Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

41287512 .. 41294597

Gene Structure 7086 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG162900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGAGCAGCGATATTCAAAGACAAGAGTCTGAGAAGAATCCGTTGATGAGGTTCTTCAAGCTTGTTCAGCAGCACTTGGCGTCAGGCTCTGATGGTGAAGATAATGGAAGTTCAAAGATTTCAGAGGAGGATTTGGAGACCCCATTTGTTGACTTCTTACAGACACGGCTGCCTCCCAAGATAAAATCGTACAGATCATATCGCATTTCTCTATCTACATTCCCAAAAATGGGAAACCCTCCACCAAAATTATGGCTTATCACCCTCCTCTTCCTCCCTTGCTTCTTCATAAGCGACGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTTGACAAGTGCGAGAAGACTTCCGAATCCGGTGAACTTAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGCGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAAATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCACCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACGAGCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTCAAGATATGTGAGAGGTTACGAGATGAAAATGGTACCATTATTGAACATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCATGTGGAAGTATTTTTGACAAGTTGATGAAAGGAAAACCGAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGCATCCAAAGTTTCGGAAGTGATTGTAGGTCCTGAAAACAGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGATTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCTAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAATTATGGAATTTCTTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAGTAATGTATCAGCAATATTTGAGTTATTTTTCACAAGGAATTTCTGTCATTTGGATTCATATCATTTCTGATTGGTTGCAGAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAAAATACTGGTGAAGTGGAAGCATCCTATTGCTTAACGTTTGATTGTTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAATGTTAACTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGAGCCGAGTGCCAATTTTCTACGACGTCTACTGTCATTGATAATGGATCACAGGTTAATCCCTTTAAGCCACCAAAGACAGGAACAAATGGTTTCTTTGACTCCATTGAAAGCTTTTGGAACCATGCGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTTGGTCTACTTTTGGCAATTTTTCCAACAGTCATTGTGCTACTGTGGCTTTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAGGATAGTCCGAGCACCAAGAATACTAGACACAGAATTAATGTAGATCATCGACATGTTCATGTCCATAAACATCATGGGCATGAAACAATGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGATTACAATTACGACCTTCACCATGTCCATAAGGATAAGCACAAGCATGGCCGAAGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCATGGCCAGAAAAAACAAAGGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

809

Amino Acids

92.75

Weight (kDa)

8.21

Isoelectric Point (pI)

43.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	123 - 662	6.1e-155	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1749
AatII	GACGTC	2 cut(s)	300, 1790
Acc65I	GGTACC	2 cut(s)	668, 724
AccB1I	GGYRCC	3 cut(s)	668, 724, 1249
AccB7I	CCANNNNNTGG	2 cut(s)	1048, 1259
AccI	GTMKAC	3 cut(s)	1473, 1790, 2008
AccII	CGCG	1 cut(s)	416
AciI	CCGC	6 cut(s)	411, 443, 525, 718, 721, 793
AclI	AACGTT	1 cut(s)	1598
AclWI	GGATC	3 cut(s)	307, 1816, 2069
AcoI	YGGCCR	3 cut(s)	399, 2329, 2386
AcsI	RAATTY	4 cut(s)	466, 1045, 1207, 1338
AcuI	CTGAAG	2 cut(s)	600, 1942
AcyI	GRCGYC	3 cut(s)	80, 297, 1787
AfaI	GTAC	7 cut(s)	191, 542, 554, 587, 670, 726, 1976
AfeI	AGCGCT	1 cut(s)	836
AfiI	CCNNNNNNNGG	9 cut(s)	229, 230, 397, 799, 1022, 1048, 1259, 1630, 1841
AflII	CTTAAG	2 cut(s)	1498, 1733
AflIII	ACRYGT	1 cut(s)	2164
AjnI	CCWGG	3 cut(s)	1030, 1486, 1690
AloI	GAACNNNNNNTCC	4 cut(s)	837, 869, 1259, 1291
AluBI	AGCT	7 cut(s)	61, 530, 547, 947, 1706, 1727, 1875
AluI	AGCT	7 cut(s)	61, 530, 547, 947, 1706, 1727, 1875
Alw21I	GWGCWC	1 cut(s)	2126
Alw26I	GTCTC	1 cut(s)	131
AlwI	GGATC	3 cut(s)	307, 1816, 2069
AlwNI	CAGNNNCTG	1 cut(s)	89
Aor51HI	AGCGCT	1 cut(s)	836
AoxI	GGCC	6 cut(s)	399, 710, 1027, 1524, 2329, 2386
ApaI	GGGCCC	1 cut(s)	1031
ApeKI	GCWGC	8 cut(s)	6, 70, 169, 527, 1075, 1153, 1706, 1724
ApoI	RAATTY	4 cut(s)	466, 1045, 1207, 1338
AseI	ATTAAT	2 cut(s)	1425, 2148
Asp700I	GAANNNNTTC	1 cut(s)	2341
Asp718I	GGTACC	2 cut(s)	668, 724
AspA2I	CCTAGG	1 cut(s)	1016
AspLEI	GCGC	2 cut(s)	416, 837
AspS9I	GGNCC	5 cut(s)	710, 902, 1027, 1028, 1525
AsuC2I	CCSGG	1 cut(s)	801
AsuHPI	GGTGA	9 cut(s)	107, 253, 374, 437, 815, 1164, 1583, 1612, 2291
AsuII	TTCGAA	2 cut(s)	493, 1278
AvaII	GGWCC	1 cut(s)	902
AvrII	CCTAGG	1 cut(s)	1016
BaeGI	GKGCMC	1 cut(s)	1031
BalI	TGGCCA	1 cut(s)	2388
BanI	GGYRCC	3 cut(s)	668, 724, 1249
BanII	GRGCYC	1 cut(s)	1031
BbsI	GAAGAC	2 cut(s)	353, 1901
Bbv12I	GWGCWC	1 cut(s)	2126
BbvCI	CCTCAGC	1 cut(s)	714
BbvI	GCAGC	8 cut(s)	18, 82, 156, 539, 1062, 1165, 1693, 1736
BccI	CCATC	2 cut(s)	86, 1097
BceAI	ACGGC	2 cut(s)	182, 386
BcgI	CGANNNNNNTGC	4 cut(s)	473, 507, 1688, 1722
BciT130I	CCWGG	3 cut(s)	1032, 1488, 1692
BciVI	GTATCC	2 cut(s)	965, 2372
BclI	TGATCA	2 cut(s)	2098, 2248
BcnI	CCSGG	1 cut(s)	801
BcoDI	GTCTC	1 cut(s)	131
BfaI	CTAG	3 cut(s)	408, 1017, 2136
BfoI	RGCGCY	1 cut(s)	838
BfrI	CTTAAG	2 cut(s)	1498, 1733
BfuI	GTATCC	2 cut(s)	965, 2372
BglI	GCCNNNNNGGC	1 cut(s)	718
BlnI	CCTAGG	1 cut(s)	1016
BmcAI	AGTACT	1 cut(s)	587
Bme1390I	CCNGG	4 cut(s)	801, 1032, 1488, 1692
Bme18I	GGWCC	1 cut(s)	902
BmgT120I	GGNCC	5 cut(s)	710, 902, 1027, 1028, 1525
BmiI	GGNNCC	8 cut(s)	670, 726, 812, 1028, 1029, 1251, 1696, 1883
BmrFI	CCNGG	4 cut(s)	801, 1032, 1488, 1692
BmsI	GCATC	9 cut(s)	471, 607, 613, 884, 1062, 1303, 1512, 1592, 1952
BoxI	GACNNNNGTC	1 cut(s)	25
BpiI	GAAGAC	2 cut(s)	353, 1901
BpmI	CTGGAG	2 cut(s)	1100, 1201
Bpu10I	CCTNAGC	1 cut(s)	714
Bpu14I	TTCGAA	2 cut(s)	493, 1278
BpuMI	CCSGG	1 cut(s)	801
BsaAI	YACGTR	1 cut(s)	552
BsaBI	GATNNNNATC	1 cut(s)	1358
BsaHI	GRCGYC	3 cut(s)	80, 297, 1787
BsaI	GGTCTC	1 cut(s)	131
BsaJI	CCNNGG	5 cut(s)	1016, 1030, 1487, 1690, 2383
BsaWI	WCCGGW	1 cut(s)	359
BsaXI	ACNNNNNCTCC	4 cut(s)	951, 981, 2332, 2362
Bsc4I	CCNNNNNNNGG	9 cut(s)	229, 230, 397, 799, 1022, 1048, 1259, 1630, 1841
Bse1I	ACTGG	5 cut(s)	1298, 1573, 1921, 2093, 2380
Bse8I	GATNNNNATC	1 cut(s)	1358
BseBI	CCWGG	3 cut(s)	1032, 1488, 1692
BseDI	CCNNGG	5 cut(s)	1016, 1030, 1487, 1690, 2383
BseGI	GGATG	5 cut(s)	875, 1077, 1294, 1583, 2101
BseJI	GATNNNNATC	1 cut(s)	1358
BseLI	CCNNNNNNNGG	9 cut(s)	229, 230, 397, 799, 1022, 1048, 1259, 1630, 1841
BseMII	CTCAG	3 cut(s)	21, 728, 852
BseNI	ACTGG	5 cut(s)	1298, 1573, 1921, 2093, 2380
BseRI	GAGGAG	4 cut(s)	140, 257, 476, 1646
BseSI	GKGCMC	1 cut(s)	1031
BseXI	GCAGC	8 cut(s)	18, 82, 156, 539, 1062, 1165, 1693, 1736
BsgI	GTGCAG	2 cut(s)	358, 1743
Bsh1236I	CGCG	1 cut(s)	416
BshFI	GGCC	6 cut(s)	401, 712, 1029, 1526, 2331, 2388
BshNI	GGYRCC	3 cut(s)	668, 724, 1249
BsiHKAI	GWGCWC	1 cut(s)	2126
BsiSI	CCGG	2 cut(s)	360, 800
BslFI	GGGAC	3 cut(s)	389, 1402, 2079
BslI	CCNNNNNNNGG	9 cut(s)	229, 230, 397, 799, 1022, 1048, 1259, 1630, 1841
BsmAI	GTCTC	1 cut(s)	131
BsmFI	GGGAC	3 cut(s)	389, 1402, 2079
BsmI	GAATGC	1 cut(s)	1387
BsnI	GGCC	6 cut(s)	401, 712, 1029, 1526, 2331, 2388
Bso31I	GGTCTC	1 cut(s)	131
Bsp119I	TTCGAA	2 cut(s)	493, 1278
Bsp120I	GGGCCC	1 cut(s)	1027
Bsp1286I	GDGCHC	2 cut(s)	1031, 2126
Bsp19I	CCATGG	1 cut(s)	2383
BspACI	CCGC	6 cut(s)	411, 443, 525, 718, 721, 793
BspANI	GGCC	6 cut(s)	401, 712, 1029, 1526, 2331, 2388
BspCNI	CTCAG	3 cut(s)	22, 727, 851
BspFNI	CGCG	1 cut(s)	416
BspLI	GGNNCC	8 cut(s)	670, 726, 812, 1028, 1029, 1251, 1696, 1883
BspPI	GGATC	3 cut(s)	307, 1816, 2069
BspQI	GCTCTTC	1 cut(s)	1174
BspT104I	TTCGAA	2 cut(s)	493, 1278
BspT107I	GGYRCC	3 cut(s)	668, 724, 1249
BspTI	CTTAAG	2 cut(s)	1498, 1733
BspTNI	GGTCTC	1 cut(s)	131
BsrI	ACTGG	5 cut(s)	1298, 1573, 1921, 2093, 2380
BssECI	CCNNGG	5 cut(s)	1016, 1030, 1487, 1690, 2383
BssNI	GRCGYC	3 cut(s)	80, 297, 1787
BssT1I	CCWWGG	2 cut(s)	1016, 2383
Bst2UI	CCWGG	3 cut(s)	1032, 1488, 1692
Bst4CI	ACNGT	6 cut(s)	514, 1232, 1795, 1974, 2032, 2046
Bst6I	CTCTTC	3 cut(s)	275, 576, 1174
BstACI	GRCGYC	3 cut(s)	80, 297, 1787
BstAFI	CTTAAG	2 cut(s)	1498, 1733
BstAPI	GCANNNNNTGC	1 cut(s)	533
BstBAI	YACGTR	1 cut(s)	552
BstBI	TTCGAA	2 cut(s)	493, 1278
BstC8I	GCNNGC	2 cut(s)	945, 1190
BstDEI	CTNAG	5 cut(s)	30, 378, 714, 838, 1448
BstDSI	CCRYGG	1 cut(s)	2383
BstENI	CCTNNNNNAGG	1 cut(s)	1628
BstF5I	GGATG	5 cut(s)	875, 1077, 1294, 1583, 2101
BstFNI	CGCG	1 cut(s)	416
BstH2I	RGCGCY	1 cut(s)	838
BstHHI	GCGC	2 cut(s)	416, 837
BstMAI	GTCTC	1 cut(s)	131
BstMWI	GCNNNNNNNGC	4 cut(s)	67, 533, 718, 2047
BstNI	CCWGG	3 cut(s)	1032, 1488, 1692
BstNSI	RCATGY	1 cut(s)	2168
BstPAI	GACNNNNGTC	1 cut(s)	25
BstSCI	CCNGG	4 cut(s)	799, 1030, 1486, 1690
BstSLI	GKGCMC	1 cut(s)	1031
BstSNI	TACGTA	1 cut(s)	552
BstUI	CGCG	1 cut(s)	416
BstV1I	GCAGC	8 cut(s)	18, 82, 156, 539, 1062, 1165, 1693, 1736
BstV2I	GAAGAC	2 cut(s)	353, 1901
BstX2I	RGATCY	1 cut(s)	312
BstXI	CCANNNNNNTGG	3 cut(s)	252, 821, 2213
BstYI	RGATCY	1 cut(s)	312
BsuI	GTATCC	2 cut(s)	965, 2372
BsuRI	GGCC	6 cut(s)	401, 712, 1029, 1526, 2331, 2388
BtgI	CCRYGG	1 cut(s)	2383
BtsCI	GGATG	5 cut(s)	875, 1077, 1294, 1583, 2101
BtsIMutI	CAGTG	3 cut(s)	846, 1914, 2373
Cac8I	GCNNGC	2 cut(s)	945, 1190
CaiI	CAGNNNCTG	1 cut(s)	89
CfoI	GCGC	2 cut(s)	416, 837
Cfr13I	GGNCC	5 cut(s)	710, 902, 1027, 1028, 1525
CseI	GACGC	1 cut(s)	69
Csp6I	GTAC	7 cut(s)	190, 541, 553, 586, 669, 725, 1975
CviQI	GTAC	7 cut(s)	190, 541, 553, 586, 669, 725, 1975
DdeI	CTNAG	5 cut(s)	30, 378, 714, 838, 1448
EaeI	YGGCCR	3 cut(s)	399, 2329, 2386
Eam1104I	CTCTTC	3 cut(s)	275, 576, 1174
EarI	CTCTTC	3 cut(s)	275, 576, 1174
Eco105I	TACGTA	1 cut(s)	552
Eco130I	CCWWGG	2 cut(s)	1016, 2383
Eco24I	GRGCYC	1 cut(s)	1031
Eco31I	GGTCTC	1 cut(s)	131
Eco47I	GGWCC	1 cut(s)	902
Eco47III	AGCGCT	1 cut(s)	836
Eco57I	CTGAAG	2 cut(s)	600, 1942
EcoNI	CCTNNNNNAGG	1 cut(s)	1628
EcoO109I	RGGNCCY	3 cut(s)	902, 1027, 1525
EcoRI	GAATTC	1 cut(s)	466
EcoRII	CCWGG	3 cut(s)	1030, 1486, 1690
EcoT14I	CCWWGG	2 cut(s)	1016, 2383
EcoT38I	GRGCYC	1 cut(s)	1031
ErhI	CCWWGG	2 cut(s)	1016, 2383
FaqI	GGGAC	3 cut(s)	389, 1402, 2079
FauI	CCCGC	1 cut(s)	714
FauNDI	CATATG	1 cut(s)	1979
FbaI	TGATCA	2 cut(s)	2098, 2248
FblI	GTMKAC	3 cut(s)	1473, 1790, 2008
FokI	GGATG	5 cut(s)	862, 1084, 1281, 1570, 2108
FriOI	GRGCYC	1 cut(s)	1031
FspBI	CTAG	3 cut(s)	408, 1017, 2136
GlaI	GCGC	2 cut(s)	415, 836
GsuI	CTGGAG	2 cut(s)	1100, 1201
HaeII	RGCGCY	1 cut(s)	838
HaeIII	GGCC	6 cut(s)	401, 712, 1029, 1526, 2331, 2388
HapII	CCGG	2 cut(s)	360, 800
HgaI	GACGC	1 cut(s)	69
HhaI	GCGC	2 cut(s)	416, 837
Hin1I	GRCGYC	3 cut(s)	80, 297, 1787
Hin6I	GCGC	2 cut(s)	414, 835
HinP1I	GCGC	2 cut(s)	414, 835
HincII	GTYRAC	4 cut(s)	151, 1037, 1132, 1668
HindII	GTYRAC	4 cut(s)	151, 1037, 1132, 1668
HindIII	AAGCTT	2 cut(s)	59, 1873
HpaI	GTTAAC	1 cut(s)	1668
HpaII	CCGG	2 cut(s)	360, 800
HphI	GGTGA	9 cut(s)	107, 253, 374, 437, 815, 1164, 1583, 1612, 2291
Hpy166II	GTNNAC	8 cut(s)	151, 365, 1037, 1132, 1474, 1668, 1791, 2009
Hpy188III	TCNNGA	8 cut(s)	310, 508, 560, 637, 905, 1180, 1438, 1944
Hpy8I	GTNNAC	8 cut(s)	151, 365, 1037, 1132, 1474, 1668, 1791, 2009
Hpy99I	CGWCG	3 cut(s)	299, 302, 1789
HpyAV	CCTTC	8 cut(s)	738, 996, 1047, 1262, 1266, 2056, 2306, 2331
HpyCH4III	ACNGT	6 cut(s)	514, 1232, 1795, 1974, 2032, 2046
HpyCH4IV	ACGT	5 cut(s)	297, 551, 1461, 1598, 1787
HpyF10VI	GCNNNNNNNGC	4 cut(s)	67, 533, 718, 2047
HpyF3I	CTNAG	5 cut(s)	30, 378, 714, 838, 1448
HpySE526I	ACGT	5 cut(s)	297, 551, 1461, 1598, 1787
Hsp92I	GRCGYC	3 cut(s)	80, 297, 1787
HspAI	GCGC	2 cut(s)	414, 835
KpnI	GGTACC	2 cut(s)	672, 728
Ksp22I	TGATCA	2 cut(s)	2098, 2248
KspAI	GTTAAC	1 cut(s)	1668
LguI	GCTCTTC	1 cut(s)	1174
LmnI	GCTCC	1 cut(s)	1633
Lsp1109I	GCAGC	8 cut(s)	18, 82, 156, 539, 1062, 1165, 1693, 1736
LweI	GCATC	9 cut(s)	471, 607, 613, 884, 1062, 1303, 1512, 1592, 1952
MaeI	CTAG	3 cut(s)	408, 1017, 2136
MaeII	ACGT	5 cut(s)	297, 551, 1461, 1598, 1787
MaeIII	GTNAC	3 cut(s)	651, 1411, 1618
MflI	RGATCY	1 cut(s)	312
MhlI	GDGCHC	2 cut(s)	1031, 2126
MlsI	TGGCCA	1 cut(s)	2388
MluNI	TGGCCA	1 cut(s)	2388
MlyI	GAGTC	7 cut(s)	35, 315, 1384, 1419, 1492, 1733, 1856
MmeI	TCCRAC	4 cut(s)	283, 949, 1970, 2051
Mox20I	TGGCCA	1 cut(s)	2388
MroXI	GAANNNNTTC	1 cut(s)	2341
MscI	TGGCCA	1 cut(s)	2388
MslI	CAYNNNNRTG	7 cut(s)	1625, 1982, 2163, 2169, 2190, 2211, 2325
Msp20I	TGGCCA	1 cut(s)	2388
MspA1I	CMGCKG	1 cut(s)	718
MspCI	CTTAAG	2 cut(s)	1498, 1733
MspI	CCGG	2 cut(s)	360, 800
MspR9I	CCNGG	4 cut(s)	801, 1032, 1488, 1692
Mva1269I	GAATGC	1 cut(s)	1387
MvaI	CCWGG	3 cut(s)	1032, 1488, 1692
MvnI	CGCG	1 cut(s)	416
MwoI	GCNNNNNNNGC	4 cut(s)	67, 533, 718, 2047
NciI	CCSGG	1 cut(s)	801
NcoI	CCATGG	1 cut(s)	2383
NdeI	CATATG	1 cut(s)	1979
NlaIV	GGNNCC	8 cut(s)	670, 726, 812, 1028, 1029, 1251, 1696, 1883
NmeAIII	GCCGAG	1 cut(s)	1792
NmuCI	GTSAC	2 cut(s)	1411, 1618
NspI	RCATGY	1 cut(s)	2168
NspV	TTCGAA	2 cut(s)	493, 1278
PciI	ACATGT	1 cut(s)	2164
PciSI	GCTCTTC	1 cut(s)	1174
PctI	GAATGC	1 cut(s)	1387
PdmI	GAANNNNTTC	1 cut(s)	2341
PfeI	GAWTC	5 cut(s)	37, 356, 1354, 1947, 1993
PflMI	CCANNNNNTGG	2 cut(s)	1048, 1259
PleI	GAGTC	7 cut(s)	34, 314, 1384, 1418, 1491, 1733, 1856
PpsI	GAGTC	7 cut(s)	34, 314, 1384, 1418, 1491, 1733, 1856
Ppu21I	YACGTR	1 cut(s)	552
PpuMI	RGGWCCY	1 cut(s)	902
PscI	ACATGT	1 cut(s)	2164
PshAI	GACNNNNGTC	1 cut(s)	25
PshBI	ATTAAT	2 cut(s)	1425, 2148
PsiI	TTATAA	1 cut(s)	1749
Psp1406I	AACGTT	1 cut(s)	1598
Psp5II	RGGWCCY	1 cut(s)	902
Psp6I	CCWGG	3 cut(s)	1030, 1486, 1690
PspGI	CCWGG	3 cut(s)	1030, 1486, 1690
PspN4I	GGNNCC	8 cut(s)	670, 726, 812, 1028, 1029, 1251, 1696, 1883
PspOMI	GGGCCC	1 cut(s)	1027
PspPI	GGNCC	5 cut(s)	710, 902, 1027, 1028, 1525
PspPPI	RGGWCCY	1 cut(s)	902
PsrI	GAACNNNNNNTAC	2 cut(s)	2363, 2395
PstNI	CAGNNNCTG	1 cut(s)	89
PsuI	RGATCY	1 cut(s)	312
RsaI	GTAC	7 cut(s)	191, 542, 554, 587, 670, 726, 1976
RsaNI	GTAC	7 cut(s)	190, 541, 553, 586, 669, 725, 1975
RseI	CAYNNNNRTG	7 cut(s)	1625, 1982, 2163, 2169, 2190, 2211, 2325
SapI	GCTCTTC	1 cut(s)	1174
Sau96I	GGNCC	5 cut(s)	710, 902, 1027, 1028, 1525
ScaI	AGTACT	1 cut(s)	587
SchI	GAGTC	7 cut(s)	35, 315, 1384, 1419, 1492, 1733, 1856
ScrFI	CCNGG	4 cut(s)	801, 1032, 1488, 1692
SduI	GDGCHC	2 cut(s)	1031, 2126
SfaNI	GCATC	9 cut(s)	471, 607, 613, 884, 1062, 1303, 1512, 1592, 1952
SfuI	TTCGAA	2 cut(s)	493, 1278
SinI	GGWCC	1 cut(s)	902
SmiMI	CAYNNNNRTG	7 cut(s)	1625, 1982, 2163, 2169, 2190, 2211, 2325
SmlI	CTYRAG	2 cut(s)	1498, 1733
SmoI	CTYRAG	2 cut(s)	1498, 1733
SnaBI	TACGTA	1 cut(s)	552
SsiI	CCGC	6 cut(s)	411, 443, 525, 718, 721, 793
SspI	AATATT	2 cut(s)	982, 1316
SspMI	CTAG	3 cut(s)	408, 1017, 2136
StyD4I	CCNGG	4 cut(s)	799, 1030, 1486, 1690
StyI	CCWWGG	2 cut(s)	1016, 2383
TaaI	ACNGT	6 cut(s)	514, 1232, 1795, 1974, 2032, 2046
TaiI	ACGT	5 cut(s)	300, 554, 1464, 1601, 1790
TatI	WGTACW	2 cut(s)	585, 1974
TauI	GCSGC	2 cut(s)	414, 721
TfiI	GAWTC	5 cut(s)	37, 356, 1354, 1947, 1993
TscAI	CASTG	3 cut(s)	846, 1921, 2380
TseFI	GTSAC	2 cut(s)	1411, 1618
TseI	GCWGC	8 cut(s)	6, 70, 169, 527, 1075, 1153, 1706, 1724
Tsp45I	GTSAC	2 cut(s)	1411, 1618
TspGWI	ACGGA	1 cut(s)	30
TspRI	CASTG	3 cut(s)	846, 1921, 2380
Van91I	CCANNNNNTGG	2 cut(s)	1048, 1259
Vha464I	CTTAAG	2 cut(s)	1498, 1733
VpaK11BI	GGWCC	1 cut(s)	902
VspI	ATTAAT	2 cut(s)	1425, 2148
XagI	CCTNNNNNAGG	1 cut(s)	1628
XapI	RAATTY	4 cut(s)	466, 1045, 1207, 1338
XceI	RCATGY	1 cut(s)	2168
XcmI	CCANNNNNNNNNTGG	3 cut(s)	249, 1484, 2183
XmaJI	CCTAGG	1 cut(s)	1016
XmiI	GTMKAC	3 cut(s)	1473, 1790, 2008
XmnI	GAANNNNTTC	1 cut(s)	2341
XspI	CTAG	3 cut(s)	408, 1017, 2136
ZraI	GACGTC	2 cut(s)	298, 1788
ZrmI	AGTACT	1 cut(s)	587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.