Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Reverse (-)

53074360 .. 53080911

Gene Structure 6552 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003818

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGAGAATCCCTCCACCAAAATTGTGGCTCACCATCCTCCTCTTCCTCCCTTGTTTCATCAGCGACGTCGTCGGAGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTCCGATTCCGGTGACTTGAATTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGTGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAAATTGTAGAGGTAGAGGAGAATTCAAGCCAGAAGATGCAAACTTTTCGAATACCCCCTGTCCTGACTGTCAATAAATCCGCGGCTTATGCCTTGTACGAGCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACTCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTGAAGATATGTGAGAGGTTGCGAGATGAAAATGGTACCATTATTGAGCATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCATGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCCAATACAGCCCACTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGGATCTAAAGTTTCGGAAGTGCTTGTAGGTCCTGAAAACCGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGACTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTAGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAGTTATGGAATTTCTTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCATAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAGAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGCTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAACGTTATCTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGAGCCGAGTGCCAATTTTCTACGACATCTACTGTCATTGATAATGGATCACAGGGTAATCCCTTTAAGCCACCAAAGACAGGAACAACTGGTTTCTTTGACTCCATTGAAAGCTTTTGGAACCATGTGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTTGGTATACTTTTAGCAATTTTTCCAACAGTCATTGTGCTGCTGTGGCTCTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAGGATAGTCCGAGCCCCAAGAATACAAGACACAGAATTAATGTAGATCACCGACATGTTCATGTCCATAAACATCATGGGCATGAAGCAAGGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAGTGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGACTACCATTACGACCTTCACCATGTCCATAAGGATAAGCACAAGCATGGCCGAAGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCACGGCCAGAAAAAACAAAGGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

706

Amino Acids

80.51

Weight (kDa)

8.42

Isoelectric Point (pI)

42.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	46 - 558	7.2e-161	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1437
AatII	GACGTC	1 cut(s)	69
Acc65I	GGTACC	2 cut(s)	437, 493
AccB1I	GGYRCC	4 cut(s)	437, 493, 1018, 1890
AccB7I	CCANNNNNTGG	2 cut(s)	817, 1028
AccI	GTMKAC	2 cut(s)	1161, 1696
AccII	CGCG	2 cut(s)	185, 296
AciI	CCGC	6 cut(s)	212, 294, 296, 487, 490, 562
AclI	AACGTT	2 cut(s)	1286, 1352
AclWI	GGATC	4 cut(s)	76, 652, 1504, 1757
AcoI	YGGCCR	3 cut(s)	168, 2017, 2074
AcsI	RAATTY	3 cut(s)	235, 814, 976
AcuI	CTGAAG	2 cut(s)	369, 1630
AcyI	GRCGYC	1 cut(s)	66
AfaI	GTAC	6 cut(s)	311, 323, 356, 439, 495, 1664
AfeI	AGCGCT	1 cut(s)	605
AfiI	CCNNNNNNNGG	8 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529
AflII	CTTAAG	1 cut(s)	1421
AflIII	ACRYGT	1 cut(s)	1852
AgsI	TTSAA	9 cut(s)	139, 240, 632, 881, 1369, 1559, 1958, 2034, 2112
AjnI	CCWGG	3 cut(s)	799, 1174, 1378
AloI	GAACNNNNNNTCC	4 cut(s)	606, 638, 1028, 1060
AluBI	AGCT	5 cut(s)	316, 716, 1394, 1415, 1563
AluI	AGCT	5 cut(s)	316, 716, 1394, 1415, 1563
Alw26I	GTCTC	1 cut(s)	723
AlwI	GGATC	4 cut(s)	76, 652, 1504, 1757
Aor51HI	AGCGCT	1 cut(s)	605
AoxI	GGCC	6 cut(s)	168, 479, 796, 1212, 2017, 2074
ApaI	GGGCCC	1 cut(s)	800
ApeKI	GCWGC	5 cut(s)	844, 922, 1394, 1412, 1729
ApoI	RAATTY	3 cut(s)	235, 814, 976
AseI	ATTAAT	2 cut(s)	1113, 1836
Asp700I	GAANNNNTTC	1 cut(s)	2029
Asp718I	GGTACC	2 cut(s)	437, 493
AspLEI	GCGC	2 cut(s)	185, 606
AspS9I	GGNCC	5 cut(s)	479, 671, 796, 797, 1213
AsuC2I	CCSGG	1 cut(s)	570
AsuHPI	GGTGA	9 cut(s)	22, 143, 206, 584, 933, 1271, 1300, 1838, 1979
AsuII	TTCGAA	2 cut(s)	262, 1047
AvaII	GGWCC	1 cut(s)	671
BaeGI	GKGCMC	1 cut(s)	800
BanI	GGYRCC	4 cut(s)	437, 493, 1018, 1890
BanII	GRGCYC	2 cut(s)	800, 1814
BbsI	GAAGAC	2 cut(s)	122, 1589
BbvCI	CCTCAGC	1 cut(s)	483
BbvI	GCAGC	5 cut(s)	831, 934, 1381, 1424, 1716
BccI	CCATC	2 cut(s)	41, 866
BceAI	ACGGC	2 cut(s)	155, 2089
BcgI	CGANNNNNNTGC	4 cut(s)	242, 276, 1376, 1410
BciT130I	CCWGG	3 cut(s)	801, 1176, 1380
BciVI	GTATCC	2 cut(s)	734, 2060
BclI	TGATCA	2 cut(s)	1786, 1936
BcnI	CCSGG	1 cut(s)	570
BcoDI	GTCTC	1 cut(s)	723
BfaI	CTAG	2 cut(s)	177, 848
BfoI	RGCGCY	1 cut(s)	607
BfrI	CTTAAG	1 cut(s)	1421
BfuI	GTATCC	2 cut(s)	734, 2060
BglI	GCCNNNNNGGC	1 cut(s)	487
BisI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 1730
BlsI	GCNGC	7 cut(s)	298, 489, 846, 924, 1396, 1414, 1731
BmcAI	AGTACT	1 cut(s)	356
Bme1390I	CCNGG	4 cut(s)	570, 801, 1176, 1380
Bme18I	GGWCC	1 cut(s)	671
BmgT120I	GGNCC	5 cut(s)	479, 671, 796, 797, 1213
BmiI	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
BmrFI	CCNGG	4 cut(s)	570, 801, 1176, 1380
BmsI	GCATC	8 cut(s)	240, 376, 382, 831, 1072, 1200, 1280, 1640
BpiI	GAAGAC	2 cut(s)	122, 1589
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	970
Bpu10I	CCTNAGC	1 cut(s)	483
Bpu14I	TTCGAA	2 cut(s)	262, 1047
BpuEI	CTTGAG	1 cut(s)	1282
BpuMI	CCSGG	1 cut(s)	570
BsaAI	YACGTR	1 cut(s)	321
BsaHI	GRCGYC	1 cut(s)	66
BsaJI	CCNNGG	6 cut(s)	294, 785, 799, 1175, 1378, 2071
BsaWI	WCCGGW	1 cut(s)	128
BsaXI	ACNNNNNCTCC	4 cut(s)	720, 750, 2020, 2050
Bsc4I	CCNNNNNNNGG	8 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529
Bse1I	ACTGG	5 cut(s)	1261, 1543, 1609, 1781, 2068
BseBI	CCWGG	3 cut(s)	801, 1176, 1380
BseDI	CCNNGG	6 cut(s)	294, 785, 799, 1175, 1378, 2071
BseGI	GGATG	5 cut(s)	33, 846, 1063, 1271, 1789
BseLI	CCNNNNNNNGG	8 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529
BseMII	CTCAG	2 cut(s)	497, 621
BseNI	ACTGG	5 cut(s)	1261, 1543, 1609, 1781, 2068
BseRI	GAGGAG	3 cut(s)	29, 245, 1334
BseSI	GKGCMC	1 cut(s)	800
BseXI	GCAGC	5 cut(s)	831, 934, 1381, 1424, 1716
BsgI	GTGCAG	1 cut(s)	1431
Bsh1236I	CGCG	2 cut(s)	185, 296
BshFI	GGCC	6 cut(s)	170, 481, 798, 1214, 2019, 2076
BshNI	GGYRCC	4 cut(s)	437, 493, 1018, 1890
BsiSI	CCGG	2 cut(s)	129, 569
BslFI	GGGAC	3 cut(s)	158, 1090, 1767
BslI	CCNNNNNNNGG	8 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529
BsmAI	GTCTC	1 cut(s)	723
BsmFI	GGGAC	3 cut(s)	158, 1090, 1767
BsnI	GGCC	6 cut(s)	170, 481, 798, 1214, 2019, 2076
Bsp119I	TTCGAA	2 cut(s)	262, 1047
Bsp120I	GGGCCC	1 cut(s)	796
Bsp1286I	GDGCHC	2 cut(s)	800, 1814
BspACI	CCGC	6 cut(s)	212, 294, 296, 487, 490, 562
BspANI	GGCC	6 cut(s)	170, 481, 798, 1214, 2019, 2076
BspCNI	CTCAG	2 cut(s)	496, 620
BspFNI	CGCG	2 cut(s)	185, 296
BspLI	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
BspPI	GGATC	4 cut(s)	76, 652, 1504, 1757
BspQI	GCTCTTC	1 cut(s)	943
BspT104I	TTCGAA	2 cut(s)	262, 1047
BspT107I	GGYRCC	4 cut(s)	437, 493, 1018, 1890
BspTI	CTTAAG	1 cut(s)	1421
BsrI	ACTGG	5 cut(s)	1261, 1543, 1609, 1781, 2068
BssECI	CCNNGG	6 cut(s)	294, 785, 799, 1175, 1378, 2071
BssNAI	GTATAC	1 cut(s)	1697
BssNI	GRCGYC	1 cut(s)	66
Bst1107I	GTATAC	1 cut(s)	1697
Bst2UI	CCWGG	3 cut(s)	801, 1176, 1380
Bst4CI	ACNGT	7 cut(s)	283, 557, 1001, 1483, 1662, 1720, 1934
Bst6I	CTCTTC	3 cut(s)	47, 345, 943
BstACI	GRCGYC	1 cut(s)	66
BstAFI	CTTAAG	1 cut(s)	1421
BstBAI	YACGTR	1 cut(s)	321
BstBI	TTCGAA	2 cut(s)	262, 1047
BstC8I	GCNNGC	2 cut(s)	714, 959
BstDEI	CTNAG	4 cut(s)	147, 483, 607, 1136
BstDSI	CCRYGG	2 cut(s)	294, 2071
BstENI	CCTNNNNNAGG	1 cut(s)	1316
BstF5I	GGATG	5 cut(s)	33, 846, 1063, 1271, 1789
BstFNI	CGCG	2 cut(s)	185, 296
BstH2I	RGCGCY	1 cut(s)	607
BstHHI	GCGC	2 cut(s)	185, 606
BstMAI	GTCTC	1 cut(s)	723
BstMWI	GCNNNNNNNGC	4 cut(s)	302, 457, 487, 1735
BstNI	CCWGG	3 cut(s)	801, 1176, 1380
BstNSI	RCATGY	1 cut(s)	1856
BstSCI	CCNGG	4 cut(s)	568, 799, 1174, 1378
BstSLI	GKGCMC	1 cut(s)	800
BstSNI	TACGTA	1 cut(s)	321
BstUI	CGCG	2 cut(s)	185, 296
BstV1I	GCAGC	5 cut(s)	831, 934, 1381, 1424, 1716
BstV2I	GAAGAC	2 cut(s)	122, 1589
BstX2I	RGATCY	2 cut(s)	81, 644
BstXI	CCANNNNNNTGG	3 cut(s)	24, 590, 1901
BstYI	RGATCY	2 cut(s)	81, 644
BstZ17I	GTATAC	1 cut(s)	1697
BsuI	GTATCC	2 cut(s)	734, 2060
BsuRI	GGCC	6 cut(s)	170, 481, 798, 1214, 2019, 2076
BtgI	CCRYGG	2 cut(s)	294, 2071
BtsCI	GGATG	5 cut(s)	33, 846, 1063, 1271, 1789
BtsIMutI	CAGTG	5 cut(s)	553, 615, 1602, 1939, 2061
Cac8I	GCNNGC	2 cut(s)	714, 959
CfoI	GCGC	2 cut(s)	185, 606
Cfr13I	GGNCC	5 cut(s)	479, 671, 796, 797, 1213
Cfr42I	CCGCGG	1 cut(s)	297
Csp6I	GTAC	6 cut(s)	310, 322, 355, 438, 494, 1663
CspCI	CAANNNNNGTGG	1 cut(s)	38
CviQI	GTAC	6 cut(s)	310, 322, 355, 438, 494, 1663
DdeI	CTNAG	4 cut(s)	147, 483, 607, 1136
EaeI	YGGCCR	3 cut(s)	168, 2017, 2074
Eam1104I	CTCTTC	3 cut(s)	47, 345, 943
EarI	CTCTTC	3 cut(s)	47, 345, 943
Eco105I	TACGTA	1 cut(s)	321
Eco24I	GRGCYC	2 cut(s)	800, 1814
Eco47I	GGWCC	1 cut(s)	671
Eco47III	AGCGCT	1 cut(s)	605
Eco57I	CTGAAG	2 cut(s)	369, 1630
EcoNI	CCTNNNNNAGG	1 cut(s)	1316
EcoO109I	RGGNCCY	3 cut(s)	671, 796, 1213
EcoRI	GAATTC	1 cut(s)	235
EcoRII	CCWGG	3 cut(s)	799, 1174, 1378
EcoT38I	GRGCYC	2 cut(s)	800, 1814
FaqI	GGGAC	3 cut(s)	158, 1090, 1767
FauI	CCCGC	1 cut(s)	483
FauNDI	CATATG	1 cut(s)	1667
FbaI	TGATCA	2 cut(s)	1786, 1936
FblI	GTMKAC	2 cut(s)	1161, 1696
Fnu4HI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 1730
FokI	GGATG	5 cut(s)	20, 853, 1050, 1258, 1796
FriOI	GRGCYC	2 cut(s)	800, 1814
Fsp4HI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 1730
FspBI	CTAG	2 cut(s)	177, 848
GlaI	GCGC	2 cut(s)	184, 605
GluI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 1730
GsuI	CTGGAG	1 cut(s)	970
HaeII	RGCGCY	1 cut(s)	607
HaeIII	GGCC	6 cut(s)	170, 481, 798, 1214, 2019, 2076
HapII	CCGG	2 cut(s)	129, 569
HhaI	GCGC	2 cut(s)	185, 606
Hin1I	GRCGYC	1 cut(s)	66
Hin6I	GCGC	2 cut(s)	183, 604
HinP1I	GCGC	2 cut(s)	183, 604
HincII	GTYRAC	2 cut(s)	806, 901
HindII	GTYRAC	2 cut(s)	806, 901
HindIII	AAGCTT	1 cut(s)	1561
HpaII	CCGG	2 cut(s)	129, 569
HphI	GGTGA	9 cut(s)	22, 143, 206, 584, 933, 1271, 1300, 1838, 1979
Hpy166II	GTNNAC	4 cut(s)	806, 901, 1162, 1697
Hpy188I	TCNGA	8 cut(s)	74, 124, 658, 786, 1649, 1686, 1749, 1809
Hpy188III	TCNNGA	8 cut(s)	79, 277, 329, 674, 949, 1126, 1359, 1632
Hpy8I	GTNNAC	4 cut(s)	806, 901, 1162, 1697
Hpy99I	CGWCG	3 cut(s)	68, 71, 74
HpyAV	CCTTC	8 cut(s)	507, 765, 816, 1031, 1035, 1744, 1994, 2019
HpyCH4III	ACNGT	7 cut(s)	283, 557, 1001, 1483, 1662, 1720, 1934
HpyCH4IV	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
HpyF10VI	GCNNNNNNNGC	4 cut(s)	302, 457, 487, 1735
HpyF3I	CTNAG	4 cut(s)	147, 483, 607, 1136
HpySE526I	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
Hsp92I	GRCGYC	1 cut(s)	66
HspAI	GCGC	2 cut(s)	183, 604
KpnI	GGTACC	2 cut(s)	441, 497
Ksp22I	TGATCA	2 cut(s)	1786, 1936
KspI	CCGCGG	1 cut(s)	297
LguI	GCTCTTC	1 cut(s)	943
LmnI	GCTCC	1 cut(s)	1321
Lsp1109I	GCAGC	5 cut(s)	831, 934, 1381, 1424, 1716
LweI	GCATC	8 cut(s)	240, 376, 382, 831, 1072, 1200, 1280, 1640
MaeI	CTAG	2 cut(s)	177, 848
MaeII	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
MaeIII	GTNAC	4 cut(s)	131, 1099, 1306, 1967
MboII	GAAGA	9 cut(s)	34, 127, 163, 259, 362, 418, 930, 1594, 2034
MflI	RGATCY	2 cut(s)	81, 644
MhlI	GDGCHC	2 cut(s)	800, 1814
MlyI	GAGTC	7 cut(s)	84, 361, 1072, 1107, 1180, 1421, 1544
MmeI	TCCRAC	4 cut(s)	52, 718, 1658, 1739
MroXI	GAANNNNTTC	1 cut(s)	2029
MseI	TTAA	7 cut(s)	363, 915, 1113, 1283, 1422, 1515, 1836
MslI	CAYNNNNRTG	8 cut(s)	1068, 1313, 1670, 1851, 1857, 1878, 1899, 2013
MspA1I	CMGCKG	2 cut(s)	296, 487
MspCI	CTTAAG	1 cut(s)	1421
MspI	CCGG	2 cut(s)	129, 569
MspR9I	CCNGG	4 cut(s)	570, 801, 1176, 1380
MvaI	CCWGG	3 cut(s)	801, 1176, 1380
MvnI	CGCG	2 cut(s)	185, 296
MwoI	GCNNNNNNNGC	4 cut(s)	302, 457, 487, 1735
NciI	CCSGG	1 cut(s)	570
NdeI	CATATG	1 cut(s)	1667
NlaIV	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
NmeAIII	GCCGAG	1 cut(s)	1480
NmuCI	GTSAC	4 cut(s)	131, 1099, 1306, 1967
NspI	RCATGY	1 cut(s)	1856
NspV	TTCGAA	2 cut(s)	262, 1047
PciI	ACATGT	1 cut(s)	1852
PciSI	GCTCTTC	1 cut(s)	943
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PdmI	GAANNNNTTC	1 cut(s)	2029
PfeI	GAWTC	4 cut(s)	6, 125, 1635, 1681
PflFI	GACNNNGTC	1 cut(s)	68
PflMI	CCANNNNNTGG	2 cut(s)	817, 1028
PkrI	GCNGC	7 cut(s)	298, 489, 846, 924, 1396, 1414, 1731
PleI	GAGTC	7 cut(s)	83, 361, 1072, 1106, 1179, 1421, 1544
PpsI	GAGTC	7 cut(s)	83, 361, 1072, 1106, 1179, 1421, 1544
Ppu21I	YACGTR	1 cut(s)	321
PpuMI	RGGWCCY	1 cut(s)	671
PscI	ACATGT	1 cut(s)	1852
PshBI	ATTAAT	2 cut(s)	1113, 1836
PsiI	TTATAA	1 cut(s)	1437
Psp1406I	AACGTT	2 cut(s)	1286, 1352
Psp5II	RGGWCCY	1 cut(s)	671
Psp6I	CCWGG	3 cut(s)	799, 1174, 1378
PspGI	CCWGG	3 cut(s)	799, 1174, 1378
PspN4I	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
PspOMI	GGGCCC	1 cut(s)	796
PspPI	GGNCC	5 cut(s)	479, 671, 796, 797, 1213
PspPPI	RGGWCCY	1 cut(s)	671
PsrI	GAACNNNNNNTAC	2 cut(s)	2051, 2083
PsuI	RGATCY	2 cut(s)	81, 644
PsyI	GACNNNGTC	1 cut(s)	68
RsaI	GTAC	6 cut(s)	311, 323, 356, 439, 495, 1664
RsaNI	GTAC	6 cut(s)	310, 322, 355, 438, 494, 1663
RseI	CAYNNNNRTG	8 cut(s)	1068, 1313, 1670, 1851, 1857, 1878, 1899, 2013
SacII	CCGCGG	1 cut(s)	297
SapI	GCTCTTC	1 cut(s)	943
SaqAI	TTAA	7 cut(s)	363, 915, 1113, 1283, 1422, 1515, 1836
SatI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 1730
Sau96I	GGNCC	5 cut(s)	479, 671, 796, 797, 1213
ScaI	AGTACT	1 cut(s)	356
SchI	GAGTC	7 cut(s)	84, 361, 1072, 1107, 1180, 1421, 1544
ScrFI	CCNGG	4 cut(s)	570, 801, 1176, 1380
SduI	GDGCHC	2 cut(s)	800, 1814
SfaNI	GCATC	8 cut(s)	240, 376, 382, 831, 1072, 1200, 1280, 1640
Sfr303I	CCGCGG	1 cut(s)	297
SfuI	TTCGAA	2 cut(s)	262, 1047
SgrBI	CCGCGG	1 cut(s)	297
SinI	GGWCC	1 cut(s)	671
SmiMI	CAYNNNNRTG	8 cut(s)	1068, 1313, 1670, 1851, 1857, 1878, 1899, 2013
SmlI	CTYRAG	2 cut(s)	1297, 1421
SmoI	CTYRAG	2 cut(s)	1297, 1421
SnaBI	TACGTA	1 cut(s)	321
SsiI	CCGC	6 cut(s)	212, 294, 296, 487, 490, 562
SspI	AATATT	1 cut(s)	751
SspMI	CTAG	2 cut(s)	177, 848
StyD4I	CCNGG	4 cut(s)	568, 799, 1174, 1378
TaaI	ACNGT	7 cut(s)	283, 557, 1001, 1483, 1662, 1720, 1934
TaiI	ACGT	5 cut(s)	69, 323, 1152, 1289, 1355
TatI	WGTACW	2 cut(s)	354, 1662
TauI	GCSGC	2 cut(s)	299, 490
TfiI	GAWTC	4 cut(s)	6, 125, 1635, 1681
Tru1I	TTAA	7 cut(s)	363, 915, 1113, 1283, 1422, 1515, 1836
Tru9I	TTAA	7 cut(s)	363, 915, 1113, 1283, 1422, 1515, 1836
TscAI	CASTG	5 cut(s)	560, 615, 1609, 1939, 2068
TseFI	GTSAC	4 cut(s)	131, 1099, 1306, 1967
TseI	GCWGC	5 cut(s)	844, 922, 1394, 1412, 1729
Tsp45I	GTSAC	4 cut(s)	131, 1099, 1306, 1967
TspRI	CASTG	5 cut(s)	560, 615, 1609, 1939, 2068
Tth111I	GACNNNGTC	1 cut(s)	68
Van91I	CCANNNNNTGG	2 cut(s)	817, 1028
Vha464I	CTTAAG	1 cut(s)	1421
VpaK11BI	GGWCC	1 cut(s)	671
VspI	ATTAAT	2 cut(s)	1113, 1836
XagI	CCTNNNNNAGG	1 cut(s)	1316
XapI	RAATTY	3 cut(s)	235, 814, 976
XceI	RCATGY	1 cut(s)	1856
XcmI	CCANNNNNNNNNTGG	3 cut(s)	21, 1172, 1871
XmiI	GTMKAC	2 cut(s)	1161, 1696
XmnI	GAANNNNTTC	1 cut(s)	2029
XspI	CTAG	2 cut(s)	177, 848
ZraI	GACGTC	1 cut(s)	67
ZrmI	AGTACT	1 cut(s)	356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.