Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

15509202 .. 15515320

Gene Structure 6119 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G015420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGAGAATCCCTCCACCAAAATTATGGCTCACCATCCTCCTCTTCCTCCCATGCTTCATCAGCGACGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTCCGACTCCGGTGACTTGAATTGCACTAAGAAGATCGTCCTCAACATGGCTGTCCCCAGTGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAGATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCGCCTGTCCTGACTGTCAATAAATCCGCGGCTTATGCCTTGTACGAGCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACTCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTGAAGATATGTGAGAGGTTGCGAGATGAAAATGGTACCATTCTTGAGCATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCATGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCCAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGGATCTAAAGTTTCAAAAGTGCTTGTAGGTCCTGAAAACCAAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGACTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAGTTATGGAATTTCTTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGGAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTTATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAGAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGCTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAACGTTATCTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGGGCCGAGTGCCAATTTTCCACGACATCTACTGTCATTGATAATGGATCACAGGGTAATCCCTTTAAGCCACCAAAGGCAGGAACAACTGGTTTCTTTGACTCCATTGAAAGCTTTTGGAACCATGTGTGGGAGACTTTTATAGATTTCATCACCGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTTGGTCTACTTTTAGCAATTTTTCCAACAGTCATTGTGCTACTGTGGCTTTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAGGATAGTCCGAGCACCAAGAATACAAGACACAGAATTAATGTAGATCACCGACATGTTCATGTCCATAAACATCATGGGCATGAAGCAAGGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGACTACCATTACGACCTTCACCATGTTCATAAGGATAAGCACAAGCATGGCCGAAGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCGCGGCCAGAAAAAACAAAGGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

705

Amino Acids

80.52

Weight (kDa)

8.49

Isoelectric Point (pI)

40.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	46 - 558	4.1e-159	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1437
AatII	GACGTC	1 cut(s)	69
Acc65I	GGTACC	2 cut(s)	437, 493
AccB1I	GGYRCC	4 cut(s)	437, 493, 1018, 1890
AccB7I	CCANNNNNTGG	2 cut(s)	817, 1028
AccI	GTMKAC	2 cut(s)	1161, 1696
AccII	CGCG	3 cut(s)	185, 296, 2073
AciI	CCGC	9 cut(s)	212, 269, 294, 296, 487, 490, 562, 2071, 2073
AclI	AACGTT	2 cut(s)	1286, 1352
AclWI	GGATC	4 cut(s)	76, 652, 1504, 1757
AcoI	YGGCCR	2 cut(s)	2017, 2074
AcsI	RAATTY	3 cut(s)	235, 814, 976
AcuI	CTGAAG	2 cut(s)	369, 1630
AcyI	GRCGYC	1 cut(s)	66
AfaI	GTAC	6 cut(s)	311, 323, 356, 439, 495, 1664
AfeI	AGCGCT	1 cut(s)	605
AfiI	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
AflII	CTTAAG	2 cut(s)	1186, 1421
AflIII	ACRYGT	1 cut(s)	1852
AjnI	CCWGG	3 cut(s)	799, 1174, 1378
AloI	GAACNNNNNNTCC	4 cut(s)	606, 638, 1028, 1060
AluBI	AGCT	5 cut(s)	316, 716, 1394, 1415, 1563
AluI	AGCT	5 cut(s)	316, 716, 1394, 1415, 1563
Alw21I	GWGCWC	1 cut(s)	1814
Alw26I	GTCTC	2 cut(s)	723, 1577
AlwI	GGATC	4 cut(s)	76, 652, 1504, 1757
Aor51HI	AGCGCT	1 cut(s)	605
AoxI	GGCC	6 cut(s)	479, 796, 1212, 1452, 2017, 2074
ApaI	GGGCCC	1 cut(s)	800
ApeKI	GCWGC	4 cut(s)	844, 922, 1394, 1412
ApoI	RAATTY	3 cut(s)	235, 814, 976
AseI	ATTAAT	2 cut(s)	1113, 1836
Asp700I	GAANNNNTTC	1 cut(s)	2029
Asp718I	GGTACC	2 cut(s)	437, 493
AspLEI	GCGC	2 cut(s)	185, 606
AspS9I	GGNCC	6 cut(s)	479, 671, 796, 797, 1213, 1452
AsuC2I	CCSGG	1 cut(s)	570
AsuII	TTCGAA	2 cut(s)	262, 1047
AvaII	GGWCC	1 cut(s)	671
BaeGI	GKGCMC	1 cut(s)	800
BanI	GGYRCC	4 cut(s)	437, 493, 1018, 1890
BanII	GRGCYC	1 cut(s)	800
BbsI	GAAGAC	1 cut(s)	122
Bbv12I	GWGCWC	1 cut(s)	1814
BbvCI	CCTCAGC	1 cut(s)	483
BbvI	GCAGC	4 cut(s)	831, 934, 1381, 1424
BccI	CCATC	2 cut(s)	41, 866
BcgI	CGANNNNNNTGC	4 cut(s)	242, 276, 1376, 1410
BciT130I	CCWGG	3 cut(s)	801, 1176, 1380
BciVI	GTATCC	2 cut(s)	734, 2060
BclI	TGATCA	2 cut(s)	1786, 1936
BcnI	CCSGG	1 cut(s)	570
BcoDI	GTCTC	2 cut(s)	723, 1577
BfoI	RGCGCY	1 cut(s)	607
BfrI	CTTAAG	2 cut(s)	1186, 1421
BfuI	GTATCC	2 cut(s)	734, 2060
BglI	GCCNNNNNGGC	1 cut(s)	487
BisI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 2074
BlsI	GCNGC	7 cut(s)	298, 489, 846, 924, 1396, 1414, 2075
BmcAI	AGTACT	1 cut(s)	356
Bme1390I	CCNGG	4 cut(s)	570, 801, 1176, 1380
Bme18I	GGWCC	1 cut(s)	671
BmgT120I	GGNCC	6 cut(s)	479, 671, 796, 797, 1213, 1452
BmiI	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
BmrFI	CCNGG	4 cut(s)	570, 801, 1176, 1380
BmrI	ACTGGG	1 cut(s)	171
BmsI	GCATC	8 cut(s)	240, 376, 382, 831, 1072, 1200, 1280, 1640
BmuI	ACTGGG	1 cut(s)	171
BpiI	GAAGAC	1 cut(s)	122
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	2 cut(s)	869, 970
Bpu10I	CCTNAGC	1 cut(s)	483
Bpu14I	TTCGAA	2 cut(s)	262, 1047
BpuEI	CTTGAG	2 cut(s)	467, 1282
BpuMI	CCSGG	1 cut(s)	570
BsaAI	YACGTR	1 cut(s)	321
BsaHI	GRCGYC	1 cut(s)	66
BsaJI	CCNNGG	6 cut(s)	294, 785, 799, 1175, 1378, 2071
BsaWI	WCCGGW	2 cut(s)	128, 1604
BsaXI	ACNNNNNCTCC	4 cut(s)	720, 750, 2020, 2050
Bsc4I	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
Bse1I	ACTGG	5 cut(s)	177, 1261, 1543, 1781, 2068
BseBI	CCWGG	3 cut(s)	801, 1176, 1380
BseDI	CCNNGG	6 cut(s)	294, 785, 799, 1175, 1378, 2071
BseGI	GGATG	5 cut(s)	33, 846, 1063, 1271, 1789
BseLI	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
BseMII	CTCAG	2 cut(s)	497, 621
BseNI	ACTGG	5 cut(s)	177, 1261, 1543, 1781, 2068
BseRI	GAGGAG	3 cut(s)	29, 245, 1334
BseSI	GKGCMC	1 cut(s)	800
BseXI	GCAGC	4 cut(s)	831, 934, 1381, 1424
BsgI	GTGCAG	1 cut(s)	1431
Bsh1236I	CGCG	3 cut(s)	185, 296, 2073
BshFI	GGCC	6 cut(s)	481, 798, 1214, 1454, 2019, 2076
BshNI	GGYRCC	4 cut(s)	437, 493, 1018, 1890
BsiHKAI	GWGCWC	1 cut(s)	1814
BsiSI	CCGG	3 cut(s)	129, 569, 1605
BslFI	GGGAC	3 cut(s)	158, 1090, 1767
BslI	CCNNNNNNNGG	9 cut(s)	166, 568, 791, 817, 1028, 1073, 1318, 1529, 1579
BsmAI	GTCTC	2 cut(s)	723, 1577
BsmFI	GGGAC	3 cut(s)	158, 1090, 1767
BsnI	GGCC	6 cut(s)	481, 798, 1214, 1454, 2019, 2076
Bsp119I	TTCGAA	2 cut(s)	262, 1047
Bsp120I	GGGCCC	1 cut(s)	796
Bsp1286I	GDGCHC	2 cut(s)	800, 1814
BspACI	CCGC	9 cut(s)	212, 269, 294, 296, 487, 490, 562, 2071, 2073
BspANI	GGCC	6 cut(s)	481, 798, 1214, 1454, 2019, 2076
BspCNI	CTCAG	2 cut(s)	496, 620
BspFNI	CGCG	3 cut(s)	185, 296, 2073
BspLI	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
BspPI	GGATC	4 cut(s)	76, 652, 1504, 1757
BspQI	GCTCTTC	1 cut(s)	943
BspT104I	TTCGAA	2 cut(s)	262, 1047
BspT107I	GGYRCC	4 cut(s)	437, 493, 1018, 1890
BspTI	CTTAAG	2 cut(s)	1186, 1421
BsrI	ACTGG	5 cut(s)	177, 1261, 1543, 1781, 2068
BssECI	CCNNGG	6 cut(s)	294, 785, 799, 1175, 1378, 2071
BssNI	GRCGYC	1 cut(s)	66
Bst2UI	CCWGG	3 cut(s)	801, 1176, 1380
Bst4CI	ACNGT	6 cut(s)	283, 1001, 1483, 1662, 1720, 1734
Bst6I	CTCTTC	3 cut(s)	47, 345, 943
BstACI	GRCGYC	1 cut(s)	66
BstAFI	CTTAAG	2 cut(s)	1186, 1421
BstBAI	YACGTR	1 cut(s)	321
BstBI	TTCGAA	2 cut(s)	262, 1047
BstC8I	GCNNGC	2 cut(s)	714, 959
BstDEI	CTNAG	4 cut(s)	147, 483, 607, 1136
BstDSI	CCRYGG	2 cut(s)	294, 2071
BstENI	CCTNNNNNAGG	1 cut(s)	1316
BstF5I	GGATG	5 cut(s)	33, 846, 1063, 1271, 1789
BstFNI	CGCG	3 cut(s)	185, 296, 2073
BstH2I	RGCGCY	1 cut(s)	607
BstHHI	GCGC	2 cut(s)	185, 606
BstMAI	GTCTC	2 cut(s)	723, 1577
BstMWI	GCNNNNNNNGC	5 cut(s)	60, 302, 457, 487, 1735
BstNI	CCWGG	3 cut(s)	801, 1176, 1380
BstNSI	RCATGY	1 cut(s)	1856
BstSCI	CCNGG	4 cut(s)	568, 799, 1174, 1378
BstSLI	GKGCMC	1 cut(s)	800
BstSNI	TACGTA	1 cut(s)	321
BstUI	CGCG	3 cut(s)	185, 296, 2073
BstV1I	GCAGC	4 cut(s)	831, 934, 1381, 1424
BstV2I	GAAGAC	1 cut(s)	122
BstX2I	RGATCY	2 cut(s)	81, 644
BstXI	CCANNNNNNTGG	3 cut(s)	24, 590, 1901
BstYI	RGATCY	2 cut(s)	81, 644
BsuI	GTATCC	2 cut(s)	734, 2060
BsuRI	GGCC	6 cut(s)	481, 798, 1214, 1454, 2019, 2076
BtgI	CCRYGG	2 cut(s)	294, 2071
BtsCI	GGATG	5 cut(s)	33, 846, 1063, 1271, 1789
BtsIMutI	CAGTG	3 cut(s)	184, 615, 2061
Cac8I	GCNNGC	2 cut(s)	714, 959
CfoI	GCGC	2 cut(s)	185, 606
Cfr13I	GGNCC	6 cut(s)	479, 671, 796, 797, 1213, 1452
Cfr42I	CCGCGG	2 cut(s)	297, 2074
Csp6I	GTAC	6 cut(s)	310, 322, 355, 438, 494, 1663
CviQI	GTAC	6 cut(s)	310, 322, 355, 438, 494, 1663
DdeI	CTNAG	4 cut(s)	147, 483, 607, 1136
EaeI	YGGCCR	2 cut(s)	2017, 2074
Eam1104I	CTCTTC	3 cut(s)	47, 345, 943
EarI	CTCTTC	3 cut(s)	47, 345, 943
Eco105I	TACGTA	1 cut(s)	321
Eco24I	GRGCYC	1 cut(s)	800
Eco47I	GGWCC	1 cut(s)	671
Eco47III	AGCGCT	1 cut(s)	605
Eco57I	CTGAAG	2 cut(s)	369, 1630
EcoNI	CCTNNNNNAGG	1 cut(s)	1316
EcoO109I	RGGNCCY	3 cut(s)	671, 796, 1213
EcoRI	GAATTC	1 cut(s)	235
EcoRII	CCWGG	3 cut(s)	799, 1174, 1378
EcoT38I	GRGCYC	1 cut(s)	800
FaqI	GGGAC	3 cut(s)	158, 1090, 1767
FauI	CCCGC	1 cut(s)	483
FauNDI	CATATG	1 cut(s)	1667
FbaI	TGATCA	2 cut(s)	1786, 1936
FblI	GTMKAC	2 cut(s)	1161, 1696
Fnu4HI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 2074
FokI	GGATG	5 cut(s)	20, 853, 1050, 1258, 1796
FriOI	GRGCYC	1 cut(s)	800
Fsp4HI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 2074
GlaI	GCGC	2 cut(s)	184, 605
GluI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 2074
GsuI	CTGGAG	2 cut(s)	869, 970
HaeII	RGCGCY	1 cut(s)	607
HaeIII	GGCC	6 cut(s)	481, 798, 1214, 1454, 2019, 2076
HapII	CCGG	3 cut(s)	129, 569, 1605
HhaI	GCGC	2 cut(s)	185, 606
Hin1I	GRCGYC	1 cut(s)	66
Hin6I	GCGC	2 cut(s)	183, 604
HinP1I	GCGC	2 cut(s)	183, 604
HincII	GTYRAC	2 cut(s)	806, 901
HindII	GTYRAC	2 cut(s)	806, 901
HindIII	AAGCTT	1 cut(s)	1561
HpaII	CCGG	3 cut(s)	129, 569, 1605
Hpy166II	GTNNAC	4 cut(s)	806, 901, 1162, 1697
Hpy188I	TCNGA	6 cut(s)	124, 786, 1649, 1686, 1749, 1809
Hpy188III	TCNNGA	9 cut(s)	79, 277, 329, 446, 674, 949, 1126, 1359, 1632
Hpy8I	GTNNAC	4 cut(s)	806, 901, 1162, 1697
Hpy99I	CGWCG	2 cut(s)	68, 71
HpyAV	CCTTC	7 cut(s)	507, 765, 816, 1031, 1744, 1994, 2019
HpyCH4III	ACNGT	6 cut(s)	283, 1001, 1483, 1662, 1720, 1734
HpyCH4IV	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
HpyF10VI	GCNNNNNNNGC	5 cut(s)	60, 302, 457, 487, 1735
HpyF3I	CTNAG	4 cut(s)	147, 483, 607, 1136
HpySE526I	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
Hsp92I	GRCGYC	1 cut(s)	66
HspAI	GCGC	2 cut(s)	183, 604
KpnI	GGTACC	2 cut(s)	441, 497
Ksp22I	TGATCA	2 cut(s)	1786, 1936
KspI	CCGCGG	2 cut(s)	297, 2074
LguI	GCTCTTC	1 cut(s)	943
LmnI	GCTCC	1 cut(s)	1321
Lsp1109I	GCAGC	4 cut(s)	831, 934, 1381, 1424
LweI	GCATC	8 cut(s)	240, 376, 382, 831, 1072, 1200, 1280, 1640
MaeII	ACGT	5 cut(s)	66, 320, 1149, 1286, 1352
MaeIII	GTNAC	4 cut(s)	131, 1099, 1306, 1967
MboII	GAAGA	8 cut(s)	34, 127, 163, 259, 362, 418, 930, 2034
MflI	RGATCY	2 cut(s)	81, 644
MhlI	GDGCHC	2 cut(s)	800, 1814
MlyI	GAGTC	7 cut(s)	84, 119, 361, 1107, 1180, 1421, 1544
MmeI	TCCRAC	5 cut(s)	52, 147, 718, 1658, 1739
MroXI	GAANNNNTTC	1 cut(s)	2029
MseI	TTAA	8 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515, 1836
MslI	CAYNNNNRTG	8 cut(s)	1068, 1313, 1670, 1851, 1857, 1878, 1899, 2013
MspA1I	CMGCKG	3 cut(s)	296, 487, 2073
MspCI	CTTAAG	2 cut(s)	1186, 1421
MspI	CCGG	3 cut(s)	129, 569, 1605
MspR9I	CCNGG	4 cut(s)	570, 801, 1176, 1380
MvaI	CCWGG	3 cut(s)	801, 1176, 1380
MvnI	CGCG	3 cut(s)	185, 296, 2073
MwoI	GCNNNNNNNGC	5 cut(s)	60, 302, 457, 487, 1735
NciI	CCSGG	1 cut(s)	570
NdeI	CATATG	1 cut(s)	1667
NlaIV	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
NmeAIII	GCCGAG	1 cut(s)	1480
NmuCI	GTSAC	4 cut(s)	131, 1099, 1306, 1967
NspI	RCATGY	1 cut(s)	1856
NspV	TTCGAA	2 cut(s)	262, 1047
PciI	ACATGT	1 cut(s)	1852
PciSI	GCTCTTC	1 cut(s)	943
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PdmI	GAANNNNTTC	1 cut(s)	2029
PfeI	GAWTC	3 cut(s)	6, 1635, 1681
PflMI	CCANNNNNTGG	2 cut(s)	817, 1028
PkrI	GCNGC	7 cut(s)	298, 489, 846, 924, 1396, 1414, 2075
PleI	GAGTC	7 cut(s)	83, 119, 361, 1106, 1179, 1421, 1544
PpsI	GAGTC	7 cut(s)	83, 119, 361, 1106, 1179, 1421, 1544
Ppu21I	YACGTR	1 cut(s)	321
PpuMI	RGGWCCY	1 cut(s)	671
PscI	ACATGT	1 cut(s)	1852
PshBI	ATTAAT	2 cut(s)	1113, 1836
PsiI	TTATAA	1 cut(s)	1437
Psp1406I	AACGTT	2 cut(s)	1286, 1352
Psp5II	RGGWCCY	1 cut(s)	671
Psp6I	CCWGG	3 cut(s)	799, 1174, 1378
PspGI	CCWGG	3 cut(s)	799, 1174, 1378
PspN4I	GGNNCC	9 cut(s)	439, 495, 581, 797, 798, 1020, 1384, 1571, 1892
PspOMI	GGGCCC	1 cut(s)	796
PspPI	GGNCC	6 cut(s)	479, 671, 796, 797, 1213, 1452
PspPPI	RGGWCCY	1 cut(s)	671
PsrI	GAACNNNNNNTAC	2 cut(s)	2051, 2083
PsuI	RGATCY	2 cut(s)	81, 644
RsaI	GTAC	6 cut(s)	311, 323, 356, 439, 495, 1664
RsaNI	GTAC	6 cut(s)	310, 322, 355, 438, 494, 1663
RseI	CAYNNNNRTG	8 cut(s)	1068, 1313, 1670, 1851, 1857, 1878, 1899, 2013
SacII	CCGCGG	2 cut(s)	297, 2074
SapI	GCTCTTC	1 cut(s)	943
SaqAI	TTAA	8 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515, 1836
SatI	GCNGC	7 cut(s)	297, 488, 845, 923, 1395, 1413, 2074
Sau96I	GGNCC	6 cut(s)	479, 671, 796, 797, 1213, 1452
ScaI	AGTACT	1 cut(s)	356
SchI	GAGTC	7 cut(s)	84, 119, 361, 1107, 1180, 1421, 1544
ScrFI	CCNGG	4 cut(s)	570, 801, 1176, 1380
SduI	GDGCHC	2 cut(s)	800, 1814
SfaNI	GCATC	8 cut(s)	240, 376, 382, 831, 1072, 1200, 1280, 1640
Sfr303I	CCGCGG	2 cut(s)	297, 2074
SfuI	TTCGAA	2 cut(s)	262, 1047
SgrBI	CCGCGG	2 cut(s)	297, 2074
SinI	GGWCC	1 cut(s)	671
SmiMI	CAYNNNNRTG	8 cut(s)	1068, 1313, 1670, 1851, 1857, 1878, 1899, 2013
SmlI	CTYRAG	4 cut(s)	446, 1186, 1297, 1421
SmoI	CTYRAG	4 cut(s)	446, 1186, 1297, 1421
SnaBI	TACGTA	1 cut(s)	321
SsiI	CCGC	9 cut(s)	212, 269, 294, 296, 487, 490, 562, 2071, 2073
SspI	AATATT	1 cut(s)	751
StyD4I	CCNGG	4 cut(s)	568, 799, 1174, 1378
TaaI	ACNGT	6 cut(s)	283, 1001, 1483, 1662, 1720, 1734
TaiI	ACGT	5 cut(s)	69, 323, 1152, 1289, 1355
TatI	WGTACW	2 cut(s)	354, 1662
TauI	GCSGC	3 cut(s)	299, 490, 2076
TfiI	GAWTC	3 cut(s)	6, 1635, 1681
Tru1I	TTAA	8 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515, 1836
Tru9I	TTAA	8 cut(s)	363, 915, 1113, 1187, 1283, 1422, 1515, 1836
TscAI	CASTG	3 cut(s)	184, 615, 2068
TseFI	GTSAC	4 cut(s)	131, 1099, 1306, 1967
TseI	GCWGC	4 cut(s)	844, 922, 1394, 1412
Tsp45I	GTSAC	4 cut(s)	131, 1099, 1306, 1967
TspRI	CASTG	3 cut(s)	184, 615, 2068
Van91I	CCANNNNNTGG	2 cut(s)	817, 1028
Vha464I	CTTAAG	2 cut(s)	1186, 1421
VpaK11BI	GGWCC	1 cut(s)	671
VspI	ATTAAT	2 cut(s)	1113, 1836
XagI	CCTNNNNNAGG	1 cut(s)	1316
XapI	RAATTY	3 cut(s)	235, 814, 976
XceI	RCATGY	1 cut(s)	1856
XcmI	CCANNNNNNNNNTGG	3 cut(s)	21, 1172, 1871
XmiI	GTMKAC	2 cut(s)	1161, 1696
XmnI	GAANNNNTTC	1 cut(s)	2029
ZraI	GACGTC	1 cut(s)	67
ZrmI	AGTACT	1 cut(s)	356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.