut(s)	311, 777, 880, 1294, 1337
BstV2I	GAAGAC	2 cut(s)	125, 1502
BstX2I	RGATCY	1 cut(s)	84
BstXI	CCANNNNNNTGG	3 cut(s)	24, 536, 1814
BstYI	RGATCY	1 cut(s)	84
BsuI	GTATCC	2 cut(s)	680, 1973
BsuRI	GGCC	6 cut(s)	173, 427, 744, 1160, 1932, 1989
BtgI	CCRYGG	1 cut(s)	1984
BtsCI	GGATG	5 cut(s)	590, 792, 1009, 1217, 1702
BtsIMutI	CAGTG	3 cut(s)	561, 1515, 1974
Cac8I	GCNNGC	2 cut(s)	660, 905
CfoI	GCGC	2 cut(s)	188, 552
Cfr13I	GGNCC	5 cut(s)	425, 617, 742, 743, 1159
Csp6I	GTAC	5 cut(s)	313, 325, 358, 440, 1576
CviQI	GTAC	5 cut(s)	313, 325, 358, 440, 1576
DdeI	CTNAG	4 cut(s)	150, 429, 553, 1082
DpnI	GATC	9 cut(s)	86, 158, 1276, 1302, 1411, 1677, 1701, 1758, 1851
DpnII	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
EaeI	YGGCCR	3 cut(s)	171, 1930, 1987
Eam1104I	CTCTTC	3 cut(s)	47, 348, 889
EarI	CTCTTC	3 cut(s)	47, 348, 889
Eco105I	TACGTA	1 cut(s)	324
Eco130I	CCWWGG	2 cut(s)	731, 1984
Eco24I	GRGCYC	1 cut(s)	746
Eco47I	GGWCC	1 cut(s)	617
Eco47III	AGCGCT	1 cut(s)	551
Eco57I	CTGAAG	2 cut(s)	372, 1543
EcoO109I	RGGNCCY	3 cut(s)	617, 742, 1159
EcoRI	GAATTC	1 cut(s)	238
EcoRII	CCWGG	3 cut(s)	745, 1120, 1291
EcoT14I	CCWWGG	2 cut(s)	731, 1984
EcoT38I	GRGCYC	1 cut(s)	746
ErhI	CCWWGG	2 cut(s)	731, 1984
FaqI	GGGAC	3 cut(s)	161, 1036, 1680
FauI	CCCGC	1 cut(s)	429
FauNDI	CATATG	1 cut(s)	1580
FbaI	TGATCA	2 cut(s)	1699, 1849
FblI	GTMKAC	3 cut(s)	1107, 1391, 1609
Fnu4HI	GCNGC	7 cut(s)	184, 300, 434, 791, 869, 1308, 1326
FokI	GGATG	5 cut(s)	577, 799, 996, 1204, 1709
FriOI	GRGCYC	1 cut(s)	746
Fsp4HI	GCNGC	7 cut(s)	184, 300, 434, 791, 869, 1308, 1326
FspBI	CTAG	3 cut(s)	180, 732, 1737
GlaI	GCGC	2 cut(s)	187, 551
GluI	GCNGC	7 cut(s)	184, 300, 434, 791, 869, 1308, 1326
GsuI	CTGGAG	2 cut(s)	815, 916
HaeII	RGCGCY	1 cut(s)	553
HaeIII	GGCC	6 cut(s)	173, 427, 744, 1160, 1932, 1989
HapII	CCGG	2 cut(s)	132, 515
HhaI	GCGC	2 cut(s)	188, 552
Hin1I	GRCGYC	2 cut(s)	69, 1388
Hin6I	GCGC	2 cut(s)	186, 550
HinP1I	GCGC	2 cut(s)	186, 550
HincII	GTYRAC	3 cut(s)	752, 847, 1269
HindII	GTYRAC	3 cut(s)	752, 847, 1269
HindIII	AAGCTT	1 cut(s)	1474
HinfI	GANTC	9 cut(s)	78, 128, 1024, 1044, 1117, 1340, 1463, 1548, 1594
HpaI	GTTAAC	1 cut(s)	1269
HpaII	CCGG	2 cut(s)	132, 515
HphI	GGTGA	7 cut(s)	25, 146, 209, 530, 879, 1217, 1892
Hpy166II	GTNNAC	7 cut(s)	137, 752, 847, 1108, 1269, 1392, 1610
Hpy188I	TCNGA	6 cut(s)	127, 604, 1562, 1599, 1662, 1722
Hpy188III	TCNNGA	7 cut(s)	82, 280, 332, 620, 895, 1072, 1545
Hpy8I	GTNNAC	7 cut(s)	137, 752, 847, 1108, 1269, 1392, 1610
Hpy99I	CGWCG	3 cut(s)	71, 74, 1390
HpyAV	CCTTC	8 cut(s)	453, 711, 762, 977, 981, 1657, 1907, 1932
HpyCH4III	ACNGT	6 cut(s)	286, 947, 1396, 1575, 1633, 1647
HpyCH4IV	ACGT	4 cut(s)	69, 323, 1095, 1388
HpyF10VI	GCNNNNNNNGC	3 cut(s)	305, 433, 1648
HpyF3I	CTNAG	4 cut(s)	150, 429, 553, 1082
HpySE526I	ACGT	4 cut(s)	69, 323, 1095, 1388
Hsp92I	GRCGYC	2 cut(s)	69, 1388
HspAI	GCGC	2 cut(s)	186, 550
KpnI	GGTACC	1 cut(s)	443
Ksp22I	TGATCA	2 cut(s)	1699, 1849
KspAI	GTTAAC	1 cut(s)	1269
Kzo9I	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
LguI	GCTCTTC	1 cut(s)	889
LmnI	GCTCC	1 cut(s)	1234
Lsp1109I	GCAGC	5 cut(s)	311, 777, 880, 1294, 1337
LweI	GCATC	9 cut(s)	243, 379, 385, 599, 777, 1018, 1146, 1226, 1553
MaeI	CTAG	3 cut(s)	180, 732, 1737
MaeII	ACGT	4 cut(s)	69, 323, 1095, 1388
MaeIII	GTNAC	1 cut(s)	1045
MalI	GATC	9 cut(s)	86, 158, 1276, 1302, 1411, 1677, 1701, 1758, 1851
MboI	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
MboII	GAAGA	9 cut(s)	34, 49, 130, 166, 262, 365, 876, 1507, 1947
MflI	RGATCY	1 cut(s)	84
MhlI	GDGCHC	2 cut(s)	746, 1727
MlsI	TGGCCA	1 cut(s)	1989
MluNI	TGGCCA	1 cut(s)	1989
MlyI	GAGTC	6 cut(s)	87, 1018, 1053, 1126, 1334, 1457
MmeI	TCCRAC	4 cut(s)	55, 664, 1571, 1652
Mox20I	TGGCCA	1 cut(s)	1989
MroXI	GAANNNNTTC	1 cut(s)	1942
MscI	TGGCCA	1 cut(s)	1989
MslI	CAYNNNNRTG	7 cut(s)	1014, 1583, 1764, 1770, 1791, 1812, 1926
Msp20I	TGGCCA	1 cut(s)	1989
MspA1I	CMGCKG	1 cut(s)	433
MspCI	CTTAAG	2 cut(s)	1132, 1334
MspI	CCGG	2 cut(s)	132, 515
MspR9I	CCNGG	4 cut(s)	516, 747, 1122, 1293
MvaI	CCWGG	3 cut(s)	747, 1122, 1293
MvnI	CGCG	1 cut(s)	188
MwoI	GCNNNNNNNGC	3 cut(s)	305, 433, 1648
NciI	CCSGG	1 cut(s)	516
NcoI	CCATGG	1 cut(s)	1984
NdeI	CATATG	1 cut(s)	1580
NdeII	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
NlaIV	GGNNCC	7 cut(s)	441, 527, 743, 744, 966, 1297, 1484
NmeAIII	GCCGAG	1 cut(s)	1393
NmuCI	GTSAC	1 cut(s)	1045
NspI	RCATGY	1 cut(s)	1769
NspV	TTCGAA	2 cut(s)	265, 993
PciI	ACATGT	1 cut(s)	1765
PciSI	GCTCTTC	1 cut(s)	889
PdmI	GAANNNNTTC	1 cut(s)	1942
PfeI	GAWTC	3 cut(s)	128, 1548, 1594
PflMI	CCANNNNNTGG	2 cut(s)	763, 974
PkrI	GCNGC	7 cut(s)	185, 301, 435, 792, 870, 1309, 1327
PleI	GAGTC	6 cut(s)	86, 1018, 1052, 1125, 1334, 1457
PpsI	GAGTC	6 cut(s)	86, 1018, 1052, 1125, 1334, 1457
Ppu21I	YACGTR	1 cut(s)	324
PpuMI	RGGWCCY	1 cut(s)	617
PscI	ACATGT	1 cut(s)	1765
PshBI	ATTAAT	2 cut(s)	1059, 1749
PsiI	TTATAA	1 cut(s)	1350
Psp5II	RGGWCCY	1 cut(s)	617
Psp6I	CCWGG	3 cut(s)	745, 1120, 1291
PspGI	CCWGG	3 cut(s)	745, 1120, 1291
PspN4I	GGNNCC	7 cut(s)	441, 527, 743, 744, 966, 1297, 1484
PspOMI	GGGCCC	1 cut(s)	742
PspPI	GGNCC	5 cut(s)	425, 617, 742, 743, 1159
PspPPI	RGGWCCY	1 cut(s)	617
PsrI	GAACNNNNNNTAC	2 cut(s)	1964, 1996
PsuI	RGATCY	1 cut(s)	84
RsaI	GTAC	5 cut(s)	314, 326, 359, 441, 1577
RsaNI	GTAC	5 cut(s)	313, 325, 358, 440, 1576
RseI	CAYNNNNRTG	7 cut(s)	1014, 1583, 1764, 1770, 1791, 1812, 1926
SapI	GCTCTTC	1 cut(s)	889
SatI	GCNGC	7 cut(s)	184, 300, 434, 791, 869, 1308, 1326
Sau3AI	GATC	9 cut(s)	84, 156, 1274, 1300, 1409, 1675, 1699, 1756, 1849
Sau96I	GGNCC	5 cut(s)	425, 617, 742, 743, 1159
ScaI	AGTACT	1 cut(s)	359
SchI	GAGTC	6 cut(s)	87, 1018, 1053, 1126, 1334, 1457
ScrFI	CCNGG	4 cut(s)	516, 747, 1122, 1293
SduI	GDGCHC	2 cut(s)	746, 1727
SfaNI	GCATC	9 cut(s)	243, 379, 385, 599, 777, 1018, 1146, 1226, 1553
SfuI	TTCGAA	2 cut(s)	265, 993
SinI	GGWCC	1 cut(s)	617
SmiMI	CAYNNNNRTG	7 cut(s)	1014, 1583, 1764, 1770, 1791, 1812, 1926
SmlI	CTYRAG	2 cut(s)	1132, 1334
SmoI	CTYRAG	2 cut(s)	1132, 1334
SnaBI	TACGTA	1 cut(s)	324
SsiI	CCGC	6 cut(s)	183, 215, 297, 433, 436, 508
SspI	AATATT	1 cut(s)	697
SspMI	CTAG	3 cut(s)	180, 732, 1737
StyD4I	CCNGG	4 cut(s)	514, 745, 1120, 1291
StyI	CCWWGG	2 cut(s)	731, 1984
TaaI	ACNGT	6 cut(s)	286, 947, 1396, 1575, 1633, 1647
TaiI	ACGT	4 cut(s)	72, 326, 1098, 1391
TatI	WGTACW	2 cut(s)	357, 1575
TauI	GCSGC	2 cut(s)	186, 436
TfiI	GAWTC	3 cut(s)	128, 1548, 1594
TscAI	CASTG	3 cut(s)	561, 1522, 1981
TseFI	GTSAC	1 cut(s)	1045
TseI	GCWGC	5 cut(s)	299, 790, 868, 1307, 1325
Tsp45I	GTSAC	1 cut(s)	1045
TspGWI	ACGGA	1 cut(s)	1247
TspRI	CASTG	3 cut(s)	561, 1522, 1981
Van91I	CCANNNNNTGG	2 cut(s)	763, 974
Vha464I	CTTAAG	2 cut(s)	1132, 1334
VpaK11BI	GGWCC	1 cut(s)	617
VspI	ATTAAT	2 cut(s)	1059, 1749
XapI	RAATTY	3 cut(s)	238, 760, 922
XceI	RCATGY	1 cut(s)	1769
XcmI	CCANNNNNNNNNTGG	3 cut(s)	21, 1118, 1784
XmaJI	CCTAGG	1 cut(s)	731
XmiI	GTMKAC	3 cut(s)	1107, 1391, 1609
XmnI	GAANNNNTTC	1 cut(s)	1942
XspI	CTAG	3 cut(s)	180, 732, 1737
ZraI	GACGTC	2 cut(s)	70, 1389
ZrmI	AGTACT	1 cut(s)	359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.