Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

8824435 .. 8830841

Gene Structure 6407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1066100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGTTAATCCAAGCGCTGAAATTAGGGCACGCAACCCTCCTCTTCCTCATCCTCATCTGCGTCGTCCATTTTCTCACTCCCAACGAAGTCGTTGCGGTTCAGATTCTTTCGAAATCGAAGCTCGAGAAGTGCGAGAAGAACTCCGATTCCGGAAACTTGAACTGCACCAAAAAGATCGTCCTCAACATGGCCGTTCCTAGCGGCGCGAGTGGCGGAGAGGCATCGATTGTTGCGGAAATTGTGGAGGTGGAGGAGAATTCGAGTCAGAAGATGCAGACTTTCCGGATACCGCCTGTTCTGACCGTGAACAAATCCGCAGCTTATGCCTTGTACGAGCTTACTTACATACGGGATGTCGCCTATAAACCCGAAGAGTATTATGTTAAAACCCGCAAATGCGAGCCAGATGCAGATGCCAAAGTTGTGAAGATATGTGAGAGGTTACGAGATGAAAATGGTCACGTTGTTGAACACACTGAGCCAATATGCTGTCCTTGTGGGCCTCAGCGGAGGGTACCTTCATCATGCGGAAATGTTTTTGACAAGTTGATGAAGGGAAAAGCCAATACTGCCCACTGTGTTCGTTTCCCGGGTGACTGGTTCCATGTTTTTGCTATTGGACAGCGGTCAGTGGGATTCAGTGTTCTAATTCAGGTGAAGACAGGTTCTACAGTTTCGGAAGTGCTTGTTGGTCCTGAAAACAGAACAGCCATATCCAATGATAATTTTTTACGAGTTAATCTTATTGGAGATTTTGTTGGTTACACAAATATACCATCATTGGAGGATTTCTACCTCGTAATCCCAAGGCAGCCGGGCCCTGGTCAACCCCAGGATATGGGGAGGAACTTTTCTATGTGGATGCTGCTGGAAAGAGTTCGATTTTCTTTAGACGGTCTTGAATGTAACAAAATTGGTGTCAGTTATGAGGCTTTCAATGGGCAACCAAACTTTTGCTCTTCGCCGCTCTGGAGTTGCTTGCATAATCAAATATGGAATTACTGGGTGGCTGACCAGAACCGAATTCAAAGGAATCAGTTGCCACTTTATGGGGTGGAAGGAAGGTTTGAAAGGATAAACCAGCATCCAAATGCCGGGACTCATTCGTTCTCCATAGGGGTCGCTGAAGTTATTAATACAAATCTCTTGATAGAATTAAGTGCTGACGATGTAGAATATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATGTCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCGACAATAACAACCACCAATACTGGTGAAGTGGAAGCATCGTATAGCTTAACGTTTGATTGCTCTAGTGATGTCACCCTTATGGAGGAACAATTCTTTATTATGAAGCCAAAAGAAATGTCAACCCGATCCTTCAAACTTTACCCACCAACTGATCAAGCTGCAAAGTACGTATGCGCGGCTATTCTAAAGGACTCAGAATATAAAGAAGTAGATAGAGCCGAGTGCCAATTTTCTACAACATCTACCGTCCTGGATAATGGAACACAGGGTAATCCCTTTAGTCCACCAAAGAATGGCACAGCCAGTTTTTTTGAATCTATGGAAAGTATTTGGAACAATCTGTGGAATGTTTTGGTGGATTTCGTCACTGGAAAAACTTGCAGAAGGAAATGCTCTGGATTCTTTGACTTCAGGTGCCACATACAGTACATATGCATGAGTTGGATTGTGATGTTTGGTCTACTCTTGGCAATTTTTCCAACAGTGCTTGTGCTTCTGTGGCTTCTACACCAAAAGGGACTTTTTGATCCTATATACGATTGGTGGGAAGATCATTTTTGGGATGGTACTCAGCACAGCAAGGATTATCGAAAGCACAGAGTTGACGTAGACCGCCCACGCACTCATGCTCTTAAACATCATGGGCATGAAGCGACGCACCACAAGCATGGTTCTCACTATAAGCGAAGAAACATTCACCACGATTACAGGCATAACCAGCTTGAAAGAGGCAGCGATTATTACTACCATCTTCACCATGTCCATAAAGACAAGCACAAGCATGGGCGAAGAAAGCATTCAAGTGTTATGCAGATAGTTGACGAAAGTCATGGATACCATGCCAAGAAAAAAGGAAGGAGAGCAACAAAAGACTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

712

Amino Acids

81.17

Weight (kDa)

7.99

Isoelectric Point (pI)

35.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	52 - 565	1.1e-167	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	514
AccB1I	GGYRCC	2 cut(s)	514, 1671
AccB7I	CCANNNNNTGG	3 cut(s)	838, 1049, 1550
AccBSI	CCGCTC	1 cut(s)	967
AccI	GTMKAC	3 cut(s)	1182, 1717, 1866
AccII	CGCG	2 cut(s)	206, 1433
AccIII	TCCGGA	2 cut(s)	149, 282
AclI	AACGTT	1 cut(s)	1307
AclWI	GGATC	2 cut(s)	1377, 1778
AcoI	YGGCCR	1 cut(s)	189
AcsI	RAATTY	2 cut(s)	256, 1023
AcuI	CTGAAG	2 cut(s)	1146, 1651
AfaI	GTAC	5 cut(s)	332, 516, 1424, 1685, 1825
AfeI	AGCGCT	1 cut(s)	15
AfiI	CCNNNNNNNGG	7 cut(s)	187, 821, 838, 1049, 1094, 1339, 1550
AflII	CTTAAG	1 cut(s)	1207
AjnI	CCWGG	4 cut(s)	820, 831, 1195, 1506
AjuI	GAANNNNNNNTTGG	2 cut(s)	672, 704
AloI	GAACNNNNNNTCC	4 cut(s)	131, 163, 627, 659
AluBI	AGCT	6 cut(s)	121, 320, 337, 1302, 1415, 1978
AluI	AGCT	6 cut(s)	121, 320, 337, 1302, 1415, 1978
AlwI	GGATC	2 cut(s)	1377, 1778
Ama87I	CYCGRG	2 cut(s)	122, 589
Aor13HI	TCCGGA	2 cut(s)	149, 282
Aor51HI	AGCGCT	1 cut(s)	15
AoxI	GGCC	4 cut(s)	189, 500, 817, 1233
ApaI	GGGCCC	1 cut(s)	821
ApeKI	GCWGC	5 cut(s)	317, 811, 865, 1415, 1989
ApoI	RAATTY	2 cut(s)	256, 1023
ArsI	GACNNNNNNTTYG	6 cut(s)	162, 194, 903, 935, 1358, 1390
AseI	ATTAAT	1 cut(s)	1134
Asp700I	GAANNNNTTC	2 cut(s)	876, 2053
Asp718I	GGTACC	1 cut(s)	514
AspLEI	GCGC	3 cut(s)	16, 206, 1433
AspS9I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
AsuC2I	CCSGG	4 cut(s)	590, 591, 816, 1096
AsuHPI	GGTGA	6 cut(s)	605, 667, 1292, 1321, 1946, 2003
AsuII	TTCGAA	1 cut(s)	110
AvaI	CYCGRG	2 cut(s)	122, 589
AvaII	GGWCC	1 cut(s)	692
BaeGI	GKGCMC	2 cut(s)	30, 821
BanI	GGYRCC	2 cut(s)	514, 1671
BanII	GRGCYC	1 cut(s)	821
BbsI	GAAGAC	1 cut(s)	665
BbvCI	CCTCAGC	1 cut(s)	504
BbvI	GCAGC	5 cut(s)	329, 823, 852, 1402, 2001
BccI	CCATC	3 cut(s)	784, 1814, 2013
BceAI	ACGGC	1 cut(s)	176
BcgI	CGANNNNNNTGC	4 cut(s)	74, 108, 389, 423
BciT130I	CCWGG	4 cut(s)	822, 833, 1197, 1508
BciVI	GTATCC	2 cut(s)	279, 2084
BclI	TGATCA	1 cut(s)	1408
BcnI	CCSGG	4 cut(s)	590, 591, 816, 1096
BfaI	CTAG	2 cut(s)	198, 1320
BfmI	CTRYAG	1 cut(s)	669
BfoI	RGCGCY	1 cut(s)	17
BfrI	CTTAAG	1 cut(s)	1207
BfuI	GTATCC	2 cut(s)	279, 2084
BisI	GCNGC	8 cut(s)	202, 318, 812, 866, 965, 1416, 1434, 1990
BlsI	GCNGC	8 cut(s)	203, 319, 813, 867, 966, 1417, 1435, 1991
Bme1390I	CCNGG	8 cut(s)	590, 591, 816, 822, 833, 1096, 1197, 1508
Bme18I	GGWCC	1 cut(s)	692
BmeT110I	CYCGRG	2 cut(s)	122, 589
BmgT120I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
BmiI	GGNNCC	4 cut(s)	516, 602, 819, 1673
BmrFI	CCNGG	8 cut(s)	590, 591, 816, 822, 833, 1096, 1197, 1508
BmrI	ACTGGG	1 cut(s)	1012
BmsI	GCATC	8 cut(s)	230, 261, 397, 403, 852, 1093, 1221, 1301
BmuI	ACTGGG	1 cut(s)	1012
BoxI	GACNNNNGTC	2 cut(s)	625, 2082
BpiI	GAAGAC	1 cut(s)	665
BplI	GAGNNNNNCTC	2 cut(s)	125, 157
BpmI	CTGGAG	1 cut(s)	991
Bpu10I	CCTNAGC	1 cut(s)	504
Bpu14I	TTCGAA	1 cut(s)	110
BpuMI	CCSGG	4 cut(s)	590, 591, 816, 1096
Bsa29I	ATCGAT	1 cut(s)	224
BsaAI	YACGTR	1 cut(s)	1426
BsaJI	CCNNGG	5 cut(s)	589, 806, 820, 831, 1196
BsaWI	WCCGGW	2 cut(s)	149, 282
BsaXI	ACNNNNNCTCC	2 cut(s)	741, 771
Bsc4I	CCNNNNNNNGG	7 cut(s)	187, 821, 838, 1049, 1094, 1339, 1550
Bse1I	ACTGG	5 cut(s)	602, 1007, 1282, 1560, 1630
BseAI	TCCGGA	2 cut(s)	149, 282
BseBI	CCWGG	4 cut(s)	822, 833, 1197, 1508
BseCI	ATCGAT	1 cut(s)	224
BseDI	CCNNGG	5 cut(s)	589, 806, 820, 831, 1196
BseGI	GGATG	5 cut(s)	48, 358, 867, 1084, 1825
BseLI	CCNNNNNNNGG	7 cut(s)	187, 821, 838, 1049, 1094, 1339, 1550
BseMII	CTCAG	4 cut(s)	468, 518, 1464, 1841
BseNI	ACTGG	5 cut(s)	602, 1007, 1282, 1560, 1630
BseRI	GAGGAG	2 cut(s)	29, 266
BseSI	GKGCMC	2 cut(s)	30, 821
BseXI	GCAGC	5 cut(s)	329, 823, 852, 1402, 2001
BsgI	GTGCAG	1 cut(s)	148
Bsh1236I	CGCG	2 cut(s)	206, 1433
BshFI	GGCC	4 cut(s)	191, 502, 819, 1235
BshNI	GGYRCC	2 cut(s)	514, 1671
BshVI	ATCGAT	1 cut(s)	224
BsiHKCI	CYCGRG	2 cut(s)	122, 589
BsiSI	CCGG	5 cut(s)	150, 283, 590, 815, 1095
BslFI	GGGAC	3 cut(s)	1111, 1205, 1788
BslI	CCNNNNNNNGG	7 cut(s)	187, 821, 838, 1049, 1094, 1339, 1550
BsmFI	GGGAC	3 cut(s)	1111, 1205, 1788
BsmI	GAATGC	1 cut(s)	2053
BsnI	GGCC	4 cut(s)	191, 502, 819, 1235
BsoBI	CYCGRG	2 cut(s)	122, 589
Bsp119I	TTCGAA	1 cut(s)	110
Bsp120I	GGGCCC	1 cut(s)	817
Bsp1286I	GDGCHC	2 cut(s)	30, 821
Bsp13I	TCCGGA	2 cut(s)	149, 282
Bsp143I	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
BspANI	GGCC	4 cut(s)	191, 502, 819, 1235
BspCNI	CTCAG	4 cut(s)	469, 517, 1463, 1840
BspDI	ATCGAT	1 cut(s)	224
BspEI	TCCGGA	2 cut(s)	149, 282
BspFNI	CGCG	2 cut(s)	206, 1433
BspLI	GGNNCC	4 cut(s)	516, 602, 819, 1673
BspPI	GGATC	2 cut(s)	1377, 1778
BspQI	GCTCTTC	1 cut(s)	964
BspT104I	TTCGAA	1 cut(s)	110
BspT107I	GGYRCC	2 cut(s)	514, 1671
BspTI	CTTAAG	1 cut(s)	1207
BsrBI	CCGCTC	1 cut(s)	967
BsrI	ACTGG	5 cut(s)	602, 1007, 1282, 1560, 1630
BssECI	CCNNGG	5 cut(s)	589, 806, 820, 831, 1196
BssMI	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
BssT1I	CCWWGG	1 cut(s)	806
Bst2UI	CCWGG	4 cut(s)	822, 833, 1197, 1508
Bst4CI	ACNGT	7 cut(s)	304, 578, 673, 896, 1504, 1683, 1741
Bst6I	CTCTTC	3 cut(s)	47, 366, 964
BstAFI	CTTAAG	1 cut(s)	1207
BstAPI	GCANNNNNTGC	1 cut(s)	323
BstBAI	YACGTR	1 cut(s)	1426
BstBI	TTCGAA	1 cut(s)	110
BstC8I	GCNNGC	3 cut(s)	30, 401, 980
BstDEI	CTNAG	4 cut(s)	477, 504, 1450, 1827
BstENI	CCTNNNNNAGG	1 cut(s)	1337
BstF5I	GGATG	5 cut(s)	48, 358, 867, 1084, 1825
BstFNI	CGCG	2 cut(s)	206, 1433
BstH2I	RGCGCY	1 cut(s)	17
BstHHI	GCGC	3 cut(s)	16, 206, 1433
BstKTI	GATC	5 cut(s)	177, 1385, 1411, 1786, 1810
BstMBI	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
BstMWI	GCNNNNNNNGC	7 cut(s)	210, 323, 569, 1756, 1921, 1975, 2050
BstNI	CCWGG	4 cut(s)	822, 833, 1197, 1508
BstPAI	GACNNNNGTC	2 cut(s)	625, 2082
BstSCI	CCNGG	8 cut(s)	588, 589, 814, 820, 831, 1094, 1195, 1506
BstSFI	CTRYAG	1 cut(s)	669
BstSLI	GKGCMC	2 cut(s)	30, 821
BstSNI	TACGTA	1 cut(s)	1426
BstUI	CGCG	2 cut(s)	206, 1433
BstV1I	GCAGC	5 cut(s)	329, 823, 852, 1402, 2001
BstV2I	GAAGAC	1 cut(s)	665
BstXI	CCANNNNNNTGG	1 cut(s)	1925
Bsu15I	ATCGAT	1 cut(s)	224
BsuI	GTATCC	2 cut(s)	279, 2084
BsuRI	GGCC	4 cut(s)	191, 502, 819, 1235
BsuTUI	ATCGAT	1 cut(s)	224
BtsCI	GGATG	5 cut(s)	48, 358, 867, 1084, 1825
BtsIMutI	CAGTG	6 cut(s)	474, 574, 636, 646, 1623, 1746
Cac8I	GCNNGC	3 cut(s)	30, 401, 980
CfoI	GCGC	3 cut(s)	16, 206, 1433
Cfr13I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
Cfr9I	CCCGGG	1 cut(s)	589
ClaI	ATCGAT	1 cut(s)	224
CseI	GACGC	2 cut(s)	49, 1921
Csp6I	GTAC	5 cut(s)	331, 515, 1423, 1684, 1824
CviQI	GTAC	5 cut(s)	331, 515, 1423, 1684, 1824
DdeI	CTNAG	4 cut(s)	477, 504, 1450, 1827
DpnI	GATC	5 cut(s)	176, 1384, 1410, 1785, 1809
DpnII	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
EaeI	YGGCCR	1 cut(s)	189
Eam1104I	CTCTTC	3 cut(s)	47, 366, 964
EarI	CTCTTC	3 cut(s)	47, 366, 964
EciI	GGCGGA	1 cut(s)	228
Eco105I	TACGTA	1 cut(s)	1426
Eco130I	CCWWGG	1 cut(s)	806
Eco24I	GRGCYC	1 cut(s)	821
Eco47I	GGWCC	1 cut(s)	692
Eco47III	AGCGCT	1 cut(s)	15
Eco57I	CTGAAG	2 cut(s)	1146, 1651
Eco88I	CYCGRG	2 cut(s)	122, 589
EcoNI	CCTNNNNNAGG	1 cut(s)	1337
EcoO109I	RGGNCCY	2 cut(s)	818, 1234
EcoRI	GAATTC	2 cut(s)	256, 1023
EcoRII	CCWGG	4 cut(s)	820, 831, 1195, 1506
EcoT14I	CCWWGG	1 cut(s)	806
EcoT22I	ATGCAT	1 cut(s)	1694
EcoT38I	GRGCYC	1 cut(s)	821
ErhI	CCWWGG	1 cut(s)	806
FaqI	GGGAC	3 cut(s)	1111, 1205, 1788
FauI	CCCGC	1 cut(s)	398
FauNDI	CATATG	1 cut(s)	1688
FbaI	TGATCA	1 cut(s)	1408
FblI	GTMKAC	3 cut(s)	1182, 1717, 1866
Fnu4HI	GCNGC	8 cut(s)	202, 318, 812, 866, 965, 1416, 1434, 1990
FokI	GGATG	5 cut(s)	35, 365, 874, 1071, 1832
FriOI	GRGCYC	1 cut(s)	821
Fsp4HI	GCNGC	8 cut(s)	202, 318, 812, 866, 965, 1416, 1434, 1990
FspBI	CTAG	2 cut(s)	198, 1320
GlaI	GCGC	3 cut(s)	15, 205, 1432
GluI	GCNGC	8 cut(s)	202, 318, 812, 866, 965, 1416, 1434, 1990
GsuI	CTGGAG	1 cut(s)	991
HaeII	RGCGCY	1 cut(s)	17
HaeIII	GGCC	4 cut(s)	191, 502, 819, 1235
HapII	CCGG	5 cut(s)	150, 283, 590, 815, 1095
HgaI	GACGC	2 cut(s)	49, 1921
HhaI	GCGC	3 cut(s)	16, 206, 1433
Hin6I	GCGC	3 cut(s)	14, 204, 1431
HinP1I	GCGC	3 cut(s)	14, 204, 1431
HincII	GTYRAC	4 cut(s)	827, 1377, 1861, 2077
HindII	GTYRAC	4 cut(s)	827, 1377, 1861, 2077
HpaII	CCGG	5 cut(s)	150, 283, 590, 815, 1095
HphI	GGTGA	6 cut(s)	605, 667, 1292, 1321, 1946, 2003
Hpy166II	GTNNAC	9 cut(s)	307, 827, 1183, 1377, 1541, 1718, 1861, 1867, 2077
Hpy188I	TCNGA	6 cut(s)	102, 145, 267, 300, 679, 1453
Hpy188III	TCNNGA	8 cut(s)	124, 150, 283, 695, 899, 970, 1147, 1653
Hpy8I	GTNNAC	9 cut(s)	307, 827, 1183, 1377, 1541, 1718, 1861, 1867, 2077
Hpy99I	CGWCG	2 cut(s)	65, 1915
HpyAV	CCTTC	7 cut(s)	528, 547, 1052, 1056, 1396, 1635, 2106
HpyCH4III	ACNGT	7 cut(s)	304, 578, 673, 896, 1504, 1683, 1741
HpyCH4IV	ACGT	4 cut(s)	462, 1307, 1425, 1863
HpyCH4V	TGCA	8 cut(s)	165, 274, 410, 982, 1418, 1638, 1692, 2068
HpyF10VI	GCNNNNNNNGC	7 cut(s)	210, 323, 569, 1756, 1921, 1975, 2050
HpyF3I	CTNAG	4 cut(s)	477, 504, 1450, 1827
HpySE526I	ACGT	4 cut(s)	462, 1307, 1425, 1863
HspAI	GCGC	3 cut(s)	14, 204, 1431
Kpn2I	TCCGGA	2 cut(s)	149, 282
KpnI	GGTACC	1 cut(s)	518
Ksp22I	TGATCA	1 cut(s)	1408
Kzo9I	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
LguI	GCTCTTC	1 cut(s)	964
Lsp1109I	GCAGC	5 cut(s)	329, 823, 852, 1402, 2001
LweI	GCATC	8 cut(s)	230, 261, 397, 403, 852, 1093, 1221, 1301
MaeI	CTAG	2 cut(s)	198, 1320
MaeII	ACGT	4 cut(s)	462, 1307, 1425, 1863
MaeIII	GTNAC	7 cut(s)	441, 458, 593, 761, 905, 1327, 1621
MalI	GATC	5 cut(s)	176, 1384, 1410, 1785, 1809
MbiI	CCGCTC	1 cut(s)	967
MboI	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
MhlI	GDGCHC	2 cut(s)	30, 821
MlyI	GAGTC	4 cut(s)	271, 1093, 1201, 1442
MmeI	TCCRAC	2 cut(s)	1679, 1760
Mph1103I	ATGCAT	1 cut(s)	1694
MroI	TCCGGA	2 cut(s)	149, 282
MroXI	GAANNNNTTC	2 cut(s)	876, 2053
MseI	TTAA	8 cut(s)	5, 384, 738, 1134, 1157, 1208, 1304, 1890
MslI	CAYNNNNRTG	7 cut(s)	1089, 1334, 1691, 1902, 1923, 2037, 2058
MspA1I	CMGCKG	2 cut(s)	508, 625
MspCI	CTTAAG	1 cut(s)	1207
MspI	CCGG	5 cut(s)	150, 283, 590, 815, 1095
MspR9I	CCNGG	8 cut(s)	590, 591, 816, 822, 833, 1096, 1197, 1508
Mva1269I	GAATGC	1 cut(s)	2053
MvaI	CCWGG	4 cut(s)	822, 833, 1197, 1508
MvnI	CGCG	2 cut(s)	206, 1433
MwoI	GCNNNNNNNGC	7 cut(s)	210, 323, 569, 1756, 1921, 1975, 2050
NciI	CCSGG	4 cut(s)	590, 591, 816, 1096
NdeI	CATATG	1 cut(s)	1688
NdeII	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
NlaIV	GGNNCC	4 cut(s)	516, 602, 819, 1673
NmeAIII	GCCGAG	1 cut(s)	1501
NmuCI	GTSAC	4 cut(s)	458, 593, 1327, 1621
NsiI	ATGCAT	1 cut(s)	1694
NspV	TTCGAA	1 cut(s)	110
PaeR7I	CTCGAG	1 cut(s)	122
PciSI	GCTCTTC	1 cut(s)	964
PcsI	WCGNNNNNNNCGW	1 cut(s)	129
PctI	GAATGC	1 cut(s)	2053
PdmI	GAANNNNTTC	2 cut(s)	876, 2053
PfeI	GAWTC	6 cut(s)	103, 146, 636, 1033, 1571, 1656
PflMI	CCANNNNNTGG	3 cut(s)	838, 1049, 1550
PfoI	TCCNGGA	1 cut(s)	1506
PkrI	GCNGC	8 cut(s)	203, 319, 813, 867, 966, 1417, 1435, 1991
PleI	GAGTC	4 cut(s)	270, 1093, 1200, 1442
PpsI	GAGTC	4 cut(s)	270, 1093, 1200, 1442
Ppu21I	YACGTR	1 cut(s)	1426
PshAI	GACNNNNGTC	2 cut(s)	625, 2082
PshBI	ATTAAT	1 cut(s)	1134
Psp1406I	AACGTT	1 cut(s)	1307
Psp6I	CCWGG	4 cut(s)	820, 831, 1195, 1506
PspGI	CCWGG	4 cut(s)	820, 831, 1195, 1506
PspN4I	GGNNCC	4 cut(s)	516, 602, 819, 1673
PspOMI	GGGCCC	1 cut(s)	817
PspPI	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
RsaI	GTAC	5 cut(s)	332, 516, 1424, 1685, 1825
RsaNI	GTAC	5 cut(s)	331, 515, 1423, 1684, 1824
RseI	CAYNNNNRTG	7 cut(s)	1089, 1334, 1691, 1902, 1923, 2037, 2058
SapI	GCTCTTC	1 cut(s)	964
SaqAI	TTAA	8 cut(s)	5, 384, 738, 1134, 1157, 1208, 1304, 1890
SatI	GCNGC	8 cut(s)	202, 318, 812, 866, 965, 1416, 1434, 1990
Sau3AI	GATC	5 cut(s)	174, 1382, 1408, 1783, 1807
Sau96I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
SchI	GAGTC	4 cut(s)	271, 1093, 1201, 1442
ScrFI	CCNGG	8 cut(s)	590, 591, 816, 822, 833, 1096, 1197, 1508
SduI	GDGCHC	2 cut(s)	30, 821
SfaNI	GCATC	8 cut(s)	230, 261, 397, 403, 852, 1093, 1221, 1301
SfcI	CTRYAG	1 cut(s)	669
Sfr274I	CTCGAG	1 cut(s)	122
SfuI	TTCGAA	1 cut(s)	110
SinI	GGWCC	1 cut(s)	692
SlaI	CTCGAG	1 cut(s)	122
SmaI	CCCGGG	1 cut(s)	591
SmiMI	CAYNNNNRTG	7 cut(s)	1089, 1334, 1691, 1902, 1923, 2037, 2058
SmlI	CTYRAG	2 cut(s)	122, 1207
SmoI	CTYRAG	2 cut(s)	122, 1207
SnaBI	TACGTA	1 cut(s)	1426
SspMI	CTAG	2 cut(s)	198, 1320
StyD4I	CCNGG	8 cut(s)	588, 589, 814, 820, 831, 1094, 1195, 1506
StyI	CCWWGG	1 cut(s)	806
TaaI	ACNGT	7 cut(s)	304, 578, 673, 896, 1504, 1683, 1741
TaiI	ACGT	4 cut(s)	465, 1310, 1428, 1866
TaqI	TCGA	7 cut(s)	110, 116, 123, 224, 260, 880, 1846
TatI	WGTACW	1 cut(s)	1683
TauI	GCSGC	3 cut(s)	204, 967, 1436
TfiI	GAWTC	6 cut(s)	103, 146, 636, 1033, 1571, 1656
Tru1I	TTAA	8 cut(s)	5, 384, 738, 1134, 1157, 1208, 1304, 1890
Tru9I	TTAA	8 cut(s)	5, 384, 738, 1134, 1157, 1208, 1304, 1890
TscAI	CASTG	6 cut(s)	481, 581, 636, 646, 1630, 1746
TseFI	GTSAC	4 cut(s)	458, 593, 1327, 1621
TseI	GCWGC	5 cut(s)	317, 811, 865, 1415, 1989
Tsp45I	GTSAC	4 cut(s)	458, 593, 1327, 1621
TspDTI	ATGAA	5 cut(s)	465, 510, 566, 1373, 1920
TspMI	CCCGGG	1 cut(s)	589
TspRI	CASTG	6 cut(s)	481, 581, 636, 646, 1630, 1746
Van91I	CCANNNNNTGG	3 cut(s)	838, 1049, 1550
Vha464I	CTTAAG	1 cut(s)	1207
VpaK11BI	GGWCC	1 cut(s)	692
VspI	ATTAAT	1 cut(s)	1134
XagI	CCTNNNNNAGG	1 cut(s)	1337
XapI	RAATTY	2 cut(s)	256, 1023
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1193
XhoI	CTCGAG	1 cut(s)	122
XmaI	CCCGGG	1 cut(s)	589
XmiI	GTMKAC	3 cut(s)	1182, 1717, 1866
XmnI	GAANNNNTTC	2 cut(s)	876, 2053
XspI	CTAG	2 cut(s)	198, 1320
Zsp2I	ATGCAT	1 cut(s)	1694

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.